Skin barrier multi-repairing composition, face cream, preparation method and application

Abstract

The invention discloses a skin barrier multi-repairing composition, face cream, a preparation method and application, and relates to the technical field of skin care product processing. The skin barrier multi-repair composition is prepared from mannitol, trehalose, fibronectin, phosphatidylcholine, ginsenoside, polysorbate-80, dipropylene glycol, p-hydroxyacetophenone, 1, 2-hexanediol, caprylyl glycol, isoprene glycol and water. Meanwhile, face cream can be prepared by adding the multi-repairing composition and face cream components. Experiments prove that the multi-repairing composition has a repairing effect, the face cream is suitable for sensitive skin groups, has the characteristics of moisturizing, repairing, relieving, mildness and no irritation, and is good in absorption effect, the preparation method is simple, large equipment is not needed, and the production period is short.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care product processing, and in particular to a skin barrier multiple repairing composition, a face cream, a preparation method and an application thereof. Background Art

[0002] Sensitive skin refers to a highly reactive state of the skin under physiological or pathological conditions. Due to problems with the skin's natural defense system, it loses its barrier ability to external stimuli. It may manifest with subjective symptoms such as burning, stinging, itching and tightness. In severe cases, it is accompanied by objective characteristics such as erythema, capillary dilation and desquamation, mainly occurring on the face.

[0003] To care for this kind of sensitive skin, some non-irritating skin care products are needed. However, the existing non-irritating skin care products themselves have poor efficacy and cannot achieve the expected effect of repairing sensitive skin. There may be mutual inhibition of efficacy, resulting in poor effect of repairing sensitive skin and causing more serious consequences. Summary of the invention

[0004] In view of the above-mentioned defects of the prior art, the present invention proposes a skin barrier multi-repair composition, a facial cream, a preparation method and an application to solve the problems raised by the above-mentioned background technology.

[0005] In order to achieve the above object, the present invention provides the following technical solutions:

[0006] A skin barrier multi-repair composition comprises the following components in percentage by mass: 0.4-14.84% mannitol, 0.01-1.53% trehalose, 0.01-0.77% fibronectin, 0.42-7.32% phosphatidylcholine, 0.03-1.31% ginsenoside, 0.0001-0.1% polysorbate-80, 0.26-0.77% dipropylene glycol, 0.14-0.41% p-hydroxyacetophenone, 0.06-0.17% 1,2-hexanediol, 0.04-0.12% caprylyl glycol, 0.02-0.05% isoprene glycol, and the balance is water.

[0007] Preferably, a skin barrier multi-repair composition comprises the following components in percentage by mass: 7.62% mannitol, 0.76% trehalose, 0.38% fibronectin, 3.86% phosphatidylcholine, 0.67% ginsenoside, 0.001% polysorbate 80, 0.51% dipropylene glycol, 0.27% parahydroxyacetophenone, 0.11% 1,2-hexanediol, 0.08% caprylyl glycol, 0.03% isoprene glycol, and the balance is water.

[0008] Preferably, a method for preparing a skin barrier multi-repairing composition comprises the following steps:

[0009] (1) Fibronectin, mannitol, 1,2-hexanediol, dipropylene glycol, p-hydroxyacetophenone and water are mixed and stirred at a temperature of 25 to 40° C. to prepare a premix solution;

[0010] (2) mixing ginsenoside, isopentyl glycol, caprylyl glycol, trehalose, phosphatidylcholine and polysorbate 80 at a temperature of 30 to 40° C. to obtain a primary mixed solution;

[0011] (3) The initial mixed solution is added dropwise to the premixed solution at a rate of 0.8 to 1.5 mL / min, and stirred at 300 to 400 rpm for 30 to 50 min at a temperature of 40 to 50° C. to obtain a skin barrier multi-repairing composition.

[0012] Preferably, a facial cream comprises phase A, phase B, phase C and phase D, each phase being mixed with components in the following mass percentages, and the remainder being water:

[0013] The phase A comprises 0.01-0.05% of a macromolecular sodium hyaluronate with a molecular weight of 1 million to 1.8 million Da, 0.02-0.10% of a medium molecular sodium hyaluronate with a molecular weight of 200,000 to 400,000 Da, 0.04-0.20% of a small molecular sodium hyaluronate with a molecular weight of less than 10,000 Da, and 0.10-0.30% of carbomer;

[0014] The phase B comprises 0.01-5% skin barrier multi-repair composition, 0.10-1.0% dipotassium glycyrrhizinate, 1-4% betaine, 2-6% 1,2-pentanediol, 0.02-1% sphingomonas fermentation product extract, 2-7% glycerol, 0.5-2% 1,2-hexanediol, and 0.1-0.6% raspberry ketone;

[0015] The phase C comprises 0.2-0.8% cetearyl glucoside, 1-5% eicosyl glucoside, 0.1-0.5% behenyl alcohol, 1.05-5% polydimethylsiloxane, and 0.22-1.25% sodium polyacryloyl dimethyl taurate;

[0016] The phase D comprises 1-4% of damask rose flower water, 0.5-2% of p-hydroxyacetophenone, 0.5-2% of 1,2-hexanediol and 0.1-0.2% of aminomethyl propanol.

[0017] Preferably, a facial cream comprises phase A, phase B, phase C and phase D, each phase being mixed with components in the following mass percentages, and the remainder being water:

[0018] The phase A comprises 0.02% of macromolecular sodium hyaluronate with a molecular weight of 1 million to 1.8 million Da, 0.04% of medium molecular sodium hyaluronate with a molecular weight of 200,000 to 400,000 Da, 0.06% of small molecular sodium hyaluronate with a molecular weight of less than 10,000 Da, and 0.25% of carbomer;

[0019] The phase B includes 3% skin barrier multi-repair composition, 0.15% dipotassium glycyrrhizinate, 2% betaine, 3% 1,2-pentanediol, 0.04% sphingomonas fermentation product extract, 3.5% glycerol, 1% 1,2-hexanediol, and 0.4% raspberry ketone;

[0020] The phase C includes 0.5% cetearyl glucoside, 2.5% eicosyl glucoside, 0.3% behenyl alcohol, 2% dimethicone, and 0.5% sodium polyacryloyldimethyl taurate;

[0021] The phase D includes 3% damask rose flower water, 1% p-hydroxyacetophenone, 1% 1,2-hexanediol, and 0.15% aminomethyl propanol.

[0022] Preferably, a method for preparing a facial cream comprises the following steps:

[0023] (1) Add water to the phase A component and stir at 35-45 rpm for 15-25 min to swell and mix, then add the phase B component, heat to 75-85°C, and stir at 30-40 rpm until completely dissolved to obtain a phase AB mixture;

[0024] (2) Stir the C phase components at 25-35 rpm for 30-40 min at a temperature of 75-85°C to mix them evenly;

[0025] (3) Mix the AB phase mixture and the C phase components, homogenize at 2500-3000 rpm for 12-20 min, and then continue stirring at 25-35 rpm for 20-40 min to obtain an ABC phase mixture;

[0026] (4) Stir the ABC phase mixture at 25-35 rpm and cool it to 40-50°C, add the D phase component, continue stirring to 22-26°C, degas, filter through 150-200 mesh, and obtain the facial cream.

[0027] Preferably, the trademark name of the skin barrier multi-repair composition is "Zhi Xiu Yin", and the skin barrier multi-repair composition can be used in the preparation of skin care products.

[0028] Preferably, the facial cream is used in the preparation of a skin care product suitable for sensitive skin that has the effects of moisturizing, repairing, soothing, being mild and non-irritating.

[0029] Preferably, the facial cream is in a cream-like state, white to light yellow in color, with a viscosity of 30,000 to 45,000 mPa.s (sp-4, 10 rpm), and a pH of 5.0 to 7.0.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] (1) The skin barrier multi-repair composition of the present invention utilizes fibronectin to bind to integrin α on the cell surface. v β3 binding, enhances platelet adhesion, phagocyte and fibroblast migration, and cell proliferation, and enhances its adhesion. Fibronectin is an important chemotactic factor for fibroblasts, which can enhance cell adhesion and chemotaxis by helping to maintain the cytoskeleton structure, and is deposited in the intercellular matrix together with type III collagen to form a fibrinogen network structure. The use of ginsenosides and other components to encapsulate fibronectin can further improve the repair effect of the skin barrier multi-repair composition.

[0032] (2) By adding a multi-repairing composition to the cream, the skin can accelerate the absorption of the active ingredients in the cream, promote the secretion of collagen by skin cells, reduce the area of ​​skin red areas, significantly increase the moisture content of the skin stratum corneum, significantly reduce the skin gray value and the skin water loss rate, increase the skin dermal density, dermal thickness and epidermal thickness, reduce epidermal density, and reduce the skin hemoglobin content.

[0033] (3) The multi-repair composition and facial cream of the present invention are suitable for people with sensitive skin, have the characteristics of moisturizing, repairing, soothing, mild and non-irritating, good absorption effect, and the preparation method is simple, does not require large-scale equipment, and has a short production cycle. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 The BCA method was used to test the protein accumulation and transdermal permeation curve at different time points;

[0035] Figure 2 Coomassie Brilliant Blue method - 24-hour protein permeation per unit area;

[0036] Figure 3 This is the result chart of Coomassie Brilliant Blue method-24 hours;

[0037] Figure 4 The repairing effects of the normal control group, the model control group and the cream prepared in Example 6;

[0038] Figure 5 a is skin color, * indicates that the analysis value is significantly different compared with that before use (P<0.05);

[0039] Figure 6 is the red area, * indicates that the analysis value is significantly different compared with that before use (P<0.05);

[0040] Figure 7 is the percentage of red area, * indicates that the analysis value is significantly different from that before use (P<0.05);

[0041] Figure 8 This is the VISIA CR cross-polarized light source-RBX red zone map;

[0042] Fig. 9 is the moisture content of the skin stratum corneum, * indicates that the analysis value is significantly different compared with the value before use (P<0.05);

[0043] Fig.10 is the skin moisture content (average gray value), * indicates that the measured value is significantly different from that before use (P<0.05);

[0044] Fig.11 To collect skin moisture content using Moisturemap MM100 images;

[0045] Fig.12 is the transepidermal water loss rate of the skin, * indicates that the measured value is significantly different from that before use (P<0.05);

[0046] Fig.13 is the dermal density, * indicates that the measured value is significantly different from that before use (P<0.05);

[0047] Fig.14 is the dermis thickness, * indicates that the measured value is significantly different from that before use (P<0.05);

[0048] Fig.15 is the epidermal density, * indicates that the measured value is significantly different from that before use (P<0.05);

[0049] Fig.16 is the epidermal thickness, * indicates that the measured value is significantly different from that before use (P<0.05);

[0050] Fig.17 To collect the transepidermal water loss rate of the skin using Demmalab Ultrasound images;

[0051] Fig.18 is the skin hemoglobin content, * indicates that the measured value is significantly different compared with that before use (P<0.05). DETAILED DESCRIPTION

[0052] The technical solution of the present invention is further described in detail below in conjunction with specific implementation methods.

[0053] Example 1

[0054] A skin barrier multi-repair composition comprises the following components in percentage by mass: 0.4% mannitol, 0.01% trehalose, 0.01% fibronectin, 0.42% phosphatidylcholine, 0.03% ginsenoside, 0.0001% polysorbate 80, 0.26% dipropylene glycol, 0.14% p-hydroxyacetophenone, 0.06% 1,2-hexanediol, 0.04% caprylyl glycol, 0.02% isopentyl glycol, and the balance is water.

[0055] A method for preparing a skin barrier multi-repairing composition comprises the following steps:

[0056] (1) Fibronectin, mannitol, 1,2-hexanediol, dipropylene glycol, p-hydroxyacetophenone and water are mixed and stirred at a temperature of 25° C. to prepare a premix solution;

[0057] (2) mixing ginsenoside, isopentyl glycol, caprylyl glycol, trehalose, phosphatidylcholine and polysorbate 80 at 30° C. to obtain a primary mixed solution;

[0058] (3) The initial mixed solution was added dropwise to the premixed solution at a rate of 0.8 mL / min, and stirred at 300 rpm for 30 min at a temperature of 40° C. to obtain a skin barrier multi-repair composition (fibronectin liposome).

[0059] Example 2

[0060] A skin barrier multi-repair composition comprises the following components in percentage by mass: 14.84% mannitol, 1.53% trehalose, 0.77% fibronectin, 7.32% phosphatidylcholine, 1.31% ginsenoside, 0.1% polysorbate 80, 0.77% dipropylene glycol, 0.41% p-hydroxyacetophenone, 0.17% 1,2-hexanediol, 0.12% caprylyl glycol, 0.05% isoprene glycol, and the balance is water.

[0061] A method for preparing a skin barrier multi-repairing composition comprises the following steps:

[0062] (1) Fibronectin, mannitol, 1,2-hexanediol, dipropylene glycol, p-hydroxyacetophenone and water are mixed and stirred at a temperature of 40° C. to prepare a premix solution;

[0063] (2) mixing ginsenoside, isopentyl glycol, caprylyl glycol, trehalose, phosphatidylcholine and polysorbate 80 at 40° C. to obtain a primary mixed solution;

[0064] (3) The initial mixed solution was added dropwise to the premixed solution at a rate of 1.5 mL / min, and stirred at 400 rpm for 50 min at a temperature of 50° C. to obtain a skin barrier multi-repair composition (fibronectin liposome).

[0065] Example 3

[0066] A skin barrier multi-repair composition comprises the following components in percentage by mass: 7.62% mannitol, 0.76% trehalose, 0.38% fibronectin, 3.86% phosphatidylcholine, 0.67% ginsenoside, 0.001% polysorbate 80, 0.51% dipropylene glycol, 0.27% p-hydroxyacetophenone, 0.11% 1,2-hexanediol, 0.08% caprylyl glycol, 0.03% isoprene glycol, and the balance is water.

[0067] A method for preparing a skin barrier multi-repairing composition comprises the following steps:

[0068] (1) Fibronectin, mannitol, 1,2-hexanediol, dipropylene glycol, p-hydroxyacetophenone and water are mixed and stirred at 30° C. to prepare a premix solution;

[0069] (2) mixing ginsenoside, isopentyl glycol, caprylyl glycol, trehalose, phosphatidylcholine and polysorbate 80 at 35° C. and stirring to obtain a primary mixed solution;

[0070] (3) The initial mixed solution was added dropwise to the premixed solution at a rate of 1.2 mL / min, and stirred at 350 rpm for 40 min at a temperature of 45° C. to obtain a skin barrier multi-repair composition (fibronectin liposome).

[0071] Example 4

[0072] A facial cream, comprising phase A, phase B, phase C and phase D, each phase being mixed according to the following components in percentage by mass, with the remainder being water:

[0073] The phase A comprises 0.01% of macromolecular sodium hyaluronate with a molecular weight of 1 million to 1.8 million Da, 0.02% of medium molecular sodium hyaluronate with a molecular weight of 200,000 to 400,000 Da, 0.04% of small molecular sodium hyaluronate with a molecular weight of less than 10,000 Da, and 0.10% of carbomer;

[0074] The phase B includes 0.01% skin barrier multi-repair composition, 0.10% dipotassium glycyrrhizinate, 1% betaine, 2% 1,2-pentanediol, 0.02% sphingomonas fermentation product extract, 2% glycerol, 0.5% 1,2-hexanediol, and 0.1% raspberry ketone;

[0075] The phase C includes 0.2% cetearyl glucoside, 1% eicosyl glucoside, 0.1% behenyl alcohol, 1.08% dimethicone, and 0.22% sodium polyacryloyldimethyl taurate;

[0076] The phase D comprises 1% damask rose flower water, 0.5% p-hydroxyacetophenone, 0.5% 1,2-hexanediol, and 0.1% aminomethyl propanol;

[0077] The skin barrier multi-repair composition is prepared according to the components and method of Example 1.

[0078] A method for preparing a facial cream comprises the following steps:

[0079] (1) Add water to the phase A component and stir at 35 rpm for 15 min to swell and mix, then add the phase B component, heat to 75°C, and stir at 30 rpm until completely dissolved to obtain a phase AB mixture;

[0080] (2) Stir the components of phase C at 75°C and 25 rpm for 30 min to mix them evenly;

[0081] (3) Mix the AB phase mixture and the C phase components, homogenize at 2500 rpm for 12 min, and then continue stirring at 25 rpm for 20 min to obtain an ABC phase mixture;

[0082] (4) The mixture of phases ABC was stirred at 25 rpm and cooled to 40°C, and the components of phase D were added and stirred until the temperature reached 22°C. The mixture was degassed and filtered through a 150-mesh filter to obtain a facial cream.

[0083] Example 5

[0084] A facial cream, comprising phase A, phase B, phase C and phase D, each phase being mixed according to the following components in percentage by mass, with the remainder being water:

[0085] The phase A includes 0.05% of macromolecular sodium hyaluronate with a molecular weight of 1 million to 1.8 million Da, 0.10% of medium-molecular sodium hyaluronate with a molecular weight of 200,000 to 400,000 Da, 0.20% of small-molecule sodium hyaluronate with a molecular weight of less than 10,000 Da, and 0.30% of carbomer; the phase B includes 5% of skin barrier multi-repair composition, 1.0% of dipotassium glycyrrhizinate, 4% of betaine, 6% of 1,2-pentanediol, 1% of sphingomonas fermentation product extract, 7% of glycerol, 2% of 1,2-hexanediol, and 0.6% of raspberry ketone;

[0086] The phase C includes 0.8% cetearyl glucoside, 5% eicosyl glucoside, 0.5% behenyl alcohol, 5% dimethicone, and 1.25% sodium polyacryloyldimethyl taurate;

[0087] The phase D includes 4% damask rose flower water, 2% p-hydroxyacetophenone, 2% 1,2-hexanediol, and 0.2% aminomethyl propanol;

[0088] The skin barrier multi-repair composition is prepared according to the components and method of Example 2.

[0089] A method for preparing a facial cream comprises the following steps:

[0090] (1) Add water to the phase A component and stir at 45 rpm for 25 min to swell and mix, then add the phase B component, heat to 85°C, and stir at 40 rpm until completely dissolved to obtain a phase AB mixture;

[0091] (2) Stir the components of phase C at 85°C and 35 rpm for 40 min to mix them evenly;

[0092] (3) Mix the AB phase mixture and the C phase components, homogenize at 3000 rpm for 20 min, and then continue stirring at 35 rpm for 40 min to obtain an ABC phase mixture;

[0093] (4) The ABC mixture was stirred at 35 rpm and cooled to 50°C, and the D phase component was added and continued to be stirred to 26°C. The mixture was degassed and filtered through a 200-mesh filter to obtain a facial cream.

[0094] Example 6

[0095] A facial cream, comprising phase A, phase B, phase C and phase D, each phase being mixed according to the following components in percentage by mass, with the remainder being water:

[0096] The phase A comprises 0.02% of macromolecular sodium hyaluronate with a molecular weight of 1 million to 1.8 million Da, 0.04% of medium molecular sodium hyaluronate with a molecular weight of 200,000 to 400,000 Da, 0.06% of small molecular sodium hyaluronate with a molecular weight of less than 10,000 Da, and 0.25% of carbomer;

[0097] The phase B includes 3.0% skin barrier multi-repair composition, 0.15% dipotassium glycyrrhizinate, 2% betaine, 3% 1,2-pentanediol, 0.04% sphingomonas fermentation product extract, 3.5% glycerol, 1.0% 1,2-hexanediol, and 0.4% raspberry ketone;

[0098] The phase C includes 0.5% cetearyl glucoside, 2.5% eicosyl glucoside, 0.3% behenyl alcohol, 2.0% dimethicone, and 0.5% sodium polyacryloyldimethyl taurate;

[0099] The phase D comprises 3% damask rose flower water, 1.0% p-hydroxyacetophenone, 1.0% 1,2-hexanediol, and 0.15% aminomethyl propanol;

[0100] The skin barrier multi-repair composition is prepared according to the components and method of Example 3.

[0101] A method for preparing a facial cream comprises the following steps:

[0102] (1) Add water to the phase A component and stir at 40 rpm for 20 min to swell and mix, add the phase B component, heat to 82°C, and stir at 32 rpm until completely dissolved to obtain a phase AB mixture;

[0103] (2) Stir the components of phase C at 80°C and 28 rpm for 35 min to mix them evenly;

[0104] (3) Mix the AB phase mixture and the C phase components, homogenize at 2700 rpm for 15 min, and then continue stirring at 30 rpm for 35 min to obtain an ABC phase mixture;

[0105] (4) The mixture of phases ABC was stirred at 30 rpm and cooled to 45°C, and the components of phase D were added and stirred continuously until the temperature reached 25°C. The mixture was degassed and filtered through a 180-mesh filter to obtain a facial cream.

[0106] Comparative Example 1

[0107] The difference between this comparative example and Example 6 is that the fibronectin in the skin barrier multi-repair composition is replaced with soluble collagen, and the other ingredients and steps remain unchanged.

[0108] A skin barrier multi-repair composition comprises the following components in percentage by mass: 7.62% mannitol, 0.76% trehalose, 0.38% soluble collagen, 3.86% phosphatidylcholine, 0.67% ginsenoside, 0.001% polysorbate 80, 0.51% dipropylene glycol, 0.27% p-hydroxyacetophenone, 0.11% 1,2-hexanediol, 0.08% caprylyl glycol, 0.03% isopentyl glycol, and the balance is water.

[0109] A facial cream, comprising phase A, phase B, phase C and phase D, each phase being mixed according to the following components in percentage by mass, with the remainder being water:

[0110] The phase A comprises 0.02% of macromolecular sodium hyaluronate with a molecular weight of 1 million to 1.8 million Da, 0.04% of medium molecular sodium hyaluronate with a molecular weight of 200,000 to 400,000 Da, 0.06% of small molecular sodium hyaluronate with a molecular weight of less than 10,000 Da, and 0.25% of carbomer;

[0111] The phase B includes 3.0% skin barrier multi-repair composition, 0.15% dipotassium glycyrrhizinate, 2% betaine, 3% 1,2-pentanediol, 0.04% sphingomonas fermentation product extract, 3.5% glycerol, 1.0% 1,2-hexanediol, and 0.4% raspberry ketone;

[0112] The phase C includes 0.5% cetearyl glucoside, 2.5% eicosyl glucoside, 0.3% behenyl alcohol, 2.0% dimethicone, and 0.5% sodium polyacryloyldimethyl taurate;

[0113] The phase D comprises 3% damask rose flower water, 1.0% p-hydroxyacetophenone, 1.0% 1,2-hexanediol, and 0.15% aminomethyl propanol;

[0114] Comparative Example 2

[0115] This comparative example is different from Example 6 in that the skin barrier multi-repair composition is replaced with fibronectin, and the other ingredients and steps remain unchanged.

[0116] A facial cream, comprising phase A, phase B, phase C and phase D, each phase being mixed according to the following components in percentage by mass, with the remainder being water:

[0117] The phase A comprises 0.02% of macromolecular sodium hyaluronate with a molecular weight of 1 million to 1.8 million Da, 0.04% of medium molecular sodium hyaluronate with a molecular weight of 200,000 to 400,000 Da, 0.06% of small molecular sodium hyaluronate with a molecular weight of less than 10,000 Da, and 0.25% of carbomer;

[0118] The phase B includes 3.0% fibronectin, 0.15% dipotassium glycyrrhizinate, 2% betaine, 3% 1,2-pentanediol, 0.04% sphingomonas fermentation product extract, 3.5% glycerol, 1.0% 1,2-hexanediol, and 0.4% raspberry ketone;

[0119] The phase C includes 0.5% cetearyl glucoside, 2.5% eicosyl glucoside, 0.3% behenyl alcohol, 2.0% dimethicone, and 0.5% sodium polyacryloyldimethyl taurate;

[0120] The phase D comprises 3% damask rose flower water, 1.0% p-hydroxyacetophenone, 1.0% 1,2-hexanediol, and 0.15% aminomethyl propanol;

[0121] Comparative Example 3

[0122] The difference between this comparative example and Example 6 is that the dropping rate in the preparation method of the skin barrier multi-repairing composition is increased and changed, and the other ingredients and steps remain unchanged.

[0123] A method for preparing a skin barrier multi-repairing composition comprises the following steps:

[0124] (1) Fibronectin, mannitol, 1,2-hexanediol, dipropylene glycol, p-hydroxyacetophenone and water are mixed and stirred at a temperature of 25° C. to prepare a premix solution;

[0125] (2) mixing ginsenoside, isopentyl glycol, caprylyl glycol, trehalose, phosphatidylcholine and polysorbate 80 at 30° C. to obtain a primary mixed solution;

[0126] (3) The initial mixed solution was added dropwise to the premixed solution at a rate of 3 mL / min, and stirred at 300 rpm for 30 min at a temperature of 40° C. to obtain a skin barrier multi-repairing composition.

[0127] Comparative Example 4

[0128] The difference between this comparative example and Example 6 is that the facial cream does not contain the skin barrier multi-repairing composition, and the other ingredients and steps remain unchanged.

[0129] A facial cream, comprising phase A, phase B, phase C and phase D, each phase being mixed according to the following components in percentage by mass:

[0130] The phase A comprises 0.02% of macromolecular sodium hyaluronate with a molecular weight of 1 million to 1.8 million Da, 0.04% of medium molecular sodium hyaluronate with a molecular weight of 200,000 to 400,000 Da, 0.06% of small molecular sodium hyaluronate with a molecular weight of less than 10,000 Da, and 0.25% of carbomer;

[0131] The phase B includes 0.15% dipotassium glycyrrhizinate, 2% betaine, 3% 1,2-pentanediol, 0.04% sphingomonas fermentation product extract, 3.5% glycerol, 1.0% 1,2-hexanediol, and 0.4% raspberry ketone;

[0132] The phase C includes 0.5% cetearyl glucoside, 2.5% eicosyl glucoside, 0.3% behenyl alcohol, 2.0% dimethicone, and 0.89% sodium polyacryloyldimethyl taurate;

[0133] The phase D comprises 3% damask rose flower water, 1.0% p-hydroxyacetophenone, 1.0% 1,2-hexanediol, and 0.15% aminomethyl propanol;

[0134] Comparative Example 5

[0135] This comparative example is different from Example 6 in that the cream does not contain phase B, and the other ingredients and steps remain unchanged.

[0136] A facial cream, comprising phase A, phase C and phase D, each phase being mixed according to the following components in percentage by mass:

[0137] The phase A comprises 0.02% of macromolecular sodium hyaluronate with a molecular weight of 1 million to 1.8 million Da, 0.04% of medium molecular sodium hyaluronate with a molecular weight of 200,000 to 400,000 Da, 0.06% of small molecular sodium hyaluronate with a molecular weight of less than 10,000 Da, and 0.25% of carbomer;

[0138] The phase C includes 0.5% cetearyl glucoside, 2.5% eicosyl glucoside, 0.3% behenyl alcohol, 2.0% dimethicone, and 0.89% sodium polyacryloyldimethyl taurate;

[0139] The phase D comprises 3% damask rose flower water, 1.0% p-hydroxyacetophenone, 1.0% 1,2-hexanediol, and 0.15% aminomethyl propanol;

[0140] Comparative Example 6

[0141] This comparative example is different from Example 6 in that the facial cream does not contain phase C, and the other ingredients and steps remain unchanged.

[0142] A facial cream, comprising phase A, phase B and phase D, each phase being mixed according to the following components in percentage by mass:

[0143] The phase A comprises 0.02% of macromolecular sodium hyaluronate with a molecular weight of 1 million to 1.8 million Da, 0.04% of medium molecular sodium hyaluronate with a molecular weight of 200,000 to 400,000 Da, 0.06% of small molecular sodium hyaluronate with a molecular weight of less than 10,000 Da, and 0.25% of carbomer;

[0144] The phase B includes 0.15% dipotassium glycyrrhizinate, 2% betaine, 3% 1,2-pentanediol, 0.04% sphingomonas fermentation product extract, 3.5% glycerol, 1.0% 1,2-hexanediol, and 0.4% raspberry ketone;

[0145] The phase D comprises 3% damask rose flower water, 1.0% p-hydroxyacetophenone, 1.0% 1,2-hexanediol, and 0.15% aminomethyl propanol;

[0146] Experimental Example 1 Preliminary Study on Saponin Liposome-Assisted Transdermal Transfection of Fibronectin

[0147] 1.1 Experimental materials: ordinary liposome group (Lip) prepared with egg yolk egg gel, fibronectin liposome group (R-Lip) prepared in Example 3 of the present application, and free fibronectin aqueous solution group (Pro);

[0148] 1.2 Test reagents: chloroform, ether, BCA protein content determination kit, Coomassie brilliant blue protein content detection kit;

[0149] 1.3 Test method:

[0150] Preparation of ex vivo mouse skin: There were 10 mice in each experimental group. The mice were anesthetized and fixed, and the hair on the abdomen of the mice was shaved with an electric shaver. After being killed by cervical dislocation, the abdominal skin was immediately cut off and placed on a tray. The subcutaneous fat and mucous tissue were carefully removed, and the skin was repeatedly rinsed with PBS and frozen at -20℃ for later use.

[0151] In vitro permeation test method: The in vitro transdermal absorption test was performed using a Franz test diffusion apparatus (diffusion area: 3.14 cm2, diffusion cell volume: 8 mL). The diffusion cell consists of two glass components, with mouse skin sandwiched in the middle to form an upper and lower chamber. The upper chamber is the supply cell and the lower chamber is the receiving cell, which is placed in a (37±0.5)℃ constant temperature water bath. 1 ml of each of the common liposome group (Lip), saponin liposome group (R-Lip), and protein aqueous solution (Pro) were added to the upper chamber, and PBS (receiving medium) was added to the receiving cell. The bubbles were removed so that the dermis was in complete contact with the receiving medium. 0.2 mL of the receiving solution was taken out at 0, 0.5, 1, 2, 4, 6, 12, and 24 hours, and an equal amount of isothermal fresh receiving medium was added to the receiving cell. After the removed receiving solution was filtered through a 0.45μm microporous membrane, the average protein content was determined using a BCA kit, and the average protein content at 0h and 24h was determined by the Coomassie Brilliant Blue method to obtain the protein permeation per unit area.

[0152] 1.4 Test Results

[0153] 1.4.1 The absorbance and protein accumulation curves at different time points measured by BCA kit

[0154] The absorbance is shown in Table 1, and the protein accumulation curve is shown in Figure 1 As shown:

[0155] Table 1 Absorbance test

[0156]

[0157] 1.4.2 Determination of protein transdermal permeation per unit area in 24 hours by Coomassie Brilliant Blue method

[0158] The amount of protein permeated per unit area is Figure 2 The results are shown in the figure Figure 3 As shown;

[0159] Conclusion: The transdermal results showed that the fibronectin in the fibronectin liposome group could penetrate the epidermis to reach the dermis, and the transdermal effect was better than that of other common liposome groups and free fibronectin aqueous solution groups. The protein content was measured after 24 hours, and the fibronectin liposome group had a very significant difference compared with the common liposome and free protein groups. The protein cumulative permeation per unit area measured by the BCA method was about 2.16 times that of the common liposome group.

[0160] Experimental Example 2 Repair Effect

[0161] 2.1 Experimental Materials

[0162] The skin barrier multi-repair composition prepared in Examples 1 to 3, and the facial cream prepared in Examples 4 to 6 and Comparative Examples 1 to 6; 210 wild-type AB zebrafish 3 days after fertilization;

[0163] 2.2 Principle and method

[0164] The regeneration of the zebrafish tail fin is divided into three processes: wound healing, blastema formation, and regeneration outcome, with blastema formation being the core. There are multiple sources of cells for fin regeneration, including epidermal cells, fibroblasts, and osteoblasts, all of which promote the regeneration of the tail fin, and these cells are highly lineage-restricted. The regeneration of the zebrafish tail fin is similar to the repair mechanism of human skin, bones, and blood vessels. The zebrafish tail fin has become an important model for studying the tissue regeneration process because of its simple structure, easy surgical operation, no impact on survival after surgery, and easy observation. The tail fin was cut off perpendicular to the trunk with a scalpel, and the quantitative results of the area of ​​the zebrafish regenerated tail fin were used to evaluate the repair efficacy of the sample.

[0165] 2.3 Test Method

[0166] (1) Use a scalpel to remove the zebrafish tail fin to establish a zebrafish tail fin injury model.

[0167] (2) Model zebrafish were randomly assigned to 6-well plates, with 15 zebrafish per well.

[0168] (3) After the water-soluble multi-repairing composition or cream was applied, the sample was administered. A normal control group (normal zebrafish feeding), a model control group (zebrafish model + no sample treatment), and an experimental group (zebrafish model + treatment with the repairing composition / cream) were set up. The volume of each well was 3 mL.

[0169] (4) Incubate at 28°C in the dark for 48 h.

[0170] (5) Ten zebrafish were randomly selected from each experimental group and photographed under a dissecting microscope. Advanced image processing software was used to analyze and collect data. The tail fin area (A) of the zebrafish was analyzed and the formula was used to calculate and determine whether the sample had a repair effect.

[0171]

[0172] 2.4 Test Results

[0173] The average test data are shown in Table 2. The effects of the normal control group, the model control group and the cream prepared in Example 6 are shown in Table 2. Figure 4 As shown:

[0174] Table 2 Repair effect

[0175]

[0176]

[0177] As can be seen from Table 2, the multiple repair compositions prepared in Examples 1 to 3 of the present invention and the creams prepared in Examples 4 to 6 all have good repair effects, especially Example 6, which can promote the regeneration of damaged tail fins; by comparing Examples 1 to 3, Examples 4 to 6 and Comparative Example 4, it can be seen that the repair effect of the cream containing the repair composition is 39.98 to 43.01%, the repair effect of the cream containing only the repair composition is 18.44 to 19.33%, and the repair effect of the cream without the repair composition is 3.45%, indicating that the repair effect of the cream made by adding components on the basis of the multiple repair composition is greatly improved compared with the repair composition alone or the cream excipient alone. By comparing Example 6 with Comparative Examples 1 and 2, it can be seen that the fibronectin encapsulated therein is more effective than soluble collagen, and components such as ginsenosides are used to form a liposome membrane to encapsulate fibronectin. The liposome membrane is similar to the cell membrane and can be inserted into the lipid layer of the cell membrane. The fibronectin encapsulated therein is directly injected into the cell, making the cream safer and milder, and the absorption efficiency of the effective ingredients is higher. Comparison between Example 6 and Comparative Example 3 shows that the acceleration rate of the initial mixed droplets is too fast, resulting in incomplete fibronectin wrapping, affecting the repair effect; comparison between Example 6 and Comparative Examples 5 and 6 shows that the cream can achieve the maximum repair effect only when all phase components are added completely.

[0178] Experimental Example 3: Instrumental Test Efficacy and Subject Self-Assessment

[0179] 3.1 Experimental materials: facial cream prepared in Example 6;

[0180] 3.2 Evaluation Parameters:

[0181] (1) VISIA CR is used for facial image acquisition and red zone analysis;

[0182] (2) Corneometer CM825 is used to measure the moisture content of the stratum corneum of the skin;

[0183] (3) Moisturemap MM100 is used to detect skin moisture content;

[0184] (4) Tewameter TM300 is used to detect the transepidermal water loss rate of the skin;

[0185] (5) Demmalab Ultrasound is used for facial image acquisition and dermis and epidermis analysis;

[0186] (6) Skin melanin and hemoglobin content meter Mexameter MX18 is used to detect skin hemoglobin content;

[0187] (7) Self-assessment by subjects.

[0188] 3.3 Test Method

[0189] Subjects: A total of 32 valid persons completed the assessment, including healthy Chinese female subjects with sensitive skin (screened by questionnaire), aged 32 to 59 years old, with an average age of 46.53±7.03, facial redness level 3 to 69 (according to SGS standard atlas), moisture content of the stratum corneum of the random cheek side <50 C.U. (Corneometer measurement); transepidermal water loss rate of the random cheek side>15g / Nm' (Tewameter measurement); and met the voluntary inclusion and exclusion criteria of the subjects.

[0190] Areas assessed: Face

[0191] How to use: After cleansing and toning every morning and evening, take an appropriate amount of this product and pat it evenly on the face, avoiding the skin around the eyes, and gently massage until absorbed, for 28 consecutive days.

[0192] Solution design:

[0193] (1) Before the evaluation

[0194] The experimenter screened the subjects who met the assessment requirements and recorded the subjects' Fitzpatrick type and skin type (dry, mixed to dry, normal, oily, mixed to oily);

[0195] (2) Before using the product (DO)

[0196] The subjects cleaned their faces using cleansing products

[0197] The subjects waited for 30 minutes in a laboratory environment with a temperature of 21±2℃ and a relative humidity of 50±10%RH.

[0198] Experts conduct inclusion assessment

[0199] The experimenter collected images using VISIA CR, Demmalab Ultrasound, and Moisturemap MM100. The experimenter measured the moisture content of the stratum corneum and the transepidermal water loss rate of the subjects' cheeks.

[0200] Subjects completed a self-assessment questionnaire

[0201] The experimenter distributed the product and the usage log, and the subjects used the product according to the instructions;

[0202] (3) Immediately after using the product (D0Timm)

[0203] Subjects completed a self-assessment questionnaire

[0204] The subjects waited for 15 minutes in a laboratory environment with a temperature of 21±2°C and a relative humidity of 50±10%RH;

[0205] (4) 15 minutes after using the product (D0T15min)

[0206] The experimenter measured the moisture content of the stratum corneum on the cheek of the subject and the subject filled in the self-assessment questionnaire

[0207] (5) 24 hours after using the product (D0T24h) (online)

[0208] Subjects completed a self-assessment questionnaire

[0209] (6) 14 days after using the product (D14)

[0210] The subjects cleaned their faces with cleansing products. The subjects waited for 30 minutes in a laboratory environment with a temperature of 21±2℃ and a relative humidity of 50±10%RH. The experimenter collected images using VISIA CR, Demmalab Ultrasound, and Moisturemap MM100. The experimenter measured the moisture content of the stratum corneum and the transepidermal water loss rate of the subjects' cheeks. The subjects filled out a self-assessment questionnaire.

[0211] (7) 28 days after using the product (D28)

[0212] The subjects cleaned their faces using cleansing products

[0213] The subjects waited for 30 minutes in a laboratory environment with a temperature of 21±2℃ and a relative humidity of 50±10%RH. The experimenter collected images using VISIACR, Demmalab Ultrasound, and Moisturemap MM100. The experimenter measured the moisture content of the stratum corneum and the transepidermal water loss rate of the subjects' cheeks. The subjects filled out a self-assessment questionnaire.

[0214] (8) Data statistics: SPSS 28.0 was used for statistical analysis. The data were tested for normal distribution. If the data were normally distributed, the paired t-test method was used for statistical analysis; if the data were non-normally distributed, the Wicoxon signed rank test method was used for statistical analysis. The Wilcoxon signed rank test was used for statistical analysis of rank data. The statistical method significance level was P < 0.05.

[0215] 3.4 Test Results

[0216] 3.4.1 Red Zone (VISIACR) (Cheek)

[0217] like Figure 5 , Figure 6 , Figure 7 and Figure 8 As shown, a smaller value indicates that the red area has improved compared to before using the product:

[0218] After using the product for 14 days, the a* value of the skin color in the cheek evaluation area decreased significantly by 15.67%, the red area decreased significantly by 14.47%, and the proportion of the red area decreased significantly by 14.47%.

[0219] After using the product for 28 days, the a* value of the skin color in the cheek evaluation area decreased significantly by 18.12%, the red area decreased significantly by 12.70%, and the proportion of the red area decreased significantly by 12.70%.

[0220] 3.4.2 Skin stratum corneum moisture content (Corneometer CM825) (cheek)

[0221] like Fig. 9 As shown, the larger the value, the more moisture content of the stratum corneum of the skin increases. The rate of change = ((average value after using the product - average value before using the product) ÷ average value before using the product) × 100%;

[0222] Compared with before using the product: 15 minutes after using the product, the moisture content of the stratum corneum of the skin in the cheek evaluation area increased significantly by 58.83%; 14 days after using the product, the moisture content of the stratum corneum of the skin in the cheek evaluation area increased significantly by 21.49%; after 28 days of using the product, the moisture content of the stratum corneum of the skin in the cheek evaluation area increased significantly by 35.11%.

[0223] 3.4.3 Skin moisture content (Moisturemap MM100) (cheek)

[0224] like Fig.10 and 11 As shown, a smaller value indicates an increase in skin moisture content, and the rate of change = ((average value after using the product - average value before using the product) ÷ average value before using the product) × 100%;

[0225] Compared with before using the product: After using the product for 14 days, the average gray value of the cheek evaluation area decreased significantly by 5.68%. After using the product for 28 days, the average gray value of the cheek evaluation area decreased significantly by 12.14%.

[0226] 3.4.4 Transepidermal water loss rate (Tewameter TM300) (cheek)

[0227] like Fig.12As shown, a smaller value indicates that the skin barrier function has improved, and the change rate = ((average value after using the product - average value before using the product) ÷ average value before using the product) × 100%;

[0228] Compared with before using the product: After using the product for 14 days, the transepidermal water loss rate of the cheek evaluation area was significantly reduced by 12.58%. After using the product for 28 days, the transepidermal water loss rate of the cheek evaluation area was significantly reduced by 19.55%.

[0229] 3.4.5 Facial image acquisition and dermis and epidermis analysis (Demmalab Ultrasound) (cheek)

[0230] like Figures 13 to 17 As shown, the increase in the dermis / epidermis analysis value indicates that the dermis / epidermis has improved, and the change rate = ((average value after using the product - average value before using the product) ÷ average value before using the product) × 100%;

[0231] Compared with before using the product: After using the product for 14 days, the dermal density of the cheek evaluation area increased by 3.91%, the dermal thickness increased by 2.25%, the epidermal density decreased by 1.59%, and the epidermal thickness increased by 17.89%. After using the product for 28 days, the dermal density of the cheek evaluation area increased significantly by 9.47%, the dermal thickness decreased by 0.67%, the epidermal density decreased by 2.58%, and the epidermal thickness increased significantly by 16.06%.

[0232] 3.4.6 Skin hemoglobin content (Mexameter MX18) (cheek)

[0233] like Fig.18 As shown, the decrease in skin hemoglobin content indicates that the skin hemoglobin content is reduced, and the change rate = ((average value after using the product - average value before using the product) ÷ average value before using the product) × 100%;

[0234] Compared with before using the product: after using the product for 14 days, the hemoglobin content in the skin of the cheek evaluation area was significantly reduced by 5.70%; after using the product for 28 days, the hemoglobin content in the skin of the cheek evaluation area was significantly reduced by 14.26%.

[0235] 3.4.7 Subject Self-Assessment

[0236] As shown in Table 3, Table 4 and Table 5:

[0237] Table 3 Self-assessment results of subjects (skin condition)

[0238]

[0239]

[0240] Scoring standard: 0-9 points, 0 points means very good, 1-3 points means good, 4-5 points means average, 6-8 points means bad, and 9 points means very bad.

[0241] Compared with before using the product: After using the product for 14 days, the mean self-rating of the subjects' skin dryness decreased significantly by 4.56, the mean self-rating of the subjects' skin redness decreased significantly by 3.94, the mean self-rating of the subjects' overall skin condition decreased significantly by 4.53, the mean self-rating of the subjects' tolerance to external stimuli decreased significantly by 3.91, the mean self-rating of the subjects' skin sensitivity (i.e. whether it is easily affected by external conditions) decreased significantly by 3.53, the mean self-rating of the subjects' skin irritation / discomfort decreased significantly by 3.69, the mean self-rating of the subjects' skin itching degree or frequency decreased significantly by 3.81, the mean self-rating of the subjects' skin tingling degree or frequency decreased significantly by 4.13, and the mean self-rating of the subjects' skin burning degree or frequency decreased significantly by 4.09. After using the product for 28 days, the mean self-rating of the subjects' skin dryness decreased by 544, the mean self-rating of the subjects' skin redness decreased by 5.06, the mean self-rating of the subjects' overall skin condition decreased by 5.22, the mean self-rating of the subjects' tolerance to external stimuli decreased by 4.72, the mean self-rating of the subjects' skin sensitivity (i.e. whether it is easily affected by external conditions) decreased by 4.53, the mean self-rating of the subjects' skin irritation / discomfort decreased by 4.34, the mean self-rating of the subjects' skin itching degree or frequency decreased by 4.56, the mean self-rating of the subjects' skin tingling degree or frequency decreased by 4.66, and the mean self-rating of the subjects' skin burning degree or frequency decreased by 4.91.

[0242] Table 4 Results of self-assessment by subjects (degree of identification) (short term)

[0243]

[0244]

[0245] Scoring criteria: 1 point means strongly disagree, 2 points means somewhat disagree, 3 points means average, 4 points means somewhat agree, and 5 points means strongly agree.

[0246] Recognition degree = number of subjects with a score > 3 points / number of valid subjects x 100%

[0247] Immediately after using the product, 94% of the subjects felt that skin dryness was improved after use, 72% of the subjects felt that skin stinging was improved after use, 72% of the subjects felt that skin burning was improved after use, and 63% of the subjects felt that skin redness was reduced after use.

[0248] 15 minutes after using the product, 100% of the subjects felt that skin dryness was improved after use, 91% of the subjects felt that skin stinging was improved after use, 94% of the subjects felt that skin burning was improved after use, and 78% of the subjects felt that skin redness was reduced after use.

[0249] 24 hours after using the product, 97% of the subjects felt that skin dryness was improved after use, 97% of the subjects felt that skin stinging was improved after use, 81% of the subjects felt that skin burning was improved after use, and 72% of the subjects felt that skin redness was reduced after use.

[0250] Table 5 Self-assessment results of subjects (degree of identification) (long-term)

[0251]

[0252] Scoring criteria: 1 point means strongly disagree, 2 points means somewhat disagree, 3 points means average, 4 points means somewhat agree, and 5 points means strongly agree.

[0253] Degree of recognition = Number of subjects with a score > 3: Number of valid subjects x 100%

[0254] After using the product for 14 days, 100% of the subjects believed that it deeply hydrated and moisturized the skin after use, 100% of the subjects believed that it increased the skin's water content after use, 97% of the subjects believed that it deeply locked in moisture after use, 94% of the subjects believed that the skin was lastingly hydrated after use, 97% of the subjects believed that it soothed skin discomfort after use, 97% of the subjects believed that it reduced skin irritation after use, 97% of the subjects believed that the skin barrier was repaired after use, 100% of the subjects believed that the skin's resistance was enhanced after use, and 100% of the subjects believed that the product was gentle and non-irritating.

[0255] After using the product for 28 days, 100% of the subjects believed that it deeply hydrated and moisturized their skin after use, 100% of the subjects believed that it increased the skin's water content after use, 100% of the subjects believed that it deeply locked in moisture after use, 100% of the subjects believed that their skin was lastingly hydrated after use, 97% of the subjects believed that it soothed skin discomfort after use, 100% of the subjects believed that it reduced skin irritation after use, 100% of the subjects believed that it repaired the skin barrier after use, 100% of the subjects believed that it enhanced the skin's resistance after use, 100% of the subjects believed that the product was gentle and non-irritating, 100% of the subjects were satisfied with the overall effect / efficacy of the product after use, and 100% of the subjects were willing to continue using the product.

[0256] 3.5 Evaluation conclusion:

[0257] 3.5.1 Moisturizing

[0258] Compared with before using the product, the moisture content of the stratum corneum of the skin in the cheek evaluation area was significantly improved 14 days after using the product and 28 days after using the product. The evaluation results show that under this evaluation condition, the evaluated product has a moisturizing effect.

[0259] 3.5.2 Repair

[0260] Compared with before using the product, the moisture content of the stratum corneum in the cheek evaluation area, the transepidermal water loss rate, the red zone area (image analysis), the red zone area ratio (image analysis), and the skin hemoglobin content were significantly improved 14 days after using the product and 28 days after using the product. The evaluation results show that under this evaluation condition, the evaluated product has a repairing effect.

[0261] 3.5.3. Soothing

[0262] Compared with before using the product, 14 days after using the product and 28 days after using the product, the a* value of skin color in the cheek evaluation area (image analysis), the red area (image analysis), the proportion of the red area (image analysis), the skin hemoglobin content, and the scores of soothing-related questions in the consumer usage test were significantly improved. The evaluation results show that under this evaluation condition, the evaluated product has a soothing effect.

[0263] 3.5.4. Mild (non-irritating)

[0264] Compared with before using the product, there was no significant increase in the scores of consumers on mildness (non-irritation) related questions in the test questionnaire 14 days after using the product and 28 days after using the product. The evaluation results show that under this evaluation condition, the product is mild (non-irritating).

[0265] 3.5.5. Suitable for sensitive skin

[0266] Compared with before using the product, there was no significant increase in the scores of consumers on sensitive skin-related issues in the test questionnaire 14 days after using the product and 28 days after using the product. The evaluation results show that under this evaluation condition, the product is mild (non-irritating).

[0267] 3.5.6 Adverse skin reactions

[0268] During the use of the product, no subjects experienced local or systemic adverse skin reactions.

[0269] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A skin barrier multi-repair composition, characterized in that: The invention comprises the following components in percentage by mass: 0.4-14.84% mannitol, 0.01-1.53% trehalose, 0.01-0.77% fibronectin, 0.42-7.32% phosphatidylcholine, 0.03-1.31% ginsenoside, 0.0001-0.1% polysorbate-80, 0.26-0.77% dipropylene glycol, 0.14-0.41% p-hydroxyacetophenone, 0.06-0.17% 1,2-hexanediol, 0.04-0.12% caprylyl glycol, 0.02-0.05% isoprene glycol, and the balance is water.

2. The skin barrier multi-repair composition according to claim 1, characterized in that: The invention comprises the following components in percentage by mass: 7.62% mannitol, 0.76% trehalose, 0.38% fibronectin, 3.86% phosphatidylcholine, 0.67% ginsenoside, 0.001% polysorbate 80, 0.51% dipropylene glycol, 0.27% p-hydroxyacetophenone, 0.11% 1,2-hexanediol, 0.08% caprylyl glycol, 0.03% isoprene glycol, and the balance is water.

3. The method for preparing a skin barrier multi-repairing composition according to claim 1, characterized in that: The steps include: (1) Fibronectin, mannitol, 1,2-hexanediol, dipropylene glycol, p-hydroxyacetophenone and water are mixed and stirred at a temperature of 25 to 40° C. to prepare a premix solution; (2) mixing ginsenoside, isopentyl glycol, caprylyl glycol, trehalose, phosphatidylcholine and polysorbate 80 at a temperature of 30 to 40° C. to obtain a primary mixed solution; (3) The initial mixed solution is added dropwise to the premixed solution at a rate of 0.8 to 1.5 mL / min, and stirred at 300 to 400 rpm for 30 to 50 min at a temperature of 40 to 50° C. to obtain a skin barrier multi-repairing composition.

4. A facial cream, characterized in that: The facial cream comprises phase A, phase B, phase C and phase D, each phase being mixed with the following components in percentage by mass, with the remainder being water: The phase A comprises 0.01-0.05% of a macromolecular sodium hyaluronate with a molecular weight of 1 million to 1.8 million Da, 0.02-0.10% of a medium molecular sodium hyaluronate with a molecular weight of 200,000 to 400,000 Da, 0.04-0.20% of a small molecular sodium hyaluronate with a molecular weight of less than 10,000 Da, and 0.10-0.30% of carbomer; The phase B comprises 0.01-5% of the skin barrier multi-repair composition of claim 1, 0.10-1.0% dipotassium glycyrrhizinate, 1-4% betaine, 2-6% 1,2-pentanediol, 0.02-1% sphingomonas fermentation product extract, 2-7% glycerol, 0.5-2% 1,2-hexanediol, and 0.1-0.6% raspberry ketone; The phase C comprises 0.2-0.8% cetearyl glucoside, 1-5% eicosyl glucoside, 0.1-0.5% behenyl alcohol, 1.05-5% polydimethylsiloxane, and 0.22-1.25% sodium polyacryloyl dimethyl taurate; The phase D comprises 1-4% of damask rose flower water, 0.5-2% of p-hydroxyacetophenone, 0.5-2% of 1,2-hexanediol and 0.1-0.2% of aminomethyl propanol.

5. The facial cream according to claim 4, characterized in that The facial cream comprises phase A, phase B, phase C and phase D, each phase being mixed with the following components in percentage by mass, with the remainder being water: The phase A comprises 0.02% of macromolecular sodium hyaluronate with a molecular weight of 1 million to 1.8 million Da, 0.04% of medium molecular sodium hyaluronate with a molecular weight of 200,000 to 400,000 Da, 0.06% of small molecular sodium hyaluronate with a molecular weight of less than 10,000 Da, and 0.25% of carbomer; The phase B comprises 3% of the skin barrier multi-repair composition of claim 1, 0.15% dipotassium glycyrrhizinate, 2% betaine, 3% 1,2-pentanediol, 0.04% Sphingomonas fermentation product extract, 3.5% glycerol, 1% 1,2-hexanediol, and 0.4% raspberry ketone; The phase C includes 0.5% cetearyl glucoside, 2.5% eicosyl glucoside, 0.3% behenyl alcohol, 2% dimethicone, and 0.5% sodium polyacryloyldimethyl taurate; The phase D includes 3% damask rose flower water, 1% p-hydroxyacetophenone, 1% 1,2-hexanediol, and 0.15% aminomethyl propanol.

6. The method for preparing a facial cream according to claim 4, characterized in that: The following steps are involved: (1) Add water to the phase A component and stir at 35-45 rpm for 15-25 min to swell and mix, then add the phase B component, heat to 75-85°C, and stir at 30-40 rpm until completely dissolved to obtain a phase AB mixture; (2) Stir the C phase components at 25-35 rpm for 30-40 min at a temperature of 75-85°C to mix them evenly; (3) Mix the AB phase mixture and the C phase components, homogenize at 2500-3000 rpm for 12-20 min, and then continue stirring at 25-35 rpm for 20-40 min to obtain an ABC phase mixture; (4) Stir the ABC phase mixture at 25-35 rpm and cool it to 40-50°C, add the D phase component, continue stirring to 22-26°C, degas, filter through 150-200 mesh, and obtain the facial cream.

7. Use of the skin barrier multiple repair composition according to claim 1 or 2 or the skin barrier multiple repair composition prepared by the preparation method according to claim 3 in the preparation of skin care products.

8. Use of the facial cream according to claim 4 or 5 or the facial cream prepared by the preparation method according to claim 6 in preparing a skin care product suitable for sensitive skin that has the functions of moisturizing, repairing, soothing, mildness and non-irritation.

Images