Peach blood stasis-dispersing decoction and preparation method thereof

Abstract

The invention discloses a peach and safflower stasis-dispersing decoction and a preparation method thereof. The peach kernel and safflower stasis-dispersing decoction comprises the following raw materials: 5-10 parts of safflower, 8-15 parts of angelica sinensis, 5-8 parts of peach kernel, 5-8 parts of ligusticum wallichii, 8-15 parts of poria cocos, 8-15 parts of ginseng, 8-15 parts of salvia miltiorrhiza, 5-8 parts of liquorice and 8-15 parts of radix achyranthis bidentatae. Compared with the prior art, the peach and safflower stasis-dispersing decoction prepared by the invention has the advantages of prevention and treatment of deep venous thrombosis, meridian warming and pain relieving, long shelf life and the like.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicines, and in particular to a Taohong Huayu decoction and a preparation method thereof. Background Art

[0002] Deep vein thrombosis refers to a variety of reasons that cause blood to abnormally coagulate in the venous cavity to form blood clots, resulting in lumen obstruction and hemodynamic changes, which can also affect blood circulation. Therefore, this disease is also considered an inflammatory non-suppurative disease. Once a thrombus breaks off, it may cause fatal embolism. At present, the clinical prevention of deep vein thrombosis mainly uses anticoagulants, which cannot eliminate the thrombus that has already formed, so they can only control the symptoms so that they do not worsen and are prone to adverse reactions.

[0003] CN111840525A discloses a composition for treating thromboangiitis and a preparation method thereof, and the effect of treating thromboangiitis is enhanced by adding QK fibrinolytic enzyme and Bacillus subtilis powder. The composition prepared by the method uses medicinal powder, and the effective ingredients may not be well absorbed in the human body. CN102389470A discloses a Chinese medicine composition for promoting blood circulation and unblocking collaterals, which is composed of salvia miltiorrhiza, leech, chuanxiong, earthworm, red peony root, centipede, safflower, scorpion, millettia reticulata, pangolin, angelica, astragalus, Achyranthes bidentata, lycopodiella cuneata, hawthorn, and dandelion to treat venous thrombosis. The composition prepared by the method may have a short shelf life. Summary of the invention

[0004] In view of the above-mentioned defects of the prior art, the object of the present invention is to provide a Taohong Huayu Decoction, which has the effect of preventing and treating deep vein thrombosis, has a long shelf life and has the characteristics of no side effects.

[0005] The detailed technical scheme of the present invention is as follows:

[0006] The invention provides a preparation method of Taohong Huayu Decoction. The method comprises the following steps of weighing 5-10 parts of safflower, 8-15 parts of angelica, 5-8 parts of peach kernel, 5-8 parts of chuanxiong, 8-15 parts of tuckahoe, 8-15 parts of ginseng, 8-15 parts of salvia miltiorrhiza, 5-8 parts of liquorice and 8-15 parts of achyranthes bidentata, drying and crushing the mixture, passing through a 100-200 mesh sieve, adding 600-1100 parts of distilled water, heating and refluxing at 100-105 DEG C for extraction for 1-3 times, each extraction time being 1-2h, combining the filtrate, adding 0.1-0.5 parts of stevioside, stirring evenly, filtering, filling and sterilizing to obtain Taohong Huayu Decoction.

[0007] Among them, Carthami Flos, also known as red and blue flower, thorny safflower, and grass safflower, has the effects of mildly stimulating the heart, reducing coronary resistance, increasing coronary flow and myocardial nutritional blood flow; resisting myocardial ischemia, myocardial infarction, and arrhythmia; improving peripheral microcirculation disorders; anti-coagulation; lowering blood lipids and stimulating the uterus.

[0008] Angelicae Sinensis Radix, also known as Qin Gui, Yun Gui, Western Dang Gui, Gan Gui, Ma Wei Gui, and Min Dang Gui, has the effects and functions of reducing platelet aggregation and anti-thrombosis; promoting the function of the hematopoietic system; lowering blood lipids and anti-atherosclerosis; and has a bidirectional effect of excitation and inhibition on the uterus.

[0009] Persicae Semen, also known as hairy peach kernel, almond kernel, and big peach kernel; flat and long oval, 1.2-1.8 cm long, 0.8-1.2 cm wide, 0.2-0.4 cm thick. It has anti-coagulation, anti-thrombosis, blood flow improvement, anti-inflammatory, analgesic and anti-allergic effects. It can also relieve cough and asthma, and has a certain effect on tuberculosis.

[0010] Chuanxiong Rhizoma, also known as Shanjuqiong, Xiongjing, Xiangguo, Hujing, Maxian, Xixiong, Guanxiong; has effects on the cardiovascular and cerebrovascular systems and has an inhibitory effect on the heart; can dilate blood vessels and lower blood pressure; increase coronary flow; has a protective effect on myocardial and reperfusion injury; improves microcirculation; and improves cerebral circulation and cerebral ischemia.

[0011] Poria, also known as Fuling, Yunling, Futu, Songyu, Songshu, Songling, can prevent gastric ulcers, prevent and treat liver damage, have anti-cancer effects, and can accelerate heart rate. Pharmacological experiments have shown that it has diuretic and antibacterial effects and can improve the body's immune ability. In addition, it can also reduce blood sugar, reduce gastric acid, and enhance the contraction of isolated hearts.

[0012] Ginseng Radix Et Rhizoma, also known as Shencao, Jilin Ginseng, Bangchui, Earth Essence, Yellow Ginseng, Blood Ginseng, Earth Essence, Jinjing Yulan, and Baby Ginseng, has the effects and functions of bidirectional regulation of the central nervous system, promoting intelligence, relieving pain, relieving fever, resisting shock and weakening muscles. It has the effects of strengthening the heart, resisting ischemia, dilating blood vessels, and lowering blood pressure on the cardiovascular system; it protects and stimulates the hematopoietic function of the blood system, and has anti-coagulation and anti-thrombotic effects.

[0013] Salviae Miltiorrhizae, Radix Et Rhizoma, also known as red ginseng, blood root, Dahongpao, wood sheep milk, and mountain ginseng, has the functions of protecting the heart and brain system, lowering blood lipids, resisting atherosclerosis, promoting blood circulation and removing blood stasis, and has sex hormone-like effects.

[0014] Licorice Radix Rhizoma Glycyrrhizae, also known as honey sweet licorice, sweet licorice, Guolao, beautiful grass, honey grass, black root grass, Lingtong, Lingtong, sweet grass, powder grass, sweet root, and stick grass; it has adrenal cortex hormone effects, prevents arteriosclerosis, resists cerebral ischemia, and prevents diabetic complications.

[0015] Achyranthes bidentata Cyathulae Radix, also known as sweet Achyranthes bidentata, crooked Achyranthes bidentata, Tianquan Achyranthes bidentata, and domestic Achyranthes bidentata; animal experiments on Achyranthes bidentata have shown that its decoction and fluid extract can cause the uterus of experimental animals to contract strongly, and have anti-fertility effects and certain blood-activating effects.

[0016] Bacillus coagulans is a Gram-positive bacterium belonging to the Firmicutes phylum. Bacillus coagulans belongs to the genus Bacillus in taxonomy. The cells are rod-shaped, Gram-positive bacteria, with terminal spores and no flagella. It decomposes sugars to produce L-lactic acid and is a homolactic fermentation bacterium. The optimal growth temperature is 45-50℃, and the optimal pH is 6.6-7.0. Bacillus coagulans can utilize the decomposition of microorganisms under suitable conditions to convert raw materials into products that are easier to be absorbed and utilized by the human body through specific metabolic pathways in the fermentation of traditional Chinese medicine; Bacillus coagulans can use the active ingredients in traditional Chinese medicine as precursors through the metabolism of microorganisms to form new compounds; secondly, Bacillus coagulans can also produce abundant secondary metabolites during the growth process, and some secondary metabolites themselves are effective drugs.

[0017] The present invention also provides a method for preparing Taohong Huayu Decoction, comprising the following steps:

[0018] S1. Weigh the raw materials in proportion, dry them, crush them through a 100-200 mesh sieve, add distilled water, adjust the pH to between 6.2 and 7.0, sterilize, and cool to obtain a fermentation matrix; inoculate the strain into the fermentation matrix, perform aerobic fermentation, and obtain a fermentation liquid; in parts by mass, the weight parts of each raw material are: 5-10 parts of safflower, 8-15 parts of angelica, 5-8 parts of peach kernel, 5-8 parts of chuanxiong, 8-15 parts of tuckahoe, 8-15 parts of ginseng, 8-15 parts of salvia miltiorrhiza, 5-8 parts of licorice, and 8-15 parts of Achyranthes bidentata;

[0019] S2, centrifuging the fermentation liquid, pouring out the supernatant, and obtaining a fermentation supernatant and a filter residue; adding water and ultrasonically extracting the filter residue at 40-60kHz for 30-60min, centrifuging to obtain a fermentation filter residue liquid, and combining the supernatant and the fermentation filter residue liquid to obtain a fermentation liquid;

[0020] S3, adding modified collagen fibers to the fermentation broth to obtain a purified solution; filtering the purified solution through a 20nm-50nm ultrafiltration membrane to obtain a secondary purified solution;

[0021] S4. Add the flavor corrector to the secondary purified liquid, stir evenly, let stand, filter, fill and sterilize.

[0022] The mass ratio of the raw material powder after passing through the 100-200 sieve in step S1 to distilled water is 1:10-20.

[0023] The inoculation amount of the bacterial strain in step S1 is 1wt%-5wt%.

[0024] In step S1, the bacterial species is any one of Bacillus coagulans, Bacillus subtilis and Bacillus licheniformis. Preferably, the bacterial species is Bacillus coagulans.

[0025] In step S2, the fermentation liquid is centrifuged at a speed of 2000-4000 rpm for 10-20 min.

[0026] In step S4, the flavoring agent is at least one of stevia, sorbitol, and xylitol.

[0027] The modified collagen fiber is prepared by the following method:

[0028] Step 1, remove hair, degrease and cut 20-30 parts of cowhide, add 100-200 parts of 30-50wt% NaOH aqueous solution, heat to 30-40°C, stir and react for 10-14h, remove the solid, rinse with water 3-5 times, and dry at 40-60°C for 6-8h; add the dried cowhide to 200-300 parts of water, slowly add 10-20 parts of 80-98% concentrated sulfuric acid under stirring, then heat to 30-40°C, stir and react for 10-14h, then filter, freeze-dry the solid at minus 50 to minus 60°C for 48-72h to obtain collagen fiber;

[0029] Step 2, weigh 1-5 parts of glyoxylic acid, dilute with 10-50 parts of water, adjust the pH to 6.0-6.5 with a 5-10% NaHCO3 aqueous solution, and then add 10-20 parts of methanol to obtain a mixed solution, add 5-15 parts of collagen fibers to the above solution, stir and react for 1-3 hours, adjust the pH to 6.0-6.5 with a 5-10% NaHCO3 aqueous solution, add 1-5 parts of boric acid and continue to react for 1-3 hours; then, slowly add 10-50 parts of 0.5-1.5wt% glutaraldehyde aqueous solution, stir and react at 30-50°C for 4-6 hours, filter, wash the solid with water 3-5 times, and then dry at 40-50°C for 10-16 hours to obtain modified collagen fibers; the parts refer to parts by weight.

[0030] As a further illustration of the present invention, the deterioration of Chinese medicine decoction is because the decoction contains macromolecular nutrients such as proteins and polysaccharides, which are easy to provide nutritional conditions for the growth of microorganisms. Collagen fibers contain abundant active groups such as -COOH, -OH, -NH2, -CONH2 and -CONH-. Modified collagen fibers introduce some carboxyl groups and boric acid groups, which can adsorb macromolecular nutrients such as proteins and polysaccharides to improve their hydrophilicity. The carboxylic acid group dissociates into negatively charged carboxyl radicals in the solution, which can be adsorbed by electrostatic attraction with the positively charged areas on the surface of the protein; the boric acid functional group can specifically bind to the glycosyl part in the glycoprotein, because the boric acid and the cis-diol structure in the carbohydrate molecule can form a stable five-membered ring complex.

[0031] Beneficial effects of the present invention: The Taohong Huayu Decoction of the present invention can be used to prevent and treat deep vein thrombosis. Compared with the currently commonly used drugs for treating deep vein thrombosis in clinical practice, the composition of the present invention is safer, more reliable and more efficient to use. The Taohong Huayu Decoction of the present invention can also warm the meridians and dispel cold, dispel wind and remove dampness, thereby achieving the purpose of short-term improvement and long-term eradication of deep vein thrombosis. It also has the advantages of being easy to carry and having a long shelf life. DETAILED DESCRIPTION

[0032] The sources of some substances in the examples are as follows:

[0033] Bacillus coagulans, CICC 24625, a commercial product, was purchased from China Industrial Microbiological Culture Collection Center;

[0034] Bacillus licheniformis, CICC 10332, a commercial product, was purchased from China Industrial Microbiological Culture Collection Center;

[0035] Bacillus subtilis, CICC 10732, a commercial product, was purchased from China Industrial Microbiological Culture Collection Center;

[0036] Glyoxylic acid CAS: 298-12-4;

[0037] Glutaraldehyde CAS: 111-30-8.

[0038] Example 1

[0039] A method for preparing Taohong Huayu Decoction comprises the following steps:

[0040] Weigh 6 g of safflower, 10 g of angelica, 6 g of peach kernel, 6 g of Chuanxiong, 10 g of Poria, 10 g of ginseng, 10 g of Salvia miltiorrhiza, 6 g of liquorice, and 10 g of Achyranthes bidentata, dry and grind them through a 160-mesh sieve, add 800 g of distilled water, heat and reflux at 102°C and extract twice, each extraction time is 1 h, combine the filtrates, add 0.1 g of stevioside, stir evenly, filter, fill, and sterilize to obtain Taohong Huayu Decoction.

[0041] Example 2

[0042] A method for preparing Taohong Huayu Decoction comprises the following steps:

[0043] S1. Weigh 6 g of safflower, 10 g of angelica, 6 g of peach kernel, 6 g of chuanxiong, 10 g of tuckahoe, 10 g of ginseng, 10 g of salvia miltiorrhiza, 6 g of liquorice, and 10 g of cyathula, dry them, grind them through a 160-mesh sieve, add 800 g of distilled water, adjust the pH to 6.5, sterilize them with high-pressure steam at 121° C. for 30 min, and cool them to room temperature to obtain a fermentation medium; inoculate the fermentation medium with a strain of 3 wt % and place it in a constant temperature shaker at 37° C. for aerobic fermentation for 3 days to obtain a fermentation liquid;

[0044] S2, centrifuging the fermentation broth at a speed of 4000 rpm for 15 min, pouring out the supernatant to obtain a fermentation supernatant and a residue; adding 200 g of distilled water to ultrasonically extract the residue at 50 kHz for 45 min, centrifuging to obtain a fermentation residue liquid, and combining the supernatant and the fermentation residue liquid to obtain a fermentation broth;

[0045] S3, adding 0.2 g of modified collagen fiber to the fermentation broth, standing for 30 min, centrifuging at 4000 rpm for 15 min, taking the supernatant to obtain a purified solution; filtering the purified solution through a 50 nm ultrafiltration membrane to obtain a secondary purified solution;

[0046] S4. Add 0.1 g of stevioside to the secondary purified liquid, stir evenly, let stand, filter, fill and sterilize.

[0047] The bacterial species is Bacillus coagulans.

[0048] The modified collagen fibers were prepared by the following method:

[0049] Step 1, remove hair, degrease and cut 25g of cowhide, add it into 150g of 40wt% NaOH aqueous solution, heat it to 35℃ and stir it for 12h, remove the solid and rinse it with water 4 times, and dry it at 50℃ for 7h; add the dried cowhide into 250g of water, slowly add 15g of 90% concentrated sulfuric acid under stirring, then heat it to 35℃ and stir it for 12h, then filter it, and freeze-dry the solid at minus 55℃ for 56h to obtain collagen fiber;

[0050] Step 2, weigh 3g of glyoxylic acid, dilute it with 30g of water, adjust the pH to 6.5 with a 7% NaHCO3 aqueous solution, and then add 15g of methanol to obtain a mixed solution, add 10g of collagen fibers to the above solution, stir and react for 2h, adjust the pH to 6.5 with a 7% NaHCO3 aqueous solution, add 3g of boric acid and continue to react for 2h; then, slowly add 30g of 1wt% glutaraldehyde aqueous solution, stir and react at 40°C for 5h, filter, wash the solid with water 4 times, and then dry it at 45°C for 12h to obtain modified collagen fibers.

[0051] Example 3

[0052] A method for preparing Taohong Huayu Decoction comprises the following steps:

[0053] S1. Weigh 6 g of safflower, 10 g of angelica, 6 g of peach kernel, 6 g of chuanxiong, 10 g of tuckahoe, 10 g of ginseng, 10 g of salvia miltiorrhiza, 6 g of liquorice, and 10 g of cyathula, dry them, grind them through a 160-mesh sieve, add 800 g of distilled water, adjust the pH to 6.5, sterilize them with high-pressure steam at 121° C. for 30 min, and cool them to room temperature to obtain a fermentation medium; inoculate the fermentation medium with a strain of 3 wt % and place it in a constant temperature shaker at 37° C. for aerobic fermentation for 3 days to obtain a fermentation liquid;

[0054] S2, centrifuging the fermentation broth at a speed of 4000 rpm for 15 min, pouring out the supernatant to obtain a fermentation supernatant and a residue; adding 200 g of distilled water to ultrasonically extract the residue at 50 kHz for 45 min, centrifuging to obtain a fermentation residue liquid, and combining the supernatant and the fermentation residue liquid to obtain a fermentation broth;

[0055] S3, adding 0.2 g of modified collagen fiber to the fermentation broth, standing for 30 min, centrifuging at 4000 rpm for 15 min, taking the supernatant to obtain a purified solution; filtering the purified solution through a 50 nm ultrafiltration membrane to obtain a secondary purified solution;

[0056] S4. Add 0.1 g of stevioside to the secondary purified liquid, stir evenly, let stand, filter, fill and sterilize.

[0057] The bacterial species is Bacillus subtilis.

[0058] The modified collagen fibers were prepared by the following method:

[0059] Step 1, remove hair, degrease and cut 25g of cowhide, add it into 150g of 40wt% NaOH aqueous solution, heat it to 35℃ and stir it for 12h, remove the solid and rinse it with water 4 times, and dry it at 50℃ for 7h; add the dried cowhide into 250g of water, slowly add 15g of 90% concentrated sulfuric acid under stirring, then heat it to 35℃ and stir it for 12h, then filter it, and freeze-dry the solid at minus 55℃ for 56h to obtain collagen fiber;

[0060] Step 2, weigh 3g of glyoxylic acid, dilute it with 30g of water, adjust the pH to 6.5 with a 7% NaHCO3 aqueous solution, and then add 15g of methanol to obtain a mixed solution, add 10g of collagen fibers to the above solution, stir and react for 2h, adjust the pH to 6.5 with a 7% NaHCO3 aqueous solution, add 3g of boric acid and continue to react for 2h; then, slowly add 30g of 1wt% glutaraldehyde aqueous solution, stir and react at 40°C for 5h, filter, wash the solid with water 4 times, and then dry it at 45°C for 12h to obtain modified collagen fibers.

[0061] Example 4

[0062] A method for preparing Taohong Huayu Decoction comprises the following steps:

[0063] S1. Weigh 6 g of safflower, 10 g of angelica, 6 g of peach kernel, 6 g of chuanxiong, 10 g of tuckahoe, 10 g of ginseng, 10 g of salvia miltiorrhiza, 6 g of liquorice, and 10 g of cyathula, dry them, grind them through a 160-mesh sieve, add 800 g of distilled water, adjust the pH to 6.5, sterilize them with high-pressure steam at 121° C. for 30 min, and cool them to room temperature to obtain a fermentation medium; inoculate the fermentation medium with a strain of 3 wt % and place it in a constant temperature shaker at 37° C. for aerobic fermentation for 3 days to obtain a fermentation liquid;

[0064] S2, centrifuging the fermentation broth at a speed of 4000 rpm for 15 min, pouring out the supernatant to obtain a fermentation supernatant and a residue; adding 200 g of distilled water to ultrasonically extract the residue at 50 kHz for 45 min, centrifuging to obtain a fermentation residue liquid, and combining the supernatant and the fermentation residue liquid to obtain a fermentation broth;

[0065] S3, adding 0.2 g of modified collagen fiber to the fermentation broth, standing for 30 min, centrifuging at 4000 rpm for 15 min, taking the supernatant to obtain a purified solution; filtering the purified solution through a 50 nm ultrafiltration membrane to obtain a secondary purified solution;

[0066] S4. Add 0.1 g of stevioside to the secondary purified liquid, stir evenly, let stand, filter, fill and sterilize.

[0067] The bacterial species is Bacillus licheniformis.

[0068] The modified collagen fibers were prepared by the following method:

[0069] Step 1, remove hair, degrease and cut 25g of cowhide, add it into 150g of 40wt% NaOH aqueous solution, heat it to 35℃ and stir it for 12h, remove the solid and rinse it with water 4 times, and dry it at 50℃ for 7h; add the dried cowhide into 250g of water, slowly add 15g of 90% concentrated sulfuric acid under stirring, then heat it to 35℃ and stir it for 12h, then filter it, and freeze-dry the solid at minus 55℃ for 56h to obtain collagen fiber;

[0070] Step 2, weigh 3g of glyoxylic acid, dilute it with 30g of water, adjust the pH to 6.5 with a 7% NaHCO3 aqueous solution, and then add 15g of methanol to obtain a mixed solution, add 10g of collagen fibers to the above solution, stir and react for 2h, adjust the pH to 6.5 with a 7% NaHCO3 aqueous solution, add 3g of boric acid and continue to react for 2h; then, slowly add 30g of 1wt% glutaraldehyde aqueous solution, stir and react at 40°C for 5h, filter, wash the solid with water 4 times, and then dry it at 45°C for 12h to obtain modified collagen fibers.

[0071] Example 5

[0072] A method for preparing Taohong Huayu Decoction comprises the following steps:

[0073] S1. Weigh 6 g of safflower, 10 g of angelica, 6 g of peach kernel, 6 g of chuanxiong, 10 g of tuckahoe, 10 g of ginseng, 10 g of salvia miltiorrhiza, 6 g of liquorice, and 10 g of cyathula, dry them, grind them through a 160-mesh sieve, add 800 g of distilled water, adjust the pH to 6.5, sterilize them with high-pressure steam at 121° C. for 30 min, and cool them to room temperature to obtain a fermentation medium; inoculate the fermentation medium with a strain of 1 wt % and place it in a constant temperature shaker at 37° C. for aerobic fermentation for 3 days to obtain a fermentation liquid;

[0074] S2, centrifuging the fermentation broth at a speed of 4000 rpm for 15 min, pouring out the supernatant to obtain a fermentation supernatant and a residue; adding 200 g of distilled water to ultrasonically extract the residue at 50 kHz for 45 min, centrifuging to obtain a fermentation residue liquid, and combining the supernatant and the fermentation residue liquid to obtain a fermentation broth;

[0075] S3, adding 0.2 g of modified collagen fiber to the fermentation broth, standing for 30 min, centrifuging at 4000 rpm for 15 min, taking the supernatant to obtain a purified solution; filtering the purified solution through a 50 nm ultrafiltration membrane to obtain a secondary purified solution;

[0076] S4. Add 0.1 g of stevioside to the secondary purified liquid, stir evenly, let stand, filter, fill and sterilize.

[0077] The bacterial species is Bacillus coagulans.

[0078] The modified collagen fibers were prepared by the following method:

[0079] Step 1, remove hair, degrease and cut 25g of cowhide, add it into 150g of 40wt% NaOH aqueous solution, heat it to 35℃ and stir it for 12h, remove the solid and rinse it with water 4 times, and dry it at 50℃ for 7h; add the dried cowhide into 250g of water, slowly add 15g of 90% concentrated sulfuric acid under stirring, then heat it to 35℃ and stir it for 12h, then filter it, and freeze-dry the solid at minus 55℃ for 56h to obtain collagen fiber;

[0080] Step 2, weigh 3g of glyoxylic acid, dilute it with 30g of water, adjust the pH to 6.5 with a 7% NaHCO3 aqueous solution, and then add 15g of methanol to obtain a mixed solution, add 10g of collagen fibers to the above solution, stir and react for 2h, adjust the pH to 6.5 with a 7% NaHCO3 aqueous solution, add 3g of boric acid and continue to react for 2h; then, slowly add 30g of 1wt% glutaraldehyde aqueous solution, stir and react at 40°C for 5h, filter, wash the solid with water 4 times, and then dry it at 45°C for 12h to obtain modified collagen fibers.

[0081] Example 6

[0082] A method for preparing Taohong Huayu Decoction comprises the following steps:

[0083] S1. Weigh 6 g of safflower, 10 g of angelica, 6 g of peach kernel, 6 g of chuanxiong, 10 g of tuckahoe, 10 g of ginseng, 10 g of salvia miltiorrhiza, 6 g of liquorice, and 10 g of cyathula, dry them, grind them through a 160-mesh sieve, add 800 g of distilled water, adjust the pH to 6.5, sterilize them with high-pressure steam at 121° C. for 30 min, and cool them to room temperature to obtain a fermentation medium; inoculate the fermentation medium with the strain at an inoculation amount of 5 wt %, place it in a constant temperature shaker at 37° C. for aerobic fermentation for 3 days, and obtain a fermentation liquid;

[0084] S2, centrifuging the fermentation broth at a speed of 4000 rpm for 15 min, pouring out the supernatant to obtain a fermentation supernatant and a residue; adding 200 g of distilled water to ultrasonically extract the residue at 50 kHz for 45 min, centrifuging to obtain a fermentation residue liquid, and combining the supernatant and the fermentation residue liquid to obtain a fermentation broth;

[0085] S3, adding 0.2 g of modified collagen fiber to the fermentation broth, standing for 30 min, centrifuging at 4000 rpm for 15 min, taking the supernatant to obtain a purified solution; filtering the purified solution through a 50 nm ultrafiltration membrane to obtain a secondary purified solution;

[0086] S4. Add 0.1 g of stevioside to the secondary purified liquid, stir evenly, let stand, filter, fill and sterilize.

[0087] The bacterial species is Bacillus coagulans.

[0088] The modified collagen fibers were prepared by the following method:

[0089] Step 1, remove hair, degrease and cut 25g of cowhide, add it into 150g of 40wt% NaOH aqueous solution, heat it to 35℃ and stir it for 12h, remove the solid and rinse it with water 4 times, and dry it at 50℃ for 7h; add the dried cowhide into 250g of water, slowly add 15g of 90% concentrated sulfuric acid under stirring, then heat it to 35℃ and stir it for 12h, then filter it, and freeze-dry the solid at minus 55℃ for 56h to obtain collagen fiber;

[0090] Step 2, weigh 3g of glyoxylic acid, dilute it with 30g of water, adjust the pH to 6.5 with a 7% NaHCO3 aqueous solution, and then add 15g of methanol to obtain a mixed solution, add 10g of collagen fibers to the above solution, stir and react for 2h, adjust the pH to 6.5 with a 7% NaHCO3 aqueous solution, add 3g of boric acid and continue to react for 2h; then, slowly add 30g of 1wt% glutaraldehyde aqueous solution, stir and react at 40°C for 5h, filter, wash the solid with water 4 times, and then dry it at 45°C for 12h to obtain modified collagen fibers.

[0091] Example 7

[0092] A method for preparing Taohong Huayu Decoction comprises the following steps:

[0093] S1. Weigh 6 g of safflower, 10 g of angelica, 6 g of peach kernel, 6 g of chuanxiong, 10 g of tuckahoe, 10 g of ginseng, 10 g of salvia miltiorrhiza, 6 g of liquorice, and 10 g of cyathula, dry them, grind them through a 160-mesh sieve, add 800 g of distilled water, adjust the pH to 6.5, sterilize them with high-pressure steam at 121° C. for 30 min, and cool them to room temperature to obtain a fermentation medium; inoculate the fermentation medium with a strain of 3 wt % and place it in a constant temperature shaker at 37° C. for aerobic fermentation for 3 days to obtain a fermentation liquid;

[0094] S2, centrifuging the fermentation broth at a speed of 4000 rpm for 15 min, pouring out the supernatant to obtain a fermentation supernatant and a residue; adding 200 g of distilled water to ultrasonically extract the residue at 50 kHz for 45 min, centrifuging to obtain a fermentation residue liquid, and combining the supernatant and the fermentation residue liquid to obtain a fermentation broth;

[0095] S3, adding 0.2 g collagen fiber to the fermentation liquid, letting it stand for 30 min, centrifuging at 4000 rpm for 15 min, taking the supernatant to obtain a purified liquid; filtering the purified liquid through a 50 nm ultrafiltration membrane to obtain a secondary purified liquid;

[0096] S4. Add 0.1 g of stevioside to the secondary purified liquid, stir evenly, let stand, filter, fill and sterilize.

[0097] The bacterial species is Bacillus coagulans.

[0098] Collagen fibers were prepared by the following method: 25 g of cowhide was dehaired, defatted, and chopped, added to 150 g of a 40 wt% NaOH aqueous solution, heated to 35°C and stirred for reaction for 12 h, the solid was removed and rinsed with water 4 times, and dried at 50°C for 7 h; the dried cowhide was added to 250 g of water, 15 g of 90% concentrated sulfuric acid was slowly added under stirring, then heated to 35°C and stirred for reaction for 12 h, then filtered, and the solid was freeze-dried at -55°C for 56 h to obtain collagen fibers.

[0099] Comparative Example 1

[0100] A method for preparing Taohong Xiaoyu Decoction comprises the following steps:

[0101] Weigh 18 g of Salvia miltiorrhiza, 18 g of Houttuynia cordata, 12 g of Achyranthes bidentata, 12 g of Radix Angelicae Sinensis, 12 g of Olibanum, 6 g of Peach Kernel, and 6 g of Carthamus tinctorius, dry and grind them through a 160-mesh sieve, add 800 g of distilled water, heat and reflux at 102°C and extract twice, each extraction time is 1 h, combine the filtrates, add 0.1 g of stevioside, stir evenly, filter, fill, and sterilize to obtain Taohong Xiaoyu Decoction.

[0102] Test Example 1

[0103] The experimental mice were randomly divided into groups, half male and half female, and ten mice in each group corresponded to Examples 1-7, Comparative Example 1 and a blank control group. Carrageenan was used as an inflammatory agent for administration, and 0.1 mL / 10 g was administered to each mouse for three days. 1% carrageenan was injected into the right hind leg of the mouse in an amount of 0.03 ml / mouse. 4-24 hours after the injection, dark red thrombi appeared in the tail of the mouse, gradually expanding to a certain range at the root of the tail. After 30-48 hours, the purple turned into black and the boundary with the normal tail was obvious. The mice were fed with samples of Examples 1-7 and Comparative Example 1, and the drugs were administered twice a day. The blank group was not administered. After 7 days, the percentage of thrombus length was measured. The results are shown in Table 1.

[0104] Table 1

[0105] No. / Project Thrombosis incidence % Thrombus length percentage Example 1 100 39.68±5.74 Example 2 100 1.63±0.91 Example 3 100 17.43±3.05 Example 4 100 16.33±3.01 Example 5 100 9.87±1.32 Example 6 100 9.28±1.30 Example 7 100 2.35±1.06 Comparative Example 1 100 58.16±8.31 blank 100 92.14±5.31

[0106] By comparing Examples 1-7, Comparative Example 1 and the blank group, it can be found that Example 2 has the lowest percentage of thrombus length and the best therapeutic effect on thrombus. The possible reason is that the enzyme produced by the Bacillus coagulans fermentation system has the best separation effect on medicinal plant tissues and can dissolve more effective components. The enzyme produced by the Bacillus coagulans fermentation system further decomposes and transforms the dissolved components into new active ingredients, produces new medicinal effects, and promotes the therapeutic effect.

[0107] By comparing Example 2 with Examples 5-6, it can be found that Example 2 has the best effect. The possible reason is that the number of bacterial strains at a 3% inoculation amount is more appropriate, and the competition between bacterial strains is fierce at a high concentration, which may cause the death of some strains or reduce metabolic activity, affecting the dissolution and conversion of effective ingredients; at a low concentration, the amount of enzyme produced during the growth period is low, and the dissolution and conversion rate of the active ingredients is slower.

[0108] By comparing Example 1 with Comparative Example 1, it can be found that the percentage of thrombus length in Example 1 is lower than that in Comparative Example 1. The possible reason is that although peach kernel, safflower and salvia miltiorrhiza are also added as blood-activating and stasis-removing medicinal materials, Example 1 adds ginseng, angelica and other medicinal materials for nourishing yin and liver, nourishing blood and regulating menstruation, as well as Chuanxiong, Poria and other medicinal materials for promoting qi and removing dampness, strengthening the spleen and calming the mind, so the comprehensive conditioning and treatment effects are better.

[0109] Test Example 2

[0110] The Taohong Huayu Decoction prepared in Example 2, Example 7 and Comparative Example 1 were stored under the same conditions, and samples were taken every 7 days to determine the shelf life based on whether the number of microorganisms exceeded the standard. The number of colonies was tested according to the "Standards for Microbial Limits of Non-sterile Drugs" in General Rules 1107 of Part IV of the 2020 edition of the Pharmacopoeia of the People's Republic of China. According to the standard experiment, the total number of aerobic bacteria (cfu / g or cfu / ml) was ≤10 5 ; Total number of molds and yeasts (cfu / g or cfu / ml) ≤ 10 3 ; Escherichia coli shall not be detected (1g or 1ml); Salmonella shall not be detected (10g or 10ml); bile-resistant Gram-negative bacteria should be less than 10 4 cfu (1g or 1ml). The shelf life of Taohong Huayu Decoction was recorded. The results are shown in Table 2.

[0111] Table 2

[0112] Project / No. Example 2 Example 7 Comparative Example 1 Shelf life (days) 357 91 21

[0113] Through Example 2, Example 7 and Comparative Example 1, it can be found that Example 2 has a longer shelf life. The possible reason is that modified collagen fibers are added. Collagen fibers contain abundant active groups such as -COOH, -OH, -NH2, -CONH2 and -CONH-, which can adsorb substances. Modified collagen fibers introduce some carboxyl groups and boric acid groups. The carboxylic acid groups dissociate into negatively charged carboxyl radicals in the solution, which can be adsorbed with the positively charged areas on the surface of the protein through electrostatic attraction; the boric acid functional group can specifically bind to the glycosyl part in the glycoprotein, which is due to the fact that the cis-diol structure of boric acid and the carbohydrate molecule can form a stable five-membered ring complex. At the same time, the microbial fermentation process can decompose the macromolecular substances in the raw materials into small molecular substances and produce some new metabolites. Bacillus coagulans can produce a variety of antibacterial substances such as coagulants and organic acids during the fermentation process, which can penetrate the cell membrane of harmful bacteria, affect the metabolic pathways of microorganisms, interfere with the synthesis of proteins and metabolic intermediates, and affect the normal growth and reproduction of cells, thereby improving the shelf life.

Claims

1. A method for preparing Taohong Huayu Decoction, characterized in that: In parts by weight, weigh 5-10 parts of safflower, 8-15 parts of angelica, 5-8 parts of peach kernel, 5-8 parts of chuanxiong, 8-15 parts of poria, 8-15 parts of ginseng, 8-15 parts of salvia miltiorrhiza, 5-8 parts of liquorice, 8-15 parts of Achyranthes bidentata, dry and grind them through a 100-200 mesh sieve, add 600-1100 parts of water, heat and reflux at 100-105° C. for extraction 1-3 times, each extraction time is 1-2 h, combine the filtrate, add 0.1-0.5 parts of stevioside, stir evenly, filter, fill and sterilize to obtain Taohong Huayu Decoction.

2. A method for preparing Taohong Huayu Decoction, characterized in that: The steps include: S1. Weigh safflower, angelica, peach kernel, chuanxiong, tuckahoe, ginseng, salvia miltiorrhiza, liquorice and Achyranthes bidentata in proportion, dry and grind through a 100-200 mesh sieve, add water, adjust the pH to between 6.2 and 7.0, sterilize and cool to obtain a fermentation medium; inoculate the strain into the fermentation medium, perform aerobic fermentation and obtain a fermentation liquid; S2, centrifuging the fermentation liquid, pouring out the supernatant, and obtaining a fermentation supernatant and a filter residue; adding water and ultrasonically extracting the filter residue at 40-60kHz for 30-60min, centrifuging to obtain a fermentation filter residue liquid, and combining the supernatant and the fermentation filter residue liquid to obtain a fermentation liquid; S3, adding modified collagen fibers to the fermentation liquid to obtain a purified liquid; The purified liquid is filtered through a 20nm-50nm ultrafiltration membrane to obtain a secondary purified liquid; S4, adding the flavor corrector to the secondary purified liquid, stirring evenly, standing, filtering, filling, and sterilizing; The weight proportions of the raw materials are as follows: 5-10 parts of safflower, 8-15 parts of angelica, 5-8 parts of peach kernel, 5-8 parts of chuanxiong, 8-15 parts of tuckahoe, 8-15 parts of ginseng, 8-15 parts of salvia miltiorrhiza, 5-8 parts of liquorice and 8-15 parts of Achyranthes bidentata.

3. The method for preparing Taohong Huayu Decoction according to claim 2, characterized in that: The mass ratio of the raw material powder after passing through the 100-200 sieve in step S1 to water is 1:10-20.

4. The method for preparing Taohong Huayu Decoction according to claim 2, characterized in that: The inoculation amount of the bacterial strain in step S1 is 1wt%-5wt%.

5. The method for preparing Taohong Huayu Decoction according to claim 2, characterized in that: The bacterial species in step S1 is any one of Bacillus coagulans, Bacillus subtilis and Bacillus licheniformis.

6. The method for preparing Taohong Huayu Decoction according to claim 5, characterized in that: The bacterial species in step S1 is Bacillus coagulans.

7. The method for preparing Taohong Huayu Decoction according to claim 2, characterized in that: In step S2, the fermentation liquid is centrifuged at a speed of 2000-4000 rpm for 10-20 min.

8. The method for preparing Taohong Huayu Decoction according to claim 2, characterized in that: In step S3, the modified collagen fiber is prepared by the following method: Step 1, remove hair, degrease and cut 20-30 parts of cowhide, add 100-200 parts of 30-50wt% NaOH aqueous solution, heat to 30-40°C, stir and react for 10-14h, remove the solid, rinse with water 3-5 times, and dry at 40-60°C for 6-8h; add the dried cowhide to 200-300 parts of water, slowly add 10-20 parts of 80-98% concentrated sulfuric acid under stirring, then heat to 30-40°C, stir and react for 10-14h, then filter, freeze-dry the solid at minus 50 to minus 60°C for 48-72h to obtain collagen fiber; Step 2, weigh 1-5 parts of glyoxylic acid, dilute with 10-50 parts of water, adjust the pH to 6.0-6.5 with a 5-10% NaHCO3 aqueous solution, and then add 10-20 parts of methanol to obtain a mixed solution, add 5-15 parts of collagen fibers to the above solution, stir and react for 1-3 hours, adjust the pH to 6.0-6.5 with a 5-10% NaHCO3 aqueous solution, add 1-5 parts of boric acid and continue to react for 1-3 hours; then, slowly add 10-50 parts of 0.5-1.5wt% glutaraldehyde aqueous solution, stir and react at 30-50°C for 4-6 hours, filter, wash the solid with water 3-5 times, and then dry at 40-50°C for 10-16 hours to obtain modified collagen fibers; The parts are parts by weight.

9. The method for preparing Taohong Huayu Decoction according to claim 2, characterized in that: In step S4, the flavoring agent is at least one of stevia, sorbitol, and xylitol.

10. A Taohong Huayu Decoction, characterized in that: Prepared by the method according to any one of claims 1 to 9.