A kind of premixed feed for dairy cows in lactation period and its preparation method and application

Abstract

The present invention belongs to the technical field of animal feed, and specifically relates to a premixed feed for dairy cows during lactation, and a preparation method and application thereof. The feed composition of the present invention comprises the following components: a carrier, minerals, probiotics, allicin and Aspergillus oryzae fermentation complex enzyme. Adding minerals to the premixed feed composition for lactating dairy cows of the present invention can reduce the number of somatic cells in milk, effectively reducing the risk of diseases such as mastitis in dairy cows; probiotics can regulate the ecological conditions of rumen microorganisms and promote their production performance; allicin can promote the growth of dairy cows, increase milk production and milk fat rate, and reduce feeding costs; Aspergillus oryzae fermentation complex enzyme can alleviate heat stress in dairy cows. The formula design of the present invention is scientific and reasonable, and the nutrition is comprehensive and balanced. It can provide balanced nutrition for lactating dairy cows, help improve the resistance and immune function of lactating dairy cows, improve the quality of milk, and improve the economic benefits of farmers.

Description

Technical Field

[0001] The invention belongs to the technical field of animal feed, and in particular relates to a premixed feed for dairy cows in lactation period, and a preparation method and application thereof. Background Art

[0002] The feeding and management of lactating dairy cows is not only directly related to the delivery, health and reproduction level of dairy cows, but also the milk production and milk quality during the entire lactation period. It is also the core work of the dairy farm, directly affecting the economic benefits and guaranteeing the sustainable development of the dairy farm. The ratio of coarse and concentrated feed in dairy cow feed is very important for dairy cows. Too much concentrated feed will reduce the pH of the rumen, increase the production of propionic acid, and reduce the production of acetic acid, which will increase the nutrient supply and milk production, but the efficiency of synthetic milk fat is low and the milk fat rate decreases. In addition, excessive feeding of concentrated feed will also cause rumen poisoning, bait laminitis, infertility and other diseases. Studies have shown that high-yielding dairy cows need to reduce the negative nutritional balance as much as possible and ensure the normal function of the rumen to maintain the health of dairy cows and ensure stable and high production.

[0003] Chinese invention patent CN103229898A discloses a dairy cow feed and a preparation method thereof, which is composed of the following raw materials in parts by weight: 40-50 parts of rape straw, 20-30 parts of astragalus, 15-25 parts of sweet potato leaves, 15-20 parts of cottonseed hulls, 10-15 parts of peanut vines, 15-20 parts of flour, 10-15 parts of corn grits, 1-2 parts of leavening agent, 40-45 parts of oatmeal, 20-25 parts of sorghum bran, 15-20 parts of fried rice flour, 3-5 parts of soft candy additives, 4-5 parts of rice bran, 5-8 parts of clay-like sepiolite, 3-4 parts of palm oil, 2-3 parts of sodium alginate, 1-2 parts of calcium carbonate, 1-1.5 parts of sodium hydrogen phosphate and 0.2-0.4 parts of salt. The feed of the invention contains various nutrients required for the growth of dairy cows, and while ensuring their healthy production, it also provides health care value and improves the growth rate of dairy cows.

[0004] Another Chinese invention patent CN104585531A discloses a lactating cow feed for promoting the growth and lactation of dairy cows. The raw materials include, by weight: 30-33 parts of corn flour, 30-33 parts of sorghum flour, 14-17 parts of prefabricated active materials, 12-15 parts of konjac, 8-12 parts of wheat flour, 7-10 parts of barley bran, 5-8 parts of straw powder, 3-5 parts of dry beet residue, 5-8 parts of carrots, 6-9 parts of fish bone powder, 6-9 parts of whey powder, 3.5-5 parts of soybean oil, 2-4 parts of salt, 2-2.5 parts of brown sugar, 1-2 parts of calcium hydrogen phosphate, 0.1-0.3 parts of lysine, 0.2-0.5 parts of choline, 0.1-0.2 parts of cod liver oil, and 0.06-0.1 parts of magnesium sulfate. The additives of the invention are natural and pollution-free, and have obvious effects in promoting the growth of dairy cows and increasing milk secretion.

[0005] However, the above-mentioned dairy cow feed formula has certain limitations in its purpose and adaptability, and cannot fully meet the growth needs of dairy cows. Therefore, in order to improve a series of problems such as decreased feed intake, negative energy balance and decreased immune function of dairy cows, the field urgently needs to develop a feed with a reasonable formula that can improve the resistance and immune function of dairy cows, which is of great significance for improving milk quality and the economic benefits of farmers. Summary of the invention

[0006] The present invention aims at the problems existing in the prior art and provides a premixed feed for dairy cows in lactation period and a preparation method and application thereof. The present invention minimizes the influence of physiological factors on the body, adds nutrients such as mineral elements, water-soluble allicin and probiotics, supplements balanced nutrition for dairy cows, helps dairy cows digestion and absorption, body recovery and promotes fetal growth and development, improves the resistance and immune function of lactating dairy cows, maintains the health of dairy cows, and lays a foundation for the perinatal period and even the lactation period.

[0007] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0008] A feed composition comprises the following components: a carrier, probiotics, minerals, allicin and Aspergillus oryzae fermentation complex enzyme.

[0009] Preferably, the carrier is rice husk powder, and the mineral is selected from one or more of calcium carbonate, calcium hydrogen phosphate, magnesium sulfate, sodium chloride, ferrous sulfate heptahydrate, zinc sulfate heptahydrate, copper sulfate pentahydrate, manganese sulfate pentahydrate, selenium-enriched yeast and cobalt chloride hexahydrate.

[0010] Preferably, the probiotics are selected from one or more of Bacillus paralicheniformis, Bacillus amyloliquefaciens and Bacillus subtilis.

[0011] More preferably, the probiotics are a mixture of Bacillus paralicheniformis and Bacillus amyloliquefaciens in a weight ratio of 1-1.5:0.5-2.

[0012] Preferably, the composition further comprises lysine, methionine, Saccharomyces cerevisiae, an antioxidant and chromium propionate.

[0013] Preferably, the antioxidant is ethoxyquinoline, butylated hydroxytoluene or propyl gallate.

[0014] Preferably, the composition comprises the following components by weight percentage: 1-5% carrier, 0.01-0.05% antioxidant, 0.01-0.05% probiotic, 0.1-0.5% allicin, 70-80% minerals, 8-12% lysine, 1-5% methionine, 1-5% brewer's yeast, 1-5% Aspergillus oryzae fermentation complex enzyme, and 0.01-0.05% chromium propionate.

[0015] More preferably, the composition comprises the following components by weight percentage: 4-5% carrier, 0.01-0.02% antioxidant, 0.03-0.05% probiotic, 0.1-0.2% allicin, 70-75% mineral, 9-11% lysine, 4-5% methionine, 2-4% brewer's yeast, 3-5% Aspergillus oryzae fermentation complex enzyme, and 0.04-0.05% chromium propionate.

[0016] The present invention also provides a method for preparing the feed composition, comprising the following steps: weighing minerals, carriers, antioxidants, probiotics, allicin, lysine, methionine, brewer's yeast, Aspergillus oryzae fermentation complex enzyme and chromium propionate, mixing for 20-40 minutes, packaging, and inspecting.

[0017] The invention also provides a premixed feed for dairy cows in the lactation period, the components of which include the feed composition.

[0018] The present invention also provides application of the feed composition or the premixed feed for dairy cows in lactation period in dairy cow breeding.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] (1) The premixed feed composition for lactating dairy cows of the present invention has a more scientific and reasonable formula design and balanced nutrition, which can provide balanced nutrition for lactating dairy cows, help improve the resistance and immune function of lactating dairy cows, help improve the quality of milk, and improve the economic benefits of farmers.

[0021] (2) The minerals added to the feed of the present invention are safe and easy to absorb, can reduce the somatic cell count in milk, and effectively reduce the risk of diseases such as mastitis in dairy cows; allicin is a plant broad-spectrum antibiotic with the characteristics of low residue, low cost, strong appetizing and flavoring properties, no drug resistance, and can promote the growth of dairy cows, increase milk production and milk fat rate, prevent and treat related diseases, and reduce feeding costs; probiotics can regulate the ecological conditions of rumen microorganisms, promote their production performance, improve feed utilization, and improve the health of ruminants; coated lysine and methionine can increase the milk production of dairy cows, improve metabolic disorders and improve reproductive performance; brewer's yeast can enhance the production performance of dairy cows; Aspergillus oryzae fermentation complex enzyme and chromium propionate relieve heat stress in dairy cows and improve the problem of decreased feed intake and milk production in dairy cows caused by heat stress.

[0022] (3) The mixed feed composition for lactating dairy cows provided by the present invention has been shown to have obvious improvement effects through animal experiments. Feeding dairy cows with the premixed feed for lactating dairy cows of the present invention is beneficial to the rapid absorption of various special energy substances, and can increase milk production and improve milk quality, and can effectively increase the content of milk fat, milk protein, lactose and milk dry matter in milk. DETAILED DESCRIPTION

[0023] The following non-limiting examples can enable those of ordinary skill in the art to more fully understand the present invention, but do not limit the present invention in any way. The following content is merely an exemplary description of the scope of the present invention, and those skilled in the art can make various changes and modifications to the invention of the present invention according to the disclosed content, and they should also fall within the scope of the present invention. Unless otherwise stated, the materials or reagents provided in the examples are all common commercial products, and the following is only an exemplary description.

[0024] Bacillus paralicheniformis is Bacillus paralicheniformis SN-6 strain, and Bacillus amyloliquefaciens is Bacillus amyloliquefaciens SN-C strain, both of which are prepared with reference to Example 1 of invention patent CN111996139A; Aspergillus niger, CICC40048, is purchased from China Industrial Microbiological Culture Collection Management Center; allicin, with a purity of 98%, is purchased from Jinan Luya Biotechnology Co., Ltd.; lysine is L-lysine hydrochloride, with a purity of 99%, purchased from Wuhan Baixing Biotechnology Co., Ltd.; methionine is DL-methionine, with a purity of 99%, purchased from Evonik Industries AG; Saccharomyces cerevisiae is a culture of Saccharomyces cerevisiae purchased from Cangzhou Oriental Veterinary Drug Co., Ltd.; Aspergillus oryzae fermentation complex enzyme, with a purity of 95%, is purchased from Shandong Hezhong Kangyuan Biotechnology Co., Ltd.; chromium propionate, with a purity of 98%, is purchased from Xi'an Zhanxun Biotechnology Co., Ltd.

[0025] Example 1

[0026] A premixed feed for dairy cows in lactation period, with a total weight of raw materials of 1000g, composed of the following components: 48.3g rice husk powder, 0.1g butylated hydroxytoluene, 0.4g probiotics (Bacillus paralicheniformis SN-6 strain and Bacillus amyloliquefaciens SN-C strain in a weight ratio of 1:1), 1.0g allicin, 740.2g calcium carbonate, 101.0g lysine, 40.4g methionine, 28.4g brewer's yeast, 39.8g Aspergillus oryzae fermentation complex enzyme, and 0.4g chromium propionate.

[0027] A method for preparing a premixed feed for dairy cows in the lactation period comprises the following steps:

[0028] Weigh the corresponding weight of rice husk powder, butylated hydroxytoluene, probiotics, allicin, calcium carbonate, lysine, methionine, brewer's yeast, Aspergillus oryzae fermentation complex enzyme and chromium propionate, put them into a mixing tank, mix them thoroughly for 30 minutes, and package them after the mixing is completed. The packaged products can be put into storage after passing the inspection.

[0029] Example 2

[0030] A premixed feed for dairy cows in lactation period, with a total weight of 1000g of raw materials, is composed of the following components: 50g of rice husk powder, 0.5g of propyl gallate, 0.5g of probiotics (Bacillus paralicheniformis SN-6 strain and Bacillus amyloliquefaciens SN-C strain in a weight ratio of 1:1.5), 5.0g of allicin, 693.5g of magnesium sulfate, 120g of lysine, 50g of methionine, 30g of brewer's yeast, 50g of Aspergillus oryzae fermentation complex enzyme, and 0.5g of chromium propionate.

[0031] A method for preparing a premixed feed for dairy cows in the lactation period comprises the following steps:

[0032] Weigh the corresponding weight of rice husk powder, propyl gallate, probiotics, allicin, magnesium sulfate, lysine, methionine, brewer's yeast, Aspergillus oryzae fermentation complex enzyme and chromium propionate, put them into a mixing tank, mix them thoroughly for 20 minutes, and package them after the mixing is completed. The packaged products can be put into storage after passing the inspection.

[0033] Example 3

[0034] A premixed feed for dairy cows in lactation period has a total raw material weight of 1000g and is composed of the following components: 20g rice husk powder, 0.1g ethoxyquinoline, 0.1g probiotics (Bacillus paralicheniformis SN-6 strain and Bacillus amyloliquefaciens SN-C strain in a weight ratio of 1.5:1), 1.0g allicin, 800g zinc sulfate heptahydrate, 80g lysine, 28.7g methionine, 50g brewer's yeast, 20g Aspergillus oryzae fermentation complex enzyme, and 0.1g chromium propionate.

[0035] A method for preparing a premixed feed for dairy cows in the lactation period comprises the following steps:

[0036] Weigh the corresponding weight of rice husk powder, ethoxyquinoline, probiotics, allicin, zinc sulfate heptahydrate, lysine, methionine, brewer's yeast, Aspergillus oryzae fermentation complex enzyme and chromium propionate, put them into a mixing tank together, mix them thoroughly for 40 minutes, and package them after the mixing is completed. The packaged products can be put into storage after passing the inspection.

[0037] Example 4

[0038] A premixed feed for lactating dairy cows has a total raw material weight of 1000 g and is composed of the following components: 40 g rice husk powder, 0.3 g ethoxyquinoline, 0.3 g probiotics (Bacillus paralicheniformis SN-6 strain and Bacillus amyloliquefaciens SN-C strain in a weight ratio of 1:2), 3.0 g allicin, 729 g cobalt chloride hexahydrate, 112 g lysine, 45 g methionine, 30 g brewer's yeast, 40 g Aspergillus oryzae fermentation complex enzyme, and 0.4 g chromium propionate.

[0039] A method for preparing a premixed feed for dairy cows in the lactation period comprises the following steps:

[0040] Weigh the corresponding weight of rice husk powder, ethoxyquinoline, probiotics, allicin, cobalt chloride hexahydrate, lysine, methionine, brewer's yeast, Aspergillus oryzae fermentation complex enzyme and chromium propionate, put them into a mixing tank, mix them thoroughly for 40 minutes, and package them after the mixing is completed. The packaged products can be put into storage after passing the inspection.

[0041] Comparative Example 1

[0042] Compared with Example 1, the only difference is that allicin is replaced by antimicrobial peptide.

[0043] A premixed feed for dairy cows in lactation period, with a total weight of raw materials of 1000g, composed of the following components: 48.3g rice husk powder, 0.1g butylated hydroxytoluene, 0.4g probiotics (Bacillus paralicheniformis SN-6 strain and Bacillus amyloliquefaciens SN-C strain in a weight ratio of 1:1), 1.0g antimicrobial peptide, 740.2g calcium carbonate, 101.0g lysine, 40.4g methionine, 28.4g brewer's yeast, 39.8g Aspergillus oryzae fermentation complex enzyme, and 0.4g chromium propionate.

[0044] A method for preparing a premixed feed for dairy cows in the lactation period comprises the following steps:

[0045] Weigh the corresponding weight of rice husk powder, butylated hydroxytoluene, probiotics, antimicrobial peptides, calcium carbonate, lysine, methionine, brewer's yeast, Aspergillus oryzae fermentation complex enzyme and chromium propionate, put them into a mixing tank, mix them thoroughly for 30 minutes, and package them after the mixing is completed. The packaged products can be put into storage after passing the inspection.

[0046] Comparative Example 2

[0047] Compared with Example 1, the only difference is that no probiotics are contained.

[0048] A premixed feed for dairy cows in lactation period has a total raw material weight of 1000 g and is composed of the following components: 48.3 g rice husk powder, 0.1 g butylated hydroxytoluene, 1.0 g allicin, 740.6 g calcium carbonate, 101.0 g lysine, 40.4 g methionine, 28.4 g brewer's yeast, 39.8 g Aspergillus oryzae fermentation complex enzyme, and 0.4 g chromium propionate.

[0049] A method for preparing a premixed feed for dairy cows in the lactation period comprises the following steps:

[0050] Weigh the corresponding weight of rice husk powder, butylated hydroxytoluene, allicin, calcium carbonate, lysine, methionine, brewer's yeast, Aspergillus oryzae fermentation complex enzyme and chromium propionate, put them into a mixing tank, mix them thoroughly for 30 minutes, and package them after the mixing is completed. The packaged products can be put into storage after passing the inspection.

[0051] Comparative Example 3

[0052] Compared with Example 1, the only difference is that the Bacillus paralicheniformis SN-6 strain and the Bacillus amyloliquefaciens SN-C strain are replaced by Aspergillus niger.

[0053] A premixed feed for dairy cows in lactation period has a total raw material weight of 1000g and is composed of the following components: 48.3g rice husk powder, 0.1g butylated hydroxytoluene, 0.4g probiotics (Aspergillus niger), 1.0g allicin, 740.2g calcium carbonate, 101.0g lysine, 40.4g methionine, 28.4g brewer's yeast, 39.8g Aspergillus oryzae fermentation complex enzyme, and 0.4g chromium propionate.

[0054] A method for preparing a premixed feed for dairy cows in the lactation period comprises the following steps:

[0055] Weigh the corresponding weight of rice husk powder, butylated hydroxytoluene, probiotics, allicin, calcium carbonate, lysine, methionine, brewer's yeast, Aspergillus oryzae fermentation complex enzyme and chromium propionate, put them into a mixing tank, mix them thoroughly for 30 minutes, and package them after the mixing is completed. The packaged products can be put into storage after passing the inspection.

[0056] Comparative Example 4

[0057] Compared with Example 1, the only difference is that the weight ratio of Bacillus paralicheniformis SN-6 to Bacillus amyloliquefaciens SN-C is 1:3.

[0058] A premixed feed for dairy cows in lactation period, with a total weight of raw materials of 1000g, composed of the following components: 48.3g rice husk powder, 0.1g butylated hydroxytoluene, 0.4g probiotics (Bacillus paralicheniformis SN-6 strain and Bacillus amyloliquefaciens SN-C strain in a weight ratio of 1:3), 1.0g allicin, 740.2g calcium carbonate, 101.0g lysine, 40.4g methionine, 28.4g brewer's yeast, 39.8g Aspergillus oryzae fermentation complex enzyme, and 0.4g chromium propionate.

[0059] A method for preparing a premixed feed for dairy cows in the lactation period comprises the following steps:

[0060] Weigh the corresponding weight of rice husk powder, butylated hydroxytoluene, probiotics, allicin, calcium carbonate, lysine, methionine, brewer's yeast, Aspergillus oryzae fermentation complex enzyme and chromium propionate, put them into a mixing tank, mix them thoroughly for 30 minutes, and package them after the mixing is completed. The packaged products can be put into storage after passing the inspection.

[0061] Comparative Example 5

[0062] Compared with Example 1, the only difference is that the weight ratio of Bacillus paralicheniformis SN-6 to Bacillus amyloliquefaciens SN-C is 3:1.

[0063] A premixed feed for lactating dairy cows has a total raw material weight of 1000 g and is composed of the following components: 48.3 g rice husk powder, 0.1 g butylated hydroxytoluene, 0.4 g probiotics (Bacillus paralicheniformis SN-6 strain and Bacillus amyloliquefaciens SN-C strain in a weight ratio of 3:1), 1.0 g allicin, 740.2 g calcium carbonate, 101.0 g lysine, 40.4 g methionine, 28.4 g brewer's yeast, 39.8 g Aspergillus oryzae fermentation complex enzyme, and 0.4 g chromium propionate.

[0064] A method for preparing a premixed feed for dairy cows in the lactation period comprises the following steps:

[0065] Weigh the corresponding weight of rice husk powder, butylated hydroxytoluene, probiotics, allicin, calcium carbonate, lysine, methionine, brewer's yeast, Aspergillus oryzae fermentation complex enzyme and chromium propionate, put them into a mixing tank, mix them thoroughly for 30 minutes, and package them after the mixing is completed. The packaged products can be put into storage after passing the inspection.

[0066] Effect example

[0067] 1. Experimental Materials and Methods

[0068] (1) Experimental time and location

[0069] The dairy cow trial was completed in 2022 at the dairy farm of Wuhan Jinxu Animal Husbandry Technology Development Co., Ltd. in Hubei Province.

[0070] (2) Experimental animals and groups

[0071] A total of 110 Holstein cows of similar age, weight, parity and lactation days were selected for the experiment. Ten healthy cows with somatic cell counts less than 200,000 / mL were set as the healthy group, and 100 cows with clinical mastitis with somatic cell counts greater than 500,000 / mL were randomly divided into 10 groups, mastitis groups and experimental groups (premixed feeds for lactation of dairy cows prepared in Examples 1-4 and Comparative Examples 1-5). The cows in the healthy group and the cows in the mastitis group were fed a basal diet every day (the basal diet was prepared with reference to NY / T 34-2004 "Standards for Feeding Dairy Cows"), and the cows in the experimental group were fed with 0.05% of the premixed feeds for lactation of dairy cows prepared in Examples 1-4 and Comparative Examples 1-5 on the basis of the basal diet every day, and the experimental period was 40 days in total.

[0072] (3) Sample collection and preservation

[0073] On the morning of the 0th and 40th day of the test period, the blood of the cows was collected by blood sampling from the root vein of the tail when the cows did not eat. 10mL was collected from each cow. During the blood collection process, the blood was slowly drawn out with a syringe and placed in a 15mL centrifuge tube to avoid hemolysis. The centrifuge tube with blood samples was centrifuged at a speed of 3000r / min for 15min, and the upper serum was sucked out and placed in a centrifuge tube. The serum obtained after centrifugation of each cow was placed in 2 tubes on average. All serum samples were stored in a -80℃ refrigerator. During morning and evening milking on the 0th, 20th and 40th days, milk samples of cows were collected in 50mL centrifuge tubes, mixed according to the volume ratio of morning: evening = 4:6, and 0.05g potassium dichromate powder was added to each 50mL milk. After shaking well, it was placed in a 4℃ refrigerator for testing.

[0074] 2. Test indicators and testing methods

[0075] 2.1 Determination of milk production and milk quality of dairy cows

[0076] All experimental cows were milked twice a day (05:30 in the morning and 17:30 in the afternoon). The cows automatically recorded their milk production when using the herringbone milking platform. During milking, the milking workers recorded the milk production of all cows. The sum of the milk production twice a day was the milk production of each cow on that day. The collected milk samples were sent to the Hubei Dairy Cow Production Performance Measurement Center (Hubei, Wuhan) within 48 hours. The milk protein, milk fat, lactose, non-milk fat solids and total solids in the milk were detected using a milk component analyzer (FT600, Foss Electric, Denmark). The somatic cell count of the above milk samples was performed using a somatic cell counter (Somacout 150) while the milk was routinely measured.

[0077] 2.2 Serum biochemical indexes

[0078] The corresponding bovine ELISA kits from ELISA Biotechnology Co., Ltd. were used to detect prolactin, luteinizing hormone, immunoglobulin A and progesterone in the blood.

[0079] 2.3 Antioxidant index of dairy cows

[0080] Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), total cholesterol (T-AOC) and malondialdehyde (MDA) were detected using an enzyme-labeled detector (USCNK, SMR60047) according to the method provided by the kit of Nanjing Jiancheng Company.

[0081] 3. Experimental results and analysis

[0082] 3.1 Effect of premixed feed on production performance of dairy cows with mastitis

[0083] As shown in Table 1, the daily milk production of cows in the healthy group and the experimental group was significantly higher than that of cows in the mastitis group. The daily milk production of cows prepared by the premixed feeds of Examples 1-4 in the experimental group was higher than that of the comparative examples 1-5, indicating that the premixed feed of the present invention can increase the daily milk production of cows with mastitis.

[0084] As shown in Table 2, the quality of milk in the healthy group is significantly higher than that in the mastitis group. Compared with the mastitis group, Examples 1-4 in the experimental group can significantly increase the content of milk fat, milk protein and lactose.

[0085] Table 1 Effect of the premixed feed of the present invention on daily milk production and milk somatic cell count of mastitis cows

[0086]

[0087]

[0088] Note: The items marked with different letters in the above table are significantly different.

[0089] Table 2 Effect of premixed feed of the present invention on milk quality

[0090]

[0091] Note: The items marked with different letters in the above table are significantly different.

[0092] 3.2 Effects of premixed feed on serum biochemical parameters of dairy cows with mastitis

[0093] It can be seen from Tables 3-4 that from day 0 to day 40, the prolactin, progesterone and immunoglobulin A of the cows in the healthy group and the experimental group were significantly higher than those in the mastitis group. Among them, the premixed feed prepared in Examples 1-2 in the experimental group had higher luteinizing hormone, prolactin, progesterone and immunoglobulin A for cows than comparison examples 1, 3 and 4.

[0094] Table 3 Effect of the premixed feed of the present invention on the content of luteinizing hormone and prolactin in the serum of cows with mastitis

[0095]

[0096]

[0097] Table 4 Effect of the premixed feed of the present invention on the content of progesterone and immunoglobulin A in the serum of cows with mastitis

[0098]

[0099] Note: The items marked with different letters in the above table are significantly different.

[0100] 3.3 Effect of premixed feed on antioxidant function in serum of cows with mastitis

[0101] As shown in Tables 5-6, from day 0 to day 40, the superoxide dismutase, catalase and malondialdehyde contents of the cows in the mastitis group and the experimental group were no different from those of the cows in the healthy group. On day 0, the total antioxidant capacity of the cows in the mastitis group and the experimental group was lower than that of the cows in the healthy group. On day 40, the total antioxidant capacity of the cows in the experimental group was significantly higher than that of the mastitis group, among which the premixed feed prepared in Examples 1, 3 and 4 of the experimental group had higher superoxide dismutase, catalase content and total antioxidant capacity in the serum of cows with mastitis than those in Comparative Examples 1, 3 and 5.

[0102] Table 5 Effect of the premixed feed of the present invention on the contents of superoxide dismutase, glutathione peroxidase and catalase in the serum of cows with mastitis

[0103]

[0104] Table 6 Effect of the premixed feed of the present invention on the total antioxidant capacity and propylene glycol content in the serum of mastitis cows

[0105]

[0106]

[0107] Note: The items marked with different letters in the above table are significantly different.

[0108] 4. Experimental conclusion

[0109] After a dairy cow suffers from mastitis, milk production, milk protein rate, milk fat rate, lactose rate and non-fat solid rate decrease to varying degrees, the body's immune function and antioxidant capacity are inhibited, and the total antioxidant capacity in the serum is significantly reduced. After feeding the premixed feed of the present invention to the dairy cows with mastitis, the production performance, immune ability and antioxidant level are significantly improved, and the cow's mastitis is improved.

[0110] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. A premixed feed for dairy cows during lactation period, characterized in that: The composition includes the following components by weight percentage: carrier 1-5%, antioxidant 0.01-0.05%, probiotic 0.01-0.05%, allicin 0.1-0.5%, mineral 70-80%, lysine 8-12%, methionine 1-5%, brewer's yeast 1-5%, Aspergillus oryzae fermentation complex enzyme 1-5%, chromium propionate 0.01-0.05%, The probiotics are a mixture of Bacillus paralicheniformis and Bacillus amyloliquefaciens in a weight ratio of 1-1.5:1-2.

2. The premixed feed for lactating cows according to claim 1, characterized in that: The carrier is rice husk powder, and the mineral is selected from one or more of calcium carbonate, calcium hydrogen phosphate, magnesium sulfate, sodium chloride, ferrous sulfate heptahydrate, zinc sulfate heptahydrate, copper sulfate pentahydrate, manganese sulfate pentahydrate, selenium-enriched yeast and cobalt chloride hexahydrate.

3. The premixed feed for dairy cows in lactation period according to claim 1, wherein the antioxidant is ethoxyquinoline, butylated hydroxytoluene or propyl gallate.

4. The premixed feed for lactating cows according to claim 1, characterized in that: The composition includes the following components by weight percentage: 4-5% of carrier, 0.01-0.02% of antioxidant, 0.03-0.05% of probiotic, 0.1-0.2% of allicin, 70-75% of minerals, 9-11% of lysine, 4-5% of methionine, 2-4% of brewer's yeast, 3-5% of Aspergillus oryzae fermentation complex enzyme and 0.04-0.05% of chromium propionate.

5. A method for preparing a premixed feed for dairy cows during lactation as claimed in any one of claims 1 to 4, characterized in that: The following steps are included: weigh The minerals, carriers, antioxidants, probiotics, allicin, lysine, methionine, brewer's yeast, Aspergillus oryzae fermentation complex enzyme and chromium propionate are mixed for 20-40 minutes, packaged and inspected.

6. Use of the premixed feed for lactating dairy cows as claimed in any one of claims 1 to 4 or the premixed feed for lactating dairy cows prepared by the preparation method according to claim 5 in dairy cow breeding.

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