A poplar cutting slow bud and root promotion seedling raising method

Abstract

The present invention belongs to the technical field of slow sprouting of poplars, and specifically relates to a method for slow sprouting and rooting promotion of poplar cuttings. The present invention provides any of the following uses of Qingxiansu: 1) delaying the germination of the terminal buds of poplar cuttings; 2) promoting the growth of the root system of poplar cuttings; 3) inhibiting the premature germination of the terminal buds of poplar cuttings, using the nutrients stored in the poplar cuttings for rooting, and promoting the growth of the root system of the poplar cuttings. The method for slow sprouting and rooting promotion of poplar cuttings of the present invention comprises the step of treating the poplar cuttings with the Qingxiansu, specifically: after the upper part of the poplar cuttings is cut flat, it is immersed in a Qingxiansu solution with a concentration of 1000mg / kg to 1200mg / kg for 2s to 4s. The method of the present invention combines the comprehensive measures of slow sprouting with Qingxiansu, dipping roots in rooting powder, raising seedlings on high ridges, adding soil conditioners to the soil, and covering the ground with film, which can not only adjust the time difference between sprouting and rooting to maximize the nutrient utilization of cuttings, but also promote root growth. A strong root system can also promote the growth of subsequent seedlings.

Description

Technical Field

[0001] The invention belongs to the technical field of poplar bud slowing, and in particular relates to a poplar cutting bud slowing rooting promotion seedling raising method. Background Art

[0002] Prior art Poplar hardwood cuttings are carried out in mid-March, and the early root growth is slow after cutting, and budding and rooting consume cutting nutrition at the same time, and there is a relationship between rooting and budding competing for cutting nutrition in the early stage. And in March and April, the north is prone to late spring cold, and the growth of the cutting terminal bud is often hindered by the late spring cold after germination, and there is a similar effect of topping. When the temperature rises, the bud growth rate and budding quality of the second sequence will be better than the terminal bud. But the cutting seedlings often retain only one bud that grows better, and the general terminal bud growth has apical dominance, which will erase the two buds, but because of the damage of late spring cold, the growth of the terminal bud is damaged and sometimes chooses to leave the two buds that grow more vigorously, so that the part of nutrition used for the growth of the terminal bud in the early stage is a great waste of cutting nutrition. Therefore, a kind of regulating technology is needed to use this part of nutrition only for early rooting, so that the bud germination is later, so that the cutting nutrition utilization can be maximized, and the root growth can be promoted again, and the robust root system can promote the growth of subsequent seedlings. Summary of the invention

[0003] In order to fill the gap in the prior art, the present invention provides a poplar cutting slow bud and root promotion seedling raising method.

[0004] The primary purpose of the present invention is to provide any of the following uses of cyanocbine:

[0005] 1) Delay the germination of terminal buds of poplar cuttings;

[0006] 2) Promote the root growth of poplar cuttings;

[0007] 3) Inhibit the premature germination of the top buds of poplar cuttings, use the nutrients stored in the poplar cuttings for rooting, and promote the growth of the root system of poplar cuttings.

[0008] Furthermore, the poplar varieties include European and American poplar and Populus cathayana.

[0009] Another object of the present invention is to provide a method for raising poplar cuttings by slowing budding and promoting rooting, comprising the step of treating poplar cuttings with the green fresh extract, specifically: cutting the upper part of the poplar cuttings flat, and immersing them in a green fresh extract solution with a concentration of 1000mg / kg to 1200mg / kg for 2s to 4s.

[0010] Furthermore, in the method, the flat cut is 0.8 cm to 1.5 cm away from the terminal bud.

[0011] Furthermore, in the method, the upper 5 cm to 8 cm of the poplar cuttings are immersed in the green fresh element solution.

[0012] Furthermore, the method also includes the step of treating the poplar cuttings with an ABT rooting powder solution after treating the poplar cuttings with Qingxiansu, specifically: after obliquely cutting the lower part of the poplar cuttings, immersing them in an ABT rooting powder solution with a concentration of 40 to 60 mg / kg for 30s to 60s.

[0013] Furthermore, in the method, the lower 3 cm to 5 cm of the poplar cuttings are immersed in the ABT rooting powder solution.

[0014] Furthermore, in the method, a robust stem with full buds, no pests and diseases, and a high degree of lignification is selected as a seed strip, which is cut into cuttings with a length of 15 cm to 20 cm, ensuring that there are 2 to 3 full buds on the cuttings.

[0015] Furthermore, in the method, after the cuttings survive, when the seedlings are 20 cm tall, a strong sprout is retained as the main trunk, and the remaining sprouts are removed from the base. After the trunk is fixed, buds are removed regularly and in a timely manner until the seedlings are 2 m tall.

[0016] Furthermore, the poplar varieties include European and American poplar and Populus cathayana.

[0017] Beneficial effects:

[0018] The present invention inhibits premature germination of the terminal buds of poplar cuttings seedlings by using cyanogen in the early stage of cuttings, uses the nutrients stored in the early cuttings for rooting, and increases the growth amount and rooting speed of fibrous roots by combining comprehensive measures such as heating of mulch film, rooting powder, soil finishing agent, etc. When the temperature reaches a certain accumulation, the terminal buds can grow rapidly. The survival rate of the cuttings after adjustment is improved, and the quality of the seedlings is improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 The plant types of poplar cuttings treated with the A2B3 combination (A), the A1B3 combination (B) and the clear water control (C) in Example 3 are compared. DETAILED DESCRIPTION

[0020] The present invention is described in detail below in conjunction with the accompanying drawings and specific examples, but should not be construed as limiting the present invention. Unless otherwise specified, the technical means used in the following examples are conventional means well known to those skilled in the art, and the materials, reagents, etc. used in the following examples, unless otherwise specified, can be obtained from commercial sources.

[0021] The high nitrogen and high phosphorus compound fertilizer in the following examples is a authentic balanced high nitrogen, high phosphorus and high potassium nitrogen, phosphorus and potassium compound fertilizer for flushing with Dugao macronutrient water-soluble fertilizer.

[0022] The chlorpyrifos is Dizhonglang brand chlorpyrifos, the active ingredient and its content: 10% chlorpyrifos, dosage form: granules.

[0023] The soil conditioner was Agri-star soil loosening and root promotion agent, which was purchased from Henan Locomotive Agricultural Technology Co., Ltd.

[0024] Green fresh extract was purchased from Shanghai Yien Chemical Technology Co., Ltd.

[0025] ABT rooting powder was purchased from Beijing Abidi Biotechnology Co., Ltd., with a total active ingredient content of 50%, acetic acid content of 20%, and indoleacetic acid content of 30%.

[0026] Example 1: A method for raising poplar seedlings by slow budding and rooting

[0027] S1. Land preparation

[0028] Deeply till the soil to more than 40cm, and apply high nitrogen and high phosphorus compound fertilizer 50kg / 667m 3 , irrigate thoroughly, dry in the sun for 10 days, harrow the nursery ground, and mix the soil with 5% zinc sulfate and phosphorus 3kg / 667m 3 To control underground pests.

[0029] S2. Ridge making

[0030] The seedlings were grown on high ridges, with a ridge bottom width of 70 cm, a ridge height of 20 cm, and a ridge spacing of 60 cm. When raising ridges, apply soil conditioner (Agri-star) at a rate of 2 kg / 667 m 3 Before cutting, water the nursery thoroughly once, wait for the nursery to dry in the shade, cover the ridge surface with black mulch, and compact the soil on both sides.

[0031] S3. Cuttings processing

[0032] Select one-year-old one-stem seedlings with strong growth, full buds, no diseases and insect pests, and high degree of lignification, cut off the base and tip of the stem, cut the middle part of the stem into cuttings with a length of 18 cm, and ensure that there are 2 to 3 full buds on the cuttings;

[0033] Cut the top of the cutting flat, with the incision 0.8cm to 1.5cm away from the terminal bud; cut the bottom obliquely into a horseshoe shape. Before cutting, bundle the cuttings of uniform thickness and soak them in clean water for more than 48 hours. After rinsing and draining, first dip the top 5cm to 8cm of the cuttings into a solution of 800 to 2000mg / kg of Qingxiansu for 3s, then dip the bottom 3cm to 5cm of the cuttings into a solution of 50mg / kg of ABT rooting powder for 40s, and take out the cuttings for use.

[0034] S4, cuttings

[0035] Plant cuttings on the ridges with a spacing of 25 cm between them, compact the soil so that the soil and cuttings are tightly combined, and then water them thoroughly.

[0036] S5. After the cuttings survive, when the seedlings are 20 cm tall, keep a strong sprout as the main trunk, and remove the other sprouts from the base. After the trunk is fixed, remove the buds regularly and in time until the seedlings are 2 m tall.

[0037] S6. Water the plants in time after cutting according to soil moisture conditions.

[0038] Experiment 1: In order to test the optimal concentration of Qingxiansu for its slow sprouting effect, a single factor experiment of Qingxiansu was set up

[0039] The method of Example 1 was applied to Populus euphorbia to illustrate the effect of the present invention. Cuttings were carried out on March 14, 2022. The number of cuttings in each group was the same, 90 plants per group, and the random block design was repeated 3 times, with 30 plants in each repeat. After cutting, the germination time of the cutting seedlings in different groups was investigated.

[0040] The results are shown in Table 1. Compared with the group without adding cyanocobalamin (7 groups, clean water), when the cyanocobalamin concentration was 800-1800 mg / kg, it could delay the germination time of the terminal buds of European and American Poplar cuttings (i.e., the delayed germination effect). However, when the cyanocobalamin concentration was 800 mg / kg, the delayed germination effect was not obvious; when the cyanocobalamin concentration was 1500 mg / kg, although it could significantly delay the germination time of the terminal buds of European and American Poplar cuttings, a small number of terminal buds did not germinate at the same time; when the cyanocobalamin concentration was 2000 mg / kg, a large number of terminal buds did not germinate; when the cyanocobalamin concentration was 1000-1200 mg / kg, it could not only delay the germination time of the terminal buds of European and American Poplar cuttings, but also make all the terminal buds germinate. Therefore, the optimal concentration of cyanocobalamin was determined to be 1000-1200 mg / kg.

[0041] Table 1 The slow sprouting effect of different concentrations of Qingxiansu

[0042] Group Green fresh element concentration (mg / kg) The first terminal bud sprouting time All terminal buds germination time 1 group 800 March 28 March 30 2 groups 1000 April 4 April 9 3 groups 1200 April 6 April 10 4 groups 1500 April 8 On April 15, a small number of terminal buds did not germinate 5 groups 1800 April 7 On April 18, most of the terminal buds did not germinate 6 groups 2000 Almost all terminal buds fail to germinate __ 7 groups 0 March 25 March 28

[0043] Embodiment 2: A method for raising poplar seedlings by slow budding and rooting

[0044] S1. Land preparation

[0045] Deeply till the soil to more than 40cm, and apply high nitrogen and high phosphorus compound fertilizer 50kg / 667m 3 , irrigate thoroughly, dry in the sun for 10 days, harrow the nursery ground, and mix the soil with 5% zinc sulfate and phosphorus 3kg / 667m 3 To control underground pests.

[0046] S2. Ridge making

[0047] The seedlings were grown on high ridges, with a ridge bottom width of 70 cm, a ridge height of 20 cm, and a ridge spacing of 60 cm. When raising ridges, apply soil conditioner (Agri-star) at a rate of 2 kg / 667 m 3Before cutting, water the nursery thoroughly once, wait for the nursery to dry in the shade, cover the ridge surface with black mulch, and compact the soil on both sides.

[0048] S3. Cuttings processing

[0049] Select one-year-old one-stem seedlings with strong growth, full buds, no diseases and insect pests, and high degree of lignification, cut off the base and tip of the stem, cut the middle part of the stem into cuttings with a length of 18 cm, and ensure that there are 2 to 3 full buds on the cuttings;

[0050] Cut the top of the cutting flat, with the incision 0.8cm to 1.5cm away from the terminal bud; cut the bottom obliquely into a horseshoe shape. Before cutting, bundle the cuttings of uniform thickness and soak them in clean water for more than 48 hours. After rinsing and draining, first dip the top 5cm to 8cm of the cuttings in a 1200mg / kg Qingxiansu solution for 3s, then dip the bottom 3cm to 5cm of the cuttings in a 50-150mg / kg ABT rooting powder solution for 20-40s, and take out the cuttings for later use.

[0051] S4, cuttings

[0052] Plant cuttings on the ridges with a spacing of 25 cm between them, compact the soil so that the soil and cuttings are tightly combined, and then water them thoroughly.

[0053] S5. After the cuttings survive, when the seedlings are 20 cm tall, keep a strong sprout as the main trunk, and remove the other sprouts from the base. After the trunk is fixed, remove the buds regularly and in time until the seedlings are 2 m tall.

[0054] S6. Water the plants in time after cutting according to soil moisture conditions.

[0055] Experiment 2: In order to test the best concentration and method of use of rooting powder to promote rooting, a single-factor experiment of rooting powder was set up

[0056] The method of Example 2 was applied to Populus occidentalis to illustrate the effect of the present invention. Cuttings were carried out on March 14, 2022. The number of cuttings in each group was the same, 90 plants per group, and the random block design was repeated 3 times, with 30 plants in each repeat. The rooting rate, survival rate and root characteristics of different groups were measured 60 days after cutting.

[0057] The results are shown in Table 2. When the rooting powder concentration is 50 mg / kg, the soaking time is 40 s, the rooting powder concentration is 100 mg / kg, the soaking time is 20 s, and the rooting powder concentration is 100 mg / kg, and the soaking time is 40 s, the rooting effect of European and American Poplar is better.

[0058] Table 2 Root-promoting effect of rooting powder at different concentrations

[0059]

[0060] Embodiment 3: A method for raising poplar seedlings by slow budding and rooting

[0061] S1. Land preparation

[0062] Deeply till the soil to more than 40cm, and apply high nitrogen and high phosphorus compound fertilizer 50kg / 667m 3 , irrigate thoroughly, dry in the sun for 10 days, harrow the nursery ground, and mix the soil with 5% zinc sulfate and phosphorus 3kg / 667m 3 To control underground pests.

[0063] S2. Ridge making

[0064] The seedlings were grown on high ridges, with a ridge bottom width of 70 cm, a ridge height of 20 cm, and a ridge spacing of 60 cm. When raising ridges, apply soil conditioner (Agri-star) at a rate of 2 kg / 667 m 3 Before cutting, water the nursery thoroughly once, wait for the nursery to dry in the shade, cover the ridge surface with black mulch, and compact the soil on both sides.

[0065] S3. Cuttings processing

[0066] Select one-year-old one-stem seedlings with strong growth, full buds, no diseases and insect pests, and high degree of lignification, cut off the base and tip of the stem, cut the middle part of the stem into cuttings with a length of 18 cm, and ensure that there are 2 to 3 full buds on the cuttings;

[0067] Cut the top of the cutting flat, with the incision 0.8cm to 1.5cm away from the terminal bud; cut the bottom obliquely into a horseshoe shape. Before cutting, bundle the cuttings of uniform thickness and soak them in clean water for more than 48 hours. After rinsing and drying, first dip the top 5cm to 8cm of the cuttings into a 1000mg / kg or 1200mg / kg Qingxiansu solution for 3s, then dip the bottom 3cm to 5cm of the cuttings into a 50mg / kg ABT rooting powder solution for 40s, or dip the bottom 3cm to 5cm of the cuttings into a 100mg / kg ABT rooting powder solution for 20s, or dip the bottom 3cm to 5cm of the cuttings into a 100mg / kg ABT rooting powder solution for 40s, and then take out the cuttings for use.

[0068] S4, cuttings

[0069] Plant cuttings on the ridges with a spacing of 25 cm between them, compact the soil so that the soil and cuttings are tightly combined, and then water them thoroughly.

[0070] S5. After the cuttings survive, when the seedlings are 20 cm tall, keep a strong sprout as the main trunk, and remove the other sprouts from the base. After the trunk is fixed, remove the buds regularly and in time until the seedlings are 2 m tall.

[0071] S6. Water the plants in time after cutting according to soil moisture conditions.

[0072] Experiment 3: Two-factor three-level orthogonal experiment

[0073] Qingxiansu 1000mg / kg (A1), 1200mg / kg (A2); rooting powder 100mg / kg soaked for 40s (B1), 100mg / kg soaked for 20s (B2), 50mg / kg soaked for 40s (B3). Cuttings were carried out on March 15, 2022. The number of cuttings in each group was the same. There were 6 treatments in the orthogonal experiment, 30 seedlings in each treatment, and 3 replicates. The seedling height, number and length of primary roots, and the amount of fibrous roots of different groups were measured 70 days after cutting.

[0074] The results are shown in Table 3 and Figure 1 As shown, the A1B3 and A2B3 combinations had the best comprehensive effects on seedling height, primary taproot number, length, and fibrous root quantity. The primary root length of the A2B1 and A2B2 combinations was shorter than that of the water control, indicating that the higher the concentration of rooting powder, the better the effect of combining with 1200 mg / kg Qingxiansu solution.

[0075] Table 3 Orthogonal experiment results

[0076] Group Factor combination Seedling height / cm Number of primary roots Primary root length / cm Root volume 1 group A1B1 29.67 13.71 12.63 less 2 groups A1B2 31.14 16.14 12.32 More 3 groups A1B3 32.54 19.64 14.61 many 4 groups A2B1 28.24 14.85 9.85 few 5 groups A2B2 26.22 14.50 10.87 less 6 groups A2B3 31.47 20.65 13.33 many 7 groups Clear water control 25.38 12.39 11.24 few

[0077] It should be noted that when the claims of the present invention involve numerical ranges, it should be understood that the two endpoints of each numerical range and any numerical value between the two endpoints can be selected. In order to avoid redundancy, the present invention describes a preferred embodiment.

[0078] Although the preferred embodiments of the present invention have been described, those skilled in the art may make other changes and modifications to these embodiments once they have learned the basic creative concept. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention.

[0079] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. Any of the following uses of Qingxiansu: 1) Delay the germination of terminal buds of poplar cuttings; 2) Promote the root growth of poplar cuttings; 3) Inhibit premature germination of the top buds of poplar cuttings, use the nutrients stored in the poplar cuttings for rooting, and promote the growth of the root system of poplar cuttings; Applications include: During the poplar cutting seedling raising process, the poplar cuttings are treated with cyanogenetic acid. Specifically, the upper part of the poplar cuttings is cut flat and then immersed in a cyanogenetic acid solution with a concentration of 1000 mg / kg~1200 mg / kg for 2 s~4 s.

2. The use according to claim 1, characterized in that The poplar varieties include European and American poplar and Populus cathayana.

3. The use according to claim 2, characterized in that: The horizontal incision should be 0.8 cm to 1.5 cm away from the terminal bud.

4. The use according to claim 3, characterized in that Immerse the upper 5 cm to 8 cm of the poplar cuttings into the green fresh element solution.

5. The use according to claim 4, characterized in that The method also includes the step of treating the poplar cuttings with an ABT rooting powder solution after treating the poplar cuttings with Qingxiansu. Specifically, the lower part of the poplar cuttings is cut obliquely, and then immersed in an ABT rooting powder solution with a concentration of 40-60 mg / kg for 30 s-60 s.

6. The use according to claim 5, characterized in that Immerse the lower 3 cm to 5 cm of the poplar cuttings into the ABT rooting powder solution.

7. The use according to claim 6, characterized in that Select strong stems with full buds, no diseases or insect pests, and high degree of lignification as seed strips. Cut them into cuttings with a length of 15 cm to 20 cm, and ensure that there are 2 to 3 full buds on the cuttings.

8. The use according to claim 7, characterized in that After the cuttings survive, when the seedlings are 20 cm tall, retain a strong sprout as the main trunk, and remove the rest from the base. After the trunk is fixed, remove the buds regularly and in time until the seedlings are 2 m tall.

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