Cell filter convenient for separation and filtration and filtration method thereof

Abstract

The invention relates to the technical field of cell filtration, and discloses a cell filter facilitating separation and filtration and a filtration method thereof.The cell filter comprises a main body, a fixing ring is fixedly connected to the interior of the main body, a cross-shaped spring frame is slidably connected to the outer surface of the fixing ring, and a conical spiral block is fixedly connected to the top of the cross-shaped spring frame; three fixing frames are fixedly connected to the side, away from the conical spiral block, of the cross spring frame, the three fixing frames are arrayed circumferentially with the conical spiral block as the center, and C-shaped rings are fixedly connected to the sides, away from the conical spiral block, of the three fixing frames. Meanwhile, when the air injection ring irregularly shakes, cell sap on the inner wall of the bottom of the main body can be continuously disturbed, so that cells and solutes in the cell sap can be more uniformly distributed on the surface of the filter screen, and the condition that the filtering resistance is too large due to local cell accumulation is avoided, so that the filtering effect is improved.

Description

Technical Field

[0001] The invention relates to the technical field of cell filtration, in particular to a cell filter convenient for separation and filtration and a filtration method thereof. Background Art

[0002] In the field of cell biology research, whether it is basic research such as the exploration of cell physiological functions, or applied research such as cell therapy and drug screening, cells need to be separated and filtered. For example, when studying the characteristics of cell subpopulations, it is necessary to separate specific types of cells from complex tissue samples;

[0003] Generally, when filtering cells, the cell fluid is basically placed in the filter and the cells are filtered by relying on the downward flow of the liquid itself. Since the existing devices basically rely on the natural flow of the liquid to filter the cells, it is easy to cause cell aggregation and accumulation of the cell fluid containing cells during filtration. When the cells aggregate, it is easy to cause excessive resistance on the filter surface, affecting the filtration speed and filtration efficiency of the cells. Summary of the invention

[0004] The object of the present invention is to provide a cell filter and a filtering method thereof which are convenient for separation and filtration, so as to solve the problems raised in the above-mentioned background technology.

[0005] In order to solve the above technical problems, the present invention is achieved through the following technical solutions:

[0006] The present invention is a cell filter for separation and filtration, comprising a main body, a fixing ring fixedly connected inside the main body, a cross spring frame slidably connected to the outer surface of the fixing ring, a conical spiral block fixedly connected to the top of the cross spring frame, three fixing frames fixedly connected to the side of the cross spring frame away from the conical spiral block, the three fixing frames are arranged in a circle with the conical spiral block as the center, and a C-shaped ring is fixedly connected to the side of the three fixing frames away from the conical spiral block, and further comprising:

[0007] The transmission mechanism includes a fan shaft rotatably connected to a side of the cross spring frame close to the fixed frame, the outer surface of the fan shaft is rotatably connected to a working frame, the outer surface of the working frame is fixedly connected between the three fixed frames, the top inner wall of the working frame is fixedly connected to a fixed cylinder, and the inner wall of the fixed cylinder is provided with a wave groove;

[0008] The shaking mechanism includes a plurality of hollow frames rotatably connected to the outer surface of the fan shaft inside the working frame, a T-shaped plate is slidably connected to the inside of the hollow frame, the side wall of the T-shaped plate is slidably connected to the inside of the wave groove, a sliding frame is slidably connected to the side of the plurality of hollow frames away from the fan shaft, a diaphragm is fixedly connected to the bottom of the sliding frame, and the outer surface of the diaphragm is fixedly connected to the inside of the working frame.

[0009] Furthermore, a gear cylinder is fixedly connected to one side of the working frame close to the C-shaped ring, a grille ring is fixedly connected to the outer surface of the fan shaft inside the working frame, a plurality of inclined plates are fixedly connected to the outer surface of the fan shaft inside the grille ring, and a conical cylinder is fixedly connected to the bottom inner wall of the fan shaft.

[0010] Furthermore, a cleaning mechanism is provided inside the main body, and the cleaning mechanism includes a plurality of one-way tubes fixedly connected to the outer surface of the working frame, the one-way tubes are connected to the working frame at one side close to the working frame, and the ends of the plurality of one-way tubes away from the working frame are fixedly connected to the side wall of the C-shaped ring, the interior of the C-shaped ring is connected to the one-way tubes, and a fan disk is provided on the one side of the one-way tubes close to the C-shaped ring, and the fan disk is rotatably connected to the interior of the C-shaped ring.

[0011] Furthermore, a side of the fan disk away from the one-way tube is rotatably connected to a plurality of curved jet tubes, an end of the plurality of curved jet tubes away from the fan disk is rotatably connected to an elastic ring, a hose is fixedly connected to the top of the curved jet tube, the top of the hose is fixedly connected to the side wall of the fan disk near the C-shaped ring, the hose is connected to the interior of the C-shaped ring, and a plurality of intermediate rods are rotatably connected to the side of the elastic ring near the fan shaft.

[0012] Furthermore, an auxiliary mechanism is arranged inside the main body, and the auxiliary mechanism includes an intermediate tube fixedly connected to the outer surface of the one-way tube, and one end of a plurality of intermediate tubes away from the one-way tube is fixedly connected to an air jet ring.

[0013] Furthermore, the jet ring is connected to the one-way tube through the intermediate tube, and the outer surface of the jet ring is rotatably connected to a plurality of limit rings, the side of the limit ring close to the intermediate rod is rotatably connected to the intermediate rod, and the side of the limit ring close to the fan shaft is fixedly connected to a spring rod.

[0014] Furthermore, a blocking mechanism is provided inside the main body, and the blocking mechanism includes an obtuse-angled plate rotatably connected to the end of the spring rod away from the limiting ring, the end of the obtuse-angled plate close to the fan shaft is rotatably connected to the outer surface of the fan shaft, the side wall of the obtuse-angled plate is fixedly connected to a spring, and the end of the spring away from the obtuse-angled plate is rotatably connected to the outer surface of the fan shaft.

[0015] Furthermore, the end of the obtuse-angled plate away from the fan shaft is rotatably connected to a flexible shaft, the end of the flexible shaft close to the fan shaft is fixedly connected to a gear, the end of the gear close to the fan shaft is rotatably connected to the outer surface of the fan shaft, and the outer surface of the gear is meshingly connected to the bottom of the gear cylinder.

[0016] Furthermore, a method for using a cell filter for separation and filtration is provided, the method comprising the following steps:

[0017] S1: First, place the device inside the main body, then place the main body at the entrance of the collection container, and then the staff pours the solution containing cell fluid onto the conical spiral block inside the main body;

[0018] S2: The liquid will then flow into the main body along the conical spiral block. When the liquid is poured into the main body, the liquid will be filtered through the bottom of the main body and enter the collection container to complete the filtering purpose.

[0019] The present invention has the following beneficial effects:

[0020] 1. In the present invention, when the liquid flows downward through the spiral groove on the conical spiral block, the cell fluid will flow in a spiral along the spiral groove on the surface of the conical spiral block. When the liquid flows downward in a spiral flow manner, the flow speed of the cell fluid flowing downward can be increased and impact the fan shaft. When the cell fluid impacts the fan shaft, the speed of the liquid flowing downward will impact the fan shaft and cause it to rotate. When the fan shaft rotates, it will drive a plurality of inclined plates on its surface to rotate, and the plurality of inclined plates will adsorb external gas when rotating. And blow it into the working frame through the conical cylinder at the bottom. Then, when the fan shaft rotates, the rotation of the fan shaft will drive the T-plate to rotate through the hollow frame. When the T-plate rotates, it will slide up and down in the wave groove on the inner wall of the fixed cylinder. When the T-plate slides up and down, the sliding of the T-plate will drive the hollow frame to rotate back and forth. When the hollow frame rotates, it will drive the diaphragm to swing back and forth through the sliding frame. When the diaphragm swings upward, it will absorb the external gas through the conical cylinder at the bottom. Combined with the rotation of the inclined plate, the external gas will be The gas is adsorbed into the working frame, and then when the diaphragm swings downward, the swing of the diaphragm will squeeze the gas. At this time, the gas will enter the one-way tube after being squeezed. At the same time, when the fan shaft rotates, the rotation of the fan shaft will drive the grille ring to rotate synchronously. When the grille ring rotates, it will generate an outward swinging force on the gas and, combined with the downward swinging of the diaphragm, the gas can enter the one-way tube at an accelerated speed. Then, most of the gas will enter the jet ring through the intermediate tube and be ejected outward through the jet ring. At this time, at the moment of gas ejection, the jet ring will shake irregularly on the bottom inner wall of the main body. When the gas is ejected, the impact force generated by the ejected gas on the bottom inner wall of the main body can destroy the liquid film resistance that may be formed by the cell sap on the bottom inner wall of the main body, making it easier for the cell sap to pass through the filter. At the same time, when the jet ring shakes irregularly, it can continuously disturb the cell sap on the bottom inner wall of the main body, so that the cells and solutes in the cell sap can be more evenly distributed on the filter surface, avoiding the situation where the filtration resistance is too large due to local cell accumulation, thereby improving the filtration effect.

[0021] 2. In the present invention, when the gas enters the one-way tube, the gas will enter the C-shaped ring through the one-way tube, and then the fast-flowing gas will impact the fan disk when entering the C-shaped ring. When the fan disk is impacted, the fan disk will drive multiple curved jet tubes to rotate. Then, when the fan disk drives the curved jet tube to rotate, the gas inside the C-shaped ring will enter the curved jet tube through the hose and be ejected outward through the curved jet tube. At this time, when the gas is ejected, the gas will be sprayed to the inner wall of the main body and the corner of the inner wall at the bottom of the main body as the curved jet tube rotates, which can prevent the shaking of the cell jet ring from disturbing the cells, causing the cells to hang on the wall and push at the inner wall and corner of the main body and gradually form blockage. At the same time, when the curved jet tube rotates and ejects gas, the rotating gas can make the cells accumulated on the inner wall and edge of the bottom of the main body blow downward, so that part of the cell fluid returns to the mainstream of the cell fluid, thereby reducing the possibility of filter blockage and residue, and improving the filtration efficiency.

[0022] 3. According to the present invention, when the fan shaft rotates, the rotation of the fan shaft will drive multiple obtuse-angled plates and the flexible shaft to rotate synchronously. When the flexible shaft rotates, the gear on the flexible shaft will mesh with the teeth at the bottom of the gear cylinder and rotate at the bottom of the gear cylinder when the fan shaft rotates. When the flexible shaft rotates, the rotation of the flexible shaft will drive the obtuse-angled plate to rotate up and down. When the obtuse-angled plate rotates downward, the rotation of the obtuse-angled plate will pull the limit ring through the spring rod. After the limit ring is pulled by the spring rod, the pulling of the limit ring will drive the elastic ring to move synchronously through the middle rod. When the elastic ring is pulled by the middle rod, the elastic ring will drive multiple curved jet pipes to rotate at the bottom of the fan disk. When the curved jet pipe rotates, the airflow will be ejected downward. This downward jet direction can effectively suppress the upward splashing of cell fluid when the jet ring shakes and the gas is ejected, thereby reducing the possibility of cell fluid splashing and improving the accuracy of the filtration process.

[0023] 4. In the present invention, when the fan shaft drives multiple obtuse-angle plates and the flexible shaft to rotate, the rotation of the fan shaft will rotate at the bottom of the gear cylinder through the gear on the obtuse-angle plate. Since the gear and the bottom of the gear cylinder are meshed, when the fan shaft rotates, it will drive the gear to rotate synchronously. When the gear rotates, it will drive the flexible shaft to shake. When the flexible shaft shakes, it will drive the obtuse-angle plate to shake up and down on the surface of the fan shaft. Then, when the rotation of the fan shaft drives the obtuse-angle plate to rotate, the obtuse-angle plate will rotate in the middle of the jet ring. When the obtuse-angle plate rotates in the middle of the jet ring, the rotation of the obtuse-angle plate will disturb the gas ejected through the jet ring. After the airflow is disturbed by the obtuse-angle plate, the flow velocity of the gas ejection can be reduced, and the fast-flowing airflow can be avoided from causing direct impact on the cells, thereby reducing the mechanical damage to the cells, so as to protect the integrity and activity of the cells.

[0024] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for describing the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative work.

[0026] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0027] Figure 2 It is a schematic diagram of the overall partial cross-sectional structure of the present invention;

[0028] Figure 3 It is a schematic diagram of the internal structure of the main body of the present invention;

[0029] Figure 4 It is a schematic diagram of the partial cross-sectional structure of the main body of the present invention;

[0030] Figure 5 It is a schematic diagram of the transmission mechanism structure of the present invention;

[0031] Figure 6 It is a schematic diagram of a partial cross-sectional structure of the transmission mechanism of the present invention;

[0032] Figure 7 It is a schematic diagram of the internal structure of the transmission mechanism of the present invention;

[0033] Figure 8 It is a schematic diagram of the cleaning mechanism structure of the present invention;

[0034] Fig. 9 It is a schematic diagram of the auxiliary mechanism structure of the present invention;

[0035] Fig.10 This is a schematic diagram of the one-way tube structure of the present invention;

[0036] Fig.11 The figure is a flow chart of the filtering method of the present invention.

[0037] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0038] In the figure: 1. main body; 101. fixed ring; 102. cross spring frame; 103. conical spiral block; 104. fixed frame; 105. C-ring; 2. transmission mechanism; 201. fan shaft; 202. working frame; 203. fixed cylinder; 204. gear cylinder; 205. grille ring; 3. shaking mechanism; 301. hollow frame; 302. T-plate; 303. sliding frame; 304. diaphragm; 4. cleaning mechanism; 401. one-way pipe; 402. fan disk; 403. curved jet pipe; 404. elastic ring; 405. middle rod; 5. auxiliary mechanism; 501. middle tube; 502. jet ring; 503. limit ring; 504. spring rod; 6. blocking mechanism; 601. obtuse plate; 602. flexible shaft. DETAILED DESCRIPTION

[0039] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] See also Figure 1-Figure 10 As shown, the present invention is a cell filter for separation and filtration, comprising a main body 1, a fixing ring 101 is fixedly connected inside the main body 1, a cross spring frame 102 is slidably connected to the outer surface of the fixing ring 101, a conical spiral block 103 is fixedly connected to the top of the cross spring frame 102, three fixing frames 104 are fixedly connected to the side of the cross spring frame 102 away from the conical spiral block 103, the three fixing frames 104 are arranged in a circle with the conical spiral block 103 as the center, and a C-shaped ring 105 is fixedly connected to the side of the three fixing frames 104 away from the conical spiral block 103, and further comprising;

[0041] The transmission mechanism 2 includes a fan shaft 201 rotatably connected to a side of the cross spring frame 102 close to the fixed frame 104, the outer surface of the fan shaft 201 is rotatably connected to a working frame 202, the outer surface of the working frame 202 is fixedly connected between the three fixed frames 104, the top inner wall of the working frame 202 is fixedly connected to a fixed cylinder 203, and the inner wall of the fixed cylinder 203 is provided with a wave groove;

[0042] The shaking mechanism 3 includes a plurality of hollow frames 301 rotatably connected to the outer surface of the fan shaft 201 located inside the working frame 202, a T-plate 302 is slidably connected inside the hollow frame 301, and the side wall of the T-plate 302 is slidably connected to the inside of the wave groove, and a sliding frame 303 is slidably connected to the side of the plurality of hollow frames 301 away from the fan shaft 201, and a diaphragm 304 is fixedly connected to the bottom of the sliding frame 303, and the outer surface of the diaphragm 304 is fixedly connected to the inside of the working frame 202. First, the device is placed inside the main body 1, and then the main body 1 is placed at the entrance of the collection container. Subsequently, the staff pours the solution containing the cell fluid onto the conical spiral block 103 inside the main body 1, and then the liquid flows into the main body 1 along the conical spiral block 103. When the liquid is poured into the main body 1, the liquid is filtered through the bottom of the main body 1 and enters the collection container to complete the filtering purpose.

[0043] A gear cylinder 204 is fixedly connected to one side of the working frame 202 close to the C-shaped ring 105, a grille ring 205 is fixedly connected to the outer surface of the fan shaft 201 inside the working frame 202, a plurality of inclined plates are fixedly connected to the outer surface of the fan shaft 201 inside the grille ring 205, and a conical cylinder is fixedly connected to the bottom inner wall of the fan shaft 201. When the liquid flows downward through the spiral groove on the conical spiral block 103, the cell fluid will flow in a spiral along the spiral groove on the surface of the conical spiral block 103. When the liquid flows downward in a spiral manner, the downward flow speed of the cell fluid can be increased and impact the fan shaft 201. When the cell fluid impacts the fan shaft 201, the downward flow speed of the liquid will impact the fan shaft 201 and cause it to rotate.

[0044] A cleaning mechanism 4 is arranged inside the main body 1, and the cleaning mechanism 4 includes a plurality of one-way tubes 401 fixedly connected to the outer surface of the working frame 202, the one side of the one-way tube 401 close to the working frame 202 is connected to the working frame 202, and the ends of the plurality of one-way tubes 401 away from the working frame 202 are fixedly connected to the side wall of the C-shaped ring 105, and the interior of the C-shaped ring 105 is connected to the one-way tube 401, and a fan disk 402 is arranged on the one side of the one-way tube 401 close to the C-shaped ring 105, and the fan disk 402 is rotatably connected to the interior of the C-shaped ring 105. When gas enters the one-way tube 401, the gas will enter the C-shaped ring 105 through the one-way tube 401, and then the fast-flowing gas will impact the fan disk 402 when entering the C-shaped ring 105.

[0045] A side of the fan disk 402 away from the one-way tube 401 is rotatably connected to a plurality of curved jet tubes 403, and one end of the plurality of curved jet tubes 403 away from the fan disk 402 is rotatably connected to an elastic ring 404, and a hose is fixedly connected to the top of the curved jet tube 403, and the hose is fixedly connected to the side wall of the fan disk 402 near the top of the C-shaped ring 105, and the hose is connected to the inside of the C-shaped ring 105, and a side of the elastic ring 404 close to the fan shaft 201 is rotatably connected to a plurality of intermediate rods 405, and when the fan disk 402 is impacted, the fan disk 402 will drive the plurality of curved jet tubes 403 to rotate, and then when the fan disk 402 drives the curved jet tube 403 to rotate, the gas inside the C-shaped ring 105 will enter the curved jet tube 403 through the hose and be ejected outward through the curved jet tube 403.

[0046] An auxiliary mechanism 5 is provided inside the main body 1, and the auxiliary mechanism 5 includes an intermediate tube 501 fixedly connected to the outer surface of the one-way tube 401, and one end of several intermediate tubes 501 away from the one-way tube 401 is fixedly connected to a jet ring 502, and then most of the gas will enter the jet ring 502 through the intermediate tube 501 and be ejected outward through the jet ring 502. At this time, at the moment of gas ejection, the jet ring 502 will shake irregularly on the bottom inner wall of the main body 1.

[0047] The jet ring 502 is connected to the one-way tube 401 through the intermediate tube 501. The outer surface of the jet ring 502 is rotatably connected to a plurality of limit rings 503. The side of the limit ring 503 close to the intermediate rod 405 is rotatably connected to the intermediate rod 405. The side of the limit ring 503 close to the fan shaft 201 is fixedly connected to a spring rod 504. When the position ring 503 is pulled by the spring rod 504, the pulling of the limit ring 503 will drive the elastic ring 404 to move synchronously through the intermediate rod 405.

[0048] A blocking mechanism 6 is provided inside the main body 1, and the blocking mechanism 6 includes an obtuse-angled plate 601 rotatably connected to the end of the spring rod 504 away from the limit ring 503, and the end of the obtuse-angled plate 601 close to the fan shaft 201 is rotatably connected to the outer surface of the fan shaft 201, and the side wall of the obtuse-angled plate 601 is fixedly connected with a spring, and the end of the spring away from the obtuse-angled plate 601 is rotatably connected to the outer surface of the fan shaft 201. When the fan shaft 201 drives multiple obtuse-angled plates 601 and the flexible shaft 602 to rotate, the rotation of the fan shaft 201 will rotate at the bottom of the gear cylinder 204 through the gear on the obtuse-angled plate 601. Since the gear is meshed with the bottom of the gear cylinder 204, when the fan shaft 201 rotates, it will drive the gear to rotate synchronously. When the gear rotates, it will drive the flexible shaft 602 to shake, and when the flexible shaft 602 shakes, it will drive the obtuse-angled plate 601 to shake up and down on the surface of the fan shaft 201.

[0049] One end of the obtuse-angled plate 601 away from the fan shaft 201 is rotatably connected to the flexible shaft 602, and one end of the flexible shaft 602 close to the fan shaft 201 is fixedly connected to a gear. The end of the gear close to the fan shaft 201 is rotatably connected to the outer surface of the fan shaft 201, and the outer surface of the gear is meshed with the bottom of the gear cylinder 204. When the rotation of the fan shaft 201 drives the obtuse-angled plate 601 to rotate, the rotation of the obtuse-angled plate 601 will rotate in the middle of the jet ring 502. When the obtuse-angled plate 601 rotates in the middle of the jet ring 502, the rotation of the obtuse-angled plate 601 will disturb the gas ejected through the jet ring 502.

[0050] A method for using a cell filter for separation and filtration, the method comprising the following steps:

[0051] S1: First, the device is placed inside the main body 1, and then the main body 1 is placed at the entrance of the collection container, and then the staff pours the solution containing the cell fluid onto the conical spiral block 103 inside the main body 1;

[0052] S2: The liquid will then flow into the main body 1 along the conical spiral block 103. When the liquid is poured into the main body 1, the liquid will be filtered through the bottom of the main body 1 and enter the collection container, thus completing the filtering purpose.

[0053] When in use, first place this device inside the main body 1, then place the main body 1 at the entrance of the collection container, and then the staff will pour the solution containing cell fluid onto the conical spiral block 103 inside the main body 1, and then the liquid will flow into the main body 1 along the conical spiral block 103. When the liquid is poured into the main body 1, the liquid will be filtered through the bottom of the main body 1 and enter the collection container to complete the filtering purpose.

[0054] When the liquid flows downward through the spiral grooves on the conical spiral block 103, the cell fluid will flow in a spiral along the spiral grooves on the surface of the conical spiral block 103. When the liquid flows downward in a spiral manner, the flow speed of the cell fluid flowing downward can be increased and the fan shaft 201 can be impacted. When the cell fluid impacts the fan shaft 201, the speed of the liquid flowing downward will impact the fan shaft 201 and cause it to rotate. When the fan shaft 201 rotates, it will drive several inclined plates on its surface to rotate. When the several inclined plates rotate, they will absorb the external gas and blow it to the working part through the conical tube at the bottom. The fan shaft 201 is in the frame 202, and then when the fan shaft 201 rotates, the rotation of the fan shaft 201 will drive the T-plate 302 to rotate through the hollow frame 301. When the T-plate 302 rotates, it will slide up and down in the wave groove on the inner wall of the fixed cylinder 203. When the T-plate 302 slides up and down, the sliding of the T-plate 302 will drive the hollow frame 301 to rotate back and forth. When the hollow frame 301 rotates, it will drive the diaphragm 304 to shake back and forth through the sliding frame 303. When the diaphragm 304 shakes upward, it will absorb the external gas through the conical cylinder at the bottom, and combined with the rotation of the inclined plate, it will The external gas is adsorbed into the working frame 202, and then when the diaphragm 304 swings downward, the swing of the diaphragm 304 will squeeze the gas, and the gas will enter the one-way tube 401 after being squeezed. At the same time, when the fan shaft 201 rotates, the rotation of the fan shaft 201 will drive the grille ring 205 to rotate synchronously. When the grille ring 205 rotates, it will generate an outward swinging force on the gas, and combined with the downward swinging of the diaphragm 304, the gas can be accelerated to enter the one-way tube 401, and then most of the gas will enter the jet ring 502 through the intermediate tube 501 and go out through the jet ring 502. At the moment of gas ejection, the jet ring 502 will shake irregularly on the bottom inner wall of the main body 1. When the gas is ejected, the impact force generated by the ejected gas on the bottom inner wall of the main body 1 can destroy the liquid film resistance that may be formed by the cell fluid on the bottom inner wall of the main body 1, making it easier for the cell fluid to pass through the filter. At the same time, when the jet ring 502 shakes irregularly, it can continuously disturb the cell fluid on the bottom inner wall of the main body 1, so that the cells and solutes in the cell fluid can be more evenly distributed on the filter surface, avoiding the situation where the filtration resistance is too large due to local cell accumulation, thereby improving the filtration effect.

[0055] When the gas enters the one-way tube 401, the gas will enter the C-shaped ring 105 through the one-way tube 401. Then, when the fast-flowing gas enters the C-shaped ring 105, the gas will impact the fan disk 402. When the fan disk 402 is impacted, the fan disk 402 will drive the multiple curved jet tubes 403 to rotate. Then, when the fan disk 402 drives the curved jet tubes 403 to rotate, the gas inside the C-shaped ring 105 will enter the curved jet tubes 403 through the hose and be ejected outward through the curved jet tubes 403. At this time, when the gas is ejected, the gas will follow the curved jet tubes 403. The rotation of the jet tube 403 sprays toward the inner wall of the main body 1 and the corner of the inner wall at the bottom of the main body 1, which can prevent the cell jet ring 502 from shaking, causing the shaking jet ring 502 to disturb the cells, causing the cells to hang on the inner wall and corners of the main body 1 and push against the wall and gradually form a blockage. At the same time, when the curved jet tube 403 rotates and sprays gas, the rotating gas can blow the cells accumulated on the inner wall and edge of the bottom of the main body 1 downward, so that part of the cell fluid returns to the mainstream of the cell fluid, thereby reducing the possibility of filter blockage and residue, and improving the filtration efficiency.

[0056] When the fan shaft 201 is rotating, the rotation of the fan shaft 201 will drive the multiple obtuse-angled plates 601 and the flexible shaft 602 to rotate synchronously. When the flexible shaft 602 is rotating, the gears on the flexible shaft 602 will mesh with the teeth at the bottom of the gear cylinder 204 and rotate at the bottom of the gear cylinder 204 when the fan shaft 201 rotates. When the flexible shaft 602 is rotating, the rotation of the flexible shaft 602 will drive the obtuse-angled plates 601 to rotate up and down. When the obtuse-angled plates 601 rotate downward, the rotation of the obtuse-angled plates 601 will pull the limit ring 503 through the spring rod 504, and the limit ring 503 will rotate upward. After being pulled by the spring rod 504, the pulling of the limit ring 503 will drive the elastic ring 404 to move synchronously through the middle rod 405. When the elastic ring 404 is pulled by the middle rod 405, the elastic ring 404 will drive multiple curved jet tubes 403 to rotate at the bottom of the fan disk 402. When the curved jet tubes 403 rotate, the airflow will be ejected downward. This downward jet direction can effectively suppress the shaking of the jet ring 502 and the upward splashing of the gas when it is ejected, thereby reducing the possibility of cell fluid splashing and improving the accuracy of the filtration process.

[0057] When the fan shaft 201 drives the multiple obtuse-angled plates 601 and the flexible shaft 602 to rotate, the rotation of the fan shaft 201 will rotate at the bottom of the gear cylinder 204 through the gears on the obtuse-angled plates 601. Since the gears are meshed with the bottom of the gear cylinder 204, when the fan shaft 201 rotates, the gears will rotate synchronously. When the gears rotate, the flexible shaft 602 will shake. When the flexible shaft 602 shakes, it will drive the obtuse-angled plates 601 to shake up and down on the surface of the fan shaft 201. When the rotation drives the obtuse-angled plate 601 to rotate, the rotation of the obtuse-angled plate 601 will rotate in the middle of the jet ring 502. When the obtuse-angled plate 601 rotates in the middle of the jet ring 502, the rotation of the obtuse-angled plate 601 will disturb the gas ejected through the jet ring 502. After the airflow passes through the disturbance of the obtuse-angled plate 601, the flow speed of the gas ejection can be reduced, and the fast-flowing airflow can be avoided. Direct impact on the cells, thereby reducing the mechanical damage caused to the cells, so as to protect the integrity and activity of the cells.

[0058] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A cell filter for separation and filtration, comprising a main body (1), wherein a fixing ring (101) is fixedly connected inside the main body (1), a cross spring frame (102) is slidably connected to the outer surface of the fixing ring (101), a conical spiral block (103) is fixedly connected to the top of the cross spring frame (102), three fixing frames (104) are fixedly connected to the side of the cross spring frame (102) away from the conical spiral block (103), the three fixing frames (104) are arranged in a circular array with the conical spiral block (103) as the center, and a C-shaped ring (105) is fixedly connected to the side of the three fixing frames (104) away from the conical spiral block (103), characterized in that: Also includes; A transmission mechanism (2), the transmission mechanism (2) comprising a fan shaft (201) rotatably connected to a side of a cross spring frame (102) close to a fixed frame (104), an outer surface of the fan shaft (201) being rotatably connected to a working frame (202), an outer surface of the working frame (202) being fixedly connected between three fixed frames (104), a top inner wall of the working frame (202) being fixedly connected to a fixed cylinder (203), an inner wall of the fixed cylinder (203) being provided with a wave groove; A shaking mechanism (3), the shaking mechanism (3) comprising a plurality of hollow frames (301) rotatably connected to the outer surface of the fan shaft (201) located inside the working frame (202), a T-shaped plate (302) being slidably connected inside the hollow frame (301), a side wall of the T-shaped plate (302) being slidably connected inside the wave groove, a sliding frame (303) being slidably connected on one side of the plurality of hollow frames (301) away from the fan shaft (201), a diaphragm (304) being fixedly connected at the bottom of the sliding frame (303), and an outer surface of the diaphragm (304) being fixedly connected inside the working frame (202).

2. A cell filter for separation and filtration according to claim 1, characterized in that: A gear cylinder (204) is fixedly connected to one side of the working frame (202) close to the C-shaped ring (105); a grille ring (205) is fixedly connected to the outer surface of the fan shaft (201) inside the working frame (202); a plurality of inclined plates are fixedly connected to the outer surface of the fan shaft (201) inside the grille ring (205); and a conical cylinder is fixedly connected to the bottom inner wall of the fan shaft (201).

3. A cell filter for separation and filtration according to claim 2, characterized in that: A cleaning mechanism (4) is arranged inside the main body (1), and the cleaning mechanism (4) comprises a plurality of one-way tubes (401) fixedly connected to the outer surface of the working frame (202), the one-way tubes (401) being connected to the working frame (202) at one side close to the working frame (202), the one-way tubes (401) being fixedly connected to the side wall of the C-shaped ring (105) at one end away from the working frame (202), the interior of the C-shaped ring (105) being connected to the one-way tubes (401), the one-way tubes (401) being connected to one side close to the C-shaped ring (105), and the fan disk (402) being rotatably connected to the interior of the C-shaped ring (105).

4. A cell filter for separation and filtration according to claim 3, characterized in that: A side of the fan disc (402) away from the one-way tube (401) is rotatably connected to a plurality of curved jet pipes (403); one end of the plurality of curved jet pipes (403) away from the fan disc (402) is rotatably connected to an elastic ring (404); a top of the curved jet pipe (403) is fixedly connected to a hose; a top of the hose near the C-shaped ring (105) is fixedly connected to the side wall of the fan disc (402); the hose is connected to the inside of the C-shaped ring (105); and a side of the elastic ring (404) near the fan shaft (201) is rotatably connected to a plurality of intermediate rods (405).

5. A cell filter for separation and filtration according to claim 4, characterized in that: An auxiliary mechanism (5) is arranged inside the main body (1), and the auxiliary mechanism (5) comprises an intermediate tube (501) fixedly connected to the outer surface of the one-way tube (401), and a plurality of intermediate tubes (501) are fixedly connected to an air jet ring (502) at one end away from the one-way tube (401).

6. A cell filter for separation and filtration according to claim 5, characterized in that: The jet ring (502) is connected to the one-way tube (401) through the intermediate tube (501); the outer surface of the jet ring (502) is rotatably connected to a plurality of limiting rings (503); the limiting ring (503) is rotatably connected to the intermediate rod (405) at one side thereof; and the limiting ring (503) is fixedly connected to a spring rod (504) at one side thereof that is close to the fan shaft (201).

7. A cell filter for separation and filtration according to claim 6, characterized in that: A blocking mechanism (6) is arranged inside the main body (1), and the blocking mechanism (6) comprises an obtuse-angled plate (601) rotatably connected to an end of the spring rod (504) away from the limiting ring (503), an end of the obtuse-angled plate (601) close to the fan shaft (201) is rotatably connected to the outer surface of the fan shaft (201), and a spring is fixedly connected to the side wall of the obtuse-angled plate (601), and an end of the spring away from the obtuse-angled plate (601) is rotatably connected to the outer surface of the fan shaft (201).

8. A cell filter for separation and filtration according to claim 7, characterized in that: One end of the obtuse-angled plate (601) away from the fan shaft (201) is rotatably connected to a flexible shaft (602), and one end of the flexible shaft (602) close to the fan shaft (201) is fixedly connected to a gear, and one end of the gear close to the fan shaft (201) is rotatably connected to the outer surface of the fan shaft (201), and the outer surface of the gear is meshingly connected to the bottom of the gear cylinder (204).

9. A method for using a cell filter for separation and filtration, characterized in that: Using the cell filter for separation and filtration as claimed in claim 8, the method comprises the following steps: S1: First, the device is placed inside the main body (1), and then the main body (1) is placed at the entrance of the collection container, and then the staff pours the solution containing the cell fluid onto the conical spiral block (103) inside the main body (1); S2: The liquid then flows along the conical spiral block (103) into the main body (1). When the liquid is poured into the main body (1), the liquid is filtered through the bottom of the main body (1) and enters the collection container, thereby completing the filtering purpose.

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