Composition for promoting healthy growth of bullfrog and preparation method thereof

Abstract

The present invention relates to a composition for promoting the healthy growth of bullfrogs and a preparation method thereof, and belongs to the technical field of feed additives. The present invention creatively utilizes Aspergillus oryzae and neutral protease to carry out bacterial enzyme synergistic fermentation on soybean meal, thereby preparing fermented soybean meal, and then a certain mass ratio of dandelion extract, chuanxiong extract and Centella asiatica extract are used to form a composite Chinese herbal medicine, and a certain mass ratio of astaxanthin and β-1,3-d-glucan are used to form a composite regulator, so that when the composition obtained by mixing the three components with other components is added to feed and applied to the daily feeding of bullfrogs, on the one hand, the three components can exert a good synergistic effect, effectively reducing the illness of bullfrogs, and on the other hand, the composition also plays an excellent role in promoting the growth and development of bullfrogs, achieving healthy growth.

Description

Technical Field

[0001] The invention belongs to the technical field of feed additives, and particularly relates to a composition for promoting the healthy growth of bullfrogs and a preparation method thereof. Background Art

[0002] Bullfrog is a large edible frog with tender meat, rich nutrition and certain medicinal value. With its many advantages such as fast reproduction, strong adaptability, wide diet and delicious taste, the market scale of bullfrog is expanding rapidly. However, as the market demand for bullfrog continues to increase, problems are also coming one after another. In order to maximize profits, many breeding companies will continue to increase the breeding density of bullfrogs, resulting in a very poor breeding environment. This practice not only reduces the activity space of bullfrogs and seriously hinders their growth rate, but also due to the large-scale breeding of harmful microorganisms such as bacteria and viruses, bullfrogs are also very susceptible to disease and even death. Death, in this case, it is common to use various drugs mainly antibiotics for breeding. In order to solve this problem, many existing technologies will not only improve the breeding environment, but also optimize and adjust the bullfrog feed formula. By adding feed additives, the growth and development of bullfrogs can be promoted. Under the same breeding conditions, the meat of bullfrogs can be fatter. However, these feed additives still have disadvantages that limit their application effect. This is because most of these feed additives still cannot reduce the diseases of bullfrogs, such as red leg disease, ascites, etc., and still have a great restrictive effect on the growth of bullfrogs. Summary of the invention

[0003] In view of the problems existing in the prior art, the purpose of the present invention is to provide a composition for promoting the healthy growth of bullfrogs and a preparation method thereof. The present invention creatively utilizes Aspergillus oryzae and neutral protease to carry out bacterial enzyme synergistic fermentation on soybean meal, thereby preparing fermented soybean meal, and then a dandelion extract, a chuanxiong extract and a Centella asiatica extract in a certain mass ratio are used to form a composite Chinese herbal medicine, and a certain mass ratio of astaxanthin and β-1,3-d-glucan are used to form a composite regulator, so that when the composition obtained by mixing the three with other components is added to feed and applied to the daily feeding of bullfrogs, on the one hand, the three can exert a good synergistic effect and effectively reduce the disease of bullfrogs, and on the other hand, the composition also plays an excellent promoting role in the growth and development of bullfrogs, thereby achieving healthy growth.

[0004] The purpose of the present invention can be achieved through the following technical solutions:

[0005] A method for preparing a composition for promoting the healthy growth of bullfrogs, the preparation method comprising the following steps:

[0006] The preparation is completed by mixing 45-70 parts by weight of fermented soybean meal, 0.5-1 parts by weight of complex vitamins, 0.5-1 parts by weight of complex trace elements, 1.5-2 parts by weight of complex Chinese herbal medicines, 0.3-0.5 parts by weight of attractants, 2-2.5 parts by weight of complex regulators and 20-50 parts by weight of carriers under stirring at room temperature for 5-10 minutes.

[0007] As a preferred technical solution of the present invention, the fermented soybean meal is prepared by the following steps:

[0008] Step A: adding deionized water to 5-8 parts by weight of pretreated soybean meal, sterilizing, and naturally cooling to room temperature to obtain material A;

[0009] Step B: adding 1500-2000U / g of neutral protease to the material A and inoculating 15000-20000cfu / g of Aspergillus oryzae, and then standing and fermenting at 30-35°C for 70-74h to obtain a fermented material;

[0010] Step C: drying the fermented material, and then crushing it to be able to pass through a 40-100 mesh sieve, and the preparation is completed.

[0011] Furthermore, the pretreatment of soybean meal in step A refers to crushing the soybean meal to pass through a 30-50 mesh sieve.

[0012] Furthermore, the sterilization in step A refers to sterilization at 105° C. for 15-20 minutes.

[0013] Furthermore, the moisture content of material A in step A is 35-40wt%.

[0014] Furthermore, the drying in step C refers to drying at 70-80° C. to constant weight.

[0015] As a preferred technical solution of the present invention, the complex vitamin is mixed by 10-12 parts by weight of vitamin B1, 1-3 parts by weight of vitamin B12, 10-12 parts by weight of vitamin K3, 16-30 parts by weight of vitamin A, 8-13 parts by weight of vitamin D3, 50-60 parts by weight of niacin, 2-3 parts by weight of folic acid, 8-10 parts by weight of riboflavin, 30-50 parts by weight of pantothenic acid and 40-60 parts by weight of inositol.

[0016] As a preferred technical solution of the present invention, the composite trace elements are mixed by 150-200 parts by weight of potassium chloride, 50-60 parts by weight of calcium iodate, 55-65 parts by weight of copper sulfate, 80-100 parts by weight of cobalt sulfate, 100-200 parts by weight of ferrous sulfate, 75-90 parts by weight of zinc sulfate, 50-60 parts by weight of manganese sulfate, 200-400 parts by weight of magnesium sulfate, 20-30 parts by weight of sodium selenite and 400-500 parts by weight of zeolite powder.

[0017] As a preferred technical solution of the present invention, the composite Chinese herbal medicine comprises the following components: dandelion extract, chuanxiong extract and Centella asiatica extract; the mass ratio of the dandelion extract, the chuanxiong extract and the Centella asiatica extract is 3-4:1-1.5:2-4.

[0018] As a preferred technical solution of the present invention, the attractant is dimethyl-β-thiazolinone.

[0019] As a preferred technical solution of the present invention, the composite regulator comprises the following components: astaxanthin and β-1,3-d-glucan; the mass ratio of the astaxanthin to the β-1,3-d-glucan is 1-3:1-1.5.

[0020] As a preferred technical solution of the present invention, the carrier is rice husk powder.

[0021] A composition for promoting the healthy growth of bullfrogs prepared by the preparation method as described above.

[0022] As a preferred technical solution of the present invention, the composition is added in an amount of 5-10wt% in feed.

[0023] Beneficial effects of the present invention:

[0024] (1) The present invention creatively utilizes Aspergillus oryzae and neutral protease to carry out bacterial enzyme synergistic fermentation on soybean meal, thereby preparing fermented soybean meal, and then a certain mass ratio of dandelion extract, Chuanxiong extract and Centella asiatica extract are used to form a composite Chinese herbal medicine, and a certain mass ratio of astaxanthin and β-1,3-d-glucan are used to form a composite regulator, so that when the three components are mixed with other components and the resulting composition is added to the feed and applied to the daily feeding of bullfrogs, on the one hand, the three components can exert a good synergistic effect and effectively reduce the disease of bullfrogs, and on the other hand, the composition also plays an excellent role in promoting the growth and development of bullfrogs, thereby achieving healthy growth.

[0025] (2) The present invention creatively utilizes Aspergillus oryzae and neutral protease to carry out bacterial enzyme synergistic fermentation on soybean meal, so that the fermented soybean meal can further reduce the anti-nutritional factors contained therein and increase the content of nutrients such as small molecule amino acids and peptides that are easily absorbed and utilized, thereby ensuring a high utilization rate of protein when bullfrogs ingest it, promoting growth and development and appetite, and at the same time, avoiding allergies, diseases, and digestion inhibition caused by anti-nutritional factors as much as possible. On this basis, the present invention compounded a composite Chinese herbal medicine composed of dandelion extract, chuanxiong extract, and Centella asiatica extract in a certain mass ratio, through The effect of the composite Chinese herbal medicine can achieve the purpose of improving immunity and promoting appetite. Dandelion is rich in various trace elements and vitamins, and has good antibacterial and anti-inflammatory effects and can improve immunity. Chuanxiong can promote digestion and regulate gastrointestinal motility. The synergistic composite vitamins and composite trace elements can be better absorbed. The various biologically active substances contained in Centella asiatica can also promote the metabolism of bullfrogs, thereby improving the overall utilization rate of feed. In addition, the present invention also adds astaxanthin and β-1,3-d-glucan with excellent anti-inflammatory and antioxidant effects to reduce the probability of inflammation.

[0026] (3) The present invention creatively prepares a composition by rationally matching the various components, so that after being added to the feed, it can produce an excellent synergistic effect and promote the healthy growth of bullfrogs. DETAILED DESCRIPTION

[0027] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the specific implementation methods, structures, features and effects of the present invention are described in detail below in combination with the embodiments.

[0028] The soybean meal in all embodiments and comparative examples of the present invention is purchased directly from the market, and the pure soybean meal purchased from Dongyi Yang (Jinan) New Materials Co., Ltd. is item number 2561; the dandelion extract is purchased directly from the market, and is purchased from Shaanxi Guben Biotechnology Co., Ltd., item number GBPGYF-381; the Centella asiatica extract is purchased directly from the market, and is purchased from Shaanxi Guben Biotechnology Co., Ltd., item number GBSWJXCD-205; the Chuanxiong extract is purchased directly from the market, and is purchased from Shaanxi Boundary Biotechnology Co., Ltd., single product number BJ08; dimethyl-β-propionic acid thiazolinone is purchased directly from the market, and is purchased from Wuhan Guanying Biotechnology Co., Ltd.; β-1,3-d-glucan is purchased directly from the market, and is purchased from Xuzhou Saifu Biotechnology Co., Ltd., with a product content of 90%; neutral protease is purchased directly from the market, and is purchased from Beijing Solebow Technology Co., Ltd., item number Z8031; the source of Aspergillus oryzae is the China Industrial Microbiological Culture Collection Management Center, strain number CICC 2339.

[0029] Example 1

[0030] A method for preparing a composition for promoting the healthy growth of bullfrogs, the preparation method comprising the following steps:

[0031] 45 parts by weight of fermented soybean meal, 0.5 parts by weight of complex vitamins, 0.5 parts by weight of complex trace elements, 1.5 parts by weight of complex Chinese herbal medicine, 0.3 parts by weight of attractant, 2 parts by weight of complex regulator and 20 parts by weight of carrier are mixed and stirred at room temperature for 5 minutes to complete the preparation.

[0032] The fermented soybean meal is prepared by the following steps:

[0033] Step A: adding deionized water to 5 parts by weight of pretreated soybean meal, sterilizing, and naturally cooling to room temperature to obtain material A;

[0034] Step B: adding 1500U / g of neutral protease to the material A and inoculating 15000cfu / g of Aspergillus oryzae, and then standing and fermenting at 30°C for 70h to obtain a fermented material;

[0035] Step C: Dry the fermented material, and then crush it until it can pass through a 40-mesh sieve, and the preparation is completed.

[0036] The pretreatment of soybean meal in step A refers to crushing the soybean meal to a size that can pass through a 30-mesh sieve.

[0037] The sterilization in step A refers to sterilization at 105° C. for 15 minutes.

[0038] The moisture content of material A in step A is 35wt%.

[0039] The drying in step C refers to drying at 70° C. to constant weight.

[0040] The complex vitamin is prepared by mixing 10 parts by weight of vitamin B1, 1 part by weight of vitamin B12, 10 parts by weight of vitamin K3, 16 parts by weight of vitamin A, 8 parts by weight of vitamin D3, 50 parts by weight of niacin, 2 parts by weight of folic acid, 8 parts by weight of riboflavin, 30 parts by weight of pantothenic acid and 40 parts by weight of inositol.

[0041] The composite trace elements are prepared by mixing 150 parts by weight of potassium chloride, 50 parts by weight of calcium iodate, 55 parts by weight of copper sulfate, 80 parts by weight of cobalt sulfate, 100 parts by weight of ferrous sulfate, 75 parts by weight of zinc sulfate, 50 parts by weight of manganese sulfate, 200 parts by weight of magnesium sulfate, 20 parts by weight of sodium selenite and 400 parts by weight of zeolite powder.

[0042] The composite Chinese herbal medicine comprises the following components: dandelion extract, chuanxiong extract and centella asiatica extract; the mass ratio of the dandelion extract, the chuanxiong extract and the centella asiatica extract is 3:1:2.

[0043] The attractant is dimethyl-β-thiazolinone.

[0044] The composite regulator comprises the following components: astaxanthin and β-1,3-d-glucan; the mass ratio of the astaxanthin to the β-1,3-d-glucan is 1:1.

[0045] The carrier is rice husk powder.

[0046] A composition for promoting the healthy growth of bullfrogs prepared by the preparation method as described above.

[0047] Example 2

[0048] A method for preparing a composition for promoting the healthy growth of bullfrogs, the preparation method comprising the following steps:

[0049] The preparation is completed by mixing 70 parts by weight of fermented soybean meal, 1 part by weight of complex vitamins, 1 part by weight of complex trace elements, 2 parts by weight of complex Chinese herbal medicines, 0.5 parts by weight of attractants, 2.5 parts by weight of complex regulators and 50 parts by weight of carriers at room temperature under stirring for 10 minutes.

[0050] The fermented soybean meal is prepared by the following steps:

[0051] Step A: adding deionized water to 8 parts by weight of pretreated soybean meal, sterilizing, and naturally cooling to room temperature to obtain material A;

[0052] Step B: adding 2000U / g of neutral protease to the material A and inoculating 20000cfu / g of Aspergillus oryzae, and then standing and fermenting at 35°C for 74h to obtain a fermented material;

[0053] Step C: Dry the fermented material, and then crush it until it can pass through a 100-mesh sieve, and the preparation is completed.

[0054] The pretreatment of soybean meal in step A refers to crushing the soybean meal to a size that can pass through a 50-mesh sieve.

[0055] The sterilization in step A refers to sterilization at 105° C. for 20 minutes.

[0056] The moisture content of material A in step A is 40wt%.

[0057] The drying in step C refers to drying at 80° C. to constant weight.

[0058] The complex vitamin is prepared by mixing 12 parts by weight of vitamin B1, 3 parts by weight of vitamin B12, 12 parts by weight of vitamin K3, 30 parts by weight of vitamin A, 13 parts by weight of vitamin D3, 60 parts by weight of niacin, 3 parts by weight of folic acid, 10 parts by weight of riboflavin, 50 parts by weight of pantothenic acid and 60 parts by weight of inositol.

[0059] The composite trace elements are prepared by mixing 200 parts by weight of potassium chloride, 60 parts by weight of calcium iodate, 65 parts by weight of copper sulfate, 100 parts by weight of cobalt sulfate, 200 parts by weight of ferrous sulfate, 90 parts by weight of zinc sulfate, 60 parts by weight of manganese sulfate, 400 parts by weight of magnesium sulfate, 30 parts by weight of sodium selenite and 500 parts by weight of zeolite powder.

[0060] The composite Chinese herbal medicine comprises the following components: dandelion extract, chuanxiong extract and centella asiatica extract; the mass ratio of the dandelion extract, the chuanxiong extract and the centella asiatica extract is 4:1.5:4.

[0061] The attractant is dimethyl-β-thiazolinone.

[0062] The composite regulator comprises the following components: astaxanthin and β-1,3-d-glucan; the mass ratio of the astaxanthin to the β-1,3-d-glucan is 3:1.5.

[0063] The carrier is rice husk powder.

[0064] A composition for promoting the healthy growth of bullfrogs prepared by the preparation method as described above.

[0065] Example 3

[0066] A method for preparing a composition for promoting the healthy growth of bullfrogs, the preparation method comprising the following steps:

[0067] 60 parts by weight of fermented soybean meal, 0.7 parts by weight of complex vitamins, 0.8 parts by weight of complex trace elements, 1.8 parts by weight of complex Chinese herbal medicine, 0.4 parts by weight of attractant, 2.3 parts by weight of complex regulator and 45 parts by weight of carrier are stirred and mixed at room temperature for 8 minutes to complete the preparation.

[0068] The fermented soybean meal is prepared by the following steps:

[0069] Step A: adding deionized water to 7 parts by weight of pretreated soybean meal, sterilizing, and naturally cooling to room temperature to obtain material A;

[0070] Step B: adding 1800U / g of neutral protease to the material A and inoculating 18000cfu / g of Aspergillus oryzae, and then standing and fermenting at 33°C for 72h to obtain a fermented material;

[0071] Step C: Dry the fermented material, and then crush it until it can pass through a 60-mesh sieve, and the preparation is completed.

[0072] The pretreatment of soybean meal in step A refers to crushing the soybean meal to pass through a 40-mesh sieve.

[0073] The sterilization in step A refers to sterilization at 105°C for 18 minutes.

[0074] The moisture content of material A in step A is 38wt%.

[0075] The drying in step C refers to drying at 75° C. to constant weight.

[0076] The complex vitamin is prepared by mixing 11 parts by weight of vitamin B1, 2 parts by weight of vitamin B12, 11 parts by weight of vitamin K3, 20 parts by weight of vitamin A, 10 parts by weight of vitamin D3, 55 parts by weight of niacin, 2.5 parts by weight of folic acid, 9 parts by weight of riboflavin, 40 parts by weight of pantothenic acid and 50 parts by weight of inositol.

[0077] The composite trace elements are prepared by mixing 180 parts by weight of potassium chloride, 55 parts by weight of calcium iodate, 60 parts by weight of copper sulfate, 90 parts by weight of cobalt sulfate, 150 parts by weight of ferrous sulfate, 80 parts by weight of zinc sulfate, 55 parts by weight of manganese sulfate, 300 parts by weight of magnesium sulfate, 25 parts by weight of sodium selenite and 450 parts by weight of zeolite powder.

[0078] The composite Chinese herbal medicine comprises the following components: dandelion extract, chuanxiong extract and centella asiatica extract; the mass ratio of the dandelion extract, the chuanxiong extract and the centella asiatica extract is 3.5:1.3:3.

[0079] The attractant is dimethyl-β-thiazolinone.

[0080] The composite regulator comprises the following components: astaxanthin and β-1,3-d-glucan; the mass ratio of the astaxanthin to the β-1,3-d-glucan is 2:1.3.

[0081] The carrier is rice husk powder.

[0082] A composition for promoting the healthy growth of bullfrogs prepared by the preparation method as described above.

[0083] Comparative Example 1

[0084] On the basis of Example 1, fermented soybean meal was replaced with soybean meal of equal weight, and the rest remained unchanged.

[0085] Comparative Example 2

[0086] Based on Example 1, the neutral protease in step B was changed to an equal weight of Aspergillus oryzae, and the rest remained unchanged.

[0087] Comparative Example 3

[0088] On the basis of Example 1, in step B, Aspergillus oryzae was replaced with an equal weight of neutral protease, and the rest remained unchanged.

[0089] Comparative Example 4

[0090] On the basis of Example 1, the dandelion extract was replaced with an equal weight of Chuanxiong extract, and the rest remained unchanged.

[0091] Comparative Example 5

[0092] On the basis of Example 1, the extract of Chuanxiong was replaced by an equal weight of Centella asiatica extract, and the rest remained unchanged.

[0093] Comparative Example 6

[0094] On the basis of Example 1, the Centella asiatica extract was replaced with an equal weight of Taraxacum officinale extract, and the rest remained unchanged.

[0095] Comparative Example 7

[0096] On the basis of Example 1, astaxanthin was replaced by an equal weight of β-1,3-d-glucan, and the rest remained unchanged.

[0097] Comparative Example 8

[0098] On the basis of Example 1, β-1,3-d-glucan was replaced with astaxanthin of equal weight, and the rest remained unchanged.

[0099] Comparative Example 9

[0100] On the basis of Example 1, the compound Chinese herbal medicine is replaced with a compound regulator of equal weight, and the rest remains unchanged.

[0101] Comparative Example 10

[0102] On the basis of Example 1, the composite regulator was changed to a composite Chinese herbal medicine of equal weight, and the rest remained unchanged.

[0103] Test Example 1

[0104] Breeding effect test:

[0105] Here are the steps:

[0106] (1) 1400 healthy American bullfrogs with an initial body weight of 7.8-8.0 g were selected and randomly divided into 14 groups, with 100 frogs in each group.

[0107] (2) Adding the compositions for promoting healthy growth of bullfrogs prepared in Examples 1-3 and Comparative Examples 1-10 (the addition amount is 8 wt%) to the same feed, and then feeding each group respectively (one group is a blank group, without adding the composition, and directly feeding the feed), to test the breeding effect, wherein the breeding density is 100 / m 2 The water level was 40 cm, the water temperature was 25 °C, and the fish were fed twice a day with the same feeding amount for each group (once at 10:00 and once at 18:00). The test period was 90 days.

[0108] (3) During the test period, the number of bullfrogs with diseases in each group was counted, with red leg disease, ascites and gastroenteritis as typical diseases (if the same bullfrog had multiple diseases, the number was still recorded as 1). At the same time, after the test period, the bullfrogs in each group except those with diseases were weighed (i.e., the sick bullfrogs were not counted), and the average weight gain rate was calculated.

[0109] Table 1. Breeding effect test results

[0110] Number of sick Average weight gain rate / % Example 1 4 2109 Example 2 2 2128 Example 3 1 2087 Comparative Example 1 18 1831 Comparative Example 2 10 1949 Comparative Example 3 14 1895 Comparative Example 4 11 2012 Comparative Example 5 8 1991 Comparative Example 6 9 1980 Comparative Example 7 10 2003 Comparative Example 8 9 2000 Comparative Example 9 9 1994 Comparative Example 10 10 2020 Blank Group 23 1795

[0111] From the comparison of Test Example 1, Example 1, Comparative Examples 1-10 and the blank group, it can be seen that the composition prepared by the present invention can well promote the healthy growth of bullfrogs and reduce the diseases of bullfrogs when added to feed for feeding.

[0112] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A method for preparing a composition for promoting the healthy growth of bullfrogs, characterized in that: The preparation method comprises the following steps: 45-70 parts by weight of fermented soybean meal, 0.5-1 parts by weight of complex vitamins, 0.5-1 parts by weight of complex trace elements, 1.5-2 parts by weight of complex Chinese herbal medicine, 0.3-0.5 parts by weight of attractant, 2-2.5 parts by weight of complex regulator and 20-50 parts by weight of carrier are mixed and stirred at room temperature for 5-10 minutes to complete the preparation; The fermented soybean meal is prepared by the following steps: Step A: adding deionized water to 5-8 parts by weight of pretreated soybean meal, sterilizing, and naturally cooling to room temperature to obtain material A; Step B: adding 1500-2000U / g of neutral protease to the material A and inoculating 15000-20000cfu / g of Aspergillus oryzae, and then standing and fermenting at 30-35°C for 70-74h to obtain a fermented material; Step C: drying the fermented material, and then crushing it to pass through a 40-100 mesh sieve, and the preparation is completed; The composite Chinese herbal medicine comprises the following components: dandelion extract, chuanxiong extract and centella asiatica extract; the mass ratio of the dandelion extract, the chuanxiong extract and the centella asiatica extract is 3-4:1-1.5:2-4; The composite regulator comprises the following components: astaxanthin and β-1,3-d-glucan; the mass ratio of the astaxanthin to the β-1,3-d-glucan is 1-3:1-1.

5.

2. The method for preparing a composition for promoting the healthy growth of bullfrog according to claim 1, characterized in that: The moisture content of material A in step A is 35-40wt%.

3. The method for preparing a composition for promoting the healthy growth of bullfrog according to claim 1, characterized in that: The complex vitamin is prepared by mixing 10-12 parts by weight of vitamin B1, 1-3 parts by weight of vitamin B12, 10-12 parts by weight of vitamin K3, 16-30 parts by weight of vitamin A, 8-13 parts by weight of vitamin D3, 50-60 parts by weight of niacin, 2-3 parts by weight of folic acid, 8-10 parts by weight of riboflavin, 30-50 parts by weight of pantothenic acid and 40-60 parts by weight of inositol.

4. The method for preparing a composition for promoting the healthy growth of bullfrog according to claim 1, characterized in that: The composite trace elements are prepared by mixing 150-200 parts by weight of potassium chloride, 50-60 parts by weight of calcium iodate, 55-65 parts by weight of copper sulfate, 80-100 parts by weight of cobalt sulfate, 100-200 parts by weight of ferrous sulfate, 75-90 parts by weight of zinc sulfate, 50-60 parts by weight of manganese sulfate, 200-400 parts by weight of magnesium sulfate, 20-30 parts by weight of sodium selenite and 400-500 parts by weight of zeolite powder.

5. The method for preparing a composition for promoting the healthy growth of bullfrog according to claim 1, characterized in that: The attractant is dimethyl-β-thiazolinone.

6. The method for preparing a composition for promoting the healthy growth of bullfrog according to claim 1, characterized in that: The carrier is rice husk powder.

7. A composition for promoting the healthy growth of bullfrog prepared by the preparation method according to any one of claims 1 to 6.