Bacteria-enzyme synergistic fermented feed containing terrestrial-source pennisetum sinese and preparation method and application of bacteria-enzyme synergistic fermented feed

Abstract

The invention relates to the technical field of fermented feeds, in particular to a bacterium-enzyme synergistic fermented feed containing terrestrial-source pennisetum sinese and a preparation method and application of the bacterium-enzyme synergistic fermented feed. The preparation method of the bacterium-enzyme synergistic fermented feed containing the ground-source pennisetum sinese comprises the following steps: S1, carrying out superfine grinding and rubbing on pennisetum sinese sections to obtain micronized pennisetum sinese; s2, bacillus subtilis, lactobacillus plantarum, cellulase, xylanase and water are mixed and fermented, and a zymophyte aqueous solution is obtained; s3, mixing the zymophyte aqueous solution and the micronized pennisetum sinese, and performing solid-state fermentation to obtain the bacterium-enzyme synergistic fermented feed. According to the application, high-molecular carbohydrates in the pennisetum sinese are converted into low-molecular carbohydrates through a bacterium-enzyme synergistic fermentation technology, the content of nutritional ingredients such as protein is increased, the use of raw materials is expanded from the perspective of fiber nutrition, the health of pig herds is improved, and the nutritional value of the feed is improved.

Description

Technical Field

[0001] The invention relates to the technical field of fermented feed, and in particular to a fungus-enzyme-cooperatively fermented feed containing endogenous giant fungus grass, and a preparation method and application thereof. Background Art

[0002] Giant Juncao is a perennial upright clump-type plant of the genus Pennisetum in the Poaceae family. As a high-quality endogenous forage grass, Giant Juncao is widely distributed throughout my country and has the characteristics of rapid growth, high yield, and strong stress resistance. Endogenous feed uses a large amount of endogenous local green crop byproducts to replace concentrated feed, which can effectively save feed grain.

[0003] However, the cellulose and lignin content in giant fungus grass is high. These high-molecular carbohydrates are difficult to directly digest and absorb in livestock and poultry, limiting its utilization as feed. Traditional feed processing methods are difficult to effectively convert these components, resulting in feed waste and low breeding efficiency. Therefore, it is of great significance to develop a preparation method that can effectively utilize the ground-based giant fungus grass resources and improve its feed value. Summary of the invention

[0004] In view of the above-mentioned shortcomings of the prior art, the present invention provides a fungus-enzyme synergistic fermentation feed containing endogenous giant fungus grass and a preparation method and application thereof, so as to improve the feed value of giant fungus grass.

[0005] To achieve the above object, the scheme of the present invention is as follows:

[0006] In a first aspect, the present invention provides a method for preparing a feed containing endogenous giant fungus grass by enzyme-cooperative fermentation, comprising the following steps:

[0007] S1. Ultrafinely crush and knead the giant fungus grass segments to obtain micronized giant fungus grass;

[0008] S2. Mixing Bacillus subtilis, Lactobacillus plantarum, cellulase, xylanase and water, and fermenting to obtain a fermentation agent aqueous solution;

[0009] S3. Mix the fermentation agent aqueous solution and the micronized giant fungus grass, and perform solid-state fermentation to obtain the bacterial enzyme synergistic fermentation feed.

[0010] Optionally, in step S1, the length of the giant fungus grass segment is 2-4 cm.

[0011] Optionally, in step S2, the mass ratio of Bacillus subtilis, Lactobacillus plantarum, cellulase, xylanase and water is 0.8-1.5:0.8-1.5:0.8-1.5:0.8-1.5:800-2000.

[0012] Optionally, in step S2, the fermentation temperature is 35-40°C, and the fermentation time is 1-3 days.

[0013] Optionally, in step S3, the mass ratio of the fermentation agent aqueous solution to the micronized giant fungus grass is 0.1-0.5:100.

[0014] Optionally, in step S3, the temperature of the solid-state fermentation is 35-40° C., and the duration of the solid-state fermentation is 4-6 days.

[0015] In a second aspect, the present invention provides a fungus-enzyme co-fermented feed containing endogenous giant fungus grass prepared according to the method described above.

[0016] In a third aspect, the present invention provides a livestock and poultry feed, including a fungus-enzyme co-fermented feed containing endogenous giant fungus grass prepared according to the method described above.

[0017] In a third aspect, the present invention provides a method for feeding livestock and poultry, wherein livestock and poultry are fed with livestock and poultry feed comprising the fungus enzyme-cooperated fermented feed containing endogenous giant fungus grass prepared according to the method described above.

[0018] Beneficial effects of the present invention:

[0019] The present invention converts high-molecular carbohydrates in giant grass into low-molecular carbohydrates through the bacterial-enzyme synergistic fermentation technology, and increases the content of nutrients such as protein, thereby expanding the use of raw materials from the perspective of fiber nutrition, improving the health of pigs, and increasing the nutritional value of feed.

[0020] In the present invention, the sour and fragrant smell and enzymolysis produced during the fermentation process improve the palatability of the feed and increase the feed intake of livestock and poultry.

[0021] The invention utilizes the ground-source giant fungus grass as the main raw material, thereby reducing feed costs and improving economic benefits.

[0022] In the present invention, the supporting bacteria-enzyme synergistic integration and liquid feeding technology are used to expand the scope and mode of application of some ground-based feeds that were previously considered worthless or low-value. At the same time, the original deep treatment and discharge mode of manure and wastewater is changed to provide safe return of manure to the fields for the planting industry, realize the planting and breeding cycle, and promote the sustainable development of agriculture and breeding. DETAILED DESCRIPTION

[0023] The present invention is further described below through specific examples, but it should be pointed out that the specific material ratios, process conditions and results described in the embodiments of the present invention are only used to illustrate the present invention and cannot be used to limit the protection scope of the present invention. All equivalent changes or modifications made according to the spirit of the present invention should be included in the protection scope of the present invention. It should be noted that in the present invention, unless otherwise specified, "wt%" refers to mass percentage.

[0024] In the following description, numerous details are discussed to provide a more thorough explanation of embodiments of the present invention; however, it is apparent to one skilled in the art that embodiments of the present invention may be practiced without these specific details.

[0025] In the present invention, the moisture content is detected according to "GB / T6435-2014 Determination of moisture in feed";

[0026] pH was tested using a pH meter;

[0027] The lactic acid content is tested in accordance with "T / NAIA0002-2020 Determination of lactic acid content in fermented feed by high performance liquid chromatography";

[0028] The dry matter degradation rate was tested using the nylon bag method;

[0029] The crude protein content is tested in accordance with GB / T 6432-2018 Determination of crude protein in feeds - Kjeldahl method;

[0030] The acid-soluble protein content is tested in accordance with "NY_T 3801-2020 Determination of Acid-soluble Protein in Feed Raw Materials";

[0031] The content of medium detergent fiber (i.e. neutral detergent fiber) is tested by hot acid method;

[0032] The acid-washed fiber content is tested in accordance with "NY-T 1459-2007 Determination of Acid Washing Fiber in Feed";

[0033] The crude fiber content is tested in accordance with GB / T 6434-2022 Determination of crude fiber content in feed;

[0034] The crude fat content is tested in accordance with GB / T 6433-2006 Determination of crude fat in feed;

[0035] The crude ash content is tested in accordance with GB / T 6438-1992 Determination of crude ash in feed;

[0036] The Ca content is tested in accordance with GB / T 6436-2002 Determination of Calcium in Feed;

[0037] The P content is tested in accordance with GB / T 6437-2018 Spectrophotometric method for determination of total phosphorus in feed.

[0038] (I) Bacterial enzyme screening

[0039] After washing and removing impurities from the fresh giant grass, the grass was cut into small sections of 3 cm in length, and then 5 treatment groups were set up, as shown in Table 1. Except for the fresh giant grass, the other groups used different bacterial enzyme composite products, using a 5 kg breathing bag and fermented at 37 ° C for 5 days. After fermentation, the products were tested for moisture, pH, lactic acid content, dry matter degradation rate, crude protein content, acid-soluble protein content, acid-soluble protein / crude protein, neutral detergent fiber (i.e., neutral detergent fiber) content, acid-washed fiber content, crude fiber content and other indicators. The results are shown in Table 2.

[0040] Table 1 Giant fungus grass fermentation test design

[0041]

[0042] Note: In this table, spore No. 1 refers to Bacillus subtilis, lactic acid No. 2 refers to Lactobacillus plantarum, enzyme refers to a complex composed of cellulase and xylan in a mass ratio of 1:1, and bacterial enzyme refers to a complex composed of Bacillus subtilis, Lactobacillus plantarum, cellulase and xylan in a mass ratio of 1:1:1:1.

[0043] Table 2 Test results of different silage schemes on the fermentation effect of giant fungus grass

[0044]

[0045] As shown in Table 2, fermentation of giant grass with different silage schemes can reduce the pH to about 3.6, increase the lactic acid content by 3.3%-3.8%, the acid-soluble protein content by 0.5%-1.3%, and the acid-soluble protein ratio by 6.7%-30.2%, with little effect on the fiber content, among which bacterial enzyme fermentation 1 has a higher improvement effect; different silage schemes increase the dry matter degradation rate by 0.5-4.0%.

[0046] (II) Exploration of physical treatment methods and conditions

[0047] Fresh giant grass was washed and impurities were removed, then cut into small segments of 3 cm in length, and then two treatment groups were set up, as shown in Table 3. In the fermented giant grass group, the giant grass segments were ultrafinely crushed and shredded, and passed through a 10-mesh sieve. Both groups used 5 kg breathing bags and fermented at 37°C for 2 days. After fermentation, the products were tested for moisture, pH, lactic acid content, dry matter degradation rate, crude protein content, acid-soluble protein content, acid-soluble ratio, medium-wash fiber (i.e., neutral detergent fiber) content, acid-washed fiber content, crude fiber content and other indicators. The results are shown in Table 4.

[0048] Table 3 Experimental design of physical treatment of giant fungus grass

[0049]

[0050] Note: In this table, fresh grass refers to fresh giant fungus grass segments, No. 1 spore refers to Bacillus subtilis, No. 2 lactic acid refers to Lactobacillus plantarum, and enzyme refers to a complex composed of cellulase and xylan in a mass ratio of 1:1.

[0051] Table 4 Test results of physical treatment on the fermentation effect of giant fungus grass

[0052]

[0053] As shown in Table 4, the pH of fermented giant grass finally dropped to 3.74, the lactic acid content increased by 1.8%, the dry matter degradation rate increased to 42.4%, and the acid-soluble protein increased to 15.3%.

[0054] Fresh giant grass was washed and impurities were removed, then cut into small segments of 3 cm in length, and then two treatment groups were set up, as shown in Table 5. In the fermented giant grass group, the giant grass segments were ultrafinely crushed and shredded, and passed through a 10-mesh sieve. Both groups used a 6 kg oil drum with a lid for airtight ventilation, and fermented at 37°C for 2 days. After fermentation, the product was tested for moisture, pH, lactic acid content, dry matter degradation rate, crude protein content, acid-soluble protein content, acid-soluble ratio, medium-wash fiber (i.e., neutral detergent fiber) content, acid-washed fiber content, crude fiber content and other indicators. The results are shown in Table 6.

[0055] Table 5 Experimental design of physical treatment of giant fungus grass

[0056]

[0057] Note: In this table, fresh grass refers to fresh giant fungus grass segments, No. 1 spore refers to Bacillus subtilis, No. 2 lactic acid refers to Lactobacillus plantarum, and enzyme refers to a complex composed of cellulase and xylan in a mass ratio of 1:1.

[0058] Table 6 Evaluation results of physical treatment methods on the fermentation effect of giant fungus grass

[0059]

[0060] As shown in Table 6, the pH of fermented giant grass finally dropped to 3.63, the lactic acid content increased by 2.8%, the dry matter degradation rate increased to 30.8%, and the acid-soluble protein increased to 9.1%.

[0061] From Tables 3 to 6, we can see that the bag swelling is reduced after adding water for fermentation, which may be because the water isolates the air and reduces the contamination of bacteria. This result shows that the effect of adding water for fermentation is significantly higher than that of silage. The fermentation process requires complete exhaustion of gas or continuous shaking to avoid the attachment of bacteria, and there is no obvious change in viscosity after fermentation.

[0062] (III) Evaluation of the chemical treatment scheme on the fermentation effect of giant fungus grass

[0063] After washing and removing impurities from fresh giant grass, the grass was cut into 3 cm long segments, and then several treatment groups were set, as shown in Table 7, 0-5 were all fresh grass tests, and fermentation was carried out according to the conditions shown in Table 7. After fermentation, the product was tested for moisture (H2O), crude protein content (CP), crude fat content (EE), crude ash content (Ash), Ca content, P content, crude fiber content (CF), mid-wash fiber content (DNF), acid washed fiber content (ADF) and other indicators, and the results are shown in Table 8;

[0064] After fermentation, the product pH, glucose content, xylose content, total sugar content, lactic acid content, total acid content, and total acid increment were tested. The results are shown in Table 9.

[0065] The dry matter digestibility, ash content, acid detergent fiber content, neutral detergent fiber content, actual increase in dry matter digestibility due to bacterial enzyme action, and actual decrease in crude fiber due to bacterial enzyme action of the product were tested. The results are shown in Table 10.

[0066] Table 7 Experimental design for evaluating the effect of chemical treatment on the fermentation of giant fungus grass

[0067]

[0068] Note: In this table, alkali treatment refers to the process of crushing giant grass segments and mixing them evenly with 1 wt% sodium hydroxide solution (W / V) at a solid-liquid ratio of 2:3. Bacterial enzyme synergistic fermentation refers to the same treatment method of adding bacterial enzymes as in (I).

[0069] Table 8 Test results of the effect of chemical treatment on the fermentation effect of giant fungus grass

[0070]

[0071] As shown in Table 8, after fermentation, the ash content in giant fungus grass increased by 10%-12%, and the crude fiber decreased by 14%-17%, but the mixed and dried samples of treatment group 0 also achieved similar results.

[0072] Table 9 Effect of chemical treatment scheme on the fermentation effect of giant fungus grass

[0073]

[0074] As shown in Table 9, after fermentation, the pH of the room temperature reaction samples dropped from 5.31 to pH 3.8-4.0 (groups 7 and 9), and the pH of the samples of the insulation reaction dropped from 5.31 to 3.4-3.6 (groups 1-5, 8 and 10), which is lower than the expected pH 3.7-3.8. This result shows that after the fermentation is completed, the viscosity is significantly reduced. If the temperature is lowered on the first day, it is obvious that the viscosity of the fermented giant grass is higher, mainly because cellulase plays a greater role in the degradation of giant grass.

[0075] Table 10 Evaluation of the chemical treatment scheme on the fermentation effect of giant fungus grass

[0076]

[0077] As shown in Table 10, according to the calculation of the previous test, for every 1% increase in ash content, the dry matter digestibility increases by 1.13%. Therefore, the ash content of sample 0 increases by 10.91%, and the dry matter digestibility actually increases by 12.2% due to ash content, and the actual increase is 30.3%. The digestibility of sample 0 increased by 5.94% due to drying and enzymatic hydrolysis. The reason may be that the enzyme and grass undergo enzymatic hydrolysis during the drying and heating process. The actual increase in dry matter digestibility is about 7.28%-11.35%.

[0078] (IV) Effects of fermented giant fungus grass on pig production performance

[0079] 181-day-old growing pigs with similar body weight were selected and randomly divided into 2 groups, namely a control group and a test group (as shown in Table 11), with 12 replicates in each group, and the initial body weight was measured;

[0080] Each group of pigs was fed a diet, wherein the control group was fed a basal diet, which was a corn-soybean meal diet, and the experimental group was fed with an additional fermented giant fungus grass (8wt% of the mass of the basal diet) on the basis of the basal diet (the specific preparation steps were the same as the enzyme fermentation 3 in Table 1, except that water of the same mass as fresh giant fungus grass segments was added). The energy and amino acid levels of each treatment group were consistent, and the experimental period was 316 stages, a total of 25 days. During this period, the pigs had free access to food, sufficient drinking water, and were immunized according to the normal immunization procedure. The average weight at the end (i.e. at the end) was tested, the feed-to-meat ratio (excluding giant fungus grass), feed-to-meat ratio (containing wet giant fungus grass), feed-to-meat ratio (containing dry giant fungus grass), the authenticity rate was tested, the average daily weight gain was calculated, and the average daily feed intake was statistically analyzed. The results are shown in Table 12.

[0081] Table 11 Experimental design of fermented giant fungus grass on the production performance of pigs at stages 3-16

[0082] Group Feeding plan Control group Corn-soybean meal based diet Experimental Group Corn-soybean meal based diet + 8wt% fermented giant fungus grass

[0083] Table 12 Effect of fermented giant fungus grass on production performance of pigs at stage 316

[0084]

[0085] It can be seen from Table 12 that compared with the control group, the experimental group reduced the daily feed intake, average daily weight gain and feed-to-meat ratio of pigs. After conversion to dry matter, there was no significant effect on the feed-to-meat ratio, which may be helpful to improve the authenticity rate.

[0086] 103-day-old growing pigs with similar body weight were selected and randomly divided into 4 groups, namely control group, experimental group 1, experimental group 2 and experimental group 3 (as shown in Table 13), with 4 replicates in each group and 12 pigs in each replicate, and the initial body weight was measured;

[0087] The pigs in each group were fed with diets, wherein the control group was fed a basal diet, which was a corn-soybean meal diet; the experimental group 1 was fed with an additional 8wt% of the basal diet mass of fermented giant fungus grass (the specific preparation steps were the same as the bacterial enzyme fermentation 3 in Table 1, except that water of the same mass as fresh giant fungus grass segments was added), 0.1% of the basal diet mass of Lactobacillus plantarum and 100% of the basal diet mass of water; the experimental group 2 was fed with an additional 24wt% of the basal diet mass of fermented giant fungus grass (the specific preparation steps were the same as the bacterial enzyme fermentation 3 in Table 1, except that water of the same mass as fresh giant fungus grass segments was added), and the fermented giant fungus grass (the specific preparation steps were the same as the bacterial enzyme fermentation 3 in Table 1, except that water of the same mass as fresh giant fungus grass segments was added). In addition to water with the same mass of fresh giant grass segments, it is the same as the bacterial enzyme fermentation 3 in Table 1) and water with 80% of the mass of the basic diet. In the experimental group 3, 20wt% of the mass of the basic diet of fermented giant grass (the specific preparation steps are the same as the bacterial enzyme fermentation 3 in Table 1 except for the addition of water with the same mass of fresh giant grass segments) and water with 75% of the mass of the basic diet are added on the basis of the basic diet. The energy and amino acid levels of each treatment group are consistent. The experimental period is 313 stages, a total of 25 days. During this period, the pigs have free access to food, sufficient drinking water, and are immunized according to the normal immunization procedure.

[0088] The average weight at the beginning and end of the test, the feed-to-meat ratio, the authenticity rate, the average daily weight gain, the average daily feed intake and the diarrhea rate were calculated. The results are shown in Table 14.

[0089] The feed-to-meat ratio (including wet grass), average daily feed intake (including wet grass), feed-to-meat ratio (converted to hay) and average daily feed intake (converted to hay) were calculated. The results are shown in Table 15.

[0090] At the end of the experiment, the serum albumin / globulin ratio, albumin (g / L) content, alanine aminotransferase (U / L) content, triglyceride (nmol / L) and other biochemical indices of each group were tested, and the results are shown in Table 16;

[0091] The feed cost was calculated and the results are shown in Table 17.

[0092] Table 13 Experimental design of the effect of fermentation of giant fungus grass on the production performance of pigs at stage 313 by bacteria-enzyme synergistic process

[0093]

[0094]

[0095] Table 14 Test results of the effect of fermentation of giant fungus grass by bacterial enzyme synergistic process on the production performance of pigs at stage 313

[0096]

[0097] As shown in Table 14, compared with the control group, the feed-to-meat ratio of the test groups 1-3 was reduced, the average daily feed intake was significantly reduced, and the daily weight gain was significantly increased. This result shows that in the present invention, fermented giant fungus grass can improve the feed-to-meat ratio, thereby improving the edible performance of the feed.

[0098] Table 15 Test results of the effect of fermentation of giant fungus grass by bacterial enzyme synergistic process on the production performance of pigs at stage 313

[0099] deal with Feed to meat ratio (including wet grass) Average daily feed intake (including wet grass, g) Feed to meat ratio (half equivalent) Average daily feed intake (in terms of hay, g) Control group <![CDATA[2.30±0.07 b ]]> 2399±64 2.30±0.07 2399±64 Experimental Group 1 <![CDATA[2.43±0.05 a ]]> 2554±31 2.31±0.05 2424±31 Experimental Group 2 <![CDATA[2.44±0.08 a ]]> 2547±46 2.31±0.08 2418±46 Experimental Group 3 <![CDATA[2.43±0.04 a ]]> 2542±77 2.31±0.04 2413±77

[0100] It can be seen from Table 15 that compared with the control group, the average daily feed intake of experimental groups 1-3 increased significantly. The reason may be that the energy value of giant fungus grass is low, which reduces the energy value of complete feed after conversion.

[0101] Table 16 Effects of fermentation of giant fungus grass by bacterial enzyme synergistic process on serum biochemical parameters of 313 pigs

[0102] project Control group Experimental Group 1 Experimental Group 2 Experimental Group 3 Albumin / globulin ratio 1.54±0.20 1.59±0.22 1.62±0.24 1.66±0.30 Albumin content (g / L) 35.92±2.91 36.02±2.56 37.52±1.53 36.80±3.14 Alanine aminotransferase content (U / L) 48.25±9.83 47.12±7.62 66.12±9.94 63.37±20.03 Triglyceride content (nmol / L) 0.41±0.17 0.38±0.08 0.38±0.11 0.39±0.11 Alkaline phosphatase content (U / L) 159.87±23.86 134.37±16.70 151.00±21.74 157.25±19.11 Globulin content (g / L) 23.79±3.04 23.14±3.26 23.46±3.23 22.46±2.57 Lactate dehydrogenase content (U / L) 732.37±201.74 804.50±433.00 896.50±395.83 1623.13±2367.62 Aspartate / alanine aminotransferase content (U / L) 1.03±0.46 1.18±0.73 1.18±1.07 1.71±1.60 Aspartate aminotransferase content (U / L) 50.12±25.76 57.5±45.5 80.75±82.04 131.75±159.80 Total cholesterol content (nmol / L) 2.24±0.24 2.29±0.41 2.41±0.15 2.28±0.27 Total protein content (g / L) 59.71±4.54 59.16±3.91 60.99±2.76 59.26±2.31

[0103] As shown in Table 16, compared with the control group, the transaminase levels in test group 2 and test group 3 were significantly increased. Alanine aminotransferase is an important indicator of the degree of liver disease. The higher the serum transaminase level, the higher the degree of liver disease. It may be that the fermented grass has been used for a long time, producing miscellaneous bacteria, etc. It is recommended that the fermented grass should not be used for more than 10 days.

[0104] Table 17 Effect of the fermentation of giant fungus grass by bacterial enzyme synergistic process on the feed cost of 313 pigs

[0105]

[0106]

[0107] Note: This table is calculated based on feed price of RMB 3,000 / ton and wet grass price of RMB 200 / ton (chemical solution is calculated based on RMB 300 / ton).

[0108] It can be seen from Table 17 that compared with the control group, the feed cost of pigs in the experimental groups 1-3 was reduced by 0.01-0.02 yuan / jin.

[0109] In summary, in the present invention, the fermented feed with bacteria and enzymes slightly reduces the feed-to-meat ratio of pigs and reduces the cost of pigs by 0.01-0.02 yuan / jin, and has no significant negative impact on the feed intake and daily weight gain of complete feed; it has little effect on the feed intake and weight gain of fattening pigs. The fermented feed with bacteria and enzymes can significantly increase the content of alanine aminotransferase, which may be related to the fact that giant fungus grass fermented with water cannot be preserved for a long time.

[0110] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the technology may modify or change the above embodiments without violating the spirit and scope of the present invention. Therefore, all equivalent modifications or changes made by a person of ordinary skill in the art without departing from the spirit and technical ideas disclosed by the present invention shall still be covered by the claims of the present invention.

Claims

1. A method for preparing a feed containing endogenous giant fungus grass and enzyme-cooperative fermentation, characterized in that: The following steps are involved: S1. Ultrafinely crush and knead the giant fungus grass segments to obtain micronized giant fungus grass; S2. Mixing Bacillus subtilis, Lactobacillus plantarum, cellulase, xylanase and water, and fermenting to obtain a fermentation agent aqueous solution; S3. Mix the fermentation agent aqueous solution and the micronized giant fungus grass, and perform solid-state fermentation to obtain the bacterial enzyme synergistic fermentation feed.

2. The method for preparing the fungus enzyme-cooperative fermented feed containing endogenous giant fungus grass according to claim 1, characterized in that: In step S1, the length of the giant fungus grass segment is 2-4 cm.

3. The method for preparing the fungus enzyme-cooperative fermented feed containing endogenous giant fungus grass according to claim 1, characterized in that: In step S2, the mass ratio of the Bacillus subtilis, Lactobacillus plantarum, cellulase, xylanase and water is 0.8-1.5:0.8-1.5:0.8-1.5:0.8-1.5:800-2000.

4. The method for preparing the fungus enzyme-cooperative fermented feed containing endogenous giant fungus grass according to claim 1, characterized in that: In step S2, the fermentation temperature is 35-40°C, and the fermentation time is 1-3 days.

5. The method for preparing the fungus enzyme-cooperative fermentation feed containing endogenous giant fungus grass according to claim 1, characterized in that: In step S3, the mass ratio of the fermentation agent aqueous solution to the micronized giant fungus grass is 0.1-0.5:

100.

6. The method for preparing the fungus enzyme-cooperative fermented feed containing endogenous giant fungus grass according to claim 1, characterized in that: In step S3, the temperature of the solid-state fermentation is 35-40° C., and the duration of the solid-state fermentation is 4-6 days.

7. A fungus-enzyme synergistically fermented feed containing endogenous giant fungus grass prepared according to the method according to any one of claims 1 to 6.

8. A livestock and poultry feed, characterized in that: It comprises a fungus enzyme synergistically fermented feed containing endogenous giant fungus grass prepared according to the method described in any one of claims 1-6.

9. A method for feeding livestock and poultry, characterized in that: Livestock and poultry are fed with livestock and poultry feed comprising the fungus enzyme-cooperated fermented feed containing endogenous giant fungus grass prepared according to the method described in any one of claims 1 to 6.

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