Spaceflight purple corn RS3 type resistant starch as well as preparation method and application thereof

Abstract

The invention belongs to the field of spaceflight purple corn RS3 type resistant starch, and particularly relates to spaceflight purple corn RS3 type resistant starch as well as a preparation method and application thereof. The preparation method comprises the following steps: adding water into spaceflight purple corn for blending to obtain starch milk; performing ultrasonic treatment on the starch milk, adjusting the starch milk to be acidic, and gelatinizing to obtain gelatinized starch; pullulanase is added into the gelatinized starch for enzymolysis, high-temperature enzyme deactivation is performed after enzymolysis is completed, then cooling, aging recovery, suction filtration and water washing are performed in sequence, and filter residues are obtained; and drying, crushing and sieving the filter residues. The invention provides a preparation method of spaceflight purple corn RS3 resistant starch, the problem that the prepared RS3 resistant starch is low in content and unstable is solved, and experiments prove that the spaceflight purple corn RS3 resistant starch can reduce the expression of inflammatory factors IL-6 and TNF-alpha in serum, and can reduce the content of the inflammatory factors IL-6 and TNF-alpha by adjusting the proportion of each flora in intestinal flora. Therefore, the steady state of the environment in the intestinal tract is controlled, and the effect of treating ulcerative colitis is achieved.

Description

Technical Field

[0001] The invention belongs to the field of aerospace purple corn RS3 type resistant starch, and specifically relates to aerospace purple corn RS3 type resistant starch, a preparation method and application thereof. Background Art

[0002] RS3 resistant starch, as one of the five common resistant starches, is a new type of dietary fiber with multiple advantages, including low calories, high satiety, slow digestion, and low glycemic index.

[0003] However, the resistant starch content of aerospace purple corn RS3 obtained by the existing preparation method is low and the stability is poor; in addition, the therapeutic effect of resistant starch on ulcerative colitis has not yet been reported.

[0004] Therefore, based on this, the technical solution of the present invention is proposed. Summary of the invention

[0005] In order to solve the problems existing in the prior art, the present invention provides a method for preparing aerospace purple corn RS3 type resistant starch, the preparation method comprising the following steps:

[0006] (1) adding water to aerospace purple corn to obtain starch milk;

[0007] (2) subjecting the starch milk to ultrasonic treatment, adjusting it to acidity and gelatinizing it to obtain gelatinized starch;

[0008] (3) adding pullulanase to the gelatinized starch for enzymolysis, inactivating the enzyme at high temperature, and then cooling, aging, filtering and washing in sequence to obtain a filter residue;

[0009] (4) Drying, crushing and sieving the filter residue to obtain the aerospace purple corn RS3 type resistant starch.

[0010] In order to facilitate understanding of the present invention, the principle of the present invention is described:

[0011] First of all, space purple corn starch is made by carrying purple corn seeds into lunar orbit, and after space flight, genetic mutations occur, and then they are brought back to Earth for breeding. The purple corn used in the experiment is the 7th generation, and its basic characteristics have become stable.

[0012] Secondly, the inventors have long verified that the mechanism of forming RS3 resistant starch is that during the heating process, starch absorbs an appropriate amount of water, causing the starch granules to swell, destroying the crystallization area, and releasing the amylose molecules. During the cooling process, the moderately polymerized starch molecules intertwine with each other to form a double helix structure. These double helical structures are further folded through intermolecular hydrogen bonds, and gradually arranged in an orderly manner to form a tightly packed crystal structure. The formation of the amylose crystallization area prevents amylase from approaching and hinders the amylase active center from binding to the starch molecules, giving the amylose crystallase resistance, and finally forming RS3 resistant starch.

[0013] Preferably, in step (1), the aerospace purple corn is added with water to obtain starch milk with a mass concentration of 5 to 40%.

[0014] Preferably, in step (2), the starch milk is ultrasonically treated at a power of 200-500 W for 10-50 min, then adjusted to a pH of 4-6, and microwave heated at 500-700 W for 60-180 s, and then cooled to 60-65° C. to obtain gelatinized starch.

[0015] Preferably, in step (3), 20 to 200 U / g of pullulanase is added to the gelatinized starch, and enzymolysis is carried out at 40 to 60° C. for 2 to 48 hours. After completion, the enzyme is inactivated at a high temperature of 95 to 100° C. for 15 to 20 minutes, and then cooled to 4 to 6° C. and aged for 24 to 120 hours, and finally filtered and washed with water to obtain a filter residue.

[0016] Preferably, in step (4), the filter residue is dried in a far-infrared hot air combined drying device at a drying temperature of 55° C. and an irradiation height of 180 mm, and then crushed and passed through a 120-mesh sieve to obtain the aerospace purple corn RS3 type resistant starch.

[0017] Based on the same technical concept, another scheme of the present invention is to provide aerospace purple corn RS3 type resistant starch obtained by the above preparation method.

[0018] Based on the same technical concept, another scheme of the present invention is to provide an application of aerospace purple corn RS3 type resistant starch in the preparation of a drug for treating ulcerative colitis.

[0019] Preferably, the drug is a drug that reduces the inflammatory factor IL-6 in serum;

[0020] And / or, the drug is a drug that reduces the inflammatory factor TNF-α in serum;

[0021] And / or, the drug is a drug that increases the proportion of Bacteroidetes;

[0022] And / or, the drug is a drug that reduces the proportion of Firmicutes.

[0023] The beneficial effects of the present invention are:

[0024] The invention provides a method for preparing aerospace purple corn RS3 resistant starch. The obtained resistant starch has a content of 29.13-41.25% and strong resistance to enzymolysis, thereby solving the problem that the prepared RS3 resistant starch has a low content and is unstable. Experiments have also confirmed that the aerospace purple corn RS3 resistant starch can reduce the expression of inflammatory factors IL-6 and TNF-α in serum, and can control the homeostasis of the intestinal environment by regulating the content of short-chain fatty acids in intestinal flora, so as to achieve the effect of treating ulcerative colitis. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0026] Figure 1 It is the data chart of IL-6 factor expression in different groups.

[0027] Figure 2 It is the data chart of TNF-α factor expression in different groups.

[0028] Figure 3 is the composition of intestinal flora at the phylum level in different groups (%).

[0029] Figure 4 It is the diffraction peak spectrum of common aerospace purple corn starch and the aerospace purple corn RS3 type resistant starch of the present invention.

[0030] Figure 5 It is the change graph of RS, RDS and SDS contents.

[0031] Note: Compared with the control group, * represents P < 0.05, ** represents P < 0.01.

[0032] Compared with the model group, # represents P < 0.05, and ## represents P < 0.01. DETAILED DESCRIPTION

[0033] To make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be described in detail below. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other implementation methods obtained by ordinary technicians in this field without creative work belong to the scope of protection of the present invention.

[0034] Example 1

[0035] This embodiment provides a method for preparing aerospace purple corn RS3 resistant starch, the preparation method comprising the following steps:

[0036] (1) adding water to the aerospace purple corn to obtain a starch milk with a mass concentration of 15%;

[0037] (2) subjecting the starch milk to ultrasonic treatment at 300 W power for 30 min, adjusting the pH to 5, and heating with a 600 W microwave for 120 s, and then cooling to 60° C. to obtain gelatinized starch;

[0038] (3) adding 100 U / g of pullulanase to the gelatinized starch, performing enzymolysis at 50° C. for 24 h, and then deactivating the enzyme at 100° C. for 15 min, cooling to 4° C. and aging for 72 h, and finally filtering and washing with water to obtain a residue;

[0039] (4) Drying the filter residue in a far-infrared hot air combined drying device at a drying temperature of 55° C. and an irradiation height of 180 mm, crushing the residue and passing it through a 120-mesh sieve to obtain the aerospace purple corn RS3 resistant starch.

[0040] Example 2

[0041] This embodiment provides a method for preparing aerospace purple corn RS3 resistant starch, the preparation method comprising the following steps:

[0042] (1) adding water to the space purple corn to obtain a starch milk with a mass concentration of 5%;

[0043] (2) subjecting the starch milk to ultrasonic treatment at 200 W power for 50 min, adjusting the pH to 4, and heating with a 500 W microwave for 180 s, and then cooling to 60° C. to obtain gelatinized starch;

[0044] (3) adding 20 U / g of pullulanase to the gelatinized starch, performing enzymolysis at 60° C. for 2 h, and then deactivating the enzyme at 95° C. for 20 min, cooling to 4° C. and aging for 120 h, and finally filtering and washing with water to obtain a filter residue;

[0045] (4) Drying the filter residue in a far-infrared hot air combined drying device at a drying temperature of 55° C. and an irradiation height of 180 mm, crushing the residue and passing it through a 120-mesh sieve to obtain the aerospace purple corn RS3 resistant starch.

[0046] Example 3

[0047] This embodiment provides a method for preparing aerospace purple corn RS3 resistant starch, the preparation method comprising the following steps:

[0048] (1) adding water to the aerospace purple corn to obtain a starch milk with a mass concentration of 40%;

[0049] (2) subjecting the starch milk to ultrasonic treatment at a power of 500 W for 10 min, adjusting the pH to 6, and heating the starch milk with a microwave at 700 W for 60 s, and then cooling the mixture to 65° C. to obtain gelatinized starch;

[0050] (3) adding 200 U / g of pullulanase to the gelatinized starch, performing enzymolysis at 40° C. for 48 h, and then deactivating the enzyme at 100° C. for 15 min, cooling to 6° C. and aging for 24 h, and finally filtering and washing with water to obtain a residue;

[0051] (4) Drying the filter residue in a far-infrared hot air combined drying device at a drying temperature of 55° C. and an irradiation height of 180 mm, crushing the residue and passing it through a 120-mesh sieve to obtain the aerospace purple corn RS3 resistant starch.

[0052] Example 4

[0053] In this example, animal experiments were conducted, and 40 SD rats were selected and divided into a blank group, a model group, a mesalazine group, and an RS3 group (the spaceflight purple corn RS3 resistant starch obtained in Example 1 of the present invention was selected). Modeling was carried out by free drinking water with DSS: the specific operation was to replace drinking water with 2% DSS, and let the rats drink freely for 7 days to construct an ulcerative colitis (UC) model.

[0054] For the control group: normal drinking water and diet were given all the time, and an equal volume of normal saline was gavaged.

[0055] For the model group: drinking water was replaced with 2% DSS, normal diet was administered, and an equal volume of normal saline was administered intragastrically.

[0056] For the mesalazine group (positive control group): drinking water was replaced with 2% DSS, a normal diet was given, and the dose of 0.36 g / kg was given by intragastric administration.

[0057] For RS3 group: drinking water was replaced with 2% DSS, and the mice were fed a normal diet by intragastric administration at a dose of 5 g / kg.

[0058] The results are as follows Figure 1 to Figure 3 As shown, it shows that: Aerospace Purple Corn RS3 resistant starch plays an anti-inflammatory role by reducing the levels of inflammatory factors IL-6 and TNF-α in serum. At the same time, it maintains the homeostasis of the intestinal environment and keeps the intestine healthy by regulating the proportion of intestinal flora, increasing the proportion of Bacteroidetes and reducing the proportion of Firmicutes.

[0059] like Figure 4 As shown, ordinary aerospace purple corn starch produces strong diffraction peaks at 15.1°, 17.1°, 18.0° and 23.1°, showing A-type crystals, while the aerospace purple corn RS3 resistant starch of the present invention (Example 1) shows stronger diffraction peaks at 16.9°, 19.8°, 21.9° and 24.0°, and produces a typical diffraction peak at 5.6°, belonging to a B+V-type structure.

[0060] like Figure 5 As shown, after ultrasonic-enzymatic hydrolysis treatment, the RS of aerospace purple corn RS3 (Example 1) increased from 6.99% to 51.47%, and the corresponding RDS and SDS contents decreased, indicating that after ultrasonic-assisted enzymatic treatment of aerospace purple corn starch, rapidly digestible starch (RDS) and slowly digestible starch (SDS) were reduced, resistant starch (RS) increased, and in vitro digestion resistance was enhanced.

[0061] The above is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art can easily think of changes or substitutions within the technical scope disclosed by the present invention, which should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. A method for preparing aerospace purple corn RS3 resistant starch, It is characterized in that The preparation method comprises the following steps: (1) adding water to aerospace purple corn to obtain starch milk; (2) subjecting the starch milk to ultrasonic treatment, adjusting it to acidity and gelatinizing it to obtain gelatinized starch; (3) adding pullulanase to the gelatinized starch for enzymolysis, inactivating the enzyme at high temperature, and then cooling, aging, filtering and washing in sequence to obtain a filter residue; (4) Drying, crushing and sieving the filter residue to obtain the aerospace purple corn RS3 type resistant starch.

2. The method for preparing the aerospace purple corn RS3 resistant starch according to claim 1, It is characterized in that In step (1), water is added to the aerospace purple corn to obtain starch milk with a mass concentration of 5 to 40%.

3. The method for preparing the aerospace purple corn RS3 resistant starch according to claim 1, It is characterized in that In step (2), the starch milk is ultrasonically treated at a power of 200-500 W for 10-50 min, then adjusted to a pH of 4-6, and heated by microwaves at 500-700 W for 60-180 s, and then cooled to 60-65° C. to obtain gelatinized starch.

4. The method for preparing the aerospace purple corn RS3 resistant starch according to claim 1, It is characterized in that In step (3), 20 to 200 U / g of pullulanase is added to the gelatinized starch, and enzymolysis is carried out at 40 to 60° C. for 2 to 48 hours. After completion, the enzyme is inactivated at a high temperature of 95 to 100° C. for 15 to 20 minutes, and then cooled to 4 to 6° C. and aged for 24 to 120 hours. Finally, the residue is filtered and washed with water to obtain a filter residue.

5. The method for preparing the aerospace purple corn RS3 resistant starch according to claim 1, It is characterized in that In step (4), the filter residue is dried in a far-infrared hot air combined drying device at a drying temperature of 55° C. and an irradiation height of 180 mm, and then crushed and passed through a 120-mesh sieve to obtain the aerospace purple corn RS3 type resistant starch.

6. Aerospace purple corn RS3 type resistant starch obtained by the preparation method according to any one of claims 1 to 5.

7. Use of the aerospace purple corn RS3 resistant starch according to claim 6 in the preparation of a drug for treating ulcerative colitis.

8. The use according to claim 7, It is characterized in that The drug is a drug for reducing the inflammatory factor IL-6 in serum; And / or, the drug is a drug that reduces the inflammatory factor TNF-α in serum; and / or, the drug is a drug that increases the proportion of Bacteroidetes; And / or, the drug is a drug that reduces the proportion of Firmicutes.

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