An anti-aging liposome composition and its application

Abstract

The present invention relates to an anti-aging liposome composition and its application. The composition comprises 0.1-1 parts of Sophora japonica bud extract, 1-4 parts of Pueraria root extract, 2-6 parts of aminobutyric acid, 50-60 parts of polyol, 2-6 parts of emulsifier, 9-18 parts of auxiliary emulsifier, 0.2-1 parts of cholesterol, 10-35 parts of water, and the auxiliary emulsifier comprises auxiliary emulsifier I polysorbate-20 and auxiliary emulsifier II PPG-6 decyl tetradecanol polyether-30, with a mass ratio of 1-4:8-14; during preparation, the Sophora japonica bud extract, Pueraria root extract, polyol, cholesterol, emulsifier, and auxiliary emulsifier I are first mixed and homogenized, and then water and aminobutyric acid are added; then the auxiliary emulsifier II is added after cooling. The present invention innovatively combines the Sophora japonica bud extract, Pueraria root extract, and aminobutyric acid in a liposome system, solves the solubility and absorption rate problems of the main active ingredients in the Sophora japonica bud extract and Pueraria root extract, and achieves a multi-pathway anti-aging effect.

Description

Technical Field

[0001] The invention belongs to the field of cosmetics, and in particular relates to an anti-aging liposome composition and application thereof. Background Art

[0002] Skin aging factors include endogenous and exogenous factors. Endogenous factors include natural aging such as cell metabolism, hormones, and genetics. Exogenous factors include external environmental sunlight, external stimulation, pollution, temperature, and bad eating, sleeping, and living habits. Photoaging is the main factor among environmental factors. Anti-aging is the most important effect of skin care products. Existing anti-aging products have unclear anti-sun pathways, or single pathways and poor effects.

[0003] Sophora japonica bud extract is rich in quercetin, which has excellent antioxidant, anti-UV and anti-inflammatory effects. During actual application, it was found that the main active ingredient of Sophora japonica flower is "quercetin", which has poor solubility in water and poses difficulties in application.

[0004] Pueraria root extract is rich in puerarin, which has whitening, anti-oxidation, and UVA / UVB absorption effects. It is also commonly used in cosmetics, but its water solubility is average and its absorption efficiency is low.

[0005] Aminobutyric acid, also known as aminobutyric acid, 4-aminobutyric acid, and pipecolic acid, has a molecular formula of C4H9NO2 and a molecular weight of 103. In the field of skin care, it has the functions of improving fine lines and promoting the production of collagen and elastin. However, when used in large amounts, it may cause irritation to the skin. Summary of the invention

[0006] In view of the above problems, the present invention solves the solubility problem of the main active ingredients in Sophora japonica bud extract and Pueraria lobata extract through liposome encapsulation technology, and at the same time, compounding aminobutyric acid with plant-derived ingredients can reduce its irritation, thereby achieving multi-pathway and efficient anti-aging. The obtained anti-aging liposome composition has excellent antioxidant, anti-sugar, anti-inflammatory, and anti-photodamage effects, promotes the production of elastin and collagen, significantly reduces fine lines, and tightens the skin.

[0007] The first aspect of the present invention provides an anti-aging liposome composition, comprising the following components in parts by weight: 0.1-1 parts of Sophora japonica bud extract, 1-4 parts of Pueraria root extract, 2-6 parts of aminobutyric acid, 50-60 parts of polyol, 2-6 parts of emulsifier, 9-18 parts of auxiliary emulsifier, 0.2-1 parts of cholesterol, and 10-35 parts of water; the auxiliary emulsifier includes auxiliary emulsifier I and auxiliary emulsifier II; the auxiliary emulsifier I is polysorbate-20, and the auxiliary emulsifier II is PPG-6 decyltetradecyl alcohol polyether-30; according to the mass ratio, the auxiliary emulsifier I: auxiliary emulsifier II = 1-4: 8-14; the liposome composition is prepared by the following steps:

[0008] S1. The formulated amount of Sophora japonica bud extract, Pueraria root extract, polyol, cholesterol, emulsifier, and co-emulsifier I are mixed and homogenized at 65 to 75 ° C, and then water and aminobutyric acid are added;

[0009] S2. Lower the temperature to 45-55°C, add the co-emulsifier II, and stir for 30-45 minutes to obtain the anti-aging liposome composition.

[0010] Sophora japonica L. extract is a bud extract of the leguminous plant Sophora japonica L., and its main active ingredient is quercetin; Pueraria root extract is a brown powder extract extracted from the dried roots of the leguminous plant Pueraria japonica. In addition to a large amount of starch, the main active ingredient is puerarin. Both ingredients are insoluble in water. First, the effective active ingredients in Sophora japonica L. extract and Pueraria root extract are pre-dissolved at high temperature (65-75 ℃) with polyols, and then emulsifiers are added to encapsulate and stabilize the two ingredients to form a liposome structure, thereby improving the water solubility of Sophora japonica L. extract and Pueraria root extract, improving the absorption efficiency, so that they can be better used in the cosmetic system to play their own efficacy. Similarly, aminobutyric acid is added for emulsification and coating to form a sustained-release function to prevent small molecules of aminobutyric acid from directly penetrating the skin and causing irritation.

[0011] Preferably, the anti-aging liposome composition of the embodiment of the present invention comprises the following components in parts by weight: 0.2-0.8 parts of Sophora japonica bud extract, 1-3 parts of Pueraria root extract, 3-5 parts of aminobutyric acid, 50-60 parts of polyol, 2-5 parts of emulsifier, 12-16 parts of co-emulsifier, 0.2-0.7 parts of cholesterol, and 10-25 parts of water.

[0012] Further preferably, the following components are included in parts by weight: 0.4 parts of Sophora japonica bud extract, 2 parts of Pueraria root extract, 4 parts of aminobutyric acid, 56 parts of polyol, 4 parts of emulsifier, 15 parts of co-emulsifier, 0.4 parts of cholesterol, and 17.6 parts of water.

[0013] Preferably, the polyol consists of glycerol and 1,3-propylene glycol in a weight ratio of 25-30:25-30, more preferably, the weight ratio is 28:28.

[0014] Preferably, the emulsifier is soy lecithin.

[0015] Preferably, the ratio of co-emulsifier I to co-emulsifier II is 3:12.

[0016] Furthermore, the liposome composition of the present invention may also include an antioxidant, a preservative, etc. The antioxidant may be common sodium pyrosulfite, etc.; the preservative may be p-hydroxyacetophenone, etc.

[0017] Preferably, when preparing the liposome composition of the embodiment of the present invention, in the step S1, the mixing speed is 60-120 r / min at 65-75° C., and the homogenization speed is 2800-4200 r / min.

[0018] Another aspect of the present invention also provides the use of the anti-aging liposome composition in anti-aging cosmetics.

[0019] The cosmetics may be one or more of essence water, lotion, essence, gel, facial mask, toner, cream, and sunscreen.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] The present invention solves the solubility and absorption efficiency problems of the main active ingredients of Sophora japonica bud extract and Pueraria lobata extract by encapsulating them in liposomes, and innovatively combines the three components of Sophora japonica bud extract, Pueraria lobata extract and aminobutyric acid in a liposome system, thereby achieving multi-pathway anti-aging effects, having excellent antioxidant, anti-sugar, anti-inflammatory and anti-photodamage effects, promoting the production of elastin and collagen, significantly reducing fine lines and tightening the skin, so that it can be better used in cosmetics. DETAILED DESCRIPTION

[0022] The present invention is further described below with reference to specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0023] Unless otherwise specified, the reagents and materials used in the following examples are commercially available.

[0024] Example 1

[0025] An anti-aging liposome composition. Every 100 g of the composition consists of the following raw materials: 2 g of kudzu root extract, 0.4 g of sophora japonica bud extract, 28 g of glycerol, 28 g of 1,3-propylene glycol, 4 g of soybean lecithin, 3 g of polysorbate-20, 0.4 g of cholesterol, 4 g of aminobutyric acid, 12 g of PPG-6 decyltetradecyl alcohol polyether-30, 0.6 g of p-hydroxyacetophenone, and 17.6 g of water.

[0026] The preparation process is as follows: (1) Pueraria root extract, Sophora japonica bud extract, polyol, soy lecithin, polysorbate-20, and cholesterol are mixed at 70°C and 90 r / min, homogenized at 3500 r / min for 3 min, stirred at 70°C and 90 r / min for 30 min, a preservative is added, stirred at 70°C and 90 r / min for 15 min, water and aminobutyric acid are added, and stirring is continued to cool;

[0027] (2) The temperature was lowered to 50° C., PPG-6 decyltetradecyl alcohol polyether-30 was added, and the mixture was stirred for 45 minutes to obtain the anti-aging liposome composition.

[0028] Example 2

[0029] An anti-aging liposome composition. Every 100 g of the composition consists of the following raw materials: 1 g of kudzu root extract, 0.1 g of sophora japonica bud extract, 30 g of glycerol, 30 g of 1,3-propylene glycol, 2 g of soybean lecithin, 1 g of polysorbate-20, 0.2 g of cholesterol, 3 g of aminobutyric acid, 8 g of PPG-6 decyltetradecyl alcohol polyether-30, 0.3 g of p-hydroxyacetophenone, and 24.4 g of water.

[0030] The preparation process is as follows: (1) Pueraria root extract, Sophora japonica bud extract, polyol, soybean lecithin, polysorbate-20, and cholesterol are mixed at 70°C and 60 r / min, homogenized at 2800 r / min for 3 min, stirred at 65°C and 60 r / min for 30 min, a preservative is added, stirred at 65°C and 60 r / min for 15 min, water and aminobutyric acid are added, and stirring is continued to cool;

[0031] (2) The temperature was lowered to 45° C., PPG-6 decyltetradecyl alcohol polyether-30 was added, and the mixture was stirred for 30 minutes to obtain the anti-aging liposome composition.

[0032] Example 3

[0033] An anti-aging liposome composition. Every 100 g of the composition consists of the following raw materials: 4 g of kudzu root extract, 1 g of sophora japonica bud extract, 25 g of glycerol, 25 g of 1,3-propylene glycol, 6 g of soybean lecithin, 4 g of polysorbate-20, 1 g of cholesterol, 6 g of aminobutyric acid, 14 g of PPG-6 decyltetradecyl alcohol polyether-30, 1 g of p-hydroxyacetophenone, and 13 g of water.

[0034] The preparation process is as follows: (1) mixing kudzu root extract, sophora japonica bud extract, polyol, soy lecithin, polysorbate-20, and cholesterol at 75°C and 120 r / min, homogenizing at 4200 r / min for 3 min, stirring at 75°C and 120 r / min for 30 min, adding preservatives, stirring at 65°C and 4200 r / min for 15 min, adding water and aminobutyric acid, and continuing to stir and cool;

[0035] The temperature was lowered to 55° C., PPG-6 Decyltetradecyl Alcohol Polyether-30 was added, and stirred for 30 minutes to obtain the anti-aging liposome composition.

[0036] Comparative Example 1 is the same as Example 1, except that the Sophora japonica bud extract is replaced by Pueraria root extract.

[0037] Comparative Example 2 is the same as Example 1, except that the Pueraria root extract is removed.

[0038] Comparative Example 3 is the same as Example 1, except that the amount of aminobutyric acid is reduced to 1%.

[0039] Comparative Example 4 is the same as Example 1, except that all glycerol is replaced by 1,3-propylene glycol.

[0040] Comparative Example 5 is the same as Example 1, except that the amounts of polysorbate 20 and PPG-6 decyltetradecyl alcohol polyether-30 are 0.5% and 7%, respectively.

[0041] Comparative Example 6 is the same as Example 1, except that the soybean lecithin and cholesterol in the preparation process are added in process step (2). The specific process is as follows:

[0042] (1) Pueraria root extract, Sophora japonica bud extract, polyol, and polysorbate 20 were mixed at 70°C and 90 r / min, homogenized at 3500 r / min for 3 min, stirred at 70°C and 90 r / min for 30 min, preservatives were added, stirred at 70°C and 90 r / min for 15 min, water and aminobutyric acid were added, and stirring was continued to cool down;

[0043] (2) The temperature was lowered to 50° C., PPG-6 decyltetradecyl alcohol polyether-30, soybean lecithin, and cholesterol were added, and the mixture was stirred for 45 minutes to obtain the liposome mixture.

[0044] Comparative Example 7 is the same as Example 1, except that the homogenization speed in the preparation process is changed to 2000 r / min.

[0045] Comparative Example 8 is the same as Example 1, except that the temperature of the preparation process step (1) is adjusted to 60°C.

[0046] The composition table of each embodiment and comparative example is shown in Table 1.

[0047] Table 1 Composition table of each embodiment and comparative example

[0048]

[0049] Experimental Example 1: Stability Test

[0050] The products of all the examples and comparative examples were placed at 5°C, 25°C, and 45°C under natural light for 60 days to observe whether the products showed any unstable phenomena such as delamination, discoloration, and precipitation. The results are shown in Table 2.

[0051] Table 2 Test results of Experimental Example 1

[0052]

[0053] Experimental Example 2

[0054] 1) Antioxidant activity test

[0055] DPPH is a nitrogen-centered free radical with very stable properties. The ethanol solution of DPPH is purple and has strong absorption at a wavelength of 517nm. When the free radical scavenger works, it will pair with DPPH electrons, causing the light absorption intensity of its ethanol solution to be weakened to varying degrees, and fading to varying degrees. According to the linear relationship between the degree of fading and the number of electrons received by the test sample, the ability of the test sample to scavenge free radicals can be calculated. According to the method of patent ZL202210253643.1 "A composition with soothing effect, its preparation and application", the ability of the products obtained in Examples 1 to 3 and Comparative Examples 1 to 3 to scavenge free radicals was tested, and their antioxidant activity was evaluated.

[0056] 2) Anti-inflammatory test

[0057] Test mechanism: Lipoxygenase catalyzes the reaction of linoleic acid to generate hydroperoxide with a conjugated diene structure, which has a characteristic absorption peak at 234nm. The inhibition rate of lipoxygenase can be calculated by the change in absorbance value.

[0058] Specific method: Set up sample wells (T) in a 96-well microplate and add 10uL / well of the test sample solution, and use an equal volume of solvent as a blank well (C). Add 40uL / well of lipoxygenase solution, shake for 1min, and incubate at 37℃ for 5min. Add 160μL of linoleic acid substrate to react, shake the plate for 1min, and incubate at 25℃ for 5min. Scan the wavelength and measure the absorbance at 0 point and after 5min at 237nm on the microplate reader. And calculate the lipoxygenase activity inhibition rate according to "lipoxygenase enzyme activity inhibition rate (%) = (△C-△T) / △C×100%".

[0059] The anti-inflammatory effects of the products obtained from Examples 1 to 3 and Comparative Examples 1 to 3 were tested.

[0060] 3) Anti-glycation test

[0061] The non-enzymatic protein degradation process changes the structure and function of proteins and other substances. The glycosylated proteins in the human body are called advanced glycation end products (AGEs). The continuous accumulation of AGEs will solidify collagen fibers, reduce skin elasticity, and make the skin yellow and dark, showing an aged appearance. It is known that most AGEs are fluorescent. By comparing the fluorescence values ​​of AGEs in the experimental group and the control group, the inhibition rate of active substances on AGEs can be calculated, thereby evaluating the anti-glycation effect of cosmetics.

[0062] Specific test method: Use 5ml centrifuge tubes to set up sample tubes (T), sample blank tubes (T0), negative control tubes (C), and negative control blank tubes (C0). Among them, the sample tube is a reaction tube for sample plus protein and sugar, the sample blank tube (T0) is a reaction tube for sample plus protein, the negative control tube is a reaction tube for buffer solution plus protein and sugar, and the negative control blank tube is a reaction tube for buffer solution plus protein. For each sample tube (T) of each tested concentration, 3 parallel tubes need to be set up, and 3 parallel tubes for negative control tubes (C) also need to be set up.

[0063] Add 1 ml sample solution, 0.5 ml bovine serum albumin solution, and 0.5 ml glucose solution to the sample tube (T); add 1 ml sample solution, 0.5 ml bovine serum albumin solution, and 0.5 ml PBS buffer to the sample blank tube (T0); add 1 ml PBS buffer solution, 0.5 ml bovine serum albumin solution, and 0.5 ml glucose solution to the negative control group; add 1 ml PBS buffer solution, 0.5 ml bovine serum albumin solution, and 0.5 ml PBS buffer solution to the negative control blank group; vortex for 1 min to mix well, filter (0.22 μm PES or nylon filter membrane) and store in a 5 ml centrifuge tube.

[0064] Place the above-mentioned sample tubes in a constant temperature and humidity incubator and incubate at 60°C for 40 hours (equivalent to incubation at 30°C for 60 days). Take out each sample after 40 hours, draw 200 μL on a black ELISA plate and detect it with an ELISA reader. The detection settings are: no plate cover-detection-fluorescence intensity-excitation 360 / 40nm, emission 460 / 40nm, top, gain 50, detection height 1mm, test, and record the fluorescence absorption value. The anti-glycation effect is calculated according to "glycation end product clearance rate % = (1-(T-T0) / (C-C0)) × 100%".

[0065] The anti-glycation effects of the products obtained in Examples 1 to 3 and Comparative Examples 1 to 3 were tested.

[0066] 4) Anti-allergic soothing activity test

[0067] Hyaluronidase is a participant in allergic reactions. It decomposes hyaluronic acid in the body and turns it into a low molecular weight acidic irritant, causing the release of histamine and inducing sensitive symptoms in the body. The hyaluronidase inhibition test is a typical in vitro method for evaluating anti-allergic activity. The hyaluronidase inhibition rate is used as an indicator to evaluate the anti-allergic activity of the sample. The higher the inhibition rate, the stronger the anti-allergic activity of the sample. That is, the lower the IC50 value of the sample's hyaluronidase inhibition rate, the stronger the anti-allergic activity of the sample. According to the hyaluronidase in vitro inhibition method in Patent ZL202210253643.1 "A composition with soothing effect, its preparation and application", the anti-allergic and soothing effects of the products obtained in Examples 1 to 3 and Comparative Examples 1 to 3 were tested.

[0068] 5) Test to promote collagen production

[0069] The collagen content enhancement test was conducted according to the method "T / SHRH 031-2020 Cosmetic firming and anti-wrinkle efficacy test-in vitro fibroblast type I collagen content determination". The test sample concentration was 1%. The test results are shown in Table 3.

[0070] Table 3 Test results of experimental example 2

[0071]

[0072] The experimental results show that after combination, the anti-aging effect of the product is significantly improved.

[0073] Experimental Example 3 Transdermal Absorption Experiment

[0074] The products of all Example 1 and Comparative Examples 4-6, as well as a 2% dispersion of Pueraria extract and a 0.4% dispersion of Sophora japonica bud extract were used as samples, wherein the dispersion solvent was 1,3-propylene glycol, and the transdermal absorption capacity was determined by the following method:

[0075] ① Use a hair puller to remove the fine hair on the pig skin (complete, undamaged, fresh), rinse with clean water, and then use a sharp knife to remove the subcutaneous fat. Wash with PBS solution. After processing, wrap it with tin foil, place it flat in a box, and store it at -20℃ for later use.

[0076] ② Before the experiment, the pig skin was taken out 30 min in advance and thawed in PBS solution. The transdermal experiment was carried out in the Franz diffusion cell device. First, the skin was clamped between the supply chamber and the receiving chamber. The temperature of the receiving chamber was 32 °C, the magnetic stirring speed was 600 r / min, the sample addition amount was 500 μL, and the effective permeation area of ​​the skin was 0.98 cm 2 The receiving solution was a PBS (pH = 7.4) solution containing 5% Tween 80 by mass, with a volume of 15 mL.

[0077] ③ After the reaction is completed for 24 hours, carefully remove the device, take out the skin sandwiched between the two chambers, rinse with clean water, wipe with ethanol to remove the sample on the surface, and finally use filter paper to absorb the water on the surface for standby use.

[0078] ④ Determination of the content of Pueraria root extract and Sophora japonica bud extract in the epidermis: The tape stripping method was used for determination. Specifically, the skin was spread flat on a glass dish, and 21 layers of skin were removed with tape (3M, Scotch 600). In order to avoid residual samples on the skin surface, the first layer was discarded, and the remaining 20 layers were combined and placed in a 10 mL centrifuge tube. 2 mL of anhydrous ethanol was added, vortexed for 3 min, and centrifuged at 2000 r / min for 5 min. The clear liquid was taken and passed through a 220 nm organic membrane. The content of Pueraria root extract and Sophora japonica bud extract (calibrated with puerarin and quercetin, the same below) was determined by HPLC (μg / cm 2 ).

[0079] Determination of the content of Pueraria extract in the dermis: Cut the skin with 21 layers removed with scissors, put it into a 10 mL centrifuge tube, add 1 mL of anhydrous ethanol, vortex for 3 min, centrifuge at 2000 r / min for 5 min, take the upper clear liquid, repeat twice, combine the supernatants, pass through a 220 nm organic membrane, and determine the content of Pueraria extract and Sophora japonica bud extract in the dermis by HPLC (μg / cm 2 ), the results are shown in Table 4. The absorption amount was calculated by this formula, Qs=V×C, (Qs: the absorption amount of the active substance in each layer of the skin; V: the total volume of the extract; C: the concentration of the active substance in the extract.) The content of Pueraria extract in the skin = the content of Pueraria extract in the dermis + the content of Pueraria extract in the epidermis, and according to the data in Table 4 and the formula "increase percentage %=[Pueraria extract content-control group (2% Pueraria extract dispersion)] / control group (2% Pueraria extract dispersion)×100%", the increase percentage of Pueraria extract in the epidermis, dermis and skin was further calculated. The calculation method of Sophora japonica bud extract is the same, and the results are shown in Table 5.

[0080] Table 4 Test results of Pueraria extract content in skin

[0081]

[0082] Table 5 Results of the test on the changes of content in the skin

[0083]

[0084] It can be seen that the transdermal absorption effects of the Sophora japonica bud extract and the Pueraria root extract of the anti-aging liposomes obtained in Example 1 are significantly better than those of Comparative Examples 4 to 6 and the control group, indicating that the anti-aging liposomes prepared by the method of the present invention greatly improve the transdermal absorption capacity of the active ingredients.

[0085] Experimental Example 4 Essence containing anti-aging liposomes

[0086] (1) Preparation of serum containing anti-aging liposomes

[0087] S1. 88.25wt% water, 0.1wt% sodium hyaluronate, 1.5wt% betaine, and 0.35wt% ammonium acryloyldimethyltaurate / VP copolymer were mixed, and the mixture was heated to 80°C at 300 r / min while stirring to obtain phase A.

[0088] S2. 3 wt% butanediol, 0.3 wt% p-hydroxyacetophenone, 0.5 wt% hexanediol, and 1.0 wt% pentanediol were mixed, heated to 70 °C, and dispersed uniformly to obtain phase B;

[0089] S3. Cool phase A to 45° C., add phase B, stir evenly, then add 5.0 wt % of the product obtained in Example 1, stir evenly, and obtain the essence.

[0090] (2) Human testing method

[0091] First, the volunteers were screened by dermatologists. They were required to have non-sensitive skin, skin that was not in a sensitive state and had no skin diseases, no bacterial, fungal or viral infections on the facial skin, and no use of any anti-aging cosmetics, medicines, health products and medical beauty treatments within three months before the start of the trial. Volunteers with other facial features that might affect the test measurement results were excluded. Finally, 30 volunteers (18 females and 12 males, aged 35-55 years old) were screened out and the pre-use data were measured: ① The facial images of the volunteers were taken with VISIA in visible light mode, and the scores of the volunteers' wrinkles around their eyes were obtained by analysis (reflecting the size, area and density of wrinkles, the smaller the score, the fewer wrinkles); ② The Multi Skin Test Center manufactured by Courag+Khazaka (German CK) was used. Cutometer elastic probe in MPA580 measures skin elasticity and skin firmness around the eyes (skin elasticity is based on parameter R2, which is the ratio of rebound amount Ua without negative pressure to maximum pull-up amount Uf with negative pressure (i.e. R2=Ua / Uf). The larger the value, the better the skin elasticity; skin firmness is based on parameter F4, which is obtained after 10 cycles of measurement by Cutometer probe. The smaller the value, the firmer the skin); ③ Professional dermatologists refer to the outer corner wrinkle scoring The wrinkles at the outer corners of the volunteers' eyes were scored according to the standard (scoring standard for wrinkles at the outer corners of the eyes: 0 points for level 0 wrinkles, no wrinkles; 1 point for level 1 wrinkles, slightly visible and unnoticeable shallow wrinkles; 2 points for level 2 wrinkles, slightly visible and obvious shallow wrinkles; 3 points for level 3 wrinkles, visible and obvious shallow wrinkles; 4 points for level 4 wrinkles, slightly visible and obvious shallow wrinkles with slightly deeper wrinkles; 5 points for level 5 wrinkles, visible slightly deeper wrinkles; 6 points for level 6 wrinkles, visible and obvious deep wrinkles; 7 points for level 7 wrinkles, visible and very obvious deep wrinkles).

[0092] After that, a 28-day smear test was started. During the 28-day test period, volunteers were given (2.0±0.1) mg / cm 2 Apply cosmetic 1 to the face (once in the morning and once in the evening after cleaning the face with water), and do not apply other cosmetics or skin care products during this period. On the 14th and 28th days, sit in an environment with a temperature of 21±1 ℃ and a humidity of 50%±10% for more than 30 minutes, then use VISIA to take images of the volunteers' eye periorbital areas in visible light mode again, analyze the volunteers' eye periorbital wrinkle scores, use the Cutometer elasticity probe to measure the volunteers' eye periorbital skin elasticity R2 and skin firmness F4, and have dermatologists score the volunteers' wrinkles at the outer corners of the eyes. Volunteers are not allowed to use any cosmetics or skin care products (including cosmetic 1 used for testing) on ​​the day of the test.

[0093] (3) Human test results

[0094] According to "change rate (%) = (after use - before use) / before use × 100%", the change rate of periocular wrinkle score, skin elasticity R2 change rate, skin firmness F4 change rate and outer canthus wrinkle score change rate were calculated respectively (the results are shown in Table 6).

[0095] Table 6 Human test results of serum

[0096]

[0097] It can be seen that the essence product containing the anti-aging liposome composition of Example 1 can significantly improve the volunteers' skin conditions such as wrinkles around the eyes, skin elasticity and firmness, and all volunteers did not experience adverse reactions during use, indicating that the cosmetics of the present invention have high safety and excellent anti-aging effects.

[0098] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0099] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. An anti-aging liposome composition, characterized in that: The invention comprises the following components in parts by weight: 0.1-1 parts of Sophora japonica bud extract, 1-4 parts of Pueraria root extract, 2-6 parts of aminobutyric acid, 50-60 parts of polyol, 2-6 parts of emulsifier, 9-18 parts of auxiliary emulsifier, 0.2-1 parts of cholesterol, 10-35 parts of water, The polyol is composed of glycerol and 1,3-propylene glycol in a weight ratio of 25-30:25-30; The emulsifier is soybean lecithin; The auxiliary emulsifier includes auxiliary emulsifier I and auxiliary emulsifier II; the auxiliary emulsifier I is polysorbate-20, and the auxiliary emulsifier II is PPG-6 decyl tetradecyl alcohol polyether-30; according to the mass ratio, the auxiliary emulsifier I: auxiliary emulsifier II = 1-4: 8-14; The liposome composition is prepared by the following steps: S1. The formula amount of Sophora japonica bud extract, Pueraria extract, polyol, cholesterol, emulsifier, co-emulsifier I was mixed at 65-75 ° C at 60-120r / min, 2800-4200r / min homogenization, and then water and aminobutyric acid were added; S2. Lower the temperature to 45-55°C, add the co-emulsifier II, and stir for 30-45 minutes to obtain the anti-aging liposome composition.

2. The anti-aging liposome composition according to claim 1, characterized in that: The invention comprises the following components in parts by weight: 0.2-0.8 parts of Sophora japonica bud extract, 1-3 parts of Pueraria root extract, 3-5 parts of aminobutyric acid, 50-60 parts of polyol, 2-5 parts of emulsifier, 12-16 parts of auxiliary emulsifier, 0.2-0.7 parts of cholesterol and 10-25 parts of water.

3. The anti-aging liposome composition according to claim 2, characterized in that: The invention comprises the following components in parts by weight: 0.4 parts of Sophora japonica bud extract, 2 parts of Pueraria root extract, 4 parts of aminobutyric acid, 56 parts of polyol, 4 parts of emulsifier, 15 parts of auxiliary emulsifier, 0.4 parts of cholesterol and 17.6 parts of water.

4. The anti-aging liposome composition according to claim 3, characterized in that: The weight ratio of the glycerol to 1,3-propylene glycol is 28:

28.

5. The anti-aging liposome composition according to claim 1, characterized in that: In terms of mass ratio, co-emulsifier I: co-emulsifier II = 3:

12.

6. Use of the anti-aging liposome composition according to any one of claims 1 to 5 in anti-aging cosmetics.

7. The use according to claim 6, characterized in that: The cosmetics are one or more of lotion, essence, gel, facial mask, toner and cream.

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