Antibody chip and kit for detecting periodontitis related protein

Abstract

The invention discloses an antibody chip and a kit for detecting periodontitis-related protein in the technical field of biological detection, the antibody chip comprises a detection system and a detection substrate, the surface of the detection substrate is provided with a plurality of special-shaped holes, and the special-shaped holes are filled with specific antibodies of periodontitis-related protein. The kit comprises a handle, a rotating rod is arranged at the bottom of the handle, an upper fixing block and a lower fixing block are arranged on the side wall of the rotating rod, and a main gear is arranged on the lower portion of the rotating rod; a plurality of rotating shafts are arranged at the bottom of the upper fixing block; the bottoms of the rotating shafts are rotationally connected with the top of the lower fixing block; placing assemblies are arranged on the side walls of the rotating shafts, sector gears are fixedly connected to the lower portions of the rotating shafts, and the sector gears are meshed with the main gear. The main gear drives the rotating frame fixedly connected with the sector gear to rotate, the rotating frame is stored, and then a detection agent is stored; and by designing the special-shaped hole and adopting a micro-fluidic technology, the accuracy of a detection result is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of biological detection, and in particular relates to an antibody chip and a kit for detecting periodontitis-related proteins. Background Art

[0002] Periodontal disease is an inflammatory disease caused by multiple factors, which eventually leads to alveolar bone resorption, loss of periodontal attachment, deepening of periodontal pockets, etc. It is characterized by high prevalence, long duration of illness, and great harm. The prevalence and severity of periodontal disease increase with age. With the progressive development of inflammation, the supporting tissues around the teeth are progressively destroyed, resulting in the eventual loss of teeth.

[0003] Existing antibody chips for detecting periodontitis-related proteins mostly adopt a design in which the detection units are completely isolated from each other. However, this design may result in excessive input of samples, leading to serious waste of samples.

[0004] In summary, some existing antibody chips for detecting periodontitis-related proteins may have excessive samples during sample testing, resulting in serious waste of samples, which has become a difficult problem that needs to be solved urgently in this field. Therefore, it is necessary to propose an antibody chip and kit for detecting periodontitis-related proteins. Summary of the invention

[0005] In order to solve the above problems, the purpose of the present invention is to propose an antibody chip and a kit for detecting periodontitis-related proteins, in which a rotating frame fixedly connected to a fan-shaped gear is driven by a main gear to rotate, thereby storing the rotating frame and further storing the detection agent; by designing special-shaped holes and adopting microfluidic technology, the accuracy of the detection results is improved.

[0006] In order to achieve the above-mentioned purpose, the technical scheme of the present invention is as follows: a kit for detecting periodontitis-related proteins, comprising an antibody chip and a kit; the kit comprises a handle, a rotating rod is fixedly connected to the bottom of the handle, and the side walls of the rotating rod are rotated from top to bottom in sequence to cooperate with an upper fixed block and a lower fixed block; a main gear is fixedly connected to the lower part of the rotating rod, and the main gear is located between the upper fixed block and the lower fixed block; a plurality of rotating shafts are rotatably connected to the bottom of the upper fixed block, and the bottoms of the rotating shafts are rotatably connected to the top of the lower fixed block; the side walls of the rotating shafts are provided with placement components for placing antibody chips, and the lower parts of the rotating shafts are fixedly connected to fan-shaped gears, which are meshed with the main gears.

[0007] The following principles and beneficial effects are achieved by adopting the above scheme:

[0008] Basic principle: The staff turns the handle. Since the lower part of the rotating rod fixedly connected to the bottom of the handle is fixedly connected to the main gear, when the handle is turned, the main gear will also rotate with the handle. At this time, since the fan gears are all engaged with the main gear, when the main gear rotates, the fan gears will also rotate driven by the main gear, and all the placement components will be unscrewed for the staff to perform periodontitis-related protein detection operations.

[0009] The upper fixed block and the lower fixed block will remain relatively still when the staff turns the handle. At this time, since the rotating shaft is in rotational cooperation with the upper fixed block and the lower fixed block, the rotating shaft has little effect on the reagent kit during rotation, thereby ensuring the stability of the reagent kit.

[0010] Beneficial effects: 1. The present invention realizes the storage of the placement components and further the storage of the detection agent by driving the rotation of the rotating frame fixedly connected to the fan gear through the main gear, which greatly reduces the possibility of the detection agent being damaged during the storage process, thereby causing serious waste of the detection agent.

[0011] 2. The design of the rotating shaft in conjunction with the gear transmission mechanism in the present invention enables high-precision positioning and rotation control of the antibody chip, greatly improving the accuracy of the test results. At the same time, the staff can perform batch tests on samples by rotating the rotating frame during subsequent tests, greatly improving the test efficiency.

[0012] 3. The design of the placement component being arranged on the side wall of the rotating shaft in the present invention enables the placement component to be rotated together with the rotating shaft when the sector gear is driven to rotate, thereby making the storage of the placement component more convenient.

[0013] Furthermore, the placement component includes a rotating frame fixedly connected to the side wall of the rotating shaft, the inner wall of the rotating frame is fixedly connected with a partition, the partition divides the interior of the rotating frame into a chip cavity and a detection cavity, the antibody chip is placed in the chip cavity, the detection cavity is placed with a detection agent, the inner wall of the detection cavity is fixedly connected with a thermometer and a hygrometer, and the thermometer and a hygrometer are electrically connected to a controller; the partition is fixedly connected with an exhaust fan, and the controller is used to control the start of the exhaust fan.

[0014] Beneficial effects: The antibody chip is safely placed in the chip cavity and kept at a certain distance from the detection agent, which greatly reduces the possibility of contamination, corrosion or damage caused by direct contact between the antibody chip and the detection agent. The isolation effect of the partition on the antibody chip and the detection agent further ensures the safety of the antibody chip during the detection process. At the same time, the design of the rotating frame allows the size, shape and position of the chip cavity and the detection cavity to be adjusted according to actual needs to adapt to antibody chips of different sizes and types and detection needs.

[0015] Furthermore, a plurality of anti-slip grooves are provided on the side wall of the handle.

[0016] Beneficial effect: When the staff turns the handle, the design of the anti-slip groove increases the friction between the handle and the staff's hand, making it easier for the staff to rotate the placement component out.

[0017] Furthermore, a kit suitable for the above-mentioned detection of periodontitis-related proteins is an antibody chip for detecting periodontitis-related proteins, including a detection system and a detection substrate, wherein a plurality of irregular holes are opened on the surface of the detection substrate, adjacent irregular holes are interconnected, and the irregular holes are filled with specific antibodies for periodontitis-related proteins.

[0018] Beneficial effects: The design of the special-shaped holes makes the specific antibodies of periodontitis proteins adhere to a wider surface in the special-shaped holes, so that when the staff detect periodontitis-related proteins, the contact surface between the sample and the specific antibodies of periodontitis proteins is larger, thereby improving the detection sensitivity. The design of adjacent special-shaped holes being interconnected allows the sample to enter the adjacent special-shaped holes when there is an excess of sample, thereby reducing the waste of sample.

[0019] Furthermore, the detection system includes the following modules:

[0020] The chip manufacturing module is used to complete the manufacture of antibody chips for detecting periodontitis-related proteins.

[0021] The sample processing module is used to centrifuge, filter and dilute the sample.

[0022] The sample detection module is used to detect the sample through an antibody chip for detecting periodontitis-related proteins and obtain the detection results.

[0023] The data analysis module is used to analyze the test results obtained by the sample detection module and obtain the analysis results.

[0024] Beneficial effects: The chip manufacturing module first manufactures the antibody chip for detecting periodontitis-related proteins, and the sample processing module centrifuges, filters and dilutes the collected samples. After the antibody chip for detecting periodontitis-related proteins is manufactured and the sample processing is completed, the sample detection module detects the sample through the antibody chip for detecting periodontitis-related proteins and obtains the detection results. Finally, the data analysis module analyzes the detection results and obtains the analysis results.

[0025] Furthermore, when testing the sample, microfluidics technology is used to control the release amount of the sample.

[0026] Beneficial effects: Microfluidic technology can accurately control the amount of sample released, thereby reducing errors caused by excessive or insufficient sample volume when testing samples, thereby improving the accuracy and sensitivity of the test.

[0027] Further, the method for preparing the antibody chip is as follows:

[0028] Step 1: substrate pretreatment: use the solid phase carrier as the detection substrate, and disinfect, clean and polish the detection substrate. The solid phase carrier is a glass slide.

[0029] Step 2: Molecular fixation: Prepare specific antibodies against periodontitis-related proteins, and fix the specific antibodies against periodontitis-related proteins in the special-shaped holes by covalent binding.

[0030] Step three, micromachining: use photolithography technology to micromachining the detection substrate, and add additives, cross-linking agents and non-specific proteins into the special-shaped holes in sequence, wherein the additive is a silane coupling agent, the cross-linking agent is carbodiimide, and the non-specific protein is gelatin.

[0031] Step 4: Chip sealing: Use polymer material to seal the antibody chip, and the polymer material is polymethyl methacrylate.

[0032] Beneficial effects: The staff first took out the solid phase carrier as the detection substrate, and pre-treated it, disinfected, cleaned and polished it. After completing the pre-treatment of the detection substrate, the staff fixed the specific antibodies of periodontitis-related proteins in the special-shaped holes on the surface of the detection substrate, and then used photolithography technology to micro-process the detection substrate to further form a microstructure on the surface of the detection substrate, and added additives, cross-linking agents and non-specific proteins in the special-shaped holes in turn, so that the specific antibodies of periodontitis-related proteins are closely combined with the detection substrate, and the possibility of contamination of the specific antibodies of periodontitis-related proteins is reduced. Finally, the staff used polymer materials to seal the antibody chip to complete the preparation of the antibody chip.

[0033] The surface of the glass slide is usually chemically inert and can resist corrosion from a variety of chemical reagents, thereby ensuring that no unnecessary chemical reactions occur with samples or reagents during the detection or analysis process.

[0034] Covalent bonding is a powerful chemical bonding method that can ensure that antibody molecules are firmly fixed to the surface of the detection substrate and are not easily detached or denatured.

[0035] Silane coupling agents can establish effective chemical bonds between inorganic materials and organic materials, significantly improving the bonding strength between these two materials of different properties.

[0036] Carbodiimide crosslinkers can react chemically with polymer materials to form a three-dimensional crosslinked structure, which can firmly bind the bonds between molecules together, further enhancing the binding force between the antibody molecules and the solid phase carrier.

[0037] Gelatin has good sealing properties and can effectively seal the sites on the solid phase carrier that are not occupied by periodontitis-related proteins, thereby reducing the adsorption and binding of nonspecific proteins in subsequent operations and reducing the occurrence of nonspecific reactions.

[0038] Using polymethyl methacrylate to seal the antibody chip can effectively isolate the impact of the external environment on the inside of the antibody chip, thereby keeping the internal environment of the antibody chip clean and stable and ensuring the accuracy of the test results.

[0039] Furthermore, in step three, gold nanoparticles and avidin are added into the special-shaped holes when micro-machining the detection substrate.

[0040] Beneficial effects: Gold nanoparticles have good biocompatibility. By adding gold nanoparticles to the shaped holes, the biological activity of the detection substrate can be significantly improved. Avidin is a protein that can specifically bind to biotin with high affinity and specificity. After combining avidin with gold nanoparticles, the detection substrate can be endowed with the ability to recognize and bind to specific biomolecules, such as periodontitis-related proteins, thereby achieving specific capture and detection of periodontitis-related proteins.

[0041] Furthermore, the steps for preparing specific antibodies against periodontitis-related proteins are as follows:

[0042] S1, preparing antigens: culturing periodontitis-related proteins in a culture medium for 2 to 3 days, collecting the periodontitis-related proteins after the culturing, and crushing them by ultrasonic crushing, adding purified water and salt solution to extract the periodontitis-related proteins, and finally purifying the periodontitis-related proteins by centrifugation to obtain antigens;

[0043] S2, immunization: the antigen is mixed with Freund's adjuvant and injected subcutaneously into healthy mammals;

[0044] S3, antibody collection: After the immunization of the mammal is completed, the serum of the mammal is collected and impurities in the serum are removed to obtain serum containing specific antibodies, and the serum is purified using antibody purification technology to obtain antibodies.

[0045] Beneficial effects: The staff first cultured, extracted and purified periodontitis-related proteins, mixed the purified antigens with Freund's adjuvant, and injected them into healthy mammals by subcutaneous injection. After the mammals were immunized, the staff collected the serum of the mammals, centrifuged the serum to remove impurities, and finally used antibody purification technology on the serum of the mammals to obtain antibodies.

[0046] Furthermore, in S1, the periodontitis-related protein is selected from one or more of the pilus proteins on the surface of Porphyromonas gingivalis, the outer membrane proteins of Porphyromonas gingivalis, or the salivary proteins of the patient.

[0047] Beneficial effects: The pilus proteins on the surface of Porphyromonas gingivalis have strong immunogenicity and can effectively stimulate the host to produce an immune response, thereby producing high-quality antibodies. The outer membrane proteins of Porphyromonas gingivalis are also closely related to its pathogenicity. BRIEF DESCRIPTION OF THE DRAWINGS

[0048] Figure 1 Schematic diagram of the axonometric view of the reagent kit when unfolded in an embodiment of the present invention.

[0049] Figure 2 Schematic diagram of a top view of the reagent box when it is closed in an embodiment of the present invention.

[0050] Figure 3 It is a top view schematic cross-sectional view of the reagent box when it is unfolded in an embodiment of the present invention.

[0051] Figure 4 FIG. 4 is a flow chart of the preparation of the antibody chip in the embodiment of the present invention.

[0052] Figure 5 Flow chart of the preparation of specific antibodies against periodontitis-related proteins in the embodiments of the present invention.

[0053] Figure 6 It is a front cross-sectional view of the antibody chip in an embodiment of the present invention.

[0054] Figure 7 Schematic diagram of the structure of the detection system in the embodiment of the present invention.

[0055] The reference numerals in the drawings of the specification include: 1, handle; 2, rotating rod; 3, upper fixed block; 4, lower fixed block; 5, main gear; 6, rotating shaft; 7, sector gear; 8, rotating frame; 9, detection base; 10, special-shaped hole. DETAILED DESCRIPTION

[0056] The following is further described in detail through specific implementation methods:

[0057] Embodiment 1:

[0058] Basically as attached Figure 1 , Figure 2 and Figure 3 As shown:

[0059] A kit for detecting periodontitis-related proteins, comprising an antibody chip and a kit; the kit comprises a handle 1, with a plurality of anti-slip grooves on the side wall of the handle 1; a rotating rod 2 is welded at the bottom of the handle 1, and the side walls of the rotating rod 2 are rotated from top to bottom in sequence with an upper fixed block 3 and a lower fixed block 4; a main gear 5 is coaxially fixedly welded at the lower part of the rotating rod 2, and the main gear 5 is located between the upper fixed block 3 and the lower fixed block 4; a plurality of rotating shafts 6 are rotatably connected to the bottom of the upper fixed block 3, and the bottoms of the rotating shafts 6 are all rotatably connected to the top of the lower fixed block 4.

[0060] The side walls of the rotating shaft 6 are provided with placement components for placing antibody chips; the placement components include a rotating frame 8 welded to the side walls of the rotating shaft 6, and the inner walls of the rotating frame 8 are welded with partitions, which divide the interior of the rotating frame 8 into a chip cavity and a detection cavity, the antibody chip is placed in the chip cavity, and the detection cavity is placed with a detection agent.

[0061] The inner wall of the detection chamber is fixedly connected with a thermometer and hygrometer by bolts, and the thermometer and hygrometer is electrically connected with a controller; the partition is fixedly connected with an exhaust fan, and the controller is used to control the start of the exhaust fan; the lower part of the rotating shaft 6 is welded with a fan gear 7, and the fan gear 7 is meshed with the main gear 5.

[0062] The specific implementation process is as follows: the staff rotates the handle 1, driving the rotating rod 2 welded to the bottom of the handle 1 to rotate, and then driving the main gear 5 welded to the side wall of the rotating rod 2 to rotate.

[0063] like Figure 3 As shown, since the sector gears 7 are all meshed with the main gear 5, when the main gear 5 rotates, the sector gear 7 will also be driven by the main gear 5, and then the sector gear 7 will drive the rotating shaft 6 welded thereto to rotate. Since the rotating frame 8 is welded to the side wall of the rotating shaft 6, when the rotating shaft 6 rotates, the rotating frame 8 will rotate together with the rotating shaft 6, and then all the rotating frames 8 are rotated out. At this time, when the staff is performing the detection of periodontitis-related proteins, the positioning and rotation of the antibody chip can be achieved through simple manual operation, thereby facilitating batch detection and greatly improving the detection efficiency. In this embodiment, the detection agent placed in the detection chamber contains a protease inhibitor.

[0064] During the detection process, the thermometer and hygrometer will monitor the current ambient temperature and humidity in real time, and send the monitored temperature and humidity signals to the controller. When an abnormal situation occurs, the controller will send the corresponding alarm information to the terminal device of the staff. At this time, the staff can judge the temperature and humidity of the environment through the terminal device, and then adjust the ambient temperature and humidity to ensure the accuracy of the test results. For example, when the ambient humidity is 35% and the ambient temperature is 30°C, after receiving the temperature and humidity signal, the controller determines that the ambient humidity is normal at this time, but the ambient temperature is high, which is an abnormal state. At this time, the controller controls the exhaust fan to start, cool down the antibody chip cavity and the detection cavity, and send an alarm message of high ambient temperature to the terminal device of the staff so that the staff can take corresponding preventive measures.

[0065] Embodiment 2:

[0066] Basically as attached Figure 6 and Figure 7 As shown:

[0067] The difference from the above-mentioned embodiment is that the kit for detecting periodontitis-related proteins is suitable for the above-mentioned periodontitis-related protein detection, an antibody chip for detecting periodontitis-related proteins, including a detection system and a detection substrate 9, a plurality of special-shaped holes 10 are opened on the surface of the detection substrate 9, adjacent special-shaped holes 10 are interconnected, and the special-shaped holes 10 are filled with specific antibodies for periodontitis-related proteins.

[0068] The detection system includes the following modules:

[0069] The chip manufacturing module is used to complete the manufacture of antibody chips for detecting periodontitis-related proteins.

[0070] The sample processing module is used to centrifuge, filter and dilute the sample.

[0071] The sample detection module is used to control the release amount of the sample by using microfluidic technology, and to detect the sample through an antibody chip for detecting periodontitis-related proteins and obtain the test results.

[0072] The data analysis module is used to analyze the test results obtained by the sample detection module and obtain the analysis results.

[0073] The specific implementation process is as follows: the chip manufacturing module first manufactures the antibody chip, while the sample processing module centrifuges, filters and dilutes the sample. After the chip manufacturing module completes the manufacture of the antibody chip and the sample processing module completes the sample processing, the sample detection module takes out the sample and the antibody chip. At this time, the sample detection module sends the sample to the antibody chip through microfluidics technology, detects the sample, and obtains the test result.

[0074] After the sample detection module obtains the detection result, the data analysis module will analyze the detection result of the sample detection module to obtain the analysis result.

[0075] Embodiment 3:

[0076] Basically as attached Figure 4 and Figure 6 As shown:

[0077] The difference from the above embodiment is that the method for making the antibody chip is as follows:

[0078] Step 1: substrate pretreatment: use the solid phase carrier as the detection substrate 9, and sterilize, clean and polish the detection substrate 9. The solid phase carrier is a glass sheet.

[0079] Step 2: Molecular fixation: Prepare specific antibodies of periodontitis-related proteins, and fix the specific antibodies of periodontitis-related proteins in the special-shaped holes 10 by covalent binding.

[0080] Step three, micromachining: Use photolithography technology to micromachining the detection substrate 9, and add silane coupling agent as an additive, carbodiimide as a cross-linking agent and gelatin as a non-specific protein into the shaped hole 10 in sequence, and add gold nanoparticles and anti-biotin protein into the shaped hole 10 at the same time.

[0081] Step 4: Chip sealing: Use polymer materials to seal the antibody chip, wherein the polymer material is polymethyl methacrylate.

[0082] The specific implementation process is as follows: the staff first takes out the glass sheet as the detection substrate 9, and disinfects, cleans and polishes it to complete the pretreatment of the detection substrate 9. After completing the pretreatment of the detection substrate 9, the staff uses covalent bonding to fix the specific antibodies of the periodontitis-related proteins in the special-shaped holes 10, so that the antibody molecules are firmly fixed in the special-shaped holes 10 and are not easy to fall off or denature. Subsequently, the staff uses photolithography technology to micro-process the detection substrate 9, so that the microstructure is further formed on the surface of the detection substrate 9, and gold nanoparticles, avidin, silane coupling agent, carbodiimide and gelatin are added to the special-shaped holes 10 in sequence. At this time, the binding force between the antibody molecules and the detection substrate 9 is enhanced, and the antibody molecules will form a three-dimensional cross-linked structure with the detection substrate 9, thereby further enhancing the binding force between the antibody molecules and the detection substrate 9. The use of gold nanoparticles and avidin improves the biological activity of the detection substrate 9, and at the same time, the specificity of the detection substrate 9 is also improved. After gelatin is added, the sites on the detection substrate 9 that are not occupied by periodontitis-related proteins will be blocked, thereby reducing the interference of non-specific proteins on the detection results and making the detection results more reliable.

[0083] After the micro-processing is completed, the staff uses polymethyl methacrylate to seal the antibody chip to reduce the impact of the external environment on the inside of the antibody chip and complete the preparation of the antibody chip.

[0084] Embodiment 4:

[0085] Basically as attached Figure 5 As shown:

[0086] The difference from the above embodiment is that the preparation steps of the specific antibody of periodontitis-related protein are as follows:

[0087] S1, preparing antigens: culturing periodontitis-related proteins in a culture medium for 2 to 3 days, in this embodiment, the culturing time is 3 days, collecting the periodontitis-related proteins after the culturing, and crushing them by ultrasonic crushing, adding purified water and salt solution to extract the periodontitis-related proteins, and finally purifying the periodontitis-related proteins by centrifugation to obtain antigens; the periodontitis-related proteins are selected from one or more of the pilus proteins on the surface of Porphyromonas gingivalis, the outer membrane proteins of Porphyromonas gingivalis, or the salivary proteins of patients, and in this embodiment, the pilus proteins are selected;

[0088] S2, immunization: the antigen is mixed with Freund's adjuvant and injected into a healthy mammal by subcutaneous injection. In this embodiment, the mammal is a mouse;

[0089] S3, antibody collection: After the immunization of the mammal is completed, the serum of the mammal is collected and impurities in the serum are removed to obtain serum containing specific antibodies, and the serum is purified using antibody purification technology to obtain antibodies.

[0090] The specific implementation process is as follows: the staff first cultured the pilus protein in a culture medium for 3 days. After the culture was completed, the pilus protein was collected and crushed by ultrasonic crushing, and purified water and saline solution were added to extract the pilus protein. Finally, the extracted periodontitis-related protein was centrifuged in a centrifuge to complete the purification operation and obtain the antigen. The antigen was mixed with Freund's adjuvant and injected into the mouse by subcutaneous injection. After the mouse immunization was completed, the staff collected the serum of the mouse and removed impurities from the serum of the mouse. In this embodiment, the staff used a pipette to put the serum of the mouse into a centrifuge tube, and the centrifuge tube was placed in a centrifuge for centrifugation to remove impurities in the serum.

[0091] After the impurities in the serum are removed, the staff uses antibody purification technology to purify the serum to produce antibodies.

[0092] The above is only an embodiment of the present invention. The common sense such as the known specific structure and characteristics in the scheme is not described in detail here. The ordinary technicians in the relevant field know all the common technical knowledge in the technical field of the invention before the application date or priority date, can know all the existing technologies in the field, and have the ability to apply the conventional experimental means before that date. The ordinary technicians in the relevant field can improve and implement this scheme in combination with their own abilities under the enlightenment given by this application. Some typical known structures or known methods should not become obstacles for ordinary technicians in the relevant field to implement this application. It should be pointed out that for those skilled in the art, without departing from the structure of the present invention, several deformations and improvements can be made, which should also be regarded as the protection scope of the present invention, which will not affect the effect of the implementation of the present invention and the practicality of the patent. The protection scope required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. A kit for detecting periodontitis-related proteins, characterized in that: The kit comprises an antibody chip and a test kit; the test kit comprises a handle (1), the bottom of the handle (1) is fixedly connected to a rotating rod (2), the side wall of the rotating rod (2) is rotatably matched with an upper fixed block (3) and a lower fixed block (4) from top to bottom; the lower part of the rotating rod (2) is coaxially fixedly connected to a main gear (5), and the main gear (5) is located between the upper fixed block (3) and the lower fixed block (4); the bottom of the upper fixed block (3) is rotatably connected to a plurality of rotating shafts (6), and the bottoms of the rotating shafts (6) are all rotatably connected to the top of the lower fixed block (4); The side walls of the rotating shaft (6) are provided with a placement assembly for placing the antibody chip, and the lower part of the rotating shaft (6) is fixedly connected with a sector gear (7), and the sector gear (7) is meshed with the main gear (5).

2. The kit for detecting periodontitis-related proteins according to claim 1, characterized in that: The placement component comprises a rotating frame (8) fixedly connected to the side wall of the rotating shaft (6); the inner wall of the rotating frame (8) is fixedly connected with a partition, the partition divides the interior of the rotating frame (8) into a chip cavity and a detection cavity, the antibody chip is placed in the chip cavity, the detection cavity is placed with a detection agent, the inner wall of the detection cavity is fixedly connected with a thermometer and hygrometer, and the thermometer and hygrometer are electrically connected to a controller; the partition is fixedly connected with an exhaust fan, and the controller is used to control the start of the exhaust fan.

3. The kit for detecting periodontitis-related proteins according to claim 2, characterized in that: A plurality of anti-slip grooves are provided on the side wall of the handle (1).

4. An antibody chip for detecting periodontitis-related proteins, suitable for the kit for detecting periodontitis-related proteins according to any one of claims 1 to 3, characterized in that: The detection system comprises a detection system and a detection substrate (9). The detection substrate (9) has a plurality of irregularly shaped holes (10) on its surface. Adjacent irregularly shaped holes (10) are interconnected. The irregularly shaped holes (10) are filled with specific antibodies to periodontitis-related proteins.

5. The antibody chip for detecting periodontitis-related proteins according to claim 4, characterized in that: The detection system includes the following modules: A chip manufacturing module is used to complete the manufacture of antibody chips for detecting periodontitis-related proteins; A sample processing module, used for centrifuging, filtering and diluting the sample; A sample detection module is used to detect the sample through an antibody chip for detecting periodontitis-related proteins and obtain the detection result; The data analysis module is used to analyze the test results obtained by the sample detection module and obtain the analysis results.

6. The antibody chip for detecting periodontitis-related proteins according to claim 5, characterized in that: When testing samples, microfluidics technology is used to control the release amount of samples.

7. The antibody chip for detecting periodontitis-related proteins according to claim 6, characterized in that: The method for making the antibody chip is as follows: Step 1, substrate pretreatment: using a solid phase carrier as a detection substrate (9), and sterilizing, cleaning and polishing the detection substrate (9), wherein a glass sheet is selected as the solid phase carrier; Step 2, molecular fixation: preparing specific antibodies for periodontitis-related proteins, and fixing the specific antibodies for periodontitis-related proteins in the special-shaped holes (10), wherein the fixation method is covalent bonding; Step 3, micro-machining: micro-machining the detection substrate (9) using photolithography technology, and sequentially adding additives, cross-linking agents and non-specific proteins into the special-shaped holes (10), wherein the additive is a silane coupling agent, the cross-linking agent is carbodiimide, and the non-specific protein is gelatin; Step 4: Chip sealing: Use polymer material to seal the antibody chip, and the polymer material is polymethyl methacrylate.

8. The antibody chip for detecting periodontitis-related proteins according to claim 7, characterized in that: In step three, gold nanoparticles and avidin are added into the irregularly shaped holes (10) when micro-machining the detection substrate (9).

9. The antibody chip for detecting periodontitis-related proteins according to claim 8, characterized in that: The steps for producing specific antibodies against periodontitis-related proteins are as follows: S1, preparing antigens: culturing periodontitis-related proteins in a culture medium for 2 to 3 days, collecting the periodontitis-related proteins after the culturing, and crushing them by ultrasonic crushing, adding purified water and salt solution to extract the periodontitis-related proteins, and finally purifying the periodontitis-related proteins by centrifugation to obtain antigens; S2, immunization: the antigen is mixed with Freund's adjuvant and injected subcutaneously into healthy mammals; S3, antibody collection: After the immunization of the mammal is completed, the serum of the mammal is collected and impurities in the serum are removed to obtain serum containing specific antibodies, and the serum is purified using antibody purification technology to obtain antibodies.

10. The antibody chip for detecting periodontitis-related proteins according to claim 9, characterized in that: In S1, the periodontitis-related proteins are selected from one or more of the fimbriae proteins on the surface of Porphyromonas gingivalis, the outer membrane proteins of Porphyromonas gingivalis, or the salivary proteins of the patient.

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