A device for extracting bovine serum albumin

Abstract

The invention relates to the technical field of serum protein extraction, and discloses a bovine serum protein extraction device, comprising: an extraction cylinder; a cylinder cover; a rotary centrifugal mechanism, the rotary centrifugal mechanism is installed on the extraction cylinder, one end of the rotary centrifugal mechanism is arranged in the inner cavity of the extraction cylinder, and the other end is arranged at the bottom of the extraction cylinder; the rotary centrifugal mechanism comprises a motor, a transmission shaft is fixedly installed on the output shaft of the motor, the transmission shaft is rotatably installed in a supporting sleeve, a rotating base is fixedly installed on the top end of the transmission shaft, a centrifugal cylinder is detachably installed on the rotating base, and an annular ultrafiltration membrane is plugged and installed on the inner cavity side wall of the centrifugal cylinder; wherein, a plurality of separation micropores are opened on the side wall of the centrifugal cylinder; and a plurality of groups of serum protein extraction mechanisms. The device requires less equipment to be used when extracting protein from bovine blood, and the operation procedure and steps are relatively simple, and the serum protein in bovine blood can be simply and effectively stripped and extracted.

Description

Technical Field

[0001] The invention relates to the technical field of serum protein extraction, in particular to a device for extracting bovine serum protein. Background Art

[0002] In the prior art, bovine serum albumin is extracted using a bovine serum albumin extraction device. When using the bovine serum albumin extraction device to extract serum albumin, the plasma and blood cells in the bovine blood need to be separated. After the plasma is separated, the serum and protein in the plasma need to be stripped and extracted.

[0003] However, a Chinese utility model disclosed in the publication number CN221028210U discloses a device for extracting bovine serum albumin, comprising:

[0004] A centrifugal cylinder, wherein a protective box is fixedly installed on the top of the centrifugal cylinder;

[0005] A moving frame, the outer wall of the protection box is slidably connected with the moving frame;

[0006] A connecting rod is fixedly installed on the outer wall of the movable frame, a convex frame is arranged on the top of the centrifugal cylinder, bolts are installed through the outer wall of the movable frame, a hollow annular frame is fixedly connected to the bottom end of the connecting rod, a nozzle is installed through the bottom of the hollow annular frame, a sealing frame is fixedly installed on the bottom of the hollow annular frame, a water inlet is connected through the top of the convex frame, a motor 1 is fixedly installed on the top of the centrifugal cylinder, a rotating rod is fixedly connected to the output end of the motor 1, and a scraper is fixedly connected to the outer wall of the rotating rod.

[0007] When the device is used to extract bovine serum albumin, a large number of devices are needed, and the procedures and steps are relatively complicated, and the serum albumin in the bovine blood cannot be extracted simply and effectively.

[0008] Based on this, a device for extracting bovine serum albumin is now proposed. Summary of the invention

[0009] 1. Technical issues to be resolved

[0010] In view of the shortcomings of the prior art, the present invention provides a bovine serum albumin extraction device to solve the problem raised in the background technology: the bovine serum albumin extraction device in the prior art requires the use of more equipment when extracting bovine serum albumin, and the procedures and steps are relatively complicated, and it is impossible to simply and effectively extract serum albumin from bovine blood.

[0011] (II) Technical solution

[0012] To achieve the above object, the present invention provides the following technical solutions:

[0013] A device for extracting bovine serum albumin, comprising:

[0014] An extraction cylinder, wherein an assembly hole is provided at the center of the bottom circle of the extraction cylinder, a support sleeve is fixedly installed at the center of the bottom circle of the inner cavity of the extraction cylinder, a plurality of discharge holes are provided at the bottom edge of the extraction cylinder, and a plurality of discharge pipes are fixedly installed at the bottom edge of the extraction cylinder;

[0015] The inner cavities of the support sleeve and the assembly hole are interconnected, a plurality of discharge holes are circumferentially distributed at the bottom of the extraction cylinder, the position of each discharge pipe corresponds to the corresponding discharge hole one by one, and the inner cavities are interconnected;

[0016] A cylinder cover, which is detachably mounted on the top opening of the extraction cylinder, a feed hole is provided at the center of the top circle of the cylinder cover, and a circular inner groove is provided at the bottom of the cylinder cover;

[0017] A rotary centrifugal mechanism, wherein the rotary centrifugal mechanism is mounted on the extraction cylinder, one end of the rotary centrifugal mechanism is arranged in the inner cavity of the extraction cylinder, and the other end of the rotary centrifugal mechanism is arranged at the bottom of the extraction cylinder;

[0018] The rotary centrifugal mechanism comprises a motor, the motor is fixedly mounted at the bottom center of the extraction cylinder, and the output shaft of the motor is upwardly penetrated into the supporting sleeve through the assembly hole, a transmission shaft is fixedly mounted on the output shaft of the motor, the transmission shaft is rotatably mounted in the supporting sleeve, one end of the top of the transmission shaft is flush with the top of the supporting sleeve, one end of the top of the transmission shaft is fixedly mounted with a rotating base, a centrifugal cylinder is detachably mounted on the rotating base, the top of the centrifugal cylinder is engaged in the circular inner groove, and an annular ultrafiltration membrane is plugged and mounted on the inner cavity side wall of the centrifugal cylinder;

[0019] Wherein, a plurality of separation micropores are provided on the side wall of the centrifugal cylinder; and

[0020] A plurality of groups of serum protein extraction mechanisms are provided, and each group of the serum protein extraction mechanisms can be detachably mounted on a corresponding discharge pipe.

[0021] Preferably, each group of separation micropores is distributed in a circular pattern on the side wall of the centrifugal cylinder.

[0022] Preferably, the outer wall of the annular ultrafiltration membrane and the inner wall of the centrifugal cylinder are in contact with each other;

[0023] The thickness of the annular ultrafiltration membrane is 10 mm to 30 mm.

[0024] Preferably, a plurality of positioning holes are provided on the top edge of the rotating base, and a plurality of positioning posts are fixedly installed on the bottom edge of the centrifugal cylinder, and the position of each positioning post corresponds to the corresponding positioning hole one by one;

[0025] When the centrifugal cylinder is installed on the rotating base, each of the positioning posts can be inserted and installed in the corresponding positioning hole.

[0026] Preferably, an annular notch is provided on the bottom edge of the cylinder cover, and the cylinder cover is mounted on the top opening of the extraction cylinder through the annular notch;

[0027] A sealing plug is inserted and installed in the feed hole.

[0028] Preferably, each group of the serum protein extraction mechanism comprises an upper cylindrical shell and a lower cylindrical shell, and each of the lower cylindrical shells can be detachably mounted on the corresponding upper cylindrical shell;

[0029] Each of the upper cylindrical shells can be detachably installed in a corresponding discharge pipe;

[0030] A discharge pipe is integrally formed at the bottom of each lower cylindrical shell;

[0031] A stripping component is installed in the inner cavity of each lower cylindrical shell.

[0032] Preferably, a discharge valve is fixedly installed on each of the discharge pipes.

[0033] Preferably, a feed pipe is integrally formed at the top center of each upper cylindrical shell, and each feed pipe is plugged and installed in the inner cavity of the corresponding discharge pipe;

[0034] The bottom of each upper cylindrical shell is integrally formed with an annular flange, and a slot is formed between each annular flange and the bottom edge of the upper cylindrical shell;

[0035] The top opening of the lower cylindrical shell is sleeved and installed on the annular flange, and after the sleeve connection, one end of the top of the lower cylindrical shell is abutted against the bottom of the upper cylindrical shell.

[0036] Preferably, each group of the stripping components comprises an upper fixed clamp ring and a lower fixed clamp ring fixedly mounted in the inner cavity of the lower cylindrical shell, the upper fixed clamp ring and the lower fixed clamp ring are staggered up and down, and a circular ultrafiltration membrane is fixedly mounted between the upper fixed clamp ring and the lower fixed clamp ring;

[0037] Wherein, the thickness of the circular ultrafiltration membrane is 10mm-30mm;

[0038] The upper fixing clamp ring and the lower fixing clamp ring can clamp and fix the circular ultrafiltration membrane.

[0039] Preferably, the lower cylindrical shell is interference fit with the annular flange.

[0040] Beneficial effects:

[0041] The present invention provides a bovine serum albumin extraction device, which has the following beneficial effects:

[0042] 1. In the present invention, bovine blood is added to the inner cavity of the centrifuge cylinder through the feed hole. After the bovine blood is added, the rotary centrifuge mechanism is operated. Specifically, the motor is started. The motor can drive the rotation of the transmission shaft through the high-speed rotation of its output shaft. The rotation of the transmission shaft can drive the rotation of the rotating base. The rotating base can drive the rotation of the centrifuge cylinder when rotating. Therefore, the output shaft of the motor can make the centrifuge cylinder rotate at a high speed in the inner cavity of the extraction cylinder while rotating at a high speed. When the centrifuge cylinder rotates at a high speed, the bovine blood in the centrifuge cylinder can separate the plasma and blood cells in the bovine blood under the action of the rotating centrifugal force. The plasma can penetrate the annular ultrafiltration membrane and the separation micropores under the action of the rotating centrifugal force and enter the inner cavity of the extraction cylinder, while the blood cells can be filtered by the annular ultrafiltration membrane and remain on the annular ultrafiltration membrane.

[0043] Under the action of the annular ultrafiltration membrane, the blood cells in the bovine blood were also extracted;

[0044] The plasma entering the inner cavity of the extraction cylinder can enter the discharge pipe through multiple discharge holes, and then enter the serum protein extraction mechanism through the discharge pipe. In the serum protein extraction mechanism, the protein in the plasma can be stripped and extracted, and the serum in the plasma can be precipitated outwards.

[0045] Therefore, under the action of the serum protein extraction mechanism, the protein in the bovine blood can be effectively extracted.

[0046] Second, in the present invention, the device requires less equipment to be used when extracting protein from bovine blood, and the operation procedures and steps are relatively simple, and the serum protein in bovine blood can be stripped and extracted simply and effectively.

[0047] 3. In the present invention, after the work is completed, since the cylinder cover is detachably mounted on the extraction cylinder, and the centrifugal cylinder is detachably mounted on the rotating base, the annular ultrafiltration membrane can be taken out and replaced after the cylinder cover is removed from the extraction cylinder.

[0048] Fourth, in the present invention, after the centrifuge cylinder is disassembled from the rotating base, the centrifuge cylinder can be conveniently cleaned.

[0049] 5. In the present invention, since each group of serum protein extraction mechanisms can be disassembled from the corresponding discharge pipe, after each group of serum protein extraction mechanisms completes the extraction of protein from bovine blood, each group of serum protein extraction mechanisms is disassembled one by one to facilitate sampling of the protein extracted from the bovine blood therein.

[0050] 6. In the present invention, a stripping component is installed in the inner cavity of each lower cylindrical shell. After the plasma enters the serum protein extraction mechanism through the discharge pipe, the serum and protein in the plasma can be stripped through the stripping component inside it, and the serum can be precipitated through the stripping component. The precipitated serum can be discharged outward through the discharge pipe. At the same time, the stripping component can filter the protein, and the filtered protein can remain on the stripping component, thereby effectively completing the extraction of protein from bovine blood.

[0051] 7. In the present invention, since the upper cylindrical shell and the discharge pipe can be disassembled, and the upper cylindrical shell and the lower cylindrical shell can be disassembled, after the protein extraction is completed, the upper cylindrical shell and the lower cylindrical shell are disassembled from the discharge pipe, and then the upper cylindrical shell and the lower cylindrical shell are disassembled. After the two are disassembled, the stripping component in the lower cylindrical shell can be exposed to the outside, and at this time, it is convenient for the staff to extract the protein on the stripping component.

[0052] 8. In the present invention, a feed tube is integrally formed at the top center of each upper cylindrical shell, and each feed tube is plugged and installed in the inner cavity of the corresponding discharge tube. Since the feed tube is plugged and installed in the discharge tube, the serum protein extraction mechanism can be easily disassembled from the discharge tube.

[0053] 9. In the present invention, the bottom of each upper cylindrical shell is integrally formed with an annular flange, and a slot is formed between each annular flange and the bottom edge of the upper cylindrical shell;

[0054] The top opening of the lower cylindrical shell is sleeved and installed on the annular flange, and after the sleeve connection, one end of the top of the lower cylindrical shell abuts against the bottom of the upper cylindrical shell, so that the lower cylindrical shell and the upper cylindrical shell can be easily installed and disassembled.

[0055] 10. In the present invention, each set of stripping components includes an upper fixed clamp ring and a lower fixed clamp ring fixedly installed in the inner cavity of the lower cylindrical shell, the upper fixed clamp ring and the lower fixed clamp ring are staggered up and down, and a circular ultrafiltration membrane is fixedly installed between the upper fixed clamp ring and the lower fixed clamp ring;

[0056] The upper fixing clamp and the lower fixing clamp can clamp and fix the circular ultrafiltration membrane. The upper fixing clamp and the lower fixing clamp can effectively clamp and fix the circular ultrafiltration membrane. After the circular ultrafiltration membrane is clamped and fixed, the serum and protein in the plasma can be stripped through the circular ultrafiltration membrane. The serum can penetrate the circular ultrafiltration membrane downward, and the protein can be filtered by the circular ultrafiltration membrane. The filtered protein can remain on the circular ultrafiltration membrane, thereby effectively realizing the stripping of serum and protein. After stripping, the extraction of protein in bovine blood is effectively completed.

[0057] 11. In the present invention, when the centrifuge cylinder is installed on the rotating base, each positioning post can be inserted and installed in the corresponding positioning hole. The centrifuge cylinder can be inserted and matched with the multiple positioning holes opened on the rotating base through the multiple positioning posts at the bottom of the centrifuge cylinder to achieve rapid locking, so that the centrifuge cylinder can be quickly installed from the rotating base and can also be quickly removed from the rotating base.

[0058] 12. In the present invention, an annular notch is provided on the bottom edge of the cylinder cover, and the cylinder cover is mounted on the top opening of the extraction cylinder through the annular notch. The cylinder cover can be mounted on the top of the extraction cylinder through the annular notch on the bottom edge of the cylinder, so that the cylinder cover can be easily installed or removed from the extraction cylinder. BRIEF DESCRIPTION OF THE DRAWINGS

[0059] Figure 1 A three-dimensional schematic diagram of a partial cross section of the present invention;

[0060] Figure 2 A three-dimensional schematic diagram of a partial cross-section of the present invention viewed from above;

[0061] Figure 3 It is a three-dimensional schematic diagram of the present invention;

[0062] Figure 4 It is a three-dimensional schematic diagram of the combination of the extraction cylinder and the cylinder cover of the present invention;

[0063] Figure 5 It is a three-dimensional schematic diagram of the extraction cylinder and the cylinder cover of the present invention when they are combined and viewed from above;

[0064] Figure 6 It is a three-dimensional schematic diagram of the combination of the motor, the transmission shaft and the rotating base of the present invention;

[0065] Figure 7 It is a three-dimensional schematic diagram of the combination of the centrifugal cylinder and the annular ultrafiltration membrane of the present invention;

[0066] Figure 8 A three-dimensional schematic diagram of a centrifugal cylinder of the present invention viewed from above;

[0067] Fig. 9 is a three-dimensional schematic diagram of a partial cross section of the serum protein extraction mechanism of the present invention;

[0068] Fig.10 It is a schematic explosion diagram of a partial section of the serum protein extraction mechanism of the present invention.

[0069] In the figure: 10, extraction cylinder; 1001, assembly hole; 1002, support sleeve; 1003, discharge hole; 1004, discharge pipe; 20, cylinder cover; 2001, feed hole; 2002, circular inner groove; 30, rotary centrifugal mechanism; 3001, motor; 3002, transmission shaft; 3003, rotating base; 30031, positioning hole; 3004, centrifugal cylinder; 30041, positioning Column; 30042, separation micropore; 3005, annular ultrafiltration membrane; 40, serum protein extraction mechanism; 4001, upper cylindrical shell; 40011, feed pipe; 40012, annular flange; 4002, lower cylindrical shell; 40021, discharge pipe; 4003, discharge valve; 4004, upper fixing clamp; 4005, lower fixing clamp; 4006, circular ultrafiltration membrane; 50, sealing plug. DETAILED DESCRIPTION

[0070] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0071] Embodiment 1

[0072] like Figure 1-8 As shown, the present invention provides a technical solution:

[0073] A device for extracting bovine serum albumin, comprising:

[0074] An extraction cylinder 10, wherein an assembly hole 1001 is provided at the center of the bottom circle of the extraction cylinder 10, a support sleeve 1002 is fixedly installed at the center of the bottom circle of the inner cavity of the extraction cylinder 10, a plurality of discharge holes 1003 are provided at the bottom edge of the extraction cylinder 10, and a plurality of discharge pipes 1004 are fixedly installed at the bottom edge of the extraction cylinder 10;

[0075] The inner cavities of the support sleeve 1002 and the assembly hole 1001 are interconnected, and a plurality of discharge holes 1003 are distributed circumferentially at the bottom of the extraction cylinder 10. The position of each discharge pipe 1004 corresponds to the corresponding discharge hole 1003 one by one, and the inner cavities are interconnected;

[0076] The cylinder cover 20 is detachably mounted on the top opening of the extraction cylinder 10. A feed hole 2001 is provided at the center of the top of the cylinder cover 20, and a circular inner groove 2002 is provided at the bottom of the cylinder cover 20;

[0077] A rotary centrifugal mechanism 30, wherein the rotary centrifugal mechanism 30 is mounted on the extraction cylinder 10, and one end of the rotary centrifugal mechanism 30 is disposed in the inner cavity of the extraction cylinder 10, and the other end of the rotary centrifugal mechanism 30 is disposed at the bottom of the extraction cylinder 10;

[0078] The rotary centrifugal mechanism 30 includes a motor 3001, which is fixedly mounted at the bottom center of the extraction cylinder 10, and the output shaft of the motor 3001 is inserted upward from the assembly hole 1001 into the support sleeve 1002, a transmission shaft 3002 is fixedly mounted on the output shaft of the motor 3001, and the transmission shaft 3002 is rotatably mounted in the support sleeve 1002, and one end of the top of the transmission shaft 3002 is flush with the top of the support sleeve 1002, and a rotating base 3003 is fixedly mounted on one end of the top of the transmission shaft 3002, and a centrifugal cylinder 3004 is detachably mounted on the rotating base 3003, and the top of the centrifugal cylinder 3004 is engaged in the circular inner groove 2002, and an annular ultrafiltration membrane 3005 is plugged and installed on the inner cavity side wall of the centrifugal cylinder 3004;

[0079] Among them, a plurality of separation micropores 30042 are opened on the side wall of the centrifugal cylinder 3004;

[0080] When the centrifugal cylinder 3004 rotates, the top outer wall of the centrifugal cylinder 3004 can slide and cooperate with the inner side wall of the circular inner groove 2002; and

[0081] There are multiple groups of serum protein extraction mechanisms 40 , and each group of serum protein extraction mechanisms 40 can be detachably installed on the corresponding discharge pipe 1004 .

[0082] In this embodiment, bovine blood is added to the inner cavity of the centrifuge cylinder 3004 through the feed hole 2001. After the bovine blood is added, the rotary centrifuge mechanism 30 is put into operation. Specifically, the motor 3001 is started. The motor 3001 can drive the transmission shaft 3002 to rotate through the high-speed rotation of its output shaft. The rotation of the transmission shaft 3002 can drive the rotation of the rotating base 3003. The rotating base 3003 can also drive the rotation of the centrifuge cylinder 3004 when rotating. Therefore, the output shaft of the motor 3001 rotates at a high speed. At the same time, the centrifuge cylinder 3004 can be rotated at a high speed in the inner cavity of the extraction cylinder 10. When the centrifuge cylinder 3004 is rotated at a high speed, the bovine blood in the centrifuge cylinder 3004 can be separated from the plasma and blood cells in the bovine blood under the action of the rotating centrifugal force. The plasma can penetrate the annular ultrafiltration membrane 3005 and the separation micropores 30042 under the action of the rotating centrifugal force and enter the inner cavity of the extraction cylinder 10, while the blood cells can be filtered by the annular ultrafiltration membrane 3005 and remain on the annular ultrafiltration membrane 3005.

[0083] Under the action of the annular ultrafiltration membrane 3005, the blood cells in the bovine blood are also extracted.

[0084] The plasma entering the inner cavity of the extraction cylinder 10 can enter the discharge pipe 1004 through multiple discharge holes 1003, and then enter the serum protein extraction mechanism 40 through the discharge pipe 1004. In the serum protein extraction mechanism 40, the protein in the plasma can be stripped and extracted, and the serum in the plasma can be precipitated outward.

[0085] Therefore, under the action of the serum protein extraction mechanism 40, the protein in the bovine blood can be effectively extracted.

[0086] The device requires fewer devices when extracting proteins from bovine blood, and the operating procedures and steps are relatively simple, and the serum proteins in bovine blood can be stripped and extracted simply and effectively.

[0087] Furthermore, in this embodiment, after the work is completed, since the cylinder cover 20 is detachably mounted on the extraction cylinder 10, and the centrifugal cylinder 3004 is detachably mounted on the rotating base 3003, after the cylinder cover 20 is removed from the extraction cylinder 10, the annular ultrafiltration membrane 3005 can be taken out and replaced;

[0088] At the same time, after the centrifugal cylinder 3004 is disassembled from the rotating base 3003, the centrifugal cylinder 3004 can be easily cleaned.

[0089] Since each group of serum protein extraction mechanisms 40 can be disassembled from the corresponding discharge pipe 1004, after each group of serum protein extraction mechanisms 40 completes the extraction of protein from the bovine blood, each group of serum protein extraction mechanisms 40 is disassembled one by one to facilitate sampling of the protein extracted from the bovine blood therein.

[0090] Embodiment 2

[0091] like Figure 1 , Figure 6 , Figure 7 as well as Figure 8 As shown, based on the first embodiment, the following improvements are made:

[0092] Furthermore, each group of separation micropores 30042 is distributed in a circular manner on the side wall of the centrifuge tube 3004, and each separation micropore 30042 can allow plasma to penetrate outward.

[0093] Furthermore, the outer wall of the annular ultrafiltration membrane 3005 and the inner wall of the centrifugal cylinder 3004 are in contact with each other;

[0094] The thickness of the annular ultrafiltration membrane 3005 is 10 mm to 30 mm, and the annular ultrafiltration membrane 3005 can separate blood cells and plasma in bovine blood.

[0095] Furthermore, a plurality of positioning holes 30031 are provided on the top edge of the rotating base 3003, and a plurality of positioning posts 30041 are fixedly installed on the bottom edge of the centrifugal cylinder 3004, and the position of each positioning post 30041 corresponds to the corresponding positioning hole 30031 one by one;

[0096] When the centrifuge barrel 3004 is installed on the rotating base 3003, each positioning column 30041 can be plugged into the corresponding positioning hole 30031. The centrifuge barrel 3004 can be quickly locked by plugging and cooperating with the multiple positioning holes 30031 opened on the rotating base 3003 through the multiple positioning columns 30041 at the bottom of the centrifuge barrel 3004, so that the centrifuge barrel 3004 can be quickly installed from the rotating base 3003, and can also be quickly removed from the rotating base 3003.

[0097] Embodiment 3

[0098] like Figure 1-4 As shown, based on the first embodiment, the following improvements are made:

[0099] Furthermore, an annular notch is provided at the bottom edge of the cylinder cover 20, and the cylinder cover 20 is mounted on the top opening of the extraction cylinder 10 through the annular notch. The cylinder cover 20 can be mounted on the top of the extraction cylinder 10 through the annular notch at the bottom edge thereof, so that the cylinder cover 20 can be easily installed or removed from the extraction cylinder 10;

[0100] A sealing plug 50 is inserted and installed in the feed hole 2001. After the sealing plug 50 is inserted and installed in the feed hole 2001, it can form a blocking effect to prevent the high-speed rotating cow blood in the centrifuge cylinder 3004 from splashing outward.

[0101] Embodiment 4

[0102] like Figure 1-3 as well as Figure 9-10 As shown, based on the first embodiment, the following improvements are made:

[0103] Further, each set of serum protein extraction mechanism 40 includes an upper cylindrical shell 4001 and a lower cylindrical shell 4002, and each lower cylindrical shell 4002 can be detachably mounted on the corresponding upper cylindrical shell 4001;

[0104] Each upper cylindrical shell 4001 can be detachably installed in the corresponding discharge pipe 1004;

[0105] A discharge pipe 40021 is integrally formed at the bottom of each lower cylindrical shell 4002;

[0106] A stripping component is installed in the inner cavity of each lower cylindrical shell 4002. After the plasma enters the serum protein extraction mechanism 40 through the discharge pipe 1004, the serum and protein in the plasma can be stripped through the stripping component inside it, and the serum can be precipitated through the stripping component. The precipitated serum can be discharged outward through the discharge pipe 40021. At the same time, the stripping component can filter the protein, and the filtered protein can remain on the stripping component, thereby effectively completing the extraction of protein from bovine blood.

[0107] Since the upper cylindrical shell 4001 and the discharge pipe 1004 are disassembled, and the upper cylindrical shell 4001 and the lower cylindrical shell 4002 are disassembled, after the protein extraction is completed, the upper cylindrical shell 4001 and the lower cylindrical shell 4002 are removed from the discharge pipe 1004, and then the upper cylindrical shell 4001 and the lower cylindrical shell 4002 are disassembled. After the disassembly of the two is completed, the stripping component in the lower cylindrical shell 4002 can be exposed to the outside, and at this time, it is convenient for the staff to extract the protein on the stripping component.

[0108] Furthermore, a discharge valve 4003 is fixedly installed on each discharge pipe 40021, and the discharge valve 4003 can be used to control the opening and closing of the discharge pipe 40021.

[0109] Furthermore, a feed pipe 40011 is integrally formed at the center of the top of each upper cylindrical shell 4001, and each feed pipe 40011 is plugged and installed in the inner cavity of the corresponding discharge pipe 1004. Since the feed pipe 40011 is plugged and installed in the discharge pipe 1004, the serum protein extraction mechanism 40 can be easily disassembled from the discharge pipe 1004.

[0110] The bottom of each upper cylindrical shell 4001 is integrally formed with an annular flange 40012, and a slot is formed between each annular flange 40012 and the bottom edge of the upper cylindrical shell 4001;

[0111] The top opening of the lower cylindrical shell 4002 is installed on the annular flange 40012, and after the installation, the top end of the lower cylindrical shell 4002 abuts against the bottom of the upper cylindrical shell 4001, so that the lower cylindrical shell 4002 and the upper cylindrical shell 4001 can be easily installed and disassembled.

[0112] Specifically, the lower cylindrical shell 4002 and the annular flange 40012 are interference fit.

[0113] Furthermore, each set of stripping components includes an upper fixed snap ring 4004 and a lower fixed snap ring 4005 fixedly installed in the inner cavity of the lower cylindrical shell 4002, the upper fixed snap ring 4004 and the lower fixed snap ring 4005 are staggered up and down, and a circular ultrafiltration membrane 4006 is fixedly installed between the upper fixed snap ring 4004 and the lower fixed snap ring 4005;

[0114] Among them, the thickness of the circular ultrafiltration membrane 4006 is 10mm-30mm;

[0115] The upper fixing clamp 4004 and the lower fixing clamp 4005 can clamp and fix the circular ultrafiltration membrane 4006. The upper fixing clamp 4004 and the lower fixing clamp 4005 can effectively clamp and fix the circular ultrafiltration membrane 4006. After the circular ultrafiltration membrane 4006 is clamped and fixed, the serum and protein in the plasma can be stripped through the circular ultrafiltration membrane 4006. The serum can penetrate the circular ultrafiltration membrane 4006 downward, and the protein can be filtered by the circular ultrafiltration membrane 4006. The filtered protein can remain on the circular ultrafiltration membrane 4006, thereby effectively realizing the stripping of serum and protein. After stripping, the extraction of protein in bovine blood is effectively completed.

[0116] In summary, the workflow of the present invention is:

[0117] like Figure 1-10 As shown, the bovine blood is added to the inner cavity of the centrifuge cylinder 3004 through the feed hole 2001. After the bovine blood is added, the rotary centrifuge mechanism 30 is put into operation. Specifically, the motor 3001 is started. The motor 3001 can drive the transmission shaft 3002 to rotate through the high-speed rotation of its output shaft. The rotation of the transmission shaft 3002 can drive the rotation of the rotating base 3003. The rotating base 3003 can also drive the rotation of the centrifuge cylinder 3004 when rotating. Therefore, the output shaft of the motor 3001 rotates at a high speed while When the centrifugal cylinder 3004 is rotated at a high speed in the inner cavity of the extraction cylinder 10, the bovine blood in the centrifugal cylinder 3004 can be separated into plasma and blood cells under the action of the rotating centrifugal force. The plasma can penetrate the annular ultrafiltration membrane 3005 and the separation micropores 30042 under the action of the rotating centrifugal force and enter the inner cavity of the extraction cylinder 10, while the blood cells can be filtered by the annular ultrafiltration membrane 3005 and remain on the annular ultrafiltration membrane 3005.

[0118] Under the action of the annular ultrafiltration membrane 3005, the blood cells in the bovine blood are also extracted;

[0119] The plasma entering the inner cavity of the extraction cylinder 10 can enter the discharge pipe 1004 through multiple discharge holes 1003, and then enter the serum protein extraction mechanism 40 through the discharge pipe 1004. In the serum protein extraction mechanism 40, the protein in the plasma can be stripped and extracted, and the serum in the plasma can be precipitated outward.

[0120] Therefore, under the action of the serum protein extraction mechanism 40, the protein in the bovine blood can be effectively extracted.

[0121] The device requires fewer devices when extracting proteins from bovine blood, and the operating procedures and steps are relatively simple, and the serum proteins in bovine blood can be stripped and extracted simply and effectively.

[0122] Furthermore, after the work is completed, since the cylinder cover 20 is detachably mounted on the extraction cylinder 10, and the centrifugal cylinder 3004 is detachably mounted on the rotating base 3003, after the cylinder cover 20 is removed from the extraction cylinder 10, the annular ultrafiltration membrane 3005 can be taken out and replaced;

[0123] At the same time, after the centrifugal cylinder 3004 is disassembled from the rotating base 3003, the centrifugal cylinder 3004 can be easily cleaned.

[0124] Since each group of serum protein extraction mechanisms 40 can be disassembled from the corresponding discharge pipe 1004, after each group of serum protein extraction mechanisms 40 has finished extracting the protein in the bovine blood, each group of serum protein extraction mechanisms 40 is disassembled one by one, so as to facilitate sampling of the protein in the bovine blood extracted therein;

[0125] Each set of serum protein extraction mechanism 40 includes an upper cylindrical shell 4001 and a lower cylindrical shell 4002, and each lower cylindrical shell 4002 can be detachably mounted on the corresponding upper cylindrical shell 4001;

[0126] Each upper cylindrical shell 4001 can be detachably installed in the corresponding discharge pipe 1004;

[0127] A discharge pipe 40021 is integrally formed at the bottom of each lower cylindrical shell 4002;

[0128] A stripping component is installed in the inner cavity of each lower cylindrical shell 4002. After the plasma enters the serum protein extraction mechanism 40 through the discharge pipe 1004, the serum and protein in the plasma can be stripped through the stripping component inside it, and the serum can be precipitated through the stripping component. The precipitated serum can be discharged outward through the discharge pipe 40021. At the same time, the stripping component can filter the protein, and the filtered protein can remain on the stripping component, thereby effectively completing the extraction of protein from bovine blood.

[0129] Since the upper cylindrical shell 4001 and the discharge pipe 1004 can be disassembled, and the upper cylindrical shell 4001 and the lower cylindrical shell 4002 can be disassembled, after the protein extraction is completed, the upper cylindrical shell 4001 and the lower cylindrical shell 4002 are disassembled from the discharge pipe 1004, and then the upper cylindrical shell 4001 and the lower cylindrical shell 4002 are disassembled. After the disassembly of the two is completed, the stripping component in the lower cylindrical shell 4002 can be exposed to the outside, and at this time, it is convenient for the staff to extract the protein on the stripping component;

[0130] A discharge valve 4003 is fixedly installed on each discharge pipe 40021, and the discharge valve 4003 can be used to control the opening and closing of the discharge pipe 40021;

[0131] A feed pipe 40011 is integrally formed at the center of the top of each upper cylindrical shell 4001, and each feed pipe 40011 is plugged and installed in the inner cavity of the corresponding discharge pipe 1004. Since the feed pipe 40011 is plugged and installed in the discharge pipe 1004, the serum protein extraction mechanism 40 can be easily disassembled from the discharge pipe 1004;

[0132] The bottom of each upper cylindrical shell 4001 is integrally formed with an annular flange 40012, and a slot is formed between each annular flange 40012 and the bottom edge of the upper cylindrical shell 4001;

[0133] The top opening of the lower cylindrical shell 4002 is sleeved and installed on the annular flange 40012, and after the sleeve connection, one end of the top of the lower cylindrical shell 4002 abuts against the bottom of the upper cylindrical shell 4001, so that the lower cylindrical shell 4002 and the upper cylindrical shell 4001 can be easily installed and disassembled;

[0134] Each set of stripping components includes an upper fixed snap ring 4004 and a lower fixed snap ring 4005 fixedly installed in the inner cavity of the lower cylindrical shell 4002, the upper fixed snap ring 4004 and the lower fixed snap ring 4005 are staggered up and down, and a circular ultrafiltration membrane 4006 is fixedly installed between the upper fixed snap ring 4004 and the lower fixed snap ring 4005;

[0135] The upper fixing clamp 4004 and the lower fixing clamp 4005 can clamp and fix the circular ultrafiltration membrane 4006. The upper fixing clamp 4004 and the lower fixing clamp 4005 can effectively clamp and fix the circular ultrafiltration membrane 4006. After the circular ultrafiltration membrane 4006 is clamped and fixed, the serum and protein in the plasma can be stripped through the circular ultrafiltration membrane 4006. The serum can penetrate the circular ultrafiltration membrane 4006 downward, and the protein can be filtered by the circular ultrafiltration membrane 4006. The filtered protein can remain on the circular ultrafiltration membrane 4006, thereby effectively realizing the stripping of serum and protein. After stripping, the extraction of protein in bovine blood is effectively completed.

[0136] The above different embodiments can be combined, replaced and used in conjunction with each other.

[0137] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0138] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A bovine serum albumin extraction device, characterized in that: include: An extraction cylinder (10), wherein an assembly hole (1001) is provided at the center of the bottom circle of the extraction cylinder (10), a support sleeve (1002) is fixedly installed at the center of the bottom circle of the inner cavity of the extraction cylinder (10), a plurality of discharge holes (1003) are provided at the bottom edge of the extraction cylinder (10), and a plurality of discharge pipes (1004) are fixedly installed at the bottom edge of the extraction cylinder (10); The inner cavities of the support sleeve (1002) and the assembly hole (1001) are interconnected, a plurality of discharge holes (1003) are distributed in a circumferential pattern at the bottom of the extraction cylinder (10), the position of each discharge pipe (1004) corresponds to the corresponding discharge hole (1003) one by one, and the inner cavities are interconnected; A cylinder cover (20), the cylinder cover (20) being detachably mounted at the top opening of the extraction cylinder (10), a feed hole (2001) being provided at the center of the top circle of the cylinder cover (20), and a circular inner groove (2002) being provided at the bottom of the cylinder cover (20); A rotary centrifugal mechanism (30), the rotary centrifugal mechanism (30) being mounted on the extraction cylinder (10), one end of the rotary centrifugal mechanism (30) being arranged in the inner cavity of the extraction cylinder (10), and the other end of the rotary centrifugal mechanism (30) being arranged at the bottom of the extraction cylinder (10); The rotary centrifugal mechanism (30) comprises a motor (3001), the motor (3001) being fixedly mounted at the bottom center of the extraction cylinder (10), and the output shaft of the motor (3001) being inserted upwards through the assembly hole (1001) into the support sleeve (1002), a transmission shaft (3002) being fixedly mounted on the output shaft of the motor (3001), the transmission shaft (3002) being rotatably mounted in the support sleeve (1002), and the transmission shaft (3002) being rotatably mounted in the support sleeve (1002). One end of the top of the driving shaft (3002) is flush with the top of the supporting sleeve (1002); a rotating base (3003) is fixedly mounted on one end of the top of the driving shaft (3002); a centrifugal cylinder (3004) is detachably mounted on the rotating base (3003); the top of the centrifugal cylinder (3004) is engaged in the circular inner groove (2002); and an annular ultrafiltration membrane (3005) is plugged and mounted on the inner cavity side wall of the centrifugal cylinder (3004); Wherein, a plurality of separation micropores (30042) are provided on the side wall of the centrifugal cylinder (3004); and A plurality of groups of serum protein extraction mechanisms (40), each group of the serum protein extraction mechanisms (40) can be detachably mounted on a corresponding discharge pipe (1004).

2. The device for extracting bovine serum albumin according to claim 1, characterized in that: Each group of separation micropores (30042) is distributed in a circular pattern on the side wall of the centrifugal cylinder (3004).

3. The device for extracting bovine serum albumin according to claim 2, characterized in that: The outer wall of the annular ultrafiltration membrane (3005) and the inner wall of the centrifugal cylinder (3004) are in contact with each other; The thickness of the annular ultrafiltration membrane (3005) is 10 mm to 30 mm.

4. The device for extracting bovine serum albumin according to claim 1, characterized in that: A plurality of positioning holes (30031) are provided on the top edge of the rotating base (3003), and a plurality of positioning posts (30041) are fixedly mounted on the bottom edge of the centrifugal cylinder (3004), wherein the position of each positioning post (30041) corresponds one by one to the corresponding positioning hole (30031).

5. The device for extracting bovine serum albumin according to claim 1, characterized in that: An annular notch is provided on the bottom edge of the cylinder cover (20), and the cylinder cover (20) is mounted on the top opening of the extraction cylinder (10) by snapping into the annular notch; A sealing plug (50) is inserted and installed in the feed hole (2001).

6. The device for extracting bovine serum albumin according to claim 1, characterized in that: Each group of the serum protein extraction mechanism (40) comprises an upper cylindrical shell (4001) and a lower cylindrical shell (4002), and each lower cylindrical shell (4002) can be detachably mounted on the corresponding upper cylindrical shell (4001); Each of the upper cylindrical shells (4001) can be detachably installed in a corresponding discharge pipe (1004); A discharge pipe (40021) is integrally formed at the bottom of each lower cylindrical shell (4002); A stripping component is installed in the inner cavity of each lower cylindrical shell (4002).

7. The device for extracting bovine serum albumin according to claim 6, characterized in that: A discharge valve (4003) is fixedly installed on each of the discharge pipes (40021).

8. The device for extracting bovine serum albumin according to claim 6, characterized in that: A feed pipe (40011) is integrally formed at the center of the top of each upper cylindrical shell (4001), and each feed pipe (40011) is plugged and installed in the inner cavity of the corresponding discharge pipe (1004); The bottom of each upper cylindrical shell (4001) is integrally formed with an annular flange (40012), and a slot is formed between each annular flange (40012) and the bottom edge of the upper cylindrical shell (4001); The top opening of the lower cylindrical shell (4002) is sleeved and installed on the annular flange (40012), and after the sleeved installation, one end of the top of the lower cylindrical shell (4002) is in contact with the bottom of the upper cylindrical shell (4001).

9. The device for extracting bovine serum albumin according to claim 6, characterized in that: Each group of the stripping components comprises an upper fixed clamping ring (4004) and a lower fixed clamping ring (4005) fixedly mounted in the inner cavity of the lower cylindrical shell (4002), the upper fixed clamping ring (4004) and the lower fixed clamping ring (4005) being staggered up and down, and a circular ultrafiltration membrane (4006) being fixedly mounted between the upper fixed clamping ring (4004) and the lower fixed clamping ring (4005); Wherein, the thickness of the circular ultrafiltration membrane (4006) is 10mm-30mm; The upper fixing clamp ring (4004) and the lower fixing clamp ring (4005) are capable of clamping and fixing the circular ultrafiltration membrane (4006).

10. The device for extracting bovine serum albumin according to claim 8, characterized in that: The lower cylindrical shell (4002) and the annular flange (40012) are interference fit.

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