A reaction device for preparing bone-derived polypeptides by fermentation of cattle bones

Abstract

The present invention discloses a reaction device for preparing bone-derived polypeptides by fermenting cattle bones, and belongs to the technical field of polypeptide extraction equipment. It includes a reaction component and a filter box; the reaction component includes a reactor, an isolation plate is fixedly installed in the reactor, an isolation box is fixedly installed below the isolation plate, an adjustment tube is slidably connected to the isolation plate, and a first flow guide tube is rotatably connected to the adjustment tube; a connecting block is fixedly installed on the first flow guide tube, a support block is rotatably connected to the bottom of the isolation box, and the connecting block and the support block are plugged in and limited; a first flow guide hole is opened on the support block, and the first flow guide tube is plugged in and matched with the first flow guide hole, a plurality of connecting columns are fixedly installed at the bottom of the support block, and the filter box is fixedly installed on the connecting columns. The first flow guide tube of the present invention is lifted and rotated to control the connection between the mixing area of ​​the reactor and the filter box, and to control the plug-in limit of the connecting block and the support block, so that the filter box rotates with the first flow guide tube and filters the mixed liquid.

Description

Technical Field

[0001] The present invention relates to the technical field of polypeptide extraction equipment, and more specifically to a reaction device for preparing bone-derived polypeptides by fermenting bovine bones. Background Art

[0002] When extracting bone-derived peptides, the mixed solution of bovine bone powder is often enzymatically hydrolyzed by protease, and then the impurities in the peptide solution are initially filtered out by filtration. When the existing bone-derived peptide extraction equipment initially filters the peptide solution, the peptide solution naturally passes through the filter, and after enzymatic hydrolysis of the defatted bovine bone powder, there will still be a small amount of lipid viscous matter, which causes the filter to be blocked and affects the filtration efficiency. In view of this, we propose a reaction device for preparing bone-derived peptides by bovine bone fermentation. Summary of the invention

[0003] The purpose of the present invention is to provide a reaction device for preparing bone-derived polypeptides by fermenting bovine bones, which solves the technical problem in the prior art that the polypeptide liquid is easily blocked when passing through the filter screen and the filtering effect is not ideal.

[0004] The embodiment of the present invention provides a reaction device for preparing bone-derived polypeptides by fermenting bovine bones, comprising a reaction component and a filter box;

[0005] The reaction assembly comprises a reactor, an isolation plate is fixedly installed in the reactor, an isolation box is fixedly installed below the isolation plate, an adjustment tube is slidably connected to the isolation plate, a first flow guide tube is rotatably connected to the adjustment tube, a first limit block is fixedly installed on the isolation plate, a first limit groove is provided at the bottom of the first limit block, and the first flow guide tube is movably inserted in the first limit groove;

[0006] A connecting block is fixedly installed on the first guide tube, a supporting block is rotatably connected to the bottom of the isolation box, and the connecting block and the supporting block are plugged and limited; a first guide hole is opened on the supporting block, the first guide tube is plugged and matched with the first guide hole, a plurality of connecting columns are fixedly installed on the bottom of the support block, and the filter box is fixedly installed on the connecting columns;

[0007] The first guide pipe is lifted and rotated to control the connection between the mixing area of ​​the reactor and the filter box and to control the insertion and limiting of the connecting block and the supporting block, so that the filter box rotates with the first guide pipe and filters the mixed liquid.

[0008] Preferably, the support block is slidably connected to a plurality of limit columns, an elastic member is installed at the bottom of the limit column, one end of the elastic member is fixedly connected to the limit column, and the other end is fixedly connected to the support block, a plurality of limit holes are opened on the connecting block, and the limit columns are inserted into the limit holes for limiting the connecting block and the support block.

[0009] Preferably, it also includes a stirring assembly, which includes a motor, a hydraulic cylinder and a support shaft. The motor and the hydraulic cylinder are both fixedly mounted on the reactor, the output shaft of the motor is fixedly mounted with a sleeve, the output shaft of the hydraulic cylinder is fixedly connected to a support frame, the support frame is slidably connected to the reactor, the support frame is rotatably connected to the support shaft, the bottom of the support shaft passes through the first limit block and is fixedly connected to the first guide pipe, the support shaft is movably inserted in the reactor, a positioning column is fixedly mounted on the support shaft, the positioning column is slidably connected to the sleeve, and a spiral blade is fixedly mounted on the support shaft.

[0010] Preferably, a plurality of second flow guide pipes are slidably connected to the isolation plate, the second flow guide pipes are fixedly connected to the adjusting pipes through support rods, a plurality of second limit blocks are fixedly installed on the isolation plate, the second flow guide pipes are movably inserted in the second limit blocks, the second flow guide pipes are slidably connected to the isolation box, and the bottom of the second flow guide pipes passes through the isolation box and is connected to the filter box.

[0011] Preferably, the filter box comprises a bottom plate and a plurality of arc-shaped filter plates, and the bottom plate is fixedly mounted on the bottom of the connecting column.

[0012] A support tube is fixedly installed in the reactor, the bottom plate is rotatably connected to the support tube, two adjacent arc filter plates are plugged end to end, a plurality of arc filter plates are spliced ​​into a filter ring, the outlet ends of a plurality of second guide pipes are located on the inner side of the filter ring, a fixed plate is fixedly installed at the bottom of the arc filter plate, and the fixed plate is fixedly installed on the bottom plate.

[0013] Preferably, each arc-shaped filter plate is provided with a positioning hole;

[0014] The bottom of the isolation box is rotatably connected with a support ring, and the bottom of the support ring is fixedly installed with a plurality of positioning rods, which are plug-fitted with the positioning holes.

[0015] Preferably, a connecting rod is fixedly installed in the first flow guiding pipe, the outer diameter of the connecting rod is smaller than the inner diameter of the first flow guiding pipe, and a blocking block is fixedly installed at the bottom of the connecting rod;

[0016] The bottom plate is provided with a third flow guide hole, the blocking block is plugged into the third flow guide hole, the third flow guide hole is located directly above the support tube, and the support tube is provided with a plurality of second flow guide holes, which are used to connect the reactor with the inside of the support tube.

[0017] Preferably, an inspection plate is provided at the bottom of the reactor, and the inspection plate is located directly below an arc-shaped filter plate;

[0018] The bottom of the reactor is connected with a liquid outlet pipe, which is connected with the inside of the support pipe. A first control valve is installed on the liquid outlet pipe. The first control valve has an inlet and two outlets. The liquid outlet pipe is connected with the inlet of the first control valve, one outlet of the first control valve is connected with the slag discharge pipe, and the other outlet of the first control valve is connected with the feed port of the centrifuge.

[0019] Preferably, it also includes an enzyme storage tank, a liquid storage tank and a centrifuge, the enzyme storage tank is connected to the mixing zone of the reactor through a feeding pipe, and is used to provide protease to the reactor, and the liquid storage tank is connected to the mixing zone of the reactor, and is used to transport ultrapure water into the reactor;

[0020] A feeding pipe is connected to the reactor and connected to the cattle bone powder storage tank; a heating rod and a temperature sensor are installed in the reactor.

[0021] Preferably, a cleaning assembly is further included, the cleaning assembly including a liquid supply box, a first liquid supply pipe, a second liquid supply pipe, a third liquid supply pipe and a third control valve, one end of the first liquid supply pipe is connected to the liquid supply box, the other end of the first liquid supply pipe is connected to the inlet of the third control valve, the second liquid supply pipe and the third liquid supply pipe are respectively connected to two outlets of the third control valve, the second liquid supply pipe is connected to and installed with a fourth liquid supply pipe, the fourth liquid supply pipe is fixedly installed on the top of the reactor, the fourth liquid supply pipe is connected to and installed with a plurality of first nozzles, the first nozzles are inserted into the reactor and are used to flush the mixing zone, and the third liquid supply pipe is connected to and installed with a fifth liquid supply pipe;

[0022] A sealing plate is fixedly installed on the isolation box, a fifth liquid supply pipe is installed on the sealing plate, a plurality of second nozzles are connected and installed on the fifth liquid supply pipe, and the second nozzles are located outside the plurality of arc filter plates for backwashing the outer walls of the arc filter plates.

[0023] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0024] 1. The stirring assembly of the present invention controls the reaction device to enter multiple working modes. The stirring assembly can control the sufficient mixing and stirring of the protease and the bovine bone powder in the stirring zone of the reactor to improve the enzymatic hydrolysis efficiency. During the mixing and stirring, the tops of the first guide tube and the second guide tube are both in a blocked state and are not connected to the mixing zone of the reactor. After the enzymatic hydrolysis is completed, the support shaft of the stirring assembly can drive the first guide tube to move downward, control the mixing zone of the reactor and the filter box to be connected through the first guide tube, so that the polypeptide liquid enters the filter box from the mixing zone, and when the first guide tube moves downward, the connecting block and the supporting block are inserted and limited to each other. At this time, when the stirring drives the support shaft to rotate, the filter box will follow the rotation, thereby providing a centrifugal force for the polypeptide liquid, accelerating the polypeptide liquid to pass through the arc filter plate, and improving the filtration efficiency.

[0025] 2. When filtering the polypeptide liquid, the first guide tube moves downward and also drives the block to move downward and insert into the third guide hole, so as to prevent the polypeptide liquid flowing down from the first guide tube from being directly discharged from the third guide hole, thereby ensuring the reliability of filtration.

[0026] 3. The cleaning component of the present invention can respectively wash the mixing area of ​​the reactor and backwash the outer wall of the arc-shaped filter plate, and can effectively improve the cleaning effect by coordinating the rotation of the stirring component and the filter box.

[0027] 4. The present invention provides a plurality of arc filter plates which are plugged and assembled end to end. After the inspection panel is opened, the arc filter plates can be removed and replaced one by one, which effectively reduces the difficulty of maintaining the arc filter plates. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a schematic diagram of the overall structure of a reaction device for preparing bone-derived polypeptides by fermenting bovine bones, disclosed in a preferred embodiment of the present invention;

[0029] Figure 2 A schematic diagram of the reaction assembly structure of a reaction device for preparing bone-derived polypeptides by fermenting bovine bones disclosed in a preferred embodiment of the present invention;

[0030] Figure 3 A schematic diagram of the connection structure of the first flow guide pipe of a reaction device for preparing bone-derived polypeptides by fermentation of bovine bones disclosed in a preferred embodiment of the present invention;

[0031] Figure 4 A reaction device for preparing bone-derived polypeptides by fermenting cattle bones is disclosed in a preferred embodiment of the present invention. Figure 2 The enlarged structural diagram at A in the middle;

[0032] Figure 5 A partial cross-sectional view of a reaction device for preparing bone-derived polypeptides by fermenting bovine bones disclosed in a preferred embodiment of the present invention;

[0033] Figure 6 A reaction device for preparing bone-derived polypeptides by fermenting cattle bones is disclosed in a preferred embodiment of the present invention. Figure 2 The enlarged structural diagram at B in the middle;

[0034] Figure 7 A schematic diagram of a part of the structure of a reaction device for preparing bone-derived polypeptides by fermenting bovine bones disclosed in a preferred embodiment of the present invention;

[0035] Figure 8 This is a schematic diagram of the connection structure of a filter box of a reaction device for preparing bone-derived polypeptides by fermenting bovine bones, disclosed in a preferred embodiment of the present invention.

[0036] Explanation of the numbers in the figure: 1. reaction component; 2. stirring component; 3. filter box; 4. enzyme storage tank; 5. centrifuge; 6. heating rod; 7. cleaning component; 11. reactor; 12. isolation plate; 13. isolation box; 14. regulating pipe; 15. first guide pipe; 16. first limit block; 17. first limit groove; 18. connecting block; 19. support block; 21. motor; 22. hydraulic cylinder; 23. support shaft; 24. sleeve; 25. support frame; 26. positioning column; 27. spiral blade; 31. bottom plate; 32. arc filter plate; 33. positioning hole; 34. third guide hole; 35. fixing plate; 41. feeding pipe; 42. second control valve; 71. liquid supply box; 72. First liquid supply pipe; 73. Second liquid supply pipe; 74. Third liquid supply pipe; 75. Third control valve; 76. Fourth liquid supply pipe; 77. First nozzle; 78. Fifth liquid supply pipe; 79. Second nozzle; 110. First flow guide hole; 111. Limit column; 112. Elastic member; 113. Limit hole; 114. Connecting column; 115. Second flow guide pipe; 116. Support rod; 117. Second limit block; 118. Support ring; 119. Connecting rod; 120. Block; 121. Support pipe; 122. Second flow guide hole; 123. First control valve; 124. Guide rod; 125. Feed pipe; 126. Inspection plate; 127. Positioning rod; 128. Closing plate. DETAILED DESCRIPTION

[0037] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0038] Reference Figures 1 to 5 The present invention discloses a reaction device for preparing bone-derived polypeptides by fermenting bovine bones, comprising a reaction assembly 1, wherein the reaction assembly 1 comprises a reactor 11, wherein an isolation plate 12 is fixedly installed in the reactor 11, wherein the upper surface of the isolation plate 12 is inclined, and along the radial direction, the upper surface height of the isolation plate 12 gradually decreases from the outer circle to the center of the circle, so as to facilitate the subsequent outflow of the mixed liquid, wherein an isolation box 13 is fixedly installed below the isolation plate 12, wherein an adjusting tube 14 is slidably connected to the isolation plate 12, wherein a first flow guide tube 15 is rotatably connected to the adjusting tube 14, wherein the axis of the first flow guide tube 15 is colinear with the axis of the reactor 11, wherein a first limiting block 16 is fixedly installed on the isolation plate 12, wherein the first limiting block 16 is located directly above the first flow guide tube 15, wherein a first limiting groove 17 is provided at the bottom of the first limiting block 16, wherein the first flow guide tube 15 is movably inserted into the first limiting groove 17.

[0039] Reference Figure 4A connecting block 18 is fixedly installed on the first flow guide pipe 15, and a support block 19 is rotatably connected to the bottom of the isolation box 13. A first flow guide hole 110 is opened on the support block 19, and the first flow guide pipe 15 is plugged and matched with the first flow guide hole 110. A plurality of limiting columns 111 are slidably connected to the support block 19, and an elastic member 112 is installed at the bottom of the limiting column 111. One end of the elastic member 112 is fixedly connected to the limiting column 111, and the other end is fixedly connected to the support block 19. A plurality of limiting holes 113 are opened on the connecting block 18, and the limiting column 111 is inserted into the limiting hole 113, so that the support block 19 rotates with the connecting block 18 and the first flow guide pipe 15, and a plurality of connecting columns 114 are fixedly installed at the bottom of the support block 19.

[0040] Reference Figures 1 to 7 A plurality of second flow guide tubes 115 are slidably connected to the isolation plate 12, and the second flow guide tubes 115 are fixedly connected to the regulating tube 14 through a support rod 116. A plurality of second limit blocks 117 are fixedly installed on the isolation plate 12, and the second limit blocks 117 are located directly above the second flow guide tubes 115. The second flow guide tubes 115 are movably inserted into the second limit blocks 117, thereby blocking the top of the second flow guide tubes 115. The second flow guide tubes 115 are slidably connected to the isolation box 13, and the bottom of the second flow guide tubes 115 passes through the isolation box 13. The bottom of the isolation box 13 is rotatably connected with a support ring 118, and a connecting rod 119 is fixedly installed in the first flow guide tube 15. The outer diameter of the connecting rod 119 is smaller than the inner diameter of the first guide tube 15. A blocking block 120 is fixedly installed at the bottom of the connecting rod 119. A support tube 121 is fixedly installed in the reactor 11. A plurality of second guide holes 122 are opened on the support tube 121. The second guide holes 122 are used to connect the reactor 11 and the interior of the support tube 121. A liquid outlet pipe is arranged at the bottom of the reactor 11. The liquid outlet pipe is connected to the interior of the support tube 121. A first control valve 123 is installed on the liquid outlet pipe. The first control valve 123 has one inlet and two outlets. The liquid outlet pipe is connected to the inlet of the first control valve 123. One outlet of the first control valve 123 is connected to the slag discharge pipe.

[0041] A guide rod 124 is fixedly installed on the reactor 11, and a feed pipe 125 is installed on the top of the reactor 11. The feed pipe 125 is connected to the defatted cattle bone meal storage tank and is used for feeding cattle bone meal. An inspection plate 126 is provided at the bottom of the reactor 11, a plurality of positioning rods 127 are fixedly installed at the bottom of the support ring 118, and a sealing plate 128 is fixedly installed on the isolation box 13.

[0042] Reference Figures 1 to 3 and Figure 7The reaction device also includes a stirring assembly 2, which includes a motor 21, a hydraulic cylinder 22 and a support shaft 23. The motor 21 and the hydraulic cylinder 22 are both fixedly mounted on the reactor 11. The output shaft of the motor 21 is fixedly mounted with a sleeve 24. The output shaft of the hydraulic cylinder 22 is fixedly connected with a support frame 25. The support frame 25 is slidably connected to the guide rod 124. The support frame 25 is rotatably connected to the support shaft 23. The bottom of the support shaft 23 passes through the first limit block 16 and is fixedly connected to the first guide tube 15. The support shaft 23 is movably inserted in the reactor 11. A positioning column 26 is fixedly mounted on the support shaft 23. The positioning column 26 is slidably connected to the sleeve 24. A spiral blade 27 is fixedly mounted on the support shaft 23. The output shaft of the motor 21 drives the sleeve 24, the positioning column 26 and the support shaft 23 to rotate, thereby driving the first guide tube 15 to rotate. The output end of the hydraulic cylinder 22 controls the height of the support shaft 23 by pushing the lifting of the support frame 25, thereby adjusting the lifting of the first guide tube 15.

[0043] Reference Figures 2 to 5 and Figure 8 The reaction device also includes a filter box 3, which includes a bottom plate 31 and a plurality of arc-shaped filter plates 32. The upper surface of the bottom plate 31 is inclined, and along the radial direction, the upper surface height of the isolation plate 12 gradually decreases from the outer circle to the center of the circle. The bottom plate 31 is fixedly installed at the bottom of the connecting column 114, and the bottom plate 31 is rotatably connected to the support tube 121. Two adjacent arc-shaped filter plates 32 are spliced ​​end to end to form a filter ring. The outlet ends of the plurality of second guide pipes 115 are located on the inner side of the filter ring. A positioning hole 33 is opened on each arc-shaped filter plate 32, and the positioning rod 127 is plugged and matched with the positioning hole 33. The bottom plate 31 is provided with a third flow guide hole 34, the blocking block 120 is plugged into and matched with the third flow guide hole 34, the third flow guide hole 34 is located directly above the support tube 121, a fixing plate 35 is fixedly installed at the bottom of the arc-shaped filter plate 32, and the fixing plate 35 is fixedly installed on the bottom plate 31 with bolts, thereby ensuring the stability of the arc-shaped filter plate 32, and the inspection plate 126 is located directly below an arc-shaped filter plate 32. After the inspection plate 126 is disassembled, the bolts of the fixing plate 35 are disassembled, and the arc-shaped filter plate 32 can be disassembled and replaced, thereby reducing the difficulty of maintaining the arc-shaped filter plate 32.

[0044] The reaction device also includes an enzyme storage tank 4, a liquid storage tank and a centrifuge 5. The enzyme storage tank 4 is connected to the mixing zone of the reactor 11 through a feeding pipe 41, and is used to provide protease to the reactor 11. A second control valve 42 is installed on the feeding pipe 41. The liquid storage tank is connected to the mixing zone of the reactor 11, and is used to provide ultrapure water mixed with cattle bone powder to the reactor 11. The liquid inlet of the centrifuge 5 is connected to an outlet of the first control valve 123. The filtered polypeptide liquid is controlled by the first control valve 123 to enter the centrifuge 5 for centrifugal separation, thereby obtaining a purer polypeptide liquid.

[0045] A heating rod 6 and a temperature sensor are installed in the reactor 11. The heating rod 6 is located above the isolation plate 12 and is used to adjust the temperature of the protease hydrolysis process of the bovine bone protein, thereby ensuring the stability of the enzymatic hydrolysis process. The temperature sensor is installed in the reactor 11 and is at the top of the reactor 11. It is used to detect the temperature in the reactor 11, thereby adjusting the temperature in real time.

[0046] Reference Figure 1 and Figure 2 The reaction device also includes a cleaning component 7, which includes a liquid supply box 71, a first liquid supply pipe 72, a second liquid supply pipe 73, a third liquid supply pipe 74 and a third control valve 75. One end of the first liquid supply pipe 72 is connected to the liquid supply box 71, and the other end is connected to the inlet of the third control valve 75. The second liquid supply pipe 73 and the third liquid supply pipe 74 are respectively connected to the two outlets of the third control valve 75. The second liquid supply pipe 73 is connected and installed with a fourth liquid supply pipe 76, which is fixedly installed on the top of the reactor 11. The fourth liquid supply pipe 76 is connected and installed with a plurality of first nozzles 77. The first nozzles 77 are inserted into the reactor 11 and are used to flush the mixing zone. The third liquid supply pipe 74 is connected and installed with a fifth liquid supply pipe 78, which is installed on the sealing plate 128. The fifth liquid supply pipe 78 is connected and installed with a plurality of second nozzles 79, which are located on the outer sides of the plurality of arc-shaped filter plates 32 and are used to backwash the outer walls of the arc-shaped filter plates 32.

[0047] Working principle: the first guide tube 15 is inserted into the first limiting groove 17, and the second guide tube 115 is inserted into the second limiting block 117. At this time, the first guide tube 15 is located on the upper side of the first guide hole 110 and is not in contact with the support block 19. The defatted bovine bone powder, an appropriate amount of ultrapure water and protease are heated in the reactor 11. The output shaft of the motor 21 drives the sleeve 24, the positioning column 26, the support shaft 23 and the spiral blade 27 to rotate, and the mixed liquid is stirred to fully mix the protease and the bovine bone powder solution. The heating rod 6 heats the mixed liquid.

[0048] After the set enzymolysis time, the output shaft of the hydraulic cylinder 22 drives the support frame 25 and the support shaft 23 to descend, so that the first guide tube 15 descends and disengages from the first limiting groove 17 and communicates with the interior of the reactor 11, the bottom of the first guide tube 15 is inserted into the first guide hole 110, the second guide tube 115 is disengaged from the second limiting block 117 and communicates with the interior of the reactor 11, and the blocking block 120 is inserted into the third guide hole 34 to seal it.

[0049] The mixed liquid after enzymolysis enters the filter box 3 from the first guide tube 15 and the second guide tube 115. As the first guide tube 15 drives the connecting block 18 to descend, the limiting column 111 is inserted into the limiting hole 113. When the first guide tube 15 rotates following the output shaft of the motor 21, the limiting column 111 drives the supporting block 19, the connecting column 114 and the filter box 3 to rotate. Under the action of centrifugal force, the polypeptide liquid passes through the arc filter plate 32 and enters the centrifuge 5 from the liquid outlet at the bottom of the reactor 11 for the next centrifugation. The larger particles in the mixed liquid are deposited on the inner side of the arc filter plate 32, thereby realizing solid-liquid separation in the mixed liquid and effectively improving the separation efficiency.

[0050] The first control valve 123 switches direction so that the liquid outlet pipe of the reactor 11 is connected to the slag discharge pipe, and the output shaft of the hydraulic cylinder 22 drives the support shaft 23, the first guide pipe 15, the connecting rod 119 and the block 120 to continue to move downward, and the block 120 moves to the bottom of the third guide hole 34, and the third guide hole 34 is opened. The third control valve 75 controls the flow direction of the cleaning liquid, so that the first nozzle 77 flushes the inner wall of the reactor 11, and the second nozzle 79 backwashes the outer wall of the arc filter plate 32. The flushing liquid flows into the slag discharge pipe through the third guide hole 34, the support pipe 121, and the liquid outlet pipe of the reactor 11, and then flows out to realize the slag discharge process.

[0051] In order to improve the cleaning effect of the mixing zone of the reactor 11, the first guide pipe 15 can also be inserted into the first limiting groove 17, and the second guide pipe 115 can be inserted into the second limiting block 117 to isolate the mixing zone of the reactor 11. The continuous rotation of the spiral blade 27 can improve the cleaning effect. The mixed liquid after cleaning the mixing zone of the reactor 11 can be directly discharged through the first guide pipe 15, the third guide hole 34 and the support pipe 121, thereby reducing the impact of residues or lipid substances on the arc filter plate 32.

[0052] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A reaction device for preparing bone-derived polypeptides by fermentation of bovine bones, characterized in that: It comprises a reaction component (1) and a filter box (3); The reaction assembly (1) comprises a reaction kettle (11), an isolation plate (12) is fixedly installed in the reaction kettle (11), an isolation box (13) is fixedly installed below the isolation plate (12), an adjustment tube (14) is slidably connected to the isolation plate (12), a first flow guide tube (15) is rotatably connected to the adjustment tube (14), a first limit block (16) is fixedly installed on the isolation plate (12), a first limit groove (17) is provided at the bottom of the first limit block (16), and the first flow guide tube (15) is movably inserted into the first limit groove (17); A connecting block (18) is fixedly mounted on the first flow guide tube (15); a supporting block (19) is rotatably connected to the bottom of the isolation box (13); the connecting block (18) and the supporting block (19) are plugged and limited; a first flow guide hole (110) is formed on the supporting block (19); the first flow guide tube (15) is plugged and matched with the first flow guide hole (110); a plurality of connecting columns (114) are fixedly mounted on the bottom of the supporting block (19); and the filter box (3) is fixedly mounted on the connecting columns (114); The first flow guide pipe (15) is raised and lowered and rotated to control the connection between the mixing area of ​​the reaction kettle (11) and the filter box (3) and to control the plugging and limiting of the connection block (18) and the support block (19) so that the filter box (3) rotates along with the first flow guide pipe (15) and filters the mixed liquid; The stirring assembly (2) further comprises a stirring assembly (2), the stirring assembly (2) comprising a motor (21), a hydraulic cylinder (22) and a support shaft (23), the motor (21) and the hydraulic cylinder (22) being fixedly mounted on the reactor (11), the output shaft of the motor (21) being fixedly mounted with a sleeve (24), the output shaft of the hydraulic cylinder (22) being fixedly connected with a support frame (25), the support frame (25) being slidably connected to the reactor (11), the support frame (25) being rotatably connected to the support shaft (23), the bottom of the support shaft (23) passing through a first limit block (16) and being fixedly connected to a first flow guide pipe (15), the support shaft (23) being movably plugged into the reactor (11), a positioning column (26) being fixedly mounted on the support shaft (23), the positioning column (26) being slidably connected to the sleeve (24), and a spiral blade (27) being fixedly mounted on the support shaft (23); The support block (19) is slidably connected to a plurality of limiting columns (111), an elastic member (112) is installed at the bottom of the limiting column (111), one end of the elastic member (112) is fixedly connected to the limiting column (111), and the other end is fixedly connected to the support block (19), a plurality of limiting holes (113) are opened on the connection block (18), and the limiting columns (111) are inserted into the limiting holes (113) for limiting the connection block (18) and the support block (19); A plurality of second flow guide pipes (115) are slidably connected to the isolation plate (12); the second flow guide pipes (115) are fixedly connected to the regulating pipe (14) via a support rod (116); a plurality of second limit blocks (117) are fixedly mounted on the isolation plate (12); the second flow guide pipes (115) are movably inserted into the second limit blocks (117); the second flow guide pipes (115) are slidably connected to the isolation box (13); and the bottom of the second flow guide pipe (115) passes through the isolation box (13) and is in communication with the filter box (3); A connecting rod (119) is fixedly installed in the first flow guide pipe (15), and a blocking block (120) is fixedly installed at the bottom of the connecting rod (119); The filter box (3) comprises a bottom plate (31) and a plurality of arc-shaped filter plates (32); a third flow guide hole (34) is provided on the bottom plate (31); the blocking block (120) is plugged into and matched with the third flow guide hole (34); the third flow guide hole (34) is located directly above the support tube (121); a plurality of second flow guide holes (122) are provided on the support tube (121); the second flow guide holes (122) are used to connect the reaction kettle (11) and the interior of the support tube (121); It also includes an enzyme storage tank (4), a liquid storage tank and a centrifuge (5); the enzyme storage tank (4) is connected to the mixing zone of the reactor (11) through a feeding pipe (41) and is used to provide protease to the reactor (11); the liquid storage tank is connected to the mixing zone of the reactor (11) and is used to transport ultrapure water into the reactor (11); The reactor (11) is connected to a feed pipe (125), which is connected to a cattle bone powder storage tank; The cleaning assembly (7) further comprises a cleaning assembly (7), the cleaning assembly (7) comprising a liquid supply box (71), a first liquid supply pipe (72), a second liquid supply pipe (73), a third liquid supply pipe (74) and a third control valve (75), one end of the first liquid supply pipe (72) being in communication with the liquid supply box (71), the other end of the first liquid supply pipe (72) being in communication with the inlet of the third control valve (75), the second liquid supply pipe (73) and the third liquid supply pipe (74) being in communication with two outlets of the third control valve (75), the second liquid supply pipe (73) being in communication with a fourth liquid supply pipe (76) being fixedly mounted on the top of the reaction kettle (11), the fourth liquid supply pipe (76) being in communication with a plurality of first spray heads (77) being inserted into the reaction kettle (11) and used for washing the mixing zone, and the third liquid supply pipe (74) being in communication with a fifth liquid supply pipe (78); A plurality of second nozzles (79) are connected and installed on the fifth liquid supply pipe (78) for backwashing the outer wall of the arc-shaped filter plate (32).

2. The reaction device for preparing bone-derived polypeptides by fermentation of bovine bones according to claim 1, characterized in that: The bottom plate (31) is fixedly mounted on the bottom of the connecting column (114); A support tube (121) is fixedly installed in the reaction kettle (11), the bottom plate (31) is rotatably connected to the support tube (121), two adjacent arc-shaped filter plates (32) are plugged end to end, a plurality of arc-shaped filter plates (32) are spliced ​​into a filter ring, the outlet ends of a plurality of second flow guide tubes (115) are located inside the filter ring, a fixed plate (35) is fixedly installed at the bottom of the arc-shaped filter plate (32), and the fixed plate (35) is fixedly installed on the bottom plate (31).

3. The reaction device for preparing bone-derived polypeptides by fermentation of bovine bones according to claim 2, characterized in that: Each arc-shaped filter plate (32) is provided with a positioning hole (33); The bottom of the isolation box (13) is rotatably connected to a support ring (118), and a plurality of positioning rods (127) are fixedly installed on the bottom of the support ring (118), and the positioning rods (127) are plug-fitted into the positioning holes (33).

4. The reaction device for preparing bone-derived polypeptides by fermentation of bovine bones according to claim 2, characterized in that: The outer diameter of the connecting rod (119) is smaller than the inner diameter of the first flow guide tube (15).

5. The reaction device for preparing bone-derived polypeptides by fermentation of bovine bones according to claim 2, characterized in that: The bottom of the reaction kettle (11) is provided with an inspection plate (126), and the inspection plate (126) is located directly below an arc-shaped filter plate (32); The bottom of the reactor (11) is connected to a liquid outlet pipe, the liquid outlet pipe is connected to the inside of the support pipe (121), a first control valve (123) is installed on the liquid outlet pipe, the first control valve (123) has an inlet and two outlets, the liquid outlet pipe is connected to the inlet of the first control valve (123), one outlet of the first control valve (123) is connected to the slag discharge pipe, and the other outlet of the first control valve (123) is connected to the feed port of the centrifuge (5).

6. A reaction device for preparing bone-derived polypeptides by fermentation of bovine bones according to any one of claims 1 to 5, characterized in that: A heating rod (6) and a temperature sensor are installed in the reaction kettle (11).

7. The reaction device for preparing bone-derived polypeptides by fermentation of bovine bones according to claim 2, characterized in that: A sealing plate (128) is fixedly mounted on the isolation box (13), a fifth liquid supply pipe (78) is mounted on the sealing plate (128), and a second nozzle (79) is located outside the plurality of arc-shaped filter plates (32).

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