High-quality lactase aqueous solution and preparation method thereof

Abstract

The invention provides a high-quality lactase aqueous solution and a preparation method thereof. The preparation method comprises the following steps: step (1), weighing lactase, water and glycerol according to a formula; the method comprises the following steps: firstly adding water into a container, then adding part of glycerol into the water, and stirring; (2) continuously stirring the mixed solution obtained in the step (1), and adding lactase while stirring; and (3) adding the rest glycerol while stirring, and standing after adding. According to the invention, the mixing is very uniform, no blistering or turbidity is generated, and the final product is very uniform and transparent in character; the lactase is added in a proper proportion of glycerol to water, so that infectious microbes cannot be generated, and the bacteriostatic effect is achieved; and meanwhile, in the production process, the time is shortened, the production efficiency is improved, and the probability of bacterial contamination is also reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of food, and in particular to a high-quality lactase aqueous solution and a preparation method thereof, belonging to the technical field of food preparation. Background Art

[0002] Lactose is a disaccharide composed of galactose and glucose connected by β-1,4-glycosidic bonds. About 70% of the world's population, especially Asians, suffer from "lactose intolerance", the main symptoms of which include bowel sounds, diarrhea, intestinal obstruction and flatulence. This is due to the lack of enzymes in the body that can degrade lactose, causing the lactose that enters the intestine to become an energy source for microbial flora, thereby triggering the production of lactic acid and carbon dioxide. Taking lactase orally or adding lactase to dairy products can effectively alleviate the above problems.

[0003] Lactase, also known as β-galactosidase (β-D-galactosedase, EC3.2.1.23), is an enzyme that can hydrolyze β-D galactoside and galactose, and also has a galactoside transfer function. Commercial lactase is mainly used in the treatment of lactose intolerance, processing of milk and whey, production of low-lactose milk and low-lactose dairy products, and preparation of oligosaccharides. In nature, lactase is widely found in the small intestine of young mammals, plants such as almonds and apricots, and microorganisms such as bacteria, yeasts and molds.

[0004] Lactose is the main carbohydrate in dairy products and the main energy source for infants. After lactose enters the body, it is broken down into glucose and galactose by the action of lactase in the small intestine. Galactose is essential for the development of infant brains and is closely related to the rapid growth of infant brains. In addition, lactic acid produced by lactose fermentation in the intestine can enhance the absorption and utilization of minerals such as calcium, phosphorus, potassium, and iron in food.

[0005] Lactase deficiency refers to the low activity of the membrane-bound enzyme lactase in the chorionic cells of the brush border of the small intestine. Lactase deficiency can be divided into three categories: congenital lactase deficiency, secondary lactase deficiency and primary lactase deficiency (also known as adult lactase deficiency). The latter is the most common and has the highest incidence.

[0006] Although lactase deficiency is considered normal and is usually related to inherited gene mutations caused by different dietary habits formed over generations, the timing of its occurrence varies by race and region.

[0007] Untreated lactose may lead to lactose maldigestion or lactose malabsorption due to the lack of lactase. When the undecomposed and absorbed lactose enters the colon, it is fermented by intestinal bacteria into small molecular organic acids and produces some gases, most of which can be reabsorbed by the colon. Unabsorbed or still undecomposed lactose can cause symptoms such as intestinal rumbling, abdominal distension, abdominal pain, flatulence, discomfort, diarrhea, and some people may also experience belching, nausea, etc. These symptoms are collectively referred to as lactose intolerance, and they vary greatly from person to person. Severe lactose intolerance often occurs within 30 minutes to several hours after ingesting a certain amount of lactose.

[0008] Lactose intolerance has a greater impact on infants and young children, often accompanied by symptoms such as diaper rash, vomiting, and growth retardation, and sometimes nausea in adults. Lactose intolerance can be divided into four types: primary, secondary, congenital, and developmental. The cause is related to multiple factors such as lactase activity in the small intestine, lactose intake, and whether other types of food are consumed at the same time.

[0009] At present, the common production method of lactase aqueous solution products is to directly mix water, glycerin and lactase, or use high-speed stirring during the stirring process, which results in a very long standing time for defoaming, or some foam cannot be eliminated at all, and the foam reduces the yield; such products are prone to produce lactase particles and are also easily contaminated by bacteria due to the long standing time. Summary of the invention

[0010] In order to solve the problems in the above-mentioned background technology, the present invention provides a lactase aqueous solution. Through the special mixing process of the present invention, the production efficiency is greatly improved, and a lactase aqueous solution with uniform quality and not easy to be contaminated by bacteria is prepared.

[0011] The specific technical solution is: a high-quality lactase aqueous solution is obtained by a preparation method, and the preparation method comprises the following steps.

[0012] In step (1), the amounts of lactase, glycerol and water are 4.1-5.2 parts, 6.7-30.6 parts and 1.0-4.5 parts respectively by weight.

[0013] Preferably, lactase, water and glycerol are weighed according to the formula; based on the total net weight of 14 g per bottle, the masses of lactase, glycerol and water in the formula are 4.76 g, 7.32 g and 1.92 g, respectively.

[0014] Water is first added to the container, then part of the glycerin is added to the water and stirred.

[0015] The weight of glycerol added in step (1) is 10-90% of the weight of water. The stirring conditions are: the speed is 80-120 rpm, and the stirring time is 5-15 minutes.

[0016] Step (2), the mixed solution obtained in step (1) is continuously stirred, and lactase is added while stirring.

[0017] The stirring condition is 20 to 200 revolutions per minute, and the adding process takes 1 to 3 hours. After all the lactase is poured in, there is no need to leave it standing.

[0018] Step (3), add the remaining glycerin while stirring, and let it stand after adding.

[0019] The stirring conditions in step (3) are: a rotation speed of 20 to 200 revolutions per minute, and the remaining glycerol is added in 0.5 to 1.5 hours. After the remaining glycerol is added, the mixture is left to stand for 12 to 48 hours.

[0020] The technical solution provided by the present invention has the following technical effects.

[0021] First, the mixing is very uniform, without any foaming or turbidity, and the final product is very uniform and transparent.

[0022] Second, bacteria are well controlled. Since glycerol has an antibacterial effect, adding lactase at a suitable ratio of glycerol to water will not produce any bacteria, thus achieving an antibacterial effect. At the same time, the adjusted ratio of glycerol to water is appropriate, so that lactase can be dissolved in water very evenly. If the ratio of glycerol to water is not within this range, lactase will be difficult to dissolve in the mixed solution, resulting in turbidity and easy bacterial contamination.

[0023] Third, due to the short standing time, short mixing time and antibacterial effect, the activity of lactase is guaranteed; at the same time, the time in the production process is shortened, the production efficiency is improved, and the chance of contamination is reduced. DETAILED DESCRIPTION

[0024] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] The present invention also provides embodiments as shown in the following table: Example 1

[0026] A high-quality lactase aqueous solution is obtained by a preparation method, and the preparation method comprises the following steps.

[0027] Step (1), weigh lactase, water and glycerol according to the formula; based on the total net weight of 14g per bottle, the masses of lactase, glycerol and water in the formula are: 4.76g, 7.32g, 1.92g respectively.

[0028] Water is first added into the container, and then some glycerin is added into the water and stirred; the weight of the added glycerin is 20% of the weight of the water. The stirring conditions are: the speed is 80 rpm and the stirring is for 30 minutes.

[0029] Step (2), the mixed solution obtained in step (1) is continuously stirred, and lactase is added while stirring; the stirring condition is 150 revolutions per minute, and the addition process is 2 hours. After all the lactase is poured in, there is no need to leave it.

[0030] Step (3), add the remaining glycerin while stirring at 100 rpm, and add the remaining glycerin over 1 hour. After adding the remaining glycerin, let it stand for 48 hours, at which time it becomes completely clear. Example 2

[0031] A high-quality lactase aqueous solution is obtained by a preparation method, and the preparation method comprises the following steps.

[0032] Step (1), weighing lactase, water and glycerol according to the formula; based on the total net weight of 14g per bottle, the masses of lactase, glycerol and water in the formula are: 5.12g, 6.84g, 2.04g respectively.

[0033] Water is first added to the container, and then some glycerin is added to the water and stirred; the weight of the added glycerin is 50% of the weight of the water. The stirring conditions are: the speed is 120 rpm, and the stirring is for 5 minutes.

[0034] Step (2), the mixed solution obtained in step (1) is continuously stirred, and lactase is added while stirring; the stirring condition is 50 revolutions per minute, and the addition process is 2.5 hours. After all the lactase is poured in, there is no need to leave it.

[0035] Step (3), add the remaining glycerin while stirring at a speed of 150 rpm, and add the remaining glycerin in 0.5 hours. After adding the remaining glycerin, let it stand for 24 hours, at which time it becomes completely clear. Example 3

[0036] A high-quality lactase aqueous solution is obtained by a preparation method, and the preparation method comprises the following steps.

[0037] Step (1), weighing lactase, water and glycerol according to the formula; based on the total net weight of 14g per bottle, the masses of lactase, glycerol and water in the formula are: 4.57g, 8.22g, 1.21g respectively.

[0038] Water is first added into the container, and then some glycerin is added into the water and stirred; the weight of the added glycerin is 60% of the weight of the water. The stirring conditions are: the speed is 80 rpm and the stirring is for 15 minutes.

[0039] Step (2), the mixed solution obtained in step (1) is continuously stirred, and lactase is added while stirring; the stirring condition is 50 revolutions per minute, and the addition process is 2 hours. After all the lactase is poured in, there is no need to leave it.

[0040] Step (3), add the remaining glycerin while stirring at 80 rpm, and add the remaining glycerin over 1 hour. After adding the remaining glycerin, let it stand for 48 hours, at which time it becomes completely clear. Example 4

[0041] A high-quality lactase aqueous solution is obtained by a preparation method, and the preparation method comprises the following steps.

[0042] Step (1), weighing lactase, water and glycerol according to the formula; based on the total net weight of 14g per bottle, the masses of lactase, glycerol and water in the formula are: 4.18g, 7.56g, 2.26g respectively.

[0043] Water is first added into the container, and then some glycerin is added into the water and stirred; the weight of the added glycerin is 80% of the weight of the water. The stirring conditions are: the speed is 100 rpm and the stirring is for 10 minutes.

[0044] Step (2), the mixed solution obtained in step (1) is continuously stirred, and lactase is added while stirring; the stirring condition is 20 revolutions per minute, and the addition process is 3 hours. After all the lactase is poured in, there is no need to leave it.

[0045] Step (3), add the remaining glycerin while stirring at 20 rpm, and add the remaining glycerin over 1.5 hours. After adding the remaining glycerin, let it stand for 12 hours, at which time it becomes completely clear.

[0046] The invention provides an antibacterial experiment of a lactase enzyme aqueous solution.

[0047] The currently common production method of lactase aqueous solution, i.e. simple mixing and stirring, will generate a large number of bubbles, which requires standing for more than 24 hours, usually 48 hours before complete defoaming and filling. The method of the product of the present invention can avoid the generation of bubbles, and at the same time, the antibacterial effect of glycerol is utilized, so that the shelf life can be extended without adding preservatives.

[0048] The ratios of Comparative Examples 1-3 are exactly the same as those of Examples 1-3 of the present invention, except that a conventional mixing method is used, namely, direct mixing and stirring, and standing to defoam.

[0049] The present embodiments 1-4 and comparative examples 1-3 were tested by using the drug sensitive paper method, and the testing process was as follows.

[0050] (1) Preparation of drug-sensitive paper strips.

[0051] Take the qualitative filter paper and use a hole puncher to punch it into a small round paper with a diameter of 6mm. Take 50 round paper pieces and put them into a clean and dry vial, and wrap them with a single layer of kraft paper. After high pressure sterilization at 15 pounds for 15-20 minutes, place them in a 37℃ incubator to dry. Based on the saturated water absorption of each paper piece of 0.01ml, add 0.5ml of the drug solution to the vial containing 50 paper pieces, turn it over from time to time, so that the filter paper can evenly absorb the drug solution, soak it for 30 minutes, and place it in a 37℃ incubator overnight. After drying, seal it and store it in a dark and dry place.

[0052] (2) Preparation of drug solution.

[0053] The lactase aqueous solution obtained in this example, Example 1-4 and Comparative Example 1-3, was diluted tenfold and soaked in drug-sensitive paper.

[0054] (3) Test operation methods.

[0055] After 15 minutes, place the plate with the paper attached in a 37°C incubator for 16-24 hours, and observe and record the results.

[0056] Result judgment criteria.

[0057] The number of infected colonies.

[0058] Colony count > 20 is prone to bacterial contamination.

[0059] 15-20 is easy to be infected with bacteria.

[0060] 10-14 Mildly susceptible to bacteria.

[0061] <10 is not easy to be contaminated with bacteria.

[0062] 0 antibacterial.

[0063] 4) Test results: See Table 1.

[0064] Table 1 Results of incubator contamination test project Example 1 Paper Example 2 Paper Example 3 Paper Example 4 Paper Comparative Example 1 Comparative Example 2 Comparative Example 3 Inhibition zone (mm) 5 1 2 3 19 17 18

[0065] Conclusion: It can be seen that the lactase aqueous solution of the present invention has a very stable shelf life without adding preservatives.

[0066] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for preparing a high-quality lactase aqueous solution, It is characterized in that The following steps are involved: Step (1), weighing lactase, water and glycerol according to the formula; first adding water into a container, then adding part of the glycerol into the water and stirring; Step (2), continuously stirring the mixed solution obtained in step (1), and adding lactase while stirring; Step (3), add the remaining glycerin while stirring, and let it stand after adding.

2. A method for preparing a high-quality lactase aqueous solution according to claim 1, Features: In terms of mass proportions, the proportions of lactase, glycerol and water are 4.1-5.2 parts, 6.7-30.6 parts and 1.0-4.5 parts respectively.

3. A method for preparing a high-quality lactase aqueous solution according to claim 1, Features: The weight of glycerol added in step (1) is 10-90% of the weight of water.

4. A method for preparing a high-quality lactase aqueous solution according to claim 1, Features: In step (1), the stirring conditions are: a rotation speed of 80 to 120 revolutions per minute, and stirring for 5 to 30 minutes.

5. The method for preparing a high-quality lactase aqueous solution according to claim 1, Features: The stirring condition in step (2) is 20 to 200 revolutions per minute, and the addition process takes 1 to 3 hours. After all the lactase is poured in, there is no need to leave it standing.

6. A method for preparing a high-quality lactase aqueous solution according to claim 1, Features: The stirring conditions in step (3) are: a rotation speed of 20 to 200 revolutions per minute, and the remaining glycerol is added in 0.5 to 1.5 hours.

7. The method for preparing a high-quality lactase aqueous solution according to claim 1, Features: After adding the remaining glycerol in step (3), let it stand for 12 to 48 hours.

8. A high-quality lactase aqueous solution prepared by the method for preparing a high-quality lactase aqueous solution according to any one of claims 1 to 7.