Eye protection solution based on natural extracts and preparation method thereof

Abstract

The invention discloses an eye protection liquid based on natural extracts and a preparation method thereof, which belongs to the technical field of eye protection liquids, and comprises the following raw materials in parts by weight: 100-200 parts of 0.9% NaCl solution, 12-14 parts of sodium hyaluronate, 1-3 parts of lutein, 0.1-1 parts of natural borneol powder, 16-25 parts of fermented extracts of cassia seed and wild chrysanthemum, 17-22 parts of eggshell membrane extract, and 6-10 parts of triterpenes of Rosa laevigata root. Through the above-mentioned method, the eye protection liquid of the invention is prepared by using sodium hyaluronate, lutein, natural borneol powder, water extracts of cassia seed and wild chrysanthemum, eggshell membrane extract, and triterpenes of Rosa laevigata root, and has fewer types of raw materials, does not contain alcohol, has lower irritation, is suitable for people with sensitive eyes, has a simple method of use, and can effectively improve dry eye syndrome.

Description

Technical Field

[0001] The invention relates to the technical field of eye protection liquid, and in particular to an eye protection liquid based on natural extracts and a preparation method thereof. Background Art

[0002] Eye protection solution can be used when the eyes are overused due to long-term use of electronic products, study, work, etc. It can also be used as a daily care product for those wearing cosmetic contact lenses, etc., and can effectively relieve eye fatigue.

[0003] In order to alleviate the side effects caused by long-term use of eye drops containing chemical ingredients, some pure plant-based eye drops have appeared on the market. For example, Chinese invention patent CN111558031B discloses a pure plant-based multifunctional eye nutrient solution and its preparation method, which includes the following raw materials: angelica peptide, ginseng peptide, sea cucumber peptide, bitter melon peptide, astragalus, rhodiola rosea, houttuynia cordata, peach kernel, safflower, kudzu root, adenophora, Scrophularia ningpoensis, salvia miltiorrhiza, wine rhubarb, herba schizonepetae and mother of pearl.

[0004] However, it has the following problems:

[0005] 1) There are many kinds of raw materials, which are irritating to the eyes and are not suitable for people with sensitive eyes (such as children, the elderly, etc.);

[0006] 2) It uses 55°C high-proof grain liquor to turn borneol into liquid. The addition of alcohol will further increase the irritation of the product and even cause allergic reactions.

[0007] Based on this, the present invention designs an eye protection solution based on natural extracts and a preparation method thereof to solve the above problems. Summary of the invention

[0008] In view of the above-mentioned shortcomings of the prior art, the present invention provides an eye protection solution based on natural extracts and a preparation method thereof.

[0009] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0010] An eye protection solution based on natural extracts, comprising the following raw materials in parts by weight: 100-200 parts of 0.9% NaCl solution, 12-14 parts of sodium hyaluronate, 1-3 parts of lutein, 0.1-1 parts of natural borneol powder, 16-25 parts of fermented extracts of cassia seeds and wild chrysanthemums, 17-22 parts of eggshell membrane extracts, and 6-10 parts of triterpenes from the root of Rosa laevigata;

[0011] The method for preparing the eggshell membrane extract comprises the following steps:

[0012] A. Collect eggshell membranes, crush them, add 2-3 times the volume of 0.6% saline, and soak for 15-20 minutes;

[0013] B. Take out the eggshell membrane powder and immerse it in acetic anhydride solution for 4-6 minutes; then immerse it in gelatinized starch solution containing coupling agent, and irradiate it at the same time, the irradiation dose is 0.35-0.42 kGy, and the irradiation time is 10-15 minutes;

[0014] C. Filter out the eggshell membrane powder, wash it with water 1-3 times, first hydrolyze it with pepsin (pH 2, 37°C, 5h), then hydrolyze it with alkaline protease (pH 9, 53°C, 1.5h), and add 7-8U / mg of enzyme;

[0015] D. Ultrafiltration to obtain ultrafiltration separation components with a molecular weight of 5~10kDa.

[0016] Furthermore, the eye protection solution is packaged in a spray bottle.

[0017] Furthermore, the mass ratio of cassia seed to wild chrysanthemum in the fermentation extract of cassia seed and wild chrysanthemum is 1.5-2:1.

[0018] Furthermore, the particle size of the natural borneol powder is nanometer scale.

[0019] Furthermore, the concentration of the acetic anhydride solution is 1-1.3 mol / L.

[0020] Furthermore, the coupling agent is an amino-terminated silane coupling agent.

[0021] Furthermore, the gelatinized starch solution is prepared by mixing gelatinized starch with 4 to 8 times the volume of water.

[0022] Furthermore, the method for preparing the extract by fermenting Cassia seed and wild chrysanthemum is as follows:

[0023] A. Wash Cassia seed and wild chrysanthemum, dry them in the sun, freeze them at a low temperature below -25°C and grind them into mixed plant powder;

[0024] B. Add water and yeast to the plant mixed powder, mix well, ferment at 25-30℃ for 4-6h, then steam at 100-105℃ for 5-8min, take out and cool to 35-38℃ while stirring, then add lactic acid bacteria, mix well, ferment at 35-38℃ for 1-2h, then steam at 95-100℃ for 1-3min;

[0025] C. Ultrasonic assisted extraction after centrifugation, with an ultrasonic power of 300-400 W, an ultrasonic frequency of 30-40 kHz, and a time of 25-30 min, followed by microfiltration, with a filter membrane pore size of 0.6-0.8 μm to obtain a filtrate;

[0026] D. Adjust the pH of the filtrate to 7-7.2, extract with an equal volume of ethanol, recover the ethanol by vacuum distillation, and then filter with a filter membrane with a pore size of 0.5-0.7 μm to obtain a clear liquid, which is the fermentation and extract of Cassia seed and wild chrysanthemum.

[0027] Furthermore, the triterpene compound of the root of Rosa laevigata is Rosa laevigata sapogenin.

[0028] A method for preparing an eye protection liquid based on natural extracts comprises the following steps: adding sodium hyaluronate, lutein, natural borneol powder, fermented extracts of cassia seeds and wild chrysanthemums, eggshell membrane extracts, and triterpenes from the root of Rosa laevigata to a 0.9% NaCl solution, stirring evenly at 2000-3000 r / min, and sterilizing to obtain the eye protection liquid based on natural extracts.

[0029] In order to better achieve the purpose of the present invention, the present invention also provides an eye protection solution based on natural extracts and a preparation method thereof.

[0030] Compared with the prior art, the present invention has the following beneficial effects: the eye protection liquid of the present invention is prepared by using sodium hyaluronate, lutein, natural borneol powder, cassia seed and wild chrysanthemum water extract, eggshell membrane extract, and Rosa laevigata root triterpenoids, has fewer types of raw materials, does not contain alcohol, has lower irritation, is suitable for people with sensitive eyes, has a simple method of use, and can effectively improve dry eye syndrome. DETAILED DESCRIPTION

[0031] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] Embodiment 1: In some embodiments, a method for preparing an eye protection solution based on natural extracts comprises the following steps:

[0033] 1. Preparation of eggshell membrane extract:

[0034] A. Collect eggshell membranes, crush them, add 2 times the volume of 0.6% saline, and soak for 20 minutes;

[0035] B. Take out the eggshell membrane powder and immerse it in acetic anhydride solution (1 mol / L) for 6 minutes; then immerse it in gelatinized starch solution (prepared by mixing gelatinized starch with 4 times the volume of water) containing a coupling agent (terminated amino silane coupling agent), and irradiate it at the same time, with an irradiation dose of 0.42 kGy and an irradiation time of 10 minutes;

[0036] C. Filter out the eggshell membrane powder, wash it once with clean water, first hydrolyze it with pepsin (pH 2, 37°C, 5h), then hydrolyze it with alkaline protease (pH 9, 53°C, 1.5h), and add 8U / mg of enzyme;

[0037] D. Ultrafiltration to obtain ultrafiltration separation components with a molecular weight of 5~10kDa.

[0038] 2. Preparation of fermentation and extract of Cassia seed and wild chrysanthemum:

[0039] A. Wash Cassia seed and wild chrysanthemum, dry them in the sun, freeze them at a low temperature below -25°C and grind them into mixed plant powder;

[0040] B. Add water and yeast to the plant mixed powder, mix well, ferment at 25°C for 6 hours, then steam at 100°C for 8 minutes, take out and cool to 35°C while stirring, then add lactic acid bacteria, mix well, ferment at 35°C for 1 hour, then steam at 100°C for 1 minute;

[0041] C. Ultrasonic assisted extraction after centrifugation, with an ultrasonic power of 300 W, an ultrasonic frequency of 40 kHz, and a time of 25 min, followed by microfiltration, with a filter membrane pore size of 0.8 μm to obtain a filtrate;

[0042] D. Adjust the pH of the filtrate to 7, extract with an equal volume of ethanol, recover the ethanol by vacuum distillation, and then filter with a filter membrane with a pore size of 0.7 μm to obtain a clear liquid, which is the fermentation and extract of Cassia seed and wild chrysanthemum.

[0043] 3. Weigh the raw materials: 100 g of 0.9% NaCl solution, 14 g of sodium hyaluronate, 1 g of lutein, 1 g of natural borneol powder (nanoscale), 16 g of fermented extract of cassia seed and wild chrysanthemum (the mass ratio of cassia seed: wild chrysanthemum is 2:1), 22 g of eggshell membrane extract, 6 g of triterpenoids from the root of Rosa laevigata (Rosa laevigata sapogenin, for the preparation method, refer to 201810455214.6).

[0044] 4. Add sodium hyaluronate, lutein, natural borneol powder, fermentation and extract of cassia seed and wild chrysanthemum, eggshell membrane extract, and triterpenoids from the root of Rosa laevigata to 0.9% NaCl solution, stir evenly at 2000 r / min, and sterilize to obtain an eye protection solution based on natural extracts.

[0045] Embodiment 2: In some embodiments, a method for preparing an eye protection solution based on natural extracts comprises the following steps:

[0046] 1. Preparation of eggshell membrane extract:

[0047] A. Collect eggshell membranes, crush them, add 3 times the volume of 0.6% saline, and soak for 15 minutes;

[0048] B. Take out the eggshell membrane powder and immerse it in acetic anhydride solution (1.3 mol / L) for 4 min; then immerse it in gelatinized starch solution (prepared by mixing gelatinized starch with 8 times volume of water) containing coupling agent (terminated amino silane coupling agent), and irradiate it at the same time, with an irradiation dose of 0.35 kGy and an irradiation time of 15 min;

[0049] C. Filter out the eggshell membrane powder, wash it with water three times, first hydrolyze it with pepsin (pH 2, 37°C, 5h), then hydrolyze it with alkaline protease (pH 9, 53°C, 1.5h), and add 7U / mg of enzyme;

[0050] D. Ultrafiltration to obtain ultrafiltration separation components with a molecular weight of 5~10kDa.

[0051] 2. Preparation of fermentation and extract of Cassia seed and wild chrysanthemum:

[0052] A. Wash Cassia seed and wild chrysanthemum, dry them in the sun, freeze them at a low temperature below -25°C and grind them into mixed plant powder;

[0053] B. Add water and yeast to the plant mixed powder, mix well, ferment at 30°C for 4 hours, then steam at 105°C for 5 minutes, take out and cool to 38°C while stirring, then add lactic acid bacteria, mix well, ferment at 38°C for 2 hours, then steam at 95°C for 3 minutes;

[0054] C. Ultrasonic assisted extraction after centrifugation, with an ultrasonic power of 400 W, an ultrasonic frequency of 30 kHz, and a time of 30 min, followed by microfiltration, with a filter membrane pore size of 0.6 μm to obtain a filtrate;

[0055] D. Adjust the pH of the filtrate to 7.2, extract with an equal volume of ethanol, recover the ethanol by vacuum distillation, and then filter with a filter membrane with a pore size of 0.5 μm to obtain a clear liquid, which is the fermentation and extract of Cassia seed and wild chrysanthemum.

[0056] 3. Weigh the raw materials: 200 g of 0.9% NaCl solution, 12 g of sodium hyaluronate, 3 g of lutein, 0.1 g of natural borneol powder (nanoscale), 25 g of fermented extract of cassia seed and wild chrysanthemum (the mass ratio of cassia seed: wild chrysanthemum is 1.5:1), 17 g of eggshell membrane extract, 10 g of triterpenoids from the root of Rosa laevigata (Rosa laevigata sapogenin, for the preparation method, refer to 201810455214.6).

[0057] 4. Add sodium hyaluronate, lutein, natural borneol powder, fermentation and extract of cassia seed and wild chrysanthemum, eggshell membrane extract, and triterpenoids from the root of Rosa laevigata to 0.9% NaCl solution, stir evenly at 3000 r / min, and sterilize to obtain an eye protection solution based on natural extracts.

[0058] Embodiment 3: In some embodiments, a method for preparing an eye protection solution based on natural extracts comprises the following steps:

[0059] 1. Preparation of eggshell membrane extract:

[0060] A. Collect eggshell membranes, crush them, add 2.2 times the volume of 0.6% saline, and soak for 16 minutes;

[0061] B. After taking out the eggshell membrane powder, immerse it in an acetic anhydride solution (1.1 mol / L) for 5 min; then immerse it in a gelatinized starch solution (prepared by mixing gelatinized starch with 5 times the volume of water) containing a coupling agent (terminated amino silane coupling agent), and irradiate it at the same time, with an irradiation dose of 0.38 kGy and an irradiation time of 11 min;

[0062] C. Filter out the eggshell membrane powder, wash it twice with water, first hydrolyze it with pepsin (pH 2, 37°C, 5h), then hydrolyze it with alkaline protease (pH 9, 53°C, 1.5h), and add 7.5U / mg of enzyme;

[0063] D. Ultrafiltration to obtain ultrafiltration separation components with a molecular weight of 5~10kDa.

[0064] 2. Preparation of fermentation and extract of Cassia seed and wild chrysanthemum:

[0065] A. Wash Cassia seed and wild chrysanthemum, dry them in the sun, freeze them at a low temperature below -25°C and grind them into mixed plant powder;

[0066] B. Add water and yeast to the plant mixed powder, mix well, ferment at 26°C for 5 hours, then steam at 102°C for 6 minutes, take out and cool to 36°C while stirring, then add lactic acid bacteria, mix well, ferment at 36°C for 1.5 hours, then steam at 98°C for 2 minutes;

[0067] C. Ultrasonic assisted extraction after centrifugation, with an ultrasonic power of 350 W, an ultrasonic frequency of 33 kHz, and a time of 26 min, followed by microfiltration, with a filter membrane pore size of 0.7 μm to obtain a filtrate;

[0068] D. Adjust the pH of the filtrate to 7.1, extract with an equal volume of ethanol, recover the ethanol by vacuum distillation, and then filter with a filter membrane with a pore size of 0.6 μm to obtain a clear liquid, which is the fermentation and extract of Cassia seed and wild chrysanthemum.

[0069] 3. Weigh the raw materials: 120g of 0.9% NaCl solution, 13g of sodium hyaluronate, 1.3g of lutein, 0.2g of natural borneol powder (nanoscale), 18g of fermented extract of cassia seed and wild chrysanthemum (the mass ratio of cassia seed: wild chrysanthemum is 1.8:1), 18g of eggshell membrane extract, 7g of triterpenoids from the root of Rosa laevigata (Rosa laevigata sapogenin, for the preparation method, see 201810455214.6).

[0070] 4. Add sodium hyaluronate, lutein, natural borneol powder, fermentation and extract of cassia seed and wild chrysanthemum, eggshell membrane extract, and triterpenoids from the root of Rosa laevigata to 0.9% NaCl solution, stir evenly at 2500r / min, and sterilize to obtain an eye protection solution based on natural extracts.

[0071] Embodiment 4: In some embodiments, a method for preparing an eye protection solution based on natural extracts comprises the following steps:

[0072] 1. Preparation of eggshell membrane extract:

[0073] A. Collect eggshell membranes, crush them, add 2 times the volume of 0.6% saline, and soak for 18 minutes;

[0074] B. After the eggshell membrane powder is taken out, it is immersed in an acetic anhydride solution (1 mol / L) for 6 minutes; then immersed in a gelatinized starch solution (prepared by mixing gelatinized starch with 7 times the volume of water) containing a coupling agent (terminated amino silane coupling agent), and irradiated at the same time, with an irradiation dose of 0.40 kGy and an irradiation time of 13 minutes;

[0075] C. Filter out the eggshell membrane powder, wash it with water three times, first hydrolyze it with pepsin (pH 2, 37°C, 5h), then hydrolyze it with alkaline protease (pH 9, 53°C, 1.5h), and add 7U / mg of enzyme;

[0076] D. Ultrafiltration to obtain ultrafiltration separation components with a molecular weight of 5~10kDa.

[0077] 2. Preparation of fermentation and extract of Cassia seed and wild chrysanthemum:

[0078] A. Wash Cassia seed and wild chrysanthemum, dry them in the sun, freeze them at a low temperature below -25°C and grind them into mixed plant powder;

[0079] B. Add water and yeast to the plant mixed powder, mix well, ferment at 29°C for 5 hours, then steam at 104°C for 7 minutes, take out and cool to 37°C while stirring, then add lactic acid bacteria, mix well, ferment at 37°C for 1 hour, then steam at 99°C for 2 minutes;

[0080] C. Ultrasonic assisted extraction after centrifugation, with an ultrasonic power of 340 W, an ultrasonic frequency of 35 kHz, and a time of 29 min, followed by microfiltration, with a filter membrane pore size of 0.7 μm to obtain a filtrate;

[0081] D. Adjust the pH of the filtrate to 7, extract with an equal volume of ethanol, recover the ethanol by vacuum distillation, and then filter with a filter membrane with a pore size of 0.6 μm to obtain a clear liquid, which is the fermentation and extract of Cassia seed and wild chrysanthemum.

[0082] 3. Weigh the raw materials: 140g of 0.9% NaCl solution, 13.5g of sodium hyaluronate, 2.2g of lutein, 0.3g of natural borneol powder (nanoscale), 19g of fermentation and extract of cassia seed and wild chrysanthemum (the mass ratio of cassia seed: wild chrysanthemum is 1.8:1), 20g of eggshell membrane extract, 8g of triterpenoids from the root of Rosa laevigata (Rosa laevigata sapogenin, for the preparation method, refer to 201810455214.6).

[0083] 4. Add sodium hyaluronate, lutein, natural borneol powder, fermentation and extract of cassia seed and wild chrysanthemum, eggshell membrane extract, and triterpenoids from the root of Rosa laevigata to 0.9% NaCl solution, stir evenly at 2700 r / min, and sterilize to obtain an eye protection solution based on natural extracts.

[0084] Embodiment 5: In some embodiments, a method for preparing an eye protection solution based on natural extracts comprises the following steps:

[0085] 1. Preparation of eggshell membrane extract:

[0086] A. Collect eggshell membranes, crush them, add 3 times the volume of 0.6% saline, and soak for 19 minutes;

[0087] B. Take out the eggshell membrane powder and immerse it in acetic anhydride solution (1 mol / L) for 4.5 min; then immerse it in gelatinized starch solution (prepared by mixing gelatinized starch with 7 times volume of water) containing coupling agent (terminated amino silane coupling agent), and irradiate it at the same time, with an irradiation dose of 0.41 kGy and an irradiation time of 13 min;

[0088] C. Filter out the eggshell membrane powder, wash it twice with water, first hydrolyze it with pepsin (pH 2, 37°C, 5h), then hydrolyze it with alkaline protease (pH 9, 53°C, 1.5h), and add 7.5U / mg of enzyme;

[0089] D. Ultrafiltration to obtain ultrafiltration separation components with a molecular weight of 5~10kDa.

[0090] 2. Preparation of fermentation and extract of Cassia seed and wild chrysanthemum:

[0091] A. Wash Cassia seed and wild chrysanthemum, dry them in the sun, freeze them at a low temperature below -25°C and grind them into mixed plant powder;

[0092] B. Add water and yeast to the plant mixed powder, mix well, ferment at 26°C for 5 hours, and then steam at 103°C for 7 minutes. Take it out and cool it to 35.5°C while stirring. Then add lactic acid bacteria, mix well, ferment at 35.5°C for 1.6 hours, and then steam at 98°C for 2 minutes.

[0093] C. Ultrasonic assisted extraction after centrifugation, with an ultrasonic power of 380 W, an ultrasonic frequency of 39 kHz, and a time of 27 min, followed by microfiltration, with a filter membrane pore size of 0.7 μm to obtain a filtrate;

[0094] D. Adjust the pH of the filtrate to 7.2, extract with an equal volume of ethanol, recover the ethanol by vacuum distillation, and then filter with a filter membrane with a pore size of 0.6 μm to obtain a clear liquid, which is the fermentation and extract of Cassia seed and wild chrysanthemum.

[0095] 3. Weigh the raw materials: 170g of 0.9% NaCl solution, 13g of sodium hyaluronate, 2.3g of lutein, 0.7g of natural borneol powder (nanoscale), 22g of fermented extract of cassia seed and wild chrysanthemum (the mass ratio of cassia seed: wild chrysanthemum is 1.8:1), 21g of eggshell membrane extract, 8g of triterpenoids from the root of Rosa laevigata (Rosa laevigata sapogenin, for the preparation method, see 201810455214.6).

[0096] 4. Add sodium hyaluronate, lutein, natural borneol powder, fermentation and extract of cassia seed and wild chrysanthemum, eggshell membrane extract, and triterpenoids from the root of Rosa laevigata to 0.9% NaCl solution, stir evenly at 2600 r / min, and sterilize to obtain an eye protection solution based on natural extracts.

[0097] Comparative Example 1: The difference from Example 5 is that the eggshell membrane extract is directly subjected to enzymatic hydrolysis during its preparation process, and the preparation method comprises the following steps:

[0098] 1. Preparation of eggshell membrane extract:

[0099] A. Collect eggshell membranes, crush them, wash them twice with water, and first hydrolyze them with pepsin (pH 2, 37°C, 5h), then with alkaline protease (pH 9, 53°C, 1.5h), with the enzyme addition amount of 7.5U / mg;

[0100] B. Ultrafiltration to obtain ultrafiltration separation components with a molecular weight of 5~10kDa.

[0101] 2. Preparation of fermentation and extract of Cassia seed and wild chrysanthemum:

[0102] A. Wash Cassia seed and wild chrysanthemum, dry them in the sun, freeze them at a low temperature below -25°C and grind them into mixed plant powder;

[0103] B. Add water and yeast to the plant mixed powder, mix well, ferment at 26°C for 5 hours, and then steam at 103°C for 7 minutes. Take it out and cool it to 35.5°C while stirring. Then add lactic acid bacteria, mix well, ferment at 35.5°C for 1.6 hours, and then steam at 98°C for 2 minutes.

[0104] C. Ultrasonic assisted extraction after centrifugation, with an ultrasonic power of 380 W, an ultrasonic frequency of 39 kHz, and a time of 27 min, followed by microfiltration, with a filter membrane pore size of 0.7 μm to obtain a filtrate;

[0105] D. Adjust the pH of the filtrate to 7.2, extract with an equal volume of ethanol, recover the ethanol by vacuum distillation, and then filter with a filter membrane with a pore size of 0.6 μm to obtain a clear liquid, which is the fermentation and extract of Cassia seed and wild chrysanthemum.

[0106] 3. Weigh the raw materials: 170g of 0.9% NaCl solution, 13g of sodium hyaluronate, 2.3g of lutein, 0.7g of natural borneol powder (nanoscale), 22g of fermented extract of cassia seed and wild chrysanthemum (the mass ratio of cassia seed: wild chrysanthemum is 1.8:1), 21g of eggshell membrane extract, 8g of triterpenoids from the root of Rosa laevigata (Rosa laevigata sapogenin, for the preparation method, see 201810455214.6).

[0107] 4. Add sodium hyaluronate, lutein, natural borneol powder, fermentation and extract of cassia seed and wild chrysanthemum, eggshell membrane extract, and triterpenoids from the root of Rosa laevigata to 0.9% NaCl solution, stir evenly at 2600 r / min, and sterilize to obtain an eye protection solution based on natural extracts.

[0108] Comparative Example 2: The difference from Example 5 is that the fermentation and extract of Cassia seed and wild chrysanthemum is replaced by the Cassia seed and wild chrysanthemum water extract obtained by conventional water extraction method.

[0109] A method for preparing an eye protection solution based on natural extracts comprises the following steps:

[0110] 1. Preparation of eggshell membrane extract:

[0111] A. Collect eggshell membranes, crush them, add 3 times the volume of 0.6% saline, and soak for 19 minutes;

[0112] B. Take out the eggshell membrane powder and immerse it in acetic anhydride solution (1 mol / L) for 4.5 min; then immerse it in gelatinized starch solution (prepared by mixing gelatinized starch with 7 times volume of water) containing coupling agent (terminated amino silane coupling agent), and irradiate it at the same time, with an irradiation dose of 0.41 kGy and an irradiation time of 13 min;

[0113] C. Filter out the eggshell membrane powder, wash it twice with water, first hydrolyze it with pepsin (pH 2, 37°C, 5h), then hydrolyze it with alkaline protease (pH 9, 53°C, 1.5h), and add 7.5U / mg of enzyme;

[0114] D. Ultrafiltration to obtain ultrafiltration separation components with a molecular weight of 5~10kDa.

[0115] 2. Weigh the raw materials: 170 g of 0.9% NaCl solution, 13 g of sodium hyaluronate, 2.3 g of lutein, 0.7 g of natural borneol powder (nanoscale), 22 g of cassia seed and wild chrysanthemum water extract (the mass ratio of cassia seed: wild chrysanthemum is 1.8:1), 21 g of eggshell membrane extract, 8 g of Rosa laevigata root triterpenoids (Rosa laevigata sapogenin, preparation method see 201810455214.6).

[0116] 3. Add sodium hyaluronate, lutein, natural borneol powder, cassia seed and wild chrysanthemum water extract, eggshell membrane extract, and Rosa laevigata root triterpenoids into 0.9% NaCl solution, stir evenly at 2600r / min, and sterilize to obtain eye protection solution based on natural extracts.

[0117] How to use: You can pack the eye protection solution in a spray bottle and spray it into your eyes 2 to 3 times, 3 to 4 times a day.

[0118] Test Example 1 Experiment on the effect of Examples 1 to 5 and Comparative Examples 1 to 2 on tear secretion in dry eye model rats

[0119] Grouping: D1, 40 rats with qualified corneal fluorescence staining scores and tear secretion were selected and divided into 8 groups (model control group, experimental groups 1-5, and comparison groups 1-2), with 5 rats in each group and no significant statistical difference.

[0120] Modeling: D2, 20 μL of hypertonic solution was dripped into the conjunctival sac of both eyes of the animal, 5 times / day, with an interval of about 2 hours between each drip, for 27 consecutive days. After dripping, the eyelids of the animal were passively closed for about 90 seconds to establish the model.

[0121] Administration: The model control group did not receive additional administration, and the experimental groups 1 to 5 were respectively administered with the eye protection solution prepared in Examples 1 to 5 (sprayed 24 times a day, approximately equivalent to 6 times the clinical dosage), and the comparative groups 1 to 2 were respectively administered with the eye protection solution prepared in Comparative Examples 1 to 2 (sprayed 24 times a day), and used continuously for 20 days.

[0122] Test: The tear secretion volume (mm) of both eyes of rats in each group was measured on D10 and D20, and the mean was calculated. The results are shown in Table 1.

[0123] Table 1 The tear secretion results of each group of rats

[0124] project D0 D10 D20 Model control group 10.22 7.04 7.01 Experimental Group 1 10.07 7.93 10.05 Experimental Group 2 10.19 8.13 10.20 Experimental Group 3 10.25 7.86 10.07 Experimental Group 4 9.97 8.16 10.11 Experimental Group 5 10.30 7.89 10.28 Comparison group 1 10.01 7.08 8.26 Comparison of 2 groups 10.14 7.39 9.14

[0125] It can be seen from Table 1 that after 20 days of continuous administration, the tear secretion of rats basically returned to normal, and the dry eye syndrome of rats can be effectively treated.

[0126] The eye protection liquid of the present invention is prepared by using sodium hyaluronate, lutein, natural borneol powder, cassia seed and wild chrysanthemum water extract, eggshell membrane extract, and Rosa laevigata root triterpenoids. The raw material types are relatively small, the liquid contains a variety of natural extracts, is alcohol-free, and has low irritation. The eye protection liquid is suitable for people with sensitive eyes, has a simple method of use, and can effectively improve dry eye syndrome.

[0127] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. Such modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. An eye protection solution based on natural extracts, characterized in that: The method comprises the following raw materials in parts by weight: 100-200 parts of 0.9% NaCl solution, 12-14 parts of sodium hyaluronate, 1-3 parts of lutein, 0.1-1 parts of natural borneol powder, 16-25 parts of fermented extracts of cassia seeds and wild chrysanthemum, 17-22 parts of eggshell membrane extract, and 6-10 parts of triterpenes from the root of Rosa laevigata; The method for preparing the eggshell membrane extract comprises the following steps: A. Collect eggshell membranes, crush them, add 2-3 times the volume of 0.6% saline, and soak for 15-20 minutes; B. Take out the eggshell membrane powder and immerse it in acetic anhydride solution for 4-6 minutes; then immerse it in gelatinized starch solution containing coupling agent, and irradiate it at the same time, the irradiation dose is 0.35-0.42 kGy, and the irradiation time is 10-15 minutes; C. Filter out the eggshell membrane powder, wash it with water 1-3 times, first hydrolyze it with pepsin, then with alkaline protease, and add 7-8U / mg of enzyme; D. Ultrafiltration to obtain ultrafiltration separation components with a molecular weight of 5-10 kDa; The preparation method of the fermentation and extract of Cassia seed and wild chrysanthemum is as follows: A. Wash Cassia seed and wild chrysanthemum, dry them in the sun, freeze them at a low temperature below -25°C and grind them into mixed plant powder; B. Add water and yeast to the plant mixed powder, mix well, ferment at 25-30℃ for 4-6h, then steam at 100-105℃ for 5-8min, take out and cool to 35-38℃ while stirring, then add lactic acid bacteria, mix well, ferment at 35-38℃ for 1-2h, then steam at 95-100℃ for 1-3min; C. Ultrasonic assisted extraction after centrifugation, with an ultrasonic power of 300-400 W, an ultrasonic frequency of 30-40 kHz, and a time of 25-30 min, followed by microfiltration, with a filter membrane pore size of 0.6-0.8 μm to obtain a filtrate; D. Adjust the pH of the filtrate to 7-7.2, extract with an equal volume of ethanol, recover the ethanol by vacuum distillation, and then filter with a filter membrane with a pore size of 0.5-0.7 μm to obtain a clear liquid, which is the fermentation and extract of Cassia seed and wild chrysanthemum.

2. The eye protection solution based on natural extracts according to claim 1, characterized in that: The eye protection solution is packaged in a spray bottle.

3. The eye protection solution based on natural extracts according to claim 2, characterized in that: The mass ratio of cassia seed to wild chrysanthemum in the fermentation and extract of cassia seed and wild chrysanthemum is 1.5-2:

1.

4. The eye protection solution based on natural extracts according to claim 3, characterized in that: The particle size of the natural borneol powder is nanometer level.

5. The eye protection solution based on natural extracts according to claim 4, characterized in that The concentration of the acetic anhydride solution is 1-1.3 mol / L.

6. The eye protection solution based on natural extracts according to claim 5, characterized in that: The coupling agent is an amino-terminated silane coupling agent.

7. The eye protection solution based on natural extracts according to claim 6, characterized in that: The gelatinized starch solution is prepared by mixing gelatinized starch with 4 to 8 times the volume of water.

8. The eye protection solution based on natural extracts according to claim 7, characterized in that: in, The triterpene compound of the root of Rosa laevigata is Rosa laevigata sapogenin.

9. A method for preparing an eye protection solution based on natural extracts according to claim 8, characterized in that: The steps are: add sodium hyaluronate, lutein, natural borneol powder, fermentation and extract of cassia seed and wild chrysanthemum, eggshell membrane extract, and triterpenoids of Rosa laevigata root into 0.9% NaCl solution, stir evenly at 2000-3000 r / min, and obtain eye protection solution based on natural extracts after sterilization.