Deutzia glabrata hard wood cutting breeding method

Abstract

The invention provides a deutzia glabrata hard wood cutting breeding method, and relates to the technical field of vegetative propagation seedling raising, and the method comprises the following steps: (1) collecting 1-2-year-old branches, and trimming thin branches; (2) cutting the branches in the step (1) into cutting slips with the length of 10cm and more than two bud points, flatly cutting upper openings of the cutting slips and obliquely cutting lower openings of the cutting slips, and soaking roots of the cutting slips into a sterilizing solution for sterilization treatment; (3) selecting grass carbon mixed perlite as a cutting medium, putting the cutting medium into a plug tray, and disinfecting the plug tray; (4) soaking the cutting slips obtained in the step (2) in a rooting agent, then inserting the cutting slips into a plug tray substrate, thoroughly watering the cutting slips with a sterilizing solution, and covering the cutting slips with a transparent cover; (5) after cuttage, placing under the condition of 70% shading degree, adjusting the shading degree to be 50% and the temperature to be 10-30 DEG C after new leaves emerge, timely supplementing water after cuttage, sterilizing in combination with a sterilizing solution, gradually opening a transparent cover after 45 days of cuttage, and watering according to conditions; and (6) 60 days after cuttage, transplanting and planting. The method has the advantages that the seedling raising period is shortened, and the breeding efficiency of deutzia glabrata is improved.

Description

Technical Field

[0001] The invention provides a hard-branch cutting propagation method for Deutzia glabra, and relates to the technical field of asexual propagation and seedling raising. Background Art

[0002] Deutzia glabrata Kom., also known as Laoshan Deutzia, is a plant of the genus Deutzia in the Saxifragaceae family. It is a rare and precious tree species in Shandong Province and has important scientific research value. Its flowering period is in the early summer when there are few flowers. The flowers are white, dense and elegant. It can be planted on hillsides, lawns, roadsides or as hedges. It is an excellent garden plant in my country.

[0003] At present, there is little research data on Deutzia glabra, which mainly focuses on the fields of molecular, ecological, and seed breeding. Deutzia glabra seeds are extremely small seeds, and there are two ways to breed them: field sowing and container seedlings. Field sowing requires careful ploughing and is not easy to form seedlings; while container seedlings require a special germination device composed of a culture tray, copper foil modified asphalt roll, germination matrix layer, vermiculite layer, and river sand layer. In addition, during the germination period, after germination to the transplanting period, seedling cultivation period, winter protection, etc., a variety of different methods are required for fine maintenance. It takes 2 years to be transplanted, so the seedling cycle is long and the cost is high.

[0004] Cuttings, as an important asexual propagation technique, have been widely used in plant breeding, but the cutting propagation method of Deutzia glabra has not been reported yet. In the data on hardwood cuttings of plants of the same genus, the study by Xu Jie et al. showed that the cutting survival rate of Deutzia cabra reached 86%, the rooting rate reached 85%, and the average number of roots was 15 when the mixed matrix of perlite: vermiculite: peat 1:1:1 was used as the cutting matrix, and the rooting agent concentration was 1000mg / kg and the treatment was 30s. The hardwood cutting study conducted by He Wei et al. on Deutzia grandiflora showed that the survival rate of 2-year-old branches and the rooting rate of 500mg·L -1 The survival rate of cuttings was the highest when the cuttings were soaked in NAA for 90s and perlite was used as the substrate, reaching 89.47%; Wang Jianqiu conducted a cutting experiment on Deutzia parviflora var. amurensis, and the results showed that ABT 200ppm treatment for 2h was the most suitable cutting design, with a rooting rate of 90%, while Zhang Bihong et al.'s research on the cuttings of Deutzia parviflora showed that the best effect was achieved by treating it with ABT 300mg / L for 1h, with a rooting rate of 90%; Yuan Xue et al.'s research on the reproduction technology of Deutzia parviflora showed that its hard branch cuttings should be collected and stored in sand before the sap flows in early spring, and then treated with NAA 300mg / L for cuttings, with a rooting rate of 80.0%. Deutzia glycyrrhizae is a different plant from Deutzia glycyrrhizae, Deutzia grandiflora, Deutzia manchuria, and Deutzia small-flowered. Currently, there is a lack of technical information on the hardwood cuttings of Deutzia glycyrrhizae. Summary of the invention

[0005] The invention provides a hard-branch cutting propagation method for Deutzia glabra, which solves the technical problems in the prior art that the seedling raising period of Deutzia glabra is long and the plant can only be planted in the second year, and there is a lack of a technical solution for the hard-branch cutting of Deutzia glabra.

[0006] The present invention is achieved by comprising the steps of:

[0007] (1) Collect 1-2 year old branches and trim the thin branches;

[0008] (2) cutting the branches of step (1) into cuttings with a length of 10 cm and two or more buds, cutting the cuttings flat on the top and obliquely on the bottom, and soaking the roots of the cuttings in a sterilizing solution for sterilization;

[0009] (3) Select peat mixed with perlite as the cutting medium, put it into the plug tray and disinfect it;

[0010] (4) soaking the cuttings obtained in step (2) in a rooting agent and inserting them into a plug tray matrix, watering them thoroughly with a sterilizing solution, and covering them with a transparent cover;

[0011] (5) After cutting, place under 70% shading conditions. After the new leaves are unfolded, adjust the shading to 50% and the temperature to 10℃~30℃. Rehydrate in time after cutting and sterilize with bactericidal liquid. Remove the transparent cover gradually after 45 days of cutting and water as needed;

[0012] (6) Transplant and plant 60 days after cutting.

[0013] As further preferred, the rooting agent is one or both of NAA and IBA.

[0014] As further preferred, the NAA concentration is 1000 mg / L.

[0015] As a further preference, in step (5), the method for cutting tubes is: open the cover to investigate the rooting situation and replenish water once every 3 days, and sterilize by spraying a sterilizing liquid in combination with water replenishment every 15 days.

[0016] As a further preference, in step (3), the mass ratio of peat to perlite is 4:1.

[0017] As a further preference, the rooting agent treatment method is: soaking 3 cm from the base of the cuttings and treating for 1 minute.

[0018] As a further preference, the sterilizing solution is a 1000-fold solution of 50% carbendazim wettable powder.

[0019] The technical solution provided by the invention fills the blank of hard branch cutting propagation of Deutzia glabra, shortens the seedling raising period, and can be planted in the same year. The cutting propagation method is simple, and no complicated germination device is required, which improves the propagation efficiency of Deutzia glabra and reduces the cost of container seedling raising. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a cutting effect diagram of Example 1;

[0021] Figure 2 This is a rendering of the plug tray covered with a transparent cover after cutting in Example 1;

[0022] Figure 3 This is a diagram of the rooting situation of Example 1 when the cuttings are 30 days old;

[0023] Figure 4 This is a schematic diagram of the survival of cuttings after 60 days of cutting in Example 1;

[0024] Figure 5 This is a schematic diagram of the roots of the cuttings after 60 days of cutting in Example 1;

[0025] Figure 6 This is a schematic diagram of the root system of Example 1 after cleaning the soil at the root of the cuttings 60 days after cutting;

[0026] Figure 7 This is a schematic diagram of the root system of a single cutting 60 days after cutting in Example 1. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] Example 1

[0029] Hard branch cutting propagation method of Deutzia glabra

[0030] 1. Strip selection and pretreatment

[0031] In Jinan, Shandong, in late March, when the buds of Deutzia glabra sprouted, mother plants with good growth were selected, and 1-2 year old strong branches were collected, and branches with a thickness of less than 0.2 cm were cut off.

[0032] 2. Cuttings cutting and disinfection

[0033] The branches pretreated as above were cut into cuttings with 2 to 3 buds and a length of about 10 cm. The upper end of the cuttings was cut flat and the lower end was cut into a horseshoe-shaped oblique end. Every 50 cuttings were bundled and placed in a 1000-fold solution of carbendazim (50% wettable powder) and soaked for 5 minutes for disinfection.

[0034] 3. Preparation of cutting medium and rooting agent

[0035] Use peat: perlite = 4:1 as the cutting medium, and use 1000 times diluted carbendazim (50% wettable powder) to disinfect after placing it in the plug tray; prepare 1000 mg / L naphthaleneacetic acid (NAA) solution as a rooting agent, and store the rooting agent solution at 5°C in the dark for future use.

[0036] 4. Cuttings

[0037] Soak the cuttings treated in step 2 in the rooting agent solution to a depth of 3 cm from the base of the cuttings. Take them out after 1 minute of treatment, and then make a 4 cm deep and 1 cm diameter cutting hole in the middle of each cutting position in the plug tray. Figure 1 , put the treated cuttings into the hole, fix the cuttings, and water them thoroughly with 1000 times dilution of carbendazim (50% wettable powder). Figure 2 , each hole tray is covered with a transparent cover to maintain humidity.

[0038] 5. Management after cutting

[0039] After cutting, place the cutting tray under 70% shading conditions. After the new leaves are unfolded, adjust the shading to 50%. During the cutting period, the night temperature should not be lower than 10℃, and the daytime temperature should not be higher than 30℃. Open the cover every 3 days to check the rooting situation and add water once to keep the moisture content of the cutting medium at 50% to 70%. Every 15 days, apply 1000 times diluted carbendazim (50% wettable powder) for sterilization. The amount of water added each time depends on the moisture content of the medium, and the amount of carbendazim used depends on the amount of water added. Figure 3 , roots will begin to form 30 days after cuttings. Gradually remove the transparent cover after 45 days of cuttings. Figure 5 , Figure 6 and Figure 7 , 60 days after cutting, the seedling height, root length, root type and number, and rooting rate were investigated. The results are shown in Table 1. The seedlings can be planted in the same year. After transplanting, routine management was carried out and the survival rate reached 98%.

[0040] Table 1 Example 1 Cutting results

[0041]

[0042] As shown in Table 1, the cuttings began to take root 30 days after cutting, with an average seedling height of 15.77 cm and a root length of 10.9 cm. The rooting type was a mixed rooting type of callus rooting and cortex rooting. The final rooting rate, i.e. the cutting survival rate, was 92%, and the plants were transplanted out of the nursery.

[0043] Example 2

[0044] Compared with Example 1, the only difference is that the rooting agent used in step 3 is different. In step 3 of Example 2, the rooting agent is 1000 mg / L indolebutyric acid (IBA) solution, and the rest of the operations are the same. After 60 days of cutting, the seedling height, root length, root type and number, and rooting rate were investigated. The investigation results are shown in Table 2.

[0045] Table 2 Example 2 Cutting results

[0046]

[0047] As shown in Table 2, the cuttings began to take root 27 days after cutting, with an average seedling height of 17.11 cm and a root length of 11.4 cm. The rooting type was a mixed rooting type of callus rooting and cortex rooting. The final rooting rate, i.e. the cutting survival rate, was 86%, and the cuttings were transplanted out of the nursery.

[0048] Example 3

[0049] Compared with Example 1, the only difference is that the rooting agent used in step 3 is different. In Example 3, the rooting agent in step 3 is selected from 700 mg / L NAA+300 mg / L IBA solution, and the rest of the operations are the same. After 60 days of cutting, the seedling height, root length, root type and number, and rooting rate were investigated. The investigation results are shown in Table 3.

[0050] Table 3 Example 3 Cutting results

[0051]

[0052] As shown in Table 3, the cuttings began to take root 30 days after cutting, with an average seedling height of 15.35 cm and a root length of 11.28 cm. The rooting type was a mixed rooting type of callus rooting and cortex rooting. The final rooting rate, i.e. the cutting survival rate, was 88%, and the cuttings were transplanted out of the nursery.

[0053] Example 4

[0054] Compared with Example 1, the only difference is that the rooting agent used in step 3 is different. In Example 4, the rooting agent in step 3 is a 300 mg / L NAA+700 mg / L IBA solution prepared as a rooting agent, and the rest of the operations are the same. After 60 days of cutting, the seedling height, root length, root type and number, and rooting rate were investigated. The investigation results are shown in Table 4.

[0055] Table 4 Example 4 Cutting results

[0056]

[0057] As shown in Table 4, the cuttings began to take root 27 days after cutting, with an average seedling height of 13.89 cm and a root length of 11.53 cm. The rooting type was a mixed rooting type of callus rooting and cortex rooting. The final rooting rate, i.e. the cutting survival rate, was 86%, and the cuttings were transplanted out of the nursery.

[0058] From Examples 1-4, using 1000mg / L NAA solution, 1000mg / L IBA solution, 700mg / L NAA+300mg / L IBA solution, and 300mg / L NAA+700mg / L IBA solution as rooting agents, the rooting rates of Deutzia glabra were 92%, 86%, 88%, and 86%, respectively. When 1000mg / L NAA solution was used as the rooting hormone, the rooting rate of Deutzia glabra was the highest.

[0059] The technical solution provided by the invention fills the blank of hard branch cutting propagation of Deutzia glabra, shortens the seedling raising period, and can be planted in the same year. The cutting propagation method is simple, and no complicated germination device is required, which improves the propagation efficiency of Deutzia glabra and reduces the cost of container seedling raising.

[0060] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A hard branch cutting propagation method for Deutzia glabra, characterized in that: The steps include: (1) Collect 1-2 year old branches and trim the thin branches; (2) cutting the branches of step (1) into cuttings with a length of 10 cm and two or more buds, cutting the cuttings flat on the top and obliquely on the bottom, and soaking the roots of the cuttings in a sterilizing solution for sterilization; (3) Select peat mixed with perlite as the cutting medium, put it into the plug tray and disinfect it; (4) soaking the cuttings obtained in step (2) in a rooting agent and inserting them into a plug tray matrix, watering them thoroughly with a sterilizing solution, and covering them with a transparent cover; (5) After cutting, place under 70% shading conditions. After the new leaves are unfolded, adjust the shading to 50% and the temperature to 10℃~30℃. Rehydrate in time after cutting and sterilize with bactericidal liquid. Remove the transparent cover gradually after 45 days of cutting and water as needed; (6) Transplant and plant 60 days after cutting.

2. The hard branch cutting propagation method of Deutzia glabra according to claim 1, characterized in that: The rooting agent is one or both of NAA and IBA.

3. The hard branch cutting propagation method of Deutzia glabra according to claim 2, characterized in that: The NAA concentration is 1000 mg / L.

4. The hard branch cutting propagation method of Deutzia glabra according to claim 1, characterized in that: In step (5), the method of cutting tubes is: open the cover every 3 days to check the rooting situation and add water once, and apply sterilization liquid every 15 days in combination with watering.

5. The hard branch cutting propagation method of Deutzia glabra according to claim 1, characterized in that: In step (3), the mass ratio of peat to perlite is 4:

1.

6. The hard branch cutting propagation method of Deutzia glabra according to claim 1, characterized in that: The rooting agent treatment method is: soak 3cm from the base of the cuttings and treat for 1 minute.

7. The hard branch cutting propagation method of Deutzia glabra according to claim 1, characterized in that: The sterilization solution is 1000 times diluted 50% carbendazim wettable powder.

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