A device and process for extracting protease preparation from microorganisms

Abstract

The invention discloses a device for extracting a protease preparation from a microorganism, comprising: a shell, a feed pipe is fixedly installed at a central position above the shell, and a guide column is fixedly mounted inside the feed pipe; a centrifugal assembly is arranged in the shell; the centrifugal assembly comprises a turntable, a plurality of first centrifugal grooves and second centrifugal grooves are arranged on the upper surface of the turntable at intervals, the ends of the first centrifugal grooves and the second centrifugal grooves close to the axis are both connected to the sleeve, and the ends of the first centrifugal grooves and the second centrifugal grooves away from the axis are both provided with discharge holes that penetrate to the outside of the turntable; compared with the prior art, the invention realizes efficient and continuous extraction of protease preparations from microorganisms.

Description

Technical Field

[0001] The invention relates to the technical field of protease extraction, in particular to a device and process for extracting a protease preparation from microorganisms. Background Art

[0002] Protease preparations from microorganisms, as an important type of biological enzyme preparations, are widely used in many fields such as food, medicine, and chemical industry. This type of enzyme preparation mainly comes from various microorganisms, such as bacteria and fungi, and is produced through fermentation, culture, extraction, and purification. Microbial proteases have the characteristics of high efficiency, specificity, and mildness. They can catalyze the hydrolysis of proteins under mild conditions. Therefore, they are widely used in tenderization, deodorization, and debittering processes in food processing, as well as protein drug preparation in the pharmaceutical field and protein hydrolyzate production in the chemical industry.

[0003] At present, the extraction of protease preparations from microorganisms mainly relies on centrifugal extraction technology. Centrifugal extraction technology separates microbial cells from proteases in the culture medium through the action of centrifugal force. In the prior art, the implementation methods of centrifugal extraction mainly include the following:

[0004] Intermittent centrifuge: With intermittent operation, the microbial culture solution is added to the centrifuge in batches, and the cells and proteases are separated by centrifugation. However, intermittent centrifuges need to be operated in batches, which is not only cumbersome to operate, but also has low production efficiency. However, it is difficult to maintain the continuity of the centrifugation process. Once the centrifuge stops, the cells and proteases in the solution may be remixed, resulting in reduced product purity.

[0005] Continuous centrifuge: Through the continuous input and output of microbial culture solution, the centrifugal extraction process is continuous. Although the production efficiency is improved, it is often difficult for the continuous centrifuge to maintain airtightness during the centrifugation process, resulting in the premature discharge of the solution that has not been separated, affecting the purity of the product and increasing the difficulty and cost of subsequent processing.

[0006] Therefore, it is necessary to provide a device and process for extracting protease preparations from microorganisms to solve the problems raised in the above background technology. Summary of the invention

[0007] To achieve the above object, the present invention provides the following technical solution: a device for extracting a protease preparation from a microorganism, comprising:

[0008] A housing, wherein a feed pipe is fixedly mounted at a central position above the housing, and a guide column is fixedly mounted inside the feed pipe;

[0009] a centrifugal assembly disposed in the housing;

[0010] The centrifugal assembly comprises a turntable, wherein a plurality of first centrifugal grooves and a second centrifugal groove are provided on the upper surface of the turntable at intervals, wherein the ends of the first centrifugal grooves and the second centrifugal grooves close to the axis are both connected to the sleeve, and the ends of the first centrifugal grooves and the second centrifugal grooves away from the axis are both provided with discharge holes extending to the outside of the turntable;

[0011] A sleeve is fixed above the rotating disk and is rotatably connected to the inner wall of the feeding tube, wherein the outer wall of the guide column and the inner wall of the sleeve are both fixedly provided with multiple circles of crushing teeth for crushing the microbial solution;

[0012] A discharge ring is slidably arranged on the outer wall of the turntable, and the discharge ring is lifted and connected to the bottom of the shell, and the inner wall of the discharge ring is respectively provided with an upper discharge trough and a lower discharge trough, and the upper discharge trough and the lower discharge trough are respectively connected with a conduit to respectively discharge the broken cell wall impurities and the protease solution after centrifugation;

[0013] A driving motor is arranged at the bottom of the housing and connected to the turntable, and is used for driving the turntable to rotate.

[0014] Furthermore, the guide column is in the shape of a truncated cone with a diameter increasing from top to bottom.

[0015] Furthermore, a center ring is rotatably arranged at the bottom of the sleeve, and a plurality of feed holes are opened on the side wall of the center ring. The feed holes enable the sleeve to be closed with the second centrifugal groove when it is connected with the first centrifugal groove, and to be closed with the first centrifugal groove when it is connected with the second centrifugal groove.

[0016] Furthermore, a cover plate is rotatably provided on the upper surface of the turntable, the cover plate is fixedly connected to the center ring, the cover plate seals the upper surface of the turntable, and a plurality of exhaust holes and air inlet holes are circumferentially distributed in the cover plate.

[0017] Furthermore, each of the discharge holes is provided with a plug capable of sealing the discharge hole.

[0018] Furthermore, the plug is fixed in the connecting plate, sliding shafts are fixed on both sides of the connecting plate, and the sliding shafts are slidably embedded in the side walls of the turntable.

[0019] Furthermore, each of the sliding shafts passes through the sliding groove at the bottom of the turntable, and the sliding shafts on both sides of the first centrifugal groove are fixed to the first sliding block, and the sliding shafts on both sides of the second centrifugal groove are fixed to the second sliding block;

[0020] The first slider and the second slider are slidably connected to the bottom of the turntable;

[0021] Each of the first sliders is hinged with a first connecting rod, each of the first connecting rods is hinged to a first connecting ring, the bottom of the first connecting ring is rotatably connected to a first lifting ring, and the first lifting ring is liftably connected to the bottom of the housing;

[0022] Each of the second sliding blocks is hinged with a second connecting rod, each of the second connecting rods is hinged in a second connecting ring, the bottom of the second connecting ring is rotatably connected with a second lifting ring, and the second lifting ring is liftably connected to the bottom of the housing.

[0023] Furthermore, two grooves are symmetrically provided on the upper surface of the turntable, an electromagnet is fixed in the groove, a guide block embedded in the groove is fixed at the bottom of the cover plate, the guide block is made of magnetic conductive material, and a spring is provided between the guide block and the electromagnet.

[0024] A process for extracting a protease preparation from a microorganism, comprising:

[0025] S1. Continuously pour the microbial solution into the feed pipe, start the drive motor, and make the centrifugal assembly and the casing start to rotate. At this time, the crushing teeth are interlocked with each other to shear and crush the microbial solution;

[0026] S2. Under the action of centrifugal force, the microbial solution flows from the gap between the sleeve and the guide column into the first centrifugal groove or the second centrifugal groove in the centrifugal assembly;

[0027] S3, by turning the electromagnet on and off, the rotation of the cover plate is controlled, thereby changing the position of the center ring, so that the microbial solution enters the first centrifugal tank and the second centrifugal tank in sequence for centrifugal treatment;

[0028] S4. When the centrifugation of the microbial solution in the first centrifugal tank or the second centrifugal tank is completed, the plug of the corresponding discharge hole is slid open by controlling the lifting of the first lifting ring or the second lifting ring, and the broken cell wall impurities are first discharged into the upper discharge tank, and then the protease solution is discharged into the lower discharge tank by descending the discharge ring.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] In the present invention, multiple circles of crushing teeth are fixed on the guide column and the inner wall of the sleeve. When the driving motor is started, the rotation of the centrifugal assembly and the sleeve causes the crushing teeth to interlock with each other, thereby performing efficient shear crushing on the microbial solution, which not only accelerates the crushing of microbial cells, but also promotes the uniform release of proteases in the solution, thereby improving the extraction efficiency.

[0031] In the present invention, a first centrifugal groove and a second centrifugal groove are alternately arranged on the turntable in the centrifugal assembly. By rotating the center ring, the microbial solution can enter the two centrifugal grooves in sequence for centrifugal treatment, which not only realizes a continuous centrifugal separation process, but also effectively avoids blockage and accumulation problems during the centrifugation process by alternating the use of the centrifugal grooves, thereby further improving the separation efficiency.

[0032] In the present invention, the exhaust holes and the air inlet holes in the cover plate are respectively used for exhausting the microbial solution when it enters the centrifugal tank and for intake of the solution after the centrifugation is completed, so as to ensure the atmospheric pressure balance in the centrifugal tank, effectively avoid splashing or clogging of the solution caused by pressure changes, and the air can play a cleaning and unblocking role when entering and exiting the first centrifugal tank and the second centrifugal tank, further ensuring the stability and safety of the extraction process. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 The schematic diagram is a schematic diagram of the overall structure of a protease preparation extraction device derived from microorganisms;

[0034] Figure 2 It is a schematic diagram of the cross-sectional structure of the centrifugal assembly;

[0035] Figure 3 It is a schematic diagram of the structure of the upper surface of the turntable in the centrifugal assembly;

[0036] Figure 4 It is a schematic diagram of the structure inside the turntable in the centrifugal assembly;

[0037] Figure 5 It is a schematic diagram of the structure of the bottom of the turntable in the centrifugal assembly;

[0038] In the figure: 1. shell; 2. feed pipe; 3. guide column; 4. centrifugal assembly; 401. turntable; 402. first centrifugal slot; 403. second centrifugal slot; 404. discharge hole; 405. plug; 406. connecting plate; 407. sliding shaft; 408. center ring; 409. feed hole; 410. cover plate; 411. exhaust hole; 412. air inlet hole; 413. first slider; 414. first connecting rod; 415. first connecting ring; 416. first lifting ring; 417. second slider; 418. second connecting rod; 419. second connecting ring; 420. second lifting ring; 421. groove; 422. electromagnet; 423. guide block; 424. spring; 5. sleeve; 6. crushing tooth; 7. discharge ring; 701. upper discharge trough; 702. lower discharge trough; 8. driving motor. DETAILED DESCRIPTION

[0039] See also Figure 1-Figure 5 In an embodiment of the present invention, a device for extracting a protease preparation from a microorganism is characterized by comprising:

[0040] A housing 1, a feed pipe 2 is fixedly mounted at the center of the housing 1, and a guide column 3 is fixedly mounted inside the feed pipe 2;

[0041] A centrifugal assembly 4 is disposed in the housing 1;

[0042] The centrifugal assembly 4 includes a turntable 401, and a plurality of first centrifugal grooves 402 and second centrifugal grooves 403 are provided on the upper surface of the turntable 401 at intervals. The ends of the first centrifugal grooves 402 and the second centrifugal grooves 403 close to the axis are both connected to the sleeve 5, and the ends of the first centrifugal grooves 402 and the second centrifugal grooves 403 away from the axis are both provided with discharge holes 404 that penetrate to the outside of the turntable 401;

[0043] A sleeve 5 is fixed above the rotating disk 401 and is rotatably connected to the inner wall of the feeding tube 2, wherein the outer wall of the guide column 3 and the inner wall of the sleeve 5 are both fixedly provided with multiple circles of crushing teeth 6 for crushing the microbial solution;

[0044] A discharge ring 7 is slidably arranged on the outer wall of the turntable 401, and the discharge ring 7 is lifted and connected to the bottom of the housing 1, and an upper discharge trough 701 and a lower discharge trough 702 are respectively arranged on the inner wall of the discharge ring 7, and the upper discharge trough 701 and the lower discharge trough 702 are respectively connected to conduits to respectively discharge the broken cell wall impurities and the protease solution after centrifugation;

[0045] The driving motor 8 is disposed at the bottom of the housing 1 and connected to the turntable 401 , and is used for driving the turntable 401 to rotate.

[0046] The driving motor 8 drives the turntable 401 and the sleeve 5 to rotate, so that the guide column 3 and the crushing teeth 6 connected in the sleeve 5 are interlocked with each other, and the microbial solution flows into the turntable 401 from between the sleeve 5 and the guide column 3. When the microbial solution passes through the crushing teeth 6, it is sheared and crushed by the crushing teeth 6 and mixed evenly with the solution, so that the protease in the microorganism is released into the solution, and after the solution and the cell wall are separated by the centrifugal component 4, the broken cell wall impurities and the protease solution are discharged from the upper discharge trough 701 or the lower discharge trough 702 respectively by adjusting the height of the discharge ring 7.

[0047] In this embodiment, the guide column 3 is a truncated cone with a diameter increasing from top to bottom, so that the microbial solution is subject to a gradually decreasing space restriction before entering the centrifugal assembly 4, thereby promoting the initial crushing and uniform distribution of the microbial solution.

[0048] In this embodiment, a center ring 408 is rotatably provided at the bottom of the sleeve 5, and a plurality of feed holes 409 are opened on the side wall of the center ring 408. The feed holes 409 allow the sleeve 5 to be closed with the second centrifugal groove 403 when it is connected to the first centrifugal groove 402, and to be closed with the first centrifugal groove 402 when it is connected to the second centrifugal groove 403.

[0049] That is, by rotating the center ring 408 , the microbial solution in the sleeve 5 can enter the first centrifugal groove 402 and the second centrifugal groove 403 in sequence, so that the centrifugation processes in the first centrifugal groove 402 and the second centrifugal groove 403 are performed alternately.

[0050] In this embodiment, a cover plate 410 is rotatably provided on the upper surface of the turntable 401. The cover plate 410 is fixedly connected to the center ring 408. The cover plate 410 seals the upper surface of the turntable 401. The cover plate 410 has a plurality of exhaust holes 411 and air inlet holes 412 distributed circumferentially.

[0051] The exhaust hole 411 makes the feed hole 409 communicate with the first centrifugal groove 402 or the second centrifugal groove 403, and the exhaust hole 411 also communicates with the corresponding first centrifugal groove 402 or the second centrifugal groove 403, so that when the microbial solution enters the first centrifugal groove 402 or the second centrifugal groove 403, the air can be discharged from the exhaust hole 411 to balance the atmospheric pressure;

[0052] The air inlet 412 allows the center ring 408 to close the first centrifugal groove 402 or the second centrifugal groove 403, and the air inlet 412 is connected to the corresponding first centrifugal groove 402 or the second centrifugal groove 403, so that when the microbial solution in the first centrifugal groove 402 or the second centrifugal groove 403 is discharged from the discharge hole 404, air can enter from the air inlet 412 to balance the atmospheric pressure.

[0053] In this embodiment, each of the discharge holes 404 is provided with a plug 405 which can seal the discharge hole 404 .

[0054] In this embodiment, the plug 405 is fixed in the connecting plate 406 , and sliding shafts 407 are fixed on both sides of the connecting plate 406 . The sliding shafts 407 are slidably embedded in the side walls of the rotating disk 401 .

[0055] That is, the plug 405 can close the discharge hole 404 by sliding the connecting plate 406 , so that after the microbial solution in the first centrifugal tank 402 or the second centrifugal tank 403 is centrifuged, the solution and cell walls are discharged from the upper discharge tank 701 or the lower discharge tank 702 .

[0056] In this embodiment, each of the sliding shafts 407 passes through the sliding groove at the bottom of the rotating disk 401, and the sliding shafts 407 on both sides of the first centrifugal groove 402 are fixed to the first sliding block 413, and the sliding shafts 407 on both sides of the second centrifugal groove 403 are fixed to the second sliding block 417;

[0057] The first slider 413 and the second slider 417 are slidably connected to the bottom of the rotating disk 401;

[0058] Each of the first sliders 413 is hinged with a first connecting rod 414, and each of the first connecting rods 414 is hinged to a first connecting ring 415. The bottom of the first connecting ring 415 is rotatably connected to a first lifting ring 416, and the first lifting ring 416 is liftably connected to the bottom of the housing 1;

[0059] Each second slider 417 is hinged with a second connecting rod 418 , and each second connecting rod 418 is hinged with a second connecting ring 419 . The bottom of the second connecting ring 419 is rotatably connected with a second lifting ring 420 . The second lifting ring 420 is liftably connected to the bottom of the housing 1 .

[0060] That is, by controlling the lifting of the first lifting ring 416 and the second lifting ring 420 , the discharge hole 404 of each first centrifugal groove 402 or the second centrifugal groove 403 can be controlled to be opened or closed respectively.

[0061] In this embodiment, two grooves 421 are symmetrically provided on the upper surface of the turntable 401, and an electromagnet 422 is fixed in the groove 421. A guide block 423 embedded in the groove 421 is fixed at the bottom of the cover plate 410. The guide block 423 is made of magnetic conductive material, and a spring 424 is arranged between the guide block 423 and the electromagnet 422.

[0062] That is to say, by turning the electromagnet 422 on and off, the cover plate 410 can be controlled to rotate to a position where the guide block 423 is close to or away from the electromagnet 422, and this position enables the feed hole 409 in the center ring 408 to be connected to the first centrifugal groove 402 or the second centrifugal groove 403, thereby controlling the microbial solution in the sleeve 5 to enter the first centrifugal groove 402 or the second centrifugal groove 403.

[0063] A process for extracting a protease preparation from a microorganism, comprising:

[0064] The microbial solution is continuously poured into the feed pipe 2, and the drive motor 8 is started to rotate the centrifugal assembly 4 and the sleeve 5. At this time, the crushing teeth 6 are interlocked with each other to shear and crush the microbial solution;

[0065] Under the action of centrifugal force, the microbial solution flows from the gap between the sleeve 5 and the guide column 3 into the first centrifugal groove 402 or the second centrifugal groove 403 in the centrifugal assembly 4 depending on the position of the center ring 408;

[0066] By turning the electromagnet 422 on and off, the rotation of the cover plate 410 is controlled, thereby changing the position of the center ring 408, so that the microbial solution enters the first centrifugal tank 402 and the second centrifugal tank 403 in sequence for centrifugal treatment;

[0067] When the centrifugation of the microbial solution in the first centrifugal trough 402 or the second centrifugal trough 403 is completed, the plug 405 of the corresponding discharge hole 404 is slid open by controlling the lifting of the first lifting ring 416 or the second lifting ring 420, and the broken cell wall impurities are first discharged into the upper discharge trough 701, and then the protease solution is discharged into the lower discharge trough 702 by descending the discharge ring.

[0068] By centrifuging in the first centrifugal tank 402 and the second centrifugal tank 403 in turn, and discharging the broken cell wall impurities and the protease solution in turn through the upper discharge tank 701 and the lower discharge tank 702 respectively, continuous centrifugation can be performed.

[0069] What is described above is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A device for extracting protease preparations from microorganisms, characterized in that: include: A housing, a feed pipe is fixedly mounted at the center of the housing, and a guide column is fixedly mounted inside the feed pipe; a centrifugal assembly disposed in the housing; The centrifugal assembly comprises a turntable, wherein a plurality of first centrifugal grooves and a second centrifugal groove are provided on the upper surface of the turntable at intervals, wherein the ends of the first centrifugal grooves and the second centrifugal grooves close to the axis are both connected to the sleeve, and the ends of the first centrifugal grooves and the second centrifugal grooves away from the axis are both provided with discharge holes extending to the outside of the turntable; A sleeve is fixed above the rotating disk and is rotatably connected to the inner wall of the feeding tube, wherein the outer wall of the guide column and the inner wall of the sleeve are both fixedly provided with multiple circles of crushing teeth for crushing the microbial solution; A discharge ring is slidably arranged on the outer wall of the turntable, and the discharge ring is lifted and connected to the bottom of the shell, and the inner wall of the discharge ring is respectively provided with an upper discharge trough and a lower discharge trough, and the upper discharge trough and the lower discharge trough are respectively connected with a conduit to respectively discharge the broken cell wall impurities and the protease solution after centrifugation; A driving motor, disposed at the bottom of the housing and connected to the turntable, for driving the turntable to rotate; A center ring is rotatably arranged at the bottom of the sleeve, and a plurality of feed holes are opened on the side wall of the center ring. The feed holes make the sleeve closed with the second centrifugal groove when it is connected with the first centrifugal groove, and closed with the first centrifugal groove when it is connected with the second centrifugal groove.

2. The device for extracting a protease preparation from a microorganism according to claim 1, characterized in that: The guide column is in the shape of a truncated cone with a diameter increasing from top to bottom.

3. The device for extracting a protease preparation from a microorganism according to claim 2, characterized in that: A cover plate is rotatably provided on the upper surface of the turntable, and the cover plate is fixedly connected to the center ring. The cover plate seals the upper surface of the turntable, and a plurality of exhaust holes and air inlet holes are circumferentially distributed in the cover plate.

4. The device for extracting a protease preparation from a microorganism according to claim 1, characterized in that: Each of the discharge holes is provided with a plug capable of sealing the discharge hole.

5. The device for extracting a protease preparation from a microorganism according to claim 4, characterized in that: The plug is fixed in the connecting plate, sliding shafts are fixed on both sides of the connecting plate, and the sliding shafts are slidably embedded in the side walls of the rotating disk.

6. The device for extracting a protease preparation from a microorganism according to claim 5, characterized in that: Each of the sliding shafts passes through the sliding groove at the bottom of the turntable, and the sliding shafts on both sides of the first centrifugal groove are fixed to the first sliding block, and the sliding shafts on both sides of the second centrifugal groove are fixed to the second sliding block; The first slider and the second slider are slidably connected to the bottom of the turntable; Each of the first sliders is hinged with a first connecting rod, each of the first connecting rods is hinged to a first connecting ring, the bottom of the first connecting ring is rotatably connected to a first lifting ring, and the first lifting ring is liftably connected to the bottom of the housing; Each of the second sliding blocks is hinged with a second connecting rod, each of the second connecting rods is hinged in a second connecting ring, the bottom of the second connecting ring is rotatably connected with a second lifting ring, and the second lifting ring is liftably connected to the bottom of the housing.

7. The device for extracting a protease preparation from a microorganism according to claim 3, characterized in that: The upper surface of the turntable is symmetrically provided with two grooves, in which electromagnets are fixed, and the bottom of the cover plate is fixed with a guide block embedded in the groove, the guide block is made of magnetic conductive material, and a spring is arranged between the guide block and the electromagnet.

8. A process for extracting a protease preparation from a microorganism, using the device for extracting a protease preparation from a microorganism according to claim 7, characterized in that: include: S1. Continuously pour the microbial solution into the feed pipe, start the drive motor, and make the centrifugal assembly and the casing start to rotate. At this time, the crushing teeth are interlocked with each other to shear and crush the microbial solution; S2. Under the action of centrifugal force, the microbial solution flows from the gap between the sleeve and the guide column into the first centrifugal groove or the second centrifugal groove in the centrifugal assembly; S3, by turning the electromagnet on and off, the rotation of the cover plate is controlled, thereby changing the position of the center ring, so that the microbial solution enters the first centrifugal tank and the second centrifugal tank in sequence for centrifugal treatment; S4. When the centrifugation of the microbial solution in the first centrifugal tank or the second centrifugal tank is completed, the plug of the corresponding discharge hole is slid open by controlling the lifting of the first lifting ring or the second lifting ring, and the broken cell wall impurities are first discharged into the upper discharge tank, and then the protease solution is discharged into the lower discharge tank by descending the discharge ring.

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