In-situ collection and in-situ resource utilization method of faeces of migrant birds in winter and application thereof
By harvesting reeds before migratory birds arrive and building bionic tree trunks to lure migratory birds to defecate, using straw and functional bacteria to ferment, migratory bird droppings into biological fertilizers, the threat of migratory bird droppings to the eutrophication and public health security of water is solved, and the threat of migratory bird droppings to the eutrophication and public health safety of water is achieved Harmless and resource-based utilization reduces transportation costs and improves operational efficiency.
Patent Information
- Application Number
- CN202411386271.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-30
- Publication Date
- 2024-12-10
AI Technical Summary
The existing technology lacks effective solutions for collecting and harmless use of migratory bird droppings, resulting in eutrophication of water bodies and threats to public health safety.
By harvesting reeds around the wetland before migratory birds arrive, building bionic tree trunks to lure migratory birds to barren agricultural land to discharge manure, and using straw and functional bacteria to ferment, the bird droppings are converted into biological fertilizers, achieving harmless and resource-based utilization.
It effectively reduces the nutritional load of migratory bird droppings in the water environment, kills some pathogens and viruses, saves transportation costs, improves operational efficiency, and uses bird droppings and biological fertilizers for soil improvement and weight gain, and realizes resource utilization.
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Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of resource utilization, and specifically relates to a method for in-situ collection and on-site resource utilization of excrement of overwintering migratory birds and an application thereof. Background Art
[0002] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention, and should not necessarily be regarded as an admission or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art.
[0003] Bird excrement is an important source of global nutrient cycles. According to statistics, seabirds worldwide excrete about 99,000 tons of phosphorus each year, of which 21,800 tons are easily bioavailable. Birds often live in clusters in lakes, wetlands and other aquatic environments. Studies have shown that the dry weight of waterfowl excrement accounts for 2.25%-3.2% of their body weight every day, and the average phosphorus content in bird droppings is 3.36%±4.22%. Bird droppings are the most important source of nutrition for waterfowl inhabiting water bodies. As a high-load nutrient carrier, bird feces excretion brings a large amount of external nutrients to the water environment, changing the nutrient cycle of the water ecology and accelerating the eutrophication of water quality.
[0004] There are a total of 9 migratory bird routes in the world, 4 of which pass through my country. For example, the migratory route in western my country: migratory birds in Xinjiang, western Inner Mongolia, Gansu, Qinghai and Ningxia, fly south in autumn and spend the winter in the western Sichuan Basin and the Yunnan-Guizhou Plateau. Avian influenza virus is a highly contagious disease that seriously threatens global public health security. It can infect many species of wild birds, and almost all types of avian influenza viruses are detected in migratory bird feces. Relevant evidence shows that migratory birds are often the real source of the spread of avian influenza pathogens, and the disease is distributed worldwide with the migration of migratory birds. Therefore, the harmlessness and resource utilization of migratory bird feces are of great significance for reducing the nutrient load of water bodies and preventing and controlling avian influenza. However, the inventors have found that there is currently no effective collection, in-situ harmlessness and on-site resource utilization plan for migratory bird feces. Summary of the invention
[0005] In view of the deficiencies in the prior art, the present invention provides a method for in-situ collection and on-site resource utilization of wintering migratory bird droppings and its application. Specifically, the present invention provides a favorable living environment for migratory birds by harvesting reeds around wetlands before the arrival of migratory birds; when migratory birds arrive, audio, water, animal and plant food sources are used to lure migratory birds to the bionic tree trunks on the barren agricultural land near their habitats, and the excreted bird droppings are fermented and decomposed under the synergistic effect of the straw biomass and functional bacteria below; after the migratory birds fly away in the spring of the following year, the treated products are applied on-site to improve barren land and increase crop fertility, thereby achieving the effects of harmlessness and resource utilization. Based on the above research results, the present invention is completed.
[0006] Specifically, the present invention relates to the following technical solutions:
[0007] The first aspect of the present invention provides a method for in-situ collection and on-site resource utilization of wintering migratory bird feces, the method comprising:
[0008] S1. Collect and crush straw to obtain biomass materials, and place them on farmland for later use;
[0009] S2, attract migratory birds and collect bird droppings;
[0010] S3. The guano is mixed and fermented to obtain guano bio-fertilizer, and the first compound bacterial agent, the second compound bacterial agent and the third compound bacterial agent are applied at different time periods during the fermentation process.
[0011] Wherein, the first composite bacterial agent can be a combination of Bacillus subtilis, Streptomyces tenuifolius and Trichoderma longibrachiatum.
[0012] The second composite bacterial agent may be a combination of Bacillus mycoides and Bacillus amyloliquefaciens.
[0013] The third composite bacterial agent may be a combination of Lactobacillus acidophilus and Bacillus methylotrophus.
[0014] The second aspect of the present invention provides the use of the guano biofertilizer obtained by the above method in soil improvement and fertilization.
[0015] Beneficial technical effects of one or more of the above technical solutions:
[0016] The above technical scheme realizes the harmless treatment and resource utilization of migratory bird droppings. Migratory bird droppings have potential adverse effects on eutrophication of water bodies and public health safety due to their high nutritional load and potential infectious viruses. The present invention uses various methods to lure migratory birds to gather on poor agricultural land near their habitats, effectively reducing the nutritional load of migratory bird droppings in the water environment; the bird droppings are decomposed and some pathogens and viruses are killed through the synergistic effect of straw biomass and degradation bacteria, achieving the harmless effect of bird droppings; and then they are applied on-site to improve and fertilize agricultural land, realizing the resource utilization of migratory bird droppings.
[0017] At the same time, effective protection of migratory birds is achieved. Reeds have strong reproductive capacity and are widely distributed in wetland areas. They are common invasive species and pose a threat to ecosystems and bird habitats. The above technical solution creates an open habitat for migratory birds by harvesting and cleaning reed populations before the arrival of migratory birds in winter. The migratory bird feces are collected in situ and lured in a safe way, which does not harm the migratory birds and does not affect their migration and foraging.
[0018] The in-situ treatment and on-site application of migratory bird droppings greatly saves transportation costs and improves operational efficiency. Migratory birds are lured to gather on barren agricultural land near their habitats, and the excrement is fermented and decomposed with the straw biomass and functional bacteria below. After the migratory birds fly away in the spring of the following year, the treated products are applied on-site to improve and fertilize the barren land. The entire process involves in-situ collection, in-situ treatment, and on-site application, which greatly saves transportation costs and improves operational efficiency.
[0019] Harmless treatment and resource utilization of reeds and agricultural straw. The common method of dealing with agricultural straw is burning, but it causes air pollution and nutrient loss; the collected reeds and agricultural straw are crushed and mixed, and then fermented with bird droppings and functional bacteria to produce biological organic fertilizer, which achieves the effect of harmlessness and resource utilization. At the same time, the biological fertilizer produced not only has good fertilizer effect, but also can effectively improve the anti-caking property of the biological fertilizer, and has good practical application value. DETAILED DESCRIPTION
[0020] It should be noted that the following detailed descriptions are illustrative and are intended to provide further explanation of the present application. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which the present application belongs.
[0021] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprising" and / or "including" are used in this specification, it indicates the presence of features, steps, operations, devices, components and / or combinations thereof. If the experimental methods of specific conditions are not indicated in the following specific embodiments, they are generally in accordance with the conventional methods and conditions of molecular biology within the art, and such techniques and conditions are fully explained in the literature.
[0022] The present invention is further described in conjunction with specific examples. The following examples are only for explaining the present invention and are not intended to limit the content thereof. If the specific experimental conditions are not specified in the examples, they are usually carried out under conventional conditions or under conditions recommended by the sales company; the materials and reagents used in the examples, unless otherwise specified, can be purchased through commercial channels.
[0023] In a typical specific embodiment of the present invention, a method for in-situ collection and on-site resource utilization of wintering migratory bird feces is provided, the method comprising:
[0024] S1. Collect and crush straw to obtain biomass materials, and place them on farmland for later use;
[0025] S2, attract migratory birds and collect bird droppings;
[0026] S3. The guano is mixed and fermented to obtain guano bio-fertilizer, and the first compound bacterial agent, the second compound bacterial agent and the third compound bacterial agent are applied at different time periods during the fermentation process.
[0027] Wherein, in step S1, the straw may be corn straw, rice straw, rape straw and / or reed straw; preferably, it is a combination of corn straw and reed straw.
[0028] In another specific embodiment of the present invention, the steps of step S1 include: collecting corn stalks one month after the harvest of crops in autumn, crushing them into particles with a diameter of 0.2-1 cm; harvesting and cleaning the wetland reeds near the migratory bird habitat, leaving the reeds naturally for 1-2 weeks, and then crushing them into particles with a diameter of 0.5-1 cm; placing the corn stalks and reed stalks particles on barren farmland for standby use. Furthermore, the mass ratio of corn stalk particles to reed stalk particles is 1-3:1, preferably 2:1; by crushing corn stalks and reed stalks into particles of different sizes and using them in combination, and cooperating with subsequent microbial fermentation, it is beneficial to the fermentation and decomposition of bird droppings, thereby improving the fertility effect of the final bird droppings biofertilizer.
[0029] In another specific embodiment of the present invention, in step S2, the method of luring migratory birds may include but is not limited to luring with habitats, luring with sounds, luring with food and water sources, etc. For example, a bionic tree trunk is built on (poor) farmland near the migratory bird habitat, a small audio player is set at the bottom of the bionic tree trunk, and pre-recorded bird sounds are played intermittently; gullies are set on the surface of the bionic tree trunk, and mealworms are placed in the gullies; corn cobs are hung on the tree trunk; and a water source bucket is set 10-20 meters from the edge of the bionic tree trunk. The whole process continues until the migratory birds migrate and leave in the spring of the following year.
[0030] The bionic tree trunk may be a dead wood assembled tree trunk, a cement simulated tree, etc., which is not specifically limited here.
[0031] The intermittent playing may be playing the bird sounds for 2-5 minutes every 3-4 hours.
[0032] The mealworm is a kind of excellent feed insect with the characteristics of high protein, high nutrition, easy to raise and reproduce. In another specific embodiment of the present invention, the mealworm is placed in a method of first mixing the mealworm with bran in a mass ratio of 1:1, and then placing it in the gully of the tree trunk so that it does not fall. The placement amount is about 100g of the mixture of mealworm and bran per tree trunk; and it is supplemented every 7-10 days.
[0033] The corn cobs are placed in an amount of 2-4 cobs hung on each tree trunk, and are replenished every 7-10 days.
[0034] In another specific embodiment of the present invention, in step S3, the first composite bacterial agent can be a combination of Bacillus subtilis CICC 10089, Streptomyces microflavus CICC23626 and Trichoderma longibrachiatum CICC 41185. The cell count ratio of Bacillus subtilis, Streptomyces microflavus and Trichoderma longibrachiatum in the first composite bacterial agent is 1-100:0.01:0.1.
[0035] The second composite bacterial agent may be a combination of Bacillus mycoides CICC 21473 and Bacillus amyloliquefaciens CICC 21746. The cell count ratio of Bacillus mycoides to Bacillus amyloliquefaciens in the second composite bacterial agent is 0.1-1:1.
[0036] The third composite bacterial agent may be a combination of Lactobacillus acidophilus CICC 6082 and Bacillus methylotrophicus CICC 23921. The cell number ratio of Lactobacillus acidophilus to Bacillus methylotrophicus in the third composite bacterial agent is 0.1-1:10.
[0037] When the above-mentioned composite bacterial agents are applied, the total number of bacteria in each composite bacterial agent can be controlled to (5-10)*10 7 CFU / mL; the amount of each bacterial agent applied was 0.2%-1% (w / w) of the total material at that time.
[0038] It should be noted that the above-mentioned strains were purchased from the China Industrial Microbiological Culture Collection Center and are strains that meet the technical standards of NY / T1109-2017.
[0039] In another specific embodiment of the present invention, the specific method of step S3 includes: placing the biomass material obtained in step S1 under the bionic tree trunk and spraying the first composite bacterial agent; after collecting the bird droppings for 15-20 days, spraying the second composite bacterial agent, and mixing the biomass and the bird droppings evenly; every 30-40 days, spreading 60-70% of the mass of the biomass material of the first biomass material, and spraying the second composite bacterial agent for mixing; after the migratory birds migrate away, adding the third composite bacterial agent again, and mixing the fermentation products, piling them, and standing them for 5-10 days to obtain the bird droppings bio-fertilizer.
[0040] In another specific embodiment of the present invention, there is provided the use of the guano biofertilizer obtained by the above method in soil improvement and fertilization.
[0041] In another specific embodiment of the present invention, in the application, soil improvement and fertilization can be carried out on site, thereby further reducing production and transportation costs. The guano biofertilizer is used in soil improvement and fertilization at a dosage of 0.4-1 kg / m 2 .
[0042] The technical solution of the present application is particularly suitable for the treatment of feces produced by migratory birds that migrate and overwinter in the western Sichuan Basin and the Yunnan-Guizhou Plateau.
[0043] The present invention is further explained by the following examples, but it does not constitute a limitation of the present invention. It should be understood that these examples are only used to illustrate the present invention and are not used to limit the scope of the present invention. The development of each embodiment of the present invention and the comparative example are all selected from a migratory bird habitat wetland in Bijie City, Guizhou Province.
[0044] Example 1
[0045] A method for in-situ collection and on-site resource utilization of wintering migratory bird feces, comprising:
[0046] (1) Straw collection. One month after the harvest of crops in autumn, corn straw was collected and crushed into particles with a diameter of 1-2 cm. At the same time, wetland reeds near the migratory bird habitat were cleaned and harvested, and the reeds were left naturally for 2 weeks before being crushed into particles with a diameter of 1-2 cm. The corn straw and reed straw particles were mixed in a mass ratio of 2:1 to obtain biomass materials and placed on barren farmland for later use.
[0047] (2) Attracting migratory birds and collecting bird droppings. Build dead wood assembling trunks on the barren farmland near the migratory bird habitat; set up a small audio player at the bottom of the bionic trunk to play pre-recorded bird sounds intermittently for 5 minutes every 4 hours; set up several grooves on the surface of the bionic trunk, and place a mixture of mealworms and bran with a mass ratio of 1:1 in the grooves, with 100g placed on each trunk. Hang 3 corn cobs on the trunk; set up a water source barrel 10 meters from the edge of the bionic trunk. Replenish the mixture of mealworms and bran, corn cobs and water every 10 days, and this process continues until the migratory birds migrate away in the spring of the following year.
[0048] (3) Mixed fermentation of bird droppings. Place the biomass material obtained in step (1) (3 cm thick) under the bionic tree trunk, and spray the composite bacterial agent I (the bacterial number ratio of Bacillus subtilis CICC 10089, Streptomyces tenuifolius CICC 23626 and Trichoderma longibrachiatum CICC 41185 is 1:0.01:0.1); after collecting the bird droppings for 15 days, spray the composite bacterial agent II (the bacterial number ratio of Bacillus mycoides CICC 21473 and Bacillus amyloliquefaciens CICC 21746 is 1:1), and mix the biomass and bird droppings evenly; every 30 days, spread the biomass material with a thickness of 2 cm, and then spray the composite bacterial agent II. Mix the biomass and bird droppings mixture evenly. After the migratory birds have left, add compound bacterial agent III (the bacterial cell count ratio of Lactobacillus acidophilus CICC 6082 and Bacillus methylotrophus CICC 23921 is 1:10), and mix the fermentation products, pile them, and let them stand to obtain bird manure biofertilizer. The total bacterial cell count in each compound bacterial agent is 5*10 7 CFU / mL, and the amount of each bacterial agent applied each time was 0.5% of the total amount of material at that time.
[0049] (4) Utilization of bird droppings. Bird droppings biofertilizer can be used on site to improve soil fertility. The usage is 0.5kg / m 2 .
[0050] Example 2
[0051] A method for in-situ collection and on-site resource utilization of wintering migratory bird feces, comprising:
[0052] (1) Straw collection. One month after the harvest of crops in autumn, corn straw is collected and crushed into particles with a diameter of 1-2 cm. At the same time, wetland reeds near the migratory bird habitat are cleaned and harvested, and the reeds are left naturally for 2 weeks before being crushed into particles with a diameter of 1-2 cm. The corn straw and reed straw particles are mixed in a mass ratio of 1:1 to obtain biomass materials and placed on barren farmland for later use.
[0053] (2) Attracting migratory birds and collecting bird droppings. Build dead wood assembling trunks on the barren farmland near the migratory bird habitat; set up a small audio player at the bottom of the bionic trunk to play pre-recorded bird sounds intermittently for 5 minutes every 4 hours; set up several grooves on the surface of the bionic trunk, and place a mixture of mealworms and bran with a mass ratio of 1:1 in the grooves, with 100g placed on each trunk. Hang 3 corn cobs on the trunk; set up a water source barrel 10 meters from the edge of the bionic trunk. Replenish the mixture of mealworms and bran, corn cobs and water every 10 days, and this process continues until the migratory birds migrate away in the spring of the following year.
[0054] (3) Mixed fermentation of guano. The biomass material obtained in step (1) (3 cm thick) was placed under the bionic tree trunk, and composite bacterial agent I (the bacterial number ratio of Bacillus subtilis CICC 10089, Streptomyces tenuifolius CICC 23626 and Trichoderma longibrachiatum CICC 41185 was 10:0.01:0.1) was sprayed; after 15 days of guano collection, composite bacterial agent II (the bacterial number ratio of Bacillus mycoides CICC 21473 and Bacillus amyloliquefaciens CICC 21746 was 0.5:1) was sprayed, and the biomass and guano were evenly mixed; every 30 days, the biomass material was spread with a thickness of 2 cm, and then the composite bacterial agent II was sprayed, and the biomass and guano mixture was evenly mixed. After the migratory birds have left, add compound bacterial agent III (the bacterial cell count ratio of Lactobacillus acidophilus CICC 6082 and Bacillus methylotrophus CICC 23921 is 0.5:10), and mix the fermentation products, pile them, and let them stand to obtain bird manure biofertilizer. The total bacterial cell count in each compound bacterial agent is 1*10 8 CFU / mL, and the amount of each bacterial agent applied each time was 0.3% of the total substance mass at that time.
[0055] (4) Utilization of bird droppings. Bird droppings biofertilizer can be used on site to improve soil fertility. The usage is 0.5kg / m 2 .
[0056] Example 3
[0057] A method for in-situ collection and on-site resource utilization of wintering migratory bird feces, comprising:
[0058] (1) Straw collection. One month after the harvest of crops in autumn, corn straw was collected and crushed into particles with a diameter of 1-2 cm. At the same time, wetland reeds near the migratory bird habitat were cleaned and harvested, and the reeds were left naturally for 2 weeks before being crushed into particles with a diameter of 1-2 cm. The corn straw and reed straw particles were mixed in a mass ratio of 3:1 to obtain biomass materials and placed on barren farmland for later use.
[0059] (2) Attracting migratory birds and collecting bird droppings. Build dead wood assembling trunks on the barren farmland near the migratory bird habitat; set up a small audio player at the bottom of the bionic trunk to play pre-recorded bird sounds intermittently for 5 minutes every 4 hours; set up several grooves on the surface of the bionic trunk, and place a mixture of mealworms and bran with a mass ratio of 1:1 in the grooves, with 100g placed on each trunk. Hang 3 corn cobs on the trunk; set up a water source barrel 10 meters from the edge of the bionic trunk. Replenish the mixture of mealworms and bran, corn cobs and water every 10 days, and this process continues until the migratory birds migrate away in the spring of the following year.
[0060] (3) Mixed fermentation of guano. The biomass material obtained in step (1) (3 cm thick) was placed under the bionic tree trunk, and composite bacterial agent I (the bacterial number ratio of Bacillus subtilis CICC 10089, Streptomyces tenuifolius CICC 23626 and Trichoderma longibrachiatum CICC 41185 was 5:0.01:0.1) was sprayed; after the guano was collected for 15 days, composite bacterial agent II was sprayed, and the biomass and guano were evenly mixed; every 30 days, the biomass material was spread to a thickness of 2 cm, and then composite bacterial agent II (the bacterial number ratio of Bacillus mycoides CICC 21473 and Bacillus amyloliquefaciens CICC 21746 was 0.5:1) was sprayed, and the biomass and guano mixture were evenly mixed. After the migratory birds have left, add compound bacterial agent III (the bacterial cell count ratio of Lactobacillus acidophilus CICC 6082 and Bacillus methylotrophus CICC 23921 is 1:10), and mix the fermentation products, pile them, and let them stand to obtain bird manure biofertilizer. The total bacterial cell count in each compound bacterial agent is 5*10 7 CFU / mL, and the amount of each bacterial agent applied each time was 0.5% of the total amount of material at that time.
[0061] (4) Utilization of bird droppings. Bird droppings biofertilizer can be used on site to improve soil fertility. The usage is 0.5kg / m 2 .
[0062] Comparative Example 1
[0063] The method is the same as that of Example 1, except that in step (1), only corn stalks are added as biomass material.
[0064] Comparative Example 2
[0065] The method is the same as that of Example 1, except that the spraying of the composite bacterial agent III in step (3) of Example 1 is replaced by the composite bacterial agent II.
[0066] Comparative Example 3
[0067] The method is the same as that of Example 1, except that compound bacterial agent I is used instead of compound bacterial agent II and compound bacterial agent III in step (3) of Example 1.
[0068] Effect verification
[0069] The guano biofertilizers prepared in Example 1 and Comparative Examples 1-3 were tested for relevant indicators, and the results obtained are shown in Table 1. As can be seen from the data results in Table 1, the guano biofertilizer obtained by the method of the present application not only significantly reduces the emission of pathogenic microorganisms and greenhouse gases, but also has a high nutrient content, and the anti-caking rate (shearing method) of the guano biofertilizer is also significantly better than that of the comparative example, effectively achieving the harmlessness and resource utilization of guano.
[0070] Table 1
[0071]
[0072]
[0073] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.
Claims
1. A method for in-situ collection and on-site resource utilization of wintering migratory bird feces, characterized in that: The method comprises: S1. Collect and crush straw to obtain biomass materials, and place them on farmland for later use; S2, attract migratory birds and collect bird droppings; S3. The guano is mixed and fermented to obtain guano bio-fertilizer, and the first compound bacterial agent, the second compound bacterial agent and the third compound bacterial agent are applied at different time periods during the fermentation process.
2. The method according to claim 1, characterized in that In step S1, the straw is corn straw, rice straw, rape straw and / or reed straw; preferably a combination of corn straw and reed straw.
3. The method according to claim 1, characterized in that The steps of step S1 include: collecting corn stalks and crushing them into particles with a diameter of 0.2-1 cm; after the reeds are left naturally, crushing them into particles with a diameter of 0.5-1 cm; further, the mass ratio of corn stalk particles to reed stalk particles is 1-3:
1.
4. The method according to claim 1, characterized in that In step S2, the method of attracting migratory birds includes attracting habitats, attracting sounds, and attracting food and water sources; Further steps include: building a bionic tree trunk on (barren) farmland near a migratory bird habitat, and intermittently playing pre-recorded bird calls; setting up grooves on the surface of the bionic tree trunk, and placing mealworms in the grooves; hanging corn cobs on the trunk; and setting up water barrels 10-20 meters from the edge of the bionic tree trunk.
5. The method according to claim 4, characterized in that The bionic tree trunk is a dead wood assembled tree trunk or a cement simulated tree; The intermittent playback is to play the bird sounds for 2-5 minutes every 3-4 hours; The method for placing the mealworms is to first mix the mealworms and bran in a mass ratio of 1:1, and then place the mixture in the gullies of the tree trunks; the amount of the mixture is about 100g of mealworms and bran per tree trunk; and the mixture is replenished every 7-10 days; The corn cobs are placed in an amount of 2-4 cobs hung on each tree trunk, and are replenished every 7-10 days.
6. The method according to claim 1, characterized in that In step S3, the first composite bacterial agent is a combination of Bacillus subtilis CICC 10089, Streptomyces microflavus CICC23626 and Trichoderma longibrachiatum CICC 41185; the cell number ratio of Bacillus subtilis, Streptomyces microflavus and Trichoderma longibrachiatum in the first composite bacterial agent is 1-100:0.01:0.1; The second composite bacterial agent is a combination of Bacillus mycoides CICC 21473 and Bacillus amyloliquefaciens CICC 21746; the cell number ratio of Bacillus mycoides to Bacillus amyloliquefaciens in the second composite bacterial agent is 0.1-1:
1. The third composite bacterial agent is a combination of Lactobacillus acidophilus CICC 6082 and Bacillus methylotrophicus CICC 23921; the cell number ratio of Lactobacillus acidophilus to Bacillus methylotrophicus in the third composite bacterial agent is 0.1-1:
10.
7. The method according to claim 6, characterized in that When the composite bacterial agent is applied, the total bacterial count in each composite bacterial agent is controlled to be (5-10)*10 7 CFU / mL; the amount of each bacterial agent applied was 0.2%-1% (w / w) of the total material at that time.
8. The method according to claim 6 or 7, characterized in that The specific method of step S3 includes: placing the biomass material obtained in step S1 under the bionic tree trunk, and spraying the first composite bacterial agent; after collecting bird droppings for 15-20 days, spraying the second composite bacterial agent, and evenly mixing the biomass and bird droppings; every 30-40 days, spreading the biomass material accounting for 60-70% of the mass of the first biomass material, and spraying the second composite bacterial agent for mixing; after the migratory birds migrate away, adding the third composite bacterial agent again, and mixing the fermentation products, piling them, and standing them for 5-10 days to obtain the bird droppings bio-fertilizer.
9. Use of the guano biofertilizer obtained by the method according to any one of claims 1 to 8 in soil improvement and fertilization.
10. The use according to claim 9, characterized in that: In the application, soil improvement and fertilization are carried out on site. Furthermore, the guano biofertilizer is used in soil improvement and fertilization at an amount of 0.4-1 kg / m 2 .