Sample loading treatment device of chromatography system

Abstract

The present invention discloses a chromatography system sample loading treatment device, which comprises a box body, a sample liquid treatment device and a power mechanism, a heating seat is fixedly installed in the box body, the sample liquid treatment device is rotatably installed in the heating seat, an auxiliary stirring mechanism is installed at the bottom of the sample liquid treatment device, and the power mechanism is in transmission connection with the sample liquid treatment device and the auxiliary stirring mechanism. The power mechanism can drive the sample liquid processor and the auxiliary stirring mechanism to rotate synchronously, a liquid inlet is formed in the top of the box body, a liquid outlet is formed in the side wall of the box body, and liquid flows out of the bottom of the sample liquid processor and is connected to the liquid outlet through a pipeline. Under the action, a better defoaming effect can be formed on the sample liquid, the uniformity of the output sample liquid can also be guaranteed, the device also has the advantages of few residues and small loss, and the device is also provided with a heating structure, a temperature control structure, a cleaning structure and the like, so that the requirements of feeding temperature control and later cleaning and maintenance can be met.

Description

Technical Field

[0001] The invention relates to the technical field of pharmaceutical production equipment, and more specifically to a sample loading processing device of a chromatography system. Background Art

[0002] Chromatography systems are widely used in the fields of biology and biopharmaceuticals. They are important application equipment for purification of proteins and peptides, separation and purification of biomolecules, and production and research and development of biopharmaceuticals. The sample liquid used in the chromatography system is a suspension with a large content of polysaccharides and proteins. There are obvious bubbles in the sample liquid. The large number of bubbles in the sample liquid will affect the chromatography operation. Therefore, how to remove the bubbles in the sample liquid before loading is an urgent problem to be solved. During the sample liquid loading process, temperature control and maintaining the uniformity of the sample liquid are also required. These are also difficult problems that need to be solved urgently. The sample liquid used in the chromatography system also has the characteristics of high value. Since there is no special loading equipment for loading, there are still problems such as large sample liquid residue and large loss during the loading operation. The above problems urgently need to be solved by designing a special sample liquid loading and processing equipment, and this case is born out of this. Summary of the invention

[0003] The purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and to provide a sample loading processing device for a chromatography system. The device is designed with a funnel-shaped sample liquid processor, an auxiliary stirring mechanism is installed at the bottom of the sample liquid processor, and a defoaming layer is installed inside the sample liquid processor. The sample liquid processor and the auxiliary stirring mechanism can achieve synchronous rotation in different directions. Under this action, a better defoaming effect can be formed on the sample liquid, and the uniformity of the output sample liquid can also be guaranteed. The device also has the advantages of less residue and less loss.

[0004] In order to achieve the above object, the present invention adopts the following technical solution:

[0005] A sample loading processing device for a chromatography system comprises a housing, a sample liquid processor and a power mechanism, wherein a heating seat is fixedly installed in the housing, the sample liquid processor is rotatably installed in the heating seat, the sample liquid processor is in the shape of a conical funnel, the top of the sample liquid processor is open, the top diameter of the sample liquid processor is larger than the bottom diameter, an auxiliary stirring mechanism is installed at the bottom of the sample liquid processor, the power mechanism is transmission-connected with the sample liquid processor and the auxiliary stirring mechanism, the power mechanism can drive the sample liquid processor and the auxiliary stirring mechanism to rotate synchronously, a liquid inlet is provided at the top of the housing, the liquid inlet is connected with an infusion tube, the infusion tube extends downward to the top open position of the sample liquid processor, a liquid outlet is provided on the side wall of the housing, liquid is discharged from the bottom of the sample liquid processor, and a pipeline is provided at the bottom of the sample liquid processor connected to the liquid outlet.

[0006] Furthermore, the auxiliary stirring mechanism includes a rotating shaft, which is upright and rotatably installed at the bottom of the sample liquid processor, the top of the rotating shaft is inserted into the sample liquid processor, and a stirring blade is installed on the outer wall of the inserted part of the rotating shaft.

[0007] Furthermore, a liquid outlet channel is opened in the rotating shaft, and a plurality of liquid outlet holes are opened on the outer wall of the inserted part of the rotating shaft. The liquid outlet holes are connected to the liquid outlet channel, and the bottom pipeline of the liquid outlet channel is connected to the liquid outlet. A first control valve is installed on the connecting pipeline from the liquid outlet channel to the liquid outlet.

[0008] Furthermore, the power mechanism includes a motor, a first transmission bevel gear and a second transmission bevel gear, an active bevel gear is installed at the output end of the motor, the first transmission bevel gear is fixedly installed at the bottom of the sample liquid processor, the second transmission bevel gear is fixedly installed on the outer wall of the rotating shaft, the first transmission bevel gear and the second transmission bevel gear are symmetrically installed, the first transmission bevel gear and the second transmission bevel gear are meshed with the active bevel gear, and the active bevel gear can drive the first transmission bevel gear and the second transmission bevel gear to rotate synchronously in opposite directions.

[0009] Furthermore, the sample liquid processor is made of heat-conductive material, and the outer wall of the sample liquid processor fits the inner cavity of the heating seat.

[0010] Furthermore, a defoaming layer is fixedly installed in the inner cavity of the sample liquid processor, and the defoaming layer is made of a grid screen, and a gap is left between the defoaming layer and the inner wall of the sample liquid processor.

[0011] Furthermore, the heating seat and the sample liquid processor are installed in the middle section of the box body, and a monitoring area is provided on the top of the box body. The monitoring area is installed with a temperature measuring element and a liquid level monitor. The liquid level monitor is installed with a probe extending downward, and the probe extends into the cavity of the sample liquid processor. A waste liquid area is provided at the bottom of the box body, and the bottom pipeline of the liquid outlet channel is connected to the waste liquid area. A second control valve is installed on the connecting pipeline between the liquid outlet channel and the waste liquid area, and the first control valve and the second control valve are not opened at the same time.

[0012] Furthermore, an annular fixing seat is installed in the box body, and the fixing seat is arranged between the monitoring area and the sample liquid processor. A plurality of spray nozzles are evenly installed along the annular length direction of the fixing seat, and the spray nozzles are arranged to face the inner wall of the sample liquid processor. A water storage area is provided at the bottom of the box body, and the water storage area is connected to the spray nozzle pipeline. An ultraviolet lamp is installed directly above the sample liquid processor.

[0013] The beneficial effects of the present invention are:

[0014] 1. The present invention is designed with a funnel-shaped sample liquid processor, an auxiliary stirring mechanism is installed at the bottom of the sample liquid processor, and a defoaming layer is installed inside the sample liquid processor. The sample liquid processor and the auxiliary stirring mechanism can realize synchronous rotation in different directions. Under this action, a better defoaming effect can be formed on the sample liquid, and the uniformity of the output sample liquid can also be guaranteed.

[0015] 2. Since the sample liquid processor itself can rotate, the sample liquid is unlikely to remain inside the sample liquid processor. The present invention also has the advantages of less residue and less loss.

[0016] 3. The present invention is also designed with heating, temperature control, cleaning and other structures, which can meet the needs of feeding temperature control and later cleaning and maintenance. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic diagram of the appearance structure of a sample loading processing device of a chromatography system in this embodiment;

[0018] Figure 2 Schematic diagram of the internal structure of a sample loading processing device of a chromatography system in this embodiment;

[0019] Figure 3 for Figure 2 A magnified view of the position in the figure;

[0020] Figure 4 It is a schematic diagram of the local structure of the defoaming layer in this embodiment.

[0021] Figure numerals: box body 1, liquid inlet 11, infusion tube 111, monitoring area 12, temperature measuring element 121, liquid level monitor 122, probe 1221, ultraviolet lamp 123, liquid outlet 13, first control valve 131, waste liquid area 14, second control valve 141, fixing seat 15, spray nozzle 151, water storage area 16, heating seat 2, sample liquid processor 3, defoaming layer 31, auxiliary stirring mechanism 4, rotating shaft 41, liquid outlet channel 411, liquid outlet hole 412, stirring blade 42, power mechanism 5, motor 51, driving bevel gear 52, first transmission bevel gear 53, second transmission bevel gear 54. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] like Figure 1-Figure 4The sample loading processing device of a chromatography system shown in the figure is connected to the inlet front end of the chromatography system, and includes a housing 1, a sample liquid processor 3 and a power mechanism 5. A heating seat 2 is fixedly installed in the housing 1, and the sample liquid processor 3 is rotatably installed in the heating seat 2. The sample liquid processor 3 is in the shape of a conical funnel, and the top of the sample liquid processor 3 is open. The top diameter of the sample liquid processor 3 is larger than the bottom diameter. A liquid inlet 11 is provided on the top of the housing 1, and the sample liquid is input from the liquid inlet 11. The liquid inlet 11 is connected to an infusion tube 111, and the infusion tube 111 extends downward to the top open position of the sample liquid processor 3. The input sample liquid is guided by the infusion tube 111 to be contained in the sample liquid processor 3, and then is processed. The heating seat 2 provides the required temperature for the sample liquid, and the sample liquid processor 3 can be relatively The heating seat 2 rotates, and an auxiliary stirring mechanism 4 is installed at the bottom of the sample liquid processor 3. The power mechanism 5 is transmission-connected with the sample liquid processor 3 and the auxiliary stirring mechanism 4. The power mechanism 5 can drive the sample liquid processor 3 and the auxiliary stirring mechanism 4 to rotate synchronously. The rotation of the sample liquid processor 3 can stir the sample liquid and remove bubbles in the sample liquid. The sample liquid processor 3 discharges liquid at the bottom. The auxiliary stirring mechanism 4 is also installed at the bottom of the sample liquid processor 3, which can form a stirring effect on the output sample liquid to ensure its uniformity. A liquid outlet 13 is provided on the side wall of the box body 1, and a pipeline is connected to the liquid outlet 13 at the bottom of the sample liquid processor 3. The liquid outlet 13 is the final liquid outlet position of the present invention. The pipeline of the liquid outlet 13 is connected to the inlet of the chromatography system. The sample liquid output by the present invention has the advantages of few bubbles and good uniformity.

[0024] like Figure 2 and Figure 3 As shown, the auxiliary stirring mechanism 4 includes a rotating shaft 41, which is upright and rotatably installed at the bottom of the sample liquid processor 3. The top of the rotating shaft 41 is inserted into the sample liquid processor 3, and a stirring blade 42 is installed on the outer wall of the inserted part of the rotating shaft 41. When the rotating shaft 41 rotates, the stirring blade 42 can form a stirring effect on the sample liquid at the bottom of the sample liquid processor 3. The stirring effect directly acts on the sample liquid output position, which can ensure the uniformity of the output sample liquid.

[0025] like Figure 3 As shown, a liquid outlet channel 411 is provided in the rotating shaft 41, and a plurality of liquid outlet holes 412 are provided on the outer wall of the inserted part of the rotating shaft 41, and the liquid outlet holes 412 are connected to the liquid outlet channel 411. The present invention cleverly uses the rotating shaft 41 as an output pipeline, and the rotating shaft 41 can be used as a stirring mechanism and can meet the needs of outputting sample liquid. A rotary joint is installed at the bottom of the liquid outlet channel 411 (i.e., the bottom of the rotating shaft 41), and then the pipeline is connected to the liquid outlet 13 to meet the output of the sample liquid. A first control valve 131 is installed on the connecting pipeline from the liquid outlet channel 411 to the liquid outlet 13, and the first control valve 131 can control the opening and closing of the connecting pipeline from the liquid outlet channel 411 to the liquid outlet 13, as shown in FIG. Figure 2As shown, the heating seat 2 and the sample liquid processor 3 are installed in the middle section of the box body 1, and a waste liquid area 14 is provided at the bottom of the box body 1. The bottom of the liquid outlet channel 411 is also connected to the waste liquid area 14 by a pipeline. A second control valve 141 is installed on the connecting pipeline between the liquid outlet channel 411 and the waste liquid area 14. The second control valve 141 can control the opening and closing of the connecting pipeline between the liquid outlet channel 411 and the waste liquid area 14. The first control valve 131 and the second control valve 141 are not opened at the same time, that is, the two liquid outlets cannot be opened at the same time. In actual use, they are either closed at the same time or opened alternately. When the liquid is discharged, the first control valve 131 is generally opened first, and the sample liquid is output from the liquid outlet 13. As the sample liquid gradually flows out to only a small amount at the bottom, the first control valve 131 is closed, and the second control valve 141 is opened to allow the residual liquid at the bottom to flow to the waste liquid area 14 (the final residual liquid at the bottom generally has a large bubble content, so it is discarded).

[0026] like Figure 2 As shown, the power mechanism 5 includes a motor 51, a first transmission bevel gear 53 and a second transmission bevel gear 54. The motor 51 is fixedly installed in the box body 1, and an active bevel gear 52 is installed at the output end of the motor 51. The first transmission bevel gear 53 is fixedly installed at the bottom of the sample liquid processor 3, and the second transmission bevel gear 54 is fixedly installed on the outer wall of the rotating shaft 41 (a bracket of the rotating shaft 41 is installed in the box body 1, and the rotating shaft 41 is also rotatably installed on the bracket). The first transmission bevel gear 53 and the second transmission bevel gear 54 are symmetrically installed. The first transmission bevel gear 53 and the second transmission bevel gear 54 are meshed with the active bevel gear 52, and the active bevel gear 52 can drive the first transmission bevel gear 53 to rotate. The wheel 53 and the second transmission bevel gear 54 rotate synchronously in opposite directions. Since the rotation directions of the first transmission bevel gear 53 and the second transmission bevel gear 54 are opposite, the sample liquid processor 3 and the auxiliary stirring mechanism 4 can form a synchronous opposite direction movement effect. Such a reverse movement effect is beneficial to the stirring of the sample liquid, enhances the bubble removal effect and enhances the uniformity guarantee effect. The sample liquid processor 3 is made of heat-conducting material, and the outer wall of the sample liquid processor 3 fits the inner cavity of the heating seat 2. The heating seat 2 can be heated by electricity or heat-conducting oil. The heat of the heating seat 2 can be transferred to the sample liquid processor 3 to form a heating effect on the sample liquid inside to meet the temperature requirement of the loading sample liquid.

[0027] The bubbles in the sample liquid can be effectively removed by the rotation and stirring of the sample liquid processor 3, but the removal effect can be further improved. Figure 2 and Figure 3 As shown, the present invention also has a defoaming layer 31 fixedly installed in the inner cavity of the sample liquid processor 3, as shown in FIG. Figure 4As shown, the defoaming layer 31 is made of a grid screen, and the defoaming layer 31 has mesh holes. A gap is left between the defoaming layer 31 and the inner wall of the sample liquid processor 3. In the process of the sample liquid processor 3 rotating and stirring the sample liquid, the sample liquid will continue to flow inside and outside the defoaming layer 31, and the mesh holes of the defoaming layer 31 can break the bubbles in the sample liquid, which can further improve the defoaming quality. The sample liquid processor 3 and the auxiliary stirring mechanism 4 of the present invention need to rotate at a low and uniform speed to meet the processing requirements. Since the sample liquid processor 3 can rotate, the sample liquid residual on the wall will have a tendency to fall centrifugally, which helps to reduce wall residue and reduce operating losses.

[0028] like Figure 2 As shown, a monitoring area 12 is provided on the top of the box 1, and a temperature measuring element 121 and a liquid level monitor 122 are installed in the monitoring area 12. The temperature measuring element 121 monitors the temperature change in the box 1 in real time to control the liquid temperature of the sample liquid. The liquid level monitor 122 is installed with a probe 1221 extending downward, and the probe 1221 extends into the cavity of the sample liquid processor 3. The liquid level monitor 122 detects the highest liquid level of the sample liquid poured into the sample liquid processor 3 to prevent overflow due to excessive sample liquid being poured in.

[0029] After each shift, the sample liquid processor 3 needs to be cleaned to keep it clean. Under this requirement, Figure 2 As shown, an annular fixing seat 15 is installed in the box body 1, and the fixing seat 15 is arranged between the monitoring area 12 and the sample liquid processor 3. A plurality of spray nozzles 151 are evenly installed along the annular length direction of the fixing seat 15, and the spray nozzles 151 are arranged facing the inner wall of the sample liquid processor 3. A water storage area 16 is provided at the bottom of the box body 1, and the water storage area 16 stores cleaning water. The water storage area 16 is connected to the spray nozzle 151 by a pipeline. During the cleaning operation, the water pump is used to supply water to the spray nozzle 151, and the spray nozzle 151 is sprayed. The wash nozzle 151 sprays water onto the inner wall of the sample liquid processor 3. As the sample liquid processor 3 rotates, the inner wall of the sample liquid processor 3 can be completely rinsed. During the flushing operation, the second control valve 141 is opened, the first control valve 131 is closed, and the cleaning waste water flows to the waste liquid area 14. An ultraviolet lamp 123 is installed directly above the sample liquid processor 3. After flushing, the ultraviolet lamp 123 is turned on for drying and disinfection. The ultraviolet lamp 123 is not turned on during the normal sample liquid processing stage.

[0030] The above is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments. All technical solutions under the concept of the present invention belong to the protection scope of the present invention. It should be pointed out that for ordinary technicians in this technical field, some improvements and modifications without departing from the principle of the present invention should also be regarded as the protection scope of the present invention.

Claims

1. A sample handling device for a chromatography system, characterized in that: The invention comprises a housing (1), a sample liquid processor (3) and a power mechanism (5); a heating seat (2) is fixedly installed in the housing (1); the sample liquid processor (3) is rotatably installed in the heating seat (2); the sample liquid processor (3) is in the shape of a conical funnel; the top of the sample liquid processor (3) is open; the top diameter of the sample liquid processor (3) is larger than the bottom diameter; an auxiliary stirring mechanism (4) is installed at the bottom of the sample liquid processor (3); the power mechanism (5) is connected to the sample liquid processor (3) and the auxiliary stirring mechanism ( 4) a transmission connection, the power mechanism (5) can drive the sample liquid processor (3) and the auxiliary stirring mechanism (4) to rotate synchronously, the top of the box body (1) is provided with a liquid inlet (11), the liquid inlet (11) is connected to a liquid infusion tube (111), the liquid infusion tube (111) extends downward to the top open position of the sample liquid processor (3), the side wall of the box body (1) is provided with a liquid outlet (13), the sample liquid processor (3) discharges liquid at the bottom, and the bottom of the sample liquid processor (3) is provided with a pipeline connected to the liquid outlet (13).

2. The sample loading processing device of a chromatography system according to claim 1, characterized in that: The auxiliary stirring mechanism (4) comprises a rotating shaft (41), which is vertically and rotatably mounted on the bottom of the sample liquid processor (3), and the top of the rotating shaft (41) is inserted into the sample liquid processor (3), and a stirring blade (42) is installed on the outer wall of the inserted part of the rotating shaft (41).

3. The sample loading processing device of a chromatography system according to claim 2, characterized in that: A liquid outlet channel (411) is provided in the rotating shaft (41), a plurality of liquid outlet holes (412) are provided on the outer wall of the insertion portion of the rotating shaft (41), the liquid outlet holes (412) are connected to the liquid outlet channel (411), a pipeline at the bottom of the liquid outlet channel (411) is connected to the liquid outlet (13), and a first control valve (131) is installed on the connecting pipeline from the liquid outlet channel (411) to the liquid outlet (13).

4. The sample loading processing device of a chromatography system according to claim 2, characterized in that: The power mechanism (5) comprises a motor (51), a first transmission bevel gear (53) and a second transmission bevel gear (54); an active bevel gear (52) is installed at the output end of the motor (51); the first transmission bevel gear (53) is fixedly installed at the bottom of the sample liquid processor (3); the second transmission bevel gear (54) is fixedly installed on the outer wall of the rotating shaft (41); the first transmission bevel gear (53) and the second transmission bevel gear (54) are symmetrically installed; the first transmission bevel gear (53) and the second transmission bevel gear (54) are meshed with the active bevel gear (52); and the active bevel gear (52) can drive the first transmission bevel gear (53) and the second transmission bevel gear (54) to rotate synchronously in opposite directions.

5. The sample loading processing device of a chromatography system according to claim 1, characterized in that: The sample liquid processor (3) is made of heat-conducting material, and the outer wall of the sample liquid processor (3) fits in the inner cavity of the heating seat (2).

6. The sample loading processing device of a chromatography system according to claim 1, characterized in that: A defoaming layer (31) is fixedly installed in the inner cavity of the sample liquid processor (3); the defoaming layer (31) is made of a grid screen; and a gap is left between the defoaming layer (31) and the inner wall of the sample liquid processor (3).

7. The sample loading processing device of a chromatography system according to claim 3, characterized in that: The heating seat (2) and the sample liquid processor (3) are installed in the middle section of the box body (1); a monitoring area (12) is provided on the top of the box body (1); a temperature measuring element (121) and a liquid level monitor (122) are installed on the monitoring area (12); the liquid level monitor (122) is installed with a probe (1221) extending downward; the probe (1221) extends into the cavity of the sample liquid processor (3); a waste liquid area (14) is provided at the bottom of the box body (1); a bottom pipeline of the liquid outlet channel (411) is connected to the waste liquid area (14); a second control valve (141) is installed on the connecting pipeline between the liquid outlet channel (411) and the waste liquid area (14); the first control valve (131) and the second control valve (141) are not opened at the same time.

8. The sample loading processing device of a chromatography system according to claim 7, characterized in that: An annular fixing seat (15) is installed in the box body (1), and the fixing seat (15) is arranged between the monitoring area (12) and the sample liquid processor (3). A plurality of spray nozzles (151) are evenly distributed along the annular length direction of the fixing seat (15), and the spray nozzles (151) are arranged facing the inner wall of the sample liquid processor (3). A water storage area (16) is provided at the bottom of the box body (1), and the water storage area (16) is connected to the spray nozzles (151) through a pipeline. An ultraviolet lamp (123) is installed directly above the sample liquid processor (3).

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