Anti-hair loss composition as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of hair care, and discloses an anti-hair-loss composition, a preparation method and application, the anti-hair-loss composition comprises the following components in percentage by weight: 70-99.9% of a mixture composed of a cacumen biotae extract, a ganoderma lucidum extract and a ginseng root extract, and 0.1-30% of an olive leaf extract; wherein the Chinese arborvitae twig and leaf extract, the ganoderma lucidum extract and the ginseng root extract are obtained by extracting Chinese arborvitae twig and leaf, ganoderma lucidum and ginseng root in an independent or composite extraction mode, and the weight ratio of the Chinese arborvitae twig and leaf to the ganoderma lucidum to the ginseng root is (1-9): (0.1-3): (0.1-3). Through compounding of the cacumen biotae extract, the ganoderma lucidum extract, the ginseng root extract and the olive leaf extract, the anti-hair loss effect of the composition is synergistically improved from the aspects of bacteriostasis, cell proliferation promotion, oxidation resistance and the like.

Description

Technical Field

[0001] The invention relates to the technical field of hair care, and in particular to an anti-hair loss composition, a preparation method and application thereof. Background Art

[0002] Current research has found that women often experience postpartum hair loss, which is a major problem faced by many new mothers. Postpartum hair loss is mainly caused by the following factors:

[0003] 1. Hair loss caused by changes in hormone levels: Women have higher estrogen levels during pregnancy, which drop sharply after giving birth, causing hair follicles to enter a resting phase, causing hair loss;

[0004] 2. Care and washing of hair during the postpartum recovery period needs to be handled with caution, resulting in a significant decrease in the frequency of hair care and washing. In addition, the oil secretion of the scalp of women during the postpartum recovery period is unbalanced, which leads to a decline in scalp health and hair loss;

[0005] 3. The mother is under tremendous physical and psychological pressure during the delivery process, which may have a negative impact on the nervous system and further affect the blood circulation of the scalp and the hair growth environment.

[0006] Prior art 1: Chinese patent application 201911426717.1 discloses a medicine for hair or scalp and a preparation method thereof. The medicine for hair or scalp is prepared from the following components in proportion by weight: 30-60 parts of Eclipta prostrata, 15-30 parts of Polygonum multiflorum, 25-50 parts of mulberry leaves, 10-35 parts of Platycladus orientalis leaves, 25-50 parts of Salvia miltiorrhiza, 10-55 parts of cedarwood alcohol, 2.5-5 parts of pumpkin seed oil, 1-5 parts of Artemisia argyi, 3-8 parts of Sophora flavescens, 1-4 parts of cloves, 1.5-4 parts of Daphne genkwa, and 2-8 parts of Schizonepeta tenuifolia. The preparation method of the medicine for hair or scalp comprises the following steps: S1: preparing medicinal solution a for standby use, S2: preparing medicinal solution b for standby use, S3: preparing mixed powder c, and S4: preparing the medicine.

[0007] Prior art 1 uses Chinese medicine such as Platycladus orientalis leaves to prepare medicine, which can kill head mites in deep layers and protect hair follicles, thereby achieving the effect of removing mites, controlling oil and promoting hair regeneration. It has the effects of growing and nourishing hair, strengthening hair and preventing hair loss, repairing scalp cells and hair follicles, replenishing pigments and controlling oil and removing dandruff simultaneously; and the Chinese medicine used in prior art 1 is not very irritating, which provides certain ideas for women's postpartum anti-hair loss and anti-hair loss care.

[0008] However, the prior art 1 directly uses Chinese medicine to make hair care products, which has the following defects:

[0009] (1) Chinese medicine raw materials contain other impurities, some of which may even have side effects on the hair or scalp;

[0010] (2) As the cell walls of the Chinese medicine raw materials have not been completely destroyed, the efficiency of the hair care ingredients in them in directly acting on the hair or scalp is low, resulting in insufficient anti-hair loss effect.

[0011] Based on the above defects, the problem of the prior art is that: no effective ingredients that directly act on the hair or scalp are effectively extracted to form an anti-hair loss composition, and the anti-hair loss effect is low. Summary of the invention

[0012] One of the purposes of the present invention is to provide an anti-hair loss composition, which is compounded with a variety of plant extracts, and can effectively act on the hair and scalp while synergistically improving the anti-hair loss effect.

[0013] Another object of the present invention is to provide a method for preparing an anti-hair loss composition, wherein the anti-hair loss composition prepared by the method can maximize the anti-hair loss efficacy of the composition.

[0014] Another object of the present invention is to provide a use of an anti-hair loss composition, which can be used to prepare daily-use cleaning and care products, especially daily-use cleaning products such as conditioners and shampoos, and can exert excellent anti-hair loss effects.

[0015] To achieve the above object, the present invention provides an anti-hair loss composition, which comprises, by weight percentage, a mixture of 70-99.9% of Platycladus orientalis leaf extract, Ganoderma lucidum extract, and ginseng root extract, and 0.1-30% of olive leaf extract;

[0016] The Platycladus orientalis leaf extract, Ganoderma lucidum extract and ginseng root extract are obtained by extracting Platycladus orientalis leaf, Ganoderma lucidum and ginseng root separately or in combination, and the weight ratio of Platycladus orientalis leaf, Ganoderma lucidum and ginseng root is 1-9:0.1-3:0.1-3.

[0017] The volatile oil contained in Platycladus orientalis leaf extract can prevent the spread of Staphylococcus aureus, remove dandruff and promote hair growth, and has a certain protective effect on the hair follicle damage caused by cyclophosphamide, slowing down the hair from entering the regression phase, thereby playing a role in preventing and treating hair loss; and the total flavonoids in Platycladus orientalis leaf extract can activate the stem cell characteristics of hair matrix cells to divide, proliferate and differentiate, and can restore vitality to some hair follicles.

[0018] Ganoderma lucidum, also known as Lingzhi, is a good medicine for nourishing and strengthening the body. It has the effects of replenishing qi and calming the mind, relieving cough and relieving asthma. It has a certain auxiliary therapeutic effect on postpartum hair loss; it has the effects of replenishing qi and nourishing blood, promoting blood circulation and removing blood stasis, and strengthening the stomach and calming the mind. Zhang Zhongjing believed in "Golden Chamber Synopsis" that hair is related to the heart, lungs, liver, kidneys, essence, qi, and blood. The heart governs blood vessels and consciousness, the lungs govern the hair, and the skin and fur are connected; the liver governs the storage of blood and the regulation of qi and blood; the kidneys store essence, and its beauty is in the hair. From the perspective of traditional Chinese medicine, postpartum hair loss may be caused by insufficient kidney essence and deficiency of qi and blood. Lingzhi has the effects of replenishing kidney, replenishing qi, and nourishing blood, and has a certain effect on postpartum hair loss caused by insufficient kidney essence and deficiency of qi and blood. Modern pharmacological studies have shown that Lingzhi has the effects of regulating the body's immune function, enhancing physical fitness, dilating blood vessels, promoting blood circulation, lowering blood lipids and calming.

[0019] Ginseng is known as the "king of herbs" as a tonic and health-care product. The main active ingredient of ginseng is ginsenoside, and its main medicinal value is to promote the differentiation of hair follicles, promote blood circulation, and resist inflammation and antibacterial effects. "Compendium of Materia Medica" records that eating ginseng after childbirth can replenish deficiency and promote blood circulation. Ginseng can expand the capillaries in the head and improve the nutritional status of hair; the effective ingredients of ginseng are absorbed by the hair, which can increase the tensile strength and elongation of the hair, making the hair smoother. Ginseng has the function of preventing and reducing hair loss and promoting hair growth and development, and the effect is significant; the mechanism of ginseng hair growth and anti-hair loss is:

[0020] 1) Ginseng contains plant hormones related to estrogen. The presence of these substances can improve blood circulation in the head skin and regulate immunity, thereby promoting hair growth.

[0021] 2) Reduce apoptosis and degeneration of keratinocytes in the growth phase of hair follicles.

[0022] Ginseng root extract can dilate the capillaries in the head, increase the nutritional supply of the hair, and improve the toughness of the hair, thereby reducing hair loss and hair breakage, and it also has a protective effect on damaged hair; Ginseng root extract contains ginsenosides and polysaccharides, ginsenosides are its main components, which are relatively easy to penetrate the skin surface and be absorbed by the dermis, and have a protective function for the skin and hair; Ginseng extract can also inhibit DKK-1-induced hair follicle degeneration by regulating the expression of Bcl-2 and Bax, thereby stimulating the proliferation of outer root sheath keratinocytes and reducing hair loss.

[0023] Olive leaf extract contains a variety of anti-inflammatory and antioxidant ingredients, such as oleuropein, hydroxytyrosol, etc., which can effectively avoid oxidative stress of the scalp and effectively slow down hair loss.

[0024] Preferably, the anti-hair loss composition also includes 0.001-2% 10-hydroxydecanoic acid; 10-hydroxydecanoic acid can effectively inhibit 5α-reductase.

[0025] Preferably, the anti-hair loss composition also includes 0.1-10% capryloyl glycine; capryloyl glycine can effectively adjust the pH environment of sebum and can effectively avoid the problem of hair damage caused by untimely hair care.

[0026] Preferably, the anti-hair loss composition also includes 0.1-40% polyglyceryl-10 laurate; polyglyceryl-10 laurate has a very strong cleaning effect and can effectively clean impurities such as debris on the hair and scalp.

[0027] The present invention also provides a method for preparing the above-mentioned anti-hair loss composition, and the anti-hair loss composition is obtained by mixing Platycladus orientalis leaf extract, Ganoderma lucidum extract, Panax ginseng root extract and Olea europaea leaf extract.

[0028] In the technical solution of the present invention, the Platycladus orientalis leaf extract, Ganoderma lucidum extract, Panax ginseng root extract and Olea europaea leaf extract can all be obtained through commercially available products.

[0029] Further preferably, the mixture of the Platycladus orientalis leaf extract, Ganoderma lucidum extract and ginseng root extract is obtained by extracting Platycladus orientalis leaf, Ganoderma lucidum and ginseng root through composite extraction.

[0030] Furthermore, the specific steps of the composite extraction include:

[0031] Step 1: adding Platycladus orientalis leaves into water for ultrasonic extraction to obtain a mixture;

[0032] Step 2: adding red ganoderma lucidum, ginseng root and water to the mixture for composite extraction, and then separating to obtain a filtrate;

[0033] Step 3: adding the filtrate into water for ultrasonic treatment to obtain a treated liquid;

[0034] Step 4: subjecting the treated liquid to ethanol precipitation for 12 to 14 hours to obtain an alcohol precipitation liquid, and then concentrating the solution to obtain an alcohol-free concentrated solution;

[0035] Step 5: adding an extractant to the alcohol-free concentrated solution to extract and obtain an extract;

[0036] Step 6: ultrafiltration of the extract to obtain the permeate to obtain Platycladus orientalis leaf extract, Ganoderma lucidum extract and Panax ginseng root extract;

[0037] In the step 1, the ultrasonic extraction temperature is 55-65° C., the ultrasonic power is 180 W, the ultrasonic time is 40-45 min, the weight ratio of Platycladus orientalis leaves to water is 1:1-3; the weight ratio of Platycladus orientalis leaves, Ganoderma lucidum, and ginseng roots is 1-9:0.1-3:0.1-3.

[0038] Preferably, the specific operation of step 2 is: adding red ganoderma, ginseng root and water to the mixture, extracting at 45-55°C for 2h and separating to obtain a first filtrate and a filter residue, then adding water to the filter residue, extracting at 45-55°C for 2h and separating to obtain a second filtrate, and mixing the first filtrate and the second filtrate to obtain a mixed filtrate;

[0039] In the step 2, the amount of water added in each extraction is 4 to 8 times the total weight of the Platycladus orientalis leaves, Ganoderma lucidum and ginseng roots.

[0040] In step 3, the weight ratio of the filtrate to water is 10:1, the ultrasonic power of the ultrasonic treatment is 180 W, and the ultrasonic time is 20 to 25 min.

[0041] Preferably, the specific operation of step 4 is: first use a single-effect concentrator to concentrate the treated liquid to 10%-20% of the original mass, then add an equal mass of 95% ethanol solution and let it stand for 12-14 hours, centrifuge, take the supernatant to obtain an alcohol precipitate, transfer the alcohol precipitate to a falling film concentrator, and vacuum concentrate the filtrate to zero ethanol content at a concentration temperature of 50-60° C. and a concentration pressure of -0.07 MPa to -0.09 MPa to obtain an alcohol-free concentrate.

[0042] Preferably, the extractant in step 5 is at least one of water, butylene glycol, glycerin and methylpropanediol, the amount of the extractant added is 1 to 5 times the total weight of Platycladus orientalis leaves, Ganoderma lucidum and ginseng roots, and the extraction time is 30 to 50 minutes.

[0043] Preferably, the specific operation of step 6 is: adding the extract to an ultrafiltration device loaded with an ultrafiltration membrane with a molecular weight of 150kDa to 500kDa for ultrafiltration to obtain a mixture of Platycladus orientalis leaf extract, Ganoderma lucidum extract and ginseng root extract, wherein the temperature during ultrafiltration is controlled within 35°C.

[0044] The invention also discloses a use of the anti-hair loss composition for preparing daily-use cleaning chemicals.

[0045] Beneficial Effects

[0046] Compared with the prior art, the present invention has at least the following advantages:

[0047] (1) The present invention improves the anti-hair loss effect of the composition in terms of antibacterial, cell proliferation promotion, and anti-oxidation by compounding Platycladus orientalis leaf extract, Ganoderma lucidum extract, ginseng root extract, and olive leaf extract;

[0048] (2) The present invention effectively improves the synergistic anti-hair loss effect of the composition by using the technical means of composite extraction of Platycladus orientalis leaves and olive leaves;

[0049] (3) The anti-hair loss composition of the present invention can effectively prevent hair loss and the shrinkage and closure of scalp hair follicles when used in daily cleaning and care products. In particular, it can effectively care for hair in the postpartum recovery period of women and avoid problems such as hair damage and hair loss caused by not washing hair for a long time during the recovery period. BRIEF DESCRIPTION OF THE DRAWINGS

[0050] The present invention is further described below in conjunction with the accompanying drawings and embodiments;

[0051] Figure 1 This is a chicken embryo diagram before the present invention is used in Application Example 1;

[0052] Figure 2 This is a picture of a chicken embryo after using Application Example 1 of the present invention. DETAILED DESCRIPTION

[0053] The present invention will be further described below in conjunction with the embodiments, but it does not constitute any limitation to the present invention. Any limited number of modifications made within the scope of the claims of the present invention are still within the scope of the claims of the present invention.

[0054] In order to explain the technical content of the present invention in detail, further description is given below in conjunction with the implementation modes.

[0055] In the following examples and comparative examples, the olive leaf extract was purchased from Shanghai Boshuo Industrial Co., Ltd., and the ginger root extract, Polygonum multiflorum extract, Angelica sinensis root extract, and Centella asiatica extract were purchased from Guangzhou Hemiao Biotechnology Co., Ltd.

[0056] It should be noted that the technical solution of the present invention can be implemented through other commercially available products and is not limited to the extracts provided by the above-mentioned manufacturers.

[0057] In the following examples and comparative examples, unless otherwise specified, % and parts are weight percentage and weight parts, respectively.

[0058] Example 1

[0059] An anti-hair loss composition, which is obtained by mixing 98.21% of a mixture of Platycladus orientalis leaf extract, Ganoderma lucidum extract, and Panax ginseng root extract with 1.79% of Olea europaea leaf extract;

[0060] The Platycladus orientalis leaf extract, Ganoderma lucidum extract and Ginseng root extract are respectively extracted from 20 parts of Platycladus orientalis leaves, 1 part of Ganoderma lucidum and 1 part of Ginseng root according to the following steps:

[0061] Step 1: crush the raw materials and sieve to obtain 100 mesh particles;

[0062] Step 2: adding water three times the weight of the particles to the particles obtained in step 1, extracting at 50° C. for 2 hours and separating to obtain a first filtrate and a filter residue, then adding water to the filter residue, extracting at 50° C. for 2 hours and separating to obtain a second filtrate, and mixing the first filtrate and the second filtrate to obtain a water-soluble extract of Platycladus orientalis leaves, Ganoderma lucidum or ginseng roots;

[0063] Step 3: subjecting the water-soluble extract to ethanol precipitation for 12 hours to obtain an alcohol extract, transferring the alcohol extract to a falling film concentrator, and concentrating the filtrate under vacuum at a concentration temperature of 55° C. and a concentration pressure of -0.08 MPa to zero ethanol content to obtain an alcohol-free concentrate;

[0064] Step 4: adding an extractant to the alcohol-free concentrate and extracting for 30 minutes to obtain an extract;

[0065] Step 5: Add the extract to an ultrafiltration device loaded with an ultrafiltration membrane with a molecular weight of 300 kDa for ultrafiltration to obtain the permeate to obtain Platycladus orientalis leaf extract, Ganoderma lucidum extract or ginseng root extract, wherein the temperature during ultrafiltration is controlled within 35°C.

[0066] Example 2

[0067] An anti-hair loss composition, which is obtained by mixing 98.21% of a mixture of Platycladus orientalis leaf extract, Ganoderma lucidum extract, and Panax ginseng root extract with 1.79% of Olea europaea leaf extract;

[0068] The mixture of the Platycladus orientalis leaf extract, Ganoderma lucidum extract and Ginseng root extract is obtained by composite extraction of Platycladus orientalis leaf, Ganoderma lucidum and Ginseng root, wherein the weight ratio of Platycladus orientalis leaf, Ganoderma lucidum and Ginseng root is 20:1:1, and the specific steps of the composite extraction include:

[0069] Step 1: Crush and sieve the Platycladus orientalis leaves, Ganoderma lucidum and Ginseng roots into 100-mesh granules.

[0070] Step 2: adding the Platycladus orientalis leaf particles into water 3 times the weight of the Platycladus orientalis leaf particles, and performing ultrasonic extraction at an ultrasonic extraction temperature of 60° C. and an ultrasonic power of 180 W for 40 min to obtain a mixture;

[0071] Step 3: add water of 5 times the total weight of Platycladus orientalis leaf particles, red ganoderma granules, and ginseng root particles to the mixture, extract at 45-55° C. for 2 hours, and separate to obtain a first filtrate and a filter residue, then add water of 5 times the total weight of Platycladus orientalis leaf particles, red ganoderma granules, and ginseng root particles to the filter residue, extract at 45-55° C. for 2 hours, and separate to obtain a second filtrate, and mix the first filtrate and the second filtrate to obtain a mixed filtrate;

[0072] Step 4: adding the filtrate to water for ultrasonic treatment at room temperature to obtain a treated liquid, wherein the weight ratio of the filtrate to water is 10:1, the ultrasonic power of the ultrasonic treatment is 180 W, and the ultrasonic time is 20 min;

[0073] Step 5: first use a single-effect concentrator to concentrate the treated liquid to 15% of the original mass, then add an equal mass of 95% ethanol solution and let it stand for 12 hours, centrifuge, take the supernatant to obtain an alcohol precipitate, transfer the alcohol precipitate to a falling film concentrator, and vacuum concentrate the filtrate at a concentration temperature of 60° C. and a concentration pressure of -0.08 MPa to zero ethanol content to obtain an alcohol-free concentrate;

[0074] Step 6: adding water to the alcohol-free concentrate to extract to obtain an extract, wherein the amount of water is 3 times the total weight of the Platycladus orientalis leaves, Ganoderma lucidum, and ginseng roots, and the extraction time is 30 minutes;

[0075] Step 7: Add the extract to an ultrafiltration device loaded with an ultrafiltration membrane with a molecular weight of 300 kDa for ultrafiltration to obtain the composite extracted Platycladus orientalis leaf extract, Ganoderma lucidum extract and ginseng root extract, wherein the temperature during ultrafiltration is controlled within 35°C.

[0076] Example 3

[0077] The method is substantially the same as Example 2, except that the anti-hair loss composition is obtained by mixing 96.5% of a mixture of Platycladus orientalis leaf extract, Ganoderma lucidum extract, and Panax ginseng root extract with 3.5% of Olea europaea leaf extract.

[0078] Example 4

[0079] The method is substantially the same as Example 2, except that the anti-hair loss composition is obtained by mixing 97.2% of a mixture of Platycladus orientalis leaf extract, Ganoderma lucidum extract, and Panax ginseng root extract, 1.8% of Olea europaea leaf extract, and 1% of 10-hydroxydecanoic acid.

[0080] Example 5

[0081] The method is substantially the same as Example 2, except that the anti-hair loss composition is obtained by mixing 88.4% of a mixture of Platycladus orientalis leaf extract, Ganoderma lucidum extract, and Panax ginseng root extract, 1.6% of Olea europaea leaf extract, and 10% of capryloyl glycine.

[0082] Example 6

[0083] The method is substantially the same as Example 2, except that the anti-hair loss composition is obtained by mixing 88.4% of a mixture of Platycladus orientalis leaf extract, Ganoderma lucidum extract, and Panax ginseng root extract, 1.6% of Olea europaea leaf extract, and 10% of polyglycerol-10 laurate.

[0084] Example 7

[0085] It is generally the same as Example 2, except that the anti-hair loss composition is obtained by mixing 62% of a mixture of Platycladus orientalis leaf extract, Ganoderma lucidum extract, and ginseng root extract, 3% of olive leaf extract, 0.08% of 10-hydroxydecanoic acid, 6% of capryloyl glycine, and 28.92% of polyglycerol-10 laurate.

[0086] Comparative Example 1

[0087] It is substantially the same as Example 2, except that the anti-hair loss composition is obtained by mixing a mixture of 83.4% of Ganoderma lucidum extract and ginseng root extract and 16.6% of olive leaf extract; wherein the mixture of Ganoderma lucidum extract and ginseng root extract is extracted by the composite extraction method of Example 2, and the weight ratio of Ganoderma lucidum to ginseng root is 1:1.

[0088] Comparative Example 2

[0089] It is generally the same as Example 2, except that the anti-hair loss composition is obtained by mixing a mixture of 98.1% of Platycladus orientalis leaf extract and ginseng root extract and 1.9% of olive leaf extract; wherein the mixture of Platycladus orientalis leaf extract and ginseng root extract is extracted by the composite extraction method of Example 2, and the weight ratio of Platycladus orientalis leaf to ginseng root is 20:1.

[0090] Comparative Example 3

[0091] It is generally the same as Example 2, except that the anti-hair loss composition is obtained by mixing a mixture of 98.1% of Platycladus orientalis leaf extract and Ganoderma lucidum extract and 1.9% of olive leaf extract; wherein the mixture of Platycladus orientalis leaf extract and Ganoderma lucidum root extract is extracted by the composite extraction method of Example 2, and the weight ratio of Platycladus orientalis leaf to Ganoderma lucidum is 20:1.

[0092] Comparative Example 4

[0093] The method is substantially the same as Example 2, except that the anti-hair loss composition is 100% Platycladus orientalis leaf extract, Ganoderma lucidum extract, and Panax ginseng root extract.

[0094] Comparative Example 5

[0095] It is substantially the same as Example 2, except that the anti-hair loss composition is obtained by mixing a mixture of 98.21% ginger root extract, red ganoderma lucidum extract, and ginseng root extract and 1.79% olive leaf extract; wherein the mixture of ginger root extract, red ganoderma lucidum root extract, and ginseng root extract is extracted by the composite extraction method of Example 2, and the weight ratio of the ginger root, red ganoderma lucidum, and ginseng root is 20:1:1.

[0096] Comparative Example 6

[0097] It is generally the same as Example 2, except that the anti-hair loss composition is obtained by mixing 98.21% of a mixture of Platycladus orientalis leaf extract, Polygonum multiflorum extract, and ginseng root extract and 1.79% of olive leaf extract; wherein the mixture of Platycladus orientalis leaf extract, Polygonum multiflorum extract, and ginseng root extract is extracted by the composite extraction method of Example 2, and the weight ratio of the Platycladus orientalis leaf, Polygonum multiflorum, and ginseng root is 20:1:1.

[0098] Comparative Example 7

[0099] It is generally the same as Example 2, except that the anti-hair loss composition is obtained by mixing a mixture of 98.21% of Platycladus orientalis leaf extract, Ganoderma lucidum extract, and Angelica sinensis extract and 1.79% of Olea europaea leaf extract; wherein the mixture of Platycladus orientalis leaf extract, Ganoderma lucidum extract, and Angelica sinensis extract is extracted by the composite extraction method of Example 2, and the weight ratio of Platycladus orientalis leaf, Ganoderma lucidum, and Angelica sinensis is 20:1:1.

[0100] Comparative Example 8

[0101] The method is substantially the same as Example 2, except that the anti-hair loss composition is obtained by mixing 98.21% of a mixture of Platycladus orientalis leaf extract, Ganoderma lucidum extract, and Panax ginseng root extract and 1.79% of Centella asiatica extract.

[0102] Performance Testing

[0103] 5α-reductase inhibition rate

[0104] 1. Purpose and principle of the experiment

[0105] The secretion function of sebaceous glands in oily skin is relatively strong, the face feels greasy, difficult to clean, and has an oily sheen that affects the appearance; at the same time, it is also prone to some corresponding skin diseases, such as acne vulgaris and seborrheic dermatitis. In view of the relationship between 5α-reductase and sebaceous glands, the inhibitory effect of the test sample on 5α-reductase is usually tested to reflect the sebum regulation effect, so as to characterize the oil control effect of the test sample.

[0106] This test refers to the laboratory method (HMC-WI-031 5α-reductase inhibition rate) and compares the 5α-reductase inhibition rate test results of the test sample with that of the negative control. If the inhibition rate of the test sample is higher than that of the negative control and there is a significant difference (P<0.05), it can be considered that the test sample has a certain oil control effect.

[0107] This test method is an in vitro method and is suitable for evaluating cosmetic products that claim to achieve oil control by inhibiting 5α-reductase.

[0108] 2 Test indicators

[0109] Table 1 Test indicators

[0110]

[0111] 3 Experimental materials and methods

[0112] 3.1 Instruments and Equipment

[0113] BSA224S Analytical Balance

[0114] Agilent 1220 HPLC

[0115] 3.2 Reagents

[0116] Testosterone, 98%

[0117] NADPH, 90%

[0118] Anhydrous ethanol

[0119] 3.3 Test methods

[0120] (1) Treatment of control and test samples

[0121] Sample group: Examples 1-7 and Comparative Examples 1-8 were diluted with pure water to a sample concentration of 10%;

[0122] Positive control (finasteride, purity ≥98%): dilute with anhydrous ethanol to a positive control concentration of 0.1%;

[0123] Negative control: PBS solution.

[0124] (2) Test operation steps

[0125] A sample group, a positive control group, a negative control group and a blank group were set up. Three parallels were set up for each group. Different reagent solutions were added to the four groups respectively, and the solutions were shaken well. Each group was subjected to HPLC analysis to determine the testosterone content in each tube.

[0126] (3) Calculation formula

[0127]

[0128] (4) Data analysis

[0129] SPSS was used as the statistical analysis software, and the independent sample t-test was used to compare the 5α-reductase inhibition rates among the test samples, positive controls, and negative controls.

[0130] The above statistical analyses were all two-tailed tests, with a significance level of α = 0.05. P>0.05 means there is no significant difference between the two groups; P<0.05 means there is a significant difference between the two groups.

[0131] The anti-shedding compositions prepared in Examples 1-7 and Comparative Examples 1-8 were tested according to the above test method, and the results are shown in Table 2;

[0132] Table 2 5α-reductase inhibition rate of the anti-hair loss composition obtained in Examples 1-7 and Comparative Examples 1-8

[0133]

[0134]

[0135] According to the results in Table 2, we can see that:

[0136] According to the data of Examples 1-7, the compound of Platycladus orientalis leaf extract, Ganoderma lucidum extract, Panax ginseng root extract and Olea europaea leaf extract in the present invention can exert a significant 5α-reductase inhibitory effect;

[0137] A comparison of the data in Example 1 and Example 2 shows that there is a significant difference in the 5α-reductase inhibition rate that can be exerted by the single extraction and the combined extraction of Platycladus orientalis leaves, Ganoderma lucidum, and ginseng roots. It is preliminarily speculated that this is because there is a synergistic solubilization effect between the effective ingredients when Platycladus orientalis leaves, Ganoderma lucidum, and ginseng roots are combined, thereby increasing the content of the effective ingredients of the entire composite extract, and effectively improving the 5α-reductase inhibition effect of the anti-hair loss composition.

[0138] According to the data comparison between Examples 4-6 and Example 2, since 10-hydroxydecanoic acid, capryloyl glycine, and polyglycerol-10 laurate have different effects, the 5α-reductase inhibition rates of Examples 4-6 are also different under the same total dosage. The addition of 10-hydroxydecanoic acid in Example 4 improves the 5α-reductase inhibition rate of the composition to a certain extent. Since the dosage of other components is reduced in Examples 5 and 6, and capryloyl glycine and polyglycerol-10 laurate do not have a high inhibitory effect on 5α-reductase, the inhibitory effect of 5α-reductase of the composition is reduced. However, in Example 7, 10-hydroxydecanoic acid and capryloyl glycine are used to inhibit the 5α-reductase of the composition. The technical solution in which 10-hydroxydecanoic acid, capryloyl glycine and polyglycerol-10 laurate are added to Platycladus orientalis leaf extract, Ganoderma lucidum extract, ginseng root extract and olive leaf extract, and the 5α-reductase inhibitory effect thereof is greatly improved compared with that of Example 2. It is speculated that the compounding of 10-hydroxydecanoic acid, capryloyl glycine and polyglycerol-10 laurate can synergistically enhance the 5α-reductase inhibitory effect of the compound composition of Platycladus orientalis leaf extract, Ganoderma lucidum extract, ginseng root extract and olive leaf extract. It may be that the compounding of 10-hydroxydecanoic acid, capryloyl glycine and polyglycerol-10 laurate can comprehensively maintain and clean the scalp, thereby exerting a stronger 5α-reductase inhibitory effect of the compound composition.

[0139] According to the data comparison of Example 2 and Comparative Examples 1-4, it can be seen that in the absence of any one of the Platycladus orientalis leaf extract, Ganoderma lucidum extract, ginseng root extract and olive leaf extract, the 5α-reductase inhibition rate of the composition is significantly reduced, indicating that the synergistic effect of the composition of the present invention in enhancing the 5α-reductase inhibition efficacy is based on the combination of Platycladus orientalis leaf extract, Ganoderma lucidum extract, ginseng root extract and olive leaf extract, and none of them can be missing.

[0140] According to the data comparison between Example 2 and Comparative Examples 5-8, although the technical solution of using ginger root extract to replace Platycladus orientalis leaf extract, Polygonum multiflorum extract to replace Ganoderma lucidum extract, Angelica sinensis root extract to replace Ginseng root extract, and Centella asiatica extract to replace Olive leaf extract can also produce a good 5α-reductase inhibition effect, it is significantly lower than that of Example 2, indicating that the composition of the present invention is a synergistic combination of Platycladus orientalis leaf extract, Ganoderma lucidum extract, Ginseng root extract and Olive leaf extract.

[0141] Application Example 1

[0142] An anti-hair loss essence, the formula ratio is shown in Table 3.

[0143] Table 3 Formula of anti-hair loss essence

[0144] Raw material name Addition amount wt / % The anti-shedding composition of Example 2 2.24 10-Hydroxydecanoic acid 0.045 Capryloyl Glycine 0.11 Polyglyceryl-10 Laurate 0.101 Disodium EDTA 0.05 Dipotassium glycyrrhizinate 0.15 Butanediol 5 Betaine 3 Sodium polyacryloyldimethyl taurate 0.5 p-Hydroxyacetophenone 0.4 1,2-Hexanediol 0.4 water margin

[0145] Application Example 2

[0146] An anti-hair loss shampoo, the formula ratio is shown in Table 4.

[0147] Table 4 Formula of anti-hair loss shampoo

[0148]

[0149]

[0150] Application Example 3

[0151] An anti-hair loss shampoo, the formula ratio is shown in Table 5.

[0152] Table 5: Formula of anti-hair loss shampoo

[0153]

[0154]

[0155] Safety Testing

[0156] Test method: Chicken embryo chorioallantoic membrane test

[0157] 1. Purpose and principle of the test

[0158] The chicken embryo chorioallantoic membrane test is an early in vitro eye irritation assessment method. The chorioallantoic membrane (CAM) is a respiratory membrane that surrounds the chicken embryo. This test uses the characteristics of the intact, clear and transparent vascular system of the chorioallantoic membrane of the mid-stage incubated chicken embryo. A certain amount of the test substance is directly exposed to the chicken embryo chorioallantoic membrane. After a period of action, the changes in the toxic effect indicators of the chorioallantoic membrane (such as bleeding, coagulation and vascular melting) are observed. These indicators reflect the changes in the morphological structure, color and permeability of the blood vessels and vascular networks, as well as the phenomena such as the denaturation of the chorioallantoic membrane proteins and the degree of damage. Then, a score is obtained by combining them to evaluate the eye irritation of the test substance.

[0159] The purpose of this test is to test the ability of the test substance to cause toxic changes in the chicken embryo chorioallantoic membrane and to evaluate the elements and processes of the potential eye irritation of the evaluated substance.

[0160] 2. Experimental Group Treatment and Reagents

[0161] Sample group: Application Examples 1-3 were diluted into 2% aqueous solution respectively.

[0162] Negative control: 0.9% saline.

[0163] Positive control: 1% sodium dodecyl sulfate (SDS) solution.

[0164] 3. Experimental steps

[0165] In this test, 6 chicken embryos were selected for each group, and the conditions of the chorioallantoic membrane were recorded with a camera. A polytetrafluoroethylene resin ring was placed on the chorioallantoic membrane of the chicken embryo and photographed. The sample to be tested was added to the polytetrafluoroethylene resin ring, the time of adding the sample was recorded, and the air chamber was covered with a moistened plastic wrap. The chicken embryo was moved to a constant temperature and humidity chamber for culture, and the degree of change of each toxic effect was observed.

[0166] 4. Calculation of results

[0167] The endpoint evaluation method was used to perform the test and the endpoint evaluation (ES) was calculated with two decimal places. Each chicken embryo was scored as the sum of the bleeding, coagulation and vascular melting observed in each chicken embryo. The average value of the mathematical sum obtained for ES-6 chicken embryos was taken.

[0168] The average ES score was calculated according to the following formula.

[0169]

[0170] 5. Result judgment criteria

[0171] ES≤4, non-irritating;

[0172] 4<ES≤12, mild irritation;

[0173] 12<ES<16, moderate irritation;

[0174] ES ≥ 16, strong irritant / corrosive.

[0175] 2. Test Results

[0176] According to the above test method, application examples 1-3 were tested by sample sending, and the ES were all less than 4, and the results were all judged to be non-irritating.

[0177] Among them, the results of chicken embryos before and after use in Application Example 1 are as follows Figure 1-Figure 2 As shown, the sample test result of Application Example 1 is ES=2.33;

[0178] This proves that the technical solution of the present invention is mild and non-irritating.

[0179] The embodiments presented herein are only embodiments selected according to the combination of all possible embodiments. The appended claims should not be limited by the embodiments describing the present invention. Some numerical ranges used in the claims include sub-ranges therein, and the changes in these ranges should also be covered by the appended claims.

Claims

1. An anti-shedding composition, characterized in that The invention comprises, by weight percentage, a mixture of 70 to 99.9% of Platycladus orientalis leaf extract, Ganoderma lucidum extract, and Panax ginseng root extract and 0.1 to 30% of Olea europaea leaf extract; The Platycladus orientalis leaf extract, Ganoderma lucidum extract and Ginseng root extract are obtained by extracting Platycladus orientalis leaf, Ganoderma lucidum and Ginseng root separately or in combination, wherein the weight ratio of Platycladus orientalis leaf, Ganoderma lucidum and Ginseng root is 1 to 9: 0.1～3：0.1～3。 2. The anti-shedding composition according to claim 1, characterized in that Also included is 0.001 to 2% 10-hydroxydecanoic acid.

3. The anti-shedding composition according to claim 1, characterized in that Also included is 0.1-10% capryloyl glycine.

4. The anti-shedding composition according to claim 1, characterized in that It also includes 0.1-40% polyglyceryl-10 laurate.

5. A method for preparing the anti-shedding composition according to claim 1, characterized in that: The anti-hair loss composition is obtained by mixing Platycladus orientalis leaf extract, Ganoderma lucidum extract, Panax ginseng root extract and Olea europaea leaf extract.

6. Use of the anti-hair loss composition according to any one of claims 1 to 4 in preparing daily-use cleaning products.

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