Insecticidal composition and application thereof

Abstract

The invention belongs to the field of pesticides, and relates to an insecticidal composition. Effective components of the insecticidal composition comprise an active component A and an active component B, the active component A is a compound shown in a formula (I), the active component B is any one of Flupyroxystrobin, Indazapyroxamet and Nicofluprole, and the mass ratio of the active component A to the active component B is (1: 50)-(34: 1). The insecticidal composition has the advantages of synergism, resistance delay and the like, and is used for preventing and treating resistant pests.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides and insecticides, and particularly relates to an insecticide composition and application thereof. Background Art

[0002] Flupyroxystrobin is a methoxyacrylate insecticide with high activity against Diptera pests such as fruit flies, leafminers, leafminers, and leeks fungus gnats, as well as Thysanoptera thrips. Its chemical name is: (2E)-3-methoxy-2-(2-{[4-(trifluoromethyl)pyridin-2-yl]oxy}phenyl)prop-2-enoic acid methyl ester, and its chemical structure is:

[0003]

[0004] Indazapyroxamet is a pyridine-containing amide insecticide developed by FMC, used to control Thysanoptera or Hemiptera pests such as western flower thrips, potato leafhoppers, aphids, whiteflies, etc. Its chemical name is: N-(1-methylcyclopropyl)-2-(3-pyridyl)-2H-benzopyrazole-4-carboxamide, and its chemical structure is:

[0005]

[0006] Nicofluprole is a nicotinamide compound with a broad spectrum of insecticidal activity, showing excellent insecticidal activity against lepidopteran pests, hemiptera pests and acarina pests. Chemical name: 2-chloro-N-cyclopropyl-5-(1-(2,6-dichloro-4-(perfluoropropane-2-yl)phenyl)-1H-pyrazol-4-yl)-N-methylnicotinamide, and its chemical structure is:

[0007]

[0008] In the process of agricultural production, the long-term continuous high-dose use of a single variety or a single mode of action of chemical pesticides is likely to cause problems such as pest resistance and resistance evolution. Reasonable compounding or mixing of pesticides has the advantages of reducing the amount of pesticides used, improving the control effect, and delaying the occurrence and development of pest resistance and resistance. It is one of the most effective methods to solve the above problems. By mixing the compound of formula (I) with Flupyroxystrobin, Indazapyroxamet, and Nicofluprole, it is possible to quickly control different pests, expand the control spectrum, improve the control effect of the pesticide, and reduce the generation of pest resistance. No reports on this aspect have been found so far. Summary of the invention

[0009] The invention aims to provide an insecticide composition which has synergistic effect, reduces resistance and has low use cost.

[0010] To achieve the above object, the technical solution adopted by the present invention is: an insecticide composition, comprising active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (I), and the structural formula is as follows:

[0011] The active ingredient B is any one of Flupyroxystrobin, Indazapyroxamet, and Nicofluprole;

[0012] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:50 to 34:1;

[0013] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:24 to 28:1;

[0014] Further, the mass ratio of the compound of formula (I) to Flupyroxystrobin is 1:27 to 27:1, such as 1:27, 1:18, 1:9, 2:3, 3:2, 9:2, 18:1, 27:1 or any value between the above values;

[0015] Furthermore, the mass ratio of the compound of formula (I) to Flupyroxystrobin is 1:18 to 27:1;

[0016] Furthermore, the mass ratio of the compound of formula (I) to Flupyroxystrobin is 1:18, 1:9, 2:3, 3:2, 9:2, 18:1, 27:1;

[0017] Further, the mass ratio of the compound of formula (I) to Indazapyroxamet is 1:50 to 30:1, such as 1:50, 1:32, 1:22, 1:16, 1:11, 1:10, 1:6, 2:11, 2:5, 5:2, 10:3, 11:2, 17:1, 18:1, 24:1, 30:1 or any value between the above values;

[0018] Further, the mass ratio of the compound of formula (I) to Indazapyroxamet is 1:32 to 24:1;

[0019] Further, the mass ratio of the compound of formula (I) to Indazapyroxamet is 1:32, 1:22, 1:16, 1:11, 1:10, 1:6, 2:11, 2:5, 5:2, 10:3, 11:2, 17:1, 18:1, 24:1;

[0020] Furthermore, the mass ratio of the compound of formula (I) to Nicofluprole is 1:24 to 34:1, such as 1:24, 1:14, 1:8, 2:3, 7:1, 14:3, 28:1, 34:1 or any value between the above values;

[0021] Furthermore, the mass ratio of the compound of formula (I) to Nicofluprole is 1:24 to 28:1;

[0022] Furthermore, the mass ratio of the compound of formula (I) to Nicofluprole is 1:24, 1:14, 1:8, 2:3, 7:1, 14:3, 28:1;

[0023] Furthermore, based on the total mass of the insecticide composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the insecticide composition is 0.5% to 90%;

[0024] Furthermore, based on the total mass of the insecticide composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the insecticide composition is 1% to 85%;

[0025] Furthermore, the sum of the contents of the active ingredient A and the active ingredient B in the insecticide composition is 1% to 85%;

[0026] Furthermore, the insecticide composition includes agriculturally acceptable auxiliary ingredients in addition to the active ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists or carriers;

[0027] Furthermore, the insecticide composition further comprises an adjuvant, and the adjuvant is selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a synergist and a carrier;

[0028] The wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, alpha olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, saponin powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0029] The dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acid, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0030] The emulsifier is selected from one or more of calcium dodecylbenzene sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0031] The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0032] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0033] The antifreeze agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0034] The defoamer is selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of fatty alcohols; and / or

[0035] The solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel, solvent oil, vegetable oil, vegetable oil derivatives and water; and / or

[0036] The preservative is selected from one or more of propionic acid, sodium propionic acid, sorbic acid, sodium sorbic acid, potassium sorbic acid, benzoic acid, sodium benzoic acid, sodium p-hydroxybenzoic acid, methyl p-hydroxybenzoate, kasone and 1,2-benzisothiazolin-3-one; and / or

[0037] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0038] The synergist is selected from synergist phosphite, synergist ether; and / or

[0039] The carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives.

[0040] Furthermore, the insecticide composition can be prepared into an agriculturally acceptable formulation, and the formulation is selected from a solid formulation and / or a liquid formulation;

[0041] Furthermore, the solid preparation includes powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0042] Further, the liquid preparation includes a soluble solution, a soluble gel, an oil, a film-spreading oil, an emulsifiable concentrate, a latex, a dispersible liquid, an ointment, an aqueous emulsion, an oil emulsion, a microemulsion, a fat, a suspension, a microcapsule suspension, an oil suspension, a dispersible oil suspension, a suspoemulsion, a microcapsule suspension-suspension, a microcapsule suspension-water emulsion or a microcapsule suspension-suspoemulsion;

[0043] Furthermore, the solid preparation is selected from wettable powders and water dispersible granules; the liquid preparation is selected from emulsifiable concentrates, aqueous emulsions, microemulsions, suspensions, suspoemulsions, and dispersible oil suspensions;

[0044] Furthermore, the solid preparation is selected from wettable powders and water dispersible granules; the liquid preparation is selected from emulsifiable concentrates and suspensions;

[0045] Application of the insecticide composition of the present invention in preventing and controlling agricultural and forestry pests and sanitary pests;

[0046] The insecticide composition of the present invention can be used to control pests on fruit trees, vegetables, ornamental plants, tea, cotton, and cereal crops;

[0047] Furthermore, the agricultural and forestry pests and sanitary pests are Lepidoptera and Thysanoptera pests;

[0048] Further, the Lepidoptera pests include: Adoxophyes spp., Adoxophyes orana, Agrotis spp. (cutworms), Agrotis ipsilon (black cutworm), Alabamaargillacea (cotton leafworm), Amorbia cuneana, Amyelosis transitella (navel orangeworm), Anacamptodes defectaria, Anarsia lineatella (peach twig borer), Anomis sabulifera (jute looper), Anticarsia g emma talis, Archips argyrospila (fruittreeleafroller), Archips rose leafroller, rosana(rose leaf roller), Argyrotaenia spp.(tortricid moths), Argyrotaenia citrana(orange tortrix), Autographa gamma, Bonagota cranaodes, Borbo cinnara(rice leaf folder), Bucculatrix thurberiella(cotton leaf perforator), Caloptilia spp.(leaf miners), Capua reticulana, Carposina niponensis(peach fruit moth), Chilo spp., Chlumetia transversa(mango shoot borer), Choristoneura rosaceana(obliquebanded leaf roller), leafroller)), Chrysodeixis spp.), Cnaphalocerus medinalis (grass leafroller), Colias spp., Conpomorpha cramerella, Cossus cossus (carpenter moth), Crambus spp. (sod webworms), Cydia funebrana (plum fruit moth), Cydia molesta (oriental fruit moth), Cydia nignicana (pea moth), Cydia pomonella (codling moth), Darna diducta, Diaphania spp. (stem borer), borers), Diatraea spp. (stalk borers), Diatraea spp. (sugarcane borer), Diatraea graniosella (southwester corn borer), Earias spp. (bollworms), Earias insulata (Egyptian bollworm), Earias vitella (rough northern bollworm), Ecdytopopha aurantianum, Elasmopalpus lignosellus (lesser cornstalk borer), Epiphysias postruttana (light brown apple moth), Ephestia spp. (flour moths), Ephestia cautella)(almond moth), tobacco pink moth.

[0049] Ephestia elutella (tobbaco moth), Ephestiakuehniella (Mediterranean flour moth), Epimeces spp., Epinotia aporema, Erionota thrax (banana skipper), Eupoecilia ambiguella (grape berry moth), Euxoa auxiliaris (army cutworm), Feltia spp. (cutworms), Gortyna spp. (stemblerers), Grapholita molesta (oriental fruit moth), Hedylepta indicate (bean leaf roller), leafwebber), Helicoverpa sp p. (southworm), Helicover paarmigera, Helicoverpa zea, Heliothis spp., Heliothis virescens, Hellula undalis (cabbage webworm), Indarbela spp. (root borers), Keiferia lycopersicella, Leucinodes orbonalis, Leucoptera malifoliella, Lithocollectis spp., Lobesia botrana (grape fruit moth), Loxagrotis spp.), Loxagrotis albicosta (western bean cutworm), Lymantria dispar (gypsy moth), Lyonetia clerkella (apple leaf miner), Mahasena corbetti (oil palm bagworm), Malacosoma spp. (tent caterpillars), Mamestra brassicae (cabbage armyworm), Maruca testulalis (bean pod borer), Metisa plana, Mythimna unipuncta (true armyworm), Neoleucinodes elegantalis, Nymphula depunctalis, Operophtherabrumata, Ostrinia nubilalis (European corn borer), Oxydia vesulia, Pandemis cerasana (common currant tortrix), Pandemis heparana (brown apple tortrix), Papilio demodocus, Pectinophora gossypiella (pink bollworm), Peridroma spp. (cutworm), Peridroma saucia (variegated cutworm), Perileucoptera coffeella (white coffee leafminer), Phthorimaea operculella (potato tuber moth), Phyllocnisitis citrella, Phyllonorycter spp.), Pieris rapae, Plathypena scabra, Plodia interpunctella, Plutellaxylostella (diamondback moth), Polychrosis viteana (grapeberry moth), Prays endocarpa, Prays oleae (olive moth), Pseudaletia spp. (spot moth), Spodoptera litura Fabricius, Pseudaletia unipunctata (armyworm), Pseudoplusia includes (, Rachiplusia nu, Chilo suppressalis (Walker), Scirpophaga incertulas, Sesamia spp.) (stem borers), Sesamia inferens (pink rice stem borer), Spodoptera frugiperda (JESmmith), Sesamia nonagrioides, Setora nitens, Sitotroga cerealella (Angoumois grain moth), Sparganothis pilleriana, Spodoptera spp., Spodoptera exigua, Spodoptera fugiperda, Spodoptera oridania (southern armyworm), Synanthedon spp.), Thecla basilides, Thermisia gemmatalis, Tineola bisselliella (webbing clothesmoth), Trichoplusia ni (cabbage worm), Pierisrapae Linne (cabbage worm), Tuta absoluta;.

[0050] Further, the Thysanoptera pests include: Thrips palmiKarny, Thrips tabaci, Frankliniella fusca (tobacco thrips), Frankliniella occidentalis, (western flower thrips), Frankliniella shultzei, Frankliniella williamsi (cornthrips), Heliothrips haemorrhaidalis, greenhouse thrips, Riphiphorothrips cruentatus, Scirtothrips spp., Scirtothrips citri (citrus thrips), Scirtothrips citri (citrus thrips), and Scirtothrips occidentalis. dorsalis) (yellow tea thrips), Taeniothrips rhopalantennalis, and Thrips spp.;

[0051] Furthermore, the lepidopteran pests are cabbage worm, diamondback moth, beet armyworm, Spodoptera litura, Spodoptera litura, two-spotted armyworm, striped stem borer, and cotton bollworm;

[0052] Furthermore, the Thysanoptera pests are western flower thrips, tobacco thrips, palm thrips, and watermelon thrips;

[0053] The present invention also provides a method for using the insecticidal composition as described above, specifically applying an effective dose to the pests to be controlled or the medium in which they grow.

[0054] The insecticide composition of the present invention has the following beneficial effects:

[0055] 1) The insecticide composition of the present invention exhibits a synergistic effect within a certain ratio range, and has a significant control effect;

[0056] 2) The insecticide composition of the present invention can delay the insecticide resistance, is environmentally friendly, can reduce the amount of pesticides used, and reduces the cost of insect pest control. DETAILED DESCRIPTION

[0057] The present invention is further described below with reference to the examples. The percentages in the examples are all percentages by weight, but the present invention is not limited thereto.

[0058] The composition of the present invention can be provided in the form of a preparation. It can be prepared into a suspension, emulsifiable concentrate, water dispersible granules, aqueous emulsion, granules, wettable powder, dispersible oil suspension, etc. as needed. The content of the active ingredient in the composition of the present invention depends on the application amount when used alone, and also depends on the mixing ratio and the degree of synergistic effect. The optimal range of the active ingredient content varies according to the type of preparation of the composition.

[0059] Preparation Example

[0060] Embodiment 1:

[0061] 25% formula (Ⅰ) compound · flupyroxystrobin suspension (15+10)

[0062] Formula: 15% compound of formula (I), 10% flupyroxystrobin,

[0063] 4% fatty alcohol polyoxyethylene ether phosphate, 2% sodium lignin sulfonate, 2.5% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 1% magnesium aluminum silicate, 0.2% xanthan gum, 0.5% methyl paraben, 5% ethylene glycol, 0.5% silicone defoamer, and deionized water to make up the balance;

[0064] Preparation method: Add active ingredients to the wetting dispersant and defoamer, use zirconium oxide beads, and use a sand mill to wet grind to D 90 (90% of the particle size) <10 μm, and a pulverized slurry is obtained. A thickener, antifreeze agent, preservative, etc. are added to the pulverized slurry and mixed evenly, and deionized water is added to 100%, and high-speed shearing is performed evenly to prepare a suspension preparation of the composition of the present invention.

[0065] Embodiment 2:

[0066] 28% compound of formula (I)·indazapyroxamet suspension (8+20)

[0067] Formula: 8% compound of formula (I), 20% indazapyroxamet,

[0068] 2% sodium lignin sulfonate, 2.5% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 4% alkylphenol polyoxyethylene ether phosphate, 1% magnesium aluminum silicate, 0.2% xanthan gum, 0.5% methyl paraben, 5% ethylene glycol, 0.5% organosilicon defoamer, and deionized water to make up the balance;

[0069] Preparation method: Same as Example 1.

[0070] Embodiment 3:

[0071] 20% compound of formula (I)·nicofluprole suspension (8+12)

[0072] Formula: 8% compound of formula (I), 12% nicofluprole,

[0073] 4.2% styrylphenol ethoxylate phosphate, 2.5% sodium lignin sulfonate, 2.5% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 1% magnesium aluminum silicate, 0.3% xanthan gum, 0.5% sodium sorbate, 5% ethylene glycol, 0.5% silicone defoamer, and deionized water to make up the balance;

[0074] Preparation method: Same as Example 1.

[0075] Embodiment 4:

[0076] 45% compound of formula (I) · flupyroxystrobin water dispersible granules (25+20)

[0077] Formula: 25% compound of formula (I), 20% flupyroxystrobin,

[0078] 2% sodium lignin sulfonate, 6% sodium polycarboxylate, 5% naphthalene sulfonate formaldehyde condensate, 3.5% sodium lauryl sulfate, 5% white carbon black, 2% bentonite, kaolin to make up the balance;

[0079] Preparation method: According to the formula ratio, the active ingredient is added to the carrier, and a surfactant and other functional additives are added thereto, mixed, and after air flow pulverization, appropriate water is added, and then kneading, granulation, drying, and sieving can be made into a water-dispersible granule preparation of the composition of the present invention.

[0080] Embodiment 5:

[0081] 50% compound of formula (I)·indazapyroxamet water dispersible granules (37.5+22.5)

[0082] Formula: 37.5% compound of formula (I), 22.5% indazapyroxamet,

[0083] 6% sodium polycarboxylate, 2.5% sodium lignin sulfonate, 6% naphthalene sulfonate formaldehyde condensate, 3.5% sodium lauryl sulfate, 3% ammonium sulfate, 4% white carbon black, 2% bentonite, and kaolin make up the balance;

[0084] Preparation method: Same as Example 4.

[0085] Embodiment 6:

[0086] 1.4% compound of formula (I)·indazapyroxamet aqueous emulsion (1+0.4)

[0087] Formula: 1% compound of formula (I), 0.4% indazapyroxamet,

[0088] 12% trimethylbenzene, 10% cyclohexanone, 1% calcium dodecylbenzene sulfonate, 1% sodium sulfate of fatty alcohol polyoxyethylene ether, 6.5% EO / PO block copolymer, 5% propylene glycol, 1.5% glycerol, 0.1% organosilicon defoamer, 0.2% xanthan gum, 0.1% sodium benzoate, and deionized water to make up the balance;

[0089] Preparation method: according to the formula ratio of the embodiment, the active ingredient is dissolved in the solvent and an emulsifier is added to dissolve it into a uniform oil phase, and deionized water and antifreeze are mixed together to form a uniform water phase; under high-speed shearing, the water phase is added to the oil phase to form a well-dispersed water emulsion preparation.

[0090] Embodiment 7:

[0091] 5.2% compound of formula (Ⅰ)·nicofluprole aqueous emulsion (3.2+2)

[0092] Formula: 3.2% compound of formula (I), 2% nicofluprole,

[0093] 15% cyclohexanone, 12% trimethylbenzene, 1% styrylphenol ethoxylate phosphate, 6% EO / PO block copolymer, 5% propylene glycol, 2% glycerol, 0.1% silicone defoamer, 0.1% xanthan gum, 0.1% sodium benzoate, and deionized water to make up the balance;

[0094] Preparation method: Same as Example 6.

[0095] Embodiment 8:

[0096] 20% compound of formula (I) · nicofluprole dispersible oil suspension (8+12)

[0097] Formula: 8% compound of formula (I), 12% nicofluprole,

[0098] 2.5% fatty alcohol polyoxyethylene ether, 15% castor oil polyoxyethylene ether, 0.3% organic bentonite, 0.2% organosilicon defoamer, 2% succinate sulfonate, and soybean oil to make up the balance;

[0099] Preparation method: according to the formula ratio, the active ingredients, surfactants and other functional additives are placed in a reaction kettle in sequence, oil is added and mixed evenly, and the dispersible oil suspension preparation of the composition of the present invention can be prepared by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0100] Indoor biological activity assay

[0101] Refer to agricultural industry standards:

[0102] "Guidelines for Indoor Bioassay Tests of Pesticides - Pesticides Part 6: Insect Dipping Method" (NY / T 1154.6-2006);

[0103] "Guidelines for Indoor Bioassay Tests of Pesticides - Pesticides Part 14: Leaf Dip Method" (NY / T 1154.14-2008);

[0104] "Guidelines for Indoor Bioassay Tests of Pesticides - Pesticides Part 7: Determination of Combined Action of Mixtures" (NY / T1154.7-2006), etc.

[0105] Specific indoor toxicity test:

[0106] 1) Lepidoptera larvae: a combination of leaf and insect dipping;

[0107] Test insect age: Use sensitive populations raised indoors for multiple generations, and select healthy and uniform second-instar Lepidoptera larvae.

[0108] Use tweezers to place fresh cabbage slices in the drug solution and immerse them for 10 seconds, then take them out. After the drug solution dries naturally, place the cabbage slices in a culture dish lined with moisturizing filter paper. Place 16 second-instar lepidopteran larvae in the drug solution for 5 seconds, absorb the excess drug solution with filter paper, and place the test insects in a culture dish with leaves immersed in the corresponding concentration.

[0109] Each treatment was repeated 4 times, and the blank treatment was used as the control. The treated test insects were placed in an artificial intelligence culture room at 26±1°C, a light period of L:D=16h:8h, and a relative humidity of 60% for breeding.

[0110] Investigation method: Investigate the mortality of test insects 48 hours after treatment with pesticides. The criterion for judging the death of test insects is obvious shrinkage of the insect body or inability to crawl normally after being pricked with a needle. Record the number of dead insects.

[0111] 2) Thysanoptera adults: glass roller tube method;

[0112] Test insect age: Use sensitive populations raised indoors for multiple generations, and select healthy and uniform female adults of the order Thysanoptera.

[0113] After the glass tube is evenly rolled with 250ul of the drug solution, the cabbage leaves are punched into 1.5cm diameter discs with a hole puncher. Then, fresh cabbage slices are placed in the drug solution with tweezers and immersed for 10s before being taken out. After the drug solution is naturally dried, the cabbage slices are placed in a glass tube. 20 female adults of Thysanoptera are sucked into the glass tube with an insect sucker and the tube is sealed with a 200-mesh gauze.

[0114] Each treatment was repeated 4 times, and the blank treatment was used as the control. The treated test insects were placed in an artificial intelligence culture room at 26±1°C, a light period of L:D=16h:8h, and a relative humidity of 60% for breeding.

[0115] Investigation method: Investigate the mortality of test insects 48 hours after treatment with pesticides. The criterion for judging the death of test insects is that they cannot crawl normally after being pricked with a needle. Record the number of dead insects.

[0116] Data statistics and analysis:

[0117] According to the survey data, the adjusted mortality rate of each treatment was calculated using the following formula, with the results retained to two decimal places:

[0118]

[0119] Where:

[0120] P——mortality rate, in percentage (%);

[0121] K——indicates the number of dead insects, in units of heads;

[0122] N——represents the total number of insects treated, in heads.

[0123]

[0124] Where:

[0125] P1——adjusted mortality rate, in percentage (%);

[0126] P t ——Treatment mortality rate, expressed as percentage (%);

[0127] P0——blank control mortality rate, in percentage (%).

[0128] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to formula (2); if the control mortality rate is greater than 20%, the test needs to be repeated.

[0129] The data can be processed by probability value analysis. The DPS statistical analysis system can be used to calculate the toxicity regression line, LC 50The activity of the test agent on the biological test material was evaluated by using the value, 95% confidence limit and correlation coefficient r.

[0130] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0131]

[0132] Where:

[0133] ATI——Actual Toxicity Index of Mixture;

[0134] S——LC of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0135] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0136] TTI=TI A *P A +TI B *P B

[0137] Where:

[0138] TTI – Theoretical Toxicity Index of Mixtures;

[0139] TI A ——A: toxicity index of the agent;

[0140] P A ——The percentage of agent A in the mixture, in percentage (%);

[0141] TI B ——Toxicity index of agent B;

[0142] P B ——The percentage of agent B in the mixture, in percentage (%).

[0143]

[0144] Where:

[0145] CTC – Co-toxicity coefficient;

[0146] ATI——Actual Toxicity Index of Mixture;

[0147] TTI - Theoretical Toxicity Index of Mixture.

[0148] The co-toxicity coefficient of the combination CTC≥120 shows a synergistic effect; CTC≤80 shows an antagonistic effect; 80<CTC<120 shows an additive effect.

[0149] Test results:

[0150] Combined toxicity of mixed pesticides to cabbage worms:

[0151] The results in Tables 1 and 2 show that the compound of formula (I) mixed with Indazapyroxamet and Nicofluprole has a good combined effect on Pieris rapae, and exhibits obvious synergistic effects at a certain mass ratio.

[0152] When the mass ratio of the compound of formula (I) to Indazapyroxamet is 1:32 to 30:1, the co-toxicity coefficient of the control effect against Pieris rapae is greater than 80, showing an additive or synergistic effect; when the mass ratio of the compound of formula (I) to Indazapyroxamet is 1:32 to 18:1, the co-toxicity coefficient of the control effect against Pieris rapae is greater than 120, showing a synergistic effect.

[0153] Table 1 Toxicity test results of different ratios of the compound of formula (I) and Indazapyroxamet against Pieris rapae

[0154]

[0155] When the mass ratio of the compound of formula (I) to Nicofluprole is 1:24 to 34:1, the co-toxicity coefficient of the protective effect against Pieris rapae is greater than 80, showing an additive or synergistic effect; when the mass ratio of the compound of formula (I) to Nicofluprole is 1:24 to 28:1, the co-toxicity coefficient of the protective effect against Pieris rapae is greater than 120, showing a synergistic effect.

[0156] Table 2 Toxicity test results of different ratios of compound of formula (I) and Nicofluprole to Pieris rapae

[0157]

[0158]

[0159] Combined toxicity of mixed pesticides to Thrips tabaci:

[0160] The results in Tables 3 and 4 show that the compound of formula (I) mixed with Flupyroxystrobin and Indazapyroxamet has a good combined effect on Thrips tabaci, and exhibits obvious synergistic effects at a certain mass ratio.

[0161] When the mass ratio of the compound of formula (I) to Flupyroxystrobin is 1:27 to 27:1, the co-toxicity coefficient of the control effect on Thrips tabaci is greater than 80, showing an additive or synergistic effect; when the mass ratio of the compound of formula (I) to Flupyroxystrobin is 1:18 to 27:1, the co-toxicity coefficient of the control effect on Thrips tabaci is greater than 120, showing a synergistic effect.

[0162] Table 3 Toxicity test results of different ratios of the compound of formula (I) and Flupyroxystrobin against Thrips tabaci

[0163]

[0164] When the mass ratio of the compound of formula (I) to Indazapyroxamet is 1:50 to 24:1, the co-toxicity coefficient of the control effect on Thrips tabaci is greater than 80, showing an additive or synergistic effect; when the mass ratio of the compound of formula (I) to Indazapyroxamet is 1:22 to 24:1, the co-toxicity coefficient of the control effect on Thrips tabaci is greater than 120, showing a synergistic effect.

[0165] Table 4 Toxicity test results of different ratios of the compound of formula (I) and Indazapyroxamet against Thrips tabaci

[0166]

[0167]

[0168] Field efficacy examples

[0169] Field trial 1

[0170] Experimental crops: Chinese chives (Dajingou);

[0171] Conditions of the experimental field: medium fertility, convenient irrigation and drainage, and sandy soil.

[0172] Test target: Leek thrips;

[0173] Test location: Qiantianbutou Village, Haiyang City, Yantai City;

[0174] Trial date: November 8, 2022;

[0175] Application method: In the test field, a backpack electric sprayer was used to apply pesticides manually according to the designed plan. The whole plant was sprayed first, and then the back of the leaves was sprayed. The application machine was a 3WBS-16 backpack electric sprayer with a spray volume of 30L / mu.

[0176] Experimental environment: All experimental plots had uniform cultivation conditions, the same fertilizer and water management level, good water and fertilizer conditions, and sticky loam;

[0177] Test drug:

[0178] Table 5 Field test design and dosage

[0179]

[0180] Test method: Apply the pesticide once at the early stage of leek thrips infestation (small white spots appear on the leaves).

[0181] Plot size and repetition: random block arrangement, plot size 25m 2 , each treatment was repeated 4 times.

[0182] Survey method: Randomly sample five points in each plot, use the clapping method, randomly survey 5 clumps (20 plants) at each point, clap them together 3 times, count the number of thrips shaken into the white porcelain plate, investigate the insect base number before applying the pesticide, and investigate the number of residual insects 1d and 7d after applying the pesticide.

[0183] Calculation formula and data analysis:

[0184]

[0185] Field efficacy test results:

[0186] The field control effects of each agent on leek thrips are shown in Table 6. It can be seen from the table that 1 day after the application, 25% formula (Ⅰ) compound·flupyroxystrobin suspension (15+10) and 28% formula (Ⅰ) compound·indazapyroxamet suspension (8+20) showed good quick-acting properties on leek thrips, and the dosage of the active ingredient was 10 g ai / hm 2 The control effects were 87.35% and 86.19% respectively. With the increase of time, 7 days after application, the control effects of 25% formula (Ⅰ) compound·flupyroxystrobin suspension (15+10) and 28% formula (Ⅰ) compound·indazapyroxamet suspension (8+20) on leek thrips increased, and the dosage of active ingredient was 10g ai / hm 2 The protective effects were 92.27% and 91.44% respectively, which were significantly higher than the control single-dose and lasted longer.

[0187] Table 6 Field control effect of different treatment agents on leek thrips

[0188]

[0189] Note: The above insect population base and control efficacy data are the average of 4 replicates, and the values ​​are rounded to two decimal places. Different letters after the control efficacy data in the same column indicate significant differences (P < 0.05).

[0190] Field trial 2

[0191] Test crops: Chinese cabbage (Green Show);

[0192] Test target: Pieris rapae;

[0193] Test location: Chinese cabbage planting test field in Lijia Village, Zhucheng, Weifang City, Shandong Province;

[0194] Trial date: October 6, 2022;

[0195] Application method: Use a backpack electric sprayer in the test field to apply pesticides manually according to the designed plan. First spray the whole plant, and then focus on spraying the back of the leaves.

[0196] Experimental environment: The terrain is flat, the soil is loam, and the fertility is medium. The cultivation conditions of all experimental plots are uniform and consistent, and the same fertilizer and water management level is adopted. The water and fertilizer conditions are relatively good, and the soil is sticky loam;

[0197] Test drug:

[0198] Table 7 Field test design and dosage

[0199]

[0200] Test method: Apply the pesticide once during the peak period of cabbage looper occurrence (mostly 2nd to 4th instar).

[0201] Plot size and repetition: random block arrangement, plot size 20m 2 , each treatment was repeated 4 times.

[0202] Survey method: Randomly sample five points in each plot, hang a sign on 4 plants at each point, investigate the number of cabbage loopers on all the cabbages, investigate the base number of insects before applying pesticides, and investigate the number of remaining insects 3d and 7d after applying pesticides.

[0203] Calculation formula and data analysis:

[0204]

[0205] Field efficacy test results: The field control effects of various agents on Chinese cabbage worms are shown in Table 8. It can be seen from the table that 3 days after the application, 1.4% of the compound of formula (Ⅰ)·indazapyroxamet emulsion (1+0.4) and 20% of the compound of formula (Ⅰ)·nicofluprole dispersible oil suspension (8+12) showed good rapid effect on cabbage worms, and the dosage of the active ingredient was 15 g a.i / hm 2 The control effects were 84.17% and 85.28% respectively. With the increase of time, 7 days after application, the control effects of 1.4% compound of formula (Ⅰ)·indazapyroxamet emulsion (1+0.4) and 20% compound of formula (Ⅰ)·nicofluprole dispersible oil suspension (8+12) on Pieris rapae increased, and the dosage of active ingredient was 15g ai / hm 2 The protective effects were 89.63% and 90.20% respectively, which were significantly higher than the control single-dose and lasted longer.

[0206] Table 8 Field control effect of different treatment agents on Chinese cabbage worm

[0207]

[0208] Note: The above insect population base and control efficacy data are the average of 4 replicates, and the values ​​are rounded to two decimal places. Different letters after the control efficacy data in the same column indicate significant differences (P < 0.05).

[0209] Safety: During the test period and later observations, the Chinese cabbage grew normally and no obvious pesticide damage was observed.

[0210] The insecticide composition or the preparation obtained by compounding the present invention has significant control effect, and is superior to a single agent in delaying the generation of drug resistance and prolonging drug retention. In addition, no compound agent was found to cause phytotoxicity to crops in the test, indicating that the insecticide composition or preparation obtained can reduce production costs and use costs and is safe for crops when the insecticide synergistic synergy is improved.

[0211] Although the present invention has been described in detail above with general instructions and specific implementation schemes, it is obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. An insecticidal composition, It is characterized in that The active ingredient A and the active ingredient B are included, wherein the active ingredient A is a compound of formula (I) with the following structural formula: The active ingredient B is Flupyroxystrobin, Either Indazapyroxamet or Nicofluprole.

2. The insecticidal composition according to claim 1, Features: The mass ratio of the active ingredient A to the active ingredient B is 1:50 to 34:1; Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:24 to 28:

1.

3. The insecticidal composition according to claim 1, Features: The mass ratio of the compound of formula (I) to Flupyroxystrobin is 1:27 to 27:1; the mass ratio of the compound of formula (I) to Indazapyroxamet is 1:50 to 30:1; the mass ratio of the compound of formula (I) to Nicofluprole is 1:24 to 34:

1.

4. The insecticidal composition according to claim 1, Features: The mass ratio of the compound of formula (I) to Flupyroxystrobin is 1:18 to 27:1; the mass ratio of the compound of formula (I) to Indazapyroxamet is 1:32 to 24:1; the mass ratio of the compound of formula (I) to Nicofluprole is 1:24 to 28:

1.

5. The insecticidal composition according to claim 1, Features: Based on the total mass of the insecticide composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the insecticide composition is 0.5% to 90%; Preferably, the sum of the contents of the active ingredient A and the active ingredient B in the insecticide composition is 1% to 85%.

6. The insecticidal composition according to claim 1, Features: In addition to the active ingredients, the insecticide composition also includes agriculturally acceptable auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists or carriers.

7. The insecticidal composition according to claim 6, Features: The insecticide composition is prepared into a formulation form permitted in agriculture, and the formulation form is selected from a solid formulation and / or a liquid formulation; Preferably, the solid preparation is selected from wettable powders and water dispersible granules; The liquid preparation is selected from emulsifiable concentrates, aqueous emulsions, microemulsions, suspensions, suspoemulsions, and dispersible oil suspensions.

8. Use of the insecticide composition according to any one of claims 1 to 7 in preventing and controlling agricultural and forestry pests and sanitary pests.

9. The use according to claim 8, Features: The agricultural and forestry pests and sanitary pests are Lepidoptera and Thysanoptera pests; Preferably, the lepidopteran pests are cabbage looper, diamondback moth, beet armyworm, Spodoptera litura, Spodoptera litura, Chilo suppressalis, and cotton bollworm; the thysanoptera pests are western flower thrips, tobacco thrips, palm thrips, and watermelon thrips.

10. The insecticidal composition according to claim 1, Features: The insecticide composition is applied to the pests to be controlled or the medium where they grow in an effective dose.

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