A cell culture device with apoptotic cell elimination function

Abstract

The present invention relates to the field of cell culture technology, and specifically to a cell culture device with an apoptotic cell removal function, the cell culture device comprising a chassis and a waste extraction device, the waste extraction device being arranged on the outside of the chassis, a lifting chamber being arranged at the lower end of the interior of the chassis, and a centrifugal chamber being arranged at the upper end of the interior of the chassis. Compared with the current cell culture devices provided with a separation tank and a liquid aspiration mechanism, the present invention can remove apoptotic cells at any time during the culture process and supplement the nutrients required for the cells in the culture bottle, so as to ensure that the concentration and number of live cells in the culture bottle can reach the best in the end. The present invention is also provided with a sealing component and a filtering mechanism, and the two sets of sealing components are used to prevent the environment in the lifting chamber and the culture chamber from being polluted when the staff places the culture bottle on the support seat, and the filtering mechanism can automatically clean the filter tube according to the air pressure change in the impurity removal chamber.

Description

Technical Field

[0001] The invention relates to the technical field of cell culture, in particular to a cell culture device with the function of eliminating apoptotic cells. Background Art

[0002] A cell culture device is a device used to culture cells in vitro, and is usually used in scientific research, drug development, bioengineering and other fields. A cell culture device usually includes culture dishes, culture bottles, incubators, cell culture medium, growth factors, CO2 incubators, cell separation tools, etc. During the cell culture process, cells grow and reproduce under appropriate temperature, humidity, gas atmosphere and nutritional conditions in order to carry out various experiments or production work.

[0003] At present, it is difficult to avoid cell apoptosis in the process of culturing cells in cell culture devices, and the substances produced by cells after apoptosis inhibit the normal production of living cells. Therefore, it is often necessary to remove apoptotic cells during cell culture. The operation method can refer to the patent "Application No. CN201910445576.1 A separation solution for removing dead liver cells and a method for removing dead liver cells using the same" and "Application No. CN202011014193.8 A method for removing dead cells in a cell suspension". However, the existing methods for removing apoptotic cells basically require manual step-by-step operation. Each time the operation is performed, the cells are easily affected by the external environment and damaged; at the same time, the cell culture device needs to be opened and closed back and forth during the process of taking out and placing the cell culture bottle. In this process, various impurities in the external environment can easily enter the cell culture device with the flow of gas; finally, the filtration system used in the current cell culture device usually requires regular maintenance, which indirectly increases the labor intensity of the staff. Summary of the invention

[0004] The purpose of the present invention is to provide a cell culture device with apoptotic cell elimination function to solve the problems raised in the above background technology.

[0005] In order to solve the above technical problems, the present invention provides the following technical solutions: a cell culture device with apoptotic cell removal function, the cell culture device comprises a chassis and a waste extraction device, the waste extraction device is arranged on the outside of the chassis, a lifting cavity is arranged at the lower end of the interior of the chassis, a centrifugal cavity is arranged at the upper end of the interior of the chassis, a culture cavity is arranged at the middle position of the interior of the chassis, a hatch is arranged at the outer end of the lifting cavity, a glass observation window is arranged at the outer end of the culture cavity, two groups of mounting seats are arranged oppositely at the left and right ends of the interior of the lifting cavity, and a linear motor is arranged at one end of the two groups of mounting seats close to each other. A support seat is arranged between the two groups of linear motors. When the present invention is not working, the support seat is located at the inner bottom end of the lifting chamber. When cells need to be cultured, the staff opens the hatch and then places the culture bottle containing cells and culture fluid on the support seat. The linear motor drives the support seat and the support seat to move upward until the culture bottle enters the culture chamber. A separation tank is arranged at the inner middle position of the centrifugal chamber. The separation tank is movably installed in the centrifugal chamber through a slider and a slide groove. A liquid suction mechanism is arranged inside the separation tank. A separation motor is arranged above the chassis. The separation motor is driven by a first transmission gear assembly. The waste extraction device is connected to the separation tank, and is connected to the separation tank through a liquid extraction tube. When the cells are cultured, the liquid suction mechanism can be intermittently opened, and the cells and culture fluid in the culture chamber can be sucked into the separation tank through the liquid suction mechanism, and then the separation tank is driven to rotate by the separation motor. Due to the different densities of apoptotic cells and living cells, apoptotic cells and living cells will produce stratification. At this time, the stratified living cells are re-transported into the culture bottle through the liquid suction mechanism, and the apoptotic cells in the separation tank are taken away by the waste extraction device. The present invention also has relatively arranged left and right ends inside the culture chamber. The fluid replenishment mechanism and the gas replenishment mechanism are used each time after the staff separates the apoptotic cells from the living cells. The staff need to replenish the culture fluid into the culture bottle through the fluid replenishment mechanism, and replenish the carbon dioxide and other gases into the culture bottle through the gas replenishment mechanism (the functions realized by the fluid replenishment mechanism and the gas replenishment mechanism belong to the conventional technical means in this field, and the specific structure is not described), so as to avoid the lack of nutrients required for the cell reproduction in the culture bottle. Compared with the current cell culture equipment, the present invention can remove apoptotic cells at any time during the culture process and replenish the nutrients required by the cells in the culture bottle, so as to ensure that the concentration and number of living cells in the culture bottle can reach the best in the end.

[0006] Furthermore, a separation chamber and a liquid suction chamber are provided inside the separation tank, and the separation chamber and the liquid suction chamber are connected through a first liquid guide hole and a second liquid guide hole. The liquid suction mechanism includes a liquid guide tube, a first electromagnet, a liquid suction plate and a stirring rod. When the culture bottle in the present invention enters the culture chamber, the stirring rod will enter the culture bottle. When the apoptotic cells are not separated from the living cells, the separation motor drives the separation tank and the stirring rod to rotate slowly through the first transmission gear assembly. At this time, the culture fluid in the culture bottle is shaken by the stirring rod, so as to increase the cell reproduction rate. Finally, the stirring rod in the present invention is a hollow structure, one end of the stirring rod is arranged in the separation chamber and connected with the first liquid guide hole, and the other end of the stirring rod extends into the culture chamber. The liquid guide tube is arranged in the separation chamber and is connected with the second liquid guide hole, the first electromagnet is fixedly installed at the upper end of the interior of the liquid suction chamber, the liquid suction plate is movably installed at the lower end of the interior of the liquid suction chamber through a sliding rod, the end of the liquid suction plate close to the first electromagnet is magnetic, and the liquid suction plate is controlled to rise and fall in the liquid suction chamber by the first electromagnet, a reversing groove is also arranged between the separation chamber and the liquid suction chamber, a second electromagnet and a reversing plate are arranged in the reversing groove, the end of the reversing plate close to the second electromagnet is magnetic, and the reversing plate is controlled to move horizontally in the reversing groove by the second electromagnet, the reversing plate is movably installed in the reversing groove by a compression spring, and a first through hole and a second through hole are arranged on the reversing plate. The present invention can transport the culture fluid and cells in the culture bottle to When in the separation tank, the reversing plate is first driven to move by the second electromagnet until the first through hole is aligned with the first liquid guide hole (at this time, the second through hole and the second liquid guide hole are staggered), and then the staff controls the liquid pipette plate to move up by the first electromagnet. At this time, under the action of air pressure, the culture fluid and cells in the culture bottle will enter the reversing groove, and then the reversing plate is driven to move by the second electromagnet until the second through hole is aligned with the second liquid guide hole (at this time, the first through hole and the first liquid guide hole are staggered), and then the staff controls the liquid pipette plate to move down by the first electromagnet to transport the culture fluid and cells in the reversing groove to the separation tank. Finally, the staff can repeat the above steps until the culture fluid and cells in the culture bottle are transported to the separation tank, so as to facilitate The staff subsequently drives the separation tank to rotate through the separation motor, and then separates the apoptotic cells and the living cells under the action of centrifugal force. After the apoptotic cells and the living cells in the separation tank are separated, the apoptotic cells will float on the upper end of the separation tank due to density. At this time, the staff will alternately turn on the first electromagnet and the second electromagnet again to transport the living cells at the lower end of the separation tank back to the culture bottle for cultivation (the working steps are as follows: first, the second electromagnet drives the reversing plate to move so that the second through hole is aligned with the second liquid guide hole, and then the first electromagnet controls the liquid absorption plate to move up. At this time, the culture fluid and cells at the lower end of the separation tank will enter the reversing groove, and then the second electromagnet drives the reversing plate to move so that the first through hole is aligned with the first liquid guide hole.Then the staff controls the liquid aspirating plate to move downward through the first electromagnet, so that the culture fluid and cells in the reversing tank can be transported to the culture bottle).

[0007] Furthermore, a waste channel is provided on the outside of the liquid aspiration chamber, and a waste pipe is also provided inside the separation chamber. One end of the waste channel is connected to the waste pipe, and the other end of the waste channel is connected to the waste extraction device through the liquid extraction tube. When the apoptotic cells and the living cells are separated, the apoptotic cells will remain in the separation tank, and the living cells will re-enter the culture bottle for culture. At this time, the staff can extract the apoptotic cells and culture fluid remaining in the separation tank through the waste extraction device, so as to prepare for the subsequent removal of the apoptotic cells. Compared with the existing cell culture equipment, the present invention can remove apoptotic cells continuously for multiple times within one culture cycle, thereby increasing the concentration and number of living cells after the end of the culture.

[0008] Furthermore, a fixed sleeve is provided between the culture chamber and the centrifugal chamber, the stirring rod passes through the fixed sleeve, a third electromagnet is provided at the inner upper end of the fixed sleeve, a sealing sleeve is provided below the third electromagnet, the end of the sealing sleeve close to the third electromagnet is magnetic, and the sealing sleeve is driven to move in the fixed sleeve by the third electromagnet, the inner lower end of the fixed sleeve is respectively connected with the liquid replenishment mechanism and the gas replenishment mechanism through the first conduit and the second conduit, and a plurality of groups of feed holes are provided at the end of the stirring rod close to the first conduit and the second conduit. In the present invention, when the culture fluid and cells in the culture bottle are transported to the separation tank, or the culture fluid and cells in the separation tank are transported to the culture bottle, the third electromagnet will drive the sealing sleeve to move to the inner lower end of the fixed sleeve, and the plurality of groups of feed holes are blocked by the sealing sleeve to avoid affecting the operation of the liquid suction mechanism. If the liquid suction mechanism is not working, the third electromagnet will drive the sealing sleeve to move to the inner upper end of the fixed sleeve, and at this time, the culture fluid and gas required for cell production can be transported to the culture bottle through the liquid replenishment mechanism and the gas replenishment mechanism to ensure that the nutrients required for cell production are sufficient.

[0009] Furthermore, a group of sealing components is provided at the upper end of the interior of each group of the mounting seats, a group of cavities is provided inside each group of the mounting seats, both groups of cavities are connected with the lifting chamber, a circulation chamber is also provided inside the chassis, a circulation fan is provided at the upper end of the interior of the circulation chamber, the rotating shaft of the circulation fan can be connected with the separation motor through a gear assembly, so as to reduce the setting of the driving device, a purification box is provided at the middle position inside the circulation chamber, a debris removal chamber and a sterilization chamber are provided inside the purification box, a filtering mechanism is provided inside the debris removal chamber, a partition is provided inside the sterilization chamber, and each group of partitions is provided with several groups of sterilization lamps, one end of the debris removal chamber is connected with the air inlet end of the circulation fan, the other end of the debris removal chamber is connected with one of the groups of cavities through an air inlet pipe, one end of the sterilization chamber is connected with the air outlet end of the circulation fan, and the other end of the sterilization chamber is connected The air outlet pipe is connected to another group of cavities. When the present invention is not working, the lifting chamber and the culture chamber are separated by the two groups of sealing components. When the staff places the culture bottle on the support seat, the lifting chamber and the culture chamber are still in a separated state. At this time, the staff needs to close the cabin door and then turn on the circulating fan. The gas in the lifting chamber can be sucked away by the circulating fan and flows along the cavity-decontamination chamber-circulating fan-sterilization chamber-cavity. The gas in the lifting chamber can be purified by the filtering mechanism in the decontamination chamber and several groups of sterilization lamps in the sterilization chamber. When the gas in the lifting chamber is purified, the sealing component is opened. At this time, the lifting chamber and the culture chamber are connected to facilitate the culture bottle to enter the culture chamber. Through the above technical solution, when the staff places the culture bottle on the support seat, the environment in the lifting chamber and the culture chamber will not be polluted, thereby ensuring the success rate of cell culture.

[0010] Furthermore, the sealing assembly includes a cylinder, a transmission groove and a sealing groove, a transmission plate is arranged inside the transmission groove, a sealing plate is arranged inside the sealing groove, the cylinder is connected to the transmission plate, the upper and lower ends of the transmission groove are respectively connected with the left and right ends of the sealing groove, and the present invention drives the sealing plate to move left and right in the sealing groove by the cylinder. When the two groups of the sealing plates are close to each other, the lifting chamber and the culture chamber are separated, thereby ensuring that the culture bottle will not be contaminated when it is placed in the lifting chamber. When the two groups of the sealing plates are far away from each other, the lifting chamber and the culture chamber are connected, thereby ensuring that the culture bottle can smoothly enter the culture chamber.

[0011] Furthermore, the filter mechanism includes a filter tube, a first end cap and a second end cap, the first end cap being arranged at one end of the impurity removal chamber close to the circulation fan, the second end cap being arranged at one end of the impurity removal chamber close to the air inlet pipe, the filter tube being arranged between the first end cap and the second end cap, and the second end cap being arranged at one end close to the air inlet pipe with a sensing groove, the sensing groove being arranged with a sensing block and a sensing spring, and the sensing block being arranged at one end close to the sensing spring with a piezoelectric sheet. In the present invention, the gas in the lifting chamber will first enter the impurity removal chamber during the flow, and then pass through the filter tube and enter the circulation fan. In the present invention, there are several groups of filter holes on the filter tube, and solid impurities in the gas are intercepted by the filter tube. When the filter holes on the filter tube are blocked, the air pressure in the impurity removal chamber will increase. At this time, under the action of the air pressure, the piezoelectric sheet on the sensing block will generate a group of electrical signals. By monitoring the size of the electrical signals, the staff can clean the filter tube in time.

[0012] Furthermore, the filtering mechanism also includes an exhaust groove, an air pump and a rotating motor. The exhaust groove is arranged at the side end of the impurity removal chamber, and a plurality of groups of baffles are arranged on the filter tube. One end of the exhaust groove is connected to the air pump, and the other end of the exhaust groove is connected to the impurity removal chamber. The rotating motor is connected to the first end cover through a second transmission gear assembly. The rotating motor in the present invention is connected to the piezoelectric piece. When the electrical signal on the piezoelectric piece increases sharply, the first end cover and the filter tube are driven to rotate by the rotating motor. At this time, each area on the filter tube will approach the exhaust groove in turn, and the impurities trapped on the filter tube can be sucked away by the air pump, thereby achieving the purpose of cleaning the filter tube.

[0013] Compared with the prior art, the beneficial effects achieved by the present invention are as follows: compared with the current cell culture equipment, the present invention is provided with a separation tank and a liquid suction mechanism. During the normal production process of cells, the separation motor drives the separation tank and the liquid suction mechanism to rotate slowly through the first transmission gear assembly. At this time, the culture fluid in the culture bottle can be shaken by the liquid suction mechanism to increase the cell reproduction rate. If a large number of apoptotic cells appear during the cell culture process, the staff can intermittently open the liquid suction mechanism, and the cells and culture fluid in the culture chamber can be sucked into the separation tank through the liquid suction mechanism. The separation tank is then driven to rotate at a faster speed by the separation motor. Due to the different densities of apoptotic cells and living cells, apoptotic cells and living cells will be stratified. At this time, the stratified living cells are re-transported into the culture bottle by the liquid suction mechanism, and the separated cells are removed by the waste extraction device. The apoptotic cells in the separation tank are removed. The present invention is also provided with a liquid replenishing mechanism and a gas replenishing mechanism. The staff can replenish the culture fluid and gas into the culture bottle as needed, so as to avoid insufficient nutrients required for cell reproduction in the culture bottle. Compared with the current cell culture equipment, the present invention can remove the apoptotic cells at any time during the culture process and replenish the nutrients required for the cells in the culture bottle, so as to ensure that the concentration and number of live cells in the final culture bottle can reach the best. The present invention is also provided with a sealing component and a filtering mechanism. The two sets of sealing components prevent the environment in the lifting chamber and the culture chamber from being polluted when the staff places the culture bottle on the support seat, so as to ensure the success rate of cell culture. On the one hand, the filtering mechanism can remove impurities in the gas inside the lifting chamber, and on the other hand, the filtering mechanism can automatically clean the filter tube according to the air pressure changes in the impurity removal chamber. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0015] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0016] Figure 2 is a first perspective schematic diagram of the present invention;

[0017] Figure 3 is a second viewing angle schematic diagram of the present invention;

[0018] Figure 4 is an internal front view of the present invention;

[0019] Figure 5 The present invention Figure 4 Schematic diagram of the structure of the middle A part;

[0020] Figure 6 is an internal side view of the present invention;

[0021] Figure 7 It is a schematic diagram of the internal structure of the separation tank of the present invention;

[0022] Figure 8 The present invention Figure 7 Schematic diagram of the structure of the middle BB part;

[0023] Fig. 9 The present invention Figure 7 Schematic diagram of the CC structure;

[0024] Fig.10 It is a schematic diagram of the internal structure of the fixing sleeve of the present invention;

[0025] Fig.11 It is a schematic diagram of the structure of the purification box of the present invention;

[0026] Fig.12 The present invention Fig.11 Schematic diagram of the middle DD structure;

[0027] Fig.13 The present invention Fig.12 Schematic diagram of the EE structure.

[0028] In the figure: 1, chassis; 11, hatch; 12, lifting chamber; 121, mounting seat; 1211, cavity; 122, linear motor; 123, support seat; 124, cylinder; 125, transmission groove; 126, sealing plate; 127, sealing groove; 13, culture chamber; 131, liquid replenishment mechanism; 132, gas replenishment mechanism; 14, centrifugal chamber; 141, separation tank; 1411, separation chamber; 1412, first liquid guide hole; 1413, second liquid guide hole; 14131, liquid guide tube; 1414, liquid suction chamber; 1415, first electromagnet; 1416, waste channel; 14161, waste tube; 1417, liquid suction plate; 14 18. Reversing groove; 14181. Second electromagnet; 1419. Reversing plate; 14191. First through hole; 14192. Second through hole; 15. Circulation chamber; 151. Circulation fan; 152. Purification box; 1521. De-impurity chamber; 1522. Sterilization chamber; 1523. Filter tube; 1524. First end cover; 1525. Second end cover; 1526. Exhaust groove; 1527. Air pump; 1528. Rotating motor; 153. Inlet pipe; 154. Outlet pipe; 16. Stirring rod; 17. Fixed sleeve; 171. Third electromagnet; 172. Sealing sleeve; 2. Separation motor; 3. Waste extraction device; 4. Culture bottle. DETAILED DESCRIPTION

[0029] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application can be combined with each other. The present invention will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0030] See also Figure 1-Figure 13 The present invention provides a technical solution: a cell culture device with apoptotic cell elimination function, the cell culture device includes a chassis 1 and a waste extraction device 3, the waste extraction device 3 is arranged on the outside of the chassis 1, a lifting chamber 12 is arranged at the lower end of the interior of the chassis 1, a centrifugal chamber 14 is arranged at the upper end of the interior of the chassis 1, a culture chamber 13 is arranged at the middle position of the interior of the chassis 1, a hatch 11 is arranged at the outer end of the lifting chamber 12, a glass observation window is arranged at the outer end of the culture chamber 13, two groups of mounting seats 121 are arranged oppositely at the left and right ends of the interior of the lifting chamber 12, a linear motor 122 is arranged at one end of the two groups of mounting seats 121 close to each other, and a The support seat 123 is located at the bottom end of the lifting chamber 12 when the present invention is not working. When the cells need to be cultured, the staff opens the hatch 11 and then places the culture bottle 4 containing the cells and the culture fluid on the support seat 123. The support seat 123 and the support seat 123 are driven upward by the linear motor 122 until the culture bottle 4 enters the culture chamber 13. A separation tank 141 is provided at the middle position inside the centrifugal chamber 14. The separation tank 141 is movably installed in the centrifugal chamber 14 through a slider and a slide groove. A liquid suction mechanism is provided inside the separation tank 141. A separation motor 2 is provided above the chassis 1. The separation motor 2 is driven by a first transmission gear set. The waste extraction device 3 is connected to the separation tank 141 through the liquid extraction tube. When the cells are cultured, the present invention can intermittently open the liquid suction mechanism, and the cells and culture fluid in the culture chamber 13 can be sucked into the separation tank 141 through the liquid suction mechanism, and then the separation tank 141 is driven to rotate by the separation motor 2. Due to the different densities of apoptotic cells and living cells, apoptotic cells and living cells will produce stratification. At this time, the stratified living cells are re-transported to the culture bottle 4 through the liquid suction mechanism, and the apoptotic cells in the separation tank 141 are taken away by the waste extraction device 3. The present invention also has relatively arranged liquid replenishing devices at the left and right ends inside the culture chamber 13. Mechanism 131 and gas replenishing mechanism 132. Each time after the staff separates the apoptotic cells and the living cells, they need to replenish the culture fluid into the culture bottle 4 through the liquid replenishing mechanism 131, and replenish the carbon dioxide and other gases into the culture bottle 4 through the gas replenishing mechanism 132 (the functions realized by the liquid replenishing mechanism 131 and the gas replenishing mechanism 132 belong to the conventional technical means in this field, and the specific structure is not described), so as to avoid the lack of nutrients required for the reproduction of the cells in the culture bottle 4. Compared with the current cell culture equipment, the present invention can remove the apoptotic cells at any time during the culture process and replenish the nutrients required by the cells in the culture bottle 4, so as to ensure that the concentration and number of living cells in the culture bottle 4 can reach the best.

[0031] like Figure 4 , Figure 6-Figure 9As shown, the interior of the separation tank 141 is provided with a separation chamber 1411 and a liquid suction chamber 1414, and the separation chamber 1411 and the liquid suction chamber 1414 are connected through a first liquid guide hole 1412 and a second liquid guide hole 1413, and the liquid suction mechanism includes a liquid guide tube 14131, a first electromagnet 1415, a liquid suction plate 1417 and a stirring rod 16. When the culture bottle 4 of the present invention enters the culture chamber 13, the stirring rod 16 will enter the culture bottle 4. When the apoptotic cells are not separated from the living cells, the separation motor 2 drives the separation tank 141 and the stirring rod 16 to rotate slowly through the first transmission gear assembly. At this time, the culture liquid in the culture bottle 4 is shaken by the stirring rod 16, so as to increase the cell propagation speed. Finally, the stirring rod 16 of the present invention The rod 16 is a hollow structure. One end of the stirring rod 16 is arranged in the separation chamber 1411 and is connected to the first liquid guide hole 1412. The other end of the stirring rod 16 extends into the culture chamber 13. The liquid guide tube 14131 is arranged in the separation chamber 1411 and is connected to the second liquid guide hole 1413. The first electromagnet 1415 is fixedly installed on the upper end of the interior of the liquid suction chamber 1414. The liquid suction plate 1417 is movably installed on the lower end of the interior of the liquid suction chamber 1414 through a sliding rod. The end of the liquid suction plate 1417 close to the first electromagnet 1415 is magnetic. The liquid suction plate 1417 is controlled to rise and fall in the liquid suction chamber 1414 by the first electromagnet 1415. A reversing groove 1418 is also arranged between the separation chamber 1411 and the liquid suction chamber 1414. The reversing groove 1418 is fixedly installed on the upper end of the interior of the liquid suction chamber 1414. The liquid suction plate 1417 is movably installed on the lower end of the interior of the liquid suction chamber 1414 through a sliding rod. The end of the liquid suction plate 1417 close to the first electromagnet 1415 is magnetic. The liquid suction plate 1417 is controlled to rise and fall in the liquid suction chamber 1414 by the first electromagnet 1415. 8 is provided with a second electromagnet 14181 and a reversing plate 1419, one end of the reversing plate 1419 close to the second electromagnet 14181 is magnetic, and the reversing plate 1419 is controlled by the second electromagnet 14181 to move horizontally in the reversing slot 1418, and the reversing plate 1419 is movably installed in the reversing slot 1418 by a compression spring, and the reversing plate 1419 is provided with a first through hole 14191 and a second through hole 14192. When the culture fluid and cells in the culture bottle 4 are transported to the separation tank 141, the reversing plate 1419 is first driven to move by the second electromagnet 14181 until the first through hole 14191 is aligned with the first liquid guide hole 1412 (at this time, the second through hole 14192 is aligned with the second liquid guide hole 1413). Phase staggered), then the staff controls the pipette plate 1417 to move up through the first electromagnet 1415, at this time, under the action of air pressure, the culture fluid and cells in the culture bottle 4 will enter the reversing groove 1418, and then the reversing plate 1419 is driven to move by the second electromagnet 14181 until the second through hole 14192 is aligned with the second liquid guide hole 1413 (at this time, the first through hole 14191 is staggered with the first liquid guide hole 1412), and then the staff controls the pipette plate 1417 to move down through the first electromagnet 1415, so that the culture fluid and cells in the reversing groove 1418 can be transported to the separation tank 141, and finally the staff can repeat the above steps until the culture fluid and cells in the culture bottle 4 are transported to the separation tank 141,This facilitates the staff to subsequently drive the separation tank 141 to rotate through the separation motor 2, and then separate the apoptotic cells and the living cells under the action of centrifugal force. After the apoptotic cells and the living cells in the separation tank 141 are separated, the apoptotic cells will float on the upper end of the separation tank 141 due to density. At this time, the staff will alternately turn on the first electromagnet 1415 and the second electromagnet 14181 again to transport the living cells at the lower end of the separation tank 141 to the culture bottle 4 for culture (the working steps are as follows: first, the second electromagnet 14181 drives the reversing plate 1419 The first electromagnet 1415 controls the liquid pipette 1417 to move upward, and the culture fluid and cells at the lower end of the separation tank 141 enter the reversing groove 1418. The second electromagnet 14181 then drives the reversing plate 1419 to move, so that the first through hole 14191 is aligned with the first liquid guide hole 1412. The staff then controls the liquid pipette 1417 to move downward through the first electromagnet 1415, and the culture fluid and cells in the reversing groove 1418 can be transported to the culture bottle 4).

[0032] like Figure 4 , Figure 6-Figure 8 As shown, a waste channel 1416 is provided on the outside of the liquid suction chamber 1414, and a waste tube 14161 is also provided inside the separation chamber 1411. One end of the waste channel 1416 is connected to the waste tube 14161, and the other end of the waste channel 1416 is connected to the waste extraction device 3 through the liquid suction tube. When the apoptotic cells and the living cells are separated at the end of the present invention, the apoptotic cells will remain in the separation tank 141, and the living cells will re-enter the culture bottle 4 for culture. At this time, the staff can extract the apoptotic cells and culture fluid remaining in the separation tank 141 through the waste extraction device 3, so as to prepare for the subsequent removal of the apoptotic cells. Compared with the existing cell culture equipment, the present invention can remove apoptotic cells continuously for multiple times within a culture cycle, thereby increasing the concentration and number of living cells after the end of the culture.

[0033] like Figure 4 , Figure 6 and Fig.10As shown, a fixed sleeve 17 is arranged between the culture chamber 13 and the centrifugal chamber 14, and a stirring rod 16 runs through the fixed sleeve 17. A third electromagnet 171 is arranged at the upper end of the interior of the fixed sleeve 17, and a sealing sleeve 172 is arranged below the third electromagnet 171. The end of the sealing sleeve 172 close to the third electromagnet 171 has magnetism, and the sealing sleeve 172 is driven by the third electromagnet 171 to move in the fixed sleeve 17. The lower end of the interior of the fixed sleeve 17 is connected to the liquid replenishment mechanism 131 and the gas replenishment mechanism 132 through the first conduit and the second conduit, respectively. A plurality of feeding holes are arranged at one end of the stirring rod 16 close to the first conduit and the second conduit. When the culture fluid and cells in the culture bottle 4 are transported to the separation tank 141, or when the culture fluid and cells in the separation tank 141 are transported to the culture bottle 4, the third electromagnet 171 will drive the sealing sleeve 172 to move to the inner lower end of the fixed sleeve 17, and block several groups of feed holes through the sealing sleeve 172 to avoid affecting the operation of the liquid suction mechanism. If the liquid suction mechanism is not working, the third electromagnet 171 will drive the sealing sleeve 172 to move to the inner upper end of the fixed sleeve 17. At this time, the culture fluid and gas required for cell production can be transported to the culture bottle 4 through the liquid replenishment mechanism 131 and the gas replenishment mechanism 132 to ensure that there are sufficient nutrients required for cell production.

[0034] like Figure 4 , Figure 5 , Figure 10-13As shown, a group of sealing components are arranged at the upper end of each group of mounting seats 121, a group of cavities 1211 are arranged inside each group of mounting seats 121, and both groups of cavities 1211 are connected to the lifting chamber 12. A circulation chamber 15 is also arranged inside the chassis 1, and a circulation fan 151 is arranged at the upper end of the circulation chamber 15. The rotating shaft of the circulation fan 151 can be connected to the separation motor 2 through a gear assembly, so as to reduce the setting of the driving device. A purification box 152 is arranged at the middle position inside the circulation chamber 15, and the inside of the purification box 152 A cleaning chamber 1521 and a sterilization chamber 1522 are provided. A filtering mechanism is provided inside the cleaning chamber 1521. A partition is provided inside the sterilization chamber 1522. Each group of partitions is provided with a plurality of sterilization lamps. One end of the cleaning chamber 1521 is connected to the air inlet end of the circulating fan 151. The other end of the cleaning chamber 1521 is connected to one group of cavities 1211 through an air inlet pipe 153. One end of the sterilization chamber 1522 is connected to the air outlet end of the circulating fan 151. The other end of the sterilization chamber 1522 is connected to the other group of cavities 1211 through an air outlet pipe 154. A group of cavities 1211 are connected. When the present invention is not working, the lifting chamber 12 is separated from the culture chamber 13 by two groups of sealing components. When the staff places the culture bottle 4 on the support seat 123, the lifting chamber 12 and the culture chamber 13 are still in a separated state. At this time, the staff needs to close the hatch 11 and then turn on the circulating fan 151. The circulating fan 151 can absorb the gas in the lifting chamber 12 and move it along the cavity 1211-the impurity removal chamber 1521-the circulating fan 151-the sterilization chamber 1522-the cavity 121 1 flows, and the gas in the lifting chamber 12 can be purified by the filtering mechanism in the impurity removal chamber 1521 and the several groups of sterilization lamps in the sterilization chamber 1522. When the gas in the lifting chamber 12 is purified, the sealing component is opened, and the lifting chamber 12 is connected with the culture chamber 13 to facilitate the culture bottle 4 to enter the culture chamber 13. Through the above technical solution, when the staff places the culture bottle 4 on the support seat 123, the environment in the lifting chamber 12 and the culture chamber 13 will not be polluted, thereby ensuring the success rate of cell culture.

[0035] like Figure 5 As shown, the sealing assembly includes a cylinder 124, a transmission groove 125 and a sealing groove 127. A transmission plate is arranged inside the transmission groove 125, and a sealing plate 126 is arranged inside the sealing groove 127. The cylinder 124 is connected to the transmission plate, and the upper and lower ends of the transmission groove 125 are respectively connected to the left and right ends of the sealing groove 127. The present invention drives the sealing plate 126 to move left and right in the sealing groove 127 by the cylinder 124. When the two groups of sealing plates 126 are close to each other, the lifting chamber 12 and the culture chamber 13 are separated, thereby ensuring that the culture bottle 4 will not be contaminated when it is placed in the lifting chamber 12. When the two groups of sealing plates 126 are far away from each other, the lifting chamber 12 and the culture chamber 13 are connected, thereby ensuring that the culture bottle 4 can smoothly enter the culture chamber 13.

[0036] like Figure 4 , Figure 10-13 As shown, the filtering mechanism includes a filter tube 1523, a first end cover 1524 and a second end cover 1525. The first end cover 1524 is arranged at one end of the impurity removal chamber 1521 close to the circulation fan 151, and the second end cover 1525 is arranged at one end of the impurity removal chamber 1521 close to the air inlet pipe 153. The filter tube 1523 is arranged between the first end cover 1524 and the second end cover 1525. An induction groove is arranged at one end of the second end cover 1525 close to the air inlet pipe 153. An induction block and an induction spring are arranged in the induction groove. A piezoelectric In the present invention, the gas in the lifting chamber 12 will first enter the impurity removal chamber 1521 during the flow, and then pass through the filter tube 1523 to enter the circulating fan 151. In the present invention, there are several groups of filter holes on the filter tube 1523, and the solid impurities in the gas are intercepted by the filter tube 1523. When the filter holes on the filter tube 1523 are blocked, the air pressure in the impurity removal chamber 1521 will increase. At this time, under the action of the air pressure, the piezoelectric sheet on the sensing block will generate a group of electrical signals. By monitoring the size of the electrical signals, the staff can clean the filter tube 1523 in time.

[0037] like Figure 4 , Figure 5 , Figure 10-13 As shown, the filtering mechanism also includes an exhaust groove 1526, an air pump 1527 and a rotating motor 1528. The exhaust groove 1526 is arranged at the side end of the impurity removal chamber 1521. A plurality of groups of baffles are arranged on the filter tube 1523. One end of the exhaust groove 1526 is connected to the air pump 1527, and the other end of the exhaust groove 1526 is connected to the impurity removal chamber 1521. The rotating motor 1528 is connected to the first end cover 1524 through a second transmission gear assembly. The rotating motor 1528 in the present invention is connected to the piezoelectric sheet. When the electrical signal on the piezoelectric sheet increases sharply, the first end cover 1524 and the filter tube 1523 are driven to rotate by the rotating motor 1528. At this time, each area on the filter tube 1523 will approach the exhaust groove 1526 in turn, and the impurities trapped on the filter tube 1523 can be sucked away by the air pump 1527, thereby achieving the purpose of cleaning the filter tube 1523.

[0038] The working principle of the present invention is as follows: when not in operation, the lifting chamber 12 is separated from the culture chamber 13 by two sets of sealing components. When the staff places the culture bottle 4 on the support seat 123, the lifting chamber 12 and the culture chamber 13 need to remain separated. At this time, the staff closes the hatch 11 and then turns on the circulating fan 151. The circulating fan 151 can absorb the gas in the lifting chamber 12 and flow along the cavity 1211-impurity removal chamber 1521-circulating fan 151-sterilization chamber 1522-cavity 1211. The filtering mechanism in the miscellaneous cavity 1521 and the several groups of sterilizing lamps in the sterilizing cavity 1522 can purify the gas in the lifting cavity 12. When the gas in the lifting cavity 12 is purified, the sealing component is opened, and the lifting cavity 12 is connected with the culture cavity 13. Then, the linear motor 122 drives the support seat 123 and the support seat 123 to move upward. When the culture bottle 4 enters the culture cavity 13, the stirring rod 16 enters the culture bottle 4. When the present invention does not separate the apoptotic cells from the living cells, the separation tank 141 is made to move upward by the separation motor 2. The stirring rod 16 rotates slowly, at this time, the stirring rod 16 will shake the culture solution in the culture bottle 4, so as to increase the cell reproduction speed. The separation tank 141 is provided with a suction mechanism. When the present invention is culturing cells, the suction mechanism can be intermittently opened, and the cells and culture solution in the culture chamber 13 can be sucked into the separation tank 141 through the suction mechanism, and then the separation tank 141 is driven to rotate by the separation motor 2. Due to the different densities of apoptotic cells and living cells, apoptotic cells and living cells will produce stratification. At this time, the stratified living cells are re-transported to the culture bottle 4 through the suction mechanism, and the apoptotic cells in the separation tank 141 are taken away by the waste extraction device 3. The present invention also has a liquid replenishment mechanism 131 and a gas replenishment mechanism 132 relatively arranged at the left and right ends of the culture chamber 13. After the staff separates the apoptotic cells and the living cells each time, they need to replenish the culture solution into the culture bottle 4 through the liquid replenishment mechanism 131, and replenish the gas such as carbon dioxide into the culture bottle 4 through the gas replenishment mechanism 132, so as to avoid the lack of nutrients required for cell reproduction in the culture bottle 4.

[0039] Although the embodiments of the present invention have been shown and described, it is to be understood that the above embodiments are illustrative and are not to be construed as limitations on the present invention. A person skilled in the art may change, modify, substitute and vary the above embodiments within the scope of the present invention without departing from the principles and purpose of the present invention.

Claims

1. A cell culture device with apoptotic cell elimination function, characterized in that: The cell culture device comprises a chassis (1) and a waste extraction device (3), wherein the waste extraction device (3) is arranged outside the chassis (1), a lifting chamber (12) is arranged at the lower end of the chassis (1), a centrifugal chamber (14) is arranged at the upper end of the chassis (1), a culture chamber (13) is arranged at the middle position of the chassis (1), a hatch (11) is arranged at the outer end of the lifting chamber (12), two groups of mounting seats (121) are arranged opposite to each other at the left and right ends of the lifting chamber (12), and a linear motor (122) is arranged at each end of the two groups of mounting seats (121) close to each other, and the two groups of linear motors (122) is provided with a support seat (123), a culture bottle (4) is provided on the support seat (123), a liquid replenishing mechanism (131) and a gas replenishing mechanism (132) are provided at the left and right ends of the culture chamber (13), a separation tank (141) is provided at the middle position of the centrifugal chamber (14), a liquid suction mechanism is provided inside the separation tank (141), a separation motor (2) is provided above the chassis (1), the separation motor (2) is connected to the separation tank (141) through a first transmission gear assembly, and the waste extraction device (3) is connected to the separation tank (141) through a liquid suction pipe; The separation tank (141) is provided with a separation chamber (1411) and a liquid suction chamber (1414) inside. The separation chamber (1411) and the liquid suction chamber (1414) are connected via a first liquid guide hole (1412) and a second liquid guide hole (1413). The liquid suction mechanism comprises a liquid guide tube (14131), a first electromagnet (1415), a liquid suction plate (1417) and a stirring rod (16). The stirring rod (16) is a hollow structure. One end of the stirring rod (16) is arranged in the separation chamber (1411) and is connected to the first liquid guide hole (1412). The other end of the stirring rod (16) extends into the culture chamber (13). The liquid guide tube (14131) is arranged in the separation chamber (1411). 1411) and is connected to the second liquid guide hole (1413), the first electromagnet (1415) is fixedly mounted on the upper end of the interior of the liquid suction chamber (1414), the liquid suction plate (1417) is movably mounted on the lower end of the interior of the liquid suction chamber (1414) via a sliding rod, a reversing groove (1418) is further provided between the separation chamber (1411) and the liquid suction chamber (1414), a second electromagnet (14181) and a reversing plate (1419) are provided in the reversing groove (1418), the reversing plate (1419) is movably mounted in the reversing groove (1418) via a compression spring, and a first through hole (14191) and a second through hole (14192) are provided on the reversing plate (1419); When the first through hole (14191) is aligned with the first liquid guiding hole (1412), the second through hole (14192) is staggered with the second liquid guiding hole (1413); When the second through hole (14192) is aligned with the second liquid guiding hole (1413), the first through hole (14191) is staggered with the first liquid guiding hole (1412); One end of the liquid absorption plate (1417) close to the first electromagnet (1415) is magnetic, and the liquid absorption plate (1417) is controlled to rise and fall in the liquid absorption chamber (1414) by the first electromagnet (1415); One end of the reversing plate (1419) close to the second electromagnet (14181) is magnetic, and the reversing plate (1419) is controlled by the second electromagnet (14181) to move horizontally in the reversing slot (1418), so that the first through hole (14191) is aligned with the first liquid guide hole (1412) or the second through hole (14192) is aligned with the second liquid guide hole (1413); A fixing sleeve (17) is arranged between the culture chamber (13) and the centrifugal chamber (14); the stirring rod (16) passes through the fixing sleeve (17); a third electromagnet (171) is arranged at the upper end of the fixing sleeve (17); a sealing sleeve (172) is arranged below the third electromagnet (171); the lower end of the fixing sleeve (17) is connected to the liquid replenishing mechanism (131) and the gas replenishing mechanism (132) through the first conduit and the second conduit respectively; and a plurality of groups of feed holes are arranged at one end of the stirring rod (16) close to the first conduit and the second conduit; One end of the sealing sleeve (172) close to the third electromagnet (171) is magnetic, and the sealing sleeve (172) is driven by the third electromagnet (171) to move in the fixed sleeve (17), so that the sealing sleeve (172) blocks a plurality of groups of feed holes; Each group of the mounting seats (121) is provided with a group of cavities (1211) inside, and both groups of the cavities (1211) are in communication with the lifting chamber (12). A circulation chamber (15) is also provided inside the chassis (1), and a circulation fan (151) is provided at the upper end of the circulation chamber (15). A purification box (152) is provided at the middle position inside the circulation chamber (15), and a decontamination chamber (1521) and a sterilization chamber (1522) are provided inside the purification box (152), and a decontamination chamber (1521) and a sterilization chamber (1522) are provided inside the decontamination chamber (1521). A filter mechanism, wherein a partition is provided inside the sterilization chamber (1522), and each group of partitions is provided with a plurality of groups of sterilization lamps, one end of the impurity removal chamber (1521) is connected to the air inlet end of the circulation fan (151), and the other end of the impurity removal chamber (1521) is connected to one group of cavities (1211) through an air inlet pipe (153), one end of the sterilization chamber (1522) is connected to the air outlet end of the circulation fan (151), and the other end of the sterilization chamber (1522) is connected to another group of cavities (1211) through an air outlet pipe (154); The filtering mechanism comprises a filtering tube (1523), a first end cover (1524) and a second end cover (1525), wherein the first end cover (1524) is arranged at one end of the impurity removal chamber (1521) close to the circulation fan (151), and the second end cover (1525) is arranged inside the impurity removal chamber (1521) at one end close to the air inlet pipe (153); the filtering tube (1523) is arranged between the first end cover (1524) and the second end cover (1525), and an induction groove is arranged at one end of the second end cover (1525) close to the air inlet pipe (153), wherein an induction block and an induction spring are arranged in the induction groove, and a piezoelectric sheet is arranged at one end of the induction block close to the induction spring; The filtering mechanism further comprises an exhaust groove (1526), ​​an air pump (1527) and a rotating motor (1528); the exhaust groove (1526) is arranged at a side end of the impurity removal chamber (1521); a plurality of groups of retaining bars are arranged on the filtering tube (1523); one end of the exhaust groove (1526) is connected to the air pump (1527); the other end of the exhaust groove (1526) is connected to the impurity removal chamber (1521); and the rotating motor (1528) is connected to the first end cover (1524) via a second transmission gear assembly; The rotating motor (1528) is connected to the piezoelectric sheet. When the electrical signal on the piezoelectric sheet increases sharply, the rotating motor (1528) drives the first end cover (1524) and the filter tube (1523) to rotate, so that each area on the filter tube (1523) approaches the exhaust groove (1526) in turn, and the impurities trapped on the filter tube (1523) are sucked away by the air pump (1527).

2. The cell culture device with apoptotic cell elimination function according to claim 1, characterized in that: A waste channel (1416) is arranged outside the liquid suction chamber (1414), and a waste pipe (14161) is also arranged inside the separation chamber (1411); one end of the waste channel (1416) is connected to the waste pipe (14161), and the other end of the waste channel (1416) is connected to the waste extraction device (3) via a liquid extraction pipe.

3. The cell culture device with apoptotic cell elimination function according to claim 1, characterized in that: A group of sealing components is disposed at the upper end of the interior of each group of mounting seats (121), the sealing components comprising a cylinder (124), a transmission groove (125) and a sealing groove (127), a transmission plate is disposed inside the transmission groove (125), a sealing plate (126) is disposed inside the sealing groove (127), the cylinder (124) is connected to the transmission plate, and the upper and lower ends of the transmission groove (125) are respectively connected to the left and right ends of the sealing groove (127); The sealing plate (126) is driven by the cylinder (124) to move left and right in the sealing groove (127); when the two groups of sealing plates (126) are close to each other, the lifting chamber (12) and the culture chamber (13) are separated; when the two groups of sealing plates (126) are away from each other, the lifting chamber (12) and the culture chamber (13) are connected.

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