Microscope glass slide three-point leveling device

Abstract

The invention provides a three-point leveling device for a microscope glass slide. The three-point leveling device comprises a slide way fixing plate, a proximity switch, an adjusting side plate, a magnetic iron piece, a magnetic stone, a glass slide slide way, a phytoplankton standard counting frame, a cover glass, a pull-out piece, a pin rod, a bottom adjusting seat, a fine thread adjuster mounting seat, a fine thread adjuster, a tension spring, a wave piece, a set screw and a wedge-shaped upper end adjusting plate. The flatness of the wedge-shaped upper end adjusting plate is adjusted through the three fine thread adjusters, meanwhile, it is guaranteed that the tension spring can be in a stretching state, and the requirement for long-term use of an instrument in various environments and various platforms can be met only through one-time leveling adjustment.

Description

Technical Field

[0001] The invention belongs to the technical field of microscopic imaging monitoring and relates to a three-point leveling device for a microscope slide. Background Art

[0002] At present, manual microscopic examination is still the main means of identifying plankton species, but this method requires experienced identification personnel, has a long detection cycle, and cannot solve the problem of large-scale and high-frequency monitoring in ecological surveys. Computer-assisted image recognition technology combines morphological identification methods with expert databases, which can effectively reduce the dependence of identification work on professionals and improve the efficiency of plankton identification. It has become a research hotspot in this field. At the same time, the acquisition of high-resolution images is the basis for ensuring high identification efficiency.

[0003] Microscopic imaging system is the basis for obtaining high-resolution images. Microscopic observation can only observe one plane. When the observation stage is not perpendicular to the objective lens, the observed sample is not at the same focal length, which easily causes the observed image to be clear on one side and blurred on the other. By designing the leveling structure of the stage to ensure that the lens is perpendicular to the observation slide, a good observation effect can be obtained. Summary of the invention

[0004] In order to achieve the above-mentioned purpose, the present invention designs a three-point leveling device for a microscope slide, comprising: a slide fixing plate, a proximity switch, an adjustment side plate, a magnetic iron sheet, a magnetic stone, a slide slide, a phytoplankton standard counting frame, a cover glass, a pull-out sheet, a pin, a bottom adjustment seat, a fine-thread adjuster mounting seat, a fine-thread adjuster, a tension spring, a ball, a set screw, and a wedge-shaped upper end adjustment plate; The cam is threaded with a thread on the inside of the adjusting seat, and the fine-thread adjuster is screwed into the fine-thread adjuster mounting seat through the thread. The ball is placed in the circular groove on the upper end surface of the bottom adjusting seat, and the pin rod passes through the side end hole of the wedge-shaped upper end adjusting plate and the hook eye at one end of the tension spring to fix the tension spring in the four-end concave holes on the wedge-shaped upper end adjusting plate. The fine-thread adjuster is twisted to support the circular groove corresponding to the bottom end of the wedge-shaped upper end adjusting plate so that the distance between the wedge-shaped upper end adjusting plate and the bottom adjusting seat can keep the tension spring in a stretched state. The other pin rod passes through the hook eye at the other end of the tension spring and the end hole of the wedge-shaped upper end adjusting plate so that the tension spring passes through the wedge-shaped upper end adjusting plate The four concave holes at the end of the bottom adjustment seat corresponding to the concentric ends of the end adjustment plate fix the tension spring between the wedge-shaped upper end adjustment plate and the bottom adjustment seat. At the same time, the ball is also located at the concentric corresponding place between the circular groove on the upper end surface of the wedge-shaped upper end adjustment plate and the circular groove on the lower end surface of the bottom adjustment seat. The magnetic stone is controlled by processing tolerance to achieve interference fit between the magnetic stone and the groove on the side end of the slide glass slide, thereby achieving compact assembly. The pull-out sheet is fixed to the slide glass slide through a thread, the standard phytoplankton counting frame is placed in the groove of the slide glass slide, the cover glass is placed above the standard phytoplankton counting frame, the adjustment side plate is fixed to the bottom adjustment seat through a thread, and the height of the wedge-shaped upper end adjustment plate after assembly is measured, the magnetic sheet is glued to the adjustment side plate, the slide fixing plate is fixed to the adjustment side plate through a thread, and the proximity switch is fixed to the slide fixing plate.

[0005] The present invention has the following beneficial effects: (1) The flatness of the wedge-shaped upper adjustment plate is adjusted by three fine-tooth adjusters, while ensuring that the tension spring can be in a stretched state. Only one leveling adjustment is required to meet the long-term use of the instrument in various environments and platforms.

[0006] (2) The wedge-shaped upper adjustment plate and the slide slide are designed with a dovetail groove to prevent the slide from colliding with the objective lens due to human operating errors. BRIEF DESCRIPTION OF THE DRAWINGS

[0007] Figure 1 This is an overall assembly diagram of the three-point leveling device for a microscope slide of the present invention; Figure 2 It is the assembly diagram of the three-point leveling device of the present invention; Figure 3 This is a diagram of the assembly of the slide of the present invention.

[0008] In the figure, the reference numerals are: slide fixing plate 1, proximity switch 2, adjustment side plate 3, magnetic iron sheet 4, magnetic stone 5, slide glass 6, phytoplankton standard counting frame 7, cover glass 8, pull-out sheet 9, pin 10, bottom adjustment seat 11, fine-tooth adjuster mounting seat 12, fine-tooth adjuster 13, tension spring 14, ball 15, set screw 16, wedge-shaped upper end adjustment plate 17. DETAILED DESCRIPTION

[0009] In order to make the purpose, technical scheme and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention. In addition, the technical features involved in each embodiment of the present invention described below can be combined with each other as long as they do not conflict with each other. To achieve the above-mentioned purpose, the present invention adopts the following technical scheme.

[0010] To achieve the above object, the present invention designs a three-point leveling device for a microscope slide, such as Figure 1 As shown, it includes: a slide fixing plate 1, a proximity switch 2, an adjustment side plate 3, a magnetic iron sheet 4, a magnetic stone 5, a slide glass slide 6, a phytoplankton standard counting frame 7, a cover glass 8, a pull-out sheet 9, a pin 10, a bottom adjustment seat 11, a fine-tooth adjuster mounting seat 12, a fine-tooth adjuster 13, a tension spring 14, a ball 15, a set screw 16, and a wedge-shaped upper end adjustment plate 17.

[0011] like Figure 2 , Figure 3As shown, the bottom adjustment seat 11 is placed at the bottom, the fine-thread adjuster mounting seat 12 is concentrically assembled with the three mounting holes of the bottom adjustment seat 11, the side end step of the fine-thread adjuster mounting seat 12 is flush with the bottom adjustment seat 11 and is fixed by a set screw 16; the fine-thread adjuster mounting seat 12 has threads inside, the fine-thread adjuster 13 is screwed into the fine-thread adjuster mounting seat 12 through threads, the ball 15 is placed in the circular groove on the upper end surface of the bottom adjustment seat 11, the pin rod 10 passes through the side end hole of the wedge-shaped upper end adjustment plate 17 and the hook eye at one end of the tension spring 14 to fix the tension spring 14 in the four-end concave holes on the wedge-shaped upper end adjustment plate 17. The fine-tooth adjuster 13 is twisted to press against the circular groove corresponding to the bottom end of the wedge-shaped upper end adjustment plate 17 so that the distance between the wedge-shaped upper end adjustment plate 17 and the bottom adjustment seat 11 can keep the tension spring 14 in a stretched state. The other hook eye of the tension spring 14 is pulled by tweezers to make the tension spring 14 pass through the four-end concave holes of the bottom adjustment seat 11 corresponding to the concentricity of the wedge-shaped upper end adjustment plate 17. At this time, another pin rod 10 is passed through the side end hole of the bottom adjustment seat 11 and the other hook eye of the tension spring 14. The tweezers are released. The tension spring 14 is kept in a stretched state under the action of the pin rods at both ends. The tension spring 14 is fixed between the wedge-shaped upper end adjustment plate 17 and the bottom adjustment seat 11. At the same time, the ball 15 is also located at the concentric corresponding place of the circular groove on the upper end surface of the wedge-shaped upper end adjustment plate 17 and the circular groove on the lower end surface of the bottom adjustment seat 11. The magnetic absorber 5 is made to achieve an interference fit with the side end groove of the slide slide 6 by controlling the processing tolerance, thereby achieving compact assembly. The pull-out sheet 9 is fixed to the slide glass slide 6 by a thread. The phytoplankton standard counting frame 7 is placed in the groove of the slide glass slide 6, and the cover glass 8 is placed above the phytoplankton standard counting frame 7. The adjusting side plate 3 is fixed to the bottom adjusting seat 11 by a thread. By measuring the height of the wedge-shaped upper end adjusting plate 17 after assembly, the magnetic iron sheet 4 is glued to the adjusting side plate 3, the slide fixing plate 1 is fixed to the adjusting side plate 3 by a thread, and the proximity switch 2 is fixed to the slide fixing plate 1 by a screw.

[0012] During operation, 0.1 mL of the liquid sample to be tested is dropped onto the phytoplankton standard counting frame 7, covered with a cover glass 8, and slid into the upper dovetail notch of the wedge-shaped upper end adjustment plate 17 through the dovetail notch at the bottom of the slide slide 6, and fixed by the mutual attraction between the magnetic absorber 5 and the magnetic absorber sheet 4.

[0013] Furthermore, to ensure that the glass slide 6 can be easily inserted into the wedge-shaped upper end adjustment plate 17, the wedge-shaped groove part of the glass slide 6 takes a negative tolerance during processing, and the wedge-shaped groove part of the wedge-shaped upper end adjustment plate 17 takes a positive tolerance. The purpose of designing the wedge-shaped groove is to prevent the glass slide 6 from tilting upward and hitting the microscope objective during the sliding process. Therefore, the wedge-shaped groove angle is recommended to be designed at about 20 degrees, which can avoid assembly jams caused by too small an angle and avoid failure of anti-collision effect due to too large an angle. At the same time, in order to facilitate the sliding of the glass slide 6 during operation, a large-angle chamfer design is performed on the entrance.

[0014] Furthermore, the thread size of the fine thread adjuster 13 is M4 0.25, the adjustment stroke is 4mm.

[0015] Furthermore, in order to ensure that the magnetic stone 5 can generate a greater force with the magnetic iron sheet 4, the material of the magnetic stone 5 is a neodymium magnet.

[0016] Furthermore, the number of the tension springs 14 is 4, and the number of the fine-thread adjusters 13 is 3.

[0017] Furthermore, the size of the phytoplankton standard counting frame 7 is 76 mm 38mm, the size available for microscopic observation is 20mm 20mm.

[0018] Furthermore, the function of the ball 15 limits the left-right deviation of the wedge-shaped upper end adjustment plate 17 and the bottom adjustment seat 11, and at the same time protects the tension spring 14 from excessive extension and loss of elasticity.

[0019] The device is assembled on a 3D scanning platform and used in conjunction with the scanning platform. Due to the tolerances generated during the processing and assembly process, when the glass slide is directly placed on it for use, there will be a height difference of about 200-500 microns on both sides. Since the depth of field of the microscope objective is only a few microns, the scanning imaging process causes image blur, and multiple focusing is required, which affects the measurement time. Through the three-point leveling device, a height difference of less than 30 microns can be achieved on both sides of the glass slide. By clicking the leveling test through the software, the 3D scanning platform automatically moves to nine points around the microscope slide, with the lower right corner as the reference point. The remaining eight points are subtracted from the reference point to obtain the value, and the maximum value of the nine-point measurement value minus the minimum value is used to indicate the leveling degree.

Claims

1. A three-point leveling device for a microscope slide, characterized in that: include: Slide fixing plate, proximity switch, adjustment side plate, magnetic iron sheet, magnetic stone, slide glass, phytoplankton standard counting frame, cover glass, pull-out sheet, pin rod, bottom adjustment seat, fine-thread adjuster mounting seat, fine-thread adjuster, tension spring, ball, set screw, wedge-shaped upper end adjustment plate; The cam is threaded with a thread on the inside of the adjusting seat, and the fine-thread adjuster is screwed into the fine-thread adjuster mounting seat through the thread. The ball is placed in the circular groove on the upper end surface of the bottom adjusting seat, and the pin rod passes through the side end hole of the wedge-shaped upper end adjusting plate and the hook eye at one end of the tension spring to fix the tension spring in the four-end concave holes on the wedge-shaped upper end adjusting plate. The fine-thread adjuster is twisted to support the circular groove corresponding to the bottom end of the wedge-shaped upper end adjusting plate so that the distance between the wedge-shaped upper end adjusting plate and the bottom adjusting seat can keep the tension spring in a stretched state. The other pin rod passes through the hook eye at the other end of the tension spring and the end hole of the wedge-shaped upper end adjusting plate so that the tension spring passes through the wedge-shaped upper end adjusting plate The four concave holes at the end of the bottom adjustment seat corresponding to the concentric ends of the end adjustment plate fix the tension spring between the wedge-shaped upper end adjustment plate and the bottom adjustment seat. At the same time, the ball is also located at the concentric corresponding place between the circular groove on the upper end surface of the wedge-shaped upper end adjustment plate and the circular groove on the lower end surface of the bottom adjustment seat. The magnetic stone is controlled by processing tolerance to achieve interference fit between the magnetic stone and the groove on the side end of the slide glass slide, thereby achieving compact assembly. The pull-out sheet is fixed to the slide glass slide through a thread, the standard phytoplankton counting frame is placed in the groove of the slide glass slide, the cover glass is placed above the standard phytoplankton counting frame, the adjustment side plate is fixed to the bottom adjustment seat through a thread, and the height of the wedge-shaped upper end adjustment plate after assembly is measured, the magnetic sheet is glued to the adjustment side plate, the slide fixing plate is fixed to the adjustment side plate through a thread, and the proximity switch is fixed to the slide fixing plate.

2. A three-point leveling device for a microscope slide according to claim 1, characterized in that: During operation, drop the liquid sample to be tested on the standard phytoplankton counting frame, cover it with a cover glass, slide it into the dovetail notch on the upper end of the wedge-shaped upper adjustment plate through the dovetail notch at the bottom of the slide slide, and fix it by the mutual attraction between the magnetic stone and the magnetic iron sheet.

3. A three-point leveling device for a microscope slide according to claim 1, characterized in that: The wedge-shaped groove part of the slide slide takes a negative tolerance, the wedge-shaped groove part of the wedge-shaped upper end adjustment plate takes a positive tolerance, and the wedge-shaped groove angle is set at about 20 degrees.

4. A three-point leveling device for a microscope slide according to claim 1, characterized in that: The thread size of the fine thread adjuster is M4 0.25, the adjustment stroke is 4mm.

5. A three-point leveling device for a microscope slide according to claim 1, characterized in that: The material of the magnetic stone is neodymium magnet.

6. A three-point leveling device for a microscope slide according to claim 1, characterized in that: The number of the tension springs is 4, and the number of the fine-thread adjusters is 3.

7. A three-point leveling device for a microscope slide according to claim 1, characterized in that: The size of the standard phytoplankton counting frame 7 is 76mm 38mm, the size available for microscopic observation is 20mm 20mm.

8. A three-point leveling device for a microscope slide according to claim 1, characterized in that: The ball is used to limit the left and right deviation of the wedge-shaped upper adjustment plate and the bottom adjustment seat to prevent the tension spring from over-stretching and losing its elasticity.

Images