Kidney-tonifying and bone-tonifying oral liquid and preparation method thereof

Abstract

The invention relates to oral liquid for tonifying kidney and bone and a preparation method thereof, and belongs to the technical field of medicine, the oral liquid contains rhizoma drynariae, polygonum multiflorum, poria cocos, teasel root, radix paeoniae alba, angelica sinensis, codonopsis pilosula, prepared rehmannia root, polygonatum kingianum, fructus lycii, native copper and pericarpium citri reticulatae; the rhizoma drynariae is a product obtained by performing enzymolysis on a whole rhizoma drynariae plant through hemicellulase and pectinase, performing enzymolysis through bromelain and papain and performing enzymolysis through nattokinase; polygonum multiflorum is a product obtained by carrying out enzymolysis on Polygonum multiflorum tuberous roots through hemicellulase and pectinase, then carrying out enzymolysis through bromelain and papain and then carrying out enzymolysis through gelatinase. Polygonatum sibiricum is a product obtained by performing enzymolysis on rhizome of Polygonatum sibiricum through hemicellulase and pectinase, performing enzymolysis through bromelain and papain, and performing fermentation through bacillus licheniformis and saccharomyces boulardii. All the active components are matched for use, kidney function metabolism can be well enhanced, bone mineral density is increased, the treatment course is short, the effect taking speed is high, safety and reliability are achieved, and medication compliance is effectively improved.

Description

Technical Field

[0001] The invention belongs to the technical field of medicines, and in particular relates to a kidney-tonifying and bone-tonifying oral liquid and a preparation method thereof. Background Art

[0002] The human skeletal system is an important supporting structure of the body. It not only gives us the shape of our body, but also participates in a variety of physiological functions, such as movement, protection of internal organs, storage of minerals, etc. The kidneys also play a vital role in the human body. In addition to being responsible for excreting metabolic waste, regulating water and electrolyte balance and acid-base balance, they are also closely related to bone health.

[0003] From the perspective of traditional Chinese medicine theory, the kidney is responsible for bone and marrow production. If the kidney essence is sufficient, the bone marrow biochemistry is active, and the bones are nourished and strong. If the kidney essence is deficient and the bone marrow is malnourished, it will lead to skeletal dysplasia, fragile bones, sore waist and knees, muscle and bone pain, loose teeth and other symptoms. In modern medical research, it is also found that the kidney regulates the metabolism of minerals such as calcium and phosphorus through the endocrine system, affecting bone formation and absorption. The active vitamin D secreted by the kidney can promote the intestinal absorption of calcium, regulate blood calcium levels, and thus affect bone mineralization. In addition, the kidney is also involved in the metabolism and regulation of a variety of hormones, which play a key role in the activity of bone cells and the balance of bone metabolism.

[0004] With the aging of the population, the incidence of bone diseases is increasing, becoming an important issue that seriously affects people's quality of life and health. Osteoporosis is one of the most common bone diseases, especially in the middle-aged and elderly population. The patient's bone mass gradually decreases, and the microstructure of bone tissue is destroyed, resulting in increased bone brittleness and prone to fractures. According to statistics, the number of osteoporosis patients worldwide is huge, which has brought a heavy economic burden to society and families.

[0005] In addition to conventional food and health care products, drug therapy plays an important role in the treatment of kidney essence deficiency and bone marrow malnutrition. In traditional medicine, the concept of nourishing the kidney and strengthening the bones has a long history. Through long-term clinical practice and theoretical summary, Chinese medicine has accumulated rich experience and methods. Many Chinese medicines are believed to have the effect of nourishing the kidney and strengthening the bones, such as Rehmannia glutinosa, Cornus officinalis, Lycium barbarum, Epimedium, Psoralea corylifolia, etc. These Chinese medicines regulate the functions of the internal organs and the circulation of qi and blood in the human body through the effects of nourishing the kidney and replenishing essence, nourishing yin and strengthening yang, and promoting blood circulation and removing blood stasis, so as to achieve the purpose of nourishing bones and promoting bone repair and regeneration. However, the conditioning effect of Chinese medicine is usually slow, the course of treatment is long, and the compliance of long-term use is poor, which often cannot meet the modern people's demand for rapid drug effects. Therefore, it is necessary to continuously develop and improve drug preparation technology to improve the efficacy of nourishing the kidney and strengthening the bones and shorten the course of treatment. Summary of the invention

[0006] The existing kidney-tonifying and bone-tonifying drugs have the problems of unclear short-term efficacy, long course of treatment, and poor compliance in long-term use, which often cannot meet the modern people's demand for rapid drug effects. The present invention provides a kidney-tonifying and bone-tonifying oral liquid and a preparation method thereof, the oral liquid contains active ingredients such as drynail rhizome, polygonum multiflorum, tuckahoe, dipsacus, white peony root, angelica, codonopsis pilosula, rehmannia root, polygonatum, wolfberry, natural copper and tangerine peel; wherein drynail rhizome, polygonum multiflorum and polygonatum are extracts obtained by specific enzymatic hydrolysis or fermentation; each active ingredient is used in combination according to a certain proportion, which can well enhance kidney function metabolism and increase bone density, with a short course of treatment, rapid effect, safety and reliability, and effective improvement of medication compliance, meeting the modern people's demand for rapid drug effects. The specific technical scheme is as follows:

[0007] A kidney-tonifying and bone-tonifying oral liquid, comprising the following main raw materials in parts by weight: 5 to 8 parts of drynariae, 3 to 6 parts of polygonum multiflorum, 3 to 6 parts of tuckahoe, 1 to 3 parts of radix dipsaci, 0.5 to 2 parts of white peony root, 0.5 to 1 part of angelica sinensis, 0.1 to 0.3 parts of codonopsis pilosula, 0.1 to 0.3 parts of rehmannia root, 0.1 to 0.3 parts of polygonatum, 0.1 to 0.3 parts of wolfberry, 0.05 to 0.1 parts of natural copper, and 0.05 to 0.1 parts of tangerine peel; the drynariae is a drynariae extract, which is obtained by co-production of the whole drynariae with hemicellulase and pectinase The extract is obtained by enzymolysis with bromelain and papain, and finally by nattokinase; the Polygonum multiflorum is a Polygonum multiflorum extract, which is an extract obtained by enzymolysis of Polygonum multiflorum tuber with hemicellulase and pectinase, then with bromelain and papain, and finally by chymotrypsin; the Polygonatum sibiricum is a Polygonatum sibiricum extract, which is an extract obtained by enzymolysis of Polygonatum sibiricum rhizome with hemicellulase and pectinase, then with bromelain and papain, and then fermented with Bacillus licheniformis and Saccharomyces boulardii.

[0008] In the above-mentioned oral liquid, the oral liquid also includes auxiliary materials in the following mass percentages: 5% to 20% of the total mass of the main raw material sucrose, 0.03% to 0.06% of the total mass of the main raw material benzoic acid, 0.05% to 0.1% of the total mass of the main raw material ethylparaben, and 10 to 30 times the total mass of the main raw material liquor; the alcohol content of the liquor is 3° to 12°.

[0009] In the above-mentioned oral liquid, the preparation method of the drynaria extract comprises the following steps: taking the whole plant of drynaria, crushing it, adding water, adjusting the pH to 5.0-5.5, adding hemicellulase and pectinase, enzymolysis at 45°C-55°C for 3h-4h, adjusting the pH to 6.0-7.5, adding bromelain and papain, enzymolysis at 50°C-60°C for 1h-1.5h, heating up to inactivate the enzyme, cooling down, adjusting the pH to 7.5-8.5, adding nattokinase, enzymolysis at 40°C-45°C for 1.5h-2h, heating up to inactivate the enzyme, cooling down, filtering with a sieve, taking the filtrate, and freeze-drying to obtain the drynaria extract.

[0010] In the preparation method of the above-mentioned drynaria extract, the amount of water added is 8 to 12 times the mass of the whole plant of drynaria; the amount of hemicellulase, pectinase, bromelain and papain added is 0.5% to 2% of the mass of the whole plant of drynaria; the amount of nattokinase added is 1.0% to 2.5% of the mass of the whole plant of drynaria; the temperature for heating and inactivating the enzyme is 80°C to 90°C, and the time for heating and inactivating the enzyme is 10 min to 20 min; the mesh size of the sieve is 250 mesh to 325 mesh.

[0011] In the above-mentioned oral liquid, the preparation method of the Polygonum multiflorum extract comprises the following steps: taking Polygonum multiflorum root tubers, crushing them, adding water, adjusting the pH to 5.0-5.5, adding hemicellulase and pectinase, enzymolysis at 45°C-55°C for 3h-4h, adjusting the pH to 6.0-7.5, adding bromelain and papain, enzymolysis at 50°C-60°C for 1h-1.5h, heating to inactivate the enzymes, cooling down, adjusting the pH to 7.0-8.0, adding protease, enzymolysis at 36°C-38°C for 1h-1.5h, heating to inactivate the enzymes, cooling down, filtering with a sieve, taking the filtrate, and freeze-drying to obtain the Polygonum multiflorum extract.

[0012] In the preparation method of the above-mentioned Polygonum multiflorum extract, the amount of water added is 8 to 12 times the mass of the Polygonum multiflorum root tuber; the amount of hemicellulase, pectinase, bromelain and papain added is 0.5% to 2% of the mass of the Polygonum multiflorum root tuber; the amount of chondrase added is 0.3% to 1.0% of the mass of the Polygonum multiflorum root tuber; the temperature for heating and inactivating the enzyme is 80°C to 90°C, and the time for heating and inactivating the enzyme is 10 min to 20 min; the mesh size of the sieve is 250 mesh to 325 mesh.

[0013] In the above oral liquid, the preparation method of the polygonatum extract comprises the following steps: taking the rhizome of polygonatum, crushing it, adding water, adjusting the pH to 5.0-5.5, adding hemicellulase and pectinase, enzymolysis at 45°C-55°C for 3h-4h, adjusting the pH to 6.0-7.5, adding bromelain and papain, enzymolysis at 50°C-60°C for 1h-1.5h, heating to inactivate the enzymes, cooling to room temperature, and obtaining an enzymolysis solution; adding glucose, disodium hydrogen phosphate, and ammonium chloride to the enzymolysis solution, adjusting the pH to 6.5-7.5, adding Bacillus licheniformis, aerobically fermenting at 30°C-37°C for 12h-15h, then adjusting the pH to 4.5-5.0, adding Saccharomyces boulardii, anaerobically fermenting at 36°C-38°C for 12h-15h, sterilizing at high temperature, cooling, filtering with a sieve, taking the filtrate, and freeze-drying to obtain the polygonatum extract.

[0014] In the preparation method of the above-mentioned polygonatum extract, the amount of water added is 10 to 15 times the mass of the polygonatum rhizome; the amount of hemicellulase, pectinase, bromelain and papain added is 0.5% to 2% of the mass of the polygonatum rhizome; the amount of glucose added is 3% to 5% of the mass of the enzymatic solution; the amount of disodium hydrogen phosphate added is 0.1% to 0.5% of the mass of the enzymatic solution; the amount of ammonium chloride added is 1.5% to 2.5% of the mass of the enzymatic solution; the amount of Bacillus licheniformis and Saccharomyces boulardii added is 0.5% to 2% of the mass of the polygonatum rhizome; the temperature of heating and inactivating the enzyme is 80°C to 90°C, and the time of heating and inactivating the enzyme is 10min to 20min; the temperature of high-temperature sterilization is 121°C to 125°C, and the time of high-temperature sterilization is 15min to 25min; the mesh number of the sieve is 250 mesh to 325 mesh.

[0015] In the above oral liquid, the Poria cocos, Dipsacus asper, White Peony Root, Angelica sinensis, Codonopsis pilosula, Rehmannia root, Lycium barbarum and Tangerine peel are all extracts obtained by percolating traditional Chinese medicine dry powder through ethanol.

[0016] The preparation method of the above-mentioned Yishen Bugu oral liquid comprises the following steps:

[0017] According to the weight proportions, drynaria root, polygonum multiflorum, poria, dipsaci, white peony root, angelica, codonopsis, rehmannia root, polygonatum sibiricum, wolfberry, natural copper and tangerine peel are uniformly mixed to obtain a mixture; the mixture is mixed with preservatives, flavoring agents and solvents which are allowed to be added to the oral liquid of the medicine to prepare an oral liquid for nourishing the kidney and bones.

[0018] Compared with the prior art, the kidney-tonifying and bone-nourishing oral liquid and its preparation method provided by the present invention have the following beneficial effects:

[0019] 1. The oral liquid of the present invention contains ingredients such as drynaria, polygonum multiflorum, tuckahoe, dipsaci, white peony root, angelica, codonopsis, rehmannia root, polygonatum, wolfberry, natural copper and tangerine peel; the active ingredients are used in a certain proportion, which can well enhance kidney function metabolism and increase bone density, with a short course of treatment, fast effect, safety and reliability, and effectively improve medication compliance, thus meeting the demand of modern people for rapid drug effect.

[0020] Second, the present invention's drynaria extract is drynaria extract, which is drynaria whole plant by hemicellulase and pectinase co-enzyme hydrolysis, then by bromelain and papain co-enzyme hydrolysis, and finally by nattokinase to obtain the extract. Hemicellulase and pectinase enzymolysis can destroy the plant cell wall structure, release more active ingredients, help bromelain, papain, nattokinase further hydrolyze protein and other macromolecular substances, produce small molecule peptides, amino acids and other active ingredients, play a high-efficiency role, for kidney and bone tonifying provide a better physiological environment. The ingredients in the extract can stimulate the proliferation and differentiation of osteoblasts, promote the synthesis and mineralization of bone matrix, and increase bone density. Regulate the expression of cytokines and growth factors related to bone metabolism, such as bone morphogenetic protein (BMP), insulin-like growth factor (IGF), etc., promote bone formation. The enzymolysis product of nattokinase can improve blood circulation, provide sufficient nutrients and oxygen for kidney and bone, and promote cell metabolism and repair. The extract after enzymatic hydrolysis contains a variety of nutrients, such as amino acids, minerals, etc., which provide necessary nutritional support for kidney and bone cells and promote their normal function and repair.

[0021] 3. The present invention is a Polygonum multiflorum extract, which is a Polygonum multiflorum root tuber that is hydrolyzed by hemicellulase and pectinase, then hydrolyzed by bromelain and papain, and finally hydrolyzed by clotting protease to obtain an extract. Hemicellulase and pectinase hydrolysis can destroy the plant cell wall structure, release more active ingredients, help bromelain and papain to further hydrolyze macromolecular substances such as protein, produce active ingredients such as small molecule peptides and amino acids, and bromelain and papain cooperate with clotting protease to hydrolyze, which can produce specific hydrolysis products, which play a unique role in tonifying the kidney and strengthening the bones. The ingredients in the extract can stimulate the proliferation and differentiation of osteoblasts, promote the synthesis and mineralization of bone matrix. Regulate the expression of osteogenic related genes such as bone morphogenetic protein (BMP), Runt-related transcription factor 2 (Runx2). Polygonum multiflorum extract can regulate the secretion of sex hormones, growth hormone, etc., and these hormones have important regulatory effects on bone metabolism and kidney function. It has antioxidant activity, removes free radicals in the body, and reduces the damage of oxidative stress to kidney and bone cells. It can improve the blood circulation of kidneys and bones, providing them with sufficient nutrients and oxygen. Good microcirculation helps cell metabolism and repair, and promotes the health of kidneys and bones. The extract after enzymatic hydrolysis contains a variety of nutrients, such as amino acids, trace elements, etc., which provide necessary nutritional support for kidney and bone cells and promote their normal function and repair.

[0022] Fourth, the present invention is a polygonatum extract, which is an extract obtained by co-enzyme hydrolysis of polygonatum rhizome by hemicellulase and pectinase, then co-enzyme hydrolysis by bromelain and papain, and then fermentation by Bacillus licheniformis and Saccharomyces boulardii. Hemicellulase and pectinase enzymolysis can destroy the plant cell wall structure, release more active ingredients, help bromelain and papain to further hydrolyze macromolecules such as protein, produce active ingredients such as small molecule peptides and amino acids, which are beneficial to the body's absorption and utilization. Bacillus licheniformis and Saccharomyces boulardii fermentation can produce new active metabolites and enhance the efficacy of the extract. The fermentation process can change the structure and activity of the original components of polygonatum and produce substances with specific biological activity. The components in the extract can stimulate the proliferation and differentiation of osteoblasts and promote the synthesis and mineralization of bone matrix. It can maintain bone metabolism balance and prevent bone loss. It can regulate the secretion of sex hormones, growth hormones, etc., which have important regulatory effects on bone metabolism and kidney function. The polygonatum extract has antioxidant activity, removes free radicals in the body, and reduces the damage of oxidative stress to kidney and bone cells. Fermented microorganisms such as Saccharomyces boulardii can regulate the balance of intestinal flora and improve intestinal function. A good intestinal flora helps the absorption and metabolism of nutrients and has a positive impact on kidney and bone health.

[0023] 5. The ethanol percolation extract of Poria cocos can regulate water and salt metabolism in the body and help maintain normal physiological functions of the kidneys. The kidneys play a key role in water and salt metabolism, and a good water and salt balance is essential for kidney health. It can participate in the metabolic regulation of minerals such as calcium and phosphorus. Proper calcium and phosphorus metabolism is very important for the healthy development of bones and the maintenance of bone density. It can affect the kidney-related endocrine system and regulate hormone levels. It can also have a regulatory effect on the bone-related endocrine system, such as affecting the metabolism of vitamin D and parathyroid hormone, and indirectly promoting bone health.

[0024] 6. The ethanol percolation extract of Dipsacus asper contains certain ingredients that can stimulate the proliferation, differentiation and functional activity of osteoblasts. Osteoblasts are responsible for the synthesis and mineralization of bone matrix and promote bone formation. They can regulate some hormones or active substances secreted by the kidneys and indirectly affect bone metabolism. For example, they affect the renin-angiotensin system and vitamin D activation, which can maintain normal kidney function while having a positive effect on bone health.

[0025] 7. The ethanol percolation extracts of white peony root, Chinese angelica root, codonopsis root and prepared rehmannia root have a regulatory effect on the hormones and active substances secreted by the kidneys. For example, it affects the renin-angiotensin-aldosterone system, maintains the normal physiological function of the kidneys, and indirectly has a positive effect on bone health. It can have a certain regulatory effect on the metabolism and balance of sex hormones. Sex hormones play an important role in maintaining bone density and bone metabolism. Appropriate sex hormone levels help to nourish the kidneys and strengthen bones. It can regulate the immune system and enhance the body's immunity. It can have the effect of dilating blood vessels, promoting blood circulation in the kidneys, providing the kidneys with sufficient oxygen and nutrients, and promoting the metabolism and repair of kidney cells.

[0026] 8. The ethanol percolation extract of wolfberry contains rich antioxidant ingredients, such as wolfberry polysaccharides and carotenoids. These ingredients can remove free radicals in the body and reduce the damage of oxidative stress to kidney and bone cells. Free radicals are one of the important factors leading to cell aging and damage. Through antioxidant effects, wolfberry extract can protect the structure and function of kidney and bone cells.

[0027] 9. Natural copper can activate osteoblasts through certain mechanisms, promoting their proliferation, differentiation and functional activity. Osteoblasts are responsible for the synthesis and mineralization of bone matrix, and play a key role in fracture healing and bone repair. It can affect the expression of bone metabolism-related factors such as bone morphogenetic protein (BMP) and insulin-like growth factor (IGF), which have an important regulatory effect on bone formation and repair. Natural copper can help improve blood circulation at the fracture site, provide sufficient nutrients and oxygen to bone tissue, and promote the formation of callus and fracture healing.

[0028] 10. The ethanol percolation extract of tangerine peel can promote gastrointestinal motility, improve digestive function, and help absorb nutrients. Good nutrient absorption is essential for maintaining normal metabolism and health of the kidneys and bones. It can regulate the intestinal flora and maintain the balance of intestinal microecology. Healthy intestinal flora helps the synthesis and absorption of nutrients, and indirectly has a positive impact on kidney and bone health. Tangerine peel contains antioxidant ingredients such as flavonoids. The extract can remove free radicals in the body and reduce the damage of oxidative stress to kidney and bone cells. Tangerine peel extract can inhibit the production and release of inflammatory factors and reduce the inflammatory response of the kidneys and bones. Inflammation can damage the kidneys and bones, and inhibiting inflammation can help maintain their normal function. DETAILED DESCRIPTION

[0029] The present invention is further described below in conjunction with specific implementation cases, but the present invention is not limited to these embodiments.

[0030] Example 1

[0031] A kidney-tonifying and bone-tonifying oral liquid, comprising the following main raw materials in mass fractions: 6.5 parts of Drynaria fortunei, 4.5 parts of Polygonum multiflorum, 4.5 parts of Poria cocos, 2 parts of Dipsacus asper, 1.2 parts of White Peony Root, 1 part of Angelica sinensis, 0.2 parts of Codonopsis pilosula, 0.2 parts of Rehmannia glutinosa, 0.2 parts of Polygonatum sibiricum, 0.2 parts of Lycium barbarum, 0.08 parts of natural copper, and 0.08 parts of dried orange peel; the oral liquid also comprises the following auxiliary materials in mass percentages: 10% of the total mass of the main raw materials in sucrose, 0.05% of the total mass of the main raw materials in benzoic acid, 0.08% of the total mass of the main raw materials in ethylparaben, and 20 times the total mass of the main raw materials in liquor; the alcohol content of the liquor is 7°. Among them, Poria cocos, Dipsacus asper, White Peony Root, Angelica sinensis, Codonopsis pilosula, Rehmannia glutinosa, Lycium barbarum, and dried orange peel are extracts obtained by ethanol percolation of Chinese medicine dry powder (taking the ethanol percolation liquid and freeze-drying to obtain the extract). Native copper is obtained from pyrite after removal of impurities, calcination, vinegar quenching and crushing.

[0032] Among them, the drynaria is a drynaria extract, and the preparation method of the drynaria extract comprises the following steps: taking a whole plant of drynaria, crushing it, adding water that is 10 times the mass of the whole plant of drynaria, adjusting the pH to 5.2, adding hemicellulase and pectinase that are 1% by mass of the whole plant of drynaria, enzymolysis at 50°C for 3.5 hours, adjusting the pH to 7.0, adding bromelain and papain that are 1% by mass of the whole plant of drynaria, enzymolysis at 55°C for 1 hour, heating to 85°C to inactivate the enzyme for 15 minutes, cooling down, adjusting the pH to 8.0, adding nattokinase that is 2% by mass of the whole plant of drynaria, enzymolysis at 42°C for 1.5 hours, heating to 85°C to inactivate the enzyme for 15 minutes, cooling down, filtering with a 325-mesh sieve, taking the filtrate, and freeze-drying to obtain the drynaria extract.

[0033] The Polygonum multiflorum is a Polygonum multiflorum extract, and the preparation method of the Polygonum multiflorum extract comprises the following steps: taking Polygonum multiflorum tubers, crushing them, adding water 10 times the mass of Polygonum multiflorum tubers, adjusting the pH to 5.2, adding hemicellulase and pectinase each of which is 1% by mass of Polygonum multiflorum tubers, enzymolysis at 50 DEG C for 3.5 hours, adjusting the pH to 6.8, adding bromelain and papain each of which is 1% by mass of Polygonum multiflorum tubers, enzymolysis at 55 DEG C for 1 hour, heating to 85 DEG C to inactivate the enzyme for 15 minutes, cooling down, adjusting the pH to 7.5, adding 0.5% of the mass of Polygonum multiflorum tubers, enzymolysis at 37 DEG C for 1 hour, heating to 85 DEG C to inactivate the enzyme for 15 minutes, cooling down, filtering with a 325-mesh screen, taking the filtrate, and freeze-drying to obtain the Polygonum multiflorum extract.

[0034] The invention discloses a method for preparing the extract of Polygonatum sibiricum, comprising the steps of: taking the rhizome of Polygonatum sibiricum, crushing it, adding water of 12 times the weight of the rhizome of Polygonatum sibiricum, adjusting the pH to 5.2, adding hemicellulase and pectinase of 1% by weight of the rhizome of Polygonatum sibiricum, performing enzymolysis at 50°C for 3.5 hours, adjusting the pH to 6.8, adding bromelain and papain of 1.2% by weight of the rhizome of Polygonatum sibiricum, performing enzymolysis at 55°C for 1 hour, heating to 85°C to inactivate the enzymes for 15 minutes, cooling to room temperature, and obtaining an enzymolysis solution; adding glucose of 4% by weight of the enzymolysis solution, sodium dihydrogen phosphate of 0.3% by weight of the enzymolysis solution, and ammonium chloride of 2% by weight of the enzymolysis solution, adjusting the pH to 7.0, adding Bacillus licheniformis of 1.2% by weight of the rhizome of Polygonatum sibiricum, performing aerobic fermentation at 35°C for 14 hours, then adjusting the pH to 4.8, adding Saccharomyces boulardii of 1.2% by weight of the rhizome of Polygonatum sibiricum, performing anaerobically fermentation at 37°C for 14 hours, and performing high temperature sterilization at 121°C for 20 minutes. n, after cooling, filter with a 325-mesh sieve, take the filtrate, and freeze-dry to obtain the polygonatum sibiricum extract.

[0035] The preparation method of the above-mentioned Yishen Bugu oral liquid comprises the following steps:

[0036] According to the weight proportions, drynaria extract, polygonum multiflorum extract, poria extract, dipsaci extract, white peony extract, angelica extract, codonopsis extract, rehmannia root extract, polygonatum sibiricum extract, wolfberry fruit extract, natural copper and tangerine peel extract are uniformly mixed to obtain a mixture; the mixture is mixed with sucrose, benzoic acid, ethylparaben and liquor to prepare an oral liquid for nourishing the kidney and bones.

[0037] Example 2

[0038] A kidney-tonifying and bone-tonifying oral liquid, the oral liquid comprises the following main raw materials in parts by weight: 5 parts of Drynaria fortunei, 3 parts of Polygonum multiflorum, 3 parts of Poria cocos, 1 part of Dipsacus asper, 0.5 parts of White Peony Root, 0.5 parts of Angelica sinensis, 0.1 parts of Codonopsis pilosula, 0.1 parts of Rehmannia glutinosa, 0.1 parts of Polygonatum sibiricum, 0.1 parts of Lycium barbarum, 0.05 parts of natural copper, 0.05 parts of dried orange peel; the oral liquid also comprises the following auxiliary materials in parts by weight: 5% of the total weight of the main raw materials in sucrose, 0.03% of the total weight of the main raw materials in benzoic acid, 0.05% of the total weight of the main raw materials in ethylparaben, and 10 times the total weight of the main raw materials in liquor; the alcohol content of the liquor is 3°. Among them, Poria cocos, Dipsacus asper, White Peony Root, Angelica sinensis, Codonopsis pilosula, Rehmannia glutinosa, Lycium barbarum and dried orange peel are all extracts obtained by ethanol percolation of Chinese medicine dry powder (taking ethanol percolation liquid and freeze-drying to obtain extract). Natural copper is natural copper obtained by removing impurities, calcining, vinegar quenching and crushing pyrite.

[0039] Among them, the drynaria is a drynaria extract, and the preparation method of the drynaria extract comprises the following steps: taking a whole plant of drynaria, crushing it, adding water of 8 times the mass of the whole plant of drynaria, adjusting the pH to 5.0, adding hemicellulase and pectinase of 0.5% by mass of the whole plant of drynaria, enzymolysis at 45°C for 3 hours, adjusting the pH to 6.0, adding bromelain and papain of 0.5% by mass of the whole plant of drynaria, enzymolysis at 50°C for 1 hour, heating to 80°C to inactivate the enzyme for 10 minutes, cooling down, adjusting the pH to 7.5, adding nattokinase of 1.0% by mass of the whole plant of drynaria, enzymolysis at 40°C for 1.5 hours, heating to 80°C to inactivate the enzyme for 10 minutes, cooling down, filtering with a 250-mesh sieve, taking the filtrate, and freeze-drying to obtain the drynaria extract.

[0040] The Polygonum multiflorum is a Polygonum multiflorum extract, and the preparation method of the Polygonum multiflorum extract comprises the following steps: taking Polygonum multiflorum tubers, crushing them, adding water of 8 times the mass of Polygonum multiflorum tubers, adjusting the pH to 5.0, adding hemicellulase and pectinase of 0.5% by mass of Polygonum multiflorum tubers, enzymolysis at 45 DEG C for 3 hours, adjusting the pH to 6.0, adding bromelain and papain of 0.5% by mass of Polygonum multiflorum tubers, enzymolysis at 50 DEG C for 1 hour, heating to 80 DEG C to inactivate the enzyme for 10 minutes, cooling down, adjusting the pH to 7.0, adding 0.3% of the mass of Polygonum multiflorum tubers, enzymolysis at 36 DEG C for 1 hour, heating to 80 DEG C to inactivate the enzyme for 10 minutes, cooling down, filtering with a 250-mesh screen, taking the filtrate, and freeze-drying to obtain the Polygonum multiflorum extract.

[0041] The invention discloses a method for preparing the extract of polygonatum sibiricum, comprising the steps of: taking rhizomes of polygonatum sibiricum, crushing them, adding water 10 times the weight of the rhizomes of polygonatum sibiricum, adjusting the pH to 5.0, adding hemicellulase and pectinase 0.5% by weight of the rhizomes of polygonatum sibiricum, performing enzymolysis at 45°C for 3h, adjusting the pH to 6.0, adding bromelain and papain 0.5% by weight of the rhizomes of polygonatum sibiricum, performing enzymolysis at 50°C for 1h, heating to 80°C to inactivate the enzymes for 10min, and cooling to room temperature to obtain an enzymolysis solution; adding bromoproteinase to the enzymolysis solution; 3% of glucose by weight of the enzymatic hydrolyzate, 0.1% of disodium hydrogen phosphate by weight of the enzymatic hydrolyzate, and 1.5% of ammonium chloride by weight of the enzymatic hydrolyzate were added to adjust the pH value to 6.5, 0.5% of Bacillus licheniformis by weight of the rhizome of Polygonatum sibiricum was added, aerobic fermentation was carried out at 30°C for 12 hours, then the pH value was adjusted to 4.5, 0.5% of Saccharomyces boulardii by weight of the rhizome of Polygonatum sibiricum was added, anaerobically fermented at 36°C for 12 hours, high-temperature sterilization was carried out at 121°C for 15 minutes, after cooling, filtration was carried out using a 250-mesh sieve, the filtrate was taken, and freeze-dried to obtain a Polygonatum sibiricum extract.

[0042] The preparation method of the above-mentioned Yishen Bugu oral liquid comprises the following steps:

[0043] According to the weight proportions, drynaria extract, polygonum multiflorum extract, poria extract, dipsaci extract, white peony extract, angelica extract, codonopsis extract, rehmannia root extract, polygonatum sibiricum extract, wolfberry fruit extract, natural copper and tangerine peel extract are uniformly mixed to obtain a mixture; the mixture is mixed with sucrose, benzoic acid, ethylparaben and liquor to prepare an oral liquid for nourishing the kidney and bones.

[0044] Example 3

[0045] A kidney-tonifying and bone-tonifying oral liquid, the oral liquid comprises the following main raw materials in parts by weight: 8 parts of Drynaria fortunei, 6 parts of Polygonum multiflorum, 6 parts of Poria cocos, 3 parts of Dipsacus asper, 2 parts of White Peony Root, 1 part of Angelica sinensis, 0.3 parts of Codonopsis pilosula, 0.3 parts of Rehmannia glutinosa, 0.3 parts of Polygonatum sibiricum, 0.3 parts of Lycium barbarum, 0.1 parts of natural copper, and 0.1 parts of dried orange peel; the oral liquid also comprises the following auxiliary materials in parts by weight: 20% of the total weight of the main raw materials in sucrose, 0.06% of the total weight of the main raw materials in benzoic acid, 0.1% of the total weight of the main raw materials in ethylparaben, and 30 times the total weight of the main raw materials in liquor; the alcohol content of the liquor is 12°. Among them, Poria cocos, Dipsacus asper, White Peony Root, Angelica sinensis, Codonopsis pilosula, Rehmannia glutinosa, Lycium barbarum, and dried orange peel are all extracts obtained by ethanol percolation of Chinese medicine dry powder (taking ethanol percolation liquid and freeze-drying to obtain extract). Natural copper is natural copper obtained by removing impurities, calcining, vinegar quenching, and crushing pyrite.

[0046] Among them, the drynaria is a drynaria extract, and the preparation method of the drynaria extract comprises the following steps: taking a whole plant of drynaria, crushing it, adding water 12 times the mass of the whole plant of drynaria, adjusting the pH to 5.5, adding hemicellulase and pectinase each of which is 2% by mass of the whole plant of drynaria, enzymolysis at 55°C for 4 hours, adjusting the pH to 7.5, adding bromelain and papain each of which is 2% by mass of the whole plant of drynaria, enzymolysis at 60°C for 1.5 hours, heating to 90°C to inactivate the enzyme for 20 minutes, cooling down, adjusting the pH to 8.5, adding nattokinase each of which is 2.5% by mass of the whole plant of drynaria, enzymolysis at 45°C for 2 hours, heating to 90°C to inactivate the enzyme for 20 minutes, cooling down, filtering with a 325-mesh sieve, taking the filtrate, and freeze-drying to obtain the drynaria extract.

[0047] The Polygonum multiflorum is a Polygonum multiflorum extract, and the preparation method of the Polygonum multiflorum extract comprises the following steps: taking Polygonum multiflorum tubers, crushing them, adding water 12 times the mass of Polygonum multiflorum tubers, adjusting the pH to 5.5, adding hemicellulase and pectinase each of which is 2% by mass of Polygonum multiflorum tubers, enzymolysis at 55 DEG C for 4 hours, adjusting the pH to 7.5, adding bromelain and papain each of which is 2% by mass of Polygonum multiflorum tubers, enzymolysis at 60 DEG C for 1.5 hours, heating to 90 DEG C to inactivate the enzyme for 20 minutes, cooling down, adjusting the pH to 8.0, adding 1.0% of the mass of Polygonum multiflorum tubers, enzymolysis at 38 DEG C for 1.5 hours, heating to 90 DEG C to inactivate the enzyme for 20 minutes, cooling down, filtering with a 325-mesh screen, taking the filtrate, and freeze-drying to obtain the Polygonum multiflorum extract.

[0048] The polygonatum is a polygonatum extract, and the preparation method of the polygonatum extract comprises the following steps: taking the rhizome of polygonatum, crushing it, adding water 15 times the weight of the rhizome of polygonatum, adjusting the pH to 5.5, adding hemicellulase and pectinase each at 2% by weight of the rhizome of polygonatum, enzymolysis at 55°C for 4h, adjusting the pH to 7.5, adding bromelain and papain each at 2% by weight of the rhizome of polygonatum, enzymolysis at 60°C for 1.5h, heating to 90°C to inactivate the enzyme for 20min, cooling to room temperature to obtain an enzymolysis solution; adding the enzymolysis solution to the 5% glucose by weight of the enzymatic hydrolyzate, 0.5% disodium hydrogen phosphate by weight of the enzymatic hydrolyzate, and 2.5% ammonium chloride by weight of the enzymatic hydrolyzate were added to adjust the pH value to 7.5, 2% Bacillus licheniformis by weight of the rhizome of Polygonatum sibiricum was added, aerobically fermented at 37°C for 15 hours, then the pH value was adjusted to 5.0, 2% boulardii yeast by weight of the rhizome of Polygonatum sibiricum was added, anaerobically fermented at 38°C for 15 hours, high-temperature sterilized at 125°C for 25 minutes, filtered through a 325-mesh sieve after cooling, the filtrate was taken, and freeze-dried to obtain a Polygonatum sibiricum extract.

[0049] The preparation method of the above-mentioned Yishen Bugu oral liquid comprises the following steps:

[0050] According to the weight proportions, drynaria extract, polygonum multiflorum extract, poria extract, dipsaci extract, white peony extract, angelica extract, codonopsis extract, rehmannia root extract, polygonatum sibiricum extract, wolfberry fruit extract, natural copper and tangerine peel extract are uniformly mixed to obtain a mixture; the mixture is mixed with sucrose, benzoic acid, ethylparaben and liquor to prepare an oral liquid for nourishing the kidney and bones.

[0051] Example 4

[0052] A kidney-tonifying and bone-tonifying oral liquid, the oral liquid comprises the following main raw materials in parts by weight: 5 parts of Drynaria fortunei, 6 parts of Polygonum multiflorum, 3 parts of Poria cocos, 3 parts of Dipsacus asper, 0.5 parts of White Peony Root, 1 part of Angelica sinensis, 0.1 parts of Codonopsis pilosula, 0.3 parts of Rehmannia glutinosa, 0.1 parts of Polygonatum sibiricum, 0.3 parts of Lycium barbarum, 0.05 parts of natural copper, and 0.1 parts of dried orange peel; the oral liquid also comprises the following auxiliary materials in parts by weight: 5% of the total weight of the main raw materials in sucrose, 0.06% of the total weight of the main raw materials in benzoic acid, 0.05% of the total weight of the main raw materials in ethylparaben, and 30 times the total weight of the main raw materials in liquor; the alcohol content of the liquor is 3°. Among them, Poria cocos, Dipsacus asper, White Peony Root, Angelica sinensis, Codonopsis pilosula, Rehmannia glutinosa, Lycium barbarum, and dried orange peel are all extracts obtained by ethanol percolation of Chinese medicine dry powder (taking ethanol percolation liquid and freeze-drying to obtain extract). Natural copper is natural copper obtained by removing impurities, calcining, vinegar quenching, and crushing pyrite.

[0053] Among them, the drynaria is a drynaria extract, and the preparation method of the drynaria extract comprises the following steps: taking a whole plant of drynaria, crushing it, adding water 12 times the mass of the whole plant of drynaria, adjusting the pH to 5.0, adding hemicellulase and pectinase each of which is 2% by mass of the whole plant of drynaria, enzymolysis at 45°C for 4 hours, adjusting the pH to 6.0, adding bromelain and papain each of which is 2% by mass of the whole plant of drynaria, enzymolysis at 50°C for 1.5 hours, heating to 80°C to inactivate the enzyme for 20 minutes, cooling down, adjusting the pH to 7.5, adding nattokinase each of which is 2.5% by mass of the whole plant of drynaria, enzymolysis at 40°C for 2 hours, heating to 80°C to inactivate the enzyme for 20 minutes, cooling down, filtering with a 250-mesh sieve, taking the filtrate, and freeze-drying to obtain the drynaria extract.

[0054] The Polygonum multiflorum is a Polygonum multiflorum extract, and the preparation method of the Polygonum multiflorum extract comprises the following steps: taking Polygonum multiflorum tubers, crushing them, adding water of 12 times the mass of Polygonum multiflorum tubers, adjusting the pH to 5.0, adding hemicellulase and pectinase of 2% by mass of Polygonum multiflorum tubers, enzymolysis at 45 DEG C for 4 hours, adjusting the pH to 6.0, adding bromelain and papain of 2% by mass of Polygonum multiflorum tubers, enzymolysis at 50 DEG C for 1.5 hours, heating to 80 DEG C to inactivate the enzyme for 20 minutes, cooling down, adjusting the pH to 7.0, adding 1.0% of the mass of Polygonum multiflorum tubers, enzymolysis at 36 DEG C for 1.5 hours, heating to 80 DEG C to inactivate the enzyme for 20 minutes, cooling down, filtering with a 250-mesh screen, taking the filtrate, and freeze-drying to obtain the Polygonum multiflorum extract.

[0055] The invention discloses a method for preparing the extract of Polygonatum sibiricum, comprising the steps of: taking the rhizome of Polygonatum sibiricum, crushing it, adding 15 times the weight of water of the rhizome of Polygonatum sibiricum, adjusting the pH to 5.0, adding 2% of hemicellulase and pectinase of the rhizome of Polygonatum sibiricum by weight, performing enzymolysis at 45°C for 4 hours, adjusting the pH to 6.0, adding 2% of bromelain and papain of the rhizome of Polygonatum sibiricum by weight, performing enzymolysis at 50°C for 1.5 hours, heating to 80°C to inactivate the enzymes for 20 minutes, cooling to room temperature, and obtaining an enzymolysis solution; adding 3% of glucose of the enzymolysis solution by weight, 0.5% of disodium hydrogen phosphate of the enzymolysis solution by weight, and 1.5% of ammonium chloride of the enzymolysis solution by weight, adjusting the pH to 7.5, adding 0.5% of Bacillus licheniformis of the rhizome of Polygonatum sibiricum by weight, performing aerobic fermentation at 37°C for 12 hours, then adjusting the pH to 5.0, adding 0.5% of Saccharomyces boulardii of the rhizome of Polygonatum sibiricum by weight, performing anaerobically fermentation at 38°C for 12 hours, and performing high temperature sterilization at 125°C for 15 minutes. n, after cooling, filter with a 325-mesh sieve, take the filtrate, and freeze-dry to obtain the polygonatum sibiricum extract.

[0056] The preparation method of the above-mentioned Yishen Bugu oral liquid comprises the following steps:

[0057] According to the weight proportions, drynaria extract, polygonum multiflorum extract, poria extract, dipsaci extract, white peony extract, angelica extract, codonopsis extract, rehmannia root extract, polygonatum sibiricum extract, wolfberry fruit extract, natural copper and tangerine peel extract are uniformly mixed to obtain a mixture; the mixture is mixed with sucrose, benzoic acid, ethylparaben and liquor to prepare an oral liquid for nourishing the kidney and bones.

[0058] Example 5

[0059] A kidney-tonifying and bone-tonifying oral liquid, the oral liquid comprises the following main raw materials in parts by weight: 8 parts of Drynaria fortunei, 3 parts of Polygonum multiflorum, 6 parts of Poria cocos, 1 part of Dipsacus asper, 2 parts of White Peony Root, 0.5 parts of Angelica sinensis, 0.3 parts of Codonopsis pilosula, 0.1 parts of Rehmannia glutinosa, 0.3 parts of Polygonatum sibiricum, 0.1 parts of Lycium barbarum, 0.1 parts of natural copper, 0.05 parts of dried orange peel; the oral liquid also comprises the following auxiliary materials in parts by weight: 20% of the total weight of the main raw materials in sucrose, 0.03% of the total weight of the main raw materials in benzoic acid, 0.1% of the total weight of the main raw materials in ethylparaben, and 10 times the total weight of the main raw materials in liquor; the alcohol content of the liquor is 12°. Among them, Poria cocos, Dipsacus asper, White Peony Root, Angelica sinensis, Codonopsis pilosula, Rehmannia glutinosa, Lycium barbarum and dried orange peel are all extracts obtained by ethanol percolation of Chinese medicine dry powder (taking ethanol percolation liquid and freeze-drying to obtain extract). Natural copper is natural copper obtained by removing impurities, calcining, vinegar quenching and crushing pyrite.

[0060] Among them, the drynaria is a drynaria extract, and the preparation method of the drynaria extract comprises the following steps: taking a whole plant of drynaria, crushing it, adding water of 8 times the mass of the whole plant of drynaria, adjusting the pH to 5.5, adding hemicellulase and pectinase of 0.5% by mass of the whole plant of drynaria, enzymolysis at 55°C for 3 hours, adjusting the pH to 7.5, adding bromelain and papain of 0.5% by mass of the whole plant of drynaria, enzymolysis at 60°C for 1 hour, heating to 90°C to inactivate the enzyme for 10 minutes, cooling down, adjusting the pH to 8.5, adding nattokinase of 1.0% by mass of the whole plant of drynaria, enzymolysis at 45°C for 1.5 hours, heating to 90°C to inactivate the enzyme for 10 minutes, cooling down, filtering with a 325-mesh sieve, taking the filtrate, and freeze-drying to obtain the drynaria extract.

[0061] The Polygonum multiflorum is a Polygonum multiflorum extract, and the preparation method of the Polygonum multiflorum extract comprises the following steps: taking Polygonum multiflorum tubers, crushing them, adding water of 8 times the mass of Polygonum multiflorum tubers, adjusting the pH to 5.5, adding hemicellulase and pectinase of 0.5% by mass of Polygonum multiflorum tubers, enzymolysis at 55 DEG C for 3 hours, adjusting the pH to 7.5, adding bromelain and papain of 0.5% by mass of Polygonum multiflorum tubers, enzymolysis at 60 DEG C for 1 hour, heating to 90 DEG C to inactivate the enzyme for 10 minutes, cooling down, adjusting the pH to 8.0, adding 0.3% of the mass of Polygonum multiflorum tubers, enzymolysis at 38 DEG C for 1 hour, heating to 90 DEG C to inactivate the enzyme for 10 minutes, cooling down, filtering with a 325-mesh sieve, taking the filtrate, and freeze-drying to obtain the Polygonum multiflorum extract.

[0062] The invention discloses a method for preparing the extract of polygonatum sibiricum, comprising the steps of: taking rhizomes of polygonatum sibiricum, crushing them, adding water 10 times the weight of the rhizomes of polygonatum sibiricum, adjusting the pH to 5.5, adding hemicellulase and pectinase 0.5% by weight of the rhizomes of polygonatum sibiricum, performing enzymolysis at 55°C for 3h, adjusting the pH to 7.5, adding bromelain and papain 0.5% by weight of the rhizomes of polygonatum sibiricum, performing enzymolysis at 60°C for 1h, heating to 90°C to inactivate the enzymes for 10min, cooling to room temperature, and obtaining an enzymolysis solution; and adding the enzymolysis solution to the rhizomes of polygonatum sibiricum. 5% of glucose by weight of the enzymatic hydrolyzate, 0.1% of disodium hydrogen phosphate by weight of the enzymatic hydrolyzate, and 2.5% of ammonium chloride by weight of the enzymatic hydrolyzate were added to the liquid to adjust the pH value to 6.5, and 2% of Bacillus licheniformis by weight of the rhizome of Polygonatum sibiricum was added, and aerobic fermentation was carried out at 30°C for 15 hours. After that, the pH value was adjusted to 4.5, and 2% of Saccharomyces boulardii by weight of the rhizome of Polygonatum sibiricum was added, and anaerobically fermentation was carried out at 36°C for 15 hours, and high-temperature sterilization was carried out at 121°C for 25 minutes. After cooling, filtering was carried out using a 250-mesh sieve, and the filtrate was taken and freeze-dried to obtain a Polygonatum sibiricum extract.

[0063] The preparation method of the above-mentioned Yishen Bugu oral liquid comprises the following steps:

[0064] According to the weight proportions, drynaria extract, polygonum multiflorum extract, poria extract, dipsaci extract, white peony extract, angelica extract, codonopsis extract, rehmannia root extract, polygonatum sibiricum extract, wolfberry fruit extract, natural copper and tangerine peel extract are uniformly mixed to obtain a mixture; the mixture is mixed with sucrose, benzoic acid, ethylparaben and liquor to prepare an oral liquid for nourishing the kidney and bones.

[0065] The enzymatic activity of the hemicellulase used in the above embodiments is 100,000 U / g; the enzymatic activity of the pectinase is 100,000 U / g; the enzymatic activity of the bromelain is 200,000 U / g; the enzymatic activity of the papain is 100,000 U / g; the enzymatic activity of the nattokinase is 20,000 U / g; the enzymatic activity of the protease is 500,000 U / g, which is derived from pigs; the bacterial activity of the Bacillus licheniformis is 20 billion CFU / g; and the bacterial activity of the Saccharomyces boulardii is 20 billion CFU / g.

[0066] Comparative Example 1

[0067] The drynaria extract is an extract obtained by crushing the whole drynaria plant and then percolating it with ethanol (taking the ethanol percolation liquid and freeze-drying it to obtain the extract); other parameters and methods are the same as those in Example 1.

[0068] Comparative Example 2

[0069] In the preparation method of the Rhizoma Drynariae extract, nattokinase is not used for enzymatic hydrolysis; other parameters and methods are the same as those in Example 1.

[0070] Comparative Example 3

[0071] The Polygonum multiflorum root is an extract obtained by crushing the Polygonum multiflorum root tuber and then percolating it with ethanol (taking the ethanol percolation liquid and freeze-drying it to obtain the extract); other parameters and methods are the same as those in Example 1.

[0072] Comparative Example 4

[0073] In the preparation method of the Polygonum multiflorum extract, trypsin is not used for enzymatic hydrolysis; other parameters and methods are the same as those in Example 1.

[0074] Comparative Example 5

[0075] The polygonatum sibiricum is an extract obtained by crushing the rhizome of polygonatum sibiricum and then percolating it with ethanol (taking the ethanol percolation liquid and freeze-drying it to obtain the extract); other parameters and methods are the same as those in Example 1.

[0076] Comparative Example 6

[0077] In the preparation method of the polygonatum sibiricum extract, fermentation with Bacillus licheniformis and Saccharomyces boulardii is not performed; other parameters and methods are the same as those in Example 1.

[0078] Comparative Example 7

[0079] In the preparation method of the polygonatum sibiricum extract, enzymolysis by bromelain and papain is not performed; other parameters and methods are the same as those in Example 1.

[0080] Comparative Example 8

[0081] Drynaria Rhizoma, Polygonum Multiflorum and Polygonatum Sinensis are all extracts obtained by ethanol percolation of traditional Chinese medicine dry powder (the ethanol percolation liquid is taken and freeze-dried to obtain the extract); other parameters and methods are the same as those in Example 1.

[0082] Safety testing was performed on the main raw material components prepared in the above embodiments and comparative examples.

[0083] According to the conventional detection method, the acute oral toxicity test was conducted on mice, and 0.5g of the main raw material component was gavage, which was non-toxic; the mouse bone marrow cell micronucleus test and the mouse sperm deformity test were all negative; the rat feeding test for 60 days was conducted on rats, and the rats were fed with 0.5g / kg·bw / d, 1g / kg·bw / d, and 2g / kg·bw / d of the main raw material component. The rats grew and developed well, and there were no abnormal changes in body weight, organ weight, and organ coefficient in each dosage group.

[0084] The oral liquids prepared in the above-mentioned embodiments and comparative examples were tested for their kidney-tonifying and bone-strengthening efficacy.

[0085] 1. Experiment on improving renal function

[0086] 112 subjects (30 to 60 years old) were selected and randomly divided into 14 groups, with 8 subjects in each group (half male and half female). Blood samples were taken before the test for detection of renal function-related indices. After obtaining the initial values, one group of subjects did not take the oral solution as a blank control group and had a normal diet and life; the remaining 13 groups of subjects took the oral solution of each embodiment and comparative example, 15 mL per day, and had a normal diet and life; after 50 days, blood samples were taken for detection of renal-related indices.

[0087] Renal function index detection: Sampling was performed at 8 am before the test and 50 days later, and venous blood sampling was performed (fasting state was required for 8 hours before blood collection), and the samples were tested for Scr (serum creatinine) and BUN (urea nitrogen) indexes. Blood samples were obtained by venous blood collection, and the collected blood samples were centrifuged to separate serum, and tested by fully automatic biochemical analyzer and picric acid method. Based on the reaction of creatinine and picric acid under alkaline conditions to generate orange-red picric acid creatinine complex, the Scr content was determined by colorimetry; similarly, after the blood samples were processed, they were tested by fully automatic biochemical analyzer, and the urease method was used to determine the BUN content. The urease method uses urease to catalyze the hydrolysis of urea to produce ammonia and carbon dioxide, and then the concentration of urea nitrogen is determined by detecting the ammonia content; the Scr and BUN indicators were tested before and after the test respectively; the change rate of Scr and BUN was calculated. Change rate = (content after the test - content before the test) / content before the test; the test results are shown in Table 1 below.

[0088] Table 1 Renal function test results (mean values)

[0089]

[0090]

[0091] In the rate of change, “+” indicates an increase, and “-” indicates a decrease.

[0092] The worse the kidney function is, the higher the blood creatinine (Scr) and urea nitrogen (BUN) will usually be. Blood creatinine is a product of muscle metabolism and is mainly excreted through the kidneys. When kidney function declines, the kidney's ability to excrete blood creatinine decreases, causing blood creatinine to accumulate in the blood and increase in its content. Urea nitrogen is the end product of protein metabolism and is also mainly excreted through the kidneys. When kidney function is poor, the excretion of urea nitrogen decreases and the urea nitrogen content in the blood increases.

[0093] As can be seen from the above results, the oral liquids of Examples 1 to 5 have a good effect on improving kidney function, and quickly and effectively reduce the content of blood creatinine and urea nitrogen in the blood. As can be seen from the results of Comparative Examples 1 and 2, changing the preparation method of the Drynaria extract, the obtained product components are different, which will reduce the effect of improving kidney metabolism regulation, indicating that the product obtained by the Drynaria extract using a specific enzymatic hydrolysis step has a more effective and rapid kidney metabolism regulation and improvement effect. As can be seen from the results of Comparative Examples 3 and 4, changing the preparation method of the Polygonum multiflorum extract, the obtained product components are different, which will reduce the effect of improving kidney metabolism regulation, indicating that the product obtained by the Polygonum multiflorum extract using a specific enzymatic hydrolysis step has a more effective and rapid kidney metabolism regulation and improvement effect. As can be seen from the results of Comparative Examples 5 and 7, changing the preparation method of the Polygonatum sibiricum extract, the obtained product components are different, which will reduce the effect of improving kidney metabolism regulation, indicating that the product obtained by the Polygonatum sibiricum extract using a specific enzymatic hydrolysis step has a more effective and rapid kidney metabolism regulation and improvement effect. From the results of Comparative Example 8, it can be seen that the conventional extraction method, in which Rhizoma Drynariae, Polygonum Multiflorum and Polygonatum sibiricum are all extracts obtained by ethanol percolation of Chinese medicine dry powder, will greatly reduce the efficacy of the oral solution, the effect is not obvious, and the treatment course is long.

[0094] 2. Animal Experiments on Increasing Bone Density

[0095] 150 8-month-old female rats of Kunming strain (weight 263g-275g) were selected and randomly divided into groups: 1 sham operation group (10 rats), 1 ovariectomy blank group (10 rats), and 13 ovariectomy test groups (10 rats in each group, taking the oral liquid of each embodiment and each comparative example, respectively). Among them, the ovariectomy blank group and the ovariectomy test group were bilateral ovariectomy rats, and the sham operation group was only skin incision without ovariectomy. After the operation, all rats ate and drank water normally every day, among which, the ovariectomy test group rats were also gavaged with 2.5ml of oral liquid every day (each group took the oral liquid of each embodiment and each comparative example, respectively); after 50 days of continuous feeding, the bone density of the lumbar vertebrae and left femur of each rat was detected by X-ray bone densitometer, and the results are shown in Table 2 below.

[0096] Table 2 Bone density test results (average value)

[0097]

[0098] It can be seen from the above results that the oral liquids of Examples 1 to 5 have a good effect of increasing bone density. It can be seen from the results of Comparative Examples 1 and 2 that changing the preparation method of Drynaria extract, the obtained product components are different, which will reduce the improvement effect of bone density, indicating that the product obtained by using a specific enzymatic hydrolysis step of Drynaria extract has a more effective and rapid bone density improvement effect. It can be seen from the results of Comparative Examples 3 and 4 that changing the preparation method of Polygonum multiflorum extract, the obtained product components are different, which will reduce the improvement of bone density, indicating that the product obtained by using a specific enzymatic hydrolysis step of Polygonum multiflorum extract has a more effective and rapid bone density improvement effect. It can be seen from the results of Comparative Examples 5 and 7 that changing the preparation method of Polygonatum sibiricum extract, the obtained product components are different, which will reduce the improvement effect of bone density, indicating that the product obtained by using a specific enzymatic hydrolysis step of Polygonatum sibiricum extract has a more effective and rapid bone density improvement effect. From the results of Comparative Example 8, it can be seen that the conventional extraction method, in which Rhizoma Drynariae, Polygonum Multiflorum and Polygonatum sibiricum are all extracts obtained by ethanol percolation of Chinese medicine dry powder, will greatly reduce the efficacy of oral liquid in improving bone density, the effect is not obvious, and the treatment course is long.

Claims

1. A kidney-tonifying and bone-nourishing oral liquid, characterized in that: The oral solution comprises the following main raw materials in parts by weight: 5 to 8 parts of Drynaria fortunei, 3 to 6 parts of Polygonum multiflorum, 3 to 6 parts of Poria cocos, 1 to 3 parts of Dipsacus asper, 0.5 to 2 parts of White Peony Root, 0.5 to 1 part of Angelica sinensis, 0.1 to 0.3 parts of Codonopsis pilosula, 0.1 to 0.3 parts of Rehmannia root, 0.1 to 0.3 parts of Polygonatum sibiricum, 0.1 to 0.3 parts of Lycium barbarum, 0.05 to 0.1 parts of Natural Copper, and 0.05 to 0.1 parts of Tangerine peel; The drynaria extract is a drynaria extract, which is an extract obtained by hydrolyzing the whole drynaria plant with hemicellulase and pectinase, then hydrolyzing it with bromelain and papain, and finally hydrolyzing it with nattokinase; The Polygonum multiflorum is a Polygonum multiflorum extract, which is an extract obtained by hydrolyzing the Polygonum multiflorum root tuber with hemicellulase and pectinase, then with bromelain and papain, and finally with trypsin; The polygonatum sibiricum is a polygonatum sibiricum extract, which is an extract obtained by hydrolyzing the rhizome of polygonatum sibiricum with hemicellulase and pectinase, then hydrolyzing it with bromelain and papain, and then fermenting it with Bacillus licheniformis and Saccharomyces boulardii.

2. The oral liquid for nourishing kidney and nourishing bones according to claim 1, characterized in that: The oral liquid also includes the following auxiliary materials in percentage by weight: 5% to 20% of the total weight of the main raw material of sucrose, 0.03% to 0.06% of the total weight of the main raw material of benzoic acid, 0.05% to 0.1% of the total weight of the main raw material of ethylparaben, and 10 to 30 times the total weight of the main raw material of liquor; the alcohol content of the liquor is 3° to 12°.

3. The oral liquid for nourishing kidney and nourishing bones according to claim 1, characterized in that: The preparation method of the drynaria extract comprises the following steps: taking the whole drynaria plant, crushing it, adding water, adjusting the pH to 5.0-5.5, adding hemicellulase and pectinase, enzymolysis at 45-55°C for 3h-4h, adjusting the pH to 6.0-7.5, adding bromelain and papain, enzymolysis at 50-60°C for 1h-1.5h, heating to inactivate the enzyme, cooling down, adjusting the pH to 7.5-8.5, adding nattokinase, enzymolysis at 40-45°C for 1.5h-2h, heating to inactivate the enzyme, cooling down, filtering with a sieve, taking the filtrate, and freeze-drying to obtain the drynaria extract.

4. The oral liquid for nourishing kidney and nourishing bones according to claim 3, characterized in that: The amount of water added is 8 to 12 times the mass of the whole plant of Drynaria; the amount of hemicellulase, pectinase, bromelain and papain added is 0.5% to 2% of the mass of the whole plant of Drynaria; the amount of nattokinase added is 1.0% to 2.5% of the mass of the whole plant of Drynaria; the temperature for heating and inactivating the enzyme is 80°C to 90°C, and the time for heating and inactivating the enzyme is 10 minutes to 20 minutes; the mesh size of the sieve is 250 meshes to 325 meshes.

5. The oral liquid for nourishing kidney and nourishing bones according to claim 1, characterized in that: The preparation method of the Polygonum multiflorum extract comprises the following steps: taking Polygonum multiflorum root tubers, crushing them, adding water, adjusting the pH to 5.0-5.5, adding hemicellulase and pectinase, performing enzymolysis at 45-55°C for 3h-4h, adjusting the pH to 6.0-7.5, adding bromelain and papain, performing enzymolysis at 50-60°C for 1h-1.5h, heating up to inactivate the enzymes, cooling down, adjusting the pH to 7.0-8.0, adding protease, performing enzymolysis at 36-38°C for 1h-1.5h, heating up to inactivate the enzymes, cooling down, filtering with a sieve, taking the filtrate, and freeze-drying to obtain the Polygonum multiflorum extract.

6. The oral liquid for nourishing kidney and nourishing bones according to claim 5, characterized in that: The amount of water added is 8 to 12 times the mass of the Polygonum multiflorum root tuber; the amount of hemicellulase, pectinase, bromelain and papain added is 0.5% to 2% of the mass of the Polygonum multiflorum root tuber; the amount of protease added is 0.3% to 1.0% of the mass of the Polygonum multiflorum root tuber; the temperature for heating and inactivating the enzyme is 80°C to 90°C, and the time for heating and inactivating the enzyme is 10min to 20min; the mesh size of the sieve is 250 to 325 meshes.

7. The oral liquid for nourishing kidney and nourishing bones according to claim 1, characterized in that: The preparation method of the polygonatum sibiricum extract comprises the following steps: taking polygonatum sibiricum rhizome, crushing it, adding water, adjusting the pH to 5.0-5.5, adding hemicellulase and pectinase, performing enzymolysis at 45-55°C for 3h-4h, adjusting the pH to 6.0-7.5, adding bromelain and papain, performing enzymolysis at 50-60°C for 1h-1.5h, heating to inactivate the enzymes, cooling to room temperature, and obtaining an enzymolysis solution; adding glucose, disodium hydrogen phosphate, and ammonium chloride to the enzymolysis solution, adjusting the pH to 6.5-7.5, adding Bacillus licheniformis, performing aerobic fermentation at 30-37°C for 12h-15h, then adjusting the pH to 4.5-5.0, adding Saccharomyces boulardii, performing anaerobically fermentation at 36-38°C for 12h-15h, sterilizing at high temperature, cooling, filtering with a sieve, taking the filtrate, and freeze-drying to obtain the polygonatum sibiricum extract.

8. The oral liquid for nourishing kidney and nourishing bones according to claim 8, characterized in that: The amount of water added is 10 to 15 times the mass of the rhizome of polygonatum; the amount of hemicellulase, pectinase, bromelain and papain added is 0.5% to 2% of the mass of the rhizome of polygonatum; the amount of glucose added is 3% to 5% of the mass of the enzymatic solution; the amount of disodium hydrogen phosphate added is 0.1% to 0.5% of the mass of the enzymatic solution; the amount of ammonium chloride added is 1.5% to 2.5% of the mass of the enzymatic solution; the amount of Bacillus licheniformis and Saccharomyces boulardii added is 0.5% to 2% of the mass of the rhizome of polygonatum; the temperature of heating to inactivate the enzyme is 80°C to 90°C, and the time of heating to inactivate the enzyme is 10min to 20min; the temperature of high-temperature sterilization is 121°C to 125°C, and the time of high-temperature sterilization is 15min to 25min; the mesh size of the sieve is 250 mesh to 325 mesh.

9. The oral liquid for nourishing kidney and nourishing bones according to claim 1, characterized in that: The Poria, Dipsacus asper, White Peony Root, Angelica sinensis, Codonopsis pilosula, Rehmannia root, Fructus Lycii and Citrus reticulatae are all extracts obtained by ethanol percolation of Chinese medicine dry powder.

10. A method for preparing a Yishen Bugu oral liquid, used for preparing the Yishen Bugu oral liquid according to claim 1, characterized in that: The preparation method comprises the following steps: According to the weight proportions, drynaria root, polygonum multiflorum, poria, dipsaci, white peony root, angelica, codonopsis, rehmannia root, polygonatum sibiricum, wolfberry, natural copper and tangerine peel are uniformly mixed to obtain a mixture; the mixture is mixed with preservatives, flavoring agents and solvents which are allowed to be added to the oral liquid of the medicine to prepare an oral liquid for nourishing the kidney and bones.

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