A natural skin care hydrogel for pet wounds and its preparation method and application

Abstract

The present invention provides a natural skin care hydrogel for pet wounds and a preparation method and application thereof, wherein the raw materials for preparing the hydrogel include: a gel forming agent, a moisturizing agent, aloe polysaccharide, zinc hyaluronate, saccharic acid, laurocapron, zinc gluconate, vitamin E, vitamin B, PEG-40 castor oil, polyethylene glycol, triethanolamine, mint essence, ethyl hydroxybenzoate and deionized water. The natural hydrogel matrix screened out by the present invention can absorb and retain a large amount of water to form a soft, elastic and transparent gel-like substance; the gel has a uniform appearance and has the dual effects of hydration and absorption, and has good gel network mechanical properties, density, apparent viscosity, water absorption and moisturizing air permeability, can keep the wound surface moist and have the dual effects of hydration and absorption, autonomously moisturize the wound surface to promote epidermal growth and epithelial regeneration, prolong the contact time with the skin, and improve the absorption of effective substances; the physicochemical properties are stable, and it is a very ideal skin dressing for external use.

Description

Technical Field

[0001] The invention belongs to the technical field of pharmaceutical preparations for pets, and particularly relates to a natural skin care hydrogel for pet wounds, and a preparation method and application thereof. Background Art

[0002] With the rapid development of the pet industry, pets have become important members of many families, and the health and well-being of pets have naturally become the focus of pet owners. In this context, the demand for pet care products, especially for specific needs such as wound care, continues to grow. At present, pet skin care products on the market are mainly skin sprays and emulsion ointments, which mainly rely on synthetic chemical ingredients and may cause pet skin sensitivity or long-term side effects. Among them, the skin spray has a relatively high moisture content and no adhesion to the skin, so it is difficult to achieve the effect of moisturizing the skin. At the same time, the spray texture is unstable and is more susceptible to the influence of external environmental temperature and humidity, which inhibits its efficacy. At the same time, the emulsion ointment is greasy, difficult to clean, weak in water absorption, and not easy to mix with water-soluble drugs. Hydrogel is a hydrophilic three-dimensional cross-linked network structure. Due to its special structure of internal cross-linking of molecules, it has high water absorption and water retention, can absorb and retain a large amount of water, and form a soft, elastic and transparent gel-like substance. When used in wound repair, hydrogels not only provide excellent moisturizing and biocompatibility, but also promote cell migration, accelerate wound repair, and increase the absorption concentration of effective substances. They are non-greasy, easy to spread and clean, and have a certain degree of adhesion, overcoming the shortcomings of sprays, oily and emulsion ointments.

[0003] There are many kinds of pet wound skin care products on the market, but they differ in ingredients and effects. General skin care gels only have moisturizing effects and single functions, and cannot meet the requirements of good gel network mechanical properties, density, water absorption, moisturizing and breathability. At the same time, many pet skin care products rely on synthetic chemical ingredients, which may cause pet skin sensitivity or long-term side effects. Although some products claim to use natural ingredients, they are often not fully tested, and their effectiveness and safety remain to be verified, which makes it difficult for pet owners to find effective wound care solutions. Summary of the invention

[0004] In view of the problems existing in the prior art, the purpose of the present invention is to provide a natural pet wound skin care hydrogel and a preparation method and application thereof. The raw materials for preparing the hydrogel do not contain chemical components, effectively promote wound healing, are non-irritating and non-sensitizing, and are suitable for long-term use by pets.

[0005] The objective of the present invention is achieved through the following technical solutions:

[0006] The first aspect of the present invention provides a natural pet wound skin care hydrogel, the hydrogel comprising the following raw materials in percentage by weight:

[0007] The composition comprises: gel forming agent 0.5-5%, moisturizing agent 10-25%, aloe polysaccharide 0.1-2%, zinc hyaluronate 0.5-4%, bird's nest acid 0.1-1%, lauryl azone 0.2-2%, zinc gluconate 0.1-0.5%, vitamin E 0.2-1%, vitamin B 0.1-1%, PEG-40 castor oil 0.5-3%, polyethylene glycol 0.5-5%, triethanolamine 0.25-5%, mint flavor 0.1-1%, ethyl hydroxybenzoate 0.05-0.1%, and the balance is deionized water. The sum of the mass percentages of the raw material components is 100%.

[0008] Furthermore, the ratio of the gel forming agent to triethanolamine is 1:0.5-2.

[0009] Furthermore, the gel forming agent is one or a combination of two or more of carbomer 934, carbomer 940, sodium carboxymethyl cellulose, sodium alginate, chitosan hydrochloride, hydroxypropyl cellulose, and hydroxypropyl methyl cellulose.

[0010] Furthermore, the moisturizing agent is one or a combination of two or more of glycerol, propylene glycol, collagen and sorbitol.

[0011] Furthermore, the purity of the aloe polysaccharide is not less than 60%. Aloe polysaccharide is a natural plant extract of aloe vera, which has the pharmacological effects of promoting wound healing and tissue repair, reducing scar formation, protecting mucous membranes, breathable and moisturizing, antibacterial, and anti-inflammatory.

[0012] Furthermore, the zinc hyaluronate is hydrolyzed zinc hyaluronate, and its molecular weight is 100-500 kDa, which is easy to disperse in water. Zinc hyaluronate is a combination of hyaluronic acid and zinc, which not only has the moisturizing, repairing and skin nourishing characteristics of hyaluronic acid, but also has the soothing, accelerating epidermal cell growth and division, antibacterial, anti-oxidation and anti-inflammatory effects of zinc.

[0013] Furthermore, the lauryl azone is water-soluble lauryl azone, and its purity is not less than 98%. Water-soluble lauryl azone is a new type of highly effective transdermal enhancer, which can increase the circulation of skin stratum corneum lipids and has the effect of promoting transdermal absorption. Lauryl azone can also increase the adhesion to the skin surface, so that the functional substance stays on the skin for a longer time, thereby improving the absorption effect.

[0014] The second aspect of the present invention provides a method for preparing the natural pet wound skin care hydrogel according to the first aspect, comprising the following steps:

[0015] (1) Weigh aloe polysaccharide in a certain amount of deionized water, stir under magnetic stirring at 40-50°C until completely dissolved, cool to room temperature to obtain aloe polysaccharide skin care liquid, and seal in a volumetric bottle for later use;

[0016] (2) Weigh zinc hyaluronate in a certain amount of deionized water, stir under magnetic stirring at 40-50°C until completely dissolved, cool to room temperature to obtain zinc hyaluronate skin care liquid, and seal in a volumetric flask for later use;

[0017] (3) Weigh a gel forming agent according to a certain proportion, add the gel forming agent into deionized water, stir magnetically for 1-3 hours, and then soak at room temperature for 10-14 hours to allow the gel forming agent to fully hydrate and swell, thereby obtaining liquid A;

[0018] (4) Weigh triethanolamine, laurocapram, mint essence, and ethylparaben in proportion, add them to deionized water, and sonicate at 50-60°C for 30-60 min to completely dissolve them to obtain solution B;

[0019] (5) Weigh sialic acid, zinc gluconate, vitamin E, vitamin B, PEG-40 castor oil and polyethylene glycol in proportion, add them to deionized water, stir magnetically at 60-70°C for 1-4 hours to completely dissolve them, and obtain liquid C;

[0020] (6) After adding moisturizer to liquid A and grinding evenly, slowly add it to liquid B, and stir and mix continuously for 1-3 hours until the hydrogel matrix is ​​formed. Then add liquid C, aloe polysaccharide skin care lotion and hyaluronic acid zinc skin care lotion in sequence, stir and mix evenly, and adjust the pH to 6.0-8.0 with NaOH solution to obtain the natural skin care hydrogel for pet wounds.

[0021] The third aspect of the present invention provides the use of the natural pet wound skin care hydrogel described in the first aspect in the preparation of pet wound repair products.

[0022] Furthermore, the pets include pet cats, pet dogs, pet rabbits and pet mice, and the products are wound repair care products or medicines.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] 1. The natural hydrogel matrix (hydrogel matrix formed by carbomer and triethanolamine) selected by the present invention can absorb and retain a large amount of water to form a soft, elastic and transparent gel-like substance; specifically, carbomer molecules contain many acidic groups, and alkali neutralization is required during the application process. When carbomer is neutralized with the alkaline substance triethanolamine, its molecular chain will extend in a greatly expanded state due to the mutual repulsion of negative charges, forming salts, so that the curled molecules are opened due to electrical repulsion, thereby increasing their volume and viscosity. This reaction usually adjusts the pH to about 6-8 to obtain a crystal clear hydrogel. At the same time, the three-dimensional network structure of the hydrogel provides a large amount of release space for the added functional substances (aloe polysaccharides, saccharic acid and transparent phytic acid zinc), and the functional substances wrapped in the hydrogel matrix are less affected by the surrounding environmental stress factors and conditions. In addition, this three-dimensional network structure is also conducive to the material exchange between the functional substances and the skin.

[0025] 2. The natural skin care hydrogel for pet wounds prepared by the present invention has a uniform appearance, has the dual functions of hydration and absorption, and has good gel network mechanical properties, density, apparent viscosity, water absorption, and moisturizing and breathability. It can keep the wound surface moist and has the dual functions of hydration and absorption. It can autonomously moisturize the wound surface to promote epidermal growth and epithelial regeneration, prolong the contact time with the skin, and improve the absorption of effective substances. The physical and chemical properties are stable at high and low temperatures, and it is an ideal skin dressing for external use, providing a new method and idea for treating pet wounds.

[0026] 3. The natural gel-forming agent added to the product of the present invention can form an effective protective film on the surface of pet skin, which can not only moisturize and repair the skin, but also resist the invasion of external microorganisms;

[0027] 4. The laurocapram added to the product of the present invention is a new type of highly effective transdermal enhancer, which can increase the circulation of lipids in the stratum corneum of pet skin and has the effect of promoting transdermal absorption. Laurocapram can also increase the adhesion to the skin surface, so that the functional substance stays on the skin for a longer time, thereby improving the absorption effect;

[0028] 5. The aloe polysaccharide added to the product of the present invention is a natural plant extract from aloe. When the mucus formed by aloe polysaccharide in water is placed on the wound, the wound remains moist and is not easy to fall off. At the same time, the epidermal and fibroblast growth factors from the mucus directly stimulate the growth and repair of epidermal cells to increase wound healing. At the same time, aloe polysaccharide also has pharmacological effects such as promoting wound healing and tissue repair, reducing scar formation, protecting mucosa, breathable and moisturizing, antibacterial, and anti-inflammatory;

[0029] 6. The zinc hyaluronate added to the product of the present invention is a zinc salt of hyaluronic acid, which is prepared by ion exchange between zinc ions and sodium ions on the carboxyl group of sodium hyaluronate. It is a combination of hyaluronic acid and zinc. It not only has the super hydrating, moisturizing, water-locking, repairing and nourishing properties of hyaluronic acid, but also has the soothing effects of zinc, accelerating the growth and division of epidermal cells, promoting wound healing and inhibiting bacteria;

[0030] 7. The bird's nest acid added to the product of the present invention can promote the synthesis of skin cell molecules and cell metabolism, and has the function of soothing the skin and repairing the skin barrier of pets. It can also provide deep nourishment and protection, and help improve the skin health of pets. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] The present invention will be further described below in conjunction with the accompanying drawings and embodiments:

[0032] Figure 1 It is a comparison result diagram of viscosity scores of natural skin care hydrogels for pet wounds at room temperature in various embodiments and comparative examples;

[0033] Figure 2 The natural skin care hydrogel system for pet wounds in each embodiment and comparative example is shown in 0.1~200 s -1 Shear stress changes within a certain range;

[0034] Figure 3 The natural skin care hydrogel system for pet wounds in each embodiment and comparative example is shown in 0.1~200 s -1 Apparent viscosity changes within the range;

[0035] Figure 4 It is a comparison result diagram of the water absorption of the natural skin care hydrogel for pet wounds in each embodiment and comparative example;

[0036] Figure 5 This is a comparison chart of the weight loss rates of the natural skin care hydrogels for pet wounds in various embodiments and comparative examples;

[0037] Figure 6 This is a comparison chart of the air permeability of the natural skin care hydrogel for pet wounds in each embodiment and comparative example;

[0038] Figure 7 The skin condition of the experimental cat 3 hours after applying the pet wound natural skin care hydrogel of Example 1 is shown. DETAILED DESCRIPTION

[0039] The implementation of the present invention is not limited to the following embodiments, and any form of modification and / or change made to the present invention will fall within the scope of protection of the present invention. In the present invention, unless otherwise specified, all equipment and raw materials can be purchased from the market or are commonly used in the industry. Unless otherwise specified, the methods used in the embodiments are common techniques in the art.

[0040] For numerical ranges, the endpoints of each range, the endpoints of each range and the individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be deemed to be specifically disclosed herein.

[0041] The main reagents and test instruments used in the following examples are shown in Table 1.

[0042] Example 1

[0043] This embodiment provides a natural skin care hydrogel for pet wounds, and the preparation method is as follows:

[0044] (1) Weigh 0.5 parts of aloe polysaccharide in a proper amount of deionized water, stir with a magnetic stirrer at 500 rpm / min at 40°C for 10 min until completely dissolved, cool to room temperature to obtain aloe polysaccharide skin care liquid, and seal in a volumetric bottle for later use;

[0045] (2) Weigh 3 parts of zinc hyaluronate in a certain proportion and add them to an appropriate amount of deionized water. Stir the mixture with a magnetic stirrer at 1000 rpm / min at 50°C for 15 min until the mixture is completely dissolved. Cool the mixture to room temperature to obtain zinc hyaluronate skin care liquid. Seal the liquid in a volumetric flask for later use.

[0046] (3) Weigh 0.5 parts of gel-forming agent according to the proportion; add the gel-forming agent into deionized water, stir it with a magnetic force at a speed of 1500 r / min for 1 hour, and then soak it at room temperature for 10 hours to allow it to fully hydrate and swell, thereby obtaining liquid A;

[0047] (4) Take another 0.25 parts of triethanolamine, 0.5 parts of water-soluble lauryl azone, 0.1 parts of mint essence, and 0.05 parts of ethylparaben, dissolve them in deionized water and sonicate for 30 minutes to completely dissolve them to obtain Solution B;

[0048] (5) Weigh 0.1 parts of sialic acid, 0.2 parts of zinc gluconate, 0.2 parts of vitamin E, 0.1 parts of vitamin B, 0.5 parts of PEG-40 castor oil and 0.5 parts of polyethylene glycol in proportion, add them into deionized water, stir them with magnetic force at 2000 rpm / min at 70°C for 1 h to completely dissolve them, and obtain liquid C;

[0049] (6) Add 10 parts of moisturizer to liquid A and grind evenly, then slowly add to liquid B and stir and mix continuously for 1 hour until the hydrogel matrix is ​​formed, then add liquid C, aloe polysaccharide skin care lotion and hyaluronic acid zinc skin care lotion in sequence, stir and mix evenly, then adjust the pH to 6.0-8.0 with 0.1 mol / L NaOH solution to obtain the natural skin care hydrogel for pet wounds.

[0050] Furthermore, the ratio of the gel forming agent to triethanolamine used in the above steps is 1:0.5.

[0051] Furthermore, the gel forming agent is Carbomer 934.

[0052] Furthermore, the moisturizing agent is a mixture of glycerol and propylene glycol, and the mass ratio of the two is 1:1.

[0053] Example 2

[0054] This embodiment provides a natural skin care hydrogel for pet wounds, and the preparation method is as follows:

[0055] (1) Weigh 1 portion of aloe polysaccharide in a proper amount of deionized water, stir with a magnetic stirrer at 500 rpm / min at 45°C for 10 min until completely dissolved, cool to room temperature to obtain aloe polysaccharide skin care liquid, and seal in a volumetric bottle for later use;

[0056] (2) Weigh 2 parts of zinc hyaluronate in a proper amount of deionized water, stir with a magnetic stirrer at 1000 rpm / min at 50°C for 15 min until completely dissolved, cool to room temperature to obtain zinc hyaluronate skin care liquid, and seal in a volumetric flask for later use;

[0057] (3) Weigh 1.5 parts of gel-forming agent according to the proportion; add the gel-forming agent into deionized water, stir it with a magnetic stirrer at a speed of 1500 r / min for 2 h, and then soak it at room temperature for 12 h to allow it to fully hydrate and swell, thereby obtaining liquid A;

[0058] (4) Take another 1.5 parts of triethanolamine, 1.0 parts of water-soluble lauryl azone, 0.2 parts of mint essence, and 0.07 parts of ethylparaben, dissolve them in deionized water by ultrasonication for 45 minutes to completely dissolve them, and obtain liquid B;

[0059] (5) Weigh 0.2 parts of sialic acid, 0.3 parts of zinc gluconate, 0.5 parts of vitamin E, 0.2 parts of vitamin B, 1 part of PEG-40 castor oil and 1.5 parts of polyethylene glycol in proportion, add them into deionized water, stir magnetically at 2000 rpm / min at 70°C for 2.5 h to completely dissolve them, and obtain liquid C;

[0060] (6) Add 15 parts of moisturizer to liquid A and grind evenly, then slowly add to liquid B and stir and mix continuously for 2 hours until the hydrogel matrix is ​​formed, then add liquid C, aloe polysaccharide skin care lotion and hyaluronic acid zinc skin care lotion in sequence, stir and mix evenly, then adjust the pH to 6.0-8.0 with 0.1 mol / L NaOH solution to obtain the natural skin care hydrogel for pet wounds.

[0061] Furthermore, in the above steps, the ratio of the gel forming agent to triethanolamine is 1:1.

[0062] Furthermore, the gel-forming agent is a mixture of carbomer 934 and hydroxypropyl methylcellulose, and the mass ratio of the two is 1:1.

[0063] Furthermore, the moisturizing agent is a mixture of glycerin, propylene glycol and collagen, and the mass ratio of the three is 1:1:1.

[0064] Example 3

[0065] This embodiment provides a natural skin care hydrogel for pet wounds, and the preparation method is as follows:

[0066] (1) Weigh 1.5 parts of aloe polysaccharide in a proper amount of deionized water, stir with a magnetic stirrer at 500 rpm / min at 45°C for 10 min until completely dissolved, cool to room temperature to obtain aloe polysaccharide skin care liquid, and seal in a volumetric bottle for later use;

[0067] (2) Weigh 0.5 parts of zinc hyaluronate in a proper amount of deionized water, stir with a magnetic stirrer at 1000 rpm / min at 50°C for 15 min until completely dissolved, cool to room temperature to obtain zinc hyaluronate skin care liquid, and seal in a volumetric flask for later use;

[0068] (3) Weigh 3 parts of gel-forming agent according to the proportion; add the gel-forming agent to deionized water, stir it with a magnetic stirrer at a speed of 1500 r / min for 2.5 h, and then soak it at room temperature for 13 h to allow it to fully hydrate and swell, thereby obtaining liquid A;

[0069] (4) Dissolve 4.5 parts of triethanolamine, 1.5 parts of water-soluble lauryl azone, 0.3 parts of mint essence, and 0.08 parts of ethylparaben in deionized water and sonicate for 50 minutes to completely dissolve them to obtain Solution B;

[0070] (5) Weigh 0.4 parts of sialic acid, 0.5 parts of zinc gluconate, 0.8 parts of vitamin E, 0.5 parts of vitamin B, 2 parts of PEG-40 castor oil and 2 parts of polyethylene glycol in proportion, add them into deionized water, stir them with magnetic force at 2000 rpm / min at 70°C for 3 h to completely dissolve them, and obtain liquid C;

[0071] (6) Add 20 parts of moisturizer to liquid A and grind evenly, then slowly add to liquid B and stir and mix continuously for 3 hours until the hydrogel matrix is ​​formed, then add liquid C, aloe polysaccharide skin care lotion and hyaluronic acid zinc skin care lotion in sequence, stir and mix evenly, then adjust the pH to 6.0-8.0 with 0.1 mol / L NaOH solution to obtain the natural skin care hydrogel for pet wounds.

[0072] Furthermore, in the above steps, the ratio of the gel forming agent to triethanolamine is 1:1.5.

[0073] Furthermore, the gel-forming agent is a mixture of carbomer 934, hydroxypropyl methylcellulose and hydroxypropyl cellulose, and the mass ratio of the three is 1:1:1.

[0074] Furthermore, the moisturizing agent is a mixture of glycerin, propylene glycol and sorbitol, and the mass ratio of the three is 1:1:1.

[0075] Example 4

[0076] This embodiment provides a natural skin care hydrogel for pet wounds, and the preparation method is as follows:

[0077] (1) Weigh 0.6 parts of aloe polysaccharide in a proper amount of deionized water, stir with a magnetic stirrer at 500 rpm / min at 40°C for 10 min until completely dissolved, cool to room temperature to obtain aloe polysaccharide skin care liquid, and seal in a volumetric bottle for later use;

[0078] (2) Weigh 4 parts of zinc hyaluronate in a certain proportion and add it to an appropriate amount of deionized water. Stir the mixture with a magnetic stirrer at 1000 rpm / min at 50°C for 15 min until it is completely dissolved. Cool the mixture to room temperature to obtain zinc hyaluronate skin care liquid. Seal the solution in a volumetric flask for later use.

[0079] (3) Weigh 0.6 parts of gel-forming agent according to the proportion; add the gel-forming agent into deionized water, stir it with a magnetic force at a speed of 1500 r / min for 1 hour, and then soak it at room temperature for 11 hours to allow it to fully hydrate and swell, thereby obtaining liquid A;

[0080] (4) Take another 0.3 part of triethanolamine, 0.5 part of water-soluble lauryl azone, 0.1 part of mint essence, and 0.05 part of ethylparaben, dissolve them in deionized water and sonicate for 30 minutes to completely dissolve them to obtain Solution B;

[0081] (5) Weigh 0.15 parts of sialic acid, 0.2 parts of zinc gluconate, 0.2 parts of vitamin E, 0.1 parts of vitamin B, 0.5 parts of PEG-40 castor oil and 0.5 parts of polyethylene glycol in proportion, add them into deionized water, stir magnetically at 2000 rpm / min at 70°C for 1 h to completely dissolve them, and obtain liquid C;

[0082] (6) Add 10 parts of moisturizer to liquid A and grind evenly, then slowly add to liquid B and stir and mix continuously for 1 hour until the hydrogel matrix is ​​formed, then add liquid C, aloe polysaccharide skin care lotion and hyaluronic acid zinc skin care lotion in sequence, stir and mix evenly, then adjust the pH to 6.0-8.0 with 0.1 mol / L NaOH solution to obtain the natural skin care hydrogel for pet wounds.

[0083] Furthermore, in the above steps, the ratio of the gel forming agent to triethanolamine is 1:0.5.

[0084] Furthermore, the gel forming agent is Carbomer 940.

[0085] Furthermore, the moisturizing agent is a mixture of glycerol and propylene glycol, and the mass ratio of the two is 1:1.

[0086] Example 5

[0087] This embodiment provides a natural skin care hydrogel for pet wounds, and the preparation method is as follows:

[0088] (1) Weigh 1.2 parts of aloe polysaccharide in a proper amount of deionized water, stir with a magnetic stirrer at 500 rpm / min at 45°C for 10 min until completely dissolved, cool to room temperature to obtain aloe polysaccharide skin care liquid, and seal in a volumetric bottle for later use;

[0089] (2) Weigh 2 parts of zinc hyaluronate in a proper amount of deionized water, stir with a magnetic stirrer at 1000 rpm / min at 50°C for 15 min until completely dissolved, cool to room temperature to obtain zinc hyaluronate skin care liquid, and seal in a volumetric flask for later use;

[0090] (3) Weigh 1.2 parts of gel forming agent according to the proportion; add the gel forming agent into deionized water, stir it with magnetic force at 1500 r / min for 2 h, and then soak it at room temperature for 13 h to allow it to fully hydrate and swell, thereby obtaining liquid A;

[0091] (4) Dissolve 1.2 parts of triethanolamine, 1.0 parts of water-soluble lauryl azone, 0.2 parts of mint essence, and 0.07 parts of ethylparaben in deionized water and sonicate for 45 minutes to completely dissolve them to obtain Solution B;

[0092] (5) Weigh 0.3 parts of sialic acid, 0.3 parts of zinc gluconate, 0.5 parts of vitamin E, 0.2 parts of vitamin B, 1 part of PEG-40 castor oil and 1.5 parts of polyethylene glycol in proportion, add them into deionized water, stir them with magnetic force at 2000 rpm / min at 70°C for 2.5 h to completely dissolve them, and obtain liquid C;

[0093] (6) Add 15 parts of moisturizer to liquid A and grind evenly, then slowly add to liquid B and stir and mix continuously for 2 hours until the hydrogel matrix is ​​formed, then add liquid C, aloe polysaccharide skin care lotion and hyaluronic acid zinc skin care lotion in sequence, stir and mix evenly, then adjust the pH to 6.0-8.0 with 0.1 mol / L NaOH solution to obtain the natural skin care hydrogel for pet wounds.

[0094] Furthermore, in the above steps, the ratio of the gel forming agent to triethanolamine is 1:1.

[0095] Furthermore, the gel-forming agent is a mixture of carbomer 940 and hydroxypropyl methylcellulose, and the mass ratio of the two is 1:1.

[0096] Furthermore, the moisturizing agent is a mixture of glycerin, propylene glycol and collagen, and the mass ratio of the three is 1:1:1.

[0097] Example 6

[0098] This embodiment provides a natural skin care hydrogel for pet wounds, and the preparation method is as follows:

[0099] (1) Weigh 1.8 parts of aloe polysaccharide in a proper amount of deionized water, stir with a magnetic stirrer at 500 rpm / min at 45°C for 10 min until completely dissolved, cool to room temperature to obtain aloe polysaccharide skin care liquid, and seal in a volumetric bottle for later use;

[0100] (2) Weigh 1 part of zinc hyaluronate in a proper amount of deionized water, stir with a magnetic stirrer at 1000 rpm / min at 50°C for 15 min until completely dissolved, cool to room temperature to obtain zinc hyaluronate skin care liquid, and seal in a volumetric flask for later use;

[0101] (3) Weigh 2.4 parts of gel forming agent according to the proportion; add the gel forming agent into deionized water, stir magnetically at a speed of 1500 r / min for 2.5 h, and then soak at room temperature for 14 h to allow it to fully hydrate and swell, thereby obtaining liquid A;

[0102] (4) Take another 3.6 parts of triethanolamine, 1.5 parts of water-soluble lauryl azone, 0.3 parts of mint essence, and 0.08 parts of ethylparaben, dissolve them in deionized water and sonicate for 50 minutes to completely dissolve them to obtain Solution B;

[0103] (5) Weigh 0.6 parts of sialic acid, 0.5 parts of zinc gluconate, 0.8 parts of vitamin E, 0.5 parts of vitamin B, 2 parts of PEG-40 castor oil and 2 parts of polyethylene glycol in proportion, add them into deionized water, stir them with magnetic force at 2000 rpm / min at 70°C for 3 h to completely dissolve them, and obtain liquid C;

[0104] (6) Add 20 parts of moisturizer to liquid A and grind evenly, then slowly add to liquid B and stir and mix continuously for 3 hours until the hydrogel matrix is ​​formed, then add liquid C, aloe polysaccharide skin care lotion and hyaluronic acid zinc skin care lotion in sequence, stir and mix evenly, then adjust the pH to 6.0-8.0 with 0.1 mol / L NaOH solution to obtain the natural skin care hydrogel for pet wounds.

[0105] Furthermore, in the above steps, the ratio of the gel forming agent to triethanolamine is 1:1.5.

[0106] Furthermore, the gel-forming agent is a mixture of carbomer 940, hydroxypropyl methylcellulose and hydroxypropyl cellulose, and the mass ratio of the three is 1:1:1.

[0107] Furthermore, the moisturizing agent is a mixture of glycerin, propylene glycol and sorbitol, and the mass ratio of the three is 1:1:1.

[0108] Example 7

[0109] Example 7 is different from Example 3 only in that the gel forming agent is a mixture of chitosan hydrochloride and hydroxypropyl cellulose, and the mass ratio of the two is 1:1.

[0110] Example 8

[0111] Example 8 is different from Example 2 only in that the gel forming agent is a mixture of chitosan hydrochloride, hydroxypropyl methylcellulose and hydroxypropyl cellulose, and the mass ratio of the three is 1:1:1.

[0112] Comparative Example 1

[0113] Comparative Example 1 is different from Example 1 in that water-soluble laurocapram is used in place of zinc hyaluronate; and the gel-forming agent is sodium alginate.

[0114] Comparative Example 2

[0115] Comparative Example 2 is different from Example 2 in that water-soluble laurocapram is used to replace aloe polysaccharide; the gel-forming agent is a mixture of sodium alginate and hydroxypropyl methylcellulose, and the mass ratio of the two is 1:1.

[0116] Comparative Example 3

[0117] Comparative Example 3 is different from Example 3 in that deionized water of equal mass is used to replace zinc hyaluronate and aloe polysaccharide; the gel-forming agent is a mixture of sodium alginate, hydroxypropyl methylcellulose and hydroxypropyl methylcellulose, and the mass ratio of the three is 1:1:1.

[0118] Comparative Example 4

[0119] Comparative Example 4 is different from Example 4 in that water-soluble laurocapram is used in place of zinc hyaluronate in terms of mass; and the gel-forming agent is sodium carboxymethyl cellulose.

[0120] Comparative Example 5

[0121] Comparative Example 5 is different from Example 5 in that aloe polysaccharide of equal mass is used to replace zinc hyaluronate; the gel-forming agent is a mixture of sodium carboxymethyl cellulose and hydroxypropyl methylcellulose, and the mass ratio of the two is 1:1.

[0122] Test Example 1 - Investigation of the properties of natural skin care hydrogel for pet wounds

[0123] This test example examines the properties of the natural pet wound skin care hydrogels described in the above embodiments and comparative examples, specifically including appearance, pH value, formability, uniformity and spreadability.

[0124] Appearance: The prepared pet wound natural skin care hydrogel was observed for appearance.

[0125] pH value: Take 10 g of the pet wound natural skin care hydrogel sample and place it in a beaker, add 100 mL of water, stir well and measure its pH value.

[0126] Formability: The formability of the prepared natural pet wound skin care hydrogel was observed.

[0127] Homogeneity: Place 3 g of the natural pet wound skin care hydrogel in a 5 mL centrifuge tube and centrifuge at 3 000 r / min for 30 min to see if there are layers.

[0128] Spreadability: Apply the pet wound natural skin care hydrogel evenly on the skin and observe whether it can be spread evenly.

[0129] The results of the investigation on the properties of the natural skin care hydrogels for pet wounds described in the embodiments and comparative examples are shown in Table 2.

[0130] As can be seen from Table 2, the properties of the natural skin care hydrogels for pet wounds prepared in various embodiments of the present invention are better than those of the comparative examples. The natural skin care hydrogels for pet wounds prepared in Examples 1-8 all have a transparent gel-like appearance, and are all formed without stratification. The gel is uniform, stable, easy to spread, and has good ductility; while Comparative Example 3 is translucent gel-like and not easy to spread; the skin care hydrogels prepared in Comparative Examples 1 and 4 are not formed, and the skin care hydrogels prepared in Comparative Examples 2 and 5 are in a semi-formed state, and none of them meet the gel forming standard, and gel formation is difficult. At the same time, the pH of the natural skin care hydrogels for pet wounds prepared in various embodiments of the present invention is between 6.5-7.5, which is close to neutral, meets the requirement that the pH of the topical preparation is not greater than 8.3, is relatively gentle and non-irritating to the pet skin, while the pH of Comparative Examples 4 and 5 is greater than 8.5, which does not meet the requirement that the pH of the topical preparation is not less than 8.3.

[0131] Test Example 2 - Functional Investigation of Natural Skin Care Hydrogel for Pet Wounds

[0132] This test example examines the functionality of the natural pet wound skin care hydrogel of the present invention from multiple perspectives, including apparent viscosity, water absorption, and moisturizing and breathability.

[0133] Viscosity score at room temperature: after tilting 45°, it needs to be shaken vigorously to flow, the viscosity is excellent (4 points); after tilting 45°, it flows with a slight shake, the viscosity is good (3 points); after tilting 45°, it flows slowly within 1 to 3 seconds, the viscosity is medium (2 points); after tilting 45°, it flows quickly, the viscosity is poor (1 point). (For specific methods, please refer to: Xi Wang, Yang Liu, Wang Yunshan, et al. Preparation of sodium alginate / chitosan earthworm protein gel and its effect on promoting the healing of deep second-degree burns in rats [J]. Chinese Medicinal Materials, 2011, 34(10): 1583-1588.).

[0134] Depend on Figure 1 It can be seen that the viscosity scores of the natural skin care hydrogels for pet wounds prepared in various embodiments of the present invention are all between 3 and 4 points, which are excellent or good viscosity states, while the viscosity of Comparative Examples 2 and 5 is medium, with a viscosity score of 2 points; the viscosity of Comparative Examples 1 and 4 is poor, with a viscosity score of 1 point. This indicates that the viscosity state of the natural skin care hydrogels for pet wounds prepared in various embodiments of the present invention is significantly better than that of the comparative examples.

[0135] Rheological investigation: The static rheological analysis of the natural skin care hydrogels for pet wounds prepared in the above-mentioned embodiments and comparative examples was performed using a rotational rheometer. An appropriate amount of the natural skin care hydrogel samples for pet wounds were taken from each group, and the samples were placed on a rheometer plate with a diameter of 40 mm, and the plate spacing was set to 1.0 mm. The excess sample was removed from the plate with a scraper, and silicone oil was applied to the surface to prevent water from volatilizing. The test temperature was set to 25°C, the gap was 1.0 mm, and the shear rate was 0.1-200 s. -1 , measure the shear stress and apparent viscosity at different shear rates. Each group of samples is tested three times. A curve graph is drawn based on the test data and analyzed.

[0136] In static rheological analysis, Figure 2 and Figure 3 It can be seen that the natural skin care hydrogel for pet wounds prepared in each embodiment of the present invention has a higher shear stress and apparent viscosity than the hydrogel prepared in the comparative example at the same shear rate. The gel effect prepared in the embodiment makes the hydrogel have a higher shear stress and apparent viscosity, and the gel network mechanical properties and density formed in the corresponding hydrogel are stronger, and a denser cross-linked structure is formed inside the gel, which enhances the mechanical strength of the hydrogel and is conducive to promoting the stability of its structure. It can be seen that the static rheological properties of the product of the present invention are significantly better than those of the comparative example, indicating that the gel forming agent, humectant, aloe polysaccharide, zinc hyaluronate and laurocapram added to the product of the present invention can improve the gel network mechanical properties, density and structural stability of the hydrogel.

[0137] Water absorption determination: 10 g of natural skin care hydrogel sample for pet wounds of each group was placed in a test tube with an inner diameter of 1.8 cm. The sample was centrifuged at 1000 r / min for 5 min and then weighed. 5 mL of water was added and the water layer was carefully poured off after 12 h. The mass was weighed and the water absorption was calculated.

[0138] Depend on Figure 4 It can be seen that the water absorption of the natural skin care hydrogel for pet wounds prepared in each embodiment of the present invention is higher than 20%, and the water absorption can reach up to about 40%, while the water absorption of the hydrogel in the comparative example is lower than 20%. It can be seen that the water absorption performance of the product of the present invention is significantly better than that of the comparative example, indicating that the gel forming agent, humectant, aloe polysaccharide, zinc hyaluronate and laurocapram added to the product of the present invention can significantly improve the water absorption performance of the hydrogel, which is beneficial to absorb excess exudate from the wound tissue and keep the wound clean.

[0139] Moisture Retention: At 10 cm 2 The pet wound natural skin care hydrogels of each group were spread on the flat dish, and the weight was weighed and placed under ventilation at 40℃. The weight loss rate was measured after 48 hours.

[0140] Depend on Figure 5 It can be seen that the weight loss rates of the natural skin care hydrogels for pet wounds prepared in the embodiments of the present invention are all lower than 12.5%, and the lowest weight loss rate can reach about 7.5%, while the weight loss rates of the hydrogels in the comparative examples are all higher than 14.5%. The higher the weight loss rate of the hydrogel, the lower the moisture retention. It can be seen that the moisture retention performance of the product of the present invention is significantly better than that of the comparative example, indicating that the gel forming agent, moisturizing agent, aloe polysaccharide, zinc hyaluronate and laurocapram added to the product of the present invention can significantly improve the moisture retention performance of the hydrogel, can keep the wound surface moist and have the dual effects of hydration and absorption, and autonomously moisturize the wound surface to promote granulation growth and epithelial regeneration.

[0141] Air permeability measurement: Add more than 90% of the volume of water into the glass bottle, cover the mouth of the bottle with the natural skin care hydrogel for pet wounds of each group, and weigh the mass at this time and record it as Wi Then place it at 37˚C for 24 h and then take it out and weigh the remaining mass. Wt , calculate the hydrogel permeability.

[0142] Breathability: WVTR = Wi-Wt / A × t (g / m 2 / day)

[0143] In the formula, A is the bottle mouth area; t is the reaction time; Wi is the initial mass; Wt is the mass after reaction.

[0144] Depend on Figure 6 It can be seen that the air permeability of the natural skin care hydrogel for pet wounds prepared in each embodiment of the present invention is higher than 4300 g / m 2 / day, and the air permeability can reach up to 6500 g / m 2 / day, while the air permeability of the comparative hydrogels was lower than 4000g / m 2 / day, and the minimum air permeability is 2000 g / m 2 / day, and the ideal air permeability of hydrogel dressing should be greater than 3000 g / m 2 / day to maintain sufficient gas exchange with the outside world. It can be seen that the air permeability of the product of the present invention is significantly better than that of the comparative example, indicating that the gel forming agent, moisturizing agent, aloe polysaccharide, zinc hyaluronate and laurocapron added to the product of the present invention can significantly improve the air permeability of the hydrogel, indicating that the hydrogel can provide sufficient gas exchange for the wound during the recovery period and provide good repair conditions for skin trauma repair.

[0145] Heat resistance experiment: The same batch of natural skin care hydrogels for pet wounds in various groups were placed in a sealed clean container at 60°C for 10 days. Samples were taken on the 5th and 10th days, and their appearance was observed and compared with the samples on the 0th day.

[0146] Cold resistance experiment: The same batch of natural skin care hydrogels for pet wounds in various groups were placed in a sealed clean container at -15°C for 10 days. Samples were taken on the 5th and 10th days, and their appearance was observed and compared with the samples on the 0th day.

[0147] As can be seen from Table 3, the results of the heat and cold stability test of the natural skin care hydrogel for pet wounds prepared in each embodiment of the present invention are better than those of the comparative example. The skin care hydrogel prepared in Examples 1-8 was placed at 60°C for 5 days and 10 days, and there was no significant difference in appearance after returning to room temperature and before the test, and the heat resistance was good; at the same time, it was placed at -15°C for 5 days and 10 days, and there was no significant difference in appearance after returning to room temperature and before the test, and the cold resistance was good. The comparative example changed its appearance under high temperature 60°C and low temperature -15°C, and its heat and cold stability was poor.

[0148] Animal test: The pet wound natural skin care hydrogel prepared in Example 1 was subjected to a pet acute skin irritation and sensitivity test. Before the test, the hair on both sides of the spine and the soles of the feet of the experimental cats was trimmed, and the hair removal range was about 2cm×3cm respectively. The skin care gel was applied evenly on the skin surface. The skin reaction of the experimental cats at the application site was observed 1h, 3h and 6h after the application of the skin care gel. The test results are shown in Table 4 and Figure 7 shown.

[0149] From Table 4 and Figure 7 It can be seen that the natural skin care hydrogel for pet wounds prepared in Example 1 of the present invention is non-irritating and non-sensitizing to the skin of pet cats, and the skin condition does not change before and after application, indicating that the natural skin care hydrogel for pet wounds prepared by the present invention is mild and non-irritating, highly safe, and suitable for long-term use by pets.

[0150] Finally, it should be noted that the above is only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred arrangement scheme, a person skilled in the art should understand that the technical solution of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solution of the present invention.

Claims

1. A natural skin care hydrogel for pet wounds, characterized in that: The hydrogel comprises the following raw materials in percentage by weight: Gel former 0.5-5%, moisturizer 10-25%, aloe polysaccharide 0.1-2%, zinc hyaluronate 0.5-4%, swiftlet acid 0.1-1%, lauryl azone 0.2-2%, zinc gluconate 0.1-0.5%, vitamin E 0.2-1%, vitamin B 0.1-1%, PEG-40 castor oil 0.5-3%, polyethylene glycol 0.5-5%, triethanolamine 0.25-5%, mint flavor 0.1-1%, ethylparaben 0.05-0.1%, the balance is deionized water, and the sum of the mass percentages of the raw material components is 100%; The ratio of the gel forming agent to triethanolamine is 1:0.5-2; The gel forming agent is one or a combination of two or more of carbomer 934, carbomer 940, sodium carboxymethyl cellulose, sodium alginate, chitosan hydrochloride, hydroxypropyl cellulose and hydroxypropyl methyl cellulose.

2. The natural skin care hydrogel for pet wounds according to claim 1, characterized in that: The moisturizing agent is one or a combination of two or more of glycerol, propylene glycol, collagen and sorbitol.

3. The natural skin care hydrogel for pet wounds according to claim 1, characterized in that: The purity of the aloe polysaccharide is not less than 60%.

4. The pet wound natural skin care hydrogel according to claim 1, characterized in that: The zinc hyaluronate is hydrolyzed zinc hyaluronate, and its molecular weight is 100-500 kDa.

5. The natural skin care hydrogel for pet wounds according to claim 1, characterized in that: The lauryl azone is water-soluble lauryl azone, and its purity is not less than 98%.

6. A method for preparing the natural skin care hydrogel for pet wounds as claimed in any one of claims 1 to 5, characterized in that: The preparation method comprises the following steps: (1) Weigh aloe polysaccharide in a certain amount of deionized water, stir under magnetic stirring at 40-50°C until completely dissolved, cool to room temperature to obtain aloe polysaccharide skin care liquid, and seal in a volumetric bottle for later use; (2) Weigh zinc hyaluronate in a certain amount of deionized water, stir under magnetic stirring at 40-50°C until completely dissolved, cool to room temperature to obtain zinc hyaluronate skin care liquid, and seal in a volumetric flask for later use; (3) Weigh a gel forming agent according to a certain proportion, add the gel forming agent into deionized water, stir magnetically for 1-3 hours, and then soak at room temperature for 10-14 hours to allow the gel forming agent to fully hydrate and swell, thereby obtaining liquid A; (4) Weigh triethanolamine, laurocapram, mint essence, and ethylparaben in proportion, add them to deionized water, and sonicate at 50-60°C for 30-60 min to completely dissolve them to obtain solution B; (5) Weigh sialic acid, zinc gluconate, vitamin E, vitamin B, PEG-40 castor oil and polyethylene glycol in proportion, add them to deionized water, stir magnetically at 60-70°C for 1-4 hours to completely dissolve them, and obtain liquid C; (6) After adding moisturizer to liquid A and grinding evenly, slowly add it to liquid B, and stir and mix continuously for 1-3 hours until the hydrogel matrix is ​​formed. Then add liquid C, aloe polysaccharide skin care lotion and hyaluronic acid zinc skin care lotion in sequence, stir and mix evenly, and adjust the pH to 6.0-8.0 with NaOH solution to obtain the natural skin care hydrogel for pet wounds.

7. Use of the natural pet wound skin care hydrogel according to any one of claims 1 to 5 in the preparation of pet wound repair products.

8. The use according to claim 7, characterized in that: The pets include pet cats, pet dogs, pet rabbits and pet mice, and the products are wound repair care products or medicines.

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