Biological sample sampling instrument and biological sample freezing tube

Abstract

The present invention discloses a biological sample sampler and a biological sample cryopreservation tube. The biological sample sampler includes a tube cover, on which an inner tube with an open portion is axially arranged, the lower end of the open portion and the lower end of the inner tube are located in the same radial cross section, the inner cavity of the inner tube serves as a space for accommodating biological samples, the outer wall of the inner tube is provided with scale marks, and the tube cover serves as a grip for an operator to hold; the cryopreservation tube adopts the aforementioned biological sample sampler. The adoption of the present invention can not only realize the rapid sampling of biological samples, but also eliminate the freeze-thaw process in the existing method, at least fundamentally solve the problem that the freeze-thaw process affects the accuracy of the biological sample detection results, and at the same time greatly simplifies the operation process of loading biological samples.

Description

Technical Field

[0001] The invention belongs to the technical field of biological sample sampling and detection, and specifically relates to a biological sample sampler and a biological sample freezing tube. Background Art

[0002] At present, the operation process of biological sample sampling, freezing and sampling detection is mainly as follows: the obtained in vitro biological samples (such as feces, animal tissues) are usually placed in a cup body first, and then the biological samples to be frozen are placed in several conventional cryopreservation tubes with specific tools (such as spoons and tweezers) for freezing (this process is also called sampling freezing). When sampling and testing are required, the cryopreservation tubes containing biological samples are taken out and thawed, and then samples are taken in multiple times according to the amount, and the unused (remaining) biological samples are placed in cryopreservation tubes for continued freezing for the next sampling and testing. Among them, the process of thawing and freezing biological samples again is called freeze-thaw. The biological samples in the same cryopreservation tube often undergo multiple freeze-thaw processes. Practice has shown that the more freeze-thaw times the biological sample sampling undergoes, the greater the impact on its test results.

[0003] In addition, the existing biological sample freezing and sampling and testing operations have many disadvantages, especially because the opening of the cryopreservation tube used is very small, the operation of loading the biological sample into the cryopreservation tube is very troublesome, and it must be operated with the help of specific tools, and it takes a long time. More importantly, whether it is sampling from the cup body for freezing or sampling and testing after freezing and thawing, the existing methods cannot be used to quickly, accurately, and smoothly perform sampling, and there is no sophisticated instrument for sampling from the cup body for freezing. Taking fecal sampling and freezing as an example, it takes 1-2 minutes to load 2 / 3 of the biological sample (feces) in each cryopreservation tube. Summary of the invention

[0004] At least in view of the technical problems mentioned in the background technology, the present invention aims to provide a biological sample sampling instrument and a biological sample freezing tube.

[0005] The present invention adopts the following technical solution.

[0006] The present invention provides a biological sample sampling instrument, which includes a barrel cover, an inner tube with an open portion is axially arranged on the barrel cover, the lower end of the open portion and the lower end of the inner tube are located in the same radial section, the inner cavity of the inner tube serves as a space for accommodating biological samples, and the outer wall of the inner tube is provided with scale marks, wherein the barrel cover serves as a gripping portion for the operator to hold. With such a scheme, the sampling of biological samples (taking feces as an example) can be achieved within five seconds, which completely subverts the traditional sampling method. The operation process is: after holding the barrel cover, the inner tube is inserted into the biological sample in the cup body, and then pulled out. At this time, the biological sample smoothly enters the inner cavity of the inner tube, and then the residue on the outer wall of the inner tube is wiped off, which cleverly achieves fast, accurate and smooth sampling of biological samples. The present invention also provides a biological sample cryopreservation tube, which uses the aforementioned biological sample sampling instrument, and specifically includes a cylinder with a closed bottom and an open top, a cylinder cover is arranged on the top of the cylinder, an inner tube with an open portion is axially arranged on the cylinder cover, the lower end of the open portion and the lower end of the inner tube are located in the same radial cross section, and the inner cavity of the inner tube is used as a space for accommodating biological samples. By adopting such a solution, the freeze-thaw process in the existing method is omitted, at least fundamentally solving the problem of affecting the accuracy of biological sample detection results due to the freeze-thaw process, and at the same time greatly simplifying the operation process of loading biological samples.

[0007] Preferably, in the first solution, when the cylinder cover is fitted on the top of the cylinder, the lower end of the inner tube just abuts against the bottom wall of the cylinder.

[0008] Preferably, in the second solution, when the cylinder cover is fitted on the top of the cylinder body, the lower end of the inner tube is suspended in the air, and the lower part of the inner tube is fitted and connected with the end cover.

[0009] Furthermore, when the cylinder cover is fitted on the top of the cylinder body, the outer wall of the inner tube is against or close to the inner wall of the cylinder cover.

[0010] Furthermore, the upper end of the open portion is close to the cylinder cover.

[0011] Preferably, in the third solution, the cylinder cover is replaced by a plug element.

[0012] Furthermore, the inner tube is provided with scale markings.

[0013] Furthermore, the outer wall of the inner tube is in an arc shape.

[0014] Furthermore, detachable partitions are radially arranged on the inner tube, and the space between adjacent partitions in the inner cavity of the inner tube is used to accommodate quantitative biological samples.

[0015] Furthermore, the inner tube is provided with an annular groove for facilitating cutting or breaking.

[0016] Beneficial effects: The scheme of the present invention eliminates the freeze-thaw process in the existing method, which not only fundamentally solves the problem that the freeze-thaw process affects the accuracy of the biological sample detection results, but also greatly simplifies the operation process of loading biological samples. In the process of loading biological samples, there is no need to use other specific tools (such as spoons, tweezers), because the inner tube with an open part itself is both a sample loading tool and has the function of freezing samples. It also significantly shortens the time for loading biological samples and sampling (taking out biological samples from the freezing tube). The time for loading biological samples is shortened from more than one minute to less than ten seconds, and the time for taking out biological samples is shortened from no less than two minutes to less than twenty seconds. Sampling can also be performed accurately and smoothly. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic diagram of the external structure of the biological sample cryopreservation tube in Example 1;

[0018] Figure 2 This is a schematic diagram of the inner tube and end cap structure of the biological sample freezing tube in Example 1. This structure can also be used independently as a biological sample sampling instrument;

[0019] Figure 3 is a schematic diagram of a radial cross section of the biological sample cryopreservation tube in Example 1;

[0020] Figure 4 is a cross-sectional schematic diagram of the biological sample cryopreservation tube in Example 1;

[0021] Figure 5 is a cross-sectional schematic diagram of the biological sample cryopreservation tube in Example 2;

[0022] Figure 6 Schematic diagram of the lower end of the inner tube of the biological sample freezing tube in Example 2;

[0023] Figure 7 is a cross-sectional schematic diagram of the biological sample cryopreservation tube in Example 3;

[0024] Figure 8 Schematic diagram of the upper end of the inner tube of the biological sample freezing tube in Example 3;

[0025] Fig. 9 is a cross-sectional schematic diagram of the biological sample cryopreservation tube in Example 4;

[0026] Fig.10 This is a schematic diagram of the structure of the inner tube and end cap (plug element) of the biological sample freezing tube in Example 4. This structure can also be used independently as a biological sample sampling instrument;

[0027] Fig.11 This is a schematic diagram of the structure of the inner tube and end cap (plug element) of the biological sample freezing tube in Example 5. This structure can also be used independently as a biological sample sampling instrument;

[0028] Fig.12 Schematic diagram of the structure of the inner tube and end cap (plug element) of the biological sample freezing tube in Example 6. DETAILED DESCRIPTION

[0029] The technical solution of the present invention is described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] Example 1: Combination Figures 1 to 4 As shown, a biological sample freezing tube comprises a cylinder 1 with a closed bottom and an open top, a cylinder cover 2 is arranged on the top of the cylinder 1, an inner tube 3 with an open portion 4 is axially fixedly arranged on the cylinder cover 2, the lower end of the open portion 4 and the lower end of the inner tube 3 are located in the same radial section, the upper end of the open portion 4 is close to the cylinder cover 2, and the inner cavity of the inner tube 3 is used as a space for accommodating biological samples. When the cylinder cover 2 is matched with the top of the cylinder 1, the lower end of the inner tube 3 just abuts against the bottom wall of the cylinder 1 (combined with the cylinder cover 2). Figure 4 As shown, the outer wall of the inner tube 3 is close to or close to the inner wall of the cylinder cover 2 (combined with Figure 3 As shown, the inner tube 3 is provided with a scale mark 8 (combined with Figure 2 As shown in FIG. 1 ), the outer wall of the inner tube 3 is in an arc shape.

[0031] The operation method of loading biological samples is as follows: hold the tube cover 2, insert the inner tube 3 into the biological sample (taking feces as an example, the feces at this time refers to the fresh feces collected and loaded into the cup body or box body), and then pull out the inner tube 3 (this action process is similar to the operation process of shoveling soil with a shovel, and its application in the field of biological sample preservation in the present invention is very clever), and the biological sample will be automatically loaded into the inner cavity of the inner tube 3, and then use medical cotton cloth or paper towel to wipe off the biological sample on the outer wall of the inner tube 3, and then insert the inner tube 3 into the cylinder body 1 and tighten the tube cover 2. The above-mentioned whole process takes less than 10 seconds, and finally it is frozen. However, the conventional operation method described in the background technology requires the use of a specific tool (spoon) to transfer it little by little, and it takes 1-2 minutes to load 2 / 3 of the biological sample in the inner cavity of each cryopreservation tube.

[0032] Sampling operation method: After loosening the tube cover 2, take out the inner tube 3 from the cylinder body 1, and then cut / cut the inner tube 3 according to the amount of biological samples required for the test (if the total length of the biological sample in the inner tube 3 is 6cm, and only 2cm of length is required for this test, then the biological sample of the corresponding size can be cut according to the scale mark 8, and cut together with the inner tube 3). The above-mentioned whole process takes less than 20 seconds, eliminating the thawing process, and then the remaining biological samples are frozen (the remaining inner tube 3 and its biological samples are reinserted into the cylinder body 1 and the tube cover 2 is tightened to continue freezing). However, the conventional operation method described in the background technology requires thawing first (a single thawing takes one and a half minutes), and then taking out from the inner cavity of the cryopreservation tube with a spoon in multiple times, and each sampling takes at least two minutes.

[0033] Example 2: Combination Figure 1 , Figure 5 and Figure 6 As shown, a biological sample freezing tube includes a cylinder 1 with a closed bottom and an open top, a cylinder cover 2 is arranged on the top of the cylinder 1, an inner tube 3 with an open portion 4 is axially fixedly arranged on the cylinder cover 2, the lower end of the open portion 4 and the lower end of the inner tube 3 are located in the same radial section, the upper end of the open portion 4 is close to the cylinder cover 2, and the inner cavity of the inner tube 3 is used as a space for accommodating biological samples. When the cylinder cover 2 is matched with the top of the cylinder 1, the lower end of the inner tube 3 is suspended, and the lower part of the inner tube 3 is matched and connected with the end cover 5 (such as Figure 5 and Figure 6 As shown in FIG. 1 ), the end cover 5 can be flexibly disassembled, and the outer wall of the inner tube 3 is close to or close to the inner wall of the cylinder cover 2 (see FIG. 1 ). Figure 3 As shown in the figure, the inner tube 3 is provided with a scale mark 8, and the outer wall of the inner tube 3 is in an arc shape. The operation method in this embodiment refers to the embodiment, and the operation process is different from that in that the end cap 5 needs to be removed before loading the biological sample, and the end cap 5 needs to be covered before loading the biological sample.

[0034] Example 3: A biological sample cryopreservation tube, referring to Example 1, the main difference between the above-mentioned embodiment and Example 1 is that: Figure 7 and Figure 8 As shown, the inner tube 3 is connected to the cylinder cover 2 by threads, and the threaded portion 9 is located on the boss of the cylinder cover 2. Such a structure makes it easier to replace the inner tube 3.

[0035] Example 4: A biological sample cryopreservation tube, referring to Example 1, the main difference between the above-mentioned embodiment and Example 1 is that: Fig. 9 and Fig.10 As shown, the cylinder cover 2 is replaced by a plug element (similar to the plug of a bottle stopper), and the upper end of the inner tube 3 is inserted on the cylinder cover 2. This structure makes it easier to insert the cylinder cover 2 and the inner tube 3 into the cylinder body 1 and also easier to remove.

[0036] Example 5: A biological sample cryopreservation tube, referring to Example 1, the main difference between the above-mentioned embodiment and Example 1 is that: Figures 9 to 11 As shown, the cylinder cover 2 is replaced by a plug element (similar to the plug of a bottle stopper), the upper end of the inner tube 3 is inserted on the cylinder cover 2, and the inner tube 3 is provided with an annular groove 7 for easy cutting or breaking.

[0037] Example 6: A biological sample cryopreservation tube, referring to Example 1, the main difference between the above-mentioned embodiment and Example 1 is that: Fig.12 As shown, the barrel cover 2 is replaced by a plug element (similar to the plug of a bottle stopper), the upper end of the inner tube 3 is inserted on the barrel cover 2, and a detachable partition 6 is radially arranged on the inner tube 3. The space between adjacent partitions 6 in the inner cavity of the inner tube 3 is used to accommodate quantitative biological samples, that is, the space between adjacent partitions 6 is pre-set. When in use, the inner tube 3 is inserted into the biological sample and then the inner tube 3 is pulled out, and the biological sample will be automatically loaded into the inner cavity of the inner tube 3, and then the partition 6 is inserted, and then the biological sample on the outer wall of the inner tube 3 is wiped off with medical cotton cloth or paper towel; when sampling is required, the partition 6 is first taken out, and then the inner tube 3 is cut off / sheared from the insertion position of the partition 6.

[0038] The use of the biological sample cryopreservation tube of the present invention eliminates the freeze-thaw process in the existing method, which not only fundamentally solves the problem of affecting the accuracy of biological sample detection results due to the freeze-thaw process, but also greatly simplifies the operation process of loading biological samples. In the process of loading biological samples, there is no need to use other specific tools (such as spoons and tweezers) because the inner tube with an open portion itself is both a sample loading tool and has the function of freezing samples. It also significantly shortens the time for loading biological samples and sampling (taking out biological samples from the cryopreservation tube). The time for loading biological samples is shortened from more than one minute to less than ten seconds, and the time for taking out biological samples is shortened from no less than two minutes to less than twenty seconds. Sampling can also be performed accurately and smoothly.

[0039] Embodiment 7: A biological sample sampling instrument, such as Figure 2 As shown, it consists of a tube cover 2 and an inner tube 3. An inner tube 3 with an open portion 4 is axially arranged on the tube cover 2 (it can be obtained by axially cutting off a part of a circular tube). The lower end of the open portion 4 and the lower end of the inner tube 3 are located in the same radial section, and the inner cavity of the inner tube 3 serves as a space for accommodating biological samples. The outer wall of the inner tube 3 is provided with scale marks 8, wherein the tube cover 2 serves as a grip for the operator to hold. With such a scheme, the sampling of biological samples (taking feces as an example) can be achieved within five seconds, which completely subverts the traditional sampling method. The operation process is: after holding the tube cover 2, the inner tube 3 is inserted into the biological sample in the cup body, and then pulled out. At this time, the biological sample has smoothly entered the inner cavity of the inner tube 3, and then the residue on the outer wall of the inner tube 3 is wiped off.

[0040] Embodiment 8: A biological sample sampling instrument, such as Fig.10As shown, it is composed of a cylinder cover 2 and an inner tube 3. The cylinder cover 2 adopts a cylindrical plug structure. The inner tube 3 with an open portion 4 is axially arranged on the cylinder cover 2 (it can be obtained by axially cutting off a part of a circular tube). The lower end of the open portion 4 and the lower end of the inner tube 3 are located in the same radial section, and the inner cavity of the inner tube 3 is used as a space for accommodating biological samples. The outer wall of the inner tube 3 is provided with a scale mark 8, wherein the cylinder cover 2 is used as a grip for the operator to hold, and the operation process is the same as that of Example 7.

Claims

1. A biological sample cryopreservation tube, comprising a cylinder (1) with a closed bottom and an open top, a cylinder cover (2) disposed on the top of the cylinder (1), characterized in that: An inner tube (3) with an open portion (4) is axially arranged on the tube cover (2), the upper end of the open portion (4) is close to the tube cover (2), the lower end of the open portion (4) and the lower end of the inner tube (3) are located in the same radial cross section, the inner cavity of the inner tube (3) serves as a space for accommodating biological samples, and the outer wall of the inner tube (3) is in an arc shape; detachable partitions (6) are radially arranged on the inner tube (3), and the space between adjacent partitions (6) in the inner cavity of the inner tube (3) is used to accommodate quantitative biological samples; When the cylinder cover (2) is fitted on the top of the cylinder body (1), the lower end of the inner tube (3) just abuts against the bottom wall of the cylinder body (1), and the outer wall of the inner tube (3) abuts against or is close to the inner wall of the cylinder cover (2); or, when the cylinder cover (2) is fitted on the top of the cylinder body (1), the lower end of the inner tube (3) is suspended in the air, the lower part of the inner tube (3) is fitted with an end cover (5), and the outer wall of the inner tube (3) abuts against or is close to the inner wall of the cylinder cover (2); After the inner tube (3) is inserted into the biological sample, the inner tube (3) is pulled out, and the biological sample is automatically loaded into the inner cavity of the inner tube (3), and then the partition (6) is inserted and installed, and then the biological sample on the outer wall of the inner tube (3) is wiped off with medical cotton cloth or paper towel; when sampling is required, the partition (6) is first removed, and then the inner tube (3) is cut off or sheared from the insertion position of the partition (6); In the process of loading biological samples, there is no need to use a spoon or tweezers, and the inner tube (3) with the open portion (4) itself is both a sample loading tool and has the function of freezing the sample.

2. The biological sample cryopreservation tube according to claim 1, characterized in that: The cylinder cover (2) is replaced by a plug element.

3. The biological sample cryopreservation tube according to claim 1, characterized in that: The inner tube (3) is provided with a scale mark (8).

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