Microfluidic operation equipment

Abstract

The invention discloses a microfluidic operation device which comprises a core module used for bearing and controlling liquid flow, a temperature adjusting module used for adjusting the temperature of the core module, a heat dissipation module used for reducing the temperature of the temperature adjusting module and a control module. The core module, the temperature adjusting module and the heat dissipation module are all in communication connection with the control module; in conclusion, the temperature of the core module is adjusted by using the temperature adjusting module, so that the core module can meet the temperature requirement of a biological experiment; the accuracy and repeatability of experimental results are ensured; in order to avoid the situation that the heat dissipation efficiency is reduced due to the fact that the temperature of the temperature adjusting module is too high, the invention further provides a heat dissipation module which is used for cooling the temperature adjusting module and transmitting the temperature of the temperature adjusting module to the heat dissipation module so that the temperature adjusting module can be kept in a high-efficiency state all the time.

Description

Technical Field

[0001] The present invention relates to the technical field of microfluidic operation equipment, and in particular to a microfluidic operation equipment. Background Art

[0002] In the prior art, microfluidics refers to the technology of accurately manipulating and processing fluids at micrometers or smaller scales. Microfluidics utilizes physical and chemical effects in tiny channels so that trace amounts of liquids can be manipulated with extremely high precision, such as flowing, mixing, reacting, separating and detecting. Compared with traditional fluid operations, microfluidics can handle extremely small volumes of liquids (usually nanoliters to picoliters), and has higher efficiency, faster response speed and lower reagent consumption.

[0003] In many microfluidic chips, electrowetting is used to move or manipulate droplets. Electrowetting changes the contact angle of droplets on the surface by frequently switching the electric field, thereby moving droplets. Many chemical and biological reactions are very sensitive to temperature. When performing these reactions in a microfluidic chip, slight changes in temperature may significantly affect the reaction rate and results, so it is necessary to control the temperature of the microfluidic chip to meet the temperature requirements of biological experiments. Summary of the invention

[0004] The purpose of the present invention is to disclose a microfluidic operating device, which solves the problem that the temperature of the microfluidic chip cannot be controlled in the existing microfluidic operating device.

[0005] In order to achieve the above object, the present invention adopts the following technical scheme:

[0006] A microfluidic operation device comprises: a core module for carrying and controlling the flow of liquid, a temperature regulating module for adjusting the temperature of the core module, a heat dissipation module for reducing the temperature of the temperature regulating module, and a control module; the core module, the temperature regulating module and the heat dissipation module are all communicatively connected with the control module.

[0007] In one embodiment, the temperature regulating module includes at least one semiconductor chip; one side of the semiconductor chip is fixedly connected to the bottom of the core module for increasing or decreasing the temperature of the core module; and the semiconductor chip is communicatively connected to the control module.

[0008] In one embodiment, the heat dissipation module includes: a water cooling box assembly, a water pipe and a water cooling heat exchange plate; the water cooling heat exchange plate is fixedly connected to the other side of the semiconductor chip for adjusting the temperature of the other side of the semiconductor chip; a water channel is provided inside the water cooling heat exchange plate; the water channel is connected to the water cooling box assembly through the water pipe for realizing the circulation of cooling water; the water cooling box assembly is communicatively connected to the control module.

[0009] In one embodiment, the temperature regulating module includes four semiconductor chips; one side of the four semiconductor chips is fixedly connected to the bottom of the core module; and the other side of the four semiconductor chips is fixedly connected to the water-cooled heat exchange plate.

[0010] In one embodiment, the water cooling box assembly includes: a water pump for promoting the flow of cooling water, a heat dissipation metal row for increasing the heat exchange area between the cooling water and the environment, and a fan for promoting air flow to accelerate the heat exchange of the heat dissipation metal row; the water-cooled heat exchange plate is connected to the heat dissipation metal row through the water pipe and the water pump in turn; the water pump and the fan are both communicatively connected to the control module.

[0011] In one embodiment, it also includes: a base; a first linear module for driving the core module to move linearly is fixedly arranged on the base; the core module is fixedly connected to the driving end of the first linear module; the first linear module is communicatively connected to the control module.

[0012] In one embodiment, it also includes: a camera for monitoring and observing the flow of liquid and a two-axis drive assembly for driving the camera to move; a portal frame is fixedly arranged on the base; the two-axis drive assembly is fixedly connected to the portal frame; the camera is fixedly connected to the driving end of the two-axis drive assembly; the camera and the two-axis drive assembly are both communicatively connected to the control module.

[0013] In one embodiment, the two-axis drive assembly includes: a second linear module for driving the camera to move horizontally linearly and a third linear module for driving the camera to move vertically linearly; the second linear module is fixedly connected to the portal frame; the third linear module is fixedly connected to the driving end of the second linear module; the second linear module and the third linear module are both communicatively connected to the control module; the camera is fixedly connected to the driving end of the third linear module; the first linear module, the second linear module and the third linear module are all perpendicular to each other.

[0014] In one embodiment, it also includes: a magnetic control module for performing magnetic bead-based nucleic acid extraction in biological experiments; the magnetic control module is fixedly connected to the driving end of the first linear module; and the magnetic control module is communicatively connected to the communication module.

[0015] In one embodiment, the magnetic control module includes: a magnetic component for adsorbing the nucleic acid-magnetic bead complex on the microfluidic chip to control the flow direction of the nucleic acid-magnetic bead complex, a first driving component for controlling the linear movement of the magnetic component along a first direction, a second driving component for controlling the linear movement of the magnetic component along a second direction, and a third driving component for controlling the linear movement of the magnetic component along a third direction; the magnetic component is transmission-connected to the driving end of the first driving component; the first driving component is transmission-connected to the driving end of the second driving component; the second driving component is transmission-connected to the driving end of the third driving component; the third driving component is transmission-connected to the driving end of the first linear module; the first driving component, the second driving component and the third driving component are all communicatively connected to the control module.

[0016] In one embodiment, the magnetic attraction component includes: a magnet and a connecting member; the magnet is fixedly connected to one end of the connecting member; and the other end of the connecting member is transmission-connected to the driving end of the first driving component.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] The present invention provides a microfluidic operation device, comprising: a core module for carrying and controlling the flow of liquid, a temperature regulating module for adjusting the temperature of the core module, a heat dissipation module for reducing the temperature of the temperature regulating module, and a control module; the core module, the temperature regulating module and the heat dissipation module are all communicatively connected with the control module; in summary, the present invention uses the temperature regulating module to dissipate heat for the core module, and uses the control module to control the temperature of the temperature regulating module, thereby preventing experimental errors caused by temperature and ensuring the accuracy and repeatability of experimental results; in order to avoid the temperature of the temperature regulating module being too high, resulting in reduced heat dissipation efficiency, the present application further provides a heat dissipation module for cooling the temperature regulating module, so that the temperature of the temperature regulating module can be transmitted to the heat dissipation module, so that the temperature regulating module can always be kept in a high-efficiency state. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the accompanying drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings in the following description are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative labor.

[0020] Figure 1 It is a three-dimensional schematic diagram of a microfluidic operation device of the present invention;

[0021] Figure 2 It is a three-dimensional schematic diagram of the structure of the magnetic control module;

[0022] In the figure, 1. core module; 2. temperature regulating module; 21. semiconductor chip; 3. heat dissipation module; 31. water cooling box; 32. water cooling heat exchange plate; 4. base; 41. first linear module; 5. door frame; 51. second linear module; 52. third linear module; 53. camera; 6. magnetic control module; 611. magnet; 612. horizontal connecting rod; 613. vertical connecting rod; 615. fixed block; 621. first structural member; 622. first guide rail; 623. first slider; 624. first screw motor; 625. first screw nut; 631. second structural member; 632. second guide rail; 633. second slider; 634. second screw motor; 635. third structural member; 641. bottom plate; 642. third guide rail; 643. third slider; 644. third screw motor. DETAILED DESCRIPTION

[0023] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal connection of two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to the specific circumstances. The terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features.

[0024] In the present invention, unless otherwise clearly specified and limited, the first feature being "above" or "below" the second feature may include the first and second features being in direct contact, or may include the first and second features being in contact not directly but through another feature between them. Moreover, the first feature being "above", "above" and "above" the second feature includes the first feature being directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. The first feature being "below", "below" and "below" the second feature includes the first feature being directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature. The terms "vertical", "horizontal", "left", "right", "above", "below" and similar expressions are for illustrative purposes only, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0025] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0026] Embodiment 1

[0027] like Figure 1 A microfluidic operating device is shown, comprising: a core module 1 for carrying and controlling the flow of liquid, a temperature regulating module for reducing the temperature of the core module 1, a heat dissipation module for adjusting the temperature of the temperature regulating module, and a control module; the core module 1, the temperature regulating module and the heat dissipation module are all communicatively connected to the control module.

[0028] Specifically, in the present application, the temperature of the core module 1 is adjusted by the temperature regulating module, and the temperature of the temperature regulating module is controlled by the control module, which can prevent the experimental error caused by temperature. When the biological nucleic acid reaction is carried out in the microfluidic chip, the slight change of temperature may significantly affect the reaction rate and the result. The reaction temperature can be controlled within a specific range by cooling, so as to ensure the accuracy and repeatability of the experimental results. Further, in order not to affect the volume of the core module 1, it is necessary to reduce the volume of the temperature regulating module as much as possible, which will affect the temperature control ability of the temperature regulating module and reduce the temperature regulation efficiency. In order to avoid the temperature of the temperature regulating module being too high, resulting in a decrease in heat dissipation efficiency, the present application further provides a heat dissipation module for cooling the temperature regulating module so that the temperature of the temperature regulating module can be transferred to the heat dissipation module, so that the efficiency of the temperature regulating module can be kept at a relatively high state at all times. By connecting the core module 1, the temperature regulating module, the heat dissipation module and the control module for communication, efficient integration and automatic management of temperature control are achieved. The modular design of the entire device improves the maintainability and scalability of the system.

[0029] In one embodiment, the temperature regulating module includes at least one semiconductor chip 21; one side of the semiconductor chip 21 is fixedly connected to the bottom of the core module 1 for increasing or decreasing the temperature of the core module 1; the semiconductor chip 21 is communicatively connected to the control module.

[0030] The core module 1 is directly cooled using the semiconductor chip 21, achieving fast and accurate temperature control. The use of the semiconductor chip 21 makes the heat dissipation module small in size and high in efficiency, which is suitable for the needs of precise temperature management in microfluidic devices. The cooling principle of the semiconductor chip 21 is based on the Peltier effect, which is a thermoelectric effect. When an electric current passes through a galvanic junction composed of two different materials, heat is absorbed from one junction and released at the other junction. If a direct current is applied to a galvanic couple connecting two different semiconductor materials (such as N-type and P-type semiconductors), the current passing through the junction will cause one end to absorb heat (cooling) and the other end to release heat. When a direct current passes through these semiconductor materials, electrons move from a low energy state (near the cold end) to a high energy state (near the hot end) in the N-type material, and holes move from a high energy state (near the hot end) to a low energy state (near the cold end) in the P-type material. During the flow of current, the cold end (the end that contacts the cooling object) absorbs heat because energy is required when electrons move from the cold end to the hot end, which causes the temperature of the cold end to drop. On the contrary, the hot end (the end exposed to the external environment) will release the heat carried by these electrons, causing the temperature of the hot end to rise. By adjusting the direction of the current in the semiconductor chip, the cold end and the hot end of the semiconductor can be controlled to switch back and forth.

[0031] In one embodiment, the heat dissipation module includes: a water cooling box 31 component, a water pipe (not shown in the figure) and a water-cooled heat exchange plate 32; the water-cooled heat exchange plate 32 is fixedly connected to the hot end of the semiconductor chip 21, and is used to reduce the temperature of the other side of the semiconductor chip 21; a water channel is opened inside the water-cooled heat exchange plate 32; the water channel is connected to the water cooling box 31 component through the water pipe, and is used to realize the circulation of cooling water; the water cooling box 31 component is communicatively connected to the control module.

[0032] In order to ensure the effective operation of the semiconductor wafer 21, a water-cooled heat exchange plate 32 capable of efficiently transferring heat is provided at the hot end of the semiconductor wafer 21 in the present application. The water-cooled heat exchange plate 32 is in close contact with the hot end of the semiconductor wafer 21, and absorbs the heat of the hot end of the semiconductor wafer 21 through heat exchange. In addition, by providing a water channel inside the heat exchange plate, the cooling water flowing through the heat exchange plate can take away the heat of the hot end of the semiconductor wafer 21, thereby achieving rapid cooling of the semiconductor wafer 21 and maintaining the semiconductor wafer 21 at a higher working efficiency.

[0033] Similarly, when the semiconductor chip 21 heats the core module, the water-cooled heat exchange plate 32 can also provide heat for the semiconductor chip 21 to prevent the cold end temperature of the semiconductor chip from being too low.

[0034] In one embodiment, the temperature regulating module includes four semiconductor chips 21 ; the cold ends of the four semiconductor chips 21 are fixedly connected to the bottom of the core module 1 ; and the hot ends of the four semiconductor chips 21 are fixedly connected to the water-cooled heat exchange plate 32 .

[0035] In order to improve the heat dissipation capacity of the core module 1 and the area of ​​the core module 1, a total of four semiconductor chips 21 are set at the bottom of the core module 1 in this application. The combined use of the four semiconductor chips 21 can evenly and stably adjust the temperature of the core module 1, avoiding a single semiconductor chip being unable to cover the temperature control requirements of the entire core module 1, thereby improving the temperature control effect of the core module and the reliability of the device. The control module can also adjust the working state of each semiconductor chip 21 according to the actual area of ​​liquid flow on the core module 1 to adapt to different working states.

[0036] In the present application, the control module can control the cold end and the hot end of the semiconductor chip by controlling the direction of the current. In the case of forward current, the side of the semiconductor chip connected to the core module is cooled, and the side connected to the water-cooled heat exchange plate 32 is heated, and the water-cooled heat sink is used to absorb the heat emitted from the hot end. In the case of reverse current, the side of the semiconductor chip connected to the core module is heated, and the side connected to the water-cooled heat exchange plate 32 is cooled, and the water-cooled heat sink is used to release heat to prevent the cold end temperature of the semiconductor chip from being too low.

[0037] In one embodiment, the water cooling box 31 assembly includes: a water pump for promoting the flow of cooling water, a heat dissipation metal row for increasing the heat exchange area between the cooling water and the environment, and a fan for promoting air flow to accelerate the heat exchange of the heat dissipation metal row; the water-cooled heat exchange plate 32 is connected to the heat dissipation metal row through the water pipe and the water pump in turn; the water pump and the fan are both communicatively connected to the control module.

[0038] Specifically, the water cooling box 31 component design, which integrates a water pump, a heat dissipation metal row and a fan, can efficiently discharge heat from the hot surface of the refrigeration plate to the environment, ensure the circulation and heat dissipation effect of the cooling water, and further optimize the temperature management of the system. In addition, the water pump and fan in the water cooling box 31 component can also communicate with the control module. The control module can dynamically adjust the working status of the water pump and fan according to the temperature to increase the service life of the water pump and fan.

[0039] In one embodiment, it also includes: a base 4; a first linear module 41 for driving the core module 1 to move linearly is fixedly arranged on the base 4; the core module 1 is fixedly connected to the driving end of the first linear module 41; the first linear module 41 is communicatively connected to the control module.

[0040] In one embodiment, it also includes: a camera 53 for monitoring and observing the flow of liquid and a two-axis drive assembly for driving the camera 53 to move; a portal frame 5 is fixedly arranged on the base 4; the two-axis drive assembly is fixedly connected to the portal frame 5; the camera 53 is fixedly connected to the driving end of the two-axis drive assembly; the camera 53 and the two-axis drive assembly are both communicatively connected to the control module.

[0041] Specifically, the first linear module 41 is used to control the position of the core module 1 so that the core module 1 can be aligned with the camera 53 , so that the camera 53 can monitor and observe the flow state of the liquid on the core module 1 .

[0042] In one embodiment, the two-axis drive assembly includes: a second linear module 51 for driving the camera 53 to move horizontally linearly and a third linear module 52 for driving the camera 53 to move vertically linearly; the second linear module 51 is fixedly connected to the portal frame 5; the third linear module 52 is fixedly connected to the driving end of the second linear module 51; the second linear module 51 and the third linear module 52 are both communicatively connected to the control module; the camera 53 is fixedly connected to the driving end of the third linear module 52; the first linear module 41, the second linear module 51 and the third linear module 52 are all perpendicular to each other.

[0043] Specifically, the second linear module 51 in the two-axis drive assembly is used to control the horizontal movement of the camera 53, and the third linear module 52 is used to control the up and down movement of the camera 53. The first linear module 41 is then used to control the up and down movement of the core module 1. The camera 53 can be accurately aligned with the liquid flowing on the core module 1, so that the camera 53 can accurately capture the flow of the liquid.

[0044] In the present application, the core module 1 specifically adopts TFT and electrowetting technology to drive liquid flow. In the microfluidic chip, TFT is responsible for applying and adjusting voltages, which act on electrodes covered with dielectric layers. When voltage is applied to the electrodes, the contact angle of the liquid on the surface of the medium will change, thereby affecting the shape and movement of the droplets. For example, TFT can turn on or off electrodes in different areas so that droplets move, separate or merge on a predetermined path. In addition, by accurately controlling the on-state of TFT, local electric fields can be generated in different areas of the microfluidic chip. These electric fields change the surface tension of the droplets, causing them to flow along a specific path. In addition, the present application also combines display flat panel technology, which is used to provide an interactive interface to display the status or data during the experiment, thereby improving the convenience of the device operation in the present embodiment.

[0045] In one embodiment, the control module is an MCU or a single-chip microcomputer. The MCU module or the single-chip microcomputer can calculate the specific positions of the three linear modules according to a pre-set program and input specific parameters to align the camera 53 with the flow position of the liquid. In addition, it can also control the temperature of the temperature adjustment module and the heat dissipation module according to predetermined needs to avoid excessive temperature changes affecting the experimental results.

[0046] In summary, the present application proposes a microfluidic operation device, which can accurately adjust the temperature of the core module 1 under the control of the control module by setting a plurality of semiconductor chips 21, and can adjust the working efficiency of the semiconductor chip 21 by using water cooling to dissipate the heat at the hot end of the semiconductor chip 21, so as to avoid overheating and damage of the semiconductor chip 21. The core module 1 adopts a combination of TFT and electrowetting technology, which can accurately control the movement, separation and merging of droplets. By adjusting the voltage on the electrode, the flow path and state of the liquid in the microfluidic chip can be flexibly controlled to meet different experimental needs. Through the integrated display flat panel technology, the device provides an intuitive interactive interface that can display the experimental status and data in real time, which not only improves the convenience of equipment operation, but also improves the efficiency of experimental monitoring, and the user can operate and control more intuitively.

[0047] Embodiment 2

[0048] As a further improvement to the present invention, Figure 2As shown, a microfluidic operation device of embodiment 2 also includes: a magnetic control module 6 for performing magnetic bead-based nucleic acid extraction in biological experiments; the magnetic control module 6 is fixedly connected to the driving end of the first linear module; the magnetic control module 6 is communicatively connected to the communication module.

[0049] Specifically, the operating device of the present application further adds a magnetic control method based on the electrowetting control, which can improve the application range of the microfluidic operating device. The magnetic control module 6 is arranged on the first linear module, so that the magnetic control module 6 and the core module can maintain synchronous displacement, reducing the calculation difficulty of the device.

[0050] In one embodiment, the magnetic control module 6 includes: a magnetic component for adsorbing the nucleic acid-magnetic bead complex on the microfluidic chip to control the flow direction of the nucleic acid-magnetic bead complex, a first driving component for controlling the linear movement of the magnetic component along a first direction, a second driving component for controlling the linear movement of the magnetic component along a second direction, and a third driving component for controlling the linear movement of the magnetic component along a third direction; the magnetic component is transmission-connected to the driving end of the first driving component; the first driving component is transmission-connected to the driving end of the second driving component; the second driving component is transmission-connected to the driving end of the third driving component; the third driving component is transmission-connected to the driving end of the first linear module; the first driving component, the second driving component and the third driving component are all communicatively connected to the control module.

[0051] Specifically, in this embodiment, by setting three mutually perpendicular drive components, the magnetic suction component can be driven to move in the three spaces of XYZ respectively, and the control component is used to control the moving distances of the first drive component, the second drive component and the third drive component respectively, so that the moving position of the magnetic suction component can be efficiently and accurately controlled, and the moving path of the magnetic suction component can be effectively repeated. In a large number of repeated microfluidic experiments, the accuracy of droplet movement can be effectively improved. Among them, the first drive component is used to control the up and down movement of the magnetic suction component in the vertical direction, and the strength of the magnetic suction can be controlled by controlling the distance between the magnetic suction component and the microfluidic chip. The second drive component and the third drive component are used to control the position of the magnetic suction component on the horizontal plane. The two components cooperate with each other to accurately adjust the position of the magnetic suction component, thereby controlling the flow direction of the liquid in the microfluidic chip.

[0052] In one embodiment, the magnetic attraction component includes: a magnet 611 and a connecting member; the magnet 611 is fixedly connected to one end of the connecting member; and the other end of the connecting member is drivingly connected to the driving end of the first driving component.

[0053] Specifically, in the present application, the magnet 611 is used to adsorb the nucleic acid-magnetic bead complex in the microfluidic chip, so that the nucleic acid-magnetic bead complex can flow along the pipeline in the microfluidic chip under the control of the magnet 611. Since the driving component is large in size, if it is close to the microfluidic chip, it will occupy a large amount of space and affect the observation of the microfluidic chip. Therefore, a longer connecting piece is used to maintain a certain distance between the magnet 611 and the driving component, so as to avoid the driving component blocking the observation line of sight.

[0054] In one embodiment, the first drive component includes a first structural member 621, a first guide rail 622, a first slider 623 and a first screw motor 624; the first screw motor 624 is communicatively connected to the control component; the first structural member 621 is fixedly connected to the driving end of the second drive component; the first guide rail 622 and the first screw motor 624 are both fixedly connected to the first structural member 621; the first slider 623 is slidably connected to the first guide rail 622, the first slider 623 is fixedly connected to the first screw nut 625 of the first screw motor 624, and the other end of the connecting member is fixedly connected to the first screw nut 625.

[0055] In the present application, the first screw nut 625 on the first screw motor 624 is meshed with the screw of the first screw motor 624; the first screw nut 625 is fixedly connected to the first slider 623, which limits the rotational freedom of the first screw nut 625. Therefore, when the first screw motor 624 drives the screw to rotate, the first screw nut 625 can convert the rotation of the screw into the linear motion of the first screw nut 625 and the first slider 623, and then can drive the connector and magnet 611 fixedly connected to the first screw nut 625 to perform linear motion. Since the first screw motor 624 is a servo motor or a stepper motor, the angle of rotation of the motor can be controlled by the pre-programmed program of the control component, and the amplitude of the motor rotation can be accurately calculated according to the movement distance of the magnet 611. The connection between the connector and the first screw nut 625 is a screw connection, which can be disassembled and maintained as needed, thereby increasing the service life of the equipment.

[0056] In one embodiment, the second drive component includes: a second structural member 631, a second guide rail 632, a second slider 633 and a second screw motor 634; the second screw motor 634 is communicatively connected to the control component; the second structural member 631 is fixedly connected to the driving end of the third drive component; the second guide rail 632 and the second screw motor 634 are both fixedly connected to the second structural member 631; the second slider 633 is slidably connected to the second guide rail 632, and the second slider 633 is fixedly connected to the second screw nut (not shown in the figure) of the second screw motor 634; the first structural member 621 is fixedly connected to the second screw nut.

[0057] In the present application, the second screw nut on the second screw motor 634 is equal to the screw of the second screw motor 634, and the second screw nut is fixedly connected to the second slider 633, which limits the rotational freedom of the second screw nut. Therefore, when the second screw motor 634 drives the screw to rotate, the second screw nut can convert the rotation of the screw into the linear motion of the second screw nut and the second slider 633, and then drive the first drive component and the magnetic suction component fixedly connected to the second screw nut to perform linear motion. And the direction of the linear motion is parallel to the direction of the second guide rail 632.

[0058] In one embodiment, the second driving assembly further includes: a third structural member 635; the third structural member 635 is respectively fixedly connected to the second screw nut and the second slider 633; ​​the first structural member 621 is fixedly connected to the third structural member 635; the third structural member 635 and the first structural member 621 are perpendicular to each other.

[0059] In the present application, the third structural member 635 is used to adjust the angle of the first driving assembly to avoid mutual interference between the first driving assembly and the second driving assembly. In the present application, the third structural member 635 and the first structural member 621 are perpendicular to each other.

[0060] In one embodiment, the third drive component includes: a base plate 641, a third guide rail 642, a third slider 643 and a third screw motor 644; the third screw motor 644 is communicatively connected to the control component; the third guide rail 642 and the third screw motor 644 are both fixedly connected to the base plate 641; the third slider 643 is slidably connected to the third guide rail 642, and the third slider 643 is fixedly connected to the third screw nut (not shown in the figure) of the third screw motor 644; the second structural member 631 is fixedly connected to the third screw nut.

[0061] In the present application, the third screw nut on the third screw motor 644 is equal to the screw meshing connection of the third screw motor 644, and the third screw nut is fixedly connected to the third slider 643, which limits the rotational freedom of the third screw nut. Therefore, when the third screw motor 644 drives the screw to rotate, the third screw nut can convert the rotation of the screw into the linear motion of the third screw nut and the third slider 643, and then can drive the second drive assembly, the first drive assembly and the magnetic suction assembly fixedly connected to the third screw nut to perform linear motion. And the direction of the linear motion is parallel to the direction of the third guide rail 642. The second drive assembly and the third drive assembly cooperate with each other to control the magnet 611 to move to any position on the horizontal plane. When the control assembly is pre-calculated, the control efficiency of the magnet 611 in the repeated action can be effectively improved. Compared with manually controlling the movement of the magnet 611, the repetition efficiency is fast and the control accuracy is high.

[0062] In one embodiment, the connecting member includes: a horizontal connecting rod 612, a vertical connecting rod 613, a spring and a fixing block 615; the magnet 611 is fixedly connected to one end of the fixing block 615; the other end of the fixing block 615 is slidably connected to the bottom of the vertical connecting rod 613; the top of the vertical connecting rod 613 is fixedly connected to one end of the horizontal connecting rod 612; the other end of the horizontal connecting rod 612 is transmission-connected to the driving end of the first driving assembly; the spring is contained inside the vertical connecting rod 613, one end of the spring abuts against the inner wall of the vertical connecting rod 613; the other end of the spring abuts against the fixing block 615. In this embodiment, the spring can provide a buffer stroke when the magnet 611 is pressed down close to the core module to prevent the magnet from crushing the core module.

[0063] The present invention is not limited to the above-mentioned embodiments. If various changes or modifications to the present invention do not depart from the spirit and scope of the present invention, and if these changes and modifications fall within the scope of the claims and equivalent technologies of the present invention, the present invention is also intended to include these changes and modifications.

Claims

1. A microfluidic operation device, characterized in that: include: A core module for carrying and controlling the flow of liquid, a temperature regulating module for adjusting the temperature of the core module, a heat dissipation module for reducing the temperature of the temperature regulating module, and a control module; The core module, the temperature adjustment module and the heat dissipation module are all connected to the control module for communication.

2. A microfluidic operation device according to claim 1, characterized in that: The temperature regulating module includes at least one semiconductor chip; one side of the semiconductor chip is fixedly connected to the bottom of the core module, and is used to increase or decrease the temperature of the core module; The semiconductor chip is communicatively connected with the control module.

3. A microfluidic operation device according to claim 2, characterized in that: The heat dissipation module includes: a water cooling box assembly, a water pipe and a water cooling heat exchange plate; The water-cooled heat exchange plate is fixedly connected to the other side of the semiconductor chip, and is used to adjust the temperature of the other side of the semiconductor chip; a water channel is provided inside the water-cooled heat exchange plate; the water channel is connected to the water cooling box assembly through the water pipe, and is used to realize the circulation of cooling water; The water cooling box assembly is communicatively connected with the control module.

4. A microfluidic operation device according to claim 3, characterized in that: The temperature regulating module comprises four semiconductor chips; one side of each of the four semiconductor chips is fixedly connected to the bottom of the core module; The other surfaces of the four semiconductor sheets are fixedly connected to the water-cooled heat exchange plate.

5. A microfluidic operation device according to claim 4, characterized in that: The water cooling box assembly includes: a water pump for promoting the flow of cooling water, a heat dissipation metal row for increasing the heat exchange area between the cooling water and the environment, and a fan for promoting air flow to accelerate the heat exchange of the heat dissipation metal row; The water-cooled heat exchange plate is connected to the heat dissipation metal row through the water pipe and the water pump in sequence; The water pump and the fan are both in communication connection with the control module.

6. The microfluidic operation device according to claim 1, characterized in that: Also includes: Base; A first linear module for driving the core module to move linearly is fixedly arranged on the base; The core module is fixedly connected to the driving end of the first linear module; The first linear module is communicatively connected to the control module.

7. A microfluidic operation device according to claim 6, characterized in that: Also includes: A camera for monitoring and observing the flow of liquid and a two-axis driving assembly for driving the camera to move; A door frame is fixedly arranged on the base; the two-axis driving assembly is fixedly connected to the door frame; the camera is fixedly connected to the driving end of the two-axis driving assembly; The camera and the two-axis driving components are both connected to the control module for communication.

8. The microfluidic operation device according to claim 7, characterized in that: The two-axis drive assembly comprises: A second linear module for driving the camera to move horizontally linearly and a third linear module for driving the camera to move vertically linearly; The second linear module is fixedly connected to the portal frame; The third linear module is fixedly connected to the driving end of the second linear module; The camera is fixedly connected to the driving end of the third linear module; The second linear module and the third linear module are both communicatively connected to the control module; The first linear module, the second linear module and the third linear module are all perpendicular to each other.

9. The microfluidic operation device according to claim 1, characterized in that: Also includes: A magnetic suction control module for performing magnetic bead-based nucleic acid extraction in biological experiments; The magnetic control module is fixedly connected to the driving end of the first linear module; The magnetic attraction control module is communicatively connected with the communication module.

10. The microfluidic operation device according to claim 9, characterized in that: The magnetic attraction control module includes: a magnetic attraction component for adsorbing the nucleic acid-magnetic bead complex on the microfluidic chip to control the flow direction of the nucleic acid-magnetic bead complex, a first driving component for controlling the linear movement of the magnetic attraction component along a first direction, a second driving component for controlling the linear movement of the magnetic attraction component along a second direction, and a third driving component for controlling the linear movement of the magnetic attraction component along a third direction; The magnetic attraction component is transmission-connected to the driving end of the first driving component; the first driving component is transmission-connected to the driving end of the second driving component; the second driving component is transmission-connected to the driving end of the third driving component; the third driving component is transmission-connected to the driving end of the first linear module; The first drive component, the second drive component and the third drive component are all communicatively connected to the control module.

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