Chitosan oligosaccharide enzymolysis device suitable for preparing chitosan oligosaccharides

Abstract

The present invention relates to the technical field of chitosan oligosaccharide preparation, and specifically, to a chitosan oligosaccharide enzymolysis device suitable for chitosan oligosaccharide preparation, comprising an enzymolysis tank and a reaction barrel, wherein a stirring assembly for uniformly mixing reactants is provided in the reaction barrel, and the stirring assembly comprises a rotating shaft arranged in the middle of the reaction barrel, a driving structure for driving the rotating shaft to rotate, and two stirring rod structures staggered up and down. The device can rotate the stirring blade in the vertical direction during the stirring process by arranging the stirring rod structure, so as to avoid the reaction liquid from vortexing under the action of the stirring rod during stirring, and the reaction liquid on the outside and inside is heated unevenly, which affects the quality and rate of enzymolysis; by arranging the air outlet structure and the air pipe structure, not only can the oxygen in the tank body be discharged to avoid non-enzymatic browning, but the rapidly ejected airflow can also drive the reaction liquid at the bottom to churn rapidly, so that the reaction liquid is heated more evenly and the quality of enzymolysis is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of chitosan oligosaccharide preparation, and in particular relates to a chitosan oligosaccharide enzymolysis device suitable for chitosan oligosaccharide preparation. Background Art

[0002] Chitosan oligosaccharide is an oligosaccharide product with a degree of polymerization between 2 and 20. Due to its low degree of polymerization, it has the advantages of high solubility, full solubility in water and easy absorption. It is generally prepared by enzymatic hydrolysis of chitosan.

[0003] For example, the invention patent with the authorization announcement number CN118599649A in the field of chitosan oligosaccharide preparation technology discloses a temperature-controllable enzymolysis device for preventing the precipitation of chitosan oligosaccharide, including a chassis, a fixing assembly is provided at the bottom of the chassis, a control assembly is provided at the upper part of the chassis, a support assembly is provided at the upper part of the chassis, the support assembly is arranged on one side of the control assembly, a reaction tank is provided at the upper part of the support assembly, a stirring assembly is provided at the top of the reaction tank, an auxiliary assembly is provided at the bottom of the reaction tank, a filling assembly is provided on one side of the reaction tank, and the filling assembly is connected to the reaction tank. The present invention reassembles and integrates multiple components, abandons traditional physical and chemical preparation devices, adopts a professional enzymatic degradation method, can stir the raw materials to increase the efficiency of the enzymatic degradation, and can monitor the internal and external temperatures of the reaction tank in real time to ensure the efficiency of the enzymatic degradation, and also prevents the reaction tank from being overheated and injuring the staff, thereby expanding the scope of use of the present invention.

[0004] Combining the above cases and actual conditions, we found the following problems: non-enzymatic browning may occur during the enzymatic hydrolysis of chitosan, that is, the complex reaction between the amino group and the reducing sugar in the chitosan oligosaccharide will eventually generate colored substances and a series of flavor substances, affecting the preparation quality of chitosan oligosaccharide. This is related to the pH value of the reaction solution, the reaction temperature and the oxygen content of the reaction environment. However, the current enzymatic hydrolysis device generally only controls the pH value and reaction temperature during the reaction process, and it is difficult to solve the oxygen problem. At the same time, the existing enzymatic hydrolysis device uses a stirring rod for stirring. During the stirring process, it will drive the reaction liquid to rotate to form a vortex, which makes it difficult for the reaction liquid to quickly exchange positions between the middle and the outside. During the water bath heating process, it will cause uneven heating inside and outside the reaction liquid, affecting the quality of the enzymatic hydrolysis. Summary of the invention

[0005] The purpose of the present invention is to provide a chitosan oligosaccharide enzymolysis device suitable for preparing chitosan oligosaccharides, so as to solve the above problems of the prior art.

[0006] To achieve the above-mentioned object, the present invention provides a chitosan oligosaccharide enzymolysis device suitable for preparing chitosan oligosaccharides, comprising an enzymolysis tank and a reaction barrel arranged in the enzymolysis tank, wherein the enzymolysis tank comprises a tank body, a sealing door arranged on the top surface of the tank body, and a water pipe arranged on the side wall of the tank body near the bottom, wherein a stirring assembly for uniformly mixing reactants is arranged in the reaction barrel, wherein the stirring assembly comprises a rotating shaft arranged in the middle of the reaction barrel, a driving structure for driving the rotating shaft to rotate, and two stirring rod structures staggered up and down;

[0007] The top end of the rotating shaft passes through the top surface of the tank body, and a driven tooth is coaxially fixed to the side wall portion of the rotating shaft located outside the tank body. The driving structure includes a motor fixed to the top surface of the tank body and a driving tooth coaxially fixed to the motor output shaft, and the driving tooth meshes with the driven tooth. The interior of the rotating shaft is hollow and the top end is connected to a wind tube, and a fan blade is arranged inside the wind tube, and a transmission rod is coaxially fixed below the central axis of the fan blade.

[0008] The stirring rod structure comprises a stirring rod and a plurality of stirring blades staggered and regularly distributed on the side wall of the stirring rod, the inner end of the stirring rod extends into the interior of the rotating shaft and a worm wheel is coaxially fixed to the inner end of the stirring rod, a worm is coaxially fixed to the side wall of the transmission rod at a position corresponding to the worm wheel, and the worm is meshed with the worm wheel at a corresponding position;

[0009] The bottom end of the rotating shaft is fixedly connected with a connecting pipe, the bottom end of the connecting pipe is fixedly connected with an air outlet structure, and a plurality of air pipe structures are arranged inside the air outlet structure.

[0010] In the technical solution of the present invention, the reaction barrel includes a barrel body and a discharge pipe arranged in the middle of the bottom surface of the barrel body for controlling the discharge of materials. The discharge pipe passes through the middle of the bottom surface of the tank body and is fixed to the tank body. The sealing door is arranged within the opening range of the top of the barrel body, and a gap is left between the top surface of the barrel body and the top surface of the tank body.

[0011] In the technical solution of the present invention, a clamping groove is provided on the side wall of the rotating shaft near the bottom end, and a clamping ring is fixedly connected to the bottom end of the transmission rod. The clamping ring is arranged in the clamping groove and is rotatable.

[0012] In the technical solution of the present invention, the air outlet structure includes an arc rod, two scraper rods symmetrically arranged on the end faces of the arc rod, and an inner tube arranged in the middle of the outer surface of the arc rod. The outer surface of the arc rod is in contact with the bottom surface of the barrel body, the top end of the scraper rod is L-shaped and the inner end of the upper horizontal section is fixed to the side wall of the rotating shaft, and the vertical section of the scraper rod is in contact with the inner wall of the barrel body on the outside.

[0013] In the technical solution of the present invention, a cavity is provided inside the arc rod, the cavity is connected to the inside of the rotating shaft through the connecting tube, the top end of the inner tube is connected to the inside of the cavity, and the bottom end of the inner tube extends into the discharge tube.

[0014] In the technical solution of the present invention, a secondary air pipe structure is provided on the left side of the top surface of the tank body, and the bottom end of the secondary air pipe structure is connected to the interior of the tank body. The secondary air pipe structure has the same structure as the air pipe structure. The air pipe structure includes a lower tube, a spherical tube and a sealing mechanism for sealing the lower tube. The inner end of the lower tube is connected to the interior of the cavity, and the outer end of the lower tube is connected to the corresponding spherical tube.

[0015] In the technical solution of the present invention, the blocking mechanism is arranged in the spherical tube, and the blocking mechanism includes a fixing rod, a blocking ball slidably mounted on the fixing rod, and a spring mounted on the fixing rod.

[0016] In the technical solution of the present invention, the diameters of the upper and lower ends of the spherical tube are the same as the diameter of the lower tube and are both smaller than the diameter of the middle part of the spherical tube. The diameter of the blocking ball is smaller than the diameter of the middle part of the spherical tube and larger than the diameter of the lower tube.

[0017] In the technical solution of the present invention, mounting frames are fixed to the upper and lower ends of the fixing rod, the mounting frame at the top end is fixed to the inner wall of the spherical tube by the top, and the mounting frame at the bottom end is fixed to the inner wall of the lower tube by the top, and the spring is arranged between the mounting frame at the top end and the sealing ball.

[0018] In the technical solution of the present invention, the side wall of the sealing ball is symmetrically provided with a hinged rod, the top of the hinged rod is provided with a sealing cover, the upper and lower ends of the hinged rod are hinged, the sealing cover is arranged at the top outlet of the spherical tube, and the middle part of the outer side wall of the sealing cover is hinged to the outer side wall of the top of the spherical tube.

[0019] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0020] 1. In the present invention, by setting a stirring assembly, during the reaction, the starting motor drives the rotating shaft to rotate through the meshing driving teeth and driven teeth, and then drives the stirring rod to rotate and stir. At the same time, the air pump is started intermittently to intermittently send the external inert gas into the wind tube. The airflow will blow the fan blades to rotate and drive the transmission rod to rotate, and then drive the worm to rotate. Under the transmission of the worm and worm gear, the stirring rod is driven to rotate and the stirring blade is rotated in the vertical direction, so as to avoid the reaction liquid from vortexing under the action of the stirring rod during stirring, resulting in the inability to quickly convert the inner and outer parts of the reaction liquid, and the reaction liquid on the outer and inner sides is heated unevenly, affecting the quality and rate of enzymatic hydrolysis.

[0021] 2. In the present invention, during the process of introducing inert gas, when the air pressure is greater than the spring force, the sealing ball is lifted up and the lower tube is opened, and the inert gas will be quickly ejected from the gap between the sealing ball and the middle part of the spherical tube. The ejected gas can not only discharge the oxygen in the tank body, but the rapidly ejected airflow will also drive the reaction liquid at the bottom to churn rapidly, thereby causing the upper and lower parts of the reaction liquid to change positions rapidly, so that the reaction liquid is heated more evenly and the quality of enzymatic hydrolysis is improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0023] Figure 2 It is a schematic diagram of the internal structure of the overall structure of the present invention;

[0024] Figure 3 This is a schematic diagram of the interior of the reaction barrel in the present invention;

[0025] Figure 4 It is a cross-sectional view of the transfer shaft of the present invention;

[0026] Figure 5 For the present invention Figure 4 Enlarged view of point A in the middle;

[0027] Figure 6 For the present invention Figure 4 Enlarged view of point B in the middle;

[0028] Figure 7 It is a schematic diagram of the gas outlet structure in the present invention;

[0029] Figure 8 It is a cross-sectional view of the gas outlet structure in the present invention;

[0030] Fig. 9 It is a cross-sectional view of the trachea structure in the present invention;

[0031] Fig.10 It is an exploded view of the trachea structure in the present invention;

[0032] Description of reference numerals:

[0033] 1. Enzyme hydrolysis tank; 11. Tank body; 12. Sealed door; 13. Water pipe; 14. Auxiliary air pipe structure;

[0034] 2. Reaction barrel; 21. Barrel body; 22. Discharge pipe;

[0035] 3. Stirring assembly; 31. Driving structure; 311. Motor; 312. Active gear; 32. Air cylinder; 33. Rotating shaft; 331. Driven gear; 332. Slot; 34. Connecting pipe; 35. Air outlet structure; 351. Arc rod; 352. Scraper rod; 353. Inner tube; 354. Cavity; 36. Air pipe structure; 361. Lower tube; 362. Spherical tube; 363. Blocking mechanism; 3631. Fixed rod; 3632. Mounting frame; 3633. Blocking ball; 3634. Spring; 3635. Sealing cover; 3636. Articulated rod; 37. Fan blade; 38. Transmission rod; 381. Worm; 382. Snap ring; 39. Stirring rod structure; 391. Stirring rod; 392. Stirring blade; 393. Worm gear. DETAILED DESCRIPTION

[0036] The specific implementation modes of the present invention are described in detail below in conjunction with the accompanying drawings, but it should be understood that the protection scope of the present invention is not limited by the specific implementation modes.

[0037] Unless explicitly stated otherwise, throughout the specification, the term “comprise” or variations thereof such as “include” or “comprising” will be understood as including the stated elements or components but not excluding other elements or components.

[0038] Reference Figure 1-Figure 10 As shown, this embodiment provides a technical solution:

[0039] A chitosan oligosaccharide enzymolysis device suitable for preparing chitosan oligosaccharides comprises an enzymolysis tank 1 and a reaction barrel 2 arranged in the enzymolysis tank 1, wherein the enzymolysis tank 1 comprises a tank body 11, a sealing door 12 arranged on the top surface of the tank body 11 and a water pipe 13 arranged on the side wall of the tank body 11 near the bottom, a stirring assembly 3 for uniformly mixing reactants is arranged in the reaction barrel 2, the stirring assembly 3 comprises a rotating shaft 33 arranged in the middle of the reaction barrel 2, a driving structure 31 for driving the rotating shaft 33 to rotate and two stirring rod structures 39 staggered up and down, the tank body 11 and the reaction barrel 2 are heated by a water bath, and water is injected into the tank body 11 through the water pipe 13 before chitosan is enzymolyzed, and it is noted that the water injection liquid level shall not exceed the top of the reaction barrel 2, and the heating means is the prior art of the enzymolysis device, which will not be described in detail here;

[0040] The top end of the rotating shaft 33 passes through the top surface of the tank body 11, and a driven tooth 331 is coaxially fixed to the side wall portion of the rotating shaft 33 located outside the tank body 11. The driving structure 31 includes a motor 311 fixed to the top surface of the tank body 11 and a driving tooth 312 coaxially fixed to the output shaft of the motor 311. The driving tooth 312 is meshed with the driven tooth 331. During enzymolysis, the starting motor 311 drives the rotating shaft 33 to rotate through the meshing driving tooth 312 and the driven tooth 331, thereby driving the stirring rod 391 to rotate and stir;

[0041] The interior of the rotating shaft 33 is hollow and the top end is connected to the wind tube 32. The wind tube 32 is provided with a fan blade 37. A transmission rod 38 is coaxially fixed below the central axis of the fan blade 37. The top end of the wind tube 32 is connected to an external air inlet device, and the external inert gas is intermittently delivered into the wind tube 32 through an air pump.

[0042] The stirring rod structure 39 includes a stirring rod 391 and a plurality of stirring blades 392 staggered and regularly distributed on the side wall of the stirring rod 391. The inner end of the stirring rod 391 extends into the interior of the rotating shaft 33 and a worm gear 393 is coaxially fixed to the inner end of the stirring rod 391. A worm 381 is coaxially fixed to the side wall of the transmission rod 38 at a position corresponding to the worm gear 393. The worm 381 is meshed with the worm gear 393 at a corresponding position. When the air pump delivers inert gas, the airflow will blow the fan blade 37 to rotate and drive the transmission rod 38 to rotate, thereby driving the worm 381 to rotate. Under the transmission of the worm 381 and the worm gear 393, the stirring rod 391 will be driven to rotate and the stirring blades 392 will be driven to rotate in the vertical direction, so as to avoid the reaction liquid from vortexing under the action of the stirring rod 391 during stirring, resulting in the inability to quickly convert the inner and outer parts of the reaction liquid, resulting in uneven heating of the outer and inner reaction liquids, which affects the quality and rate of enzymatic hydrolysis.

[0043] The bottom end of the rotating shaft 33 is fixedly connected to a connecting pipe 34, and the bottom end of the connecting pipe 34 is fixedly connected to an air outlet structure 35. A plurality of air pipe structures 36 are provided inside the air outlet structure 35. By providing the air outlet structure 35 and the air pipe structure 36, not only can inert gas be filled into the tank body 11 during the enzymatic hydrolysis reaction to reduce the internal oxygen, but also non-enzymatic browning can be avoided to affect the production quality of chitosan oligosaccharides.

[0044] Among them, Figure 1-Figure 3 As shown, the reaction barrel 2 includes a barrel body 21 and a discharge pipe 22 arranged in the middle of the bottom surface of the barrel body 21 for controlling the discharge of materials. A control valve is arranged inside the discharge pipe 22, and the bottom end of the inner tube 353 abuts against the valve plate of the control valve to prevent the barrel body 21 from being directly connected to the cavity 354 through the inner tube 353, resulting in a failure to introduce inert gas. The discharge pipe 22 passes through the middle of the bottom surface of the tank body 11 and is fixed to the tank body 11, so that the entire reaction barrel 2 can be fixed to the tank body 11. The sealing door 12 is arranged in the top opening range of the barrel body 21, and a gap is left between the top surface of the barrel body 21 and the top surface of the tank body 11 to prevent the barrel body 21 from being sealed when the inert gas is introduced into the barrel body 21, resulting in a failure to introduce inert gas.

[0045] In addition, if Figure 4-Figure 6 As shown, a groove 332 is provided near the bottom end of the side wall of the rotating shaft 33, and a snap ring 382 is fixedly connected to the bottom end of the transmission rod 38. The snap ring 382 is arranged in the groove 332 and the snap ring 382 is rotatable. By providing the snap ring 382 and the groove 332, the entire transmission rod 38 can be supported without affecting the rotation of the transmission rod 38.

[0046] In addition, if Figure 7-10 As shown, the air outlet structure 35 includes an arc rod 351, two scraper rods 352 symmetrically arranged at the end faces of both ends of the arc rod 351, and an inner tube 353 arranged in the middle of the outer surface of the arc rod 351. The outer surface of the arc rod 351 is in contact with the bottom surface of the barrel body 21. The top of the scraper rod 352 is L-shaped and the inner end of the upper horizontal section is fixed to the side wall of the rotating shaft 33. The vertical section of the scraper rod 352 is in contact with the inner wall of the barrel body 21 on the outside. The arc rod 351 and the scraper rod 352 will rotate synchronously with the rotating shaft 33. During the rotation process, the inner wall of the barrel body 21 can be cleaned to prevent the adhesion of suspended matter.

[0047] Furthermore, a cavity 354 is provided inside the arc rod 351, and the cavity 354 is connected to the inside of the rotating shaft 33 through the connecting pipe 34. The top of the inner tube 353 is connected to the inside of the cavity 354, and the bottom of the inner tube 353 extends into the discharge pipe 22. The air pump intermittently delivers the external inert gas into the air cylinder 32, and delivers it into the cavity 354 through the rotating shaft 33 and the connecting pipe 34. During the closing process of the sealing cover 3635, a small amount of reaction liquid will inevitably enter the spherical tube 362. During the falling and sealing process of the blocking ball 3633, this small amount of reaction liquid will enter the cavity 354 and flow into the inner tube 353 under the action of gravity, and can be released when discharging.

[0048] In addition, a secondary air pipe structure 14 is provided on the left side of the top surface of the tank body 11, and the bottom end of the secondary air pipe structure 14 is connected to the inside of the tank body 11. The secondary air pipe structure 14 has the same structure as the air pipe structure 36. By providing the secondary air pipe structure 14, it is possible to prevent external air from entering the inside of the tank body 11 and affecting the reaction. At the same time, when the air pressure inside the tank body 11 is too high, excess gas can be discharged to prevent the subsequent air pressure inside the tank body 11 from being too high and being unable to continuously introduce inert gas.

[0049] Specifically, the air pipe structure 36 includes a lower tube 361, a spherical tube 362, and a blocking mechanism 363 for blocking the lower tube 361. The inner end of the lower tube 361 is connected to the inside of the cavity 354, and the outer end of the lower tube 361 is connected to the corresponding spherical tube 362. By setting the blocking mechanism 363, it can be prevented that during enzymatic hydrolysis, the reaction liquid enters the cavity 354 through the lower tube 361 and the spherical tube 362, resulting in the inability to pass inert gas to discharge oxygen;

[0050] Furthermore, the blocking mechanism 363 is arranged in the spherical tube 362, and the blocking mechanism 363 includes a fixed rod 3631, a blocking ball 3633 which is sleeved on the fixed rod 3631 and can slide, and a spring 3634 which is sleeved on the fixed rod 3631. The diameters of the upper and lower ends of the spherical tube 362 are the same as the diameter of the lower tube 361 and are both smaller than the diameter of the middle part of the spherical tube 362. The diameter of the blocking ball 3633 is smaller than the diameter of the middle part of the spherical tube 362 and larger than the diameter of the lower tube 361. When the inert gas is introduced, when the gas pressure is greater than the elastic force of the spring 3634, the blocking ball 3633 is lifted up and the lower tube 361 is opened. At this time, the inert gas can be quickly ejected from the gap between the blocking ball 3633 and the middle part of the spherical tube 362. The ejected gas can not only discharge the oxygen in the tank body 11, but also drive the reaction liquid at the bottom to churn rapidly, thereby causing the upper and lower parts of the reaction liquid to change positions rapidly, so that the reaction liquid is heated more evenly and the quality of enzymatic hydrolysis is improved.

[0051] In addition, mounting brackets 3632 are fixed to the upper and lower ends of the fixing rod 3631. The top mounting bracket 3632 is fixed to the inner wall of the spherical tube 362 by the top, and the bottom mounting bracket 3632 is fixed to the inner wall of the lower tube 361 by the top. The spring 3634 is arranged between the top mounting bracket 3632 and the blocking ball 3633. The fixing rod 3631 is fixed by arranging the mounting bracket 3632 to prevent the fixing rod 3631 from shaking. The spring 3634 is arranged so that when the inert gas is not introduced, the blocking ball 3633 completely blocks the lower tube 361 to prevent the reaction liquid from entering.

[0052] Furthermore, the side wall of the sealing ball 3633 is symmetrically provided with a hinged rod 3636, and a sealing cover 3635 is provided at the top of the hinged rod 3636. The upper and lower ends of the hinged rod 3636 are hinged, and the sealing cover 3635 is covered at the top outlet of the spherical tube 362. The middle part of the outer wall of the sealing cover 3635 is hinged to the outer wall of the top of the spherical tube 362. When chitosan is hydrolyzed into chitosan oligosaccharides, the reaction liquid is weakly acidic. When the sealing ball 3633 blocks the lower tube 361, the sealing cover 3635 will be pulled to close quickly through the hinged rod 3636 to prevent the spring 3634 from being soaked in the reaction liquid and affecting the service life of the spring 3634.

[0053] The working principle of the chitosan oligosaccharide enzymatic hydrolysis device suitable for preparing chitosan oligosaccharides of the present invention is specifically as follows:

[0054] During preparation, water is first injected into the tank body 11 through the water pipe 13, and then the heating device is turned on to heat the water in the tank body 11. After heating to a suitable temperature, the sealing door 12 is opened to add a chitosan solution, an enzyme solution and a buffer solution in a suitable ratio into the reaction barrel 2;

[0055] Then, the sealing door 12 is closed, and the motor 311 is started to drive the rotating shaft 33 to rotate through the meshing driving teeth 312 and the driven teeth 331, thereby driving the stirring rod 391 to rotate and stir, and at the same time, the air pump is intermittently started to intermittently deliver the external inert gas into the air cylinder 32. When the air pump delivers the inert gas, the airflow will blow the fan blades 37 to rotate and drive the transmission rod 38 to rotate, thereby driving the worm 381 to rotate. Under the transmission of the worm 381 and the worm wheel 393, the stirring rod 391 will be driven to rotate and drive the stirring blade 392 to rotate in the vertical direction, so as to avoid the reaction liquid from vortexing under the action of the stirring rod 391 during stirring, resulting in the inability to quickly convert the inner and outer parts of the reaction liquid, resulting in uneven heating of the reaction liquid on the outer and inner sides, affecting the quality and rate of enzymatic hydrolysis;

[0056] During the process of introducing inert gas, when the air pressure is greater than the elastic force of the spring 3634, the sealing ball 3633 is lifted up and the lower tube 361 is opened. At this time, the inert gas can be quickly ejected from the gap between the sealing ball 3633 and the middle part of the spherical tube 362. The ejected gas can not only discharge the oxygen in the tank body 11, but the rapidly ejected airflow can also drive the reaction liquid at the bottom to churn rapidly, thereby causing the upper and lower parts of the reaction liquid to quickly change positions, so that the reaction liquid is heated more evenly and the quality of enzymatic hydrolysis is improved. When the inert gas is not introduced, the sealing ball 3633 completely blocks the lower tube 361 to prevent the reaction liquid from entering. At the same time, the sealing ball 3633 pulls the sealing cover 3635 to close quickly through the hinged rod 3636 to prevent the spring 3634 from being soaked in the reaction liquid and affecting the service life of the spring 3634.

[0057] The foregoing description of specific exemplary embodiments of the present invention is for the purpose of illustration and demonstration. These descriptions are not intended to limit the present invention to the precise form disclosed, and it is clear that many changes and variations can be made based on the above teachings. The purpose of selecting and describing exemplary embodiments is to explain the specific principles of the present invention and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the present invention and various different selections and changes. The scope of the present invention is intended to be limited by the specification and its equivalents.

Claims

1. A chitosan oligosaccharide enzymolysis device suitable for preparing chitosan oligosaccharides, comprising an enzymolysis tank (1) and a reaction barrel (2) arranged in the enzymolysis tank (1), wherein the enzymolysis tank (1) comprises a tank body (11), a sealing door (12) arranged on the top surface of the tank body (11), and a water pipe (13) arranged on the side wall of the tank body (11) near the bottom, characterized in that: The reaction barrel (2) comprises a barrel body (21) and a discharge pipe (22) arranged in the middle of the bottom surface of the barrel body (21) for controlling discharge, the discharge pipe (22) passing through the middle of the bottom surface of the tank body (11) and being fixed to the tank body (11), the sealing door (12) being arranged within the top opening range of the barrel body (21), a gap being left between the top surface of the barrel body (21) and the top surface of the tank body (11), a stirring assembly (3) for uniformly mixing the reactants being arranged in the reaction barrel (2), the stirring assembly (3) comprising a rotating shaft (33) arranged in the middle of the reaction barrel (2), a driving structure (31) for driving the rotating shaft (33) to rotate, and two stirring rod structures (39) staggeredly arranged up and down; The top end of the rotating shaft (33) passes through the top surface of the tank body (11); a driven tooth (331) is coaxially fixed to a side wall portion of the rotating shaft (33) located outside the tank body (11); the driving structure (31) comprises a motor (311) fixed to the top surface of the tank body (11) and a driving tooth (312) coaxially fixed to an output shaft of the motor (311); the driving tooth (312) meshes with the driven tooth (331); the interior of the rotating shaft (33) is hollow and the top end is connected to a wind tube (32); a fan blade (37) is provided inside the wind tube (32); a transmission rod (38) is coaxially fixed below the central axis of the fan blade (37); The stirring rod structure (39) comprises a stirring rod (391) and a plurality of stirring blades (392) staggered and regularly distributed on the side wall of the stirring rod (391); the inner end of the stirring rod (391) extends into the interior of the rotating shaft (33) and a worm wheel (393) is coaxially fixed to the inner end of the stirring rod (391); a worm (381) is coaxially fixed at a position of the side wall of the transmission rod (38) corresponding to the position of the worm wheel (393); the worm (381) is meshed with the worm wheel (393) at a corresponding position; a groove (332) is provided near the bottom end of the side wall of the rotating shaft (33); a snap ring (382) is fixedly connected to the bottom end of the transmission rod (38); the snap ring (382) is arranged in the groove (332) and is rotatable; The bottom end of the rotating shaft (33) is fixedly connected to a connecting tube (34), and the bottom end of the connecting tube (34) is fixedly connected to an air outlet structure (35), the air outlet structure (35) comprising an arc rod (351), two scraping rods (352) symmetrically arranged at the end surfaces of both ends of the arc rod (351), and an inner tube (353) arranged in the middle of the outer surface of the arc rod (351), the arc rod (351) is provided with a cavity (354) inside, the cavity (354) is communicated with the inside of the rotating shaft (33) through the connecting tube (34), the top end of the inner tube (353) is communicated with the inside of the cavity (354), and the bottom end of the inner tube (353) extends into the discharge tube (22), and a plurality of air pipe structures (36) are provided inside the air outlet structure (35); The trachea structure (36) comprises a lower tube (361), a spherical tube (362), and a blocking mechanism (363) for blocking the lower tube (361); the inner end of the lower tube (361) is in communication with the interior of the cavity (354), and the outer end of the lower tube (361) is in communication with the corresponding spherical tube (362); the blocking mechanism (363) comprises a fixing rod (3631), a blocking ball (3633) sleeved on the fixing rod (3631) and slidable, and a spring (3634) sleeved on the fixing rod (3631); the diameters of the upper and lower ends of the spherical tube (362) are the same as the diameter of the lower tube (361) and are both smaller than the diameter of the middle portion of the spherical tube (362); the diameter of the blocking ball (3633) is smaller than the diameter of the middle portion of the spherical tube (362) and larger than the diameter of the lower tube (361).

2. The chitosan oligosaccharide enzymolysis device suitable for preparing chitosan oligosaccharides according to claim 1, characterized in that: The outer surface of the arc rod (351) is in contact with the bottom surface of the barrel body (21); the top end of the scraper rod (352) is L-shaped and the inner end of the upper horizontal section is fixed to the side wall of the rotating shaft (33); the vertical section of the scraper rod (352) is in contact with the inner wall of the barrel body (21) on the outer side.

3. The chitosan oligosaccharide enzymolysis device suitable for chitosan oligosaccharide preparation as claimed in claim 2, characterized in that: A secondary air pipe structure (14) is provided on the left side of the top surface of the tank body (11); the bottom end of the secondary air pipe structure (14) is in communication with the interior of the tank body (11); and the secondary air pipe structure (14) has the same structure as the air pipe structure (36).

4. The chitosan oligosaccharide enzymolysis device suitable for preparing chitosan oligosaccharides as claimed in claim 3, characterized in that: The upper and lower ends of the fixing rod (3631) are both fixed with mounting frames (3632); the mounting frame (3632) at the top end is fixed to the inner wall of the spherical tube (362) by the top, and the mounting frame (3632) at the bottom end is fixed to the inner wall of the lower tube (361) by the top; the spring (3634) is arranged between the mounting frame (3632) at the top end and the blocking ball (3633).

5. The chitosan oligosaccharide enzymolysis device suitable for preparing chitosan oligosaccharides as claimed in claim 4, characterized in that: The side wall of the blocking ball (3633) is symmetrically provided with a hinged rod (3636), and the top of the hinged rod (3636) is provided with a sealing cover (3635). The upper and lower ends of the hinged rod (3636) are hinged, and the sealing cover (3635) is covered at the top outlet of the spherical tube (362). The middle part of the outer wall of the sealing cover (3635) is hinged to the outer wall of the top of the spherical tube (362).

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