A screening system for zearalenone in cereals based on fluorescence imaging

Abstract

The present invention relates to a grain corn zearalenone screening system based on fluorescence imaging applied in the field of material analysis. A sticky film with multiple sticky layers is provided, and the sticky film is first drawn into a material box storing grain powder to be detected, and the grain powder is adhered to the sticky layer, and then introduced into a dark box to collect color fluorescence images. Compared with the prior art in which a single image of the upper surface of the grain powder in a sample cup is directly collected, the present application not only obtains multiple image data of the same sample by dispersing and adhering the grain powder in the material box to the surface of the sticky layer, and then sequentially collects multiple images. At the same time, because the sticky layer penetrates the inside of the grain powder for free adhesion, the multiple image data have sufficient randomness, effectively improving the accuracy of the color component of the grain powder to be detected obtained after taking the average value, thereby improving the accuracy of the inspection result.

Description

Technical Field

[0001] The present invention relates to a zearalenone screening system, and in particular to a grain zearalenone screening system based on fluorescence imaging applied in the field of material analysis. Background Art

[0002] Mycotoxins are secondary metabolites produced by fungi, mainly including aflatoxins, zearalenone, deoxynivalenol, etc. Rapid detection methods for zearalenone, such as ELISA, colloidal gold test paper, immunoaffinity columns, etc., can improve detection speed and accuracy. These detection methods require extraction and purification with organic solvents such as acetonitrile or methanol, and the steps are cumbersome and time-consuming.

[0003] The specification of Chinese patent CN201810628805.9 discloses a system and method for rapid visualization screening of zearalenone by fluorescence imaging, which specifically includes: pre-processing samples, instrument debugging, collecting fluorescent color images, determining the zearalenone content by high performance liquid chromatography, establishing and optimizing mathematical models, and determining the zearalenone content. The light source modules of the present invention are arranged at equal intervals and densely, and each sequence of LEDs is independently controlled to increase the uniformity and stability of illumination, thereby improving detection speed and prediction accuracy.

[0004] However, the above-mentioned process of collecting the fluorescent color image of the sample to be tested is to place the sample to be tested in a sample cup, and then collect the image of the upper surface of the sample to be tested. The situation inside the sample cannot be collected, and there is a defect of incomplete collection, which easily leads to a situation where the accuracy of the test result is low. Summary of the invention

[0005] In view of the above-mentioned prior art, the technical problem to be solved by the present invention is that the existing method of placing the sample to be tested in a sample cup and capturing an image of its upper surface is prone to incomplete collection, resulting in low accuracy of the test result, because the distribution of the internal situation of the sample cannot be captured.

[0006] In order to solve the above problems, the present invention provides a corn zearalenone screening system based on fluorescence imaging, comprising a dark box, a light intensity control module and a computer terminal arranged outside the dark box, wherein a camera, an annular light source module and a photoelectric detector are arranged on the dark box from top to bottom in sequence, the camera is fixedly connected to the top of the dark box, and its lens extends into the dark box, the annular light source module is located inside the dark box, and a pair of electric push rods are fixedly connected between the upper end of the annular light source module and the top of the dark box, the photoelectric detector is fixedly connected to the inner bottom surface of the dark box and is located in the uniform illumination area of ​​the annular light source module, the annular light source module and the photoelectric detector are both electrically connected to the light intensity control module, the camera and the light intensity control module are both electrically connected to the computer terminal, a material box for placing grain powder to be detected is provided on one side of the dark box, and the lower ends of the dark box and the material box are fixedly connected to the same base;

[0007] A unwinding roller is connected to the side end of the dark box, a main guide roller is provided on the upper side of the material box, a secondary guide roller and a winding roller are provided on the side of the dark box away from the material box, one end of the winding roller is fixedly connected to the output end of the motor, the motor is electrically connected to the computer terminal, the outer end of the unwinding roller is fixedly connected to one end of the adhesive film, the other end of the adhesive film first bypasses the inside of the adhesive film, the outer end of the main guide roller then penetrates one side end of the dark box into the inside, and penetrates the other side end of the dark box, bypasses the outer end of the secondary guide roller until it is fixedly connected to the outer end of the winding roller, the adhesive film is in a normally straightened state and one end of it is wrapped and wound around the outer end of the unwinding roller, and a pair of side ends on the dark box connected to the adhesive film are fixedly connected to a shading assembly.

[0008] As a further supplement to the present application, a pair of side ends of the dark box are each provided with perforations, and the adhesive film movably passes through the inside of the pair of perforations.

[0009] As a further supplement to the present application, the adhesive film includes a soft film tape, one end surface of which is coated with a plurality of evenly distributed adhesive layers, and when the soft film tape is rolled around a unwinding roller, the adhesive layer is located on the inner side in the rolling direction.

[0010] As a further supplement to the present application, the soft film tape includes a plastic film and a release film, and the release film is fixedly connected to the end surface of the plastic film away from the adhesive layer.

[0011] As another improvement of the present application, the shading assembly includes a top seat, a cylinder, a side panel and a pad which are distributed in sequence from top to bottom and fixedly connected to the side end of the dark box. An inner groove is provided at the lower end of the side panel, and a pressure plate is slidably connected inside the inner groove. The upper end of the cylinder is fixedly connected to the lower end of the top seat, and the telescopic end of the cylinder moves downward through the upper end of the side panel until it is fixedly connected to the upper end of the pressure plate. The cylinder is electrically connected to the computer terminal.

[0012] As another improved supplement of the present application, the top seat, cylinder and side plate are all located on the upper side of the perforation, the pad is located on the lower side of the perforation and the two are flush, and a rubber pad is fixedly connected to the lower end of the pressure plate, and the length of the pressure plate and the rubber pad is greater than the length of the slot of the perforation.

[0013] As a further supplement to the present application, the material box includes a box body and a round rod fixedly connected to the inside of the box body, and the round rod is located in the middle and lower area of ​​the inside of the box body.

[0014] As a further supplement to the present application, a pair of mounting plates are provided on the outer end rotating sleeve of the unwinding roller, the adhesive film is located between the pair of mounting plates, the mounting plates are fixedly connected to the side ends of the dark box, and the unwinding roller is located between the dark box and the main roller in the horizontal direction.

[0015] As a further supplement to the present application, a pair of auxiliary vertical plates and a pair of main vertical plates are fixedly connected to the upper end of the base, the auxiliary guide roller is fixedly connected between the pair of auxiliary vertical plates, the two ends of the winding roller respectively pass through a pair of auxiliary vertical plates and are rotatably connected to the inside thereof, a pair of main vertical plates are respectively located on both sides of the material box, and the main guide roller is fixedly connected between the pair of main vertical plates.

[0016] A system for screening zearalenone in grain corn based on fluorescence imaging, the method for using the system comprises the following steps:

[0017] S1. According to the prior art, the luminous intensity of the ring light source module is adjusted, and the acquisition parameters of the camera are standardized;

[0018] S2, adding the processed grain powder to be tested into the material box, starting the motor through the computer terminal, so that the winding roller reels the sticky film, and the unwinding roller unwinds the sticky film, so that the sticky film slowly passes through the grain powder to be tested, and the sticky layer adheres to the grain powder to be tested;

[0019] S3. When the sticky material layer with the grain powder to be detected moves to the central area inside the dark box, the motor is turned off, the winding is stopped, and then the cylinder is started through the computer terminal to make the light shielding component close the perforation, and the three sequence LED light source modules on the ring light source module are sequentially lit to excite the grain powder to be detected, and the fluorescent image of the grain powder to be detected on the sticky material layer is collected through the camera, and then the R, G and B color components of the three collected fluorescent color images are respectively extracted;

[0020] S4, after the grain powder to be detected on a single adhesive layer is collected, the cylinder is started to open the perforation, and the motor is started again to make the adhesive film continue to move until the next adhesive layer adsorbing the grain powder to be detected reaches the central area inside the dark box, and step S3 is performed again;

[0021] S5. Perform step S4 multiple times, obtain the R, G and B color components of multiple image data for the same grain powder to be detected, take the average value as the color component of the grain powder to be detected, and then use the zearalenone mathematical model established by optimizing the existing technology to calculate the zearalenone content at each spatial site of the color image.

[0022] In summary, the present application sets a sticky film with multiple sticky layers, and makes it enter the material box storing the grain powder to be tested in a traction manner, and then introduces it into a dark box to collect color fluorescent images after the grain powder is adhered to the grain powder through the sticky layer. Compared with the prior art that directly collects a single image of the upper surface of the grain powder in the sample cup, the present application disperses and adheres the grain powder in the material box to the surface of the sticky layer, and then collects multiple images in sequence. Not only does it obtain multiple image data of the same sample, but also because the sticky layer penetrates the inside of the grain powder for free adhesion, the multiple image data have sufficient randomness, which effectively improves the accuracy of the color component of the grain powder to be tested obtained after taking the average value, thereby improving the accuracy of the inspection result. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 The three-dimensional embodiment of the first and second embodiments of this application Figure 1 ;

[0024] Figure 2 The three-dimensional embodiment of the first and second embodiments of this application Figure 2 ;

[0025] Figure 3 The three-dimensional embodiment of the first and second embodiments of this application Figure 3 ;

[0026] Figure 4 The side structure diagram of the first and second embodiments of the present application is shown in FIG. Figure 1 ;

[0027] Figure 5 for Figure 4 The structural diagram at A in the middle;

[0028] Figure 6 It is a schematic diagram of the partial side structure of the adhesive film of the first and second embodiments of the present application;

[0029] Figure 7 The side structure diagram of the first and second embodiments of the present application is shown in FIG. Figure 2 ;

[0030] Figure 8 The side structure diagram of the first and second embodiments of the present application is shown in FIG. Figure 3 ;

[0031] Fig. 9The side structure diagram of the first and second embodiments of the present application is shown in FIG. Figure 4 .

[0032] Description of the numbers in the figure:

[0033] 1 dark box, 101 perforation, 2 camera, 3 ring light source module, 4 photoelectric detector, 5 electric push rod, 6 base, 7 adhesive film, 71 soft film tape, 7101 plastic film, 7102 release film, 72 adhesive layer, 8 material box, 81 box body, 82 round rod, 9 unwinding roller, 901 mounting plate, 10 main guide roller, 11 auxiliary guide roller, 12 winding roller, 13 motor, 14 auxiliary vertical plate, 15 main vertical plate, 1601 top seat, 1602 cylinder, 1603 side plate, 1604 pad, 1605 pressure plate, 1606 rubber pad. DETAILED DESCRIPTION

[0034] Two implementation modes of the present application are described in detail below with reference to the accompanying drawings.

[0035] The first implementation method:

[0036] The present invention provides a system for screening zearalenone in grains based on fluorescence imaging. Figure 1 , including a dark box 1, a light intensity control module and a computer terminal arranged outside the dark box 1, wherein a camera 2, a ring light source module 3 and a photoelectric detector 4 are arranged on the dark box 1 from top to bottom, the camera 2 is fixedly connected to the top of the dark box 1, and its lens extends into the inside of the dark box 1, the ring light source module 3 is located inside the dark box 1, and a pair of electric push rods 5 are fixedly connected between its upper end and the top of the dark box 1, the electric push rods 5 are used to adjust the upper and lower positions of the ring light source module 3, the photoelectric detector 4 is fixedly connected to the inner bottom surface of the dark box 1 and is located in the uniform illumination area of ​​the ring light source module 3, the ring light source module 3 and the photoelectric detector 4 are both electrically connected to the light intensity control module, the light intensity control module adjusts the illumination intensity of the ring light source module 3 according to the feedback signal of the photoelectric detector 4, the camera 2 and the light intensity control module are both electrically connected to the computer terminal, and the above structures are all prior arts.

[0037] See also Figure 1 and Figure 3A material box 8 for placing the grain powder to be tested is provided on one side of the dark box 1. The lower ends of the dark box 1 and the material box 8 are fixedly connected to the same base 6. A unwinding roller 9 is connected to the side end of the dark box 1. A main roller 10 is provided on the upper side of the material box 8. A secondary guide roller 11 and a winding roller 12 are provided on the side of the dark box 1 away from the material box 8. One end of the winding roller 12 is fixedly connected to the output end of the motor 13. The lower end of the motor 13 is fixedly connected to the upper end of the base 6 through a bracket. The motor 13 is electrically connected to the computer terminal. The outer end of the unwinding roller 9 is fixedly connected to one end of the adhesive film 7. The other end of the adhesive film 7 first bypasses the interior of the adhesive film 7 and the outer end of the main roller 10 in turn, and then passes through one side end of the dark box 1 to enter its interior, and passes through the other side end of the dark box 1, bypasses the outer end of the secondary guide roller 11 until it is fixedly connected to the outer end of the winding roller 12. The adhesive film 7 is in a normal straightened state and one end of it is wound and rolled around the outer end of the unwinding roller 9.

[0038] Combination Figure 4 and Figure 5 A pair of side ends of the dark box 1 are each provided with perforations 101, and the adhesive film 7 is movable through the pair of perforations 101, that is, the adhesive film 7 is introduced into the dark box 1 from the perforations 101 on one side, and then led out of the dark box 1 through the perforations 101 on the other side.

[0039] See also Figure 6 The adhesive film 7 includes a soft film strip 71, one end surface of which is coated with a plurality of evenly distributed adhesive layers 72. Figure 7 and Figure 8 As shown, when the adhesive film 7 passes through the interior of the cereal powder in the material box 8, the cereal powder will adhere to the adhesive layer 72, so after the adhesive layer 72 is removed from the material box 8, a uniform and thin layer of cereal powder will adhere to its surface;

[0040] See also Figure 7 The material box 8 includes a box body 81 and a round rod 82 fixedly connected to the inside of the box body 81. The round rod 82 is located in the middle and lower area of ​​the box body 81. After the adhesive film 7 enters the box body 81, it goes around the lower surface of the round rod 82 and then moves upward. Figure 8 As shown, when the soft film belt 71 is wound around the unwinding roller 9, the adhesive layer 72 is located on the inner side of the winding direction, so that the adhesive layer 72 passes through the round rod 82 and the main roller 10 in turn, and enters the interior of the dark box 1 with the front side facing up, which is convenient for the camera 2 to capture the fluorescent image of the adhered grain powder. In addition, by arranging the round rod 82, the main roller 10, and the auxiliary guide roller 11 on the side of the soft film belt 71 away from the adhesive layer 72, the contact traction is performed with the end face without the adhesive layer 72, so that the grain powder adhered to the adhesive layer 72 is not easily dropped due to friction, thereby improving the effectiveness and accuracy of image acquisition.

[0041] Additional explanation: If Fig. 9 As shown, a sufficient spacing needs to be set between adjacent adhesive layers 72. The specific spacing size can be set according to the spacing size between a pair of perforations 101, so that when one of the adhesive layers 72 is located in the central area inside the dark box 1 for image acquisition, the adjacent adhesive layers 72 on both sides are located outside the dark box 1, which is not easy to interfere with the image acquisition of the grain powder on the adhesive layer 72 in the dark box 1.

[0042] See also Figure 6 The soft film tape 71 includes a plastic film 7101 and a release film 7102. The release film 7102 is fixedly connected to the end surface of the plastic film 7101 away from the adhesive layer 72. Since the adhesive layer 72 will contact the other end surface of the soft film tape 71 when winding, the release film 7102 is set to prevent the adhesive layer 72 from being easily adhered to the soft film tape 71 before use.

[0043] See also Figure 2 A pair of mounting plates 901 are rotatably sleeved on the outer end of the unwinding roller 9, the adhesive film 7 is located between the pair of mounting plates 901, the mounting plate 901 is fixedly connected to the side end of the dark box 1, and the unwinding roller 9 is fixed to the side end of the dark box 1 through the mounting plate 901. In the horizontal direction, the unwinding roller 9 is located between the dark box 1 and the main roller 10, which is convenient for introducing the adhesive layer 72 into the dark box 1 with the front side facing up.

[0044] See also Figure 2 and Figure 3 A pair of auxiliary vertical plates 14 and a pair of main vertical plates 15 are fixedly connected to the upper end of the base 6, the auxiliary guide roller 11 is fixedly connected between the pair of auxiliary vertical plates 14, the two ends of the winding roller 12 respectively pass through the pair of auxiliary vertical plates 14 and are rotatably connected to the inside thereof, the auxiliary vertical plates 14 play a positioning role for the auxiliary guide roller 11 and the winding roller 12, a pair of main vertical plates 15 are respectively located on both sides of the material box 8, the main guide roller 10 is fixedly connected between the pair of main vertical plates 15, and the main vertical plates 15 play a positioning role for the main guide roller 10.

[0045] A system for screening zearalenone in grain corn based on fluorescence imaging, the method for using the system comprises the following steps:

[0046] S1. According to the prior art, the luminous intensity of the annular light source module 3 is adjusted, and the acquisition parameters of the camera 2 are standardized;

[0047] S2, adding the processed grain powder to be tested into the material box 8, starting the motor 13 through the computer terminal, so that the winding roller 12 winds up the adhesive film 7, and the unwinding roller 9 unwinds the adhesive film 7, so that the adhesive film 7 slowly passes through the grain powder to be tested, and the adhesive layer 72 adheres to the grain powder to be tested;

[0048] S3, when the sticky material layer 72 with the grain powder to be detected is moved to the inner central area of ​​the dark box 1, the motor 13 is turned off, the winding is stopped, and the perforation 101 is shielded (so that the external light is not easy to enter the dark box 1), the three sequence LED light source modules on the ring light source module 3 are sequentially lit to excite the grain powder to be detected, and the fluorescent image of the grain powder to be detected on the sticky material layer 72 is collected by the camera 2, and then the R, G and B color components of the three collected fluorescent color images are respectively extracted;

[0049] S4, after the grain powder to be detected on a single adhesive layer 72 is collected, the motor 13 is started again to make the adhesive film 7 continue to move until the next adhesive layer 72 adsorbing the grain powder to be detected reaches the central area inside the dark box 1, and step S3 is performed again;

[0050] S5. Perform step S4 multiple times, obtain the R, G and B color components of multiple image data for the same grain powder to be detected, take the average value as the color component of the grain powder to be detected, and then use the zearalenone mathematical model established by optimizing the existing technology to calculate the zearalenone content at each spatial site of the color image.

[0051] By providing an adhesive film 7 with multiple adhesive layers 72, and making it enter the material box 8 storing the grain powder to be detected in a traction manner, after the grain powder is adhered by the adhesive layer 72, it is introduced into the dark box 1 to collect color fluorescence images. Compared with the prior art that directly collects a single image of the upper surface of the grain powder in the sample cup, the present application not only obtains multiple image data of the same sample by dispersing and adhering the grain powder in the material box 8 to the surface of the adhesive layer 72, and then collects multiple images in sequence, but also because the adhesive layer 72 penetrates the inside of the grain powder for free adhesion, the multiple image data have sufficient randomness, which effectively improves the accuracy of the color component of the grain powder to be detected obtained after taking the average value, thereby improving the accuracy of the inspection result.

[0052] Second implementation method:

[0053] This embodiment is based on the embodiment 1 and is additionally provided with a shading component for shading the through hole 101. The details are as follows: Figure 3 , Figure 4 and Figure 5A pair of side ends on the dark box 1 connected to the adhesive film 7 are fixedly connected with a shading assembly, and the shading assembly includes a top seat 1601, a cylinder 1602, a side plate 1603 and a pad 1604 which are distributed from top to bottom and fixedly connected to the side ends of the dark box 1. An inner groove is provided at the lower end of the side plate 1603, and a pressure plate 1605 is slidably connected inside the inner groove. The upper end of the cylinder 1602 is fixedly connected to the lower end of the top seat 1601, and the telescopic end of the cylinder 1602 moves downward to penetrate the upper end of the side plate 1603 until it is fixedly connected to the upper end of the pressure plate 1605, and the cylinder 1602 is electrically connected to the computer terminal.

[0054] The top seat 1601, the cylinder 1602 and the side plate 1603 are all located on the upper side of the through hole 101, the pad 1604 is located on the lower side of the through hole 101 and the two are flush, and the lower end of the pressure plate 1605 is fixedly connected with a rubber pad 1606, and the length of the pressure plate 1605 and the rubber pad 1606 are greater than the length of the slot of the through hole 101.

[0055] Through the above arrangement, the shading component can be used to perform shading treatment on the perforation 101 in step S3: the cylinder 1602 is started through the computer terminal, and the cylinder 1602 is extended to push the pressing plate 1605 and the rubber pad 1606 downward until the rubber pad 1606 is pressed tightly against the upper end of the pad 1604 (because the soft film belt 71 is thin and the rubber pad 1606 has a certain elastic deformation ability, the rubber pad 1606 and the pad 1604 can be fitted together), closing the opening of the perforation 101, so that it is not easy for external light to enter the dark box 1 through the perforation 101, and it is not easy to affect the acquisition quality of the fluorescent image;

[0056] In step S4 , before starting the motor 13 , the cylinder 1602 is shortened to move the pressing plate 1605 and the rubber pad 1606 back into the inner groove to release the pressing of the soft film belt 71 , and then the motor 13 is started again to continue pulling the adhesive film 7 .

[0057] In view of current practical needs, the above-mentioned implementation mode adopted in this application is not limited to the scope of protection. Various changes made within the knowledge scope of technical personnel in this field without departing from the concept of this application still fall within the scope of protection of the present invention.

Claims

1. A corn zearalenone screening system based on fluorescence imaging, comprising a dark box (1), a light intensity control module and a computer terminal arranged outside the dark box (1), wherein the dark box (1) is provided with a camera (2), an annular light source module (3) and a photodetector (4) in order from top to bottom, wherein the camera (2) is fixedly connected to the top of the dark box (1), and its lens extends into the interior of the dark box (1), the annular light source module (3) is located inside the dark box (1), and a pair of electric push rods (5) are fixedly connected between the upper end of the annular light source module and the top of the dark box (1), the photodetector (4) is fixedly connected to the inner bottom surface of the dark box (1) and is located in the uniform illumination area of ​​the annular light source module (3), the annular light source module (3) and the photodetector (4) are both electrically connected to the light intensity control module, and the camera (2) and the light intensity control module are both electrically connected to the computer terminal, characterized in that: A material box (8) for placing the grain powder to be tested is provided on one side of the dark box (1), and the lower ends of the dark box (1) and the material box (8) are fixedly connected to the same base (6); The side end of the dark box (1) is connected to a reeling roller (9), the upper side of the material box (8) is provided with a main guide roller (10), the side of the dark box (1) away from the material box (8) is provided with a secondary guide roller (11) and a reeling roller (12), one end of the reeling roller (12) is fixedly connected to the output end of the motor (13), the motor (13) is electrically connected to the computer terminal, the outer end of the reeling roller (9) is fixedly connected to one end of the adhesive film (7), the adhesive film (7 ) first passes through the inside of the adhesive film (7), the outer end of the main guide roller (10), and then passes through one side end of the dark box (1) into the inside thereof, and passes through the other side end of the dark box (1), passes through the outer end of the auxiliary guide roller (11) until it is fixedly connected to the outer end of the winding roller (12). The adhesive film (7) is in a normally straightened state and one end thereof is wound and wound around the outer end of the unwinding roller (9). A pair of side ends of the dark box (1) connected to the adhesive film (7) are fixedly connected to a light-shielding component. A pair of side ends of the dark box (1) are each provided with a perforation (101), and the adhesive film (7) is movable through the inside of the pair of perforations (101); The adhesive film (7) includes a soft film strip (71), one end surface of the soft film strip (71) is coated with a plurality of evenly distributed adhesive layers (72), when the soft film strip (71) is wound around the unwinding roller (9), the adhesive layer (72) is located on the inner side of the winding direction, and the soft film strip (71) includes a plastic film (7101) and a release film (7102), and the release film (7102) is fixedly connected to the end surface of the plastic film (7101) away from the adhesive layer (72); The shading assembly comprises a top seat (1601), a cylinder (1602), a side plate (1603) and a pad (1604) which are sequentially distributed from top to bottom and fixedly connected to the side end of the dark box (1); an inner groove is provided at the lower end of the side plate (1603); a pressure plate (1605) is slidably connected to the interior of the inner groove; the upper end of the cylinder (1602) is fixedly connected to the lower end of the top seat (1601); the telescopic end of the cylinder (1602) moves downward through the upper end of the side plate (1603) until it is fixedly connected to the upper end of the pressure plate (1605); and the cylinder (1602) is electrically connected to a computer terminal; The top seat (1601), the cylinder (1602) and the side plate (1603) are all located on the upper side of the perforation (101); the pad (1604) is located on the lower side of the perforation (101) and the two are flush; the lower end of the pressing plate (1605) is fixedly connected to a rubber pad (1606); the lengths of the pressing plate (1605) and the rubber pad (1606) are greater than the length of the notch of the perforation (101); The material box (8) comprises a box body (81) and a round rod (82) fixedly connected to the inside of the box body (81), wherein the round rod (82) is located in the middle and lower area inside the box body (81); The outer end of the unwinding roller (9) is rotatably sleeved with a pair of mounting plates (901), the adhesive film (7) is located between the pair of mounting plates (901), the mounting plates (901) are fixedly connected to the side ends of the dark box (1), and the unwinding roller (9) is located between the dark box (1) and the main roller (10) in the horizontal direction; The upper end of the base (6) is fixedly connected to a pair of auxiliary vertical plates (14) and a pair of main vertical plates (15); the auxiliary guide roller (11) is fixedly connected between the pair of auxiliary vertical plates (14); both ends of the winding roller (12) respectively pass through the pair of auxiliary vertical plates (14) and are rotatably connected to the inside thereof; the pair of main vertical plates (15) are respectively located on both sides of the material box (8); and the main guide roller (10) is fixedly connected between the pair of main vertical plates (15); The method for using the above-mentioned fluorescence imaging-based zearalenone screening system for grain corn includes the following steps: S1. Adjusting the luminous intensity of the annular light source module (3) and standardizing the acquisition parameters of the camera (2) according to the existing technology; S2, adding the processed grain powder to be tested into the material box (8), starting the motor (13) through the computer terminal, causing the winding roller (12) to rewind the adhesive film (7), and the unwinding roller (9) to unwind the adhesive film (7), so that the adhesive film (7) slowly passes through the grain powder to be tested, and the adhesive layer (72) adheres to the grain powder to be tested; S3. When the sticky material layer (72) with the grain powder to be detected is moved to the center area inside the dark box (1), the motor (13) is turned off, the winding is suspended, and then the cylinder (1602) is started through the computer terminal to make the light shielding component close the perforation (101), and the three sequence LED light source modules on the ring light source module (3) are sequentially illuminated to excite the grain powder to be detected, and the fluorescence image of the grain powder to be detected on the sticky material layer (72) is collected through the camera (2), and then the R, G and B color components of the three collected fluorescence color images are respectively extracted; S4, after the grain powder to be tested on a single adhesive layer (72) is collected, the cylinder (1602) is started to open the perforation (101), and the motor (13) is started again to make the adhesive film (7) continue to move until the next adhesive layer (72) adsorbed with the grain powder to be tested reaches the central area inside the dark box (1), and step S3 is performed again; S5. Perform step S4 multiple times. For the same grain powder to be tested, obtain the R, G, and B color components of multiple image data, take the average value as the color component of the grain powder to be tested, and then use the zearalenone mathematical model optimized and established by existing technology to calculate the zearalenone content at each spatial site of the color image.

Images