Application of a plant-derived Chinese medicine combination extract in regulating plant growth

Abstract

The present invention discloses an application of a plant-derived Chinese medicine combination extract in regulating plant growth, and belongs to the technical field of plant growth regulation. The plant-derived Chinese medicine combination extract is a salvia miltiorrhiza and safflower composite polysaccharide; the regulating plant growth includes alleviating crop damage caused by abiotic stress and improving crop stress resistance. The salvia miltiorrhiza and safflower composite polysaccharide is prepared by mixing salvia miltiorrhiza and safflower as raw materials in a mass ratio of 3:1 and extracting the polysaccharide. The use concentration range of the salvia miltiorrhiza-safflower composite polysaccharide is 1~500 ng / mL. The present invention prepares a composite polysaccharide with salvia miltiorrhiza and safflower to regulate the growth of plants under adversity, which can not only promote the germination and growth of seeds under drought stress, and significantly improve the nutritional content and yield of plants; it can also promote the growth and development of crops under low temperature stress, and can be used as a plant growth regulator or foliar fertilizer on crops.

Description

Technical Field

[0001] The invention relates to the technical field of plant growth regulation, and in particular to application of a plant-derived Chinese medicine combination extract in regulating plant growth. Background Art

[0002] Plant stress resistance, also known as plant resistance, is the ability of plants to resist adverse factors. There are two main types of adversity, namely abiotic stress (high temperature stress, low temperature stress, drought stress, etc.) and biotic stress (disease, insect pests, weed damage, etc.). Adverse conditions can easily cause plant cell dehydration, which can easily lead to imbalance of active oxygen metabolism and cell membrane damage in plants, resulting in damage to the photosynthetic system, decreased photosynthesis in plants, and wilting of plants. Therefore, applying certain exogenous components to plants under adverse conditions is one of the effective measures to improve plant resistance.

[0003] Plant polysaccharides, also known as plant polysaccharides, refer to products containing more than 10 sugar groups in plant extracts and connected by α / β-glycosidic bonds. They have multiple biological functions such as antioxidant, antibacterial and immunomodulatory. Polysaccharides with diverse structures from natural sources have rich biological activities and play an important role in ensuring the normal operation of life organisms. They are important life substances and have broad prospects in the research and development of health foods and functional foods. When plants are subjected to abiotic stresses such as high temperature, low temperature, and drought, they are very likely to cause imbalance in the metabolism of active oxygen and damage to cell membranes in the plant body, leading to damage to the photosynthetic system and causing plant wilting. At present, traditional fertilizers in the agricultural field release nutrients quickly and have low efficacy. Therefore, foreign scholars have studied fertilizer coatings based on polysaccharide components, which can not only effectively improve fertilizer utilization, but also improve the disadvantages of conventional non-degradable coatings. As fertilizer additives, plant polysaccharides can inhibit soil nitrification to a certain extent, and can also increase crop yields and nitrogen fertilizer utilization. Applying a certain amount of polysaccharide foliar fertilizer to plant seeds and young shoots can promote seed germination and growth, and significantly improve the nutritional content and yield of plants. Although different plant polysaccharides have antioxidant, antibacterial and immunomodulatory effects, the components contained in different plant polysaccharides are not exactly the same, and their effects are not exactly the same and there are differences. For example, the foliar use of brown algae oligosaccharides can improve the growth and quality of sweet cherries; as a blended fertilizer applied to rice soil, it can increase the number of effective panicles, the number of panicles and the fruiting rate of rice, and can also improve the utilization rate of fertilizer. Field spraying of Stropharia polysaccharides on kiwifruit under high temperature stress increased peroxidase activity, improved photosynthesis, improved kiwifruit's ability to resist high temperatures, and promoted plant growth. The patent with application number CN202210689619.2 discloses a composite polysaccharide of salvia miltiorrhiza and safflower, its preparation method and application. Extracting polysaccharides from salvia miltiorrhiza and safflower together can synergistically promote its antioxidant capacity. However, good antioxidant capacity does not mean that the stress resistance of crops can be improved. There is no report on the use of composite polysaccharides in crop stress resistance research. Summary of the invention

[0004] In view of the above-mentioned prior art, the purpose of the present invention is to provide an application of a plant-derived Chinese medicine combination extract in regulating plant growth. The present invention prepares a composite polysaccharide with Salvia miltiorrhiza and Carthamus tinctorius to regulate the growth of plants under adverse conditions, which can not only promote the germination and growth of seeds under drought stress, and significantly improve the nutritional content and yield of plants; it can also promote the growth and development of crops under low temperature stress, and can act on crops as a plant growth regulator or foliar fertilizer.

[0005] To achieve the above object, the present invention adopts the following technical solution:

[0006] In a first aspect of the present invention, a use of a plant-derived Chinese medicine combination extract in regulating plant growth is provided, wherein the plant-derived Chinese medicine combination extract is a salvia miltiorrhiza and safflower complex polysaccharide;

[0007] The regulating plant growth includes alleviating the damage of crops caused by abiotic stress and improving the stress resistance of crops.

[0008] The salvia miltiorrhiza and safflower composite polysaccharide is prepared by mixing salvia miltiorrhiza and safflower as raw materials in a mass ratio of (10-1): (1-10) and extracting the polysaccharide.

[0009] Preferably, the mass ratio of Salvia miltiorrhiza to Carthamus tinctorius is 3:1.

[0010] Preferably, the abiotic stress is drought stress, and the stress resistance is germination of crop seeds and growth of seedlings under drought stress.

[0011] Preferably, the concentration of the Salvia miltiorrhiza-Safflower complex polysaccharide is 1-500 ng / mL.

[0012] Preferably, the concentration of the Salvia miltiorrhiza-Safflower complex polysaccharide is 10 ng / mL.

[0013] Preferably, the abiotic stress is low temperature stress, and the stress resistance is the growth and development of crops under low temperature stress.

[0014] Preferably, the concentration of the Salvia miltiorrhiza-Safflower complex polysaccharide is 10-500 ng / mL.

[0015] Preferably, the concentration of the Salvia miltiorrhiza-Safflower complex polysaccharide is 20 ng / mL.

[0016] Regulating plant growth is to promote the germination of crop seeds and seedling growth under drought stress, or to promote the growth and development of crops under low temperature stress.

[0017] The second aspect of the present invention provides a plant growth regulating product, which is a plant growth regulator or foliar fertilizer, and the product contains salvia miltiorrhiza and safflower complex polysaccharide as an effective ingredient; the salvia miltiorrhiza and safflower complex polysaccharide is prepared by using salvia miltiorrhiza and safflower as raw materials, mixing them in a mass ratio of (10~1):(1~10), and adopting polysaccharide extraction.

[0018] Beneficial effects of the present invention:

[0019] (1) The salvia miltiorrhiza-safflower composite polysaccharide of the present invention is derived from natural plant polysaccharides and has broad application prospects. Compared with chemical synthetic agents, the salvia miltiorrhiza-safflower composite polysaccharide components are safer and have fewer side effects. When used as plant growth regulators or foliar fertilizers on crops, they can effectively activate the plant's defense mechanism, alleviate crop damage caused by abiotic stress, enhance stress resistance, and improve crop quality.

[0020] (2) Seed soaking with the Salvia miltiorrhiza-Safflower composite polysaccharide solution of the present invention can effectively promote the germination of wheat seeds and the growth of wheat seedlings under drought stress; the test results on tomato resistance to chilling damage show that spraying a certain concentration of composite polysaccharide solution can improve plant photosynthesis and improve the inhibitory effect of chilling damage on tomato plant growth. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 :Effects of different treatments on wheat seed germination under drought stress;

[0022] Figure 2 :Effects of different treatments on the growth of wheat seedlings under drought stress;

[0023] Figure 3 : Top view of tomato growth status of each group of treatments on the second day after returning to normal temperature;

[0024] Figure 4 : Overall picture of tomato growth status of each group of treatments on the second day after returning to normal temperature;

[0025] Figure 5 : The effects of the treatments of the embodiment group and the comparative examples 1 to 3 on wheat seeds under drought stress;

[0026] Figure 6 : Effects of the treatments of the embodiment group and the comparative examples 1 to 3 on the growth of tomato seedlings under low temperature stress;

[0027] Figure 7 : Infrared spectra of polysaccharides prepared in Examples and Comparative Examples 1 to 3, wavelength range 500 to 4000 cm -1 ;

[0028] Figure 8 : Infrared spectra of polysaccharides prepared in Examples and Comparative Examples 1 to 3, wavelength range 400 to 2000 cm-1 ;

[0029] Fig. 9 : Molecular weight distribution diagrams of the polysaccharides prepared in Example and Comparative Examples 1-2, wherein (a) is the molecular weight distribution diagram of salvia miltiorrhiza polysaccharide, (b) is the molecular weight distribution diagram of safflower polysaccharide, and (c) is the molecular weight distribution diagram of salvia miltiorrhiza and safflower composite polysaccharide. DETAILED DESCRIPTION

[0030] It should be noted that the following detailed descriptions are illustrative and are intended to provide further explanation of the present application. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which the present application belongs.

[0031] As mentioned in the background technology, although it has been reported that extracting polysaccharides from salvia miltiorrhiza and safflower together can synergistically promote their antioxidant capacity, good antioxidant capacity does not mean that it can improve the stress resistance of crops, and there has been no report on the use of salvia miltiorrhiza and safflower composite polysaccharides in crop stress resistance research.

[0032] Based on this, the present invention provides an application of a plant-derived Chinese medicine combination extract in regulating plant growth. After studying plant-derived Chinese medicine, the inventors conducted experiments on single plant-derived Chinese medicine polysaccharides, mixed plant-derived Chinese medicine polysaccharides and composite plant-derived Chinese medicine polysaccharides, and found that the composite polysaccharide prepared by using salvia miltiorrhiza and safflower in a mass ratio of 3:1 is significantly better than the composite polysaccharides prepared by using single plant-derived Chinese medicine polysaccharides, mixed plant-derived Chinese medicine polysaccharides and other ratios in alleviating crop damage caused by abiotic stress and improving stress resistance. The inventors also conducted experiments on composite polysaccharides of safflower with different concentrations. When the concentration of salvia miltiorrhiza-safflower composite polysaccharides is 10 ng / mL, it can improve the ability of wheat seedlings to resist drought stress, and when the concentration is 20 ng / mL, it can improve the ability of tomato seedlings to resist low temperature stress.

[0033] In order to enable those skilled in the art to more clearly understand the technical solution of the present application, the technical solution of the present application will be described in detail below in conjunction with specific embodiments.

[0034] The test materials used in the examples of the present invention are all conventional test materials in the art and can be purchased through commercial channels.

[0035] Example: Preparation of Salvia miltiorrhiza-Safflower Complex Polysaccharide

[0036] Take 3 g of Danshen and 1 g of Carthamus tinctorius, crush them and pass through a 40-mesh sieve; add 400 g of deionized water; perform ultrasonic-assisted extraction of polysaccharides, the ultrasonic frequency is 30KHz, the power is 150 W, and the ultrasonic time is 30 min. After extraction, centrifuge and filter to obtain a water extract, and add sevage reagent (n-butanol: chloroform in a volume ratio of 1:4) to the water extract to remove protein. The mass ratio of water extract to sevage reagent is 4:1. Take the upper aqueous phase and add ethanol to make the final ethanol concentration about 70wt%, refrigerate and stand at 4℃, filter after 24 hours, and freeze-dry at -30℃ for 48 hours to obtain Danshen Carthamus tinctorius composite polysaccharide (denoted as 3S1C).

[0037] Comparative Example 1

[0038] The difference from the embodiment is that no Salvia miltiorrhiza was added, and safflower polysaccharide (denoted as CTPS) was finally obtained.

[0039] Comparative Example 2

[0040] The difference from the embodiment is that no safflower is added, and salvia miltiorrhiza polysaccharide (denoted as SMPS) is finally obtained.

[0041] Comparative Example 3

[0042] The safflower polysaccharides and salvia miltiorrhiza polysaccharides prepared in Comparative Examples 1 and 2 were mixed in a mass ratio of 1:3 to obtain salvia miltiorrhiza and safflower mixed polysaccharides (denoted as SC3:1).

[0043] Characterization:

[0044] The polysaccharides prepared in Example 1 to Comparative Examples 1 to 3 were subjected to infrared spectroscopy detection, and the results obtained are shown in Figure 7-8 .from Figure 7 It can be seen that the composite polysaccharide 3S1C prepared in Example 1 has a peak at 3297.98 cm -1 The broad strong absorption peak at 2939.68 cm is the stretching vibration of -OH. The complex polysaccharide also has intramolecular or intermolecular hydrogen bonds. -1 There is a characteristic peak caused by CH stretching vibration at 1651.86 cm -1 The stretching vibration of the carboxyl C=O is caused by Figure 8 It can be seen that 1500~400cm -1 The composite polysaccharide 3S1C prepared in the embodiment within the fingerprint region contains the functional groups unique to salvia miltiorrhiza polysaccharide and safflower polysaccharide. The composite polysaccharide has a peak at 1530.07 cm -1 There is a NH angle vibration peak of protein. 1408.95 cm -1 The absorption peak is caused by the CH angle vibration; 1234.32 cm -1There is absorption near 1100-1010 cm -1 Between, 1075.69 cm -1 There is only one group of strong absorption peaks at 587.68 cm -1 The presence of a weak peak can preliminarily prove that the complex polysaccharide contains β -type pyranose. Although the composite polysaccharide prepared in Example 1 contains the functional groups of safflower polysaccharide in Comparative Example 1 and salvia miltiorrhiza polysaccharide in Comparative Example 2, the contents of the components contained therein are significantly different. In the composite polysaccharide prepared in Example 1, except for the glucose content which is lower than that of safflower polysaccharide, the contents of other monosaccharide components are higher than those of the polysaccharides prepared in Comparative Examples 1 to 3, among which the differences in arabinose, glucuronic acid and mannose are the most significant.

[0045] By testing the molecular weight of polysaccharides, Fig. 9 As shown, the average relative molecular weight (weight average molecular weight) of salvia miltiorrhiza polysaccharide is 10273 Da, the average relative molecular weight (weight average molecular weight) of safflower polysaccharide is 11799 Da, and the average relative molecular weight (weight average molecular weight) of salvia miltiorrhiza-safflower complex polysaccharide is 11048 Da.

[0046] Test Example 1

[0047] (1) Drought stress resistance test

[0048] Wheat seeds of Jimai 22 were used as experimental materials.

[0049] Take 1600 full and uniform wheat seeds and sterilize them, and divide them into 8 groups, 200 seeds in each group. Configure 20wt% PEG solution (PEG6000 dissolved in water) to simulate drought conditions, add the salvia miltiorrhiza safflower composite polysaccharide prepared in the embodiment, and make the concentration of salvia miltiorrhiza safflower composite polysaccharide in the PEG solution be 10, 20, 50, 100, 200, 500ng / mL, respectively, and record them as 1-10 group, 1-20 group, 1-50 group, 1-100 group, 1-200 group, 1-500 group. Take wheat seeds soaked in 20% PEG solution without adding polysaccharide as CK1 group, and wheat seeds grown under normal environment as CK0 group. Soak 8 groups of wheat seeds for 20 hours under each group treatment (CK0 group is not treated). Take out the seeds of each group and dry them.

[0050] 100 seeds of wheat seeds in each of the above 8 groups were randomly selected and placed in a culture dish with absorbent cotton and cultured at room temperature (25°C). The total number of germinated seeds and the number of newly germinated seeds were recorded at the same time every day until the 7th day. When measuring the indicators, 6 seedlings were randomly selected from each culture dish to measure the root length and sprout length; and the complete embryos were peeled to measure the physiological indicators (germination rate, germination potential, germination index, seedling activity index, germination drought resistance index and vitality drought resistance index) (germination rate = number of seeds germinated within 7 days / total number of seeds; germination potential = number of seeds germinated within 3 days / total number of seeds; germination index = ∑ number of germinations per day / corresponding number of germination days; seedling activity index = germination index × sprout length at 7 days; germination drought resistance index = germination index of each treatment group / germination index of CK0 group; vitality drought resistance index = seedling activity index of each treatment group / seedling activity index of CK0 group). The results are shown in Table 1.

[0051] The remaining 100 wheat seeds in each group were cultivated at room temperature (25°C) for 14 days, and the wheat seedlings were photographed. Figure 1~2 .

[0052] Table 1 Effects of different treatments on wheat germination and seedling growth under drought stress

[0053]

[0054] *The same letters in each column indicate that the difference is not significant (P>0.05), and different letters indicate that the difference is significant (P<0.05).

[0055] Depend on Figure 1~2 It can be seen that the germination rate, germination potential, germination index and activity index of wheat seeds in the drought stress treatment group CK1 showed an overall downward trend compared with the control group CK0. Therefore, PEG6000 at a concentration of 20% can effectively simulate drought stress, and drought stress can inhibit the germination of wheat seeds. The germination potential of wheat seeds treated with different concentrations of Salvia miltiorrhiza-Carthamus tinctorius 3:1 composite polysaccharide solution was improved compared with the CK1 control group, among which the germination rate of wheat seeds treated with 10 ng / mL and 50 ng / mL increased significantly, increasing by 5% and 3% compared with CK1; under drought stress, the germination index and activity index of seeds treated with low concentration (10 ng / mL) and high concentration (200 ng / mL, 500 ng / mL) were higher than those of the control group CK1, and the 10 ng / mL seed soaking treatment had the best effect.

[0056] According to the radicle and plumule data in Table 1, it was found that: compared with CK1, seed soaking treatment with different concentrations of 3:1 compound polysaccharide solution can resist drought stress to varying degrees and promote the growth of wheat seedling roots; seed soaking treatment with 10, 200, and 500 ng / mL compound polysaccharides can promote the growth of wheat germ, while the length of the germ in the 20, 50, and 100 ng / mL concentration treatment groups was lower than that in the CK1 control group, with no obvious effect. Seed soaking treatment with 10 ng / mL 3:1 compound polysaccharide solution had a significant effect on the radicle and plumule of wheat seedlings, increasing by 27.06% and 33.46% respectively compared with CK1.

[0057] (2) Low temperature stress resistance test

[0058] The dwarf tomato variety Micro-Tom (3 leaves and 1 heart stage) was used as the experimental material.

[0059] Take 80 tomato plants with the same growth and divide them into 8 groups, 10 plants in each group. The salvia miltiorrhiza and safflower composite polysaccharide prepared in the example is configured into 10, 20, 50, 100, 200, 500 ng / mL polysaccharide aqueous solutions. During storage, the polysaccharide aqueous solutions are sprayed on the leaves of the tomato plants through foliar spraying, and the spraying treatment is performed once every two days (spraying amount 4 mL / plant). The tomato plants sprayed in each group are recorded as 1-10 group, 1-20 group, 1-50 group, 1-100 group, 1-200 group, and 1-500 group. The tomato plants sprayed with the same amount of water are taken as the CK1 group, and the tomato plants grown in a normal environment (25°C) are taken as the CK0 group. The above 8 groups were all cultured for 7 days, and all groups except the CK0 group were cultured at 4°C at low temperature (low temperature culture was carried out under alternating light for 16 hours and dark for 8 hours). After 7 days of low temperature cultivation, the plants were returned to normal temperature for 2 days, and the growth indicators of tomato plants in each group were tested (plant height, root length, leaf area, chlorophyll, and fresh weight of aboveground / underground parts). The results are shown in Table 2. Four tomato plants with the same growth were selected from each group and photographed. Figure 3~4 .

[0060] Table 2 Effects of different treatments on the growth of tomato seedlings under low temperature stress

[0061]

[0062] *The same letters in each column indicate that the difference is not significant (P>0.05), and different letters indicate that the difference is significant (P<0.05).

[0063] Tomatoes are plants that are highly sensitive to cold. Low temperature stress can easily cause slow growth of tomato plants, yellowing and wilting of leaves, and affect the growth of tomatoes. Figure 3~4It can be seen that different concentrations of Salvia miltiorrhiza-Safflower 3:1 composite polysaccharide spraying treatments have different effects on the growth and development of tomatoes under low temperature stress. In terms of plant height, foliar spraying of 20 ng / mL and 500 ng / mL of Salvia miltiorrhiza and Safflower composite polysaccharides significantly increased by 4.46% and 2.55% compared with CK1, and there were no significant differences in other treatment groups; the results of the treatment groups compared with CK1 showed that the root length of plants sprayed with 20 ng / mL, 100 ng / mL and 200 ng / mL of Salvia miltiorrhiza and Safflower composite polysaccharides increased significantly, and the root fresh weight was measured. Among them, the 20 ng / mL treatment group increased significantly by 26.37% compared with CK1, and the difference was the most obvious. Combined with indicators such as leaf area and chlorophyll, the results showed that foliar spraying of 20 ng / mL of Salvia miltiorrhiza and safflower complex polysaccharides can effectively increase leaf area, increase chlorophyll content, improve photosynthesis, and reduce the impact of chilling injury on tomato growth. The experiment measured that the maximum leaf area of ​​tomatoes under this treatment increased by 23.83% compared with CK1, and the fresh weight of the aboveground part increased by 47.47% compared with CK1.

[0064] Test Example 2

[0065] (1) Drought stress resistance test

[0066] Wheat seeds of Jimai 22 were used as experimental materials.

[0067] 1200 wheat seeds with full quality and uniform size were taken and sterilized, and divided into 6 groups with 200 seeds in each group. A 20wt% PEG solution was prepared, and the polysaccharides prepared in the embodiment and comparative examples 1 to 3 were added respectively, so that the polysaccharide concentration in the PEG solution reached 10ng / mL, which were recorded as the embodiment group and comparative example 1 to 3 groups. Wheat seeds grown under normal conditions were used as the CK0 group; wheat seeds soaked in 20% PEG solution without adding polysaccharides were used as the CK1 group.

[0068] The above 6 groups of wheat seeds were soaked for 20 hours under each treatment group (CK0 group was not treated). The seeds of each group were taken out and dried, and 100 seeds were randomly selected from each group and cultured for 7 days (the specific treatment was the same as the drought stress test in Test Example 1). The germination rate, germination potential, germination index and activity index of the wheat seeds were recorded, and the results are shown in Table 3.

[0069] The remaining 100 wheat seeds in each group were cultivated at room temperature for 14 days and photographed. Figure 5 .

[0070] Table 3 Effects of each treatment group on wheat seeds under drought stress

[0071]

[0072] *The same letters in each column indicate that the difference is not significant (P>0.05), and different letters indicate that the difference is significant (P<0.05).

[0073] As can be seen from Table 1, compared with the CK0 group, the CK1 group shows that drought stress can inhibit the germination of wheat seeds. Compared with the CK1 group and the comparative examples 1 to 3 groups, after the wheat seeds under drought stress were soaked in the embodiment group, the germination rate of wheat seeds was the highest and the seedlings grew best. This shows that the salvia miltiorrhiza-safflower composite polysaccharide obtained by co-extracting salvia miltiorrhiza and safflower at a mass ratio of 3:1 can improve the germination of seeds and the growth ability of seedlings under drought stress.

[0074] according to Figure 5 It can be seen that the growth potential of wheat in the embodiment group is significantly higher than that in the CK1 group and the comparative examples 1 to 3 groups.

[0075] (2) Low temperature stress resistance test

[0076] The dwarf variety Micro-Tom (3 leaves and 1 heart stage) was used as the experimental material.

[0077] 60 tomato plants with uniform growth were taken and divided into 6 groups, with 10 plants in each group. Except for the CK0 group, which was cultured under normal conditions (25°C) for 7 days, the tomato plants in the other groups were cultured at low temperature for 7 days at 4°C (alternating 16 hours of light and 8 hours of darkness). The polysaccharides prepared in the examples and comparative examples 1 to 3 were configured into a 100 ng / mL polysaccharide aqueous solution. During the low-temperature culture period, the above-mentioned polysaccharide aqueous solution was sprayed on the leaves of each group of tomato plants through foliar spraying, and the spraying treatment was once every two days (spray an appropriate amount of 4 mL / plant); spraying an equal amount of clean water was used as the CK1 group (the specific treatment was the same as the low temperature stress resistance test in Test Example 1). After the low-temperature culture was completed, the room temperature was restored for 2 days, and the growth indicators of the tomato plants in each group were tested. The results are shown in Table 4. Four tomato plants with uniform growth were selected from each group for photographing. Figure 6 .

[0078] Table 4 Effects of different treatments on the growth of tomato seedlings under low temperature stress

[0079]

[0080] *The same letters in each column indicate that the difference is not significant (P>0.05), and different letters indicate that the difference is significant (P<0.05).

[0081] From Table 4 and Figure 6It can be seen that compared with the CK0 group, the CK1 group shows that low temperature stress can delay plant growth; compared with the CK1 group and the comparative examples 1 to 3 groups, the growth indexes of tomatoes in the embodiment group are significantly improved. This shows that the salvia miltiorrhiza-safflower composite polysaccharide extracted from salvia miltiorrhiza and safflower at a mass ratio of 3:1 can promote the growth and development of plants under low temperature stress.

[0082] In summary, the present invention confirms that in terms of improving crop stress resistance, seed soaking with Danshen-Carthamus tinctorius composite polysaccharide solution can effectively promote the germination of wheat seeds and the growth of wheat seedlings under drought stress, among which the treatment effect with a concentration of 10 ng / mL is the most significant; the test results on tomato resistance to cold damage show that spraying a certain concentration of composite polysaccharide solution can improve plant photosynthesis and improve the inhibitory effect of cold damage on tomato plant growth, among which the concentration of 20 ng / mL has the best effect. This shows that Danshen-Carthamus tinctorius composite polysaccharide can effectively promote the growth of crops under abiotic stress conditions, and provides a certain theoretical basis and technical support for the application of crop stress resistance.

[0083] The above description is only the preferred embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. An application of a plant-derived Chinese medicine combination extract in regulating plant growth, characterized in that: The plant-derived Chinese medicine combination extract is a salvia miltiorrhiza and safflower polysaccharide complex; The salvia miltiorrhiza and safflower composite polysaccharide is prepared by the following method: The salvia miltiorrhiza and safflower were mixed in a mass ratio of 3:1, crushed and passed through a 40-mesh sieve; 400 g of deionized water was added; the polysaccharide was extracted by ultrasonic assisted extraction, the ultrasonic frequency was 30KHz, the power was 150 W, and the ultrasonic time was 30 min; after extraction, the water extract was obtained by centrifugation and filtration, and sevage reagent was added to the water extract to remove protein, and the mass ratio of the water extract to the sevage reagent was 4:1; ethanol was added to the upper aqueous phase to make the final ethanol concentration about 70wt%, and the mixture was refrigerated and allowed to stand at 4°C, filtered after 24 hours, and freeze-dried at -30°C for 48 hours to obtain the salvia miltiorrhiza and safflower composite polysaccharide; The regulating plant growth includes alleviating crop damage caused by abiotic stress and improving crop stress resistance; The abiotic stress is drought stress, and the stress resistance is the germination of crop seeds and the growth of seedlings under drought stress; the concentration of the Salvia miltiorrhiza-Safflower composite polysaccharide is 10 ng / mL or 200-500 ng / mL; The abiotic stress is low temperature stress, and the stress resistance is the growth and development of crops under low temperature stress; the concentration of the Salvia miltiorrhiza-Safflower complex polysaccharide is 20 ng / mL or 500 ng / mL.

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