Paclobutrazol membrane modified nano suspending agent as well as preparation method and application thereof

Abstract

The invention discloses a paclobutrazol membrane modified nano suspending agent as well as a preparation method and application thereof. The paclobutrazol membrane modified nano suspending agent comprises the following components in percentage by mass: 25% of paclobutrazol, 5-8% of zein powder, 0.05% of chitosan, 0.8% of a stabilizer, 0.1-0.15% of a thickening agent, 0.1% of a preservative, 3-5% of ammonium acetate, 3-5% of ethylene glycol, 5-8% of ethanol, 0.2% of an organic silicon defoaming agent, 8% of a film-forming agent and the balance of water. Researches show that compared with 25% paclobutrazol wettable powder, the paclobutrazol membrane modified nano suspending agent provided by the invention can significantly improve the plant dwarfing regulation effect, and has a significant dwarfing effect on eucalyptus, moso bamboo, schima superba and conic.

Description

Technical Field

[0001] The invention belongs to the technical field of plant growth regulators, and specifically discloses a paclobutrazol film-modified nano suspension and a preparation method and application thereof. Background Art

[0002] With the rapid development of power transmission and distribution network construction, the number of lines is increasing, and high-voltage transmission lines need to cross densely forested areas. When the clearance distance between the overhead line and the tree canopy is less than the critical air breakdown distance, a tree flashover fault will occur. At present, the problem of tree barrier hazards caused by super-high trees within the protection range of the line, which endangers the safe operation of overhead power lines, is becoming increasingly prominent, seriously endangering the safe operation of the power grid system. Economic trees such as eucalyptus and bamboo, and dominant tree species in nature reserves such as Schima superba and Cone grow rapidly, and are the main tree barrier hazards in South my country. Traditional obstacle removal uses manual climbing pruning or felling, which has the following disadvantages: 1. Manual climbing is required, and there are certain safety hazards in high-altitude operations, and there have been many accidents of people falling from high altitudes; 2. The labor cost has increased significantly, resulting in an increasing cost of manual obstacle removal; 3. Some hidden danger points are remote, with complex terrain, poor traffic, and difficult to reach; 4. Due to the growth of trees, the area of ​​tree barrier hazards is gradually expanding, but the inefficient manual obstacle removal efficiency cannot meet the current situation. Therefore, it is urgent to seek safe and effective alternatives. Gibberellic acid is an important hormone that controls plant growth. Its synthesis site is the part with vigorous growth, such as young shoots and tender leaves. By spraying exogenous paclobutrazol dwarfing agent on the leaves, the signal transduction pathway of the plant's own endogenous gibberellin synthesis can be blocked, inhibiting the synthesis of gibberellin, thereby slowing down the elongation of plant cells. However, the existing paclobutrazol dwarfing agent has a slightly weak effect on plants, and its dwarfing effect needs to be improved. Summary of the invention

[0003] The purpose of the present invention is to provide a novel paclobutrazol film-modified nano suspension concentrate to achieve the purpose of significantly improving the dwarfing effect on plants, in view of the shortcoming that the existing paclobutrazol dwarfing agent has a slightly weak dwarfing effect on plants.

[0004] To achieve the above object, the present invention adopts the following technical solutions:

[0005] A paclobutrazol film-modified nano suspension comprises the following components in percentage by mass: 25% paclobutrazol, 5-8% zein powder, 0.05% chitosan, 0.8% stabilizer, 0.1-0.15% thickener, 0.1% preservative, 3-5% ammonium acetate, 3-5% ethylene glycol, 5-8% ethanol, 0.2% organosilicon defoamer, 8% film former, and the balance is water.

[0006] Preferably, the paclobutrazol film-modified nano suspension comprises the following components in percentage by mass: 25% paclobutrazol, 5% zein powder, 0.05% chitosan, 0.8% stabilizer, 0.1% thickener, 0.1% preservative, 3% ammonium acetate, 5% ethylene glycol, 8% ethanol, 0.2% organosilicon defoamer, 8% film-forming agent, and the balance is water.

[0007] Preferably, the stabilizer is magnesium aluminum silicate.

[0008] Preferably, the thickener is xanthan gum.

[0009] Preferably, the preservative is sodium benzoate.

[0010] Preferably, the film former is Dow Filmier-17 film former.

[0011] The preparation method of the paclobutrazol film-modified nano suspension of the present invention comprises the following steps:

[0012] (1) firstly adding zein powder to ethanol, adding acetic acid and a small amount of water, controlling the temperature at 70° C., stirring until clear, evaporating two-thirds of the ethanol, and then stirring at room temperature for 24 hours to obtain a zein powder solution;

[0013] (2) preparing a chitosan solution with a mass fraction of 0.5%, adding acetic acid to adjust the pH to 2, then adding a thickener, and stirring to obtain a chitosan solution;

[0014] (3) Add paclobutrazol original drug and stabilizer to ethylene glycol, stir evenly, add zein powder solution at a uniform speed, adjust pH with ammonia water, stir for 30 minutes, add silicone defoamer and preservative, add dropwise to chitosan solution under high-speed stirring, and finally add film-forming agent and remaining water to obtain paclobutrazol film-modified nano suspension.

[0015] In the above preparation method, the amount of acetic acid and ammonia water used is based on the amount of ammonium acetate in the production formula.

[0016] The present invention also provides the application of the paclobutrazol film-modified nano suspension in plant growth regulation.

[0017] Preferably, the plant growth regulation is plant dwarfing regulation.

[0018] Preferably, the paclobutrazol film-modified nano suspension is used in the form of foliar spraying.

[0019] The present invention has the following beneficial effects:

[0020] Compared with the traditional formulation of paclobutrazol, the paclobutrazol film-modified nano suspension provided by the present invention can significantly improve the dwarfing regulation effect of plants. Compared with the clear water control group, 25% paclobutrazol wettable powder reduced the growth rate of eucalyptus, bamboo, Schima superba, and cone plant height by 58.98%, 38.77%, 42.35%, and 59.57%, respectively, while the paclobutrazol film-modified nano suspension of the present invention reduced the growth rate of eucalyptus, bamboo, Schima superba, and cone plant height by 90.02%, 76.00%, 75.51%, and 93.04%, respectively. It can be seen that compared with 25% paclobutrazol wettable powder, the paclobutrazol film-modified nano suspension of the present invention can further significantly reduce the increase in the height of the above-mentioned plants. DETAILED DESCRIPTION

[0021] The following examples are provided to further illustrate the present invention, rather than to limit the present invention.

[0022] Example 1

[0023] A paclobutrazol film-modified nano suspension comprises the following components in percentage by mass: 25% paclobutrazol, 5% zein powder, 0.05% chitosan, 0.8% magnesium aluminum silicate, 0.1% xanthan gum, 0.1% sodium benzoate, 3% ammonium acetate, 5% ethylene glycol, 8% ethanol, 0.2% organosilicon defoamer, 8% Dow Filmier-17, and the balance is water.

[0024] Preparation method:

[0025] (1) First, zein powder is added to ethanol, acetic acid and a small amount of water are added, the temperature is controlled at 70°C, stirred until clear, two-thirds of the ethanol is evaporated, and then stirred at room temperature for 24 hours to obtain a zein powder solution.

[0026] (2) chitosan was prepared into a solution with a mass fraction of 0.5%, acetic acid was added to adjust the pH to about 2, and then xanthan gum was added and stirred to obtain a chitosan solution.

[0027] (3) Add paclobutrazol original drug and magnesium aluminum silicate to ethylene glycol, stir evenly, add zein powder solution at a uniform speed, adjust the pH with ammonia water, stir for 30 minutes, add silicone defoamer and sodium benzoate, and add dropwise to the chitosan solution under high-speed stirring, and finally add Dow Filmier-17 film-forming agent and remaining water to obtain paclobutrazol film-modified nanosuspension.

[0028] Preliminary tests showed that the optimal dosage of paclobutrazol on Schima superba and Cone is 1000 mg / L (25% paclobutrazol wettable powder diluted 250 times), and the optimal dosage on Eucalyptus and Bamboo is 500 mg / L (25% paclobutrazol wettable powder diluted 500 times) and 3000 mg / L (25% paclobutrazol wettable powder diluted 83.3 times), respectively. In order to investigate the dwarfing regulation effect of paclobutrazol film-modified nanosuspension on the above plants, a potted test was conducted. Seedlings of eucalyptus (average seedling height 103 cm), bamboo (average seedling height 236 cm), Schima superba (average seedling height 146 cm), Cone (average seedling height 117 cm) with basically the same specifications were selected. The potting medium was collected from the subtropical forest soil in South China. Before the test, the soil was air-dried and crushed, and stones, roots and other debris were picked out, and sieved through a 2 cm sieve for later use. A round non-woven bag with a diameter of 18 cm and a height of 20 cm was used for potting, and each pot was filled with 5.5 kg of air-dried soil. After 3 months of seedling acclimatization, the above-mentioned paclobutrazol film-modified nanosuspension was used for foliar spraying of the plants. At the same time, a control treatment of clean water and 25% paclobutrazol wettable powder was set. The final concentration of each regulator treatment was the optimal dosage for each plant. A total of 3 treatments were set up, with 10 repetitions. Spray the medicine once on consecutive rainless days in midsummer, and wet the leaves. The growth of the plants was investigated 60 days after spraying. The results are shown in Table 1.

[0029] Table 1. Dwarfing effect of paclobutrazol on eucalyptus, bamboo, Schima superba, and cone

[0030]

[0031]

[0032] * Change range (%) = (increase in treatment - increase in clear water control) / increase in clear water control * 100

[0033] The results in Table 1 show that under the same concentration conditions, conventional 25% paclobutrazol wettable powder treatment can effectively inhibit the growth of eucalyptus, bamboo, Schima superba and cone plants, while the paclobutrazol film-modified nanosuspension of the present invention can further significantly reduce the growth of the above plants.

[0034] The above are only preferred embodiments of the present invention. It should be noted that the above preferred embodiments should not be regarded as limiting the present invention, and the protection scope of the present invention should be based on the scope defined by the claims. For ordinary technicians in this technical field, several improvements and modifications can be made without departing from the spirit and scope of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A paclobutrazol film-modified nanosuspension, characterized in that: The invention comprises the following components in percentage by mass: 25% of paclobutrazol, 5-8% of zein powder, 0.05% of chitosan, 0.8% of stabilizer, 0.1-0.15% of thickener, 0.1% of preservative, 3-5% of ammonium acetate, 3-5% of ethylene glycol, 5-8% of ethanol, 0.2% of organosilicon defoamer, 8% of film-forming agent, and the balance is water.

2. The paclobutrazol film-modified nanosuspension according to claim 1, characterized in that: The invention comprises the following components in percentage by mass: 25% of paclobutrazol, 5% of zein powder, 0.05% of chitosan, 0.8% of stabilizer, 0.1% of thickener, 0.1% of preservative, 3% of ammonium acetate, 5% of ethylene glycol, 8% of ethanol, 0.2% of organosilicon defoamer, 8% of film-forming agent, and the balance is water.

3. The paclobutrazol film-modified nanosuspension according to claim 1, characterized in that: The stabilizer is magnesium aluminum silicate.

4. The paclobutrazol film-modified nanosuspension according to claim 1, characterized in that: The thickener is xanthan gum.

5. The paclobutrazol film-modified nanosuspension according to claim 1, characterized in that: The preservative is sodium benzoate.

6. The paclobutrazol film-modified nanosuspension according to claim 1, characterized in that: The film former is Dow Filmier-17 film former.

7. A method for preparing the paclobutrazol film-modified nanosuspension according to any one of claims 1 to 6, characterized in that: The following steps are involved: (1) firstly adding zein powder to ethanol, adding acetic acid and a small amount of water, controlling the temperature at 70° C., stirring until clear, evaporating two-thirds of the ethanol, and then stirring at room temperature for 24 hours to obtain a zein powder solution; (2) preparing a chitosan solution with a mass fraction of 0.5%, adding acetic acid to adjust the pH to 2, then adding a thickener, and stirring to obtain a chitosan solution; (3) Add paclobutrazol original drug and stabilizer to ethylene glycol, stir evenly, add zein powder solution at a uniform speed, adjust pH with ammonia water, stir for 30 minutes, add silicone defoamer and preservative, add dropwise to chitosan solution under high-speed stirring, and finally add film-forming agent and remaining water to obtain paclobutrazol film-modified nano suspension.

8. Use of the paclobutrazol film-modified nano suspension according to any one of claims 1 to 6 in regulating plant growth.

9. The use according to claim 8, characterized in that: The plant growth regulation is plant dwarfing regulation.

10. The use according to claim 8, characterized in that: The paclobutrazol film-modified nano suspension is used in the form of foliar spraying.

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