Fresh-keeping liquid for shaping cut carnation flower with flower mud and application of fresh-keeping liquid

A preservative solution with 8-hydroxyquinoline citrate, benzalkonium chloride, nano-silver, citric acid, salicylic acid, and sucrose addresses microbial and ethylene issues in carnation cut flowers, enhancing their preservation and longevity in floral mud models.

CN119214152APending Publication Date: 2024-12-31SUZHOU XIANLONG BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202410874995.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Priority Date
2023-06-30
Filing Date
2024-07-01
Publication Date
2024-12-31

AI Technical Summary

Technical Problem

Carnation cut flowers in floral mud modeling face issues with microbial growth, water absorption difficulty, and ethylene sensitivity, leading to stem blockage, premature bending, and reduced vase life, while existing preservatives are toxic or ineffective.

Method used

A preservative solution comprising 8-hydroxyquinoline citrate, benzalkonium chloride, nano-silver, citric acid, salicylic acid, alkyl polyglycoside, and sucrose, which inhibits bacterial growth, ethylene production, and promotes flower opening and longevity.

Benefits of technology

The solution effectively inhibits bacterial growth, reduces mildew, improves flower arrangement quality, delays aging, and prolongs the ornamental period of carnations by 32.8% to 45.7%, with reduced toxicity and ease of use.

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Abstract

The invention relates to a fresh-keeping solution for shaping of cut flowers of carnation and application of the fresh-keeping solution in shaping of cut flowers of carnation, the fresh-keeping solution comprises a bactericide, an organic acid, a plant growth regulator, cane sugar, a surfactant and water, and the bactericide is selected from one or more of 8-hydroxyquinoline citrate, benzalkonium chloride and nano-silver; the organic acid is citric acid, the plant growth regulator is salicylic acid, and the surfactant is alkyl polyglycoside. The invention also provides an application of the fresh-keeping liquid in the fresh keeping of the cut flower in the shape of the mud carnation cut flower. The preservative can promote the cut dianthus caryophyllus flowers to bloom, inhibit aging and prolong the service life of the cut dianthus caryophyllus flowers.
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Description

Technical Field

[0001] The invention relates to the technical field of cut flower preservation, and in particular to a preservative liquid used for floral mud carnation cut flower shaping and application thereof in floral mud carnation cut flower shaping. Background Art

[0002] Dianthus caryophyllus is a perennial herbaceous flower of the genus Dianthus in the family Caryophyllaceae. As one of the four major cut flowers, it has the advantages of a variety of flower colors and a long flowering period.

[0003] Carnations are often suitable for making various shapes and patterns in flower arrangement, which is usually achieved by using floral mud as a fixing material. This type of application of carnations is collectively referred to as floral mud carnation cut flower shapes.

[0004] Flower mud is mostly green, and is usually a sponge-like foam made of phenolic resin. Flower mud is very light, can be cut at will, and has strong water absorption. After absorbing water, the flower mud can support flower branches for shaping. Flower mud itself does not contain ingredients that are beneficial to cut flowers, and cannot provide the nutrients needed for cut flowers. Therefore, you can use cut flower preservative liquid to soak the flower mud, which helps to improve the quality of carnation cut flowers inserted in the flower mud and extend its cut flower life.

[0005] Flower mud materials usually do not have any material that damages carnation cut flowers, but flower mud itself has abundant pores, which provide a favorable place for the growth of microorganisms. In addition, flower mud has strong water absorption, so it is not easy for cut flowers to compete and absorb enough preservatives (or nutrient solutions) from flower mud. In short, flower mud cut flower modeling and vase insertion provide completely different microenvironments for cut flowers, so the existing carnation-specific vase insertion liquid cannot provide effective preservation for flower mud carnation cut flower modeling.

[0006] Moreover, microorganisms, mainly bacteria, are prone to breed and multiply at the cut of carnations, causing stem blockage and water absorption obstruction. When the temperature of carnation cut flowers is around 25℃, if the water in the bottle is not changed for one day, there will be a peculiar smell, and if the water is not changed for three days, the flower stems will rot in the water. In addition, the stems are prone to premature bending.

[0007] In addition, carnation is an ethylene-sensitive cut flower. Ethylene inhibitors silver nitrate and silver thiosulfate have a significant preservative effect on carnation, but they are highly toxic and pollute the environment, so they are not recommended for use in carnation cut flower mud preservative liquid.

[0008] Therefore, in view of the problems of microbial growth and water absorption difficulty faced by carnation cut flowers in flower mud cut flower modeling, it is very necessary to provide a preservative liquid that can effectively preserve the carnation cut flowers for the flower mud carnation cut flower modeling.

[0009] In addition, on the one hand, there are differences in understanding among those skilled in the art; on the other hand, the applicant studied a large number of documents and patents when making the present invention, but due to space limitations, not all details and contents are listed in detail. However, this does not mean that the present invention does not have the characteristics of these prior arts. On the contrary, the present invention already has all the characteristics of the prior art, and the applicant reserves the right to add relevant prior art to the background technology. Summary of the invention

[0010] In view of the shortcomings of the prior art, the first aspect of the present invention provides a carnation cut flower preservative solution, which comprises a bactericide, an organic acid, a plant growth regulator, sucrose, a surfactant and water. Preferably, the preservative solution consists of a bactericide, an organic acid, a plant growth regulator, sucrose, a surfactant and water.

[0011] According to some preferred embodiments, the bactericide includes at least one or more of 8-hydroxyquinoline citrate, benzalkonium chloride and nano-silver, preferably at least two, and more preferably a combination of 8-hydroxyquinoline citrate, benzalkonium chloride and nano-silver. There are countless known bactericides, and there are tens of thousands of bactericides used for cut flower preservation, but different cut flowers or different preservation application environments require appropriate bactericides or bactericide combinations to achieve effective sterilization. The inventors have found that for the application of flower mud carnation cut flower modeling, 8-hydroxyquinoline citrate, benzalkonium chloride and nano-silver have very excellent effects, especially when all three are used.

[0012] According to some preferred embodiments, the organic acid is citric acid.

[0013] According to some preferred embodiments, the plant growth regulator is salicylic acid.

[0014] According to some preferred embodiments, the surfactant is an alkyl polyglycoside.

[0015] According to some preferred embodiments, the sucrose is used as an energy source.

[0016] According to some preferred embodiments, the carnation cut flower preservative solution includes 100-200 mg / L 8-hydroxyquinoline citrate, 10-50 mg / L benzalkonium chloride, 5-50 mg / L nanosilver, 100-500 mg / L citric acid, 10-40 mg / L salicylic acid, 10-40 μl / L alkyl polyglycosides and 10-30 g / L sucrose.

[0017] According to some preferred embodiments, the carnation cut flower preservative solution includes 100-200 mg / L 8-hydroxyquinoline citrate, 10-50 mg / L benzalkonium chloride, 5-50 mg / L nanosilver, 100-500 mg / L citric acid, 10-40 mg / L salicylic acid, 10-40 μl / L alkyl polyglycosides, 10-30 g / L sucrose and the remainder water.

[0018] Salicylic acid has the effect of inhibiting ethylene synthesis and prolonging the life of cut flowers. Studies have found that salicylic acid can increase the fresh weight of carnation cut flowers, increase flower diameter, and improve ornamental quality.

[0019] Nanosilver is a new type of antibacterial material with high antibacterial activity, broad antibacterial spectrum and low toxicity. The preservation solution containing nanosilver and sucrose can extend the vase life of carnation. Studies have shown that nanosilver can react with hydrogen peroxide in cells to release Ag+. This indicates that in addition to antibacterial ability, nanosilver may also have an ethylene antagonistic effect.

[0020] According to some preferred embodiments, nano silver and salicylic acid have the effect of inhibiting ethylene. The total amount of nano silver and salicylic acid should not exceed 50 mg / L.

[0021] The method for using the fresh-keeping liquid of the present invention may include:

[0022] (1) Prepare the preservative solution: Dissolve salicylic acid of the desired concentration with an appropriate amount of alcohol, add 8-hydroxyquinoline citrate, benzalkonium chloride, nanosilver, citric acid and sucrose to tap water according to the desired concentration under stirring, and finally add alkyl polyglycoside and salicylic acid dissolved in alcohol, and stir until uniform. Pour the preservative solution into a container, pour the preservative solution with the water level higher than the flower mud, soak the flower mud in the preservative solution, and let the flower mud slowly sink into the liquid and absorb the liquid.

[0023] Trim the carnation stems to a length of 7 cm (expressed here as about 7 cm, for example 5 to 10 cm, such as 5, 6, 7, 8, 9 or 10 cm, or some subrange therebetween, not necessarily exactly 7 cm), and insert them into the floral mud soaked with preservative liquid to a depth of about 2 to 3 cm.

[0024] According to some preferred embodiments, the fresh-keeping solution comprises 200 mg / L 8-hydroxyquinoline citrate, 50 mg / L benzalkonium chloride, 10 mg / L nanosilver, 500 mg / L citric acid, 40 mg / L salicylic acid, 30 μl / L alkyl polyglycosides and 10 g / L sucrose.

[0025] According to some preferred embodiments, the fresh-keeping solution comprises 200 mg / L 8-hydroxyquinoline citrate, 50 mg / L benzalkonium chloride, 10 mg / L nanosilver, 500 mg / L citric acid, 40 mg / L salicylic acid, 30 μl / L alkyl polyglycosides, 10 g / L sucrose and the balance water.

[0026] Compared with the prior art, the advantages of the present invention include:

[0027] (1) When the carnation cut flower preservative liquid formula of the present invention is used to preserve carnation, it can inhibit bacterial growth, reduce mildew of flower mud, promote opening, and improve the flower arrangement quality of carnation.

[0028] (2) The carnation cut flower preservative liquid of the present invention can inhibit the generation of ethylene.

[0029] (3) The carnation cut flower preservative liquid of the present invention can delay the aging and wilting of flowers and prolong the ornamental period of carnation;

[0030] (4) The carnation cut flower preservative liquid of the present invention has low cost and is easy to operate. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 Changes in the amounts of ethylene produced in Comparative Example 1 and Example 4 are shown.

[0032] Figure 2 A photograph showing the shape of cut carnation flowers in flower mud treated with freshness in Comparative Example 1.

[0033] Figure 3 A photograph showing the shape of cut carnation flowers in flower mud treated for freshness in Example 1 is shown.

[0034] Figure 4 A photograph showing the shape of cut carnation flowers in flower mud treated for freshness in Example 2.

[0035] Figure 5 A photograph showing the shape of cut carnation flowers in flower mud treated for freshness in Example 3.

[0036] Figure 6 A photograph showing the shape of cut carnation flowers in flower mud treated with freshness-preservation in Example 4. DETAILED DESCRIPTION

[0037] The present invention will be further described below by way of examples, but these examples are only for illustrative purposes and should not be construed as limiting the scope of protection of the present invention.

[0038] The formula concentrations of the preservative solutions of Comparative Example 1 and Examples 1-4 are shown in Table 1. All components are commercially available, among which alkyl polyglycosides are purchased from Jiangxi Jinfanda Biochemical Co., Ltd. (alkyl polyglycosides 0810), and other components are also conventional components.

[0039] Table 1

[0040]

[0041] The experimental flowers were red carnation cut flowers purchased from the flower market, and the cut flowers had strong stems and uniform bud sizes.

[0042] Place the flower mud in the preservative solution of each embodiment and comparative example, and allow it to absorb naturally until saturated. Trim the carnation stems to a length of 7 cm and insert them into the wet flower mud to a depth of about 3 cm. Repeat 3 times for each embodiment, and use 5 carnations for each repeat. After inserting, place the flower mud indoors for observation. The ambient temperature is controlled to 22°C ± 2°C, and the relative humidity is 60-70%. Replenish the liquid from just above the flower mud every 2 days, about 150 mL each time.

[0043] Observe the opening and wilting of carnation cut flowers every three days until they lose their ornamental value. Record the life span of the cut flowers. The standard for the end of the life span of carnation cut flowers is that the petals wilt, curl, fade, and dry up.

[0044] Flower diameter increase rate (%): (flower diameter on the nth day - flower diameter on the day of treatment) / flower diameter on the day of treatment * 100%.

[0045] Flower wilting rate (%): The percentage of flowers with wilted, curled, faded petals among the total number of flowers.

[0046] Ethylene production: Measure the ethylene production on the 5th, 7th and 9th day after vase insertion. The unit is n1 / (gFW*h).

[0047] Vase life (d): When 50% or more of the flowers begin to wilt, curl, fade, dry up, and lose their ornamental value, the vase life of the cut flower is considered to have ended, i.e. the number of days from the date of vase placement to the end of the cut flower vase placement period.

[0048] The measured index value of each treatment group is the average measured value of the cut flowers in that treatment group.

[0049] The results are shown in Table 2 and Figures 1 to 6 shown.

[0050] The life span of the carnation cut flowers in Examples 1-4 was significantly prolonged. Compared with 11.6 days in the control example, the life span of the carnations in the Examples was greater than 15 days, and the life span of the carnations in Examples 1-4 in the flower mud was prolonged by 32.8%, 39.7%, 42.2%, and 45.7%, respectively. This indicates that the carnation cut flower preservative liquid in the invention can prolong the ornamental life span of the carnations in the flower mud.

[0051] From 0 to 9 days after treatment, the flower diameter increase rate of the carnation cut flowers in the control group and each embodiment showed an upward trend, and the flower diameter reached a maximum value at about 9 days, and then slightly decreased. The carnation flower diameter of Example 4 increased the most, increasing by 102.3%, while the maximum flower diameter increase rate of the control group was 87.9%.

[0052] On the 5th, 7th and 9th days after treatment, the ethylene production of the control group and each example was as follows: Figure 1 As shown. The ethylene production of the control group was higher on the 7th day than on the 5th and 9th day, with a small peak. The ethylene production of each example was not much different, and the ethylene production of Examples 2 and 4 was slightly lower than that of Examples 1 and 3. On the 5th, 7th, and 9th days, there was an upward trend, all lower than the control group (see Figure 1 ).

[0053] Eight days after treatment, the flower mud of the control group showed white mold, while the flower mud of the embodiment showed normal color. After nine days, there was no obvious wilting phenomenon in both the control group and the embodiment. Around 12 days, more than 50% of the carnations in the control group showed senescence, mainly with curling, shrinking and fading of petals. At 15 days (such as Figure 2 As shown in Figure 2, the wilting rates of the flowers in Examples 1 to 4 were 20%, 26%, 13% and 6%, respectively.

[0054] Comparing Example 1 with Example 2, and Example 3 with Example 4, it is shown that increasing the dosage of the two fungicides, salicylic acid and alkyl polyglycosides can increase the degree of opening of carnations and extend their ornamental life.

[0055] Compared with Examples 1 and 2, the citric acid concentration in Examples 3 and 4 is doubled, and the pH is further reduced to about 3.5, which can also further extend the ornamental life of carnations.

[0056] Table 2

[0057]

[0058] It should be noted that the above-mentioned specific embodiments are exemplary, and those skilled in the art can come up with various solutions inspired by the disclosure of the present invention, and these solutions also belong to the disclosure scope of the present invention and fall within the protection scope of the present invention. Those skilled in the art should understand that the present invention specification and its drawings are illustrative and do not constitute a limitation of the claims. The scope of protection of the present invention is defined by the claims and their equivalents. The present invention specification contains multiple inventive concepts, such as "preferably", "according to a preferred embodiment" or "optionally", all of which indicate that the corresponding paragraph discloses an independent concept, and the applicant reserves the right to file a divisional application based on each inventive concept. Throughout the text, the features guided by "preferably" are only an optional method and should not be understood as being required. Therefore, the applicant reserves the right to abandon or delete the relevant preferred features at any time.

Claims

1. A preservative liquid for flower mud carnation cut flower shaping, characterized in that: The fresh-keeping liquid comprises a bactericide, an organic acid, a plant growth regulator, sucrose, a surfactant and water; wherein the bactericide is one or more selected from 8-hydroxyquinoline citrate, benzalkonium chloride and nanosilver; the organic acid is citric acid, the plant growth regulator is salicylic acid, and the surfactant is an alkyl polyglycoside.

2. The fresh-keeping liquid according to claim 1, characterized in that: The sucrose is used as an energy source.

3. The fresh-keeping liquid according to claim 1 or 2, characterized in that: The bactericide is at least two selected from 8-hydroxyquinoline citrate, benzalkonium chloride and nano silver.

4. The fresh-keeping liquid according to any one of claims 1 to 3, characterized in that The bactericide is a combination of 8-hydroxyquinoline citrate, benzalkonium chloride and nano silver.

5. The fresh-keeping liquid according to any one of claims 1 to 4, characterized in that: The fresh-keeping solution comprises 100-200 mg / L of 8-hydroxyquinoline citrate, 10-50 mg / L of benzalkonium chloride, 5-50 mg / L of nano silver, 100-500 mg / L of citric acid, 10-40 mg / L of salicylic acid, 10-40 mg / L of alkyl polyglycosides and 10-30 g / L of sucrose.

6. The fresh-keeping liquid according to claim 5, characterized in that: The fresh-keeping solution comprises 100-200 mg / L of 8-hydroxyquinoline citrate, 10-50 mg / L of benzalkonium chloride, 5-50 mg / L of nano silver, 100-500 mg / L of citric acid, 10-40 mg / L of salicylic acid, 10-40 g / L of alkyl polyglycosides, 10-30 g / L of sucrose and the balance of water.

7. The fresh-keeping liquid according to any one of claims 1 to 6, characterized in that: The total amount of the nano silver and salicylic acid is no more than 50 mg / L.

8. The fresh-keeping liquid according to any one of claims 1 to 7, characterized in that: The fresh-keeping solution comprises 200 mg / L of 8-hydroxyquinoline citrate, 50 mg / L of benzalkonium chloride, 10 mg / L of nanosilver, 500 mg / L of citric acid, 40 mg / L of salicylic acid, 30 μl / L of alkyl polyglycosides and 10 g / L of sucrose.

9. The fresh-keeping liquid according to claim 8, characterized in that: The fresh-keeping solution comprises 200 mg / L 8-hydroxyquinoline citrate, 50 mg / L benzalkonium chloride, 10 mg / L nanosilver, 500 mg / L citric acid, 40 mg / L salicylic acid, 30 μl / L alkyl polyglycoside, 10 g / L sucrose and the balance water.

10. Use of the preservative liquid according to any one of claims 1 to 9 in preserving cut flowers shaped as floral mud carnation cut flowers.