High-moisture-retention antioxidant lipstick containing rice bran polysaccharide and preparation method of high-moisture-retention antioxidant lipstick

Abstract

The invention discloses a high-moisturizing antioxidant lipstick containing rice bran polysaccharide and a preparation method of the high-moisturizing antioxidant lipstick, and belongs to the technical field of lipstick preparation. The lipstick is prepared from the following raw materials in parts by weight: 31.0-46.7 parts of natural vegetable oil; 14.0 to 27.0 parts of animal and plant wax; 1.0 to 5.0 parts of rice bran polysaccharide; 1.0 to 5.0 parts of toner; 0.8 to 4.0 parts of squalane; 0.9 to 3.7 parts of vitamin E; 0.7 to 2.8 parts of rosemary essential oil; and 0.5 to 1.2 parts of essence. The rice bran polysaccharide is extracted from rice bran and applied to the lipstick product, the invention discloses an extraction process of the rice bran polysaccharide, the rice bran polysaccharide extracted by the extraction process has excellent oxidation resistance through detection, and after the lipstick product is prepared, the oxidation resistance of the rice bran polysaccharide is greatly improved through detection. The lipstick added with the rice bran polysaccharide prepared by the invention has more excellent moisturizing performance.

Description

Technical Field

[0001] The invention belongs to the technical field of lipstick preparation, and particularly relates to a high-moisturizing and antioxidant lipstick containing rice bran polysaccharide and a preparation method thereof. Background Art

[0002] Lipstick is a common cosmetic that is widely used in daily life and special occasions. The moisturizing and antioxidant properties of lipstick have always been a hot topic for consumers. The reason is that highly moisturizing lipstick can absorb and lock in moisture, keep the lips moist for a long time, and reduce the occurrence of dryness and peeling problems; antioxidant lipstick can prevent the lips from being damaged by free radicals. Free radicals are the culprit that causes lip aging and pigmentation, and lipstick with antioxidant properties can neutralize the effects of free radicals and keep the lips from damage.

[0003] In the prior art, many improvements have been made to the moisturizing and antioxidant properties of lipsticks:

[0004] Chinese patent CN116421539A discloses a lipstick with a natural composite formula and a preparation method thereof. The lipstick in the invention patent is composed of the following raw materials: natural animal and plant oils and fats (squalane, sea buckthorn oil, jojoba oil, almond oil, olive oil, etc.), natural waxes (beeswax, candelilla wax, palm wax, etc.), natural pigments, thickeners, vitamin E, natural colloids, antibacterial extracts (rosemary essential oil extracts, etc.), emulsifiers, humectants, color protectants, natural plant essential oils, polypeptides and small molecules. The invention aims to establish an emulsifying, anti-oxidation, antiseptic, thickening and moisturizing system to improve the problems of low color development, short shelf life, and greasy and non-moisturizing of natural ingredient lipstick. The antioxidants used in the invention patent include vitamin E, vitamin C, citric acid and flavonoid natural antioxidants, which are still common antioxidant components and have not made significant improvements to the antioxidant properties of lipstick products.

[0005] Chinese patent CN110840789B discloses a color-changing lipstick containing plant pigments and a preparation method thereof. The raw materials for preparing the lipstick in this invention patent include: natural wax (beeswax, candelilla wax, carnauba wax), natural plant oils (plant squalane, olive oil, jojoba seed oil, etc.), plant pigments, antioxidants, and color-changing powder. The lipstick of this invention has the effects of moisturizing and sensing the pH value of lips or saliva to gradually change. The antioxidant in this invention patent includes any one of pentaerythritol tetra(di-tert-butylhydroxyhydrocinnamate), butylated hydroxytoluene or tocopherol, or a combination of at least two of them. Among them, butylated hydroxytoluene may damage the human liver and may also cause cancer.

[0006] Rice bran, as a by-product of rice processing, has attracted extensive attention from domestic and foreign researchers in recent years. The polysaccharides extracted from rice bran have multiple biological activities due to the particularity of their structure. The present invention aims to extract rice bran polysaccharides from rice bran and apply them to the development of highly moisturizing and antioxidant lipstick. The present invention is based on the prior art and aims to provide a new improvement idea. Summary of the invention

[0007] The invention provides a highly moisturizing and antioxidant lipstick containing rice bran polysaccharide and a preparation method thereof, so as to solve the technical problem that the means for improving the moisturizing and antioxidant properties of the existing lipsticks are relatively single.

[0008] One object of the present invention is to provide a highly moisturizing and antioxidant lipstick containing rice bran polysaccharide;

[0009] Another object of the present invention is to provide a method for preparing the lipstick.

[0010] The purpose of the present invention can be achieved through the following technical solutions:

[0011] A highly moisturizing and antioxidant lipstick containing rice bran polysaccharide, prepared from the following raw materials in parts by weight:

[0012]

[0013] Furthermore, the natural vegetable oil is a mixture of olive oil, sweet almond oil, jojoba oil and sea buckthorn oil in a mass ratio of 3.0-7.0g:16.4-20.0g:7.0-10.5g:4.6-9.2.

[0014] Furthermore, the animal and plant wax is a mixture of white beeswax, candelilla wax and palm wax in a dosage ratio of 8.0-12.0g:5.0-10.0g:1.0-5.0g.

[0015] Furthermore, the extraction of rice bran polysaccharides comprises the following steps:

[0016] Step S1, soaking rice bran in petroleum ether and heating to 80-90° C., stirring and refluxing for 4 hours, and then heating at a constant temperature to separate the petroleum ether to obtain defatted rice bran, and air-drying the defatted rice bran, crushing it through a 60-mesh sieve, and obtaining defatted rice bran dry powder;

[0017] Step S2, adding distilled water to the defatted rice bran powder, reflux leaching in a hot water bath at a water bath temperature of 100° C. and an extraction time of 2 h, centrifuging after the extraction is complete, combining the supernatants, and concentrating to 50 mL in a vacuum rotation to obtain a concentrated solution, adding ethanol to the concentrated solution until the volume concentration of ethanol is 80%, and obtaining a mixed solution, placing the mixed solution at 4° C. for 12 h, and then centrifuging at a speed of 5000 rpm for 10 min to obtain a precipitate, adding deionized water to the precipitate, stirring at a constant temperature until the precipitate is completely dissolved, and then separating the residual ethanol at 80-90° C. to obtain a rice bran crude polysaccharide aqueous solution, freeze-drying the rice bran crude polysaccharide aqueous solution to obtain rice bran crude polysaccharide, adding pure water to the rice bran crude polysaccharide, stirring until the rice bran crude polysaccharide is completely dissolved, and then adding α-amylase, and dripping The pH value of the solution is adjusted to 5.8-6.0 with a 0.1M citric acid-sodium citrate buffer solution, and the solution is placed in a water bath at 60°C for enzymolysis for 4-6 hours, and then 10-12 mg of saccharifying enzyme is added. After the enzymolysis is continued for 2-3 hours, the solution is heated to 100°C for constant temperature enzyme inactivation for 5 minutes, and then centrifuged at 5000 rpm for 10 minutes, the supernatant is taken, and 15% of the total volume of the supernatant TCA with a mass concentration of 60 g / L is added to the supernatant with stirring, and after stirring for 10 minutes, the solution is placed at 4°C for 30-60 minutes, the protein is filtered out, and then centrifuged at 5000 rpm for 10 minutes, the supernatant is taken, and the pH value of the system is adjusted to 7.0-7.2 with anhydrous sodium carbonate to obtain a mixed solution, and the mixed solution is dialyzed and purified with ultrapure water for 2 days. After the dialysis is completed, the solution is freeze-dried to obtain non-starch rice bran polysaccharides.

[0018] Furthermore, the dosage ratio of rice bran and petroleum ether in step S1 is 100:500 mL.

[0019] Furthermore, in step S2, the dosage ratio of the defatted rice bran powder, distilled water, deionized water, pure water, α-amylase, and saccharifying enzyme is 10.0 g: 180-200 mL: 100 mL: 80 mL: 10-12 mg: 10-12 mg.

[0020] Furthermore, in step S2, the enzyme amount of the α-amylase is ≥6000 U / mg; the enzyme amount of the saccharifying enzyme is ≥3500 U / mg.

[0021] Furthermore, the preparation method of the lipstick comprises the following steps:

[0022] The above raw materials are weighed according to the mass ratio, natural vegetable oil and color powder are added to a conical flask, ultrasonication is performed until the solid is completely dissolved, and then animal and plant waxes are added, the conical flask is placed in a 100°C water bath for heating, and the materials in the conical flask are completely melted, and then squalane, vitamin E, essence and rosemary essential oil are added to the conical flask, stirring at a constant temperature for 8-10 minutes, and stirring is stopped to obtain a material, and the material is injected into a lipstick mold, shaken for 15-20 minutes, and placed in a refrigerator at 4-10°C for refrigeration for 30 minutes, taken out, demoulded, and piped to obtain a highly moisturizing and antioxidant lipstick containing rice bran polysaccharide.

[0023] Beneficial effects of the present invention:

[0024] The invention discloses a highly moisturizing and anti-oxidation lipstick containing rice bran polysaccharide, which is composed of the following raw materials: natural plant oil (olive oil, sweet almond oil, jojoba oil, sea buckthorn fruit oil), animal and plant wax (white beeswax, candelilla wax, palm wax), rice bran polysaccharide, color powder, squalane, vitamin E, rosemary essential oil, and essence. The invention extracts rice bran polysaccharide from rice bran and applies it in lipstick products. The invention also discloses an extraction process of rice bran polysaccharide. After testing, the rice bran polysaccharide extracted by the extraction process of the invention has excellent anti-oxidation performance. After preparing the lipstick product, after testing, the lipstick to which the rice bran polysaccharide prepared by the invention is added has more excellent moisturizing performance.

[0025] In the highly moisturizing and antioxidant lipstick provided by the present invention, the moisturizing function is achieved by the comprehensive effects of rice bran polysaccharides, squalane, vitamin E, etc., and finally the roughness of the lip skin is reduced, which helps to improve the texture of the lip skin and enhance the elasticity of the lip skin. The antioxidant function is achieved by the comprehensive effects of rice bran polysaccharides, vitamin E, etc., and finally the effect of neutralizing free radicals is achieved to keep the lips from damage. DETAILED DESCRIPTION

[0026] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] Example 1

[0028] Extraction of rice bran polysaccharides:

[0029] Take 100g rice bran (purchased from Changsha Lieyanyun Trading Co., Ltd.) and soak it in 500mL petroleum ether (spectrally pure; boiling point 60-90°C; purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.) and heat it to 80°C, stir and reflux for 4h, and carry out degreasing treatment. After the degreasing treatment, continue constant temperature heating to separate petroleum ether, which can be recycled and reused. After separation, obtain defatted rice bran, place the defatted rice bran in a fume hood to air-dry, then pulverize through a 60-mesh sieve to obtain defatted rice bran dry powder, seal and preserve for standby use;

[0030] 10.0 g defatted rice bran powder was accurately weighed, and distilled water was added to the defatted rice bran powder as an extraction solvent at a solid-liquid ratio of 1:18 g / mL. The mixture was refluxed and extracted twice in a hot water bath at a water bath temperature of 100° C. and an extraction time of 2 h. After the extraction was completed, the mixture was centrifuged, the supernatant was combined, and the mixture was concentrated to 50 mL in a vacuum rotary manner to obtain a concentrated solution. Ethanol was added to the concentrated solution until the volume concentration of ethanol was 80% to obtain a mixed solution. The mixed solution was placed at 4° C. for 12 h, and then centrifuged at a rate of 5000 rpm for 10 min to obtain a precipitate. 100 mL of deionized water was added to the precipitate, and the mixture was stirred at a constant temperature until the precipitate was completely dissolved. The mixture was then placed at 80° C. to separate the residual ethanol to obtain a crude rice bran polysaccharide aqueous solution. The crude rice bran polysaccharide aqueous solution was then placed in a freeze dryer for freeze drying to obtain crude rice bran polysaccharides. 80 mL of pure water was added to the crude rice bran polysaccharides, and the mixture was stirred until the crude rice bran polysaccharides were completely dissolved. 10 mg of α-amylase (≥60 00U / mg, Shanghai Aladdin Biochemical Technology Co., Ltd.), add 0.1M citric acid-sodium citrate buffer solution to adjust the pH to 5.8, place it in a 60℃ water bath for enzymolysis for 4h, then add 10mg of saccharifying enzyme (≥3500U / mg, Shanghai Yuanye Biotechnology Co., Ltd.), continue enzymolysis for 2h, then heat to 100℃ and inactivate the enzyme for 5min, then centrifuge at 5000rpm for 10min, take the supernatant, stir and add 15% of the total volume of the supernatant TCA (mass concentration of 60g / L, trichloroacetic acid) to the supernatant, stir for 10min, place it at 4℃ and let it stand for 30min, the protein is fully precipitated, filter to remove the protein, and then centrifuge at 5000rpm for 10min, take the supernatant, adjust the system pH to 7.0 with anhydrous sodium carbonate to obtain a mixed solution, and dialysis the mixed solution with ultrapure water for 2d. After the dialysis is completed, freeze-drying is performed to obtain non-starch rice bran polysaccharides.

[0031] Example 2

[0032] Extraction of rice bran polysaccharides:

[0033] Take 100g rice bran (purchased from Changsha Lieyanyun Trading Co., Ltd.) and soak it in 500mL petroleum ether (spectrally pure; boiling point 60-90°C; purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.) and heat it to 85°C, stir and reflux for 4h, carry out degreasing treatment, after degreasing treatment, continue constant temperature heating to separate petroleum ether, petroleum ether can be recycled and reused, after separation, obtain defatted rice bran, place defatted rice bran in a fume hood to air-dry, then pulverize through a 60-mesh sieve to obtain defatted rice bran dry powder, seal and preserve, and set aside;

[0034] 10.0 g defatted rice bran powder was accurately weighed, and distilled water was added to the defatted rice bran powder as an extraction solvent at a solid-liquid ratio of 1:19 g / mL. The mixture was refluxed and extracted twice in a hot water bath at a water bath temperature of 100° C. and an extraction time of 2 h. After the extraction was completed, the mixture was centrifuged, the supernatant was combined, and the mixture was concentrated to 50 mL by vacuum rotation to obtain a concentrated solution. Ethanol was added to the concentrated solution until the volume concentration of ethanol was 80% to obtain a mixed solution. The mixed solution was placed at 4° C. for 12 h, and then centrifuged at a rate of 5000 rpm for 10 min to obtain a precipitate. 100 mL of deionized water was added to the precipitate, and the mixture was stirred at a constant temperature until the precipitate was completely dissolved. The mixture was then placed at 85° C. to separate the residual ethanol to obtain a crude rice bran polysaccharide aqueous solution. The crude rice bran polysaccharide aqueous solution was then placed in a freeze dryer for freeze drying to obtain crude rice bran polysaccharides. 80 mL of pure water was added to the crude rice bran polysaccharides, and the mixture was stirred until the crude rice bran polysaccharides were completely dissolved. 11 mg of α-amylase (≥60 00U / mg, Shanghai Aladdin Biochemical Technology Co., Ltd.), add 0.1M citric acid-sodium citrate buffer solution to adjust the pH to 5.9, place it in a 60℃ water bath for enzymolysis for 5h, then add 11mg of saccharifying enzyme (≥3500U / mg, Shanghai Yuanye Biotechnology Co., Ltd.), continue enzymolysis for 3h, then heat to 100℃ and inactivate the enzyme for 5min, then centrifuge at 5000rpm for 10min, take the supernatant, stir and add 15% of the total volume of the supernatant TCA (mass concentration of 60g / L, trichloroacetic acid) to the supernatant, stir for 10min, place it at 4℃ and let it stand for 50min, the protein is fully precipitated, filter to remove the protein, and then centrifuge at 5000rpm for 10min, take the supernatant, adjust the system pH to 7.1 with anhydrous sodium carbonate to obtain a mixed solution, and dialysis the mixed solution with ultrapure water for 2d. After the dialysis is completed, freeze-drying is performed to obtain non-starch rice bran polysaccharides.

[0035] Example 3

[0036] Extraction of rice bran polysaccharides:

[0037] Take 100g rice bran (purchased from Changsha Lieyanyun Trading Co., Ltd.) and soak it in 500mL petroleum ether (spectrally pure; boiling point 60-90°C; purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.) and heat it to 90°C, stir and reflux for 4h, carry out degreasing treatment, after degreasing treatment, continue constant temperature heating to separate petroleum ether, petroleum ether can be recycled and reused, after separation, obtain defatted rice bran, place defatted rice bran in a fume hood to air-dry, then pulverize through a 60-mesh sieve to obtain defatted rice bran dry powder, seal and preserve, and set aside;

[0038] 10.0 g defatted rice bran powder was accurately weighed, and distilled water was added to the defatted rice bran powder as an extraction solvent at a solid-liquid ratio of 1:20 g / mL. The mixture was refluxed and extracted twice in a hot water bath at a water bath temperature of 100° C. and an extraction time of 2 h. After the extraction was completed, the mixture was centrifuged, the supernatant was combined, and the mixture was concentrated to 50 mL by vacuum rotation to obtain a concentrated solution. Ethanol was added to the concentrated solution until the volume concentration of ethanol was 80% to obtain a mixed solution. The mixed solution was placed at 4° C. for 12 h, and then centrifuged at a rate of 5000 rpm for 10 min to obtain a precipitate. 100 mL of deionized water was added to the precipitate, and the mixture was stirred at a constant temperature until the precipitate was completely dissolved. The mixture was then placed at 90° C. to separate the residual ethanol to obtain a rice bran crude polysaccharide aqueous solution. The rice bran crude polysaccharide aqueous solution was then placed in a freeze dryer for freeze drying to obtain rice bran crude polysaccharides. 80 mL of pure water was added to the rice bran crude polysaccharides, and the rice bran crude polysaccharides were stirred until the rice bran crude polysaccharides were completely dissolved. 12 mg of α-amylase (≥60 00U / mg, Shanghai Aladdin Biochemical Technology Co., Ltd.), add 0.1M citric acid-sodium citrate buffer solution to adjust the pH to 6.0, place it in a 60℃ water bath for enzymolysis for 6h, then add 12mg of saccharifying enzyme (≥3500U / mg, Shanghai Yuanye Biotechnology Co., Ltd.), continue enzymolysis for 3h, then heat to 100℃ and inactivate the enzyme for 5min, then centrifuge at 5000rpm for 10min, take the supernatant, stir and add 15% of the total volume of the supernatant TCA (mass concentration of 60g / L, trichloroacetic acid) to the supernatant, stir for 10min, place it at 4℃ and let it stand for 60min, the protein is fully precipitated, filter to remove the protein, and then centrifuge at 5000rpm for 10min, take the supernatant, adjust the system pH to 7.2 with anhydrous sodium carbonate to obtain a mixed solution, and dialysis the mixed solution with ultrapure water for 2d. After the dialysis is completed, freeze-drying is performed to obtain non-starch rice bran polysaccharides.

[0039] The rice bran polysaccharides prepared in Examples 1 to 3 were tested for their antioxidant properties. The antioxidant property testing process is as follows, and the test results are shown in Table 1:

[0040] Using deionized water as solvent, the three groups of rice bran polysaccharides were prepared into polysaccharide solutions with different mass concentrations (0.8 mg / mL, 1.0 mg / mL, and 1.2 mg / mL) for antioxidant capacity determination. Each test was repeated three times and the results were averaged.

[0041] Determination of DPPH free radical scavenging rate:

[0042] Accurately weigh 19.75 mg of DPPH standard, dissolve it with anhydrous ethanol and make up to volume in a 500 mL volumetric flask to prepare a 0.1 mM DPPH-ethanol solution, and store it in a refrigerator at 4°C away from light. Take 1 mL of polysaccharide solution of different concentrations, add 2 mL of DPPH-ethanol solution, shake well, react at room temperature away from light for 30 minutes, and measure its absorbance (As) at 517 nm. Deionized water replaces the polysaccharide solution as a blank control (Ac), and Vc is used as a positive control. The DPPH clearance formula is as follows:

[0043] DPPH free radical scavenging rate (%) = [(A c -A s ) / A c ]×100

[0044] Table 1 Test results of antioxidant properties of rice bran polysaccharides

[0045]

[0046] It can be seen from Table 1 that the rice bran polysaccharides extracted by the extraction process in Examples 1-3 of the present invention have excellent antioxidant properties.

[0047] Example 4

[0048] A highly moisturizing and antioxidant lipstick containing rice bran polysaccharide, prepared from the following raw materials in parts by weight:

[0049]

[0050] Among them, the raw materials of each raw material: olive oil: Lidomai Shou (Zhejiang) E-Commerce Co., Ltd.; sweet almond oil: Yiwu Junbo Trading Co., Ltd.; sea buckthorn fruit oil: Suifenhe Russian Honey Russian Goods Store; jojoba oil: Yiwu Junbo Trading Co., Ltd.; color powder: Suqian Dongyuda Trading Co., Ltd.; palm wax: Yiwu Lanyan Trading Co., Ltd.; white beeswax: Yiwu Lanyan Trading Co., Ltd.; candelilla wax: Yiwu Lanyan Trading Co., Ltd.; squalane: Yiwu Lanyan Trading Co., Ltd.; vitamin E: Lu'an Ping'an Pharmaceutical Chain Co., Ltd.; flavor: Yiwu Lanyan Trading Co., Ltd.; rosemary essential oil: Yiwu Lanyan Trading Co., Ltd.

[0051] Specifically, the preparation method of the highly moisturizing and antioxidant lipstick containing rice bran polysaccharide comprises the following steps:

[0052] The above raw materials are weighed according to the mass ratio, olive oil, sweet almond oil, jojoba oil, sea buckthorn fruit oil and color powder are added to a conical flask, ultrasonic treatment is performed until the solid is completely dissolved, white beeswax, candelilla wax and palm wax are added, the conical flask is placed in a 100°C water bath for heating, and the materials in the conical flask are completely melted, squalane, vitamin E, essence and rosemary essential oil are added to the conical flask, and the mixture is stirred at a constant temperature for 8 minutes, and the stirring is stopped to obtain the material, and the material is injected into a lipstick mold, shaken for 15 minutes, and refrigerated in a refrigerator at 4°C for 30 minutes, taken out, demoulded, and piped to obtain a highly moisturizing and antioxidant lipstick containing rice bran polysaccharides.

[0053] Example 5

[0054] A highly moisturizing and antioxidant lipstick containing rice bran polysaccharide, prepared from the following raw materials in parts by weight:

[0055]

[0056] Wherein, the raw materials of each raw material are the same as those in Example 4.

[0057] Specifically, the preparation method of the highly moisturizing and antioxidant lipstick containing rice bran polysaccharide comprises the following steps:

[0058] The above raw materials are weighed according to the mass ratio, olive oil, sweet almond oil, jojoba oil, sea buckthorn fruit oil and color powder are added to a conical flask, ultrasonication is performed until the solid is completely dissolved, white beeswax, candelilla wax and palm wax are added, the conical flask is placed in a 100°C water bath for heating, and the materials in the conical flask are completely melted, squalane, vitamin E, essence and rosemary essential oil are added to the conical flask, stirring at a constant temperature for 9 minutes, stopping stirring to obtain a material, injecting the material into a lipstick mold, shaking for 18 minutes, putting it in a refrigerator at 8°C for refrigeration for 30 minutes, taking it out, demoulding, and pipe-packing to obtain a highly moisturizing and antioxidant lipstick containing rice bran polysaccharides.

[0059] Example 6

[0060] A highly moisturizing and antioxidant lipstick containing rice bran polysaccharide, prepared from the following raw materials in parts by weight:

[0061]

[0062] Wherein, the raw materials of each raw material are the same as those in Example 4.

[0063] Specifically, the preparation method of the highly moisturizing and antioxidant lipstick containing rice bran polysaccharide comprises the following steps:

[0064] The above raw materials are weighed according to the mass ratio, olive oil, sweet almond oil, jojoba oil, sea buckthorn fruit oil and color powder are added to a conical flask, ultrasonication is performed until the solid is completely dissolved, white beeswax, candelilla wax and palm wax are added, the conical flask is placed in a 100°C water bath for heating, and the materials in the conical flask are completely melted, squalane, vitamin E, essence and rosemary essential oil are added to the conical flask, and the mixture is stirred at a constant temperature for 10 minutes, and the stirring is stopped to obtain the material, and the material is injected into a lipstick mold, shaken for 20 minutes, and refrigerated in a refrigerator at 10°C for 30 minutes, taken out, demoulded, and piped to obtain a highly moisturizing and antioxidant lipstick containing rice bran polysaccharide.

[0065] Comparative Example 1

[0066] Comparative Example 1 is the control group of Example 5, in which the rice bran polysaccharide prepared in Example 2 in Example 5 is removed, and the remaining raw materials and the preparation method thereof remain unchanged, and finally a highly moisturizing and antioxidant lipstick is obtained.

[0067] The lipsticks prepared in Examples 4 to 6 and Comparative Example 1 were subjected to performance tests. The performance test process is as follows, and the test results are shown in Table 2:

[0068] (1) Texture: Take a sample, unscrew it completely, and observe it under mild indoor conditions without direct sunlight to see whether the lipstick paste has a smooth surface, no pores, and no impurities visible to the naked eye.

[0069] (2) Color: Take the sample, unscrew it completely, and observe it indoors under a mild, non-sunlight environment to see if the color is uniform and bright, and if there is no lumps or color changes in the color powder.

[0070] (3) Odor: Take a sample, twist it out completely, and use your sense of smell to determine whether it is irritating.

[0071] (4) Stickiness: Apply the sample to your finger and determine the stickiness.

[0072] (5) Extensibility: Apply the sample on the same paper and compare whether the application is even without lumps and whether it is easy to spread.

[0073] (6) Moisturizing degree: First apply the lipstick on your finger to determine the moisturizing degree. Then take a piece of blotting paper and use a pencil to draw a rectangular area of ​​2.0 cm x 3.0 cm on the blotting paper. Apply the test lipstick completely and evenly within the drawn rectangle. Leave it overnight. The next day, look at the lipstick on the blotting paper again. The wider the range of the lip line drawn by pencil, the better the moisturizing degree.

[0074] (7) Heat resistance: Place the prepared lipstick sample in a constant temperature box set at 45°C (temperature control accuracy ±1°C). After 30 minutes, observe whether the paste has any bending, softening, oil leakage or breakage.

[0075] (8) Moisturizing: Before starting the test, the three participants need to clean the inner front of both arms and wipe them carefully with dry tissue paper without leaving any moisture. Before the test, they need to sit in the room for more than 30 minutes. During this period, they are prohibited from drinking water or other beverages. They should expose their forearms and stay relaxed. Apply lipstick on the outside of the left forearm, evenly apply three layers in an area of ​​2cm×3cm. Select the same area on the outside of the right forearm to apply the uncoated skin area with lipstick as a control. Each area is measured in parallel 5 times, and the average value is used as the test value. The test data 15 minutes after applying lipstick is recorded until all tests are completed. The test samples are randomly distributed on the left and right arms. Use a capacitive skin moisture meter to test and calculate the skin moisture content before and 15 minutes after applying cosmetics, and finally calculate the MMV value:

[0076] Skin moisture content change rate = [moisture content after use - moisture content before use / moisture content before use] × 100%.

[0077] Table 2 Lipstick performance test results

[0078] project Example 4 Example 5 Example 6 Comparative Example 1 texture More uniform texture Uniform texture More uniform texture Uniform texture Color Strong gloss Strong gloss Average glossiness Strong gloss Stickiness Mild stickiness No stickiness Mild stickiness No stickiness Ductility better good better good Moisture Higher high Higher Higher Heat resistant Slight melting bend No Melting Slight melting bend No Melting MMV value / % 39.2 42.5 41.8 28.8

[0079] It can be seen from Table 2 that, compared with the data of Example 5 and Comparative Example 1, the rice bran polysaccharide prepared by the present invention has better moisturizing performance.

[0080] It should be noted that, in this article, terms such as "include", "comprises" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or equipment that includes a series of elements includes not only those elements, but also includes other elements not explicitly listed, or also includes elements inherent to such process, method, article or equipment.

[0081] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the invention, and the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A highly moisturizing and antioxidant lipstick containing rice bran polysaccharides, characterized in that: It is prepared from the following raw materials in parts by weight:

2. The highly moisturizing and antioxidant lipstick containing rice bran polysaccharides according to claim 1, characterized in that: The natural vegetable oil is a mixture of olive oil, sweet almond oil, jojoba oil and sea buckthorn fruit oil in a mass ratio of 3.0-7.0 g: 16.4-20.0 g: 7.0-10.5 g: 4.6-9.

2.

3. The highly moisturizing and antioxidant lipstick containing rice bran polysaccharides according to claim 1, characterized in that: The animal and plant wax is prepared by mixing white beeswax, candelilla wax and palm wax in a dosage ratio of 8.0-12.0 g: 5.0-10.0 g: 1.0-5.0 g.

4. The highly moisturizing and antioxidant lipstick containing rice bran polysaccharides according to claim 1, characterized in that: The extraction of rice bran polysaccharide comprises the following steps: Step S1, soaking rice bran in petroleum ether and heating to 80-90° C., stirring and refluxing for 4 hours, and then heating at a constant temperature to separate the petroleum ether to obtain defatted rice bran, and air-drying the defatted rice bran, crushing it through a 60-mesh sieve, and obtaining defatted rice bran dry powder; Step S2, adding distilled water to the defatted rice bran powder, reflux leaching in a hot water bath at a water bath temperature of 100° C. and an extraction time of 2 h, centrifuging after the extraction is complete, combining the supernatants, and concentrating to 50 mL in a vacuum rotation to obtain a concentrated solution, adding ethanol to the concentrated solution until the volume concentration of ethanol is 80%, and obtaining a mixed solution, placing the mixed solution at 4° C. for 12 h, and then centrifuging at a speed of 5000 rpm for 10 min to obtain a precipitate, adding deionized water to the precipitate, stirring at a constant temperature until the precipitate is completely dissolved, and then separating the residual ethanol at 80-90° C. to obtain a rice bran crude polysaccharide aqueous solution, freeze-drying the rice bran crude polysaccharide aqueous solution to obtain rice bran crude polysaccharide, adding pure water to the rice bran crude polysaccharide, stirring until the rice bran crude polysaccharide is completely dissolved, and then adding α-amylase, and dripping The pH value of the solution is adjusted to 5.8-6.0 with a 0.1M citric acid-sodium citrate buffer solution, and the solution is placed in a water bath at 60°C for enzymolysis for 4-6 hours, and then 10-12 mg of saccharifying enzyme is added. After the enzymolysis is continued for 2-3 hours, the solution is heated to 100°C for constant temperature enzyme inactivation for 5 minutes, and then centrifuged at 5000 rpm for 10 minutes, the supernatant is taken, and 15% of the total volume of the supernatant TCA with a mass concentration of 60 g / L is added to the supernatant with stirring, and after stirring for 10 minutes, the solution is placed at 4°C for 30-60 minutes, the protein is filtered out, and then centrifuged at 5000 rpm for 10 minutes, the supernatant is taken, and the pH value of the system is adjusted to 7.0-7.2 with anhydrous sodium carbonate to obtain a mixed solution, and the mixed solution is dialyzed and purified with ultrapure water for 2 days. After the dialysis is completed, the solution is freeze-dried to obtain non-starch rice bran polysaccharides.

5. The highly moisturizing and antioxidant lipstick containing rice bran polysaccharides according to claim 4, characterized in that: The usage ratio of rice bran and petroleum ether in step S1 is 100:500 mL.

6. The highly moisturizing and antioxidant lipstick containing rice bran polysaccharides according to claim 4, characterized in that: The dosage ratio of the defatted rice bran powder, distilled water, deionized water, pure water, α-amylase and saccharifying enzyme in step S2 is 10.0 g: 180-200 mL: 100 mL: 80 mL: 10-12 mg: 10-12 mg.

7. The highly moisturizing and antioxidant lipstick containing rice bran polysaccharides according to claim 4, characterized in that: In step S2, the amount of α-amylase is ≥ 6000 U / mg; the amount of saccharifying enzyme is ≥ 3500 U / mg.

8. The method for preparing a highly moisturizing and antioxidant lipstick containing rice bran polysaccharides according to any one of claims 1 to 7, characterized in that: The following steps are involved: Weigh the above raw materials according to the mass ratio, add natural vegetable oil and color powder into the conical flask, ultrasonicate until the solid is completely dissolved, then add animal and plant wax, place the conical flask in a 100°C water bath to heat, wait until the material in the conical flask is completely melted, then add squalane, vitamin E, essence and rosemary essential oil into the conical flask, stir at a constant temperature for 8-10 minutes, stop stirring, obtain the material, and inject the material into the lipstick mold In the middle, shake for 15-20 minutes, put it in a refrigerator at 4-10℃ for 30 minutes, take it out, demould it, and put it into a tube. The highly moisturizing and antioxidant lipstick containing rice bran polysaccharide is obtained.

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