Intensive farmland corn pest green prevention and control method

Abstract

The invention provides an intensive farmland corn pest green prevention and control method, and belongs to the technical field of green prevention and control. The invention provides an intensive green prevention and control method for farmland corn pests. The method is based on a scheme of combining at least half use of high-efficiency low-toxicity pesticides and prevention and control technologies of artificial flower and grass belts. According to the method, a synergistic effect can be generated, the sensitivity of the corn borers to natural enemies is improved, the population of the natural enemies is protected, the harm of the corn borers is synergistically prevented and controlled, the use of insecticides is reduced, and biodiversity protection and green production of crops are realized.

Description

Technical Field

[0001] The invention belongs to the technical field of green pest control, and in particular relates to a green pest control method for intensive farmland corn pests. Background Art

[0002] Corn borers are extremely harmful to corn. The damage rate of spring corn is about 30%, resulting in a 10% reduction in yield. The damage rate of summer corn can reach 90%, generally resulting in a 20% to 30% reduction in yield. Intensification is the mainstream direction of agriculture, but large-scale planting has also led to the reduction of agricultural biodiversity and the imbalance of ecological functions. In intensive corn planting, the damage caused by corn borers is very serious, with a general reduction of 15% to 30%. At the same time, it induces fungal toxin pollution, and both yield and quality face production bottlenecks.

[0003] Chemical pesticides are currently mostly used to control corn pests. However, due to the problem of pest resistance, the use of chemical pesticides is increasing, causing serious impacts on the environment. At the same time, they have a toxic effect on natural enemies. Therefore, it is necessary to explore green control methods. Summary of the invention

[0004] The present invention provides an intensive green control method for corn pests in farmland, which increases the sensitivity of corn pests to natural enemies, protects natural enemy populations, coordinates prevention and control, reduces the use of pesticides, and achieves biodiversity protection and green production of crops.

[0005] The present invention provides a method for green control of corn pests in intensive farmland, based on a scheme combining the use of at least half of high-efficiency and low-toxic pesticides and the control technology of artificial flower and grass belts, firstly, screening high-efficiency and low-toxic corn borer insecticide varieties, taking half the amount or less application, producing sublethal toxicity to corn pests, thereby being more sensitive to natural enemies, and reducing the amount of pesticides to reduce the damage to natural enemy parasitic wasps; secondly, constructing an artificial flower and grass belt containing a combination of nectar plants at the edge of a corn field, the nectar plants in the flower and grass belt can provide nectar, pollen and alternative prey for natural enemies, not only prolonging the life of parasitic wasps, increasing the reproductive capacity of natural enemies, and the nutrients in pollen can increase the detoxification ability of natural enemies to pesticides. The method of the present invention can produce a synergistic effect, increase the sensitivity of corn borers to natural enemies, protect natural enemy populations, coordinately control corn borer damage, reduce the use of pesticides, reduce grain pesticide pollution and fungal toxin pollution, and achieve biodiversity protection and green production of crops. DETAILED DESCRIPTION

[0006] The present invention provides a method for intensive green control of corn pests in farmland, comprising the following steps:

[0007] (1) Select insecticide varieties that are highly effective and low in toxicity against major corn pests and reduce the application rate by at least half;

[0008] (2) constructing an artificial flower and grass belt beside a corn field, wherein the artificial flower and grass belt contains nectar plants that are natural enemies of major corn pests.

[0009] The main corn pests of the present invention include lepidopteran pests such as corn borers. The present invention first screens out high-efficiency and low-toxic insecticides from commercial corn borer insecticides for use in the method of the present invention; specifically, screening corn borers by indoor toxicity determination, and the determination standard can be "Pesticide Indoor Bioassay Test Guidelines for Pesticides Part 10: Artificial Feed Mixing Method" (NY / T1154.10-2008). In addition to screening the killing effect on corn borers, the present invention also needs to use the natural enemy of corn borers, trichogrammatids, for screening, so that the insecticides with lower indoor toxicity to trichogrammatids can be applied to the present invention.

[0010] The insecticide varieties used for screening of the present invention include at least one of the following: avermectin benzoate, chlorantraniliprole, tetrachlorantraniliprole, azadirachtin, chlorfenapyr, methoxyfenozide and phoxim. After comprehensive screening, the high-efficiency and low-toxic insecticide variety screened out is chlorantraniliprole, and its indoor toxicity LC to corn borer larvae is 50 The (95% CL) value is 0.50 mg / L, and the indoor toxicity LC to Trichogramma ostriniae 50 The (95% CL) value was 177.9 mg / L.

[0011] The high-efficiency and low-toxic insecticide variety screened out in the present invention is chlorantraniliprole. When corn borer holes are first seen in the field during the corn tasseling period, 10 mL of 200 g / L chlorantraniliprole suspension is diluted with 50 L of water per mu and sprayed evenly, and the heart leaves are irrigated once.

[0012] The artificial flower and grass belt in step (2) of the present invention is constructed at the edge of the farmland, and the width of the artificial flower and grass belt is 2 to 4 meters. In an embodiment of the present invention, when the field is square, the plant buffer belt is constructed along the long side of the field. If the field is irregular in shape, the plant buffer belt is constructed along the periphery of the field. Before planting, the artificial flower and grass belt of the present invention preferably uses compound fertilizer as base fertilizer, sowing perennial nectar plants in the autumn of the first year; sowing annual nectar plants in the spring of the second year. For example, in one embodiment of the present invention, 40 kilograms of compound fertilizer (N: P: K = 15: 15: 15) is applied per mu as base fertilizer before planting; when sowing in the autumn of the first year, the perennial plant mixed grass seeds are sown by a hand-cranked seeder, and the weight of the sown grass seeds per square meter is 5 grams. After sowing, the soil is covered with 1 cm, and the soil is compacted with a compactor. When sowing in the spring of the second year, the annual mixed grass seeds are sown in rows, and the weight of the sown grass seeds per square meter is 2 grams. Ensure that the soil is fully moist within 3 to 4 weeks after sowing. Once malignant weeds (such as sedge, reed, etc.) can be identified, they should be removed manually or mechanically immediately.

[0013] The nectar source plants of the present invention include at least one of the following: Patchouli, mint, alfalfa, sesame, sunflower, sweet buckwheat, cornflower, petunia, cosmos, spring rape, Potentilla, prickly ear, endive, broad bean, mud cabbage and summer solstice grass. The preferred combination is cornflower, sweet buckwheat, sunflower, sesame, broad bean and alfalfa.

[0014] In the integrated prevention and control of corn fields, the screened high-efficiency pesticides produce sublethal effects on corn borers, while the reduced amount of pesticides reduces the toxicity to natural enemies; the preferred nectar plant combination in the artificial flower and grass belt protects the natural enemy population and can enhance the biological control effect on corn; the chemical pesticides and the ecological regulation effect of the flower and grass belt together achieve a synergistic effect.

[0015] In order to further illustrate the present invention, an intensive green control method for corn pests in farmland provided by the present invention is described in detail below in conjunction with embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0016] Example 1

[0017] 1. Screening of highly effective and low-toxic chemical insecticides for corn borer

[0018] 1.1 Screening process

[0019] 1.1.1 Screening process of corn borer pesticides

[0020] The tested corn borers were reared indoors in succession in an insect-raising room with a temperature of (27±1)°C, a relative humidity of 50% to 75%, and a photoperiod of L / D=14h / 10h. During the experiment, 3rd instar larvae of the same age were selected for inoculation.

[0021] Test agents: The indoor determination used 95.3% chlorfenapyr technical, 95% fenpropimorph technical, 96% avermectin benzoate technical, 98% cypermethrin technical, 98% methoxyfenozide technical, 87% phoxim technical, and 10% azadirachtin extract.

[0022] The field test used the corresponding 200 g / L chlorantraniliprole suspension, 10% tetrachlorantraniliprole suspension, 5% avermectin benzoate soluble granules, 10% cypermethrin suspension, 24% methoxyfenozide suspension (Shandong United Pesticide Industry Co., Ltd.), 40% phoxim emulsifiable concentrate, and 0.3% azadirachtin emulsifiable concentrate.

[0023] Indoor toxicity test on corn borer: According to the "Guidelines for Indoor Bioassay Tests of Pesticides - Pesticides Part 10: Artificial Diet Mixture Method" (NY / T1154.10-2008).

[0024] Evaluation of the field control effect of corn borer: When corn borer holes are first seen in the field, spray the pesticide evenly and irrigate the heart leaves. The experiment has 15 treatments, and the application is 50L / mu of water:

[0025] Treatment 1, Treatment 2: 200g / L chlorfenapyr suspension 150, 225mL / hm 2 ;

[0026] Treatment 3, Treatment 4: 10% tetrachlorantraniliprole suspension 300, 450mL / hm 2 ;

[0027] Treatment 5, Treatment 6: 5% emamectin benzoate soluble granules 195, 390 g / hm 2 ;

[0028] Treatment 7, Treatment 8: 10% chlorfenapyr suspension 500, 1000 mL / hm 2 ;

[0029] Treatment 9, Treatment 10: 240g / L methoxyfenozide suspension 300, 450mL / hm 2 ;

[0030] Treatment 11, Treatment 12: 40% phoxim EC 1125, 1500mL / hm 2 ;

[0031] Treatment 13, Treatment 14: 0.3% azadirachtin emulsifiable concentrate 2.7, 4.5 g / hm 2 ;

[0032] Treatment 15: Clean water control.

[0033] Each treatment was repeated three times, and the plot area was 100m 2 , random block arrangement, corn planting row spacing of 0.6m, plant spacing of 26cm. Survey 30 days after spraying, sampling at 5 points in each plot, 50 corn plants at each point, the number of corn plants affected, the number of holes, the number of insects in the stalks, and the corresponding control effect were calculated.

[0034] Wormhole reduction rate = (number of control wormholes - number of treated wormholes) / number of control wormholes × 100%;

[0035] Damaged plant reduction rate = (number of damaged plants in the control group - number of damaged plants in the treatment group) / number of damaged plants in the control group × 100%;

[0036] Insect population reduction rate = (control insect population - treatment insect population) / control insect population × 100%;

[0037] The control effect 30 days after application of the medicine = (reduction rate of insect holes + reduction rate of damaged plants + reduction rate of overall pest population) / 3.

[0038] The Probit probability value analysis method was used to calculate the toxicity regression equation and LC 50 , 95% confidence interval.

[0039] The results are shown in Table 1. The most toxic compound is emamectin benzoate, LC 50 The value was 0.28mg / L; followed by chlorantraniliprole, tetrachlorantraniliprole, azadirachtin, cypermethrin, and methoxyfenozide; phoxim was the lowest, with LC 50 The value is 128.6mg / L.

[0040] Table 17 Indoor Toxicity of Pesticides to Corn Borer Larvae

[0041] Pharmacy Slope ± standard error <![CDATA[LC 50 (95%CL)(mg / L)]]> Chlorantraniliprole 0.95±0.14 0.50(0.25～0.9) Tetrachlorantraniliprole 0.92±0.16 0.51(0.26～1.0) Emamectin benzoate 1.21±0.15 0.28(0.1～0.4) Chlorfenapyr 1.44±0.25 8.2(5.4～13.4) Methoxyfenozide 0.96±0.12 98.3(51.4～210.8) Phoxim 1.34±0.21 128.6(80.1～208.5) Azadirachtin 2.03±0.35 6.4(4.9～8.4)

[0042] The control effects of seven insecticides on corn borer at ear stage 30 days after application are shown in Table 2. The control effects are ranked as follows: 200 g / L chlorantraniliprole suspension, 10% tetrachlorantraniliprole suspension, 0.3% azadirachtin emulsifiable concentrate, 5% avermectin benzoate soluble granules, 10% cypermethrin suspension, 40% phoxim emulsifiable concentrate and 240 g / L methoxyfenozide suspension.

[0043] Table 27 insecticides against corn borer at ear stage 30 days after application

[0044]

[0045]

[0046] 1.1.2 The process of drug screening for Trichogramma

[0047] Test reagents: Same as 1.1.

[0048] Natural enemies tested: Trichogramma ostriniae.

[0049] The toxicity of corn borer trichogrammatis was determined by the tube test film method. After preparing different concentration gradients of drug solution, add 1μL Tween 20 to each and shake it fully. Pipette 2mL of the prepared concentration drug solution into a 50mL finger-shaped tube, and the control group absorbs an equal amount of distilled water, then quickly rotate the finger-shaped tube to evenly coat the inner wall of the tube with the drug solution, and finally discard the excess drug solution, invert the finger-shaped tube on the filter paper, and dry it naturally in the room to form a drug film tube. Corn borer trichogrammatis is bred by rice moth eggs. Each drug film tube is connected to 5 rice moth eggs that are hatching. After 2h, the rice moth egg shells are quickly removed, and the pine trichogrammatis is retained. It is placed in a light incubator with a temperature of 25℃, a humidity of (75±1)%, and a D:L=16h:8h. After 24h, the number of trichogrammatis and the number of deaths (the trichogrammatis cannot crawl, which means death), and the mortality rate is calculated.

[0050] The indoor toxicity of the seven insecticides to Trichogramma ostriniae is shown in Table 3 and ranked as follows: avermectin benzoate, phoxim, cypermethrin, methoxyfenozide, tetrachlorfenapyr, chlorantraniliprole and azadirachtin.

[0051] Table 3 Indoor toxicity of 7 insecticides to Trichogramma ostriniae

[0052] Pharmacy Slope ± standard error <![CDATA[LC 50 (95%CL)(mg / L)]]> Chlorantraniliprole 1.47±0.21 177.9(96.8～264.2) Tetrachlorantraniliprole 1.45±0.15 171.4(92.3～254.1) Emamectin benzoate 2.44±0.18 28.1(20.5～34.8) Chlorfenapyr 1.12±0.11 78.6(60.2～95.8) Methoxyfenozide 1.41±0.19 161.5(82.8～244.1) Phoxim 2.11±0.15 35.2(26.7～42.1) Azadirachtin 1.55±0.28 185.6(99.1～260.1)

[0053] 2. Selection of flower and grass combinations and construction of artificial flower and grass belts

[0054] We screened nectar plants, and selected the best combinations of flowers and plants based on their ability to extend the life of parasitic bees, increase their reproductive capacity, and their ability to detoxify pesticides. We used the combination of flowers and plants to plant a 3-meter-wide artificial flower and plant belt at the edge of the corn field.

[0055] 2.1 Nectar Plants

[0056] The nectar plants include Patchouli, Mint, Alfalfa, Sesame, Sunflower, Sweet Buckwheat, Cornflower, Petunia, Cosmos, Spring Rape, Potentilla, Prickly Ear, Solanum, Broad Bean, Mud Cabbage, and Summer Solstice Grass. The flowers used for the experiment were conventionally cultivated in a glass greenhouse and used for the experiment after flowering.

[0057] 2.2 Beneficial effects of nectar / pollen feeding on Trichogrammatus ostriniae

[0058] 2.2.1 Lifespan and parasitism of Trichogrammatids after feeding on nectar / pollen

[0059] Take fresh flowers of nectar plants, moisturize the flower stalk with wet cotton balls, and then put them into a glass tube (5 cm long and 2 cm in diameter). At the same time, put an inactivated rice moth egg card (about 150 grains) and inoculate a female adult of Trichogrammatis ostriniae that has just emerged (<3h) and has mated in groups. The blank control does not put flowers, but uses wet cotton balls instead, and other conditions are the same. Observe the survival of Trichogrammatis ostriniae every 2h. Replace fresh blooming flowers every day and take out the egg cards placed the day before, and put in fresh egg cards. After the rice moth eggs turn black, record the number of black rice moth eggs (parasitized). The experiment was carried out in a climate incubator with a temperature of (28±1)℃, a relative humidity of 70% to 80%, and a photoperiod of 14h:10h (L / D). Each treatment was repeated 20 times.

[0060] The results are shown in Table 4. After female corn borer Trichogramma feed on plant nectar / pollen, the effect of extending and improving lifespan and parasitic ability is ranked as follows: cornflower>sunflower>sesame>sweet buckwheat>broad bean>caraway>marestail spinach>almond>alfalfa>rape>endaria>hippocampus>mint>potentilla>summer solstice grass>cosmos>morning glory.

[0061] Table 4 Differences in lifespan and parasitism of female adults of Trichogrammatus ostriniae feeding on different flowers

[0062]

[0063]

[0064] 2.3 Pesticide resistance and detoxification ability of Trichogrammatus ostriniae after feeding on nectar / pollen

[0065] The LC of chlorantraniliprole against Trichogramma ostriniae was determined by tube film method after the adult Trichogramma ostriniae was fed with nectar of different plants. 50 Values ​​are used to reflect the changes in the resistance of Trichogrammatus ostriniae to pesticides.

[0066] Chlorantraniliprole was diluted to a sublethal concentration (177.9 mg / mL) with acetone. 0.5 mL of chlorantraniliprole was drawn up using a pipette and slowly added to the finger-shaped tube (with an inner wall area of ​​54.6 cm 2 ), rotate the finger-shaped tube at a constant speed, and chlorantraniliprole is evenly distributed on the inner wall of the finger-shaped tube to form a drug film. After the drug solution is completely volatilized, 200 adult corn borer trichogrammatids fed with nectar from different plants are introduced into each finger-shaped tube. Seal the tube mouth with black cloth, place it in an artificial climate box (temperature 25±1℃, relative humidity 75%±5%, photoperiod 15L:9D) and culture it for 8 hours, and then collect 100 surviving adult bees. Quickly freeze it with liquid nitrogen and store it in a -80℃ refrigerator for later use. Take the samples to be treated in a homogenizer, add 1.2mL of pre-cooled 0.1mol / L phosphate buffer (pH7.4) respectively, and use a homogenizer to fully homogenize the specimens. After the homogenate is centrifuged at 4℃ and 10000g for 10 minutes, transfer the supernatant to a new centrifuge tube, and take the supernatant and put it on ice as enzyme solution for later use. Enzyme-linked immunosorbent assay (ELISA) was used to measure the enzyme activity of glutathione S-transferase to reflect the changes in the detoxification ability of Trichogramma ostriniae to pesticides.

[0067] Table 5 shows the LC of chlorantraniliprole against Trichogrammatis oryzae after Trichogrammatis ostriniae feeds on plant nectar / pollen. 50 The order of value size is: cornflower>sweet buckwheat>sunflower>sesame>broad bean>alfalfa>prickly lettuce>mud husk>spring rape>endarian>patches>mint>summer solstice grass>potentilla>morning glory>cosmos. The order of detoxification ability is: cornflower>sweet buckwheat>sunflower>sesame>broad bean>alfalfa>prickly lettuce>mud husk>spring rape>endarian>patches>mint>summer solstice grass>potentilla>morning glory>cosmos.

[0068] Table 5 Drug resistance and detoxification ability of Trichogramma ostriniae adults feeding on different flowers

[0069]

[0070]

[0071] 2.4 Optimization of flower and grass combinations and construction of flower and grass belts for people

[0072] The optimized flower and grass combination is: cornflower, sweet buckwheat, sunflower, sesame, broad bean and alfalfa. When preparing the land at the edge of the farmland, the width of the plant buffer zone is 2-4 meters. If the field is square, the plant buffer zone is constructed along the long side of the plot. If the field is irregular, the plant buffer zone is constructed along the perimeter of the plot. Before planting, 40 kg of compound fertilizer (N: P: K = 15: 15: 15) is applied per mu as base fertilizer. When sowing in the autumn of the first year, the perennial plants cornflower and alfalfa are mixed in equal proportions by mass and the grass seeds are sown with a hand-cranked seeder. The weight of the sown grass seeds per square meter is 5 grams. After sowing, the soil is covered with 1 cm and the soil is compacted with a compactor. When sowing in the spring of the second year, the annual plants sweet buckwheat, sunflower, sesame, and broad bean are mixed in equal proportions by mass and the grass seeds are sown in rows. The weight of the sown grass seeds per square meter is 2 grams. Ensure that the soil is fully moist within 3 to 4 weeks after sowing. Once malignant weeds (such as sedge, reed, etc.) can be identified, they should be removed manually or mechanically immediately.

[0073] 3. The reduction of chemical pesticide use and the construction of artificial flower and grass belts have a synergistic effect

[0074] The experiment was conducted in Zhouzhuang Village, Meichang Town, Wuqing District, Tianjin (117.219896 east longitude, 39.364681 north latitude). The test corn variety was 'Xianyu 335', ​​with a row spacing of 0.6m and a plant spacing of 26cm. The experimental plot was 200 meters long and 100 meters wide. A 3-meter-wide artificial flower and grass belt was built on the edge of one long side in the autumn of 2021. The flower and grass combination was cornflower, sweet buckwheat, sunflower, sesame, broad bean and alfalfa. When sowing in the autumn of the first year, the perennial plants cornflower and alfalfa were mixed in equal proportions by mass and the grass seeds were sown by hand-cranked seeder, with a sowing weight of 5 grams per square meter; when sowing in the spring of the second year, the annual plants sweet buckwheat, sunflower, sesame and broad bean were mixed in equal proportions by mass and the grass seeds were sown in rows, with a sowing weight of 2 grams per square meter. Crops were planted normally on the edge of the other long side, which was a control without a flower and grass belt.

[0075] The recommended field dose of 200g / L chlorantraniliprole suspension, a highly effective pesticide for corn borers, is 10mL / mu. If diluted with 50L of water, the recommended concentration is 40mg / L. Five gradient doses are set, namely 0.004, 0.04, 0.4, 4, and 40mg / L, and a blank control of clean water, for a total of 6 treatments. Each treatment is repeated 3 times, and each repeated plot is 10m*10m, with a spacing of 1m between plots. Artificial spraying treatment is carried out during the peak hatching period of corn borer eggs, and the spraying time is August 1, 2023.

[0076] The investigation was carried out 30 days after the application of the pesticide. Five sampling points were taken in each plot, and 50 corn plants were investigated at each point. The number of damaged corn plants, the number of bored holes, and the number of insect populations were investigated by dissecting the stalks, and the corresponding control effects and LC of chlorantraniliprole on corn were calculated. 50 value.

[0077] Referring to the method of co-toxicity coefficient of pesticides, the synergistic coefficient (CIC value) of the combined measures was calculated according to the following formula:

[0078] The combined measure (CM) included the chlorantraniliprole measure A1 and the flower and grass belt measure A2. Each measure had its own control effect value on the Asian corn borer. The improvement index of measure A1 was set as the standard value of 100.

[0079] The improvement index of A2 = the control effect value of A2 / the control effect value of A1 × 100;

[0080] The actual improvement index of CM = the control effect value of CM / the control effect value of A1 × 100;

[0081] The theoretical improvement index of CM = the improvement index of A1 + the improvement index of A2;

[0082] The synergistic coefficient CIC value = the actual improvement index of CM / the theoretical improvement index of CM × 100;

[0083] CIC ≥ 120 indicates a synergistic effect; CIC ≤ 80 indicates an antagonistic effect; 80 < CIC < 120 indicates an additive effect.

[0084] As shown in Table 6, the CIC values of "with flower and grass belt + chlorantraniliprole 0.4 mg / L" and "with flower and grass belt + chlorantraniliprole 4 mg / L" were both greater than 120, and these two combined measures both had a synergistic effect. The control effect of "with flower and grass belt + chlorantraniliprole 4 mg / L" on the Asian corn borer reached 100%, and the dosage of chlorantraniliprole in the combined measure was only 1 / 10 of that of single chemical control, making the farmland ecological environment safer.

[0085] Table 6 Effects of the combination of chlorantraniliprole and artificial flower and grass belt

[0086]

[0087]

[0088] Although the above embodiments have described the present invention in detail, they are only a part of the embodiments of the present invention, not all of them. People can also obtain other embodiments based on these embodiments without creative efforts, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A method for green control of corn pests in intensive farmland, It is characterized in that The following steps are involved: (1) Select insecticide varieties that are highly effective and low in toxicity against major corn pests and reduce the application rate by at least half; (2) constructing an artificial flower and grass belt beside a corn field, wherein the artificial flower and grass belt contains nectar plants that are natural enemies of major corn pests.

2. The method according to claim 1, It is characterized in that The main corn pests include lepidopteran pests, and the screened high-efficiency and low-toxic insecticide varieties include at least one of the following: avermectin benzoate, chlorantraniliprole, tetrachlorantraniliprole, azadirachtin, cypermethrin, methoxyfenozide and phoxim.

3. The method according to claim 1, It is characterized in that The at least halved application in step (1) includes reducing the application amount of the screened pesticide variety by at least half of the amount specified in the pesticide manual.

4. The method according to claim 1, It is characterized in that In step (2), the artificial flower and grass belt is constructed at the edge of the farmland, and the width of the artificial flower and grass belt is 2 to 4 meters.

5. The method according to claim 4, It is characterized in that Before constructing the artificial flower and grass belt, seeds of perennial nectar source plants are sown in the autumn of the first year, and seeds of annual nectar source plants are sown in the spring of the second year.

6. The method according to claim 5, It is characterized in that The sowing rate of the seeds of the perennial nectar source plant is 5g / m 2 The sowing rate of the seeds of the annual nectar source plant is 2g / m 2 .

7. The method according to claim 4 or 5, It is characterized in that The nectar source plants include at least one of the following: Patchouli, mint, alfalfa, sesame, sunflower, sweet buckwheat, cornflower, petunia, cosmos, spring rape, cinquefoil, thorny ear, somniferum, broad bean, mud cabbage and summer solstice grass.

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