Pesticide composition for preventing and treating grubs

Abstract

The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and treating grubs. The invention relates to an insecticidal composition, which is prepared by compounding dichlorthiopyracil and rotenone, or is prepared by compounding dichlorthiopyracil and bifenthrin, and the insecticidal composition is prepared by compounding dichlorthiopyracil and rotenone, or is prepared by compounding dichlorthiopyracil and bifenthrin. The compound combination of the insecticidal composition shows a combined synergistic effect on grub 2-instar larvae, can improve the prevention and treatment effect on grubs, reduces the pesticide dosage and pesticide residues, realizes high efficiency, low residue and environmental protection of medicaments, and has important significance for developing efficient medicaments for preventing and treating grubs.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and controlling white grubs. Background Art

[0002] Grubs are a general term for the larvae of the Scarabaeidae family of the order Coleoptera. They are a group of underground pests with the most species, the widest distribution range, and the most serious damage. Larval grubs mainly feed on underground rhizomes and pods of a variety of crops, causing the crops to wither and die; while adult scarab beetles feed on leaves of crops, fruit trees, and forest trees, causing buds and leaves to break, seriously affecting the quality and yield of agricultural products. With the restriction and ban on the use of some highly toxic and high-residue pesticides, many current agents are not ideal for the prevention and control of grubs. Therefore, it is particularly important to develop highly effective agents for the prevention and control of grubs.

[0003] Dichlorothiopyrimidine (dicloromezotiaz) is a new type of mesoionic pyrimidone insecticide developed by DuPont (now Corteva) in the United States. Its CAS registration number is 1263629-39-5 and its structural formula is as follows:

[0004]

[0005] Unlike neonicotinoid insecticides, dichlorothiazide acts on nicotinic acetylcholine receptors and exerts insecticidal activity by blocking the neurotransmission of target pests, that is, a nicotinic acetylcholine receptor inhibitor. Dichlorothiazide has a novel chemical structure and mechanism of action, is highly effective, has low toxicity and is environmentally friendly. It has high activity against diamondback moth, armyworm and peach aphid, and can effectively control Lepidoptera and Hemiptera pests at low doses.

[0006] Although dichlorothiazide has the above advantages, as a chemical insecticide, if used alone for a long time, it will inevitably cause the generation and accumulation of pest resistance like other chemical insecticides, thereby reducing its control effect. Therefore, it is very necessary to screen the compound agent of dichlorothiazide.

[0007] The inventors screened and compounded dichlorothiazide with existing insecticidal active ingredients and found that when dichlorothiazide is compounded with rotenone or bifenthrin, it exhibits a synergistic effect on white grubs, can improve the control effect on white grubs, and can be used to develop highly effective agents for controlling underground pest white grubs.

[0008] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention and should not be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art. Summary of the invention

[0009] The object of the present invention is to provide a pesticide composition for controlling white grubs, which can reduce the risk of drug resistance of dichlorothiazide and protect dichlorothiazide to a certain extent; and at the same time, can improve the control effect on pests.

[0010] To achieve the above object, the present invention provides the following technical solutions:

[0011] Disclosed is an insecticidal composition, which is compounded by dichlorothiazide and rotenone, or compounded by dichlorothiazide and bifenthrin.

[0012] More specifically, the mass ratio of dichlorothiazide to rotenone is 1-18:24-1.

[0013] More specifically, the compounding mass ratio of dichlorothiazide to bifenthrin is 1-40:15-1.

[0014] The present invention also provides the use of the insecticide composition in preventing and controlling underground pests of crops or in preparing a pesticide for preventing and controlling underground pests of crops.

[0015] More specifically, the underground crop pests include white grubs.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] (1) The insecticide composition of the present invention is a binary compound of dichlorothiapyrim and rotenone or bifenthrin, which contains two active ingredients. Compared with the use of dichlorothiapyrim alone, it can reduce the risk of insecticide resistance in pests, delay the generation and development of insecticide resistance in pests, and protect dichlorothiapyrim to a certain extent.

[0018] (2) The compound combination of the insecticide composition of the present invention exhibits a combined synergistic effect on the second-instar larvae of white grubs, which can improve the control effect on white grubs, reduce the amount of pesticides used and pesticide residues, achieve high efficiency, low residue, and green environmental protection of the agent, which is of great significance for the development of high-efficiency agents for controlling white grubs. DETAILED DESCRIPTION

[0019] The technical solution of the present invention is clearly and completely described below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them.

[0020] 1. Materials and Methods

[0021] 1.1 Test pests

[0022] The copper green beetles were collected from the peanut field and raised in an artificial insect breeding room with fresh and clean elm leaves. The breeding conditions were: temperature 25±1℃, humidity 65±5%, photoperiod 16L / 8D. Eggs of the same age were selected for hatching, and the hatched larvae were fed with potato tubers. The 2nd instar larvae of the same size were selected as the test pests.

[0023] 1.2 Test agents

[0024] 95% dichlorothiazide TC (Hubei Guoyun Furui Technology Co., Ltd.), 94% rotenone TC (extracted from the roots of rotenone), 97% bifenthrin TC (Jiangsu Yangnong Chemical Co., Ltd.).

[0025] 1.3 Test method: (Refer to "NY / T 1154.6-2006 Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides Part 6: Insecticide Activity Test - Insect Immersion Method").

[0026] Preparation of the drug solution: Dissolve the original drug with dimethyl sulfoxide first, and then dilute with 0.05% TritonX-100 aqueous solution to prepare a single-dose stock solution; then set up multiple groups of proportions, and dilute each single dose and each group of mixed proportions with 0.05% TritonX-100 aqueous solution in equal proportions to 5 mass concentration gradients, each mass concentration drug solution 50mL, for standby use.

[0027] Liquid treatment: Immerse the test insects in the liquid for 20 seconds, then take them out, absorb the excess liquid with absorbent paper, put them into a finger-shaped tube containing potato tubers of the same size, and seal them with double gauze. Each treatment was repeated 4 times, with 20 insects immersed in each repetition, and the treatment with 0.05% TritonX-100 aqueous solution was set as a blank control.

[0028] The treated test insects were transferred to a room with a temperature of 25±1℃ and a humidity of 65±5% without light. The death of the test insects was investigated after 3 days. The insects that did not move when touched lightly with a brush were considered dead.

[0029] 2. Data processing

[0030] The total number of insects and the number of dead insects in each treatment were investigated, and the corrected mortality rate of each treatment was calculated. The DPS software was used to perform regression analysis on the logarithmic value of the concentration of each treatment agent and the corrected mortality probability value to calculate the LC of each treatment agent. 50 .

[0031]

[0032] In the above formula: P--mortality rate, unit is %; K--number of dead insects; N--total number of insects treated.

[0033]

[0034] In the above formula: P1--corrected mortality rate, unit is %; P t --Treatment mortality rate, in %; P0--blank control mortality rate, in %.

[0035] The co-toxicity coefficient (CTC value) of the mixture was calculated using the Sun Yunpei method, and the combined effect was divided according to the following criteria: a co-toxicity coefficient (CTC) ≥ 120 indicated a synergistic effect; a co-toxicity coefficient (CTC) ≤ 80 indicated an antagonistic effect; and a co-toxicity coefficient (CTC) < 80 indicated an additive effect.

[0036] 3. Results and Analysis

[0037] The results are shown in Tables 1-2.

[0038] Table 1 The combined effect of dichlorothiazide and rotenone on the 2nd instar larvae of white grubs

[0039]

[0040]

[0041] It can be seen from Table 1 that within the mass ratio of 1-18:24-1, the co-toxicity coefficient of the combination of dichlorothiazide and rotenone in killing the 2nd instar larvae of white grubs is greater than 120, that is, the combined action type of the combination of dichlorothiazide and rotenone in killing white grubs is a synergistic effect, which can improve the control effect on white grubs.

[0042] Table 2 The combined effect of dichlorothiazide and bifenthrin on the 2nd instar larvae of white grubs

[0043] Drug name and ratio <![CDATA[LC 50 (mg / L)]]> ATI TTI CTC Dichlorothiazide 2.847 100.000 -- -- Bifenthrin 5.159 55.185 -- -- Dichlorothiazide 1: Bifenthrin 15 4.054 70.227 57.986 121.110 Dichlorothiazide 1: Bifenthrin 7 1.271 223.997 60.787 368.495 Dichlorothiazide 1: Bifenthrin 3 3.168 89.867 66.389 135.365 Dichlorothiazide 1: Bifenthrin 1 2.977 95.633 77.593 123.250 Dichlorothiazide 3: Bifenthrin 1 1.495 190.435 88.796 214.463 Dichlorothiazide 7: Bifenthrin 1 0.637 446.939 94.398 473.461 Dichlorothiazide 15: Bifenthrin 1 0.928 306.789 97.199 315.629 Dichlorothiazide 30: Bifenthrin 1 1.873 152.002 98.554 154.232 Dichlorothiazide 40: Bifenthrin 1 2.272 125.308 98.907 126.693

[0044] It can be seen from Table 2 that within the mass ratio of 1-40:15-1, the co-toxicity coefficient of the combination of dichlorothiazide and bifenthrin in killing the 2nd instar larvae of white grubs is greater than 120, that is, the combined action type of the combination of dichlorothiazide and bifenthrin in killing white grubs is a synergistic effect, which can improve the control effect on white grubs.

[0045] In summary, the compound combination of the insecticidal composition of the present invention shows a combined synergistic effect on the second-instar larvae of white grubs, can improve the control effect on white grubs, reduce the amount of pesticides used and pesticide residues, achieve high efficiency, low residue, and green environmental protection of the agent, which is of great significance for the development of high-efficiency agents for controlling white grubs.

Claims

1. An insecticidal composition, characterized in that: The insecticide composition is prepared by compounding dichlorothiazide and rotenone, or by compounding dichlorothiazide and bifenthrin.

2. The insecticidal composition according to claim 1, characterized in that The compounding mass ratio of dichlorothiazide to rotenone is 1-18:24-1.

3. The insecticidal composition according to claim 1, characterized in that The compounding mass ratio of dichlorothiazide to bifenthrin is 1-40:15-1.

4. Use of the insecticidal composition according to any one of claims 1 to 3 in controlling underground pests of crops or in preparing an agent for controlling underground pests of crops.

5. The use according to claim 4, characterized in that: The underground pests of crops include white grubs.

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