Sugarcane polyphenol composition with anti-aging effect and application thereof
Through the development of sugarcane polyphenol compositions, the side effects and inefficiency of existing anti-aging drugs have been solved, significantly extending the lifespan of C. elegans and improving their antioxidant stress capabilities, demonstrating its potential in the field of anti-aging.
Patent Information
- Application Number
- CN202411364006.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-28
- Publication Date
- 2024-12-17
AI Technical Summary
Existing anti-aging drugs have potential side effects and are difficult to effectively delay the aging process, resulting in the occurrence of senile diseases and reduced quality of life.
Develop a sugarcane polyphenol composition including gallic acid, quercetin, catechol, rutin and epigalactate catechin, which is prepared by mixing and ultrafine crushing for extended life and improved antioxidant Stress ability.
It significantly extends the average lifespan of C. elegans and improves the antioxidant stress ability of nematodes, has significant anti-aging and prolonging lifespan effects, reducing the impact of oxidative stress.
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Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of biomedicine, and in particular relates to a sugarcane polyphenol composition with anti-aging effect and application thereof. Background Art
[0002] As a complex biological process, aging is not only reflected in the gradual decline of physiological functions, but also in the functional aging of cells, tissues and even the whole organism. Genetic factors, as an intrinsic driver, determine the framework of the life cycle of an organism by regulating the expression pattern of specific genes. Environmental factors, especially eating habits, lifestyle, and the physical and chemical environment to which they are exposed, further characterize the trajectory of aging, accelerating or slowing down the process. With age, the metabolic efficiency of organisms declines significantly, which is mainly attributed to the reduction of mitochondrial energy production in cells and the reduced efficiency of repair mechanisms. This is followed by the structural and functional degeneration of tissues and organs, which lose their original elasticity and regenerative ability. In addition, aging is accompanied by an increase in oxidative stress levels, and the accumulation of free radicals leads to cell damage; the persistence of chronic low-grade inflammation exacerbates tissue damage; mitochondrial dysfunction further inhibits energy output. These changes together lead to the occurrence of a variety of geriatric diseases. Neurodegenerative diseases such as diabetes, Alzheimer's disease, Parkinson's disease, as well as cardiovascular diseases and various cancers have become the most common health threats in the elderly population. These diseases not only seriously reduce the quality of life of the elderly, but also put great pressure on the public health system and consume a large amount of medical resources.
[0003] In view of this, the scientific research community and the pharmaceutical industry are actively exploring safe and effective anti-aging strategies. Although existing anti-aging drugs are mostly chemically synthesized products, they can intervene in the aging process to a certain extent, but their potential side effects cannot be ignored, prompting scientists to turn to nature for answers. Natural active substances, such as plant extracts, microbial metabolites, etc., have shown great potential in delaying aging and improving the quality of life due to their diverse biological activities and relatively low toxicity. Therefore, in-depth research and rational development of these natural resources can not only enrich the means of anti-aging treatment, but also reduce the socioeconomic burden caused by senile diseases. The development and application of natural active substances with anti-aging and life-extending effects have broad prospects. Summary of the invention
[0004] In order to solve the above technical problems, the present invention proposes a sugarcane polyphenol composition with anti-aging effect and its application. The sugarcane polyphenol composition provided by the present invention can significantly prolong the average lifespan of Caenorhabditis elegans under natural conditions, improve the nematode's ability to resist oxidative stress, and has significant anti-aging and life-extending effects.
[0005] The present invention provides the following technical solutions:
[0006] Technical solution 1: A sugarcane polyphenol composition with anti-aging effect, including gallic acid, quercetin, catechol, rutin and epigallocatechin gallate.
[0007] Furthermore, based on weight parts, it includes 5-10 parts of gallic acid, 3-9 parts of quercetin, 3-6 parts of catechol, 2-5 parts of rutin and 2-5 parts of epigallocatechin gallate.
[0008] Furthermore, based on weight parts, it includes 8.9 parts of gallic acid, 5.6 parts of quercetin, 4.2 parts of catechol, 3.4 parts of rutin and 2.9 parts of epigallocatechin gallate.
[0009] Technical Solution 2: A method for preparing the sugarcane polyphenol composition, comprising the following steps: fully mixing gallic acid, quercetin, catechol, rutin and epigallocatechin gallate to obtain a mixture, and then ultrafine grinding the mixture, passing it through a sieve to obtain a sugarcane polyphenol composition in the form of fine powder.
[0010] Furthermore, the pore size of the sieve is 0.5 mm.
[0011] Technical solution three: Use of the sugarcane polyphenol composition in the preparation of anti-aging products.
[0012] Furthermore, the anti-aging approach includes extending lifespan and improving the ability to resist oxidative stress.
[0013] Technical Solution 4: An anti-aging product, the active ingredients of which include the sugarcane polyphenol composition.
[0014] Compared with the prior art, the present invention has the following advantages and technical effects:
[0015] Caenorhabditis elegans is a small, non-toxic and harmless nematode that can survive independently. It has the advantages of a short life cycle, easy observation of the worm body, clear genetic background, and completed genome sequencing. It is an ideal biological model for studying aging and lifespan. The present invention uses Caenorhabditis elegans as a model organism for anti-aging research, and conducts experiments on an aging model and an oxidative stress model at the level of whole animals. The results show that the sugarcane polyphenol composition provided by the present invention can significantly prolong the average lifespan of Caenorhabditis elegans under natural conditions, improve the nematode's ability to resist oxidative stress, and has significant anti-aging and lifespan-extending effects. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.
[0017] Figure 1 The effect of each treatment group on the life span of Caenorhabditis elegans under natural aging conditions;
[0018] Figure 2 is the effect of each treatment group on the survival rate of Caenorhabditis elegans under oxidative stress;
[0019] Figure 3 The effect of each treatment group on the ROS content in Caenorhabditis elegans under oxidative stress;
[0020] Figure 4 The effects of each treatment group on the SOD content in Caenorhabditis elegans under oxidative stress:
[0021] Figure 5 The effects of each treatment group on the CAT content in Caenorhabditis elegans under oxidative stress:
[0022] Figure 6 This is the effect of each treatment group on the MDA content in Caenorhabditis elegans under oxidative stress. DETAILED DESCRIPTION
[0023] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.
[0024] It should be understood that the terms described in the present invention are only for describing special embodiments and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.
[0025] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.
[0026] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.
[0027] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.
[0028] The experimental methods used in the examples of the present invention are all conventional methods unless otherwise specified; the reagents used are all commercially available materials, including gallic acid, quercetin, catechol, rutin and epigallocatechin-3-ol, unless otherwise specified.
[0029] In the embodiments of the present invention, sugarcane molasses was provided by Zhuhai Sino-US Pulai Health Technology Co., Ltd.; ultrafine grinder (Tianjin Test Instrument Co., Ltd.); reactive oxygen species (ROS), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA) assay kit (Nanjing Jiancheng Bioengineering Institute), gallic acid, quercetin, catechol, rutin and epigallocatechin gallate were derived from Sigma Chemical Company, USA.
[0030] Preparation of S Basal solution: weigh 2.93g NaCl, 3g KH2PO4, 0.66g K2HPO4·3H2O, add 500mL deionized water, sterilize by high pressure after complete dissolution, add 0.5mL cholesterol solution after cooling, and set aside after full dissolution.
[0031] Preparation of S Medium solution: Add sterilized CaCl2 solution (1 mol / L, 1.5 mL), MgSO4 solution (1 mol / L, 1.5 mL), potassium citrate solution (pH = 6.0, 1 mol / L, 5 mL) and trace element solution (5 mL) to S Basal solution, mix well and set aside.
[0032] Example 1
[0033] Preparation of sugarcane polyphenol composition: 8.9 g of gallic acid, 5.6 g of quercetin, 4.2 g of catechol, 3.4 g of rutin and 2.9 g of epigallocatechin gallate were mixed in a sealed bag, shaken thoroughly to mix, and then the mixture was ultrafinely ground and passed through a 0.5 mm sieve to obtain a composition in the form of fine powder.
[0034] Example 2
[0035] Preparation of sugarcane polyphenol composition: 5 g of gallic acid, 3 g of quercetin, 3 g of catechol, 2 g of rutin and 2 g of epigallocatechin gallate were mixed in a sealed bag, and the mixture was thoroughly shaken and mixed. Then, the mixture was ultrafinely ground and passed through a 0.5 mm sieve to obtain a composition in the form of fine powder.
[0036] Example 3
[0037] Preparation of sugarcane polyphenol composition: 10 g of gallic acid, 9 g of quercetin, 6 g of catechol, 5 g of rutin and 5 g of epigallocatechin gallate were mixed in a sealed bag, and the mixture was thoroughly shaken and mixed, and then the mixture was ultrafinely ground and passed through a 0.5 mm sieve to obtain a composition in the form of fine powder.
[0038] Comparative Example 1
[0039] Sugarcane molasses was used as the raw material. Sugarcane molasses (20 g) was dissolved in 380 mL of acidified water (adjusted to pH 5 with 1 mol / L hydrochloric acid), stirred magnetically at room temperature until uniformly dissolved, and pretreated Amberlite XAD-2 resin (100 g) was added. Static adsorption was performed at 30°C and 150 r / min for 12 h, and then the supernatant was separated from the resin. 400 mL of ethanol (50%, V / V) was added to the resin, and static adsorption was performed at 30°C and 150 r / min for 12 h. The supernatant was taken and freeze-dried after rotary evaporation to obtain the sugarcane polyphenol extract. The extract was then ultrafinely ground and passed through a 0.5 mm sieve to obtain an extract in the form of fine powder. The polyphenol composition in the extract was identified by high performance liquid phase, and the results are shown in Table 1.
[0040] Table 1
[0041] Phenolic monomers content / % gallic acid 8.59 Isoschaftoside 7.03 Schaftoside 5.70 5-Hydroxymethyl-2-furfural 5.38 Quercetin 5.16 Catechol 3.84 Coumarin 3.28 Rutin 3.00 1-Galloylglucose 2.91 Epigallocatechin gallate 2.73
[0042] Comparative Example 2
[0043] 4 g of gallic acid, 2.5 g of quercetin, 2.6 g of catechol, 1.5 g of rutin and 1.6 g of epigallocatechin gallate were mixed in a sealed bag, shaken thoroughly to mix, and then the mixture was ultrafinely ground and passed through a 0.5 mm sieve to obtain a composition in the form of fine powder.
[0044] Comparative Example 3
[0045] 11 g of gallic acid, 10 g of quercetin, 7 g of catechol, 5.5 g of rutin and 5 g of epigallocatechin gallate were mixed in a sealed bag, and the mixture was thoroughly shaken and then ultrafinely ground and passed through a 0.5 mm sieve to obtain a composition in the form of fine powder.
[0046] Comparative Example 4
[0047] Put 25 g of gallic acid in a sealed bag, shake it thoroughly to mix, and then grind it into ultrafine powder and pass it through a 0.5 mm sieve to obtain gallic acid in the form of fine powder.
[0048] Comparative Example 5
[0049] 25 g of quercetin was placed in a sealed bag, shaken and mixed thoroughly, and then ultrafine ground and passed through a 0.5 mm sieve to obtain quercetin in fine powder form.
[0050] Comparative Example 6
[0051] 25 g of catechol was placed in a sealed bag, shaken and mixed thoroughly, and then ultrafine ground and passed through a 0.5 mm sieve to obtain catechol in fine powder form.
[0052] Comparative Example 7
[0053] 25 g of rutin was placed in a sealed bag, shaken and mixed thoroughly, and then ultrafine ground and passed through a 0.5 mm sieve to obtain rutin in fine powder form.
[0054] Comparative Example 8
[0055] 25 g of gallic acid catechin was placed in a sealed bag, shaken and mixed thoroughly, and then ultrafine ground and passed through a 0.5 mm sieve to obtain gallic acid catechin in the form of fine powder.
[0056] Comparative Example 9
[0057] 15 g of gallic acid and 10 g of catechol were placed in a sealed bag, and the mixture was thoroughly shaken and mixed. The mixture was then ultrafinely ground and passed through a 0.5 mm sieve to obtain a composition in the form of fine powder.
[0058] Comparative Example 10
[0059] 10 g of gallic acid, 7.5 g of quercetin and 7.5 g of catechol were placed in a sealed bag and shaken thoroughly to mix. The mixture was then ultrafinely ground and passed through a 0.5 mm sieve to obtain a composition in the form of fine powder.
[0060] Experimental Example 1
[0061] Effects of single substances or compositions in fine powder form prepared in the treatment groups of Examples 1-3 and Comparative Examples 1-10 on the lifespan of Caenorhabditis elegans under natural aging conditions
[0062] The compositions in the form of fine powders of Examples 1-3 and Comparative Examples 1-10 were added to the synchronized L4-stage N2 wild-type Caenorhabditis elegans at a final concentration of 10 μg / mL, and an equal volume of S Medium solution was added to the control group. The three groups were repeated and cultured at 20°C. The survival of the nematodes was counted every 3 days until all the nematodes died. The lifespan of Caenorhabditis elegans was represented by a survival curve, and the results were represented by the Kaplan-Meier method.
[0063] The statistical results are as follows Figure 1 As shown in Table 2, the results show that the compositions in the form of fine powder prepared in Examples 1-3 can prolong the life span of nematodes under natural aging conditions, indicating that the sugarcane polyphenol composition of the present invention has good anti-aging activity.
[0064] Table 2
[0065] Group Average life span (d) Example 1 28.42 Example 2 26.02 Example 3 27.62 Comparative Example 1 24.12 Comparative Example 2 24.22 Comparative Example 3 23.59 Comparative Example 4 22.88 Comparative Example 5 21.67 Comparative Example 6 20.77 Comparative Example 7 20.74 Comparative Example 8 21.39 Comparative Example 9 22.94 Comparative Example 10 23.46 Comparison 18.56
[0066] Experimental Example 2
[0067] Effects of the single substance in fine powder form or the combination in fine powder form prepared in the treatment groups of Examples 1-3 and Comparative Examples 1-10 on the survival rate of Caenorhabditis elegans under oxidative stress
[0068] The fine powdered compositions in Examples 1-3 and Comparative Examples 1-10 with a final concentration of 10 μg / mL were added to the synchronized L4 stage N2 wild-type Caenorhabditis elegans, and an equal volume of S Medium solution was added to the control group, and repeated for 3 groups. After culturing at 20°C for 48 hours, paraquat with a final concentration of 70 mmol / L was added to model the oxidative stress of the nematodes (paraquat is a strong oxidant, which will induce the production of a large number of reactive oxygen free radicals after entering the body, causing oxidative stress, thereby accelerating the aging and death process of the body), and the survival of the nematodes was counted every 12 hours until all the nematodes died. The survival rate of Caenorhabditis elegans was expressed as a survival curve, and the results were expressed by the Kaplan-Meier method.
[0069] The statistical results are as follows Figure 2 As shown in Table 3, the results show that the compositions in the form of fine powder prepared in Examples 1-3 can prolong the survival life of nematodes under oxidative stress conditions, indicating that the sugarcane polyphenol composition of the present invention has good antioxidant activity.
[0070] Table 3
[0071] Group Average lifespan (h) Example 1 161.4 Example 2 147.5 Example 3 150.6 Comparative Example 1 135.0 Comparative Example 2 135.6 Comparative Example 3 132.0 Comparative Example 4 117.8 Comparative Example 5 119.7 Comparative Example 6 122.4 Comparative Example 7 115.3 Comparative Example 8 110.5 Comparative Example 9 127.9 Comparative Example 10 129.3 Comparison 103.3
[0072] Experimental Example 3
[0073] Effects of the single substance in fine powder form or the combination in fine powder form prepared in the treatment groups of Examples 1-3 and Comparative Examples 1-10 on the antioxidant level in Caenorhabditis elegans under oxidative stress
[0074] The fine powder compositions in Examples 1-3 and Comparative Examples 1-10 were added to the synchronized L4 stage N2 wild-type Caenorhabditis elegans at a final concentration of 10 μg / mL, respectively, and an equal volume of S Medium solution was added to the control group. After culturing at 20°C for 48 hours, paraquat was added at a final concentration of 70 mmol / L to treat the nematodes with oxidative stress. The nematodes were collected, homogenized in an ice bath, centrifuged, and the supernatant was finally taken. The ROS, SOD, CAT, and MDA contents were determined using a kit. The results are shown in Figure 3-6 The results show that the composition in fine powder form prepared in Examples 1-3 can significantly reduce the content of active oxygen in nematodes under oxidative stress conditions, increase the activity of superoxide dismutase and catalase, and reduce the content of malondialdehyde, indicating that it has good antioxidant activity.
[0075] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.
Claims
1. A sugarcane polyphenol composition with anti-aging effect, characterized in that: These include gallic acid, quercetin, catechins, rutin, and epigallocatechin-3-glucopyranoside.
2. The sugarcane polyphenol composition according to claim 1, characterized in that: Calculated by weight, it comprises 5-10 parts of gallic acid, 3-9 parts of quercetin, 3-6 parts of catechol, 2-5 parts of rutin and 2-5 parts of epigallocatechin gallate.
3. The sugarcane polyphenol composition according to claim 2, characterized in that: Calculated by weight, the composition comprises 8.9 parts of gallic acid, 5.6 parts of quercetin, 4.2 parts of catechol, 3.4 parts of rutin and 2.9 parts of epigallocatechin gallate.
4. A method for preparing the sugarcane polyphenol composition according to any one of claims 1 to 3, characterized in that: The following steps are involved: Gallic acid, quercetin, catechol, rutin and epigallocatechin-3-ol are fully mixed to obtain a mixture, and then the mixture is ultrafinely ground and passed through a sieve to obtain a sugarcane polyphenol composition in the form of fine powder.
5. The preparation method according to claim 4, characterized in that: The pore size of the sieve is 0.5 mm.
6. Use of the sugarcane polyphenol composition according to any one of claims 1 to 3 in the preparation of anti-aging products.
7. The use according to claim 6, characterized in that: The anti-aging approach includes extending lifespan and improving the ability to resist oxidative stress.
8. An anti-aging product, characterized in that: The effective ingredients include the sugarcane polyphenol composition according to any one of claims 1 to 3.