Yeast composite powder for enhancing immunity and preparation method thereof

Abstract

The invention relates to the technical field of biology, and particularly discloses yeast composite powder capable of enhancing immunity and a preparation method of the yeast composite powder. The invention discloses yeast composite powder for enhancing immunity. Comprising the following raw materials in parts by weight: 2.5-3.5 parts of casein phosphopeptides, 700-800 parts of whey protein concentrate, 25-35 parts of Epicor yeast powder, 20-30 parts of elderberry concentrate powder, 20-30 parts of acerola cherry concentrate powder, 20-25 parts of yeast beta glucan, 5-15 parts of whey protein isolate, 5-10 parts of stachyose, 5-10 parts of spirulina platensis, 4-6 parts of sialic acid, 4-6 parts of fructo-oligosaccharide, 2-3 parts of taurine and 2-3 parts of L-lysine. And 2-3 parts of vitamin C. The composition provided by the invention can be used for enhancing immunity, and has good anti-oxidation and anti-inflammatory capacities.

Description

Technical Field

[0001] The present application relates to the field of biotechnology, and more specifically, to a yeast compound powder for enhancing immunity and a preparation method thereof. Background Art

[0002] With the accelerated pace of modern life and the intensification of environmental pollution, the human body is facing more and more health challenges. Improving the body's anti-inflammatory, antioxidant and immune capabilities has become an important issue of general concern to the public. Improving immunity not only helps to resist the invasion of viruses and bacteria, but also effectively prevents and alleviates a variety of chronic diseases.

[0003] Currently, there are many ways to improve immunity, including a proper diet, moderate exercise, adequate sleep, and the use of drugs and health products. Among them, consuming foods or health products that have the function of enhancing immunity is widely favored because of its convenience and pertinence.

[0004] Yeast compound powder, as a new type of immune enhancer, has significant advantages. Yeast, as a common microorganism, contains rich nutrients, including protein, vitamins, minerals, sugar, fat, amino acids, etc. These ingredients play an important role in maintaining normal physiological functions of the human body and improving immunity.

[0005] Yeast compound powder, by fermenting yeast with other ingredients with anti-inflammatory and antioxidant functions (such as plant extracts, antioxidants, etc.), can not only improve the utilization rate of nutrients in yeast, but also produce more biologically active substances, thereby further enhancing its immune regulatory function.

[0006] However, there are many types of yeast compound powder products on the market, with uneven quality, and different preparation processes and formulas. Some products may have limited immune enhancement effects or even adverse effects on the human body due to improper preparation processes or unreasonable formulas.

[0007] Therefore, it is particularly important to develop a yeast compound powder and a preparation method thereof with scientific preparation technology, reasonable formula and significant immune enhancement effect. Summary of the invention

[0008] In order to improve the body's anti-inflammatory and antioxidant capabilities and enhance immunity, the present application provides a yeast compound powder for enhancing immunity and a preparation method thereof.

[0009] On the one hand, the present application provides a yeast compound powder for enhancing immunity, which adopts the following technical solution: A yeast compound powder for enhancing immunity, comprising the following raw materials in parts by weight: 2.5-3.5 parts of casein phosphopeptide, 700-800 parts of concentrated whey protein, 25-35 parts of Epicor yeast powder, 20-30 parts of elderberry concentrated powder, 20-30 parts of acerola concentrated powder, 20-25 parts of yeast beta glucan, 5-15 parts of isolated whey protein, 5-10 parts of stachyose, 5-10 parts of Spirulina platensis, 4-6 parts of sialic acid, 4-6 parts of oligofructose, 2-3 parts of taurine, 2-3 parts of L-lysine, and 2-3 parts of vitamin C.

[0010] By adopting the above technical solutions, whey protein and casein phosphopeptide as key nutrients not only provide rich nutritional support, but also effectively promote calcium absorption and enhance the activity of immune cells. At the same time, the synergistic effect of Epicor yeast powder, elderberry concentrate powder and acerola concentrate powder can stimulate the body's natural defense mechanism and further enhance the immune system. In addition, the addition of yeast beta glucan and isolated whey protein accelerates the production of antibodies and significantly improves the speed and efficiency of immune response.

[0011] Taurine and L-lysine have a positive effect on improving liver function. They can promote the maturation of immune cells and provide strong support for the normal functioning of the immune system. At the same time, a variety of nutrients such as stachyose, spirulina platensis, oligofructose, sialic acid, etc. are also involved, providing comprehensive nutritional support for the immune system and further enhancing immunity.

[0012] At the same time, the anthocyanins, polyphenols, minerals and flavonoids contained in elderberry concentrate powder and acerola concentrate powder have powerful antioxidant and anti-inflammatory effects, which can scavenge free radicals, reduce cell damage, inhibit inflammatory responses, reduce the burden on the body, and enhance the immune system response. However, anthocyanins and polyphenols are unstable and easily lost during storage and transportation, which reduces biological activity and affects product effects. Therefore, whey protein with a unique molecular structure is added, which has hydrophilic groups on the surface and is relatively hydrophobic inside. This structure allows milk protein to act like a "molecular container" to embed the active ingredients in elderberry concentrate powder and acerola concentrate powder inside, thereby effectively isolating adverse factors in the external environment. The embedded anthocyanin molecules are protected by milk protein, reducing direct contact with the external environment, thereby reducing the risk of thermal degradation, oxidative degradation and photodegradation, and improving the stability of the active ingredients in the plant concentrate powder. In summary, this application has constructed a comprehensive and synergistic nutrition system by carefully matching high-quality proteins such as whey protein, casein phosphopeptide, Epicor yeast powder, elderberry and acerola cherry concentrate powder, and auxiliary nutrients such as yeast beta glucan and taurine. This system not only provides a rich nutritional basis for immune cells, but also promotes calcium absorption, accelerates antibody production, stimulates natural defense mechanisms and improves liver function through the synergistic effects of antioxidant ingredients, anti-inflammatory ingredients, etc. These combined effects work together on multiple levels of the immune system, significantly enhancing the body's immune response ability and overall resistance, which is particularly suitable for people whose immunity needs to be improved, and achieves a multi-dimensional and deep immune enhancement effect.

[0013] Optionally, the elderberry concentrated powder is prepared by the following method: (1) freeze-drying elderberry and grinding it into fine powder for later use; (2) Take an appropriate amount of elderberry powder, add acidified glycerol and methanol, and ultrasonicate for 30-40 minutes, filter and extract 2-3 times, and combine the filtrate; (3) adding an ionic liquid aqueous solution to the filter residue, stirring the reaction in an oil bath at 60-70° C. for 20-30 min, filtering, and combining the resultant solution with the filtrate obtained in step (2); (4) The filtrate is vacuum concentrated to a relative density of 1.10-1.25, and then the concentration is stopped and the filtrate is allowed to cool to room temperature to obtain a concentrated extract, which is freeze-dried, crushed, and sieved to obtain the elderberry concentrated powder.

[0014] By adopting the above technical scheme, the cavitation effect of ultrasound can destroy the cell wall and promote the release of active ingredients. Acidified glycerol and methanol are used as solvents to help improve the extraction efficiency and efficiently extract target substances such as anthocyanins in elderberry while maintaining the stability of the extract. Filter and extract 2-3 times, and combine the filtrate to collect as many active ingredients as possible. Further add ionic liquid to further extract active ingredients such as flavonoids remaining in the filter residue, and ensure that all active ingredients are effectively collected after merging. Using this extraction method not only improves the extraction efficiency and shortens the extraction time, but also ensures the activity of anthocyanins, flavonoids and other substances during the extraction process, so that they are protected from adverse factors such as oxidation and degradation. Finally, the elderberry concentrated powder with higher activity and more beneficial ingredients is extracted, thereby improving the beneficial effects of the yeast compound powder.

[0015] Optionally, in the preparation step, the weight ratio of elderberry powder to acidified glycerol and methanol is 1:1-1.5:1.4-2.

[0016] By adopting the above technical scheme, the acidified glycerin has a certain antioxidant property, and can protect the active ingredients in the elderberry from oxidative damage during the extraction process. By setting a reasonable ratio, it can be ensured that the acidified glycerin fully infiltrates the elderberry powder, thereby more effectively extracting the active ingredients therein.

[0017] Optionally, the acidified glycerol is prepared by mixing glycerol and citric acid in a weight ratio of 1:3-5.

[0018] By adopting the above technical solution, the citric acid in the acidified glycerol can destroy the cell structure of elderberry, making the active ingredients more easily released. Glycerol, as a solvent, can increase the polarity of the solution, which is helpful for the dissolution and extraction of the active ingredients in elderberry. By adjusting the weight ratio of glycerol to citric acid, an acidified glycerol solution with specific polarity and acidity is prepared. This solution can better meet the extraction requirements of active ingredients with different polarities in elderberry, thereby improving the extraction efficiency and product quality.

[0019] Optionally, the acidified glycerol is prepared by the following steps: Weigh corresponding mass parts of glycerol and organic acid, mix them, heat them in an oil bath at 100-120° C., and stir them continuously during the heating process until the organic acid is completely dissolved in the glycerol, so as to prepare the acidified glycerol.

[0020] By adopting the above technical solution, heating the oil bath to 80-100°C can accelerate the dissolution process of the organic acid in the glycerol, making the preparation process more efficient. Continuous stirring helps to ensure the uniform distribution of the organic acid in the glycerol, further improving the dissolution efficiency.

[0021] Optionally, the ionic liquid aqueous solution is an ionic liquid aqueous solution containing any one of 1-butyl-3-methylimidazolium chloride or 1-butyl-3-methylimidazolium tetrafluoroborate, and the mass concentration of the ionic liquid aqueous solution is 20%-30%.

[0022] By adopting the above technical scheme, the two ionic liquids can effectively dissolve flavonoids and minerals in elderberry, improve the extraction efficiency while maintaining the stability of these active ingredients, and avoid unnecessary degradation and transformation. At the same time, the ionic liquids can exchange ions with the ionic components in elderberry, which helps to separate the target components from the complex matrix and increase the content of active ingredients with immune-enhancing properties in the extracted elderberry concentrated powder, thereby enhancing the immune-enhancing effect of the yeast compound powder.

[0023] Optionally, the raw materials further include 0.5-1 parts of an antioxidant, and the antioxidant is any one of vitamin E and resveratrol.

[0024] By adopting the above technical solution, vitamin E and resveratrol can improve the intestinal environment and promote the growth of beneficial bacteria, thereby facilitating the absorption and utilization of nutrients and improving the effect of yeast compound powder.

[0025] In a second aspect, the present application provides a method for preparing a yeast composite powder for enhancing immunity, using the following technical solution: A method for preparing yeast composite powder for enhancing immunity comprises the following steps: (1) According to the weight ratio of claim 1, each raw material is accurately weighed, and the concentrated whey protein and the isolated whey protein are dissolved in water to form a uniform whey protein solution, and then the concentrated elderberry powder and the concentrated acerola cherry powder are added and mixed thoroughly, and then spray-dried after homogenization; (2) The powder obtained after the spray drying is uniformly mixed with other raw materials, dried, and the moisture content is controlled to be ≤5%, and then sieved to obtain yeast composite powder.

[0026] By adopting the above technical solution, whey protein concentrate and whey protein isolate are dissolved in water to form a uniform solution, and then elderberry concentrated powder and acerola concentrated powder are added and fully mixed, which can ensure that the whey protein effectively wraps the active ingredients of the two concentrated powders to form a dense protective film. This wrapping effect not only improves the stability of the active ingredients, but also reduces the loss of the active ingredients during processing and storage, ensuring the product quality of the yeast compound powder.

[0027] In summary, this application has the following beneficial effects: 1. This application carefully proportions a variety of nutrients, such as whey protein, casein phosphopeptide, Epicor yeast powder, etc. These ingredients can work synergistically in the human body to provide comprehensive nutritional support for immune cells. At the same time, the antioxidant and anti-inflammatory components in elderberry concentrate powder and acerola concentrate powder further enhance the defense capabilities of the immune system. This comprehensive nutritional system not only improves the speed and efficiency of the immune response, but also significantly enhances the body's overall resistance, providing an effective nutritional supplement for people whose immunity needs to be improved.

[0028] 2. The present application preferably adopts the ultrasonic cavitation effect, the synergistic effect of acidified glycerol and methanol, and the further extraction of ionic liquid, which significantly improves the extraction efficiency, shortens the extraction time, and ensures the activity of anthocyanins, flavonoids and other substances during the extraction process. This efficient extraction method makes the final elderberry concentrated powder have a higher content of active ingredients, thereby enhancing the immune enhancement effect of the yeast compound powder.

[0029] 3. The method of the present application can ensure that the whey protein effectively wraps the active ingredients of the two concentrated powders to form a dense protective film by dissolving the concentrated whey protein and the isolated whey protein in water to form a uniform solution, and then adding the concentrated elderberry powder and the concentrated acerola cherry powder to mix them thoroughly. This wrapping effect not only improves the stability of the active ingredients, reduces the loss of the active ingredients during processing and storage, but also ensures the product quality of the yeast compound powder. DETAILED DESCRIPTION

[0030] The present application is further described in detail below with reference to the embodiments.

[0031] If the specific conditions are not specified in the examples, the experiments were carried out under conventional conditions or conditions recommended by the manufacturer. If the manufacturers of the reagents or instruments are not specified, they are all conventional products that can be purchased commercially.

[0032] Preparation Example of Elderberry Concentrated Powder Preparation Example 1 A concentrated elderberry powder, the preparation of which comprises the following steps: (1) Wash and dry fresh elderberries, freeze-dry them in a vacuum, grind them into fine powder, and sieve them through a 10-mesh sieve for later use; (2) Weigh 10 g of glycerol and 30 g of citric acid, mix them, heat them in an oil bath to 100° C., and stir them continuously during the heating process until the citric acid is completely dissolved in the glycerol to obtain the acidified glycerol. Take 10 g of elderberry powder, add 12.5 g of acidified glycerol and 20 g of methanol, sonicate at 200 W for 30 min, filter and extract 3 times, and combine the filtrates; (3) Dissolve 5 g of 1-butyl-3-methylimidazole chloride in 20 g of deionized water, mix with the remaining filter residue, heat to 65° C. in an oil bath, stir and dissolve for 20 min, filter, and combine with the filtrate obtained in step (2); (4) The filtrate was concentrated under vacuum, the vacuum degree was controlled at -0.07 MPa, and the relative density was 1.239 g / cm at 75°C. 3 The concentration was stopped at 40°C and the mixture was allowed to cool to room temperature to obtain a concentrated extract, which was freeze-dried, crushed and passed through a 40-mesh sieve to obtain the elderberry concentrated powder.

[0033] Preparation Example 2 A concentrated elderberry powder, the preparation of which comprises the following steps: (1) Wash and dry fresh elderberries, freeze-dry them in a vacuum, grind them into fine powder, and sieve them through a 10-mesh sieve for later use; (2) Weigh 10 g of glycerol and 40 g of citric acid, mix them, heat them in an oil bath to 120° C., and stir them continuously during the heating process until the citric acid is completely dissolved in the glycerol to obtain the acidified glycerol. Take 10 g of elderberry powder, add 10 g of acidified glycerol and 17 g of methanol, sonicate at 200 W for 30 min, filter and extract 3 times, and combine the filtrates; (3) Dissolve 4 g of 1-butyl-3-methylimidazolium tetrafluoroborate in 20 g of deionized water, mix with the remaining filter residue, heat to 65° C. in an oil bath, stir and dissolve for 20 min, filter, and combine with the filtrate obtained in step (2); (4) The filtrate was concentrated under vacuum, the vacuum degree was controlled at -0.07 MPa, and the relative density was 1.239 g / cm at 75°C. 3 The concentration was stopped at 40°C and the mixture was allowed to cool to room temperature to obtain a concentrated extract, which was freeze-dried, crushed and passed through a 40-mesh sieve to obtain the elderberry concentrated powder.

[0034] Preparation Example 3 A concentrated elderberry powder, the preparation of which comprises the following steps: (1) Wash and dry fresh elderberries, freeze-dry them in a vacuum, grind them into fine powder, and sieve them through a 10-mesh sieve for later use; (2) Weigh 10 g of glycerol and 50 g of citric acid, mix them, heat them in an oil bath to 110° C., and stir them continuously during the heating process until the citric acid is completely dissolved in the glycerol to obtain the acidified glycerol. Take 10 g of elderberry powder, add 15 g of acidified glycerol and 14 g of methanol, sonicate at 200 W for 30 min, filter and extract 3 times, and combine the filtrates; (3) Dissolve 6 g of 1-butyl-3-methylimidazole chloride in 20 g of deionized water, mix with the remaining filter residue, heat to 65° C. in an oil bath, stir and dissolve for 20 min, filter, and combine with the filtrate obtained in step (2); (4) The filtrate was concentrated under vacuum, the vacuum degree was controlled at -0.07 MPa, and the relative density was 1.239 g / cm at 75°C. 3 The concentration was stopped at 40°C and the mixture was allowed to cool to room temperature to obtain a concentrated extract, which was freeze-dried, crushed and passed through a 40-mesh sieve to obtain the elderberry concentrated powder.

[0035] Preparation Example 4 A concentrated elderberry powder, which is different from Preparation Example 1 in that the weight ratio of elderberry powder to acidified glycerol and methanol is 1:2:1, that is, 10g of elderberry powder, 20g of acidified glycerol and 10kg of methanol are added, and the remaining preparation steps are the same as Preparation Example 1.

[0036] Preparation Example 5 A concentrated elderberry powder, which is different from Preparation Example 1 in that the weight ratio of elderberry powder to acidified glycerol and methanol is 1:0.5:3, that is, 10g of elderberry powder, 5g of acidified glycerol and 30kg of methanol are added, and the remaining preparation steps are the same as Preparation Example 1.

[0037] Preparation Example 6 A concentrated elderberry powder is different from Preparation Example 1 in that the ionic liquid added in this Preparation Example is 1-octyl-3-methylimidazole chloride.

[0038] Preparation Example 7 A concentrated elderberry powder is different from Preparation Example 1 in that the acidified glycerin in this Preparation Example is obtained by mixing glycerin and oxalic acid in a weight ratio of 1:3.

[0039] Comparative Preparation Example 1 An elderberry concentrated powder, which is different from Preparation Example 1 in that glycerol is used as an extraction solvent in this Preparation Example, and the preparation comprises the following steps: (1) Wash and dry fresh elderberries, freeze-dry them in a vacuum, grind them into fine powder, and sieve them through a 10-mesh sieve for later use; (2) Take 10 g of elderberry powder, add 32.5 g of glycerol, sonicate at 200 W for 30 min, filter and extract three times, and combine the filtrates; (3) Dissolve 5 g of 1-butyl-3-methylimidazole chloride in 20 g of deionized water, mix with the remaining filter residue, heat to 65° C. in an oil bath, stir and dissolve for 20 min, filter, and combine with the filtrate obtained in step (2); (4) After the filtrate is vacuum concentrated, the vacuum degree is controlled to be -0.07 MPa, and the concentration is stopped when the relative density is 1.239 g / cm3 at 75°C, and the filtrate is allowed to cool to room temperature to obtain a concentrated extract, which is freeze-dried and crushed through a 40-mesh sieve to obtain the elderberry concentrated powder.

[0040] Comparative Preparation Example 2 A concentrated elderberry powder is different from comparative preparation example 1 in that ethanol is used as an extraction solvent instead of glycerol in this preparation example, and the remaining steps are the same.

[0041] Comparative Preparation Example 3 A concentrated elderberry powder is different from Preparation Example 1 in that no ionic liquid aqueous solution is used for extraction in this comparative preparation example, and the remaining steps are the same. Example

[0042] Example 1 A yeast compound powder for enhancing immunity, the preparation of which comprises the following steps: (1) accurately weighing each raw material, dissolving whey protein concentrate and whey protein isolate in an appropriate amount of water to form a uniform whey protein solution, adding elderberry concentrated powder and acerola concentrated powder and mixing thoroughly, homogenizing the mixture with a homogenizer, and then spray drying; (2) The powder obtained after the spray drying was mixed evenly with other raw materials, dried at 50° C., and the moisture content was controlled to be ≤5%, and then passed through a 40-mesh sieve to obtain a yeast composite powder. The elderberry concentrated powder was prepared in Preparation Example 1.

[0043] Example 2-3 A yeast composite powder for enhancing immunity, which is different from Example 1 in that the raw material components and their corresponding weight portions are shown in Table 1, wherein the elderberry concentrated powder used in Example 2 is prepared by Preparation Example 2, and the elderberry concentrated powder used in Example 3 is prepared by Preparation Example 3.

[0044] Table 1 Raw materials and their weights in Examples 1-3 (mg) Example 4 A yeast composite powder for enhancing immunity, which is different from Example 1 in that the elderberry concentrated powder used in this example is prepared in Preparation Example 4.

[0045] Example 5 A yeast composite powder for enhancing immunity, which is different from Example 1 in that the elderberry concentrated powder used in this example is prepared in Preparation Example 5.

[0046] Example 6 A yeast composite powder for enhancing immunity, which is different from Example 1 in that the elderberry concentrated powder used in this example is prepared in Preparation Example 6.

[0047] Example 7 A yeast composite powder for enhancing immunity, which is different from Example 1 in that the elderberry concentrated powder used in this example is prepared in Preparation Example 7.

[0048] Example 8 A yeast composite powder for enhancing immunity, which is different from Example 1 in that 0.5 kg of vitamin E is also added in this example.

[0049] Example 9 A yeast composite powder for enhancing immunity, which is different from Example 1 in that 1 kg of resveratrol is also added in this example.

[0050] Comparative Example Comparative Example 1 A yeast composite powder for enhancing immunity, which is different from Example 1 in that the elderberry concentrated powder added in this comparative example is prepared in Comparative Preparation Example 1.

[0051] Comparative Example 2 A yeast composite powder for enhancing immunity, which is different from Example 1 in that the elderberry concentrated powder added in this comparative example is prepared in Comparative Preparation Example 2.

[0052] Comparative Example 3 A yeast composite powder for enhancing immunity, which is different from Example 1 in that the elderberry concentrated powder added in this comparative example is prepared in Comparative Preparation Example 3.

[0053] Comparative Example 4 A yeast composite powder for enhancing immunity, which is different from Example 1 in that the preparation thereof comprises the following steps: Accurately weigh the mass of each raw material, mix them evenly, dry them at 50° C., control the moisture content to ≤5%, and then pass them through a 40-mesh sieve to obtain yeast composite powder. The elderberry concentrated powder is prepared in Preparation Example 1.

[0054] Performance testing Detection method / test method Antioxidant activity: The DPPH scavenging rate IC50 value was used to characterize the antioxidant activity of the extract. i :Take 2mL sample and 2mL DPPH solution into a test tube; A j :Put 2mL of sample and 2mL of anhydrous ethanol in a test tube; A c : Take 2 mL of anhydrous ethanol and 2 mL of DPPH solution in three groups and vibrate for 10 seconds. After keeping in a 37°C incubator for 30 minutes, use a spectrophotometer to measure the absorbance of the sample at 517 nm. Calculate the DPPH clearance rate (denoted as F, %) according to the following formula: Anti-inflammatory effect: Purchased male ICR mice were randomly divided into an experimental group (20 mL / kg of the yeast compound powder prepared in this application) and a blank group (20 mL / kg of normal saline), and the drug was administered continuously for 7 days. One hour after the last administration, 20 μL of xylene was evenly applied on the front and back of the left ear of the mouse to induce inflammation. After 30 minutes, blood was collected from the eye canthus of the mouse, and centrifuged at 3500 r / min at 4°C for 10 minutes to separate the serum. The ELISA method was used to detect the content of TNF-α and IL-1β. The specific method is shown in the instruction manual of the kit; Storage stability test: The yeast compound powder remaining from the above tests was sealed and stored for 30 days under normal conditions. Then, the DPPH clearance rate, TNF-α and IL-1β contents were tested again according to the above method.

[0055] Table 2 Test results Table 3 Test results after storage at room temperature for 30 days DPPH removal rate / % TNF-α(pg / mL) IL-1β (pg / mL) Blank Group 43.98 27.12 10.23 Example 1 74.12 15.67 5.34 Example 2 71.23 17.09 6.08 Example 3 72.87 16.12 5.76 Example 4 67.56 18.45 6.89 Example 5 65.98 19.34 7.32 Example 6 68.98 17.87 6.43 Example 7 69.34 18.12 6.23 Example 8 77.34 14.45 4.98 Example 9 76.98 14.98 5.08 Comparative Example 1 59.12 22.67 8.89 Comparative Example 2 58.45 23.34 9.32 Comparative Example 3 58.98 22.98 9.08 Comparative Example 4 64.87 23.12 8.78 Combining Examples 1-3 and Comparative Examples 1-3 and Table 2, it can be seen that the experimental data of Examples 1-3 are better than those of Comparative Examples 1-3, indicating that the elderberry concentrated powder extracted by the extraction method provided in the present application contains more effective ingredients, and the yeast composite powder prepared therefrom has more significant anti-inflammatory and antioxidant effects and better stability.

[0056] Combining Examples 1-3 and Comparative Example 4 and Table 2, it can be seen that the experimental data of Examples 1-3 are better than those of Comparative Example 4, indicating that the yeast composite powder prepared by the method of the present application has better stability, and its effect remains unchanged before and after storage, and still has good anti-inflammatory and antioxidant effects.

[0057] Combining Examples 1-5 and Table 2, it can be seen that the experimental data of Examples 1-3 are better than those of Examples 4-5, indicating that the amount of acidified glycerol and methanol added during the extraction process affects the final extraction effect. Reasonable addition amounts can optimize the extraction process and increase the content of effective ingredients, thereby further improving the quality of the yeast compound powder.

[0058] Combining Example 1 with Example 6 and Table 2, it can be seen that the experimental data of Example 1 are better than those of Example 6, indicating that the selection of the ionic liquid affects the extraction effect of the final elderberry concentrated powder, and more beneficial components in the elderberry powder can be extracted, thereby improving the quality of the yeast composite powder.

[0059] Combining Example 1 with Example 7 and Table 2, it can be seen that the experimental data of Example 1 are better than those of Example 7, indicating that the selection of organic acid in acidified glycerol is also crucial. Selecting citric acid as the organic acid can more effectively promote the extraction process and increase the content of the active ingredient in the elderberry concentrated powder.

[0060] Combining Example 1 with Examples 8-9 and Table 2, it can be seen that the experimental data of Examples 8-9 are better than those of Example 1, indicating that further adding vitamin E or resveratrol to the raw materials can improve the body's absorption of the yeast compound powder, thereby improving the anti-inflammatory effect of the yeast compound powder.

[0061] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A yeast compound powder for enhancing immunity, characterized in that: The composition comprises the following raw materials in parts by weight: 2.5-3.5 parts of casein phosphopeptide, 700-800 parts of concentrated whey protein, 25-35 parts of Epicor yeast powder, 20-30 parts of elderberry concentrated powder, 20-30 parts of acerola concentrated powder, 20-25 parts of yeast beta glucan, 5-15 parts of isolated whey protein, 5-10 parts of stachyose, 5-10 parts of Spirulina platensis, 4-6 parts of sialic acid, 4-6 parts of oligofructose, 2-3 parts of taurine, 2-3 parts of L-lysine, and 2-3 parts of vitamin C.

2. The yeast composite powder for enhancing immunity according to claim 1, characterized in that: The elderberry concentrated powder is prepared by the following method: (1) Freeze-dry the elderberry and grind it into fine powder for later use; (2) Take an appropriate amount of elderberry powder, add acidified glycerol and methanol, ultrasonicate for 30-40 minutes, filter and extract 2-3 times, and combine the filtrate; (3) adding an ionic liquid aqueous solution to the filter residue, stirring the mixture in an oil bath at 60-70° C. for 20-30 min, filtering the mixture, and combining the mixture with the filtrate obtained in step (2); (4) The filtrate is vacuum concentrated to a relative density of 1.10-1.25, and then the concentration is stopped and allowed to cool to room temperature to obtain a concentrated extract, which is freeze-dried, crushed, and sieved to obtain the elderberry concentrated powder.

3. The yeast composite powder for enhancing immunity according to claim 2, characterized in that: In the preparation step, the weight ratio of elderberry powder to acidified glycerol and methanol is 1:1-1.5:1.4-2.

4. The yeast composite powder for enhancing immunity according to claim 2, characterized in that: The acidified glycerol is prepared by mixing glycerol and citric acid in a weight ratio of 1:3-5.

5. The yeast composite powder for enhancing immunity according to claim 4, characterized in that: The acidified glycerol is prepared by the following steps: Weigh corresponding mass parts of glycerol and organic acid, mix them, heat them in an oil bath at 100-120° C., and stir them continuously during the heating process until the organic acid is completely dissolved in the glycerol, so as to obtain the acidified glycerol.

6. The yeast composite powder for enhancing immunity according to claim 1, characterized in that: The ionic liquid aqueous solution is an ionic liquid aqueous solution containing any one of 1-butyl-3-methylimidazolium chloride and 1-butyl-3-methylimidazolium tetrafluoroborate, and the mass concentration of the ionic liquid aqueous solution is 20%-30%.

7. The yeast composite powder for enhancing immunity according to claim 1, characterized in that: The raw materials also include 0.5-1 part of an antioxidant, and the antioxidant is any one of vitamin E and resveratrol.

8. A method for preparing the yeast composite powder for enhancing immunity according to any one of claims 1 to 7, characterized in that: The steps include: (1) According to the weight ratio of claim 1, accurately weigh the raw materials, dissolve the concentrated whey protein and the isolated whey protein in water to form a uniform whey protein solution, add the concentrated elderberry powder and the concentrated acerola cherry powder, mix thoroughly, homogenize, and then spray dry; (2) The powder obtained after the spray drying is evenly mixed with other raw materials, dried, and the moisture content is controlled to be ≤5%, and then sieved to obtain yeast composite powder.

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