Cultivation and planting method for improving quality of gastrodia elata

Abstract

The invention provides a cultivation and planting method for improving the quality of gastrodia elata, and relates to the technical field of gastrodia elata planting. In the cultivation process of the armillaria mellea strain, the armillaria mellea strain is cultivated by preparing different culture media in a specific stage, the obtained armillaria mellea strain is good in state, and the number of hypha cultivation days is small. Moreover, the gastrodia elata is piled and cultured in a #-shaped placing manner, so that the yield of the gastrodia elata can be remarkably increased, and meanwhile, the contents of gastrodin and gastrodin in the gastrodia elata are increased. Coumarin-3-carboxylic acid is added into the slant culture medium in the cultivation stage of armillaria mellea, and the rattan orange is added into the three-stage culture medium, so that the pH value of the culture medium can be adjusted on one hand, the growth and development of gastrodia elata are facilitated on the other hand, and the content of active ingredients can be indirectly increased possibly.

Description

Technical Field

[0001] The invention relates to the technical field of gastrodia elata planting, and in particular to a cultivation method for improving gastrodia elata quality. Background Art

[0002] Gastrodia elata is a precious Chinese herbal medicine that can be used as both medicine and food. It is in huge demand, and its underground tubers are an important source of active substances. However, the current Gastrodia elata planting technology is relatively traditional, with problems such as low yield and uneven quality. Ingredients such as gastrodin and gastrodin aglycone in Gastrodia elata have significant health benefits. However, the content of active ingredients in Gastrodia elata is affected by many factors, including planting environment, variety, cultivation technology and management measures. The current common planting technology is difficult to effectively regulate the physiological process of Gastrodia elata, resulting in limited production of its active ingredients, affecting the quality and market competitiveness of Gastrodia elata medicinal materials. In addition, Gastrodia elata has a long growth cycle and is susceptible to pests and diseases. In addition, the traditional planting method lacks scientific guidance and cannot fully meet the market demand for high-quality Gastrodia elata. Summary of the invention

[0003] To achieve the above objectives, the present invention is implemented through the following technical solutions: The present invention proposes a cultivation method for improving the quality of Gastrodia elata, comprising the following steps: S1. Cultivation of primary Ampelopsis strains: inoculating Ampelopsis hyphae into a slant culture medium and culturing in the dark at 20-25° C. for 12-15 days to obtain primary strains; the contents of the components in the slant culture medium are: 150-300 g / L potato, 10-20 g / L glucose, 2-5 g / L potassium dihydrogen phosphate, 20-50 mg / L coumarin-3-carboxylic acid, and 25-30 g / L agar; S2. Cultivation of secondary Ameloblasts strains: inoculating the primary strains into the secondary culture medium and culturing at 24-26° C. for 10-15 days to obtain secondary strains; S3: Cultivation of Armillaria strains: inoculating the secondary strains into the tertiary culture medium at 25-27° C. and culturing for 30-35 days to obtain Armillaria strains; the contents of the components in the tertiary culture medium are: 50-80 g / L sawdust, 30-50 g / L broadleaved tree twigs, 30-50 g / L citronella, 1-5 g / L quicklime, 2-5 g / L sucrose and 1-5 g / L potassium dihydrogen phosphate; S4. Planting of Gastrodia elata: Arrange basswood in two layers in a tic-tac-toe shape, and inoculate each basswood layer with Gastrodia elata and Armillaria fasciata species for planting.

[0004] Further, inoculating the mycelium of Armillaria mellifera in a slant culture medium and culturing in the dark at 20-25° C. for 12-15 days to obtain a primary strain comprises the following steps: Cut and crush the potatoes, add 500-700 mL of water, heat and dissolve them, and obtain a mixture; The obtained mixture is mixed with coumarin-3-carboxylic acid, glucose and agar, and after mixing, 1000 mL of water is added and the mixture is heated and dissolved until the pH is 5.0-6.0, and the mixture is filtered while hot and sterilized with high pressure steam to obtain a slant culture medium; The mycelium of Armillaria flexneri is inoculated on the slant culture medium at an inoculation amount of 2-5% of the volume of the slant culture medium, and cultured in the dark at 20-25° C. for 12-15 days to obtain a primary strain.

[0005] Furthermore, the contents of the components in the secondary culture medium are: glucose 20-50 g / L, potassium dihydrogen phosphate 1-3 g / L, magnesium sulfate 2-5 g / L, vitamin B1 2-5 g / L and wheat bran 30-40 g / L.

[0006] Further, the primary strain is inoculated into the secondary culture medium and cultured at 24-26° C. for 10-15 days to obtain the secondary strain; comprising the following steps: After the wheat bran is crushed and sterilized, 10 times the volume of deionized water is added for soaking; Glucose, potassium dihydrogen phosphate, magnesium sulfate, vitamin B1 and soaked wheat bran are sequentially added into water and stirred, and the pH value is adjusted to 5.0-6.0 and sterilized by high pressure steam to obtain a secondary culture medium; The primary strain is inoculated on the secondary culture medium at an inoculation amount of 5-10% of the volume of the secondary culture medium, and cultured at 24-26° C. for 10-13 days to obtain the secondary strain.

[0007] Further, inoculating the secondary bacterial strain into the tertiary culture medium and culturing at 25-27° C. for 30-35 days to obtain the Amphiprion strain comprises the following steps: The vine orange is dried and then crushed, ethanol is added to heat and dissolve, sawdust and broad-leaved tree twigs are exposed to the sun, crushed and mixed with the dissolved vine orange, and sterilized with high-pressure steam to obtain a mixture; Mixing quicklime, sucrose and potassium dihydrogen phosphate at room temperature to obtain mixed material 2; Adding deionized water to the mixture 1 and the mixture 2 to mix, thereby obtaining a pretreatment culture medium having a water content of 50-70%; The pre-treated culture medium is placed in a plastic bag to prepare a bacterial bag, which is then stuffed for 5 to 10 hours and sterilized by high pressure steam to obtain a tertiary culture medium; After sterilization, the secondary strain is inoculated into the tertiary culture medium at an inoculation amount of 10-12% of the volume of the tertiary culture medium, and cultured at 25-27° C. for 30-35 days to obtain bagged Ameloblastoma strains.

[0008] Furthermore, the sterilization conditions are 120-122° C. and 15-30 minutes.

[0009] Furthermore, arranging basswood in two layers in a tic-tac-toe pattern, and inoculating hemp seeds and cordaria species on each basswood layer for planting includes the following steps: Place a layer of leaves at the bottom of the experimental pit, and then place basswood with a diameter of 12-20 cm closely in the experimental pit to form the first layer of basswood; After placing the first layer of basswood, place 6-7 pieces of hemp seeds and cordaria species on both sides of each basswood, at its fish scale mouth and at the end of the basswood. After watering, sprinkle dead branches and leaves or rice husks on the inoculated first layer of basswood as fillers, spread them flat and cover them with soil. Place the second layer of basswood at right angles to the first layer at intervals of 1-3cm. Place 4-5 pieces of hemp seeds and cordaria species at the end and scales of each basswood to form the second layer of basswood. Sprinkle a small amount of dead branches and leaves or rice husk crumbs as filler, flatten them and cover them with soil.

[0010] Furthermore, the diameter of the first layer of basswood and the second layer of basswood are both 10-15 cm, and the length is both 50-70 cm.

[0011] Furthermore, the types of basswood include one or more of Cyclobalanopsis glauca, Chestnut tree, Oak tree, Oak tree, and Quercus variabilis.

[0012] Furthermore, during the planting process, the leaves of Gastrodia elata are sprayed with water every 7-15 days.

[0013] The present invention has the following advantages: In the process of cultivating the Armillaria species, the present invention cultivates the Armillaria species by configuring different culture media at specific stages, so that the obtained Armillaria species are in a better state and the number of days for mycelium cultivation is shorter. In addition, the Armillaria species are used to pile up Gastrodia elata in a tic-tac-toe arrangement manner, which can significantly increase the yield of Gastrodia elata and improve the contents of gastrodin and gastrodin in Gastrodia elata. In the cultivation stage of Armillaria, the present invention adds coumarin-3-carboxylic acid to the slant culture medium and adds citronella to the tertiary culture medium, which can adjust the pH value of the culture medium on the one hand, and is also beneficial to the growth and development of Gastrodia elata on the other hand, and indirectly improves the content of active ingredients. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0015] Figure 1It is a statistical diagram of the number of flower buds in each experimental group and control group during the cultivation process of Gastrodia elata in the embodiment of the present invention; Figure 2 Shown is a picture of Gastrodia elata harvested from Experimental Group 1 of the embodiment of the present invention; Figure 3 The statistical graph of the contents of gastrodin and gastrodin aglycone in each experimental group and control group in the embodiments of the present invention is shown. DETAILED DESCRIPTION

[0016] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention is clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0017] Example 1 1.1 Cultivation of primary strains; Prepare the slant culture medium according to the culture medium components in Table 1: Table 1

[0018] Prepare slant culture medium according to the above formula and inoculate the mycelium of Ampelopsis pilosula to obtain the primary strain. The specific process is as follows: (1) Weigh 200 g of potatoes, cut and crush them, add 500 mL of water, heat and dissolve them to obtain a mixture; (2) The obtained mixture was mixed with 30 mg of coumarin-3-carboxylic acid (when preparing medium A2, coumarin-3-carboxylic acid was not added), 20 g of glucose, 3 g of potassium dihydrogen phosphate, and 25 g of agar, and then the mixture was made up to 1 L with water and continued to be heated until the pH value was 5.0. The mixture was filtered while hot and sterilized with high pressure steam (121°C, 20 minutes) to obtain a slant culture medium; (3) Inoculate the hyphae of Gallia gallica on the slant culture medium at an inoculum amount of 3% of the volume of the slant culture medium used, and culture in the dark at 22°C for 13 days to obtain a primary strain.

[0019] 1.2 Cultivation of secondary strains; Prepare the secondary culture medium according to the culture medium components in Table 2: Table 2

[0020] (1) 35 g of wheat bran was crushed and sterilized, and then added into 10 times the volume of deionized water for soaking to obtain soaked wheat bran; If vine orange is added, 30 g of vine orange needs to be dried and then crushed, added with ethanol to heat and dissolve, and then mixed with the components in step (2). The remaining subsequent steps are the same.

[0021] If coumarin-3-carboxylic acid is added, coumarin-3-carboxylic acid is directly combined with the components in step (2), and the remaining subsequent steps are the same.

[0022] (2) 20 g of glucose, 2 g of potassium dihydrogen phosphate, 2 g of magnesium sulfate (magnesium sulfate heptahydrate), 2 g of vitamin B1 and soaked wheat bran are added to 1 L of water in sequence and stirred. The pH value is adjusted to 5.5 and then sterilized with high-pressure steam (121°C, 20 minutes) to obtain a secondary culture medium; (3) Inoculate the primary strain into the secondary culture medium at a volume of 10% of the volume of the primary culture medium and culture at 24°C for 11 days to obtain the secondary strain.

[0023] 1.3 Cultivation of Ameloblastoma strains; Prepare the tertiary culture medium according to the culture medium components in Table 3: Table 3

[0024] (1) 30 g of vine fruit was dried and crushed, and ethanol was added to heat and dissolve (if there is no vine fruit in the culture medium, this operation is omitted and it is not mixed with vine fruit in the subsequent process), and then sawdust and broad-leaved tree twigs were exposed to the sun, crushed, and mixed with the dissolved vine fruit, and sterilized by high-pressure steam to obtain a mixture 1; (2) 1 g of quicklime, 5 g of sucrose and 1 g of potassium dihydrogen phosphate are mixed at room temperature to obtain a mixture 2; (3) adding deionized water to the mixture 1 and the mixture 2 to mix, thereby obtaining a pretreatment medium having a water content of 50-70%; (4) The pre-treated culture medium is placed in a plastic bag to prepare a culture bag, which is then stuffed for 7 hours and sterilized by high pressure steam (121° C., 20 minutes) to obtain a tertiary culture medium; (5) After sterilization, the secondary bacterial strain is inoculated into the tertiary culture medium at an inoculum amount of 10% of the volume of the culture medium and cultured at 26°C for 30 days to obtain bagged Ringerella strains.

[0025] Referring to the above preparation method, different culture media were selected at different culture stages of Amphiprion, different culture media combinations (combination 1-combination 6) were set, the mycelial growth was observed regularly, and the number of days it took for the mycelium to fill the spawn bag (the time from the beginning of the growth of Amphiprion mycelium to the filling of the spawn bag on the tertiary culture medium) was recorded. The results are shown in Table 4: Table 4

[0026] It can be seen from Table 4 that when the combination of culture media is combination 1 (ie, A1+B1+C1), the growth state of mycelium and the number of days of mycelium growth are the shortest and the mycelium state is the best.

[0027] Example 2 The planting time of Gastrodia elata is from March to April, and the harvest time is from December of the current year to January of the following year. The specific planting method is as follows: Different experimental groups were set up using the Armillaria strains cultured in Example 1 (the Armillaria strains of combinations 1-6 were set up in experimental groups 1-6 respectively (two parallel experimental groups were set up for each Armillaria strain combination)) to plant Gastrodia elata. The specific planting process is as follows: A layer of leaves 1-2 cm thick was laid on the bottom of the experimental pit, and then basswood with a diameter of 12-15 cm was placed closely in the experimental pit to form the first layer of basswood; After placing the first layer of basswood, place 6-7 pieces of hemp seeds and cordaria species on both sides of each basswood, at its fish scale mouth and at the end of the basswood. After watering, sprinkle a small amount of dead branches and leaves or rice husk powder on the inoculated first layer of basswood as filler to level it. Place the second layer of basswood in a direction perpendicular to the first layer (forming a "well" shape with the first layer of basswood) at intervals of 1-3cm. Place 4-5 pieces of hemp seeds and cordaria species at the end of each basswood and the fish scales on both sides to form the second layer of basswood. Sprinkle a small amount of dead branches and leaves or rice husk crumbs as filler, flatten it, and cover it with soil.

[0028] Comparative Example 1 Gastrodia elata was planted according to the method of Example 2 (using the Armillaria fasciata in Experimental Group 1 for planting), except that the second layer of basswood was placed in the same direction as the first layer of basswood (as Control Group 1).

[0029] Test Example 1 The growth process of Gastrodia elata planted according to the methods in Example 2 and Comparative Example 1 was observed.

[0030] After observing the flower buds during the flowering process of Gastrodia elata, it was found that the flower buds of Gastrodia elata planted with the Cordyceps sinensis in experimental group 1 were firm and compact, without yellowing or looseness, and the inflorescence was complete, without deformity or damage. The number of flowers was greater than that of the Cordyceps sinensis species in experimental groups 2-6. The specific data are as follows: Figure 1 As shown (the data in the figure are average values), it can be seen that the number of flower buds in experimental group 1 is significantly higher than that in experimental groups 2-6 and the control group.

[0031] When harvesting, the Gastrodia elata in each experimental group was dug up and its appearance was observed. It can be seen that the surface of the Gastrodia elata in the experimental groups 1-6 and the control group 1 has a uniform color, clear surface texture, and no cracks or damage (such as Figure 2 The Gastrodia elata in each experimental group and control group were weighed respectively, and the number of Gastrodia elata weighing more than 1 kg harvested in each square meter of planting hole was counted. The results are shown in Table 5: Table 5

[0032] As can be seen from Table 5, the individual weight, quantity and overall weight of Gastrodia elata in experimental group 1 were better than those in experimental groups 2-5 and control group 1.

[0033] Test Example 2 Detection of active ingredients in Gastrodia elata: HPLC-MS was used to determine the active ingredients gastrodin and gastrodin aglycone in Gastrodia elata. The specific process is as follows: Preparation of test solution: After drying the Gastrodia elata grown according to the above-mentioned different methods, samples were taken from the head and tail and the middle part of the Gastrodia elata respectively. After the Gastrodia elata samples obtained from different parts were crushed, about 5g of powder was taken and passed through a No. 3 sieve, 75mL of ethanol solution was added, shaken and allowed to stand for 2h, the mass was weighed, heated to reflux for 2.5h, the solution was cooled and the mass was weighed again, the insufficient part was supplemented with ethanol solution, shaken and filtered, 10mL of the filtrate was accurately drawn with a pipette, and steamed in a water bath until nearly dry, the residue was dissolved with a mobile phase solution and transferred to a 25mL volumetric flask, the mobile phase solution was diluted to the scale and shaken, filtered, and the filtrate was taken to obtain the test solution for content test (the test result was obtained by taking the average of the test results of three Gastrodia elata in the same acupoint).

[0034] Preparation of reference solution: accurately weigh gastrodin and gastrodin aglycone reference substances, add mobile phase to dissolve and dilute to appropriate concentration. After accurately weighing the reference substance, dilute the mobile phase solution to prepare a mixed reference solution containing 0.302 mg / mL and 0.0996 mg / mL of gastrodin, store in a -20℃ refrigerator for later use.

[0035] The measurement conditions are as follows: Chromatographic conditions: The chromatographic column was a Hypersil C18 column (250 mm × 4.6 mm, 2.5 μm), the mobile phase was a 0.1% formic acid aqueous buffer solution: methanol solution for gradient elution, and the analysis time was 80 min. The electrospray ion source was used for negative ion mode monitoring, the source spray voltage was -4500 V, and the ion source temperature was 550 °C.

[0036] Mass spectrometry conditions: negative ion mode monitoring with electrospray ion source, source spray voltage of -4500V, ion source temperature of 550°C, heating at the interface, nitrogen gas introduced throughout, multiple reaction monitoring mode (MRM) for quantitative analysis. Nebulizer gas (N2) pressure was 55psi, auxiliary gas (N2) pressure was 55psi, and curtain gas (N2) pressure was 35psi.

[0037] The contents of gastrodin and gastrodin in the test solution were determined according to the above determination conditions. The results are as follows: Figure 3 As shown, it can be seen that the contents of gastrodin and gastrodin in the Gastrodia elata cultivated by the experimental group 1 and according to the method of Example 1 are 0.36% and 0.23%, respectively, which are much higher than the 0.20% specified in the 2005 edition of the Chinese Pharmacopoeia (Volume 1), and higher than the requirement of the Pharmacopoeia that the contents of gastrodin and gastrodin in dried Gastrodia elata should not be less than 0.25%.

[0038] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A cultivation and planting method for improving the quality of Gastrodia elata, characterized in that: The following steps are involved: S1. Cultivation of primary Ampelopsis strains: inoculating Ampelopsis hyphae into a slant culture medium and culturing in the dark at 20-25° C. for 12-15 days to obtain primary strains; the contents of the components in the slant culture medium are: 150-300 g / L potato, 10-20 g / L glucose, 2-5 g / L potassium dihydrogen phosphate, 20-50 mg / L coumarin-3-carboxylic acid, and 25-30 g / L agar; S2. Cultivation of secondary Ameloblasts strains: inoculating the primary strains into the secondary culture medium and culturing at 24-26° C. for 10-15 days to obtain secondary strains; S3: Cultivation of Armillaria strains: inoculating the secondary strains into the tertiary culture medium at 25-27° C. and culturing for 30-35 days to obtain Armillaria strains; the contents of the components in the tertiary culture medium are: 50-80 g / L sawdust, 30-50 g / L broadleaved tree twigs, 30-50 g / L citronella, 1-5 g / L quicklime, 2-5 g / L sucrose and 1-5 g / L potassium dihydrogen phosphate; S4. Planting of Gastrodia elata: Arrange basswood in two layers in a tic-tac-toe shape, and inoculate each basswood layer with Gastrodia elata and Armillaria fasciata species for planting.

2. The method for cultivating and planting gastrodia elata for improving its quality according to claim 1, characterized in that: The mycelium of Armillaria mellifera is inoculated into a slant culture medium and cultured in the dark at 20-25° C. for 12-15 days to obtain a primary strain, which includes the following steps: Cut and crush the potatoes, add 500-700 mL of water, heat and dissolve them, and obtain a mixture; The obtained mixture is mixed with coumarin-3-carboxylic acid, glucose and agar, and after mixing, 1000 mL of water is added and the mixture is heated and dissolved until the pH is 5.0-6.0, and the mixture is filtered while hot and sterilized with high pressure steam to obtain a slant culture medium; The mycelium of Armillaria flexneri is inoculated on the slant culture medium at an inoculation amount of 2-5% of the volume of the slant culture medium, and cultured in the dark at 20-25° C. for 12-15 days to obtain a primary strain.

3. The method for cultivating and planting gastrodia elata for improving its quality according to claim 1, characterized in that: The contents of the components in the secondary culture medium are: 20-50 g / L of glucose, 1-3 g / L of potassium dihydrogen phosphate, 2-5 g / L of magnesium sulfate, 2-5 g / L of vitamin B1 and 30-40 g / L of wheat bran.

4. The method for cultivating and planting gastrodia elata for improving its quality according to claim 3, characterized in that: The primary strain is inoculated into the secondary culture medium and cultured at 24-26° C. for 10-15 days to obtain the secondary strain; the steps include: After the wheat bran is crushed and sterilized, 10 times the volume of deionized water is added for soaking; Glucose, potassium dihydrogen phosphate, magnesium sulfate, vitamin B1 and soaked wheat bran are sequentially added into water and stirred, and the pH value is adjusted to 5.0-6.0 and sterilized by high pressure steam to obtain a secondary culture medium; The primary strain is inoculated on the secondary culture medium at an inoculation amount of 5-10% of the volume of the secondary culture medium, and cultured at 24-26° C. for 10-13 days to obtain the secondary strain.

5. The method for cultivating and planting gastrodia elata for improving its quality according to claim 1, characterized in that: The secondary bacterial strain is inoculated into the tertiary culture medium and cultured at 25-27° C. for 30-35 days to obtain the Amphiprion strain, which comprises the following steps: The vine orange is dried and then crushed, ethanol is added to heat and dissolve, sawdust and broad-leaved tree twigs are exposed to the sun, crushed and mixed with the dissolved vine orange, and sterilized with high-pressure steam to obtain a mixture; Mixing quicklime, sucrose and potassium dihydrogen phosphate at room temperature to obtain mixed material 2; Adding deionized water to the mixture 1 and the mixture 2 to mix, thereby obtaining a pretreatment culture medium having a water content of 50-70%; The pre-treated culture medium is placed in a plastic bag to prepare a bacterial bag, which is then stuffed for 5 to 10 hours and sterilized by high pressure steam to obtain a tertiary culture medium; After sterilization, the secondary strain is inoculated into the tertiary culture medium at an inoculation amount of 10-12% of the volume of the tertiary culture medium, and cultured at 25-27° C. for 30-35 days to obtain bagged Ameloblastoma strains.

6. The method for cultivating and planting gastrodia elata for improving its quality according to claim 2, 4 or 5, characterized in that: The sterilization conditions are 120-122°C and 15-30 minutes.

7. The method for cultivating and planting gastrodia elata for improving its quality according to claim 1, characterized in that: The following steps are involved in arranging two layers of basswood in a tic-tac-toe pattern and inoculating hemp seeds and cordaria species on each basswood layer: Place a layer of leaves at the bottom of the experimental pit, and then place basswood with a diameter of 12-20 cm closely in the experimental pit to form the first layer of basswood; After placing the first layer of basswood, place 6-7 pieces of hemp seeds and cordaria species on both sides of each basswood, at its fish scale mouth and at the end of the basswood. After watering, sprinkle dead branches and leaves or rice husks on the inoculated first layer of basswood as fillers, spread them flat and cover them with soil. Place the second layer of basswood at right angles to the first layer at intervals of 1-3cm. Place 4-5 pieces of hemp seeds and cordaria species at the end and scales of each basswood to form the second layer of basswood. Sprinkle a small amount of dead branches and leaves or rice husk crumbs as filler, flatten them and cover them with soil.

8. The method for cultivating and planting gastrodia elata for improving its quality according to claim 7, characterized in that: The diameters of the first layer of basswood and the second layer of basswood are both 10-15 cm and the lengths are both 50-70 cm.

9. The method for cultivating and planting Gastrodia elata for improving its quality according to claim 7, characterized in that: The types of basswood include one or more of Cyclobalanopsis glauca, Chestnut tree, Oak tree, Oak tree, and Quercus variabilis.

10. The method for cultivating and planting Gastrodia elata for improving its quality according to claim 1, characterized in that: During the planting process, spray water on the leaves of Gastrodia elata every 7-15 days.

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