Traditional Chinese medicine composition for treating broiler tibial cartilage dysplasia as well as preparation method and application of traditional Chinese medicine composition

Abstract

The invention discloses a traditional Chinese medicine composition for treating broiler tibial cartilage dysplasia and a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine compositions.The traditional Chinese medicine composition is prepared from chondroitin sulfate, glucosamine, herba epimedii, eucommia ulmoides and scutellaria baicalensis according to the mass ratio of (0.5-1): (1-3): (1-2): (0.5-1): (1-2). The preparation method comprises the following steps: respectively putting all the components of the traditional Chinese medicine composition into a grinder for superfine grinding treatment, drying and screening, uniformly mixing all the components of the traditional Chinese medicine composition according to the mass ratio, and sealing and storing in a medicament bottle to obtain the traditional Chinese medicine composition. When the traditional Chinese medicine composition prepared by the invention is added into feed for feeding, the growth performance of the TD broiler chicken can be recovered to be similar to that of normal broiler chicken.

Description

Technical Field

[0001] The invention belongs to the technical field of Chinese medicine compositions, and particularly relates to a Chinese medicine composition for treating tibial chondrodysplasia in broilers, and a preparation method and application thereof. Background Art

[0002] At present, my country's poultry production has grown steadily, and the output of poultry meat and eggs has increased. In 2022, the national poultry output will be 16.14 billion, and the output of poultry meat will be 24.43 million tons. The promotion of intensive breeding technology has significantly increased my country's poultry production in just a few years, but due to the rapid weight gain of poultry and the limitation of breeding space, the incidence of tibial chondrodysplasia in broilers (hereinafter referred to as broiler TD) has increased significantly. Broiler TD is one of the common nutritional metabolic bone diseases in broilers, which can easily lead to the scrapping of broiler carcasses and cause economic losses. According to statistics, TD accounts for 30% of all broiler leg diseases, and the incidence of TD in broilers in China is about 10%. Since the discovery and report of broiler TD in academia in 1968, people have begun to pay attention to this nutritional metabolic bone disease in broilers. By exploring its causes, constantly updating and clarifying its pathogenic molecular mechanisms, we can find effective methods to prevent and treat broiler TD.

[0003] Initially, the industry believed that controlling the electrolyte intake of broiler diets could help prevent and treat TD in broilers, and proposed a dietary Na+ level suitable for the highest growth performance of broilers between 21 and 42 days of age. + and Cl - The concentration requirements are 0.15% and 0.23% respectively; although dietary addition of phytase can effectively reduce the incidence of TD in broilers, it has little protective effect on broilers with high TD incidence. Subsequent studies found that adding 35-50 μg / kg of vitamin D3 to the diet can increase the bone density of broilers to prevent TD without reducing the growth performance of broilers. Subsequently, it was discovered that not only calcium absorption, but also molecular mechanisms including angiogenesis, extracellular matrix synthesis, hypoxia, Wnt pathway, and immune-related factors are involved in the occurrence of TD in broilers. These findings also further guide people on how to optimize TD prevention and control strategies for broilers and find effective feed additives for the prevention and treatment of TD.

[0004] Based on the pathogenesis of broiler TD, the industry has found that adjusting feed formula, using Chinese herbal extracts and additives for treating broiler TD can all treat broiler TD. However, in animal husbandry production, adjusting feed formula to treat broiler TD requires frequent changes in existing feed formulas, which can easily affect the growth performance of broilers, and the condition exploration period is long, making it difficult to find a specific formula that can both treat broiler TD and optimize the growth performance of broilers; using additives to prevent and treat animal bone diseases requires considering more economic factors, and efficient additives are often more expensive due to their special preparation process, and improper use may also lead to drug residues in animal products.

[0005] Traditional natural Chinese herbal medicines have the effects of promoting bone development and improving animal production performance, and have the advantages of low cost, wide source, small toxic side effects, no drug resistance and low residue. Therefore, based on the previous research, the present invention combines natural Chinese herbal medicines, chondroitin sulfate and glucosamine to obtain a Chinese medicine composition, which is added to broiler feed for treating broiler TD and improving broiler production performance. Summary of the invention

[0006] In view of this, the present invention provides a traditional Chinese medicine composition for treating tibial chondrodysplasia in broilers, and a preparation method and application thereof.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] The invention discloses a traditional Chinese medicine composition for treating tibial chondroitin of broiler chickens. The traditional Chinese medicine composition consists of chondroitin sulfate, glucosamine, epimedium, eucommia and scutellaria; the mass ratio of the chondroitin sulfate, glucosamine, epimedium, eucommia and scutellaria is (0.5-1):(1-3):(1-2):(0.5-1):(1-2).

[0009] Preferably, the mass ratio of the chondroitin sulfate, glucosamine, epimedium, eucommia and scutellaria is 2:6:4:1:2.

[0010] The present invention also provides a method for preparing the above-mentioned Chinese medicine composition, and the specific steps are as follows: each component of the Chinese medicine composition is placed in a grinder for ultrafine grinding, and after drying and screening, the components of the Chinese medicine composition are evenly mixed according to the mass ratio, placed in a medicine bottle, sealed and stored, so as to obtain the Chinese medicine composition.

[0011] Preferably, the drying temperature is 40-60° C., and the drying time is 7-8 hours.

[0012] Preferably, the sieve used during the screening is 80-100 meshes.

[0013] The invention also discloses application of the Chinese medicine composition in feed for improving tibial chondrodysplasia in broilers.

[0014] Preferably, 7.5 grams of the Chinese medicinal composition is added to every kilogram of broiler feed.

[0015] Preferably, the contents of chondroitin sulfate, glucosamine, epimedium, eucommia and scutellaria per kilogram of broiler feed are (0.5-1) grams, (1-3) grams, (1-2) grams, (0.5-1) grams and (1-2) grams respectively.

[0016] Preferably, the contents of chondroitin sulfate, glucosamine, epimedium, eucommia bark and scutellaria baicalensis per kilogram of broiler feed are 1 gram, 3 grams, 2 grams, 0.5 grams and 1 gram respectively.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] (1) Compared with other treatment methods, the Chinese medicine composition prepared by the present invention can be added to formula feed to treat broiler TD, and its raw materials are easy to obtain, low in price, and the preparation process is simple, which reduces the steps of preparing Chinese medicine extracts.

[0019] (2) The present invention combines Epimedium with Eucommia ulmoides and Scutellaria baicalensis to increase bone density, promote cartilage differentiation and growth performance, and at the same time, chondroitin sulfate and glucosamine can relieve osteoarthritis and promote the synthesis of joint extracellular matrix. This combination of Chinese medicines enhances the therapeutic effect of broiler TD through additive effects and improves the growth performance of broilers. Figure 5 It can be seen that the Chinese medicine composition prepared by the present invention has the effect of promoting the growth and bone development of broiler chickens, and can effectively restore the tibial cartilage development of TD broiler chickens, and restore the broiler chickens to normal standing, walking, feeding and other activities, so that the TD broiler chickens can be restored to a growth performance similar to that of normal broiler chickens (such as Figure 2 ). BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a test design diagram in the effect example of the present invention;

[0021] Figure 2 This is a weight profile of broiler chickens of different ages in the effect example of the present invention;

[0022] Figure 3 This is the method for measuring TD of broiler chickens in the effect example of the present invention;

[0023] Figure 4 The general appearance of a broiler chicken and the corresponding tibia cross-section diagram in the effect example of the present invention;

[0024] Figure 5 This is a diagram showing the determination of indicators related to tibia development in broiler chickens in the effect examples of the present invention;

[0025] above Figure 2 , Figure 4 and Figure 5 All experimental data are presented as Mean±SD, “ns” indicates P>0.05, “*” indicates P<0.05, “**” indicates P<0.01, “***” indicates P<0.001, and “****” indicates P<0.0001. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] The invention discloses a traditional Chinese medicine composition for treating tibial chondrodysplasia in broilers. The traditional Chinese medicine composition consists of chondroitin sulfate, glucosamine, epimedium, eucommia bark and scutellaria baicalensis in a mass ratio of (0.5-1):(1-3):(1-2):(0.5-1):(1-2).

[0028] Among them, the optimal mass ratio of chondroitin sulfate, glucosamine, epimedium, eucommia and scutellaria is 2:6:4:1:2.

[0029] The preparation method of the above-mentioned traditional Chinese medicine composition for treating tibial chondrodysplasia of broilers comprises the following specific steps: placing each component of the traditional Chinese medicine composition into a grinder for ultrafine grinding, placing it in a drying oven at 40-60°C for drying for 7-8 hours, and then sieving it through an 80-100 mesh sieve. After that, the components of the traditional Chinese medicine composition are evenly mixed according to the above-mentioned mass ratio, and then placed in a medicine bottle for sealing and storage to obtain the traditional Chinese medicine composition.

[0030] The Chinese medicine composition prepared by the above preparation method is used in feed for improving tibial cartilage dysplasia in broilers, wherein 7.5 grams of the Chinese medicine composition is added to every kilogram of broiler feed, and the contents of chondroitin sulfate, glucosamine, epimedium, eucommia and scutellaria in every kilogram of broiler feed are (0.5-1) grams, (1-3) grams, (1-2) grams, (0.5-1) grams and (1-2) grams, respectively.

[0031] Among them, the optimal contents of chondroitin sulfate, glucosamine, epimedium, eucommia and scutellaria per kilogram of broiler feed are 1 gram, 3 grams, 2 grams, 0.5 grams and 1 gram respectively.

[0032] The technical solution of the present invention is further described below in conjunction with specific embodiments:

[0033] Example 1

[0034] The preparation method of the Chinese medicine composition for treating tibial chondrodysplasia in broiler chickens in this embodiment comprises the following specific steps:

[0035] Chondroitin sulfate, glucosamine, epimedium, eucommia and scutellaria are respectively placed in a grinder for ultrafine grinding, and then the ground components are placed in a drying oven at 56°C for drying for 8 hours, and then sieved through a 90-mesh sieve. The sieved chondroitin sulfate, glucosamine, epimedium, eucommia and scutellaria are evenly mixed in a mass ratio of 2:6:4:1:2, placed in a medicine bottle, sealed and stored, to obtain a traditional Chinese medicine composition.

[0036] Example 2

[0037] The preparation method of the Chinese medicine composition for treating tibial chondrodysplasia in broiler chickens in this embodiment comprises the following specific steps:

[0038] Chondroitin sulfate, glucosamine, epimedium, eucommia and scutellaria are respectively placed in a grinder for ultrafine grinding, and then the ground components are placed in a drying oven at 40°C for drying for 8 hours, and then sieved through an 80-mesh sieve. The sieved chondroitin sulfate, glucosamine, epimedium, eucommia and scutellaria are evenly mixed in a mass ratio of 1:3:2:1:2, placed in a medicine bottle, sealed and stored, to obtain a traditional Chinese medicine composition.

[0039] Example 3

[0040] The preparation method of the Chinese medicine composition for treating tibial chondrodysplasia in broiler chickens in this embodiment comprises the following specific steps:

[0041] Chondroitin sulfate, glucosamine, epimedium, eucommia and scutellaria are respectively placed in a grinder for ultrafine grinding, and then the ground components are placed in a drying oven at 60°C for 7 hours, and then sieved through a 100-mesh sieve. The sieved chondroitin sulfate, glucosamine, epimedium, eucommia and scutellaria are evenly mixed in a mass ratio of 1:2:2:1:2, placed in a medicine bottle, sealed and stored, to obtain a traditional Chinese medicine composition.

[0042] Example 4

[0043] The preparation method of the Chinese medicine composition for treating tibial chondrodysplasia in broiler chickens in this embodiment comprises the following specific steps:

[0044] Chondroitin sulfate, glucosamine, epimedium, eucommia and scutellaria are respectively placed in a grinder for ultrafine grinding, and then the ground components are placed in a drying oven at 60°C for drying for 8 hours, and then sieved through a 90-mesh sieve. The sieved chondroitin sulfate, glucosamine, epimedium, eucommia and scutellaria are evenly mixed in a mass ratio of 0.8:2:1.5:0.8:1.5, placed in a medicine bottle, sealed and stored, to obtain a traditional Chinese medicine composition.

[0045] Effect Example 1

[0046] The Chinese medicine combination prepared in the above Example 1 is applied in this effect example, and the application effect is as follows.

[0047] 1. Materials

[0048] (1) Experimental animals: 60 Arbor Acres broiler chickens (AA broiler chickens) (1 day old, body weight 41.53 g ± 3.87 g) were purchased from Ludong Poultry Breeding Cooperative.

[0049] (2) Preparation of Thiram Diet: Take a portion of commercial broiler feed, add 100 mg of Thiram per kg of feed, mix well, and prepare a Thiram diet containing 100 mg / kg feed weight, which is used to induce TD in broilers.

[0050] (3) Preparation of feed containing Chinese medicine composition: Add 7.5 g (1 g chondroitin, 3 g glucosamine, 2 g epimedium, 0.5 g eucommia and 1 g scutellaria) of the Chinese medicine composition prepared in Example 1 per kilogram of feed, mix well, and obtain the feed containing Chinese medicine composition in this effect column.

[0051] 2. Animal Grouping and Treatment

[0052] The above 60 healthy AA broilers of 1 day old were selected, adaptively raised until 4 days old, and randomly divided into 3 groups, 20 in each group, namely control group, TD group and Chinese medicine group. The broilers in the control group were raised with free access to food and water; the broilers in the TD group and the Chinese medicine group were fed with the above-prepared Fumei double diet from 4 days to 7 days old. During this period, the broilers in the TD group and the Chinese medicine group showed obvious TD symptoms, which were specifically manifested as the broilers unable to stand, difficulty in movement, and crouching on the ground in a split position; from 8 days to 21 days old, the diet of the broilers in the TD group was changed to the basal diet, and the diet of the broilers in the Chinese medicine group was changed to the above-mentioned diet containing the Chinese medicine composition. The experimental design diagram is as follows: Figure 1 shown.

[0053] 4. Statistical Analysis

[0054] The statistical analysis involved in all the experiments of the present invention was performed by SPSS20.0 professional statistical analysis software, and the data visualization images generated based on the statistical data were completed by GraphPad prism (9.5.0) software. Since there were 3 treatment groups in the experiment, the obtained data were analyzed by ANOVA one-way analysis of variance, and multiple comparisons were performed with the control group as a reference. In the test results, the data are presented in the form of Mean ± SD, "ns" means P> 0.05, "*" means P< 0.05, "**" means P< 0.01, "***" means P< 0.001, and "****" means P< 0.0001.

[0055] 5. Test results

[0056] 5.1 Effects of Chinese medicine composition-containing diet on growth performance of TD broilers

[0057] During the experiment, the feed intake of broilers in the control group, TD group, and Chinese medicine group was recorded, and the body weight, average daily gain (ADG), and feed conversion rate (FCR) of broilers were recorded at 1 day, 4 days, 7 days, 14 days, and 21 days of age. The calculation formulas of ADG and FCR are as follows:

[0058] ADG = (average body weight of broilers at the end of the experiment – ​​average body weight at the beginning of the experiment) / number of days of the experiment;

[0059] FCR = total feed supply (g) / total animal weight gain (g).

[0060] Since the broilers were grouped at 4 days of age and the experiment was carried out, the growth performance parameters of broilers from 4 to 21 days of age are shown in Table 1. The body weight profiles of broilers at different ages are shown in Table 1. Figure 2 shown.

[0061] Table 1

[0062] Group Feed consumption (Kg) ADG(g) FCR Control group 15.487 24.20 1.88 TD Group 14.569 23.50 1.82 Chinese Medicine Group 15.378 24.12 1.87

[0063] It can be seen from Table 1 that the feed consumption of broilers in the control group during the period of 4 to 21 days of age was 15.487Kg, ADG was 24.20g, and FCR was 1.88; the feed consumption of broilers in the TD group was 14.569Kg, ADG was 23.50g, and FCR was 1.82; the feed consumption of broilers in the Chinese medicine group was 15.378Kg, ADG was 24.12g, and FCR was 1.87; it can be seen that the growth performance parameters (feed consumption, ADG, FCR) of broilers in the TD group are lower than those in the control group and the Chinese medicine group, and the difference in growth performance parameters between the control group and the Chinese medicine group is very small, which shows that feeding the feed containing the Chinese medicine composition of the present invention can restore the growth performance of TD broilers to that of normal broilers.

[0064] Figure 2This is a body weight profile of broiler chickens at different ages in the three treatment groups (control group, TD group, and Chinese medicine group). It can be seen from the figure that: during the period of 1-7 days of age, there was no significant difference in the body weight of the three groups of broiler chickens (P>0.05), but at this stage, the broiler chickens in the TD group and the Chinese medicine group showed obvious TD symptoms; with the increase of age, the body weight of broiler chickens in the control group was significantly higher than that of broiler chickens in the TD group and the Chinese medicine group at 14 days of age (P<0.0001); at 21 days of age, the body weight of broiler chickens in the TD group was still significantly lower than that of broiler chickens in the control group (P<0.05), while there was no significant difference in the body weight of broiler chickens in the Chinese medicine group compared with that in the control group (P>0.05), which shows that: feed containing the Chinese medicine composition can promote the growth of TD broiler chickens and restore the body weight of TD broiler chickens to the level of normal broiler chickens.

[0065] 5.2 Effects of Chinese herbal medicine combination on TD in broiler chickens

[0066] When the broilers were grown to 21 days old, they were killed by cervical dislocation, and the tibia of the broilers was taken out to measure the tibia weight, tibia length, tibia width, and cartilage surface width of the broilers. The measurement method is as follows: Figure 3 shown.

[0067] Figure 4 Figure 1 shows the general appearance of broilers and their corresponding tibia cross-sections. From Figure (a), we can see that: the broilers in the control group ate and drank normally, were lively and active, and had full wings; the broilers in the TD group were reluctant to eat and liked to lie down at the beginning. Due to long-term crouching, their bodies were easily contaminated with feces, which resulted in the inability to grow their wings normally. As they grew older, the symptoms of movement disorders in the TD broilers were alleviated, and they could eat and move around, but they still had symptoms such as lying down and lameness; the broilers in the Chinese medicine group also showed reluctance to eat and lying down in the early stage, but after several days of Chinese medicine treatment, they could stand, walk, eat and move normally, with a good mental state, lively and active, normal body shape, and acceptable wing growth. In order to further compare the development of the tibial growth plate of broiler chickens in the control group, TD group and Chinese medicine group, the tibiae of broiler chickens in different groups were randomly peeled, and a section was made at the proximal end of the epiphysis using a scalpel as shown in Figure (b). The results showed that the growth plate area of ​​broiler chickens in the control group was normal, an irregular, non-vascularized, non-calcified white cartilage plug was found in the growth plate of broiler chickens in the TD group, and the area of ​​the white cartilage plug in the tibial growth plate of broiler chickens in the Chinese medicine group gradually shrank or tended to disappear, and there were obvious signs of vascular invasion in the growth plate (the area marked by the dotted line in Figure b is the tibial cartilage surface).

[0068] In order to further analyze the treatment of TD in broiler chickens by the Chinese medicine composition, the indicators related to tibia development in broiler chickens were measured, such as Figure 5As shown. As can be seen from Figures (a) and (b), compared with the control group, there was no significant difference in the tibial mass and tibial index of broiler chickens in the TD group (P>0.05), while the tibial mass and tibial index of broiler chickens in the Chinese medicine group were significantly higher than those in the control group and the TD group (P<0.05); As can be seen from Figures (c) and (d), compared with the control group, there was no significant difference in the tibial length and width of broiler chickens in the TD group (P>0.05), while the tibial mass and tibial index of broiler chickens in the Chinese medicine group were significantly higher than those in the control group and the TD group (P<0.05), which shows that the Chinese medicine composition prepared by the present invention has the effect of promoting the growth and bone development of broiler chickens. Since one of the main factors for the occurrence of TD in broiler chickens is that the bone development speed of broiler chickens cannot keep up with their own excessively fast growth rate, resulting in the inability of bones to bear the excessively high weight of broiler chickens, further inducing the occurrence of TD in broiler chickens, the application of the Chinese medicine composition prepared by the present invention in feed can improve the growth performance of TD broiler chickens and enhance their bone development. At the same time, the width of the cartilage surface is considered to be one of the indicators that is highly correlated with the occurrence of TD in broiler chickens. The increase in the width of the cartilage surface of broiler chickens often indicates that the process of endochondral ossification of the tibia of broiler chickens is blocked, resulting in the failure of hypertrophy and differentiation of the tibial cartilage. As can be seen from Figure (e), compared with the control group, the width of the cartilage surface of the broiler chickens in the TD group increased significantly (P < 0.001), while the width of the cartilage surface of the TD broiler chickens in the Chinese medicine group fed with Chinese medicine feed was not significantly different from that in the control group and the TD group (P > 0.05). This shows that the application of the Chinese medicine composition prepared by the present invention in the feed can effectively restore the development of the tibial cartilage of TD broiler chickens, and enable the broiler chickens to resume normal standing, walking, feeding and other activities.

[0069] The above is a detailed introduction to a Chinese medicine composition for treating tibial chondrodysplasia in broilers disclosed in the present invention, as well as its preparation method and application. Specific examples are used herein to illustrate the principles and implementation methods of the present invention, and the description of the above embodiments is only used to help understand the method of the present invention and its core idea. It should be pointed out that for ordinary technicians in this technical field, without departing from the principle of the present invention, the present invention can also be improved and modified in a number of ways, and these improvements and modifications also fall within the scope of protection of the claims of the present invention.

Claims

1. A Chinese medicine composition for treating tibial chondrodysplasia in broiler chickens, It is characterized in that The Chinese medicine composition is composed of chondroitin sulfate, glucosamine, epimedium, eucommia and scutellaria; the mass ratio of the chondroitin sulfate, glucosamine, epimedium, eucommia and scutellaria is (0.5-1): (1-3): (1～2)：(0.5～1)：(1～2)。 2. A Chinese medicine composition for treating tibial chondrodysplasia in broilers according to claim 1, It is characterized in that The mass ratio of the chondroitin sulfate, glucosamine, epimedium, eucommia bark and scutellaria baicalensis is 2:6:4:1:

2.

3. A method for preparing the Chinese medicine composition according to any one of claims 1 to 2, It is characterized in that The specific steps are as follows: put the components of the traditional Chinese medicine composition into a grinder for ultrafine grinding, dry and screen, mix the components of the traditional Chinese medicine composition evenly according to the mass ratio, put them in a medicine bottle, seal and store them, and then obtain the traditional Chinese medicine composition.

4. The method for preparing the Chinese medicine composition according to claim 3, It is characterized in that The drying temperature is 40-60° C., and the drying time is 7-8 hours.

5. The method for preparing the Chinese medicine composition according to claim 3, It is characterized in that The mesh size of the sieve during the screening is 80 to 100 meshes.

6. Use of the Chinese medicine composition according to any one of claims 1 to 2 in feed for improving tibial chondrodysplasia in broilers.

7. The use according to claim 6, It is characterized in that 7.5 grams of the Chinese medicinal composition needs to be added to each kilogram of broiler feed.

8. The use according to claim 7, It is characterized in that The contents of chondroitin sulfate, glucosamine, epimedium, eucommia bark and scutellaria baicalensis in each kilogram of broiler feed are (0.5-1) gram, (1-3) gram, (1-2) gram, (0.5-1) gram and (1-2) gram respectively.

9. The use according to claim 8, It is characterized in that The contents of chondroitin sulfate, glucosamine, epimedium, eucommia bark and scutellaria baicalensis in each kilogram of broiler feed are 1 gram, 3 grams, 2 grams, 0.5 grams and 1 gram respectively.

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