A composite probiotic for improving the flavor of Chinese mitten crab meat and its application

Abstract

The present invention relates to a composite probiotic for improving the flavor of Chinese mitten crab meat and an application thereof. The composite probiotic for improving the flavor of Chinese mitten crab meat comprises Bacillus coagulans Bacillus coagulans BC66 strain, Lactobacillus plantarum Lactobacillus plantarum LP194 strain and Lactobacillus acidophilus Lactobacillus acidophilus LA71 strain. The present invention finds that the above three strains can cooperate with each other and promote each other, and play a synergistic effect in promoting the growth and development of Chinese mitten crab and improving the meat flavor of Chinese mitten crab. When the amount of bacteria used is the same, the three bacteria compound is compared with the combination of any two strains, and the effect of promoting the growth and development of Chinese mitten crab and improving the meat flavor of Chinese mitten crab is significantly improved.

Description

Technical Field

[0001] The invention belongs to the technical field of probiotics and relates to a composite probiotic for improving the flavor of Chinese mitten crab meat and an application thereof. Background Art

[0002] Chinese mitten crab ( Eriocheir sinensis ), also known as Chinese hairy crab and river crab, is an extremely important aquatic economic animal in my country. With the continuous growth of people's consumption demand for Chinese mitten crab, the artificial breeding technology of Chinese mitten crab has developed rapidly, and its breeding output is increasing year by year.

[0003] Generally speaking, the muscle tissue of high-quality Chinese mitten crab is rich in flavor substances such as glutamic acid, alanine and glycine. How to increase the content of flavor substances and improve the quality of Chinese mitten crab has become one of the key factors in promoting the sustainable and efficient development of the Chinese mitten crab breeding industry.

[0004] There are a large number of different types of microorganisms in aquaculture water, on the surface of animals and in their intestines, among which intestinal bacteria have been proven to play an important role in digestion and absorption, immune system and metabolism. Probiotics are relatively popular active microorganisms in recent years. They maintain host health through multiple mechanisms such as regulating the balance of intestinal flora, inhibiting the excessive proliferation of harmful bacteria, promoting the digestion and absorption of nutrients, enhancing the body's immune function, and maintaining the integrity of the mucosal barrier.

[0005] At present, there are experiments that prove that probiotics can effectively improve the quality of broiler meat, but there are still few reports on probiotics improving the flavor of Chinese mitten crab meat. Therefore, the development of a probiotic that can improve the flavor of Chinese mitten crab meat has broad market prospects. Summary of the invention

[0006] In view of the deficiencies of the prior art, the object of the present invention is to provide a composite probiotic for improving the flavor of Chinese mitten crab meat and its application.

[0007] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides a composite probiotic for improving the meat flavor of Chinese mitten crab, wherein the composite probiotic for improving the meat flavor of Chinese mitten crab comprises Bacillus coagulans BC66 strain with a preservation number of CGMCC 21879, Lactobacillus plantarum LP194 strain with a preservation number of CGMCC 21766, and Lactobacillus acidophilus LA71 strain with a preservation number of CGMCC 21765.

[0009] The present invention creatively develops a new probiotic compounding method and a new strategy for improving the meat flavor of Chinese mitten crab, that is, Bacillus coagulans BC66 strain, Lactobacillus plantarum LP194 strain and Lactobacillus acidophilus LA71 strain are compounded and used together. It is found that the three can cooperate with each other and promote each other, and play a synergistic effect in promoting the growth and development of Chinese mitten crab and improving the meat flavor of Chinese mitten crab, which is specifically embodied in: (1) increasing the weight of Chinese mitten crab; (2) increasing the total protein content in the serum of Chinese mitten crab and improving liver function; (3) improving the antioxidant capacity of Chinese mitten crab; (4) increasing the free amino acid content in the body of Chinese mitten crab; (5) improving the sensory quality of Chinese mitten crab; (6) increasing the per mu yield and recapture rate of Chinese mitten crab. When the amount of bacteria used is the same, the combination of BC66, LP194 and LA71 strains has significantly improved the effect of promoting the growth and development of Chinese mitten crab and improving the meat flavor of Chinese mitten crab compared to the intervention method of selecting two of the strains. Therefore, the composite probiotics have a good prospect for preparing Chinese mitten crab feed additives or Chinese mitten crab feed. At the same time, the above three bacteria are all probiotics, the product is highly safe, and it is not easy to produce resistance.

[0010] Preferably, the ratio of the number of viable bacteria of the BC66 strain, LP194 strain and LA71 strain is (1-10):(1-10):(1-10), wherein (1-10) can be selected as 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, etc., and other specific point values ​​within this numerical range can be selected, which will not be described here one by one.

[0011] In a second aspect, the present invention provides a feed additive for the cultivation of Chinese mitten crab, wherein the feed additive contains the composite probiotics described in the first aspect.

[0012] Preferably, the total bacterial count in the feed additive is not less than 1×10 9 CFU / mL or 1×10 9 CFU / g, for example 1×10 9 CFU / g (CFU / mL), 1×10 10 CFU / g (CFU / mL), 2×10 10 CFU / g (CFU / mL), 5×10 10 CFU / g (CFU / mL), 8×10 10 CFU / g (CFU / mL), 1×10 11 CFU / g (CFU / mL), 5×10 11CFU / g (CFU / mL), 1×10 12 CFU / g (CFU / mL), 1×10 13 CFU / g (CFU / mL), 1×10 14 CFU / g (CFU / mL), etc. Other specific point values ​​within this numerical range can be selected and will not be described here one by one.

[0013] Preferably, the dosage form of the feed additive includes lyophilized powder, tablets, granules or solutions.

[0014] Preferably, the dosage form of the feed additive is a freeze-dried powder, which is prepared by a preparation method comprising the following steps:

[0015] The BC66 strain, the LP194 strain and the LA71 strain are inoculated into a culture medium respectively, activated and fermented in sequence to obtain a fermentation broth; the fermentation broth is centrifuged respectively, mixed with a freeze-drying protective agent and freeze-dried to obtain BC66 bacterial powder, LP194 bacterial powder and LA71 bacterial powder; the BC66 bacterial powder, LP194 bacterial powder and LA71 bacterial powder are mixed according to a ratio to obtain the feed additive.

[0016] Preferably, the lyoprotectant is selected from any one or a combination of at least two of skim milk, gelatin, dextrin, gum arabic, dextran, sodium alginate, polyvinyl pyrrolidone, sucrose, lactose, trehalose, sorbitol or xylitol.

[0017] In a third aspect, the present invention provides use of the composite probiotics described in the first aspect or the feed additive described in the second aspect in preparing Chinese mitten crab feed.

[0018] In a fourth aspect, the present invention provides a Chinese mitten crab feed, wherein the Chinese mitten crab feed comprises a basic feed and the feed additive described in the first aspect.

[0019] In a fifth aspect, the present invention provides use of the composite probiotics described in the first aspect or the feed additive described in the second aspect in the preparation of a product for improving the meat flavor of Chinese mitten crab.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] The present invention creatively develops a new probiotic compounding method and a new strategy for improving the meat flavor of Chinese mitten crab, that is, Bacillus coagulans BC66 strain, Lactobacillus plantarum LP194 strain and Lactobacillus acidophilus LA71 strain are compounded and used together. It is found that the three can cooperate with each other and promote each other, and play a synergistic effect in promoting the growth and development of Chinese mitten crab and improving the meat flavor of Chinese mitten crab, which is specifically embodied in: (1) increasing the weight of Chinese mitten crab; (2) increasing the total protein content in the serum of Chinese mitten crab and improving liver function; (3) improving the antioxidant capacity of Chinese mitten crab; (4) increasing the free amino acid content in the body of Chinese mitten crab; (5) improving the sensory quality of Chinese mitten crab; (6) increasing the per mu yield and recapture rate of Chinese mitten crab. When the amount of bacteria used is the same, the combination of BC66, LP194 and LA71 strains has significantly improved the effect of promoting the growth and development of Chinese mitten crab and improving the meat flavor of Chinese mitten crab compared to the intervention method of selecting two of the strains. Therefore, the composite probiotics have a good prospect for preparing Chinese mitten crab feed additives or Chinese mitten crab feed. At the same time, the above three bacteria are all probiotics, the product is highly safe, and it is not easy to produce resistance.

[0022] The BC66 strain involved in the present invention is classified and named Bacillus coagulans, the preservation unit is the General Microbiology Center of China Microorganism Culture Collection Administration, the preservation number is CGMCC 21879, the preservation date is March 8, 2021, and the preservation address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0023] The LP194 strain involved in the present invention is classified and named Lactobacillus plantarum, the preservation unit is the General Microbiology Center of China Microorganism Culture Collection Administration, the preservation number is CGMCC 21766, the preservation date is January 29, 2021, and the preservation address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0024] The LA71 strain involved in the present invention is classified and named Lactobacillus acidophilus, the preservation unit is the General Microbiology Center of China Microorganism Culture Collection Administration, the preservation number is CGMCC 21765, the preservation date is January 29, 2021, and the preservation address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a statistical chart of the recapture rate of river crabs in each group;

[0026] Figure 2 This is a statistical chart of the per-acre yield of each group of river crabs. DETAILED DESCRIPTION

[0027] The BC66 strain involved in the following examples is classified and named Bacillus coagulans, deposited on March 8, 2021, and has a deposit number of CGMCC 21879.

[0028] The LP194 strain involved in the following examples is classified and named Lactobacillus plantarum, with a preservation date of January 29, 2021 and a preservation number of CGMCC 21766.

[0029] The LA71 strain involved in the following examples is classified and named Lactobacillus acidophilus, the preservation time is January 29, 2021, and the preservation number is CGMCC 21765.

[0030] The culture medium involved in the following examples is as follows:

[0031] MRS medium (g / L): peptone 10g / L, beef extract 10g / L, glucose 15g / L, lactose 15g / L, yeast powder 5g / L, diammonium hydrogen citrate 2g / L, K2PO4·3H2O 2.6g / L, MgSO4·7H2O 0.1g / L, MnSO4 0.05g / L, Tween 80 1mL / L, cysteine ​​salt 0.5g / L.

[0032] The compound feed involved in the following examples was purchased from Gangxin (Shanghai) Feed Co., Ltd., model 8261 (38 protein).

[0033] Preparation Example

[0034] Provided are a BC66 bacterial powder product, a LP194 bacterial powder product and a LA71 bacterial powder product, and the preparation methods thereof are as follows:

[0035] (1) Inoculate 3% of the Bacillus coagulans BC66 strain, Lactobacillus plantarum LP194 strain, and Lactobacillus acidophilus LA71 strain stored in -80°C glycerol tubes into 5 mL sterile MRS medium, culture at 37°C for 12 h, and subculture twice to obtain activated strains; then, inoculate 5% of the activated strains into 50 mL sterile MRS medium, culture at 37°C for 12 h, and obtain expanded culture fluid;

[0036] (2) The expanded culture solution was inoculated into 1000 mL of sterilized MRS medium at a 3% inoculum volume, cultured at 37°C, and the concentration of the culture solution was determined. The fermentation time was adjusted according to the concentration of the culture solution to obtain three fermentation solutions with the same concentration of the culture solution;

[0037] (3) The obtained fermented bacterial broth was centrifuged at 8000 r / min for 20 min at 4°C, the supernatant was discarded under sterile conditions, and the bacterial sludge was collected; the collected bacterial sludge was mixed with a 20% lactose aqueous solution at a volume ratio of 1:2 using a vortex machine to obtain a mixture of Bacillus coagulans BC66 and lactose, a mixture of Lactobacillus plantarum LP194 and lactose, and a mixture of Lactobacillus acidophilus LA71 and lactose, respectively;

[0038] (4) The mixture was pre-frozen in a -80°C refrigerator for 3 h to obtain a pre-frozen live bacterial preparation; then, the pre-frozen live bacterial preparation was freeze-dried in a vacuum freeze dryer at a freeze-drying temperature of -50°C and a vacuum degree of 0.20 mBar for 48 h until it was completely dry, and the number of live bacteria was 1×10 9 CFU / g of Bacillus coagulans BC66 powder, Lactobacillus plantarum LP194 powder and Lactobacillus acidophilus LA71 powder were stored in a -4°C refrigerator for later use.

[0039] Example 1

[0040] The present embodiment provides a feed additive, which is composed of Bacillus coagulans BC66 powder, Lactobacillus plantarum LP194 powder and Lactobacillus acidophilus LA71 powder with a live bacteria count ratio of 1:1:1.

[0041] Example 2

[0042] The present embodiment provides a feed additive, which is composed of Bacillus coagulans BC66 powder, Lactobacillus plantarum LP194 powder and Lactobacillus acidophilus LA71 powder with a live bacteria count ratio of 5:4:3.

[0043] Example 3

[0044] The present embodiment provides a feed additive, which is composed of Bacillus coagulans BC66 powder, Lactobacillus plantarum LP194 powder and Lactobacillus acidophilus LA71 powder with a live bacteria count ratio of 2:6:8.

[0045] Comparative Example 1

[0046] This comparative example provides a feed additive, which is composed of commercially available Bacillus coagulans ATCC7050 powder, Lactobacillus plantarum LP194 powder and Lactobacillus acidophilus LA71 powder with a live bacteria count ratio of 1:1:1.

[0047] Comparative Example 2

[0048] This comparative example provides a feed additive, which is composed of Bacillus coagulans BC66 powder and Lactobacillus plantarum LP194 powder with a live bacteria count ratio of 1:1.

[0049] Comparative Example 3

[0050] This comparative example provides a feed additive, which is composed of Bacillus coagulans BC66 powder and Lactobacillus acidophilus LA71 powder with a live bacteria count ratio of 1:1.

[0051] Comparative Example 4

[0052] This comparative example provides a feed additive, which is composed of Lactobacillus plantarum LP194 powder and Lactobacillus acidophilus LA71 powder with a live bacteria count ratio of 1:1.

[0053] Test Case

[0054] (1) The crab seedlings were purchased from the same batch of hatching, and healthy crabs with uniform size and complete appendages were selected for breeding experiments in outdoor ponds (length × width = 23m × 73m, water depth 0.5-1m). They were evenly divided into control group and experimental group S1-S7, with 3 replicates in each group, for a total of 24 ponds. In each pond, yellow silk grass was planted with a row spacing of 2.0m and a row spacing of 2.0m, and each yellow silk grass was planted with verticillium. The initial weight of male crabs used in the experiment was about 27.78±1.44g, and that of female crabs was 22.05±2.02g. 1100 crabs were stocked in each pond, with a male-female ratio of 1:3. Each pond was surrounded by a plastic board (thickness 1.0mm, height 40cm) to prevent escape, and each inlet and outlet of the pond was filtered with a nylon net (mesh size 0.25mm) to exclude potential predators from the water source and drainage ditch.

[0055] (2) Fifteen days before the test, the fish were fed with compound feed once every three days, with 2.5 kg of compound feed per pond, for adaptive feeding. During the formal test, the control group was fed 2.5 kg of ice fish and 0.5 kg of compound feed in each pond every two days. After the first shelling, ice fish were fed once a day, with a proportion of 3.5% of the total biomass. In addition to the same feeding method as the control group, the experimental group was fed 2.5 kg of feed additives every half month. Experimental groups S1-S7 were fed the feed additives prepared in Examples 1-3 and Comparative Examples 1-4, respectively (the total bacterial viability in the feed additives was 1×10 9CFU / g), and feed at 17:00 during the entire experiment. Aquatic plant management was done well to ensure a reasonable density and space. Water temperature, pH value and dissolved oxygen were monitored twice a day at 9:00 and 17:00. A microporous oxygenation system was used to ensure sufficient dissolved oxygen (≥5.0mg / L). The pond water depth was controlled at 50-60cm from March to May, and at 80-100cm from June to October. During the breeding experiment, the water temperature was 14.0-32.8℃, the pH was 7.4-9.0, and the dissolved oxygen was 5.02-12.14 mg / L.

[0056] (3) Sample collection: Sampling was carried out in the second, sixth and tenth weeks, respectively. The crabs were fasted for 24 hours before sampling. Three male crabs and three female crabs were randomly selected from each parallel. After anesthesia on ice, the wet weight of the crabs was measured (accurate to 0.01g). 1.5mL of hemolymph was extracted from the joint membrane of the third foot base of the crabs, placed in a 4℃ refrigerator overnight, and centrifuged at 12000 rpm for 10min at 4℃. The supernatant (serum) was used for the later determination of serum biochemical indicators and antioxidant indicators. Then the muscles (T5) were carefully dissected and weighed. The meat yield (MY) was calculated according to the following formula: MY(%) = 100×muscle weight / body weight, and the meat yield was calculated, and the average body weight of each parallel was recorded.

[0057] (4) Evaluation indicators:

[0058] (4.1) Determination of growth performance related indicators:

[0059] (4.1.1) Serum biochemical indices: Glutamate aminotransferase (ALT), aspartate aminotransferase (AST), total protein (TP), albumin (ALP) and other indicators were measured using analytical kits (purchased from Nanjing Jiancheng Bioengineering Institute). Detailed operations were performed according to the kit instructions.

[0060] (4.1.2) Analysis of serum antioxidant properties: The superoxide dismutase (SOD) activity and malondialdehyde (MDA) content of serum were determined using the kits of Nanjing Jiancheng Bioengineering Institute.

[0061] (4.2) Determination of free amino acid content in crabs:

[0062] Extraction of free amino acids: Accurately weigh 2 g of crab sample (crab meat), add 10 mL of 8% sulfosalicylic acid solution to homogenize, precipitate at 4°C for half an hour, centrifuge at 4°C, take the supernatant, repeat the centrifugation once, and pass the extract through a 0.45 μm microporous filter membrane for testing.

[0063] Separation and determination of free amino acids: The extract was separated and determined by Sykam S-433D amino acid analyzer. The free amino acids in the sample were separated and determined by Li +The sulfonic acid group strong acid cation exchange resin is eluted one by one by the eluent, and the ninhydrin column is derivatized. After the reaction, the mixture flows into the dual-channel photometer for detection. Instrument operating conditions: S2100 pressure 39.0bar, total flow rate 0.45ml / min, S4300 reactor flow rate, 0.25ml / min, column temperature 60℃, pressure 6.0bar, reactor temperature 130℃.

[0064] (4.3) Sensory quality evaluation of river crabs:

[0065] After the crab farming was completed, the crabs were washed and steamed for 20 minutes before sensory evaluation. The males and females were separated. The sensory evaluation panel consisted of 20 participants who were not trained. The participants fasted for 1 hour before the evaluation.

[0066] (4.4) Determination of per-acre yield and catch rate of river crab farming:

[0067] After 8 months of crab farming, the crabs in each pond were harvested, the number and total weight were measured, and the per-acre yield and catch rate were calculated.

[0068] (5) Test results:

[0069] (5.1) Impact on crab growth indicators:

[0070] The results are shown in Table 1. The weight of male and female crabs in the experimental group was slightly higher than that of the control group during the entire breeding period, indicating that the composite bacterial agent can promote the growth of river crabs. When the S4 group replaced Bacillus coagulans BC66 with the commercially available bacteria ATCC7050, the weight of male and female crabs decreased compared with the S1 group; when the S5, S6 and S7 groups did not contain any of the three probiotics, Lactobacillus acidophilus LA71, Lactobacillus plantarum LP194 and Bacillus coagulans BC66, the weight of male and female crabs was significantly lower than that of S1, indicating that the three probiotics, Bacillus coagulans BC66, Lactobacillus plantarum LP194 powder and Lactobacillus acidophilus LA71, have a synergistic effect in promoting the growth of river crabs.

[0071] Table 1

[0072]

[0073] (5.2) Effects on serum biochemical parameters of river crabs:

[0074] The results are shown in Tables 2 and 3. It can be seen that the total protein content in the blood serum of river crabs is showing a rising trend as the crabs grow. The total protein content in the serum of the experimental group increases more than that of the control group as the culture time increases, indicating that the composite bacterial agent can increase the total protein content in the serum, but has no significant effect on the serum albumin in river crabs. Glutamate aminotransferase and aspartate aminotransferase are important indicators for measuring liver function. The glutamate aminotransferase and aspartate aminotransferase in the river crabs in the experimental group were significantly reduced compared with the second week, indicating that the composite bacterial agent can promote the maintenance of normal hepatopancreas function and improve the digestion and metabolism of the hepatopancreas to a certain extent. Moreover, the effect of the combination of BC66, LP194 and LA71 strains is more prominent, with a synergistic effect.

[0075] Table 2

[0076]

[0077] Table 3

[0078]

[0079] (5.3) Effects on serum antioxidant indexes of river crabs:

[0080] Total superoxide dismutase (T-SOD) is a relatively important antioxidant enzyme in the body's antioxidant defense system. Total superoxide dismutase (T-SOD) can convert superoxide anion free radicals into hydrogen peroxide and oxygen, thereby achieving the effect of removing superoxide anion free radicals. Excessive reactive oxygen free radicals can cause the oxidation of polyunsaturated fatty acids in the cell membrane to form a series of lipid peroxidation products. Malondialdehyde (MDA) is one of the products of lipid peroxidation. Its content in cells can measure the degree of cell damage and lipid peroxidation.

[0081] The results are shown in Table 4. It can be seen that as the crabs grow, the SOD activity of the control group and the experimental group gradually decreases, and there is no significant difference between the two in each time period. In the experimental group, as the feeding time increases, the MDA content is significantly lower than that of the control group, indicating that the composite bacterial agent can stabilize the MDA content in serum to a certain extent and reduce the occurrence of oxidative stress damage. Moreover, the effect of the combination of BC66 strain, LP194 strain and LA71 strain is more prominent, with a synergistic effect.

[0082] Table 4

[0083]

[0084] (5.4) Effects on free amino acid content in crabs:

[0085] Free amino acids (FAAs) are important flavor substances in food. The content of free amino acids has an important influence on the flavor of food. Amino acids have different tastes such as bitterness, sweetness, sourness and umami. Different amino acid compositions can be combined into a unique flavor of food. Therefore, the free amino acid analysis and evaluation method has also been applied to the study of river crab flavor. After the river crab farming is completed, the free amino acid content of the male river crab meat is analyzed.

[0086] The results are shown in Table 5. The total amount of free amino acids in the experimental group was higher than that in the control group. The content of amino acids with sweet taste characteristics such as arginine, alanine, proline, and glycine in the muscle was also relatively higher, indicating that the composite bacterial agent can improve the overall flavor of river crabs by affecting the free amino acid content in the body. The effect is more prominent when the BC66 strain, LP194 strain, and LA71 strain are used together, with a synergistic effect.

[0087] Table 5

[0088]

[0089] (5.5) Impact on sensory quality of river crabs:

[0090] The sensory evaluation criteria for river crabs are based on five aspects: color (the crab shell is shiny and has no spots), plumpness (the crab roe is rich and oily), fishy smell (it has a special fishy smell), meat quality (the crab meat is delicate and elastic) and sweetness (the flavor is fresh and sweet). The evaluation adopts a 1-5 scoring system. The higher the score, the more it likes it or the sensory evaluation index is extremely obvious. The lower the score, the more it dislikes it or the sensory evaluation index is not obvious.

[0091] The results are shown in Table 6. There was no difference in color between the experimental group and the control group, and the overall evaluation was relatively close. The experimental group was better in terms of freshness and sweetness, and the comprehensive score was also higher. The effect was more prominent when the BC66 strain, LP194 strain and LA71 strain were used together, and had a synergistic effect, which was related to the fact that the compound probiotics could remove the earthy smell and increase the free amino acid content.

[0092] Table 6

[0093]

[0094] (5.6) Impact on river crab farming results:

[0095] The results are as follows Figure 1 , Figure 2 As shown in the results, regular use of compound bacterial agents can reduce crab mortality, increase recapture rate, increase per-acre yield, and improve crab farming results. The combined use of BC66, LP194, and LA71 strains has a more prominent effect, with a synergistic effect.

[0096] The applicant declares that the present invention illustrates the technical solution of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0097] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0098] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. Use of a feed additive in preparing a product for promoting the growth and development of Chinese mitten crab and improving the meat quality and flavor of Chinese mitten crab, characterized in that: The strain in the feed additive is Bacillus coagulans with a deposit number of CGMCC 21879. Bacillus coagulans BC66 strain, Lactobacillus plantarum with the deposit number CGMCC 21766 Lactobacillus plantarum LP194 strain and Lactobacillus acidophilus with a deposit number of CGMCC 21765 Lactobacillus acidophilus LA71 strain composition; The ratio of the viable counts of the BC66 strain, the LP194 strain, and the LA71 strain is (1-10):(1-10):(1-10); The total bacterial count in the feed additive is not less than 1×10 9 CFU / mL or 1×10 9 CFU / g.

2. The use according to claim 1, characterized in that: The dosage form of the feed additive includes freeze-dried powder, tablet, granule or solution.

3. The use according to claim 2, characterized in that: The dosage form of the feed additive is a freeze-dried powder, which is prepared by a preparation method comprising the following steps: The BC66 strain, the LP194 strain and the LA71 strain are inoculated into a culture medium respectively, activated and fermented in sequence to obtain a fermentation broth; the fermentation broth is centrifuged respectively, mixed with a freeze-drying protective agent and freeze-dried to obtain BC66 bacterial powder, LP194 bacterial powder and LA71 bacterial powder; the BC66 bacterial powder, LP194 bacterial powder and LA71 bacterial powder are mixed according to a ratio to obtain the feed additive.

4. The use according to claim 3, characterized in that: The lyoprotectant is selected from any one or a combination of at least two of skim milk, gelatin, dextrin, gum arabic, dextran, sodium alginate, polyvinyl pyrrolidone, sucrose, lactose, trehalose, sorbitol or xylitol.

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