Composition for promoting cell regeneration and application thereof

Abstract

The invention relates to a composition for promoting cell regeneration and application thereof, and particularly provides a composition for promoting cell regeneration, the composition comprises L-tryptophan oxalate, isosorbide-2-acetate and glabridin, and the composition is applied to preparation of a cell regeneration agent and construction of a cell model. The participation of the composition can effectively improve the proliferation capacity of cells and the expression level of CD63 in the cells. The method not only effectively improves the regeneration capacity of cells, but also is simple to operate and low in cost, and can be applied to the fields of preparation of cosmetics, skin care products, wound repair materials or medicines and skin repair.

Description

Technical Field

[0001] The invention belongs to a composition having a beneficial effect on skin, and in particular relates to a composition for promoting cell regeneration and an application thereof. Background Art

[0002] At present, for women, the gradual aging of the skin is undoubtedly an unpleasant loss of elegance. Skin aging is a phenomenon of skin aging caused by natural or unnatural factors. After a person is born, the skin tissue becomes increasingly developed and its functions become increasingly active. When it reaches a certain age, it will begin to degenerate. This is a phenomenon of skin aging caused by natural factors. The unnatural factors that cause skin aging include: ultraviolet radiation, pollution, emotions and stress. In addition, there are many reasons for skin damage, such as burns, genetic diseases, chronic trauma, etc. Among them, according to surveys, about 26 million people in China suffer from burns of varying degrees every year, accounting for 2% of the total population, and the number of deaths is second only to traffic accidents. Among them, burns and scalds in children aged 0-12 account for 30%-50%, and this proportion is showing a continuous growth trend. In addition, trauma caused by diabetes can also cause skin damage. Amputations caused by diabetic foot account for 27.3% of all amputations, and the 5-year mortality rate of patients after amputation is as high as 40.0%. Foot ulcers are common in diabetics. Due to the microenvironment of diabetics, this skin ulcer is difficult to heal and there is no better treatment. Therefore, many human skin damages cannot be restored by themselves, and large-area skin damages are very likely to cause infection and lead to death. Therefore, inventing a composition that has a beneficial effect on the skin is of great significance in the field of skin repair. Summary of the invention

[0003] The purpose of the present invention is to provide a composition for promoting cell regeneration and its application.

[0004] The technical solution adopted by the present invention to achieve the above-mentioned purpose is: A composition comprises at least one of L-tryptophanol oxalate and isosorbide-2-acetate. Among them, when the composition is L-tryptophanol oxalate and isosorbide-2-acetate, when it is added to F12 complete culture medium as a cell regeneration agent for cell culture, the use of L-tryptophanol oxalate and isosorbide-2-acetate significantly increases the expression level of CD63 in the cells, indicating that L-tryptophanol oxalate and isosorbide-2-acetate can effectively increase the secretion of exosomes, and at the same time can effectively improve the wound recovery ability and promote the regeneration ability of cells; in addition, when the composition is a single L-tryptophanol oxalate or isosorbide-2-acetate, when it is added to F12 complete culture medium as a cell regeneration agent for cell culture, the use of a single L-tryptophanol oxalate or isosorbide-2-acetate has no obvious effect on improving the expression level of CD63 in the cells, indicating that the use of a single L-tryptophanol oxalate or isosorbide-2-acetate cannot effectively increase the secretion of exosomes, and has no obvious promoting effect on wound recovery ability and cell regeneration ability.

[0005] Preferably, L-tryptophanol oxalate and isosorbide-2-acetate are mixed in a mass ratio of 1:1 to 4.

[0006] Preferably, a composition comprises: at least one of L-tryptophanol oxalate, isosorbide-2-acetate and glabridol.

[0007] More preferably, the composition comprises L-tryptophanol oxalate.

[0008] More preferably, the composition comprises L-tryptophanol oxalate and isosorbide-2-acetate.

[0009] More preferably, the mass ratio of L-tryptophanol oxalate to isosorbide-2-acetate is 1:1-4.

[0010] More preferably, the composition includes L-tryptophanol oxalate, isosorbide-2-acetate and glabripenol. Among them, when the composition is glabripenol, when it is added to F12 complete medium containing L-tryptophanol oxalate and isosorbide-2-acetate as a cell regeneration agent for cell culture, the use of glabripenol further significantly increases the expression level of CD63 in the cells, indicating that glabripenol can further effectively increase the secretion of exosomes, and at the same time can effectively improve the wound recovery ability and promote the regeneration ability of cells.

[0011] More preferably, the mass ratio of L-tryptophanol oxalate, isosorbide-2-acetate and glabriquitin is 1:1-4:1-4.

[0012] The present invention also provides a cell regeneration agent, comprising: the above composition.

[0013] Preferably, the cell regeneration agent further comprises F12 complete medium, and the concentration of the composition in the F12 complete medium is 0.002-0.008 g / ml.

[0014] The present invention also provides a method for constructing a cell model, comprising: using the above-mentioned cell regeneration agent to culture cells to obtain a cell model.

[0015] Preferably, the cells include any one of hematopoietic stem cells, adipose stem cells, umbilical cord blood stem cells, and umbilical cord mesenchymal stem cells.

[0016] Preferably, the exosomes secreted by the cells in the cell model contain at least one of nucleic acid, protein and cholesterol.

[0017] More preferably, the surface marker of exosomes includes at least one of CD63, CD81, CD9, TSG101 and HSP27.

[0018] Preferably, a method for constructing a cell model comprises: S1: The cells were cultured with F12 complete medium.

[0019] S2: Remove the supernatant from the cells in the growth phase in S1, wash with PBS, digest with trypsin, add F12 complete culture medium to obtain a cell suspension, and centrifuge to obtain cell 1.

[0020] S3: Subculture: Cell 1 is mixed with F12 complete medium and cultured at a constant temperature to obtain cell 2.

[0021] S4: Cell 2 is subcultured according to the proportion, a cell regeneration agent is added, and the cells are cultured at a constant temperature. When the culture is finished, the cells are washed with PBS to obtain a cell model.

[0022] More preferably, the cells are stem cells, including any one of hematopoietic stem cells, adipose stem cells, umbilical cord blood stem cells, and umbilical cord mesenchymal stem cells.

[0023] More preferably, the stem cells are umbilical cord mesenchymal stem cells.

[0024] More preferably, the culture time in S1 is 10 to 14 hours.

[0025] More preferably, the amount of PBS used for washing cells in S2 is 4 to 6 parts by volume.

[0026] More preferably, the mass percentage of pancreatic enzyme in S2 is 0.2-0.3%, the amount of pancreatic enzyme used is 0.5-1.5 parts by volume, and the digestion time is 1-3 min.

[0027] More preferably, the amount of F12 complete medium in S2 is 3 to 5 parts by volume.

[0028] More preferably, the centrifugal speed in S2 is 1000-2000 r / min, and the centrifugal time is 4-6 min.

[0029] More preferably, the amount of F12 complete medium in S3 is 8 to 12 parts by volume.

[0030] More preferably, the constant temperature culture temperature in S3 is 35-38°C.

[0031] More preferably, the ratio in S4 is 1:0.5~1.5.

[0032] More preferably, the amount of the cell regeneration agent in S4 is 0.2-0.8 parts by volume.

[0033] More preferably, the cell regeneration agent in S4 further comprises F12 complete medium, and the concentration of the composition in the F12 complete medium is 0.002-0.008 g / ml.

[0034] More preferably, the culture time in S4 is 15 to 25 hours.

[0035] More preferably, the constant temperature culture temperature in S4 is 35-39°C.

[0036] More preferably, the amount of PBS used for washing cells in S4 is 4 to 6 parts by volume, and the number of washes is 2 to 4 times.

[0037] The present invention also provides use of the composition in preparing a cell model and / or a wound repair material and / or a medicine and / or a wound recovery agent.

[0038] The present invention also provides the use of the cell regeneration agent in preparing a cell model and / or a wound repair material and / or a medicine and / or a wound recovery agent.

[0039] The present invention adopts the method of adding the composition to the F12 complete culture medium to prepare the cell regeneration agent, and uses it in the field of skin repair, so it has the following beneficial effects: the use of the composition not only effectively improves the regeneration ability of cells, but also has simple operation, low cost, and can be widely used in the field of skin repair. Therefore, the present invention is a composition for promoting cell regeneration and its application. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] Figure 1 This is the result of measuring absorbance using an enzyme-labeled instrument.

[0041] Figure 2 This is a graph showing the wound recovery rate in rats.

[0042] Figure 3 This is a graph showing the expression of CD63 in umbilical cord mesenchymal stem cells. DETAILED DESCRIPTION

[0043] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0044] The following first describes the concepts involved in the present application in conjunction with the accompanying drawings. It should be noted that the following description of each concept is only to make the content of the present application easier to understand, and does not limit the scope of protection of the present application; at the same time, the embodiments and features in the embodiments of the present application can be combined with each other in the absence of conflict. The present application will be described in detail with reference to the accompanying drawings and in conjunction with the embodiments.

[0045] Embodiment 1: A composition for promoting cell regeneration comprises: L-tryptophanol oxalate and isosorbide-2-acetate, wherein the mass ratio of L-tryptophanol oxalate to isosorbide-2-acetate is 1:1.

[0046] Embodiment 2: The difference between this embodiment and embodiment 1 is that the mass ratio of L-tryptophanol oxalate to isosorbide-2-acetate is 1:4.

[0047] Embodiment 3: The difference between this embodiment and embodiment 2 is that glabridol is added during the preparation of the composition, specifically: A composition for promoting cell regeneration comprises: L-tryptophanol oxalate, isosorbide-2-acetate and glabriquitol. The mass ratio of L-tryptophanol oxalate to isosorbide-2-acetate and glabriquitol is 1:4:1.

[0048] Embodiment 4: The difference between this embodiment and embodiment 3 is that the mass ratio of L-tryptophanol oxalate, isosorbide-2-acetate and glabriquitolan is 1:4:4.

[0049] Embodiment 5: A cell regeneration agent comprises: the composition prepared in Example 1 and F12 complete medium. The amount of the composition is 0.05 g, and the amount of the F12 complete medium is 10 ml.

[0050] Embodiment 6: A cell regeneration agent. The difference between this embodiment and embodiment 5 is that the composition is the composition of embodiment 2.

[0051] Embodiment 7: A cell regeneration agent. The difference between this embodiment and embodiment 5 is that the composition is the composition of embodiment 3.

[0052] Embodiment 8: A cell regeneration agent. The difference between this embodiment and embodiment 5 is that the composition is the composition of embodiment 4.

[0053] Comparative Example 1: A cell regeneration agent comprises: L-tryptophanol oxalate and F12 complete culture medium. The amount of L-tryptophanol oxalate is 0.05 g, and the amount of F12 complete culture medium is 10 ml.

[0054] Comparative Example 2: A cell regeneration agent comprises: isosorbide-2-acetate and F12 complete medium. The dosage of isosorbide-2-acetate is 0.05 g, and the dosage of F12 complete medium is 10 ml.

[0055] The cell counting method was used to detect the proliferation rate of mouse fibroblasts (L-929 cells) prepared by the cell regeneration agent in Example 5, so as to conduct a safety assessment on the toxicity of the cell regeneration agent. The results showed that the cell regeneration agent had a significant promoting effect on the proliferation of mouse fibroblasts (L-929 cells). Therefore, the cell regeneration agent prepared by the present invention is of practically non-toxic grade.

[0056] Embodiment 9: A method for constructing a cell model comprises the following steps: (1) Culture umbilical cord mesenchymal stem cells in F12 complete medium for 12 h.

[0057] (2) Remove the supernatant from the umbilical cord mesenchymal stem cells in the growth phase, aspirate PBS to wash the umbilical cord mesenchymal stem cells, add trypsin for digestion, invert and observe when the umbilical cord mesenchymal stem cells are in a free state, aspirate F12 complete medium and add it to the cell bottle, gently blow to make an umbilical cord mesenchymal stem cell suspension, and then centrifuge. The amount of PBS used to wash the umbilical cord mesenchymal stem cells is 5 mL, the mass percentage of trypsin used to digest the umbilical cord mesenchymal stem cells is 0.25%, the amount of trypsin is 1 mL, the digestion time is 2 min, the amount of F12 complete medium is 4 mL, the centrifugal speed is 1500 r / min, and the centrifugation time is 5 min.

[0058] (3) Add F12 complete medium at a ratio of 1 to 2, mix thoroughly by pipetting, and then culture in a constant temperature incubator. The amount of F12 complete medium used is 10 mL, and the constant temperature culture temperature is 37°C.

[0059] (4) At a ratio of 1:1, the umbilical cord mesenchymal stem cells were cultured in a cell bottle for a certain period of time and subcultured into a 6-well cell culture plate, and F12 complete medium was added to each cell well, and the cells were cultured in a constant temperature incubator. The umbilical cord mesenchymal stem cells were washed with PBS to obtain an umbilical cord mesenchymal stem cell model. The umbilical cord mesenchymal stem cells were cultured for 20 hours in the cell bottle, the amount of F12 complete medium was 0.5 mL, the constant temperature culture temperature was 37°C, the amount of PBS used to wash the umbilical cord mesenchymal stem cells was 5 mL, and the cells were washed with PBS 3 times.

[0060] Embodiment 10: A method for constructing a cell model.

[0061] The difference between this embodiment and embodiment 9 is that the F12 complete culture medium in step (4) of embodiment 9 is replaced with the cell regeneration agent in embodiment 5. Other than that, the other conditions are the same.

[0062] Embodiment 11: A method for constructing a cell model.

[0063] The difference between this embodiment and embodiment 9 is that the F12 complete culture medium in step (4) of embodiment 9 is replaced with the cell regeneration agent in embodiment 6. Other than that, the other conditions are the same.

[0064] Embodiment 12: A method for constructing a cell model.

[0065] The difference between this embodiment and embodiment 9 is that the F12 complete culture medium in step (4) of embodiment 9 is replaced with the cell regeneration agent in embodiment 7. Other than that, the other conditions are the same.

[0066] Embodiment 13: A method for constructing a cell model.

[0067] The difference between this embodiment and embodiment 9 is that the F12 complete culture medium in step (4) of embodiment 9 is replaced with the cell regeneration agent in embodiment 8. Other than that, the other conditions are the same.

[0068] Comparative Example 3: A method for constructing a cell model.

[0069] The difference between this embodiment and embodiment 9 is that the F12 complete culture medium in step (4) of embodiment 9 is replaced with the cell regeneration agent in comparative example 1. Other than that, the other conditions are the same.

[0070] Comparative Example 4: A method for constructing a cell model.

[0071] The difference between this embodiment and embodiment 9 is that the F12 complete culture medium in step (4) of embodiment 9 is replaced with the cell regeneration agent in comparative example 2. Other than that, the other conditions are the same.

[0072] Experimental Example 1 Cells: Umbilical cord mesenchymal stem cells Experimental group: umbilical cord mesenchymal stem cells cultured in Examples 9-13 Control group: umbilical cord mesenchymal stem cells cultured in comparative example 3-4 Experimental instrument: ELISA reader The experimental process and specific methods are as follows: Umbilical cord mesenchymal stem cells that have been continuously cultured to the fourth generation were selected, and a certain number of umbilical cord mesenchymal stem cells / well were inoculated into the well plate, and F12 complete medium was added. The F12 complete medium was evenly divided into 3 groups, which were recorded as A, B, and C groups. The 3 groups of F12 complete medium were used to culture the umbilical cord mesenchymal stem cells in the incubator for a certain period of time. After the culture was completed, the proliferation of the umbilical cord mesenchymal stem cells was checked using the CCK8 kit. Before the determination, the F12 complete medium was replaced with serum-free DMEM medium, the serum-free DMEM medium and the CCK8 solution were mixed evenly, and the culture was continued in the incubator. The absorbance was measured with an enzyme marker. The results are shown in the figure ( Figure 1 ). The number of umbilical cord mesenchymal stem cells was 4000, group A was cultured in the incubator for 48 hours, group B was cultured in the incubator for 96 hours, and group C was cultured in the incubator for 144 hours. The amount of serum-free DMEM medium was 100 μL, the amount of CCK8 solution was 10 μL, the culture temperature was 37°C, the culture time was 2 hours, and the measurement wavelength was 450 nm.

[0073] from Figure 1It can be seen that at each time point, the data results of the three groups of Examples 9 to 11 are compared, and the data of Example 9 is relatively small, indicating that the addition of L-tryptophanol oxalate and isosorbide-2-acetate to the F12 complete medium can effectively promote the proliferation of umbilical cord mesenchymal stem cells, and further illustrate that the addition of L-tryptophanol oxalate and isosorbide-2-acetate to the F12 complete medium is beneficial to promoting cell regeneration; at the same time, the higher the dosage of L-tryptophanol oxalate and isosorbide-2-acetate, the more conducive to promoting cell regeneration. The data results of the three groups of Examples 11 to 13 are compared, and the data of the two groups of Examples 12 and 13 are relatively large, indicating that the addition of glabridinol to the F12 complete medium can effectively promote the proliferation of umbilical cord mesenchymal stem cells, and further illustrate that the addition of glabridinol to the F12 complete medium is beneficial to promoting cell regeneration; at the same time, the higher the dosage of glabridinol, the more conducive to promoting cell regeneration. Comparing the data results of Example 10 and Comparative Examples 3-4, the data of Example 10 is higher, indicating that adding L-tryptophanol oxalate or isosorbide-2-acetate alone to F12 complete culture medium is not conducive to promoting cell regeneration.

[0074] Experimental Example 2 Experimental group: Cell regeneration agent of Examples 5-8 Control group: Cell regeneration agent of comparative example 1-2 Blank group: natural growth Experimental instrument: constant pressure and constant temperature scald instrument Thirty-two healthy rats with a weight difference of no more than 20g were selected and randomly divided into eight groups. A constant pressure and constant temperature scald instrument was used to create a rat deep second-degree scald model, so that the scald site was a deep second-degree scald. After 24 hours of observation, the wound surface showed obvious inflammatory changes, such as purple-red congestion, edema, etc. The area and depth of scald in rats in each group were similar, and the wound area of ​​the rats was recorded.

[0075] After the animal model was established, each group was housed in a cage. The wounds of the rats in the blank group were not treated. The wounds of the rats in the experimental and control groups were smeared with 0.5 ml of cell regeneration agent every day for 14 consecutive days. The growth of the wounds of the rats in each group was recorded, and the wound recovery rate of each group was calculated. The results are shown in Figure 2 shown.

[0076] The calculation formula of recovery rate is: Recovery rate = (S0-S t ) / S0, where S0 is the average initial area of ​​rat wounds and St is the average area of ​​rat wounds on day t.

[0077] from Figure 2It can be seen from the figure that the blank group is compared with the results of the three groups of Examples 5 to 6, and the wound recovery rates of the two groups of Examples 5 to 6 are significantly higher, indicating that the cell regeneration agent obtained by adding L-tryptophanol oxalate and isosorbide-2-acetate to the F12 complete medium is more conducive to the recovery of rat wounds, and further illustrates that adding L-tryptophanol oxalate and isosorbide-2-acetate to the F12 complete medium is beneficial to promoting cell regeneration; at the same time, the higher the amount of L-tryptophanol oxalate and isosorbide-2-acetate is used, the more conducive it is to promoting cell regeneration; the comparison of the results of the three groups of Examples 6 to 8, Example 7 ~8, the wound recovery rates of the two groups were significantly higher, indicating that the cell regeneration agent obtained by adding glabripenol to the F12 complete medium is more conducive to the recovery of rat wounds, which further shows that the addition of glabripenol to the F12 complete medium is beneficial to promoting cell regeneration; at the same time, the higher the amount of glabripenol, the more conducive to promoting cell regeneration; comparing the results of the blank group and the comparative examples 1~2, there was no significant improvement in the wound recovery rates of the two groups of comparative examples 1~2, indicating that the cell regeneration agent obtained by adding only L-tryptophanol oxalate or isosorbide-2-acetate to the F12 complete medium is not conducive to the recovery of rat wounds.

[0078] Experimental Example 3 Cells: Umbilical cord mesenchymal stem cells Experimental group: Cell models of Examples 9-13 Control group: cell model of comparative example 3-4 Exosomes are tiny vesicles secreted by cells. Exosomes mainly come from multivesicular bodies formed by the invagination of lysosomal microparticles in cells. Exosomes are composed of a lipid bilayer and contain all known molecular components of cells, mainly including: proteins, nucleic acids and cholesterol. Exosomes have many important functions in organisms, mainly including: cell communication and signal transmission, immune regulation, tissue repair and regeneration, drug delivery systems, and diagnosis and monitoring. Among them, the surface markers of exosomes are mainly CD63, CD81, CD9, TSG101 and HSP27. The amount of exosomes in the present invention is characterized by the detection result of CD63.

[0079] The expression of exosome marker protein CD63 was detected by Western Blot experiment. The test results are as follows Figure 3 shown.

[0080] from Figure 3It can be seen that, by comparing the results of the three groups of Examples 9 to 11, the CD63 expression levels of the two groups of Examples 10 and 11 are higher, indicating that adding L-tryptophanol oxalate and isosorbide-2-acetate to the F12 complete medium can effectively increase the expression level of CD63 in the cells, and further indicating that adding L-tryptophanol oxalate and isosorbide-2-acetate to the F12 complete medium can effectively increase the amount of exosomes in umbilical cord mesenchymal stem cells; by comparing the results of the three groups of Examples 11 to 13, the CD63 expression levels of the two groups of Examples 12 and 13 are higher, indicating that adding glabripenol to the F12 complete medium can effectively increase the amount of exosomes in umbilical cord mesenchymal stem cells. The expression level of CD63 in the umbilical cord mesenchymal stem cells was increased, indicating that adding L-tryptophanol oxalate and isosorbide-2-acetate to the F12 complete medium can effectively increase the amount of exosomes in the umbilical cord mesenchymal stem cells; comparing the three groups of data results of Example 9 and Comparative Examples 3~4, there was no obvious increase in the expression level of CD63 in the two groups of results of Comparative Examples 3~4, indicating that adding L-tryptophanol oxalate or isosorbide-2-acetate alone to the F12 complete medium is not conducive to increasing the expression level of CD63 in the umbilical cord mesenchymal stem cells, further indicating that adding L-tryptophanol oxalate or isosorbide-2-acetate alone to the F12 complete medium is not conducive to increasing the amount of exosomes in the umbilical cord mesenchymal stem cells.

[0081] The embodiments and / or implementation methods described above are only used to illustrate the preferred embodiments and / or implementation methods for realizing the technology of the present invention, and are not intended to limit the implementation methods of the technology of the present invention in any form. Any technical personnel in this field may make slight changes or modifications to other equivalent embodiments without departing from the scope of the technical means disclosed in the content of the present invention, but they should still be regarded as technologies or embodiments that are essentially the same as the present invention.

[0082] This article uses specific examples to illustrate the principles and implementation methods of this application. The description of the above embodiments is only used to help understand the method and its core ideas of this application. The above is only the preferred implementation method of this application. It should be pointed out that due to the limitations of textual expression and the objective existence of infinite specific structures, ordinary technicians in this technical field can make several improvements, modifications or changes without departing from the principles of this application, and can also combine the above technical features in an appropriate manner; these improvements, modifications, changes or combinations, or the direct application of the concept and technical solution of the invention to other occasions without improvement, should be regarded as the scope of protection of this application.

Claims

1. A composition comprising: At least one of L-tryptophanol oxalate and isosorbide-2-acetate.

2. A composition according to claim 1, characterized in that The L-tryptophanol oxalate and isosorbide-2-acetate are mixed in a mass ratio of 1:1-4.

3. A cell regeneration agent comprising: The composition according to any one of claims 1 to 2.

4. A cell regeneration agent according to claim 3, characterized in that: The cell regeneration agent also includes F12 complete culture medium, and the concentration of the composition in the F12 complete culture medium is 0.002-0.008 g / ml.

5. A method for constructing a cell model, comprising: The cell regeneration agent according to any one of claims 3-4 is used to culture cells to obtain a cell model.

6. The method for constructing a cell model according to claim 5, characterized in that: The cells include any one of hematopoietic stem cells, adipose stem cells, umbilical cord blood stem cells, and umbilical cord mesenchymal stem cells.

7. The method for constructing a cell model according to claim 5, characterized in that: The exosomes secreted by the cells in the cell model contain at least one of nucleic acid, protein and cholesterol.

8. The method for constructing a cell model according to claim 7, characterized in that: The surface markers of the exosomes include at least one of CD63, CD81, CD9, TSG101 and HSP27.

9. Use of the composition of claim 1 in preparing a cell model and / or a wound repair material and / or a medicine and / or a wound recovery agent.

10. Use of the cell regeneration agent according to claim 3 in preparing a cell model and / or a wound repair material and / or a medicine and / or a wound recovery agent.

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