Device and method for extracting leech salivary gland secretions

Abstract

The present invention relates to the technical field of hirudin extraction, and proposes a device and method for extracting leech salivary gland secretions, including a recovery box, a skin fluid collection box and a saliva collection box, wherein the recovery box is provided with a skin fluid recovery chamber and a saliva recovery chamber, the bottom of the skin fluid collection box is connected to the skin fluid recovery chamber via a connecting pipe, and the saliva collection box is connected to the saliva recovery chamber via a discharge pipe. The present invention can extract skin gland fluid while performing saliva-assisted extraction by setting a skin fluid collection box and a saliva collection box, firstly utilizing the characteristics of leeches that like quietness and avoid light, so that the skin gland secretions are scraped off when the leeches drill into the saliva collection box, and then by feeding an inducer and automatically pressing the leeches to vomit the saliva secretions, the integrated collection method is convenient to use.

Description

Technical Field

[0001] The invention relates to the technical field of hirudin extraction, in particular to a device and a method for extracting leech salivary gland secretions. Background Art

[0002] Hirudin is named after the leech from which it was first isolated. Traditionally, the acquisition of hirudin is a one-time plunder of leeches, which causes a huge waste of wild leech resources and is not conducive to the development of leech breeding. In order to avoid harming leeches and to extract natural hirudin an unlimited number of times during their growth period so that their population can multiply and expand, some methods for multiple extraction of leeches have been proposed through research, such as feeding leeches with inducers and then pressing them to make them spit out crude saliva secretions or through electrical stimulation.

[0003] When extracting saliva secretions by pressing, the manual pressing extraction method is inefficient. Meanwhile, the skin fluid of leeches also has very high medicinal value. However, the prior art cannot extract the skin gland secretions separately while extracting the leech saliva secretions. Summary of the invention

[0004] In view of the deficiencies of the prior art, the present invention provides a device and method for extracting leech salivary gland secretions, which solves the problem that the manual pressing extraction method is inefficient when extracting salivary secretions, and the prior art cannot extract skin gland secretions separately while extracting leech salivary secretions.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions: a device for extracting leech salivary gland secretions, comprising a recovery box, a skin fluid collection box and a saliva collection box, wherein a skin fluid recovery chamber and a saliva recovery chamber are provided inside the recovery box, the bottom of the skin fluid collection box is connected to the skin fluid recovery chamber through a connecting pipe, and the saliva collection box is connected to the saliva recovery chamber through a discharge pipe;

[0006] The skin fluid collection box is provided with an illumination lamp and a sound generator, and a plurality of through holes are provided on the side wall where the skin fluid collection box and the saliva collection box are in contact, so as to allow leeches to pass through;

[0007] An induction liquid nozzle is installed on the side wall of the saliva collection box for spraying the induction liquid. An electric slide rail is installed inside the saliva collection box. A retractable pressing mechanism is detachably connected to the lower surface of the electric slide rail. The retractable pressing mechanism includes a sealing cover. A cylinder is arranged inside the sealing cover. The cylinder is slidably connected to a slide seat on the inner wall of the sealing cover. The telescopic end of the cylinder passes through the sealing cover and is connected to a pressing plate mechanism, which is used to drive the pressing plate mechanism to descend to contact with the leech. A reciprocating pressing assembly is installed on the side wall of the sealing cover, which is used to drive the pressing plate mechanism to reciprocate and press the leech.

[0008] The pressing plate mechanism includes a connecting vertical rod, a hollow main pressing plate is fixed at the bottom of the connecting vertical rod, and side scrapers are slidably connected to the four sides of the hollow main pressing plate. The side scrapers and the bottom of the hollow main pressing plate are located on the same plane, and a scraper telescopic assembly is arranged inside the hollow main pressing plate. An energizing assembly is installed on the surface of the cylinder. When the telescopic end of the cylinder is retracted, the energizing assembly is energized to make the scraper telescopic assembly drive the side scrapers to retract. When the telescopic end of the cylinder is extended, the energizing assembly is de-energized to make the scraper telescopic assembly drive the side scrapers to extend.

[0009] A spray flushing mechanism is provided on the top of the saliva collection box, and the spray flushing mechanism extends to the skin fluid collection box and the saliva collection box respectively, and is used to flush the collected skin fluid and saliva;

[0010] A refrigeration unit is commonly provided at the back of the skin fluid collection box and the saliva collection box. The refrigeration unit is connected with a conducting pipe, and the conducting pipe is communicated with the skin fluid collection box and the saliva collection box respectively.

[0011] Preferably, the spray flushing mechanism includes a spray main pipe arranged on the top of the saliva collection box, a saliva flushing head and a first skin liquid flushing head arranged inside the saliva collection box, and a second skin liquid flushing head arranged inside the skin liquid collection box, the saliva flushing head is installed on the top of the saliva collection box, the second skin liquid flushing head is installed on the upper side wall of the through hole, and the first skin liquid flushing head is movably arranged on one side of the through hole through a movable component.

[0012] Preferably, the movable component includes a telescopic cylinder fixed on the inner wall of the saliva collection box, a blocking plate is fixed to the telescopic end of the telescopic cylinder, the first skin liquid flushing head is installed on the outer surface of the blocking plate, and a plurality of nozzles matching the through holes are arranged on the first skin liquid flushing head. The nozzles extend into the grooves on the surface of the blocking plate and are used to flush the skin fluid inside the through holes.

[0013] Preferably, the reciprocating pressing assembly includes a motor fixed on the outer wall of the sealing cover, the driving end of the motor extends to the inner wall of the sealing cover and is fixedly connected to a turntable, the surface of the turntable is rotatably connected to a rotating rod, and the surface of the cylinder is fixed with a connecting block, which is rotatably connected to the rotating rod.

[0014] Preferably, the scraper telescopic assembly is divided into four groups, which are respectively arranged on one side of the four side scrapers. The driving telescopic assembly includes a partition fixed inside the hollow main pressure plate, and the partition is connected to the side scraper through a spring. A first electromagnet block is fixed on the side wall of the partition, and a second electromagnet block is fixed on the side wall of the side scraper.

[0015] Preferably, the power-carrying component includes a first fixed plate fixed to the bottom end of the cylinder shell and a second fixed plate fixed to the bottom of the telescopic end of the cylinder, the lower surface of the first fixed plate is fixed with a conductive groove, and the upper surface of the second fixed plate is fixed with a conductive block matching the conductive groove.

[0016] Preferably, a feed cover is rotatably connected to the top of the skin fluid collection box, an observation window is provided on the surface of the saliva collection box, and a discharge door is rotatably connected thereto, and a filter is provided at one end of the discharge pipe and the connecting pipe.

[0017] A method for extracting leech salivary gland secretions, comprising the following specific operating steps:

[0018] S1, first put the live leech into the skin fluid collection box, start the refrigeration unit to reduce the temperature inside the skin fluid collection box and the saliva collection box, turn on the lighting and sound generator, and make the leech pass through the hole into the dim saliva collection box by taking advantage of the leech's quiet and light-avoiding characteristics. During the process of passing through the hole, the secretions of the leech's skin glands will be scraped off;

[0019] S2, after the leeches have completely entered the saliva collection box, the induction liquid is sprayed out through the induction liquid nozzle to feed the leeches. While feeding the induction liquid, the telescopic rod is extended to push the blocking plate to move and block the through hole, and the skin gland secretions at the through hole are flushed into the skin liquid recovery chamber through the first skin liquid flushing head and the second skin liquid flushing head;

[0020] S3, after the induction liquid is fed, the cylinder is started to move the pressing plate mechanism downward to contact the leech at the bottom of the saliva collection box, and then the reciprocating pressing assembly is started to press multiple times to make the leech spit out the saliva secretions, and then the saliva secretions can be flushed into the saliva recovery chamber through the saliva flushing head;

[0021] S4. The body surface secretions and saliva secretions are discharged separately and filtered and collected, and the glycoproteins and polypeptide substances in the body surface secretions are processed and purified, and the saliva secretions are separated and purified according to conventional biochemical separation methods to obtain raw material products.

[0022] The present invention provides a device and method for extracting leech salivary gland secretions.

[0023] Beneficial effects:

[0024] 1. The present invention can extract skin gland fluid simultaneously with saliva-assisted extraction by setting a skin fluid collection box and a saliva collection box. The leeches are firstly made use of their quiet and light-avoiding characteristics to scrape off the skin gland secretions when drilling into the saliva collection box. Then, the leeches are fed with an inducer and automatically pressed to cause them to vomit the saliva secretions. The integrated collection method is convenient to use. Meanwhile, the refrigeration unit can be set to keep the inside of the skin fluid collection box and the saliva collection box at a low temperature at all times, and the low temperature can greatly increase the activity of the leeches.

[0025] 2. The present invention is provided with a pressing plate mechanism, a cylinder, and a reciprocating pressing assembly. The cylinder moves downward to drive the pressing plate mechanism to contact the leech, and the reciprocating pressing assembly reciprocates to continuously and lightly press the leech, so that the leech spits out saliva secretions. Compared with manual pressing, the speed is faster and the work efficiency is greatly improved.

[0026] 3. The present invention arranges side scrapers, a scraper telescopic assembly and an energizing assembly to prevent leeches crawling on the inner wall of the saliva collection box from being unable to be pressed. Therefore, when the reciprocating pressing mechanism moves downward, the energizing assembly is powered off, and the side scrapers are extended to contact the inner wall of the saliva collection box. The leeches crawling on the inner wall of the saliva collection box can be scraped off and pressed to make them spit out saliva secretions, thereby preventing the leeches from crawling on the inner wall of the saliva collection box and being unable to be pressed. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 A stereoscopic diagram of a device for extracting leech salivary gland secretions according to the present invention;

[0028] Figure 2 It is a main cross-sectional view of a device for extracting leech salivary gland secretions according to the present invention;

[0029] Figure 3 A top view of a device for extracting leech salivary gland secretions according to the present invention;

[0030] Figure 4 It is a stereoscopic diagram of a reciprocating pressing assembly in a device for extracting leech salivary gland secretions according to the present invention;

[0031] Figure 5It is a front cross-sectional view of a retractable pressing mechanism and a pressing plate mechanism in a device for extracting leech salivary gland secretions according to the present invention;

[0032] Figure 6 It is a side cross-sectional view of a retractable pressing mechanism and a pressing plate mechanism in a device for extracting leech salivary gland secretions of the present invention;

[0033] Figure 7 This is an enlarged view of point A in a device for extracting leech salivary gland secretions according to the present invention.

[0034] Among them, 1. recovery box; 11. skin fluid recovery chamber; 12. saliva recovery chamber; 13. discharge pipe; 2. skin fluid collection box; 21. feed cover; 22. sound generator; 23. through hole; 24. lighting lamp; 3. saliva collection box; 31. electric slide rail; 32. retractable pressing mechanism; 321. cylinder; 322. reciprocating pressing assembly; 3221. motor; 3222. turntable; 3223. rotating rod; 3224. connecting block; 323. sealing cover; 324. slide seat; 325. first fixed plate; 326. second fixed plate; 327. Conductive block; 328. Conductive slot; 33. Pressing plate mechanism; 331. Connecting vertical rod; 332. Hollow main pressure plate; 333. Side scraper; 334. Scraper telescopic assembly; 3341. Spring; 3342. First electromagnet block; 3343. Partition; 3344. Second electromagnet block; 34. Inducing liquid nozzle; 4. Spray flushing mechanism; 41. Spray main pipe; 42. Saliva flushing head; 43. First skin liquid flushing head; 44. Second skin liquid flushing head; 5. Telescopic cylinder; 6. Blocking plate; 7. Refrigeration unit; 8. Conductive pipe. DETAILED DESCRIPTION

[0035] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0036] Embodiment 1:

[0037] like Figure 1-Figure 6As shown, an embodiment of the present invention provides a device for extracting leech salivary gland secretions, comprising a recovery box 1, a skin fluid collection box 2 and a saliva collection box 3. The recovery box 1 is provided with a skin fluid recovery chamber 11 and a saliva recovery chamber 12. The bottom of the skin fluid collection box 2 is connected to the skin fluid recovery chamber 11 through a connecting pipe, and the saliva collection box 3 is connected to the saliva recovery chamber 12 through a discharge pipe 13. Valves are installed on the surfaces of the connecting pipe and the discharge pipe 13, which can conveniently direct the collected secretions to the respective The skin fluid collection chamber 11 and the saliva collection chamber 12 are entered, and a lighting lamp 24 and a sound generator 22 are installed inside the skin fluid collection box 2. Taking advantage of the characteristics of Philippine leeches that they like quietness and avoid light, under light and noise, Philippine leeches will naturally want to hide in relatively quiet and dark places. The inner wall of the saliva collection box 3 can be black, which is more shading and more popular with Philippine leeches. A number of through holes 23 are opened on the side walls where the skin fluid collection box 2 and the saliva collection box 3 are in contact, for the Philippine leeches to pass through;

[0038] An induction liquid nozzle 34 is installed on the side wall of the saliva collection box 3 for spraying the induction liquid. An electric slide rail 31 is installed inside the saliva collection box 3. The lower surface of the electric slide rail 31 is detachably connected to a retractable pressing mechanism 32. The retractable pressing mechanism 32 includes a sealing cover 323. A cylinder 321 is arranged inside the sealing cover 323. The cylinder 321 is slidably connected to a slide seat 324 on the inner wall of the sealing cover 323. The retractable end of the cylinder 321 passes through the sealing cover 323 and is connected to a pressing plate mechanism 33 for driving the pressing plate mechanism 33 to move downward. The sealing cover 323 is lowered to contact with the leech. A reciprocating pressing assembly 322 is installed on the side wall of the sealing cover 323, which is used to drive the pressing plate mechanism 33 to reciprocate and press the leech. The reciprocating pressing assembly 322 includes a motor 3221 fixed on the outer wall of the sealing cover 323. The driving end of the motor 3221 extends to the inner wall of the sealing cover 323 and is fixedly connected to a turntable 3222. The surface of the turntable 3222 is rotatably connected to a rotating rod 3223. A connecting block 3224 is fixed to the surface of the cylinder 321. The connecting block 3224 is rotatably connected to the rotating rod 3223.

[0039] like Figure 2 and Figure 3As shown, in specific use, first, the cylinder 321 drives the pressing plate mechanism 33 to move downward so that the pressing plate mechanism 33 descends to contact the leech on the inner bottom wall of the saliva collection box 3 or is at a small distance from the leech, and then the reciprocating pressing component 322 is started to rotate the motor 3221, driving the rotating disk 3222 to rotate, so that the rotating rod 3223 drives the cylinder 321 to move up and down slightly, and then drives the pressing plate mechanism 33 to move up and down slightly to press the leech, wherein the closest distance between the pressing plate mechanism 33 and the inner bottom wall of the saliva collection box 3 is 1-1 .5CM, which can ensure that the leech can be pressed well and can spit out liquid when pressed. Since the leech itself is soft and has good deformation ability, a small reciprocating pressure will not cause the death of the leech. The leech can spit out crude saliva secretions after being squeezed. The number of reciprocating pressures is 6-8 times. After reaching the preset number of times, the cylinder 321 drives the pressing plate mechanism 33 to move up, and then moves the position driven by the electric slide rail 31, and the operation is repeated again until all positions in the saliva collection box 3 are pressed.

[0040] In order to prove the compression resistance of the leech, a compression test was conducted on the leech. A leech was taken and placed under a wheel. After the wheel repeatedly rolled over both sides, the leech still survived, which can prove that the survivability of the leech is not affected at all by the pressure of the pressing plate mechanism 33.

[0041] A spray flushing mechanism 4 is provided on the top of the saliva collection box 3. The spray flushing mechanism 4 extends to the skin fluid collection box 2 and the saliva collection box 3 respectively, and is used for flushing the collected skin fluid and saliva.

[0042] The spray flushing mechanism 4 includes a spray main pipe 41 arranged on the top of the saliva collection box 3, a saliva flushing head 42 and a first skin liquid flushing head 43 arranged inside the saliva collection box 3, and a second skin liquid flushing head 44 arranged inside the skin liquid collection box 2. The saliva flushing head 42 is installed on the top of the saliva collection box 3, the second skin liquid flushing head 44 is installed on the upper side wall of the through hole 23, and the first skin liquid flushing head 43 is movably arranged on one side of the through hole 23 through a movable component, wherein electromagnetic valves are arranged between the spray main pipe 41 and the saliva flushing head 42, the first skin liquid flushing head 43 and the second skin liquid flushing head 44, the saliva flushing head 42 is used to spray the saliva secretions into the saliva recovery chamber 12 after the Philippine cow leech spits out the crude saliva secretions, the first skin liquid flushing head 43 is used to flush the skin gland secretions inside the through hole 23, and the second skin liquid flushing head 44 is used to flush the skin gland secretions on the surface of one side of the through hole 23.

[0043] The mobile assembly includes a telescopic cylinder 5 fixed on the inner wall of the saliva collection box 3, a blocking plate 6 is fixed to the telescopic end of the telescopic cylinder 5, a first skin liquid flushing head 43 is installed on the outer surface of the blocking plate 6, and a plurality of nozzles matching the through hole 23 are arranged on the first skin liquid flushing head 43. The nozzles extend into the grooves on the surface of the blocking plate 6 and are used to flush the skin liquid inside the through hole 23. When the Philippine leech needs to drill a hole, the telescopic cylinder 5 is in a retracted state (such as Figure 2 As shown in the figure, in order to avoid obstruction, after the leech finishes drilling, the telescopic cylinder 5 extends to drive the blocking plate 6 to move, so that the first skin liquid flushing head 43 can be moved to the through hole 23. At the same time, the blocking plate 6 can push down the leech attached to the side wall surface with the through hole 23 that has not been completely moved to the bottom wall of the saliva collection box 3.

[0044] The top of the skin fluid collection box 2 is rotatably connected with a feed cover 21 for placing the leeches in. The surface of the saliva collection box 3 is provided with an observation window and is rotatably connected with a discharge door for taking out the leeches.

[0045] Embodiment 2:

[0046] In order to solve the problem that some leeches crawl on the side wall of the saliva collection box 3 and cannot be pressed when pressed, the device is further designed based on Example 1:

[0047] like Figure 4-Figure 7As shown, the pressing plate mechanism 33 includes a connecting vertical rod 331, a hollow main pressing plate 332 is fixed at the bottom of the connecting vertical rod 331, and side scrapers 333 are slidably connected to the four sides of the hollow main pressing plate 332, which can scrape off leeches attached to the inner wall of the saliva collection box 3, and the side scrapers 333 and the bottom of the hollow main pressing plate 332 are located on the same plane, so that the side scrapers 333 can also play a role when pressed, and a scraper telescopic component 334 is arranged inside the hollow main pressing plate 332, and an energizing component is installed on the surface of the cylinder 321. When the telescopic end of the cylinder 321 is retracted, the energizing component is energized, so that the scraper telescopic component 334 drives the side scrapers 333 to retract, and when the telescopic end of the cylinder 321 is extended, the energizing component is de-energized, so that the scraper telescopic component 334 drives The movable side scraper 333 extends out, and the scraper telescopic assembly 334 is divided into four groups, which are respectively arranged on one side of the four side scrapers 333. The driving telescopic assembly includes a partition 3343 fixed inside the hollow main pressure plate 332, and the partition 3343 is connected to the side scraper 333 by a spring 3341. A first electromagnet block 3342 is fixed on the side wall of the partition 3343, and a second electromagnet block 3344 is fixed on the side wall of the side scraper 333. The power-on assembly includes a first fixed plate 325 fixed at the bottom end of the shell of the cylinder 321 and a second fixed plate 326 fixed at the bottom of the telescopic end of the cylinder 321. A conductive groove 328 is fixed on the lower surface of the first fixed plate 325, and a conductive block 327 matching the conductive groove 328 is fixed on the upper surface of the second fixed plate 326.

[0048] When in use, the telescopic end of the cylinder 321 is first extended. When the telescopic end of the cylinder 321 is extended a certain distance, the conductive slot 328 can be separated from the conductive block 327, so that the first electromagnet block 3342 and the second electromagnet block 3344 are separated, and the side scraper 333 can be extended to the inner wall of the saliva collection box 3. Then, the pressing plate mechanism 33 continues to move downward, and the leeches on the inner wall of the saliva collection box 3 can be scraped off, which is convenient for pressing to extract saliva. After the pressing is completed, the cylinder 321 retracts. Since the leeches on the inner wall of the saliva collection box 3 have been scraped off, the leeches will not be affected when the side scraper 333 contacts the saliva collection box 3 and moves upward. When it moves to the highest position, the conductive slot 328 and the conductive block 327 are energized again to retract the side scraper 333. At this time, the electric slide rail 31 can be used to move the position, and the operation can be repeated to press the leeches at other positions.

[0049] Embodiment 3:

[0050] Since the activity of Philippine leeches can be increased at low temperatures of -10-0℃, the device is further designed:

[0051] A refrigeration unit 7 is commonly provided on the back of the skin fluid collection box 2 and the saliva collection box 3. The refrigeration unit 7 is connected to a conducting pipe 8, which is respectively connected to the skin fluid collection box 2 and the saliva collection box 3. The refrigeration unit 7 is a commonly used refrigeration unit consisting of four major components: a compressor, a condenser, an evaporator, and an expansion valve. A fan is installed at the evaporator, which can quickly discharge cold air into the skin fluid collection box 2 and the saliva collection box 3 to reduce their internal temperature and increase the activity of the leech. The skin fluid collection box 2 and the saliva collection box 3 are also provided with a temperature sensor which is not shown in the figure. You can try to observe the internal temperature of the skin fluid collection box 2 and the saliva collection box 3.

[0052] Embodiment 4:

[0053] A method for extracting leech salivary gland secretions, comprising the following specific operating steps:

[0054] S1, first put the live leech into the skin fluid collection box 2, and start the refrigeration unit 7 to reduce the temperature inside the skin fluid collection box 2 and the saliva collection box 3. The leech can increase its activity under low temperature. Turn on the lighting 24 and the sound generator 22, and make the leech pass through the hole 23 into the dim saliva collection box 3 by taking advantage of the leech's quiet and light-avoiding characteristics. During the process of passing through the hole 23, the skin gland secretions of the leech will be scraped off;

[0055] S2, after the leech completely enters the saliva collecting box 3, the inducing liquid is sprayed from the inducing liquid nozzle 34 to feed the leech. The inducing liquid is selected as follows: it must have strong inducing property to the living body and can significantly stimulate its feeding to secrete the salivary glands to the maximum extent; it must be non-toxic, harmless, non-irritating, and not affect the separation and purification of natural hirudin to improve its extraction rate. At the same time, substances with antibacterial and anti-inflammatory properties and body constitution enhancement can be added to improve the survival rate of the living body. While feeding the inducing liquid, the telescopic rod 5 is extended to push the blocking plate 6 to move and block the through hole 23, and the skin gland secretions at the through hole 23 are flushed into the skin liquid recovery chamber 11 through the first skin liquid flushing head 43 and the second skin liquid flushing head 44. The extraction is performed 1 to 2 times a month, and about 3-5 ml of skin gland secretions can be extracted from each living leech each time. Each leech can be repeatedly extracted 3 to 5 times.

[0056] S3, after the induction liquid is fed, the cylinder 321 is started to move the pressing plate mechanism 33 downward to contact the leech at the bottom of the saliva collection box 3, and then the reciprocating pressing component 322 is started to press multiple times to make the leech spit out the saliva secretions, and then the saliva secretions can be spit into the saliva recovery chamber 12 through the saliva flushing head 42, and the extraction is performed 1 to 2 times a month, and each living leech can extract 5-10 ml of saliva secretions each time, and the hirudin content in the extracted saliva is 20 to 120 AT-(U international units) / ml. Each leech can be repeatedly extracted 3 to 5 times.

[0057] S4. The body surface secretions and saliva secretions are discharged separately and filtered and collected, and the glycoproteins and polypeptide substances in the body surface secretions are processed and purified, and the saliva secretions are separated and purified according to conventional biochemical separation methods to obtain raw material products.

[0058] After multiple extractions, the saliva secretion of a living body decreases and the content of effective substances also gradually decreases. Therefore, after a certain number of extractions, the living body will be killed, homogenized, and then extracted for the last time, so that its important medicinal value can be brought into play.

[0059] This technology can achieve continuous extraction several times, which not only saves costs but also increases the efficacy of salivary gland secretions, allowing the living Philippine leeches to be reused, solving the current pain points of low efficiency, complex process and high cost of one-time use of salivary gland secretions extraction. It helps to save and protect my country's wild leech resources and help to significantly reduce the cost of Philippine leech raw materials.

[0060] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A device for extracting leech salivary gland secretions, characterized in that: The invention comprises a recovery box (1), a skin fluid collection box (2) and a saliva collection box (3); the recovery box (1) is provided with a skin fluid recovery chamber (11) and a saliva recovery chamber (12); the bottom of the skin fluid collection box (2) is connected to the skin fluid recovery chamber (11) via a connecting pipe; the saliva collection box (3) is connected to the saliva recovery chamber (12) via a discharge pipe (13); The skin fluid collection box (2) is internally installed with an illumination lamp (24) and a sound generator (22); a plurality of through holes (23) are provided on the side wall where the skin fluid collection box (2) and the saliva collection box (3) are in contact, for leeches to pass through; An induction liquid nozzle (34) is mounted on the side wall of the saliva collection box (3) for spraying the induction liquid. An electric slide rail (31) is mounted inside the saliva collection box (3). A retractable pressing mechanism (32) is detachably connected to the lower surface of the electric slide rail (31). The retractable pressing mechanism (32) comprises a sealing cover (323). A cylinder (321) is arranged inside the sealing cover (323). The cylinder (321) is slidably connected to a slide seat (324) on the inner wall of the sealing cover (323). The retractable end of the cylinder (321) passes through the sealing cover (323) and is connected to a pressing plate mechanism (33) for driving the pressing plate mechanism (33) to descend to contact the leech. A reciprocating pressing assembly (322) is mounted on the side wall of the sealing cover (323) for driving the pressing plate mechanism (33) to reciprocate to press the leech. The pressing plate mechanism (33) comprises a connecting vertical rod (331), a hollow main pressure plate (332) is fixed at the bottom of the connecting vertical rod (331), four sides of the hollow main pressure plate (332) are slidably connected with side scrapers (333), the side scrapers (333) and the bottom of the hollow main pressure plate (332) are located on the same plane, a scraper telescopic assembly (334) is arranged inside the hollow main pressure plate (332), a power-on assembly is installed on the surface of the cylinder (321), when the telescopic end of the cylinder (321) is retracted, the power-on assembly is energized, so that the scraper telescopic assembly (334) drives the side scrapers (333) to retract, and when the telescopic end of the cylinder (321) is extended, the power-on assembly is de-energized, so that the scraper telescopic assembly (334) drives the side scrapers (333) to extend; A spraying and flushing mechanism (4) is provided on the top of the saliva collection box (3); the spraying and flushing mechanism (4) extends to the skin fluid collection box (2) and the saliva collection box (3) respectively, and is used for flushing the collected skin fluid and saliva; A refrigeration unit (7) is provided on the back of the skin fluid collection box (2) and the saliva collection box (3). The refrigeration unit (7) is connected to a conducting pipe (8), and the conducting pipe (8) is communicated with the skin fluid collection box (2) and the saliva collection box (3) respectively.

2. The device for extracting leech salivary gland secretions according to claim 1, characterized in that: The spray flushing mechanism (4) comprises a spray main pipe (41) arranged at the top of the saliva collection box (3), a saliva flushing head (42) and a first skin liquid flushing head (43) arranged inside the saliva collection box (3), and a second skin liquid flushing head (44) arranged inside the skin liquid collection box (2); the saliva flushing head (42) is installed at the top of the saliva collection box (3), the second skin liquid flushing head (44) is installed on the upper side wall of the through hole (23), and the first skin liquid flushing head (43) is movably arranged on one side of the through hole (23) via a movable component.

3. The device for extracting leech salivary gland secretions according to claim 2, characterized in that: The movable assembly comprises a telescopic cylinder (5) fixed on the inner wall of the saliva collection box (3); a blocking plate (6) is fixed to the telescopic end of the telescopic cylinder (5); the first skin fluid flushing head (43) is mounted on the outer surface of the blocking plate (6); a plurality of nozzles matching the through holes (23) are arranged on the first skin fluid flushing head (43); the nozzles extend into the grooves on the surface of the blocking plate (6) and are used to flush the skin fluid inside the through holes (23).

4. The device for extracting leech salivary gland secretions according to claim 1, characterized in that: The reciprocating pressing assembly (322) comprises a motor (3221) fixed on the outer wall of the sealing cover (323); a driving end of the motor (3221) extends to the inner wall of the sealing cover (323) and is fixedly connected to a rotating disk (3222); a rotating rod (3223) is rotatably connected to the surface of the rotating disk (3222); a connecting block (3224) is fixed to the surface of the cylinder (321); and the connecting block (3224) is rotatably connected to the rotating rod (3223).

5. The device for extracting leech salivary gland secretions according to claim 1, characterized in that: The scraper telescopic assembly (334) is divided into four groups, which are respectively arranged on one side of the four side scrapers (333). The scraper telescopic assembly (334) includes a partition (3343) fixed inside the hollow main pressure plate (332), and the partition (3343) is connected to the side scraper (333) through a spring (3341). A first electromagnet block (3342) is fixed on the side wall of the partition (3343), and a second electromagnet block (3344) is fixed on the side wall of the side scraper (333).

6. The device for extracting leech salivary gland secretions according to claim 5, characterized in that: The power supply assembly comprises a first fixing plate (325) fixed to the bottom end of the shell of the cylinder (321) and a second fixing plate (326) fixed to the bottom of the telescopic end of the cylinder (321), a conductive groove (328) being fixed to the lower surface of the first fixing plate (325), and a conductive block (327) matching the conductive groove (328) being fixed to the upper surface of the second fixing plate (326).

7. The device for extracting leech salivary gland secretions according to claim 1, characterized in that: The top of the skin fluid collection box (2) is rotatably connected to a feed cover (21), the surface of the saliva collection box (3) is provided with an observation window and is rotatably connected to a discharge door, and one end of the discharge pipe (13) and the connecting pipe are both provided with a filter screen.

8. A method for extracting leech salivary gland secretions, according to the device for extracting leech salivary gland secretions according to any one of claims 1 to 7, characterized in that: The specific steps are as follows: S1, firstly placing a live leech into the skin fluid collection box (2), and at the same time starting the refrigeration unit (7) to lower the temperature inside the skin fluid collection box (2) and the saliva collection box (3), turning on the lighting (24) and the sound generator (22), and making use of the leech's preference for quietness and avoidance of light, allowing the leech to pass through the hole (23) and enter the dim saliva collection box (3). In the process of passing through the hole (23), the secretions of the leech's skin glands will be scraped off; S2, after the leech has completely entered the saliva collection box (3), the induction liquid is sprayed out through the induction liquid spray head (34) to feed the leech. While feeding the induction liquid, the telescopic rod (5) is extended to push the blocking plate (6) to move and block the through hole (23), and the skin gland secretions at the through hole (23) are flushed into the skin liquid recovery chamber (11) through the first skin liquid flushing head (43) and the second skin liquid flushing head (44); S3, after the induction liquid is fed, the cylinder (321) is started to move the pressing plate mechanism (33) downward to contact the leech at the bottom of the saliva collection box (3), and then the reciprocating pressing assembly (322) is started to press the leech multiple times to make it spit out the saliva secretions, and then the saliva secretions can be flushed into the saliva recovery chamber (12) through the saliva flushing head (42); S4. The body surface secretions and saliva secretions are discharged separately and filtered and collected, and the glycoproteins and polypeptide substances in the body surface secretions are processed and purified, and the saliva secretions are separated and purified according to conventional biochemical separation methods to obtain raw material products.

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