A system for intelligent monitoring of gastrointestinal chyme emptying signals in ruminants

Abstract

The present invention relates to a system for intelligently monitoring gastrointestinal chyme emptying signals of ruminants, which collects electrical signals in the gastrointestinal tract of ruminants by preparing the resistance change of the active conductive layer of dimolybdenum trisulfide Mo2S3 nanowire network in a flexible stretchable strain sensor, and then stores and analyzes the collected data through an information transmission unit and a data analysis unit. The system provided by the present invention senses the changes in the gastrointestinal motility of ruminants through the stretchable strain sensor, and converts these changes into readable electrical signals, has better biocompatibility, can realize real-time monitoring of the gastrointestinal motility signals of ruminants, and provides a research basis for obtaining the specific chyme emptying rules of the abomasum and small intestine of ruminants.

Description

Technical Field

[0001] The invention relates to the technical field of animal experiments, and in particular to a system for intelligently monitoring gastrointestinal chyme emptying signals of ruminants. Background Art

[0002] In ruminant nutrition, post-rumen nutritional regulation is closely related to the emptying rules of the abomasum and small intestine. Due to the unique compound stomach digestion and metabolic system, the emptying rules of the abomasum and small intestine of ruminants are significantly different from those of monogastric animals. Studies have shown that the inflow and emptying of abomasum chyme is continuous throughout the day, and only stagnates briefly before rumination or under stress conditions, while the inflow and emptying of chyme in the stomach of monogastric animals is only during the feeding stage.

[0003] Studies have described the physiological activities of the abomasum and duodenum: the abomasum transports food to the pyloric antrum, where abomasum emptying occurs. The pyloric antrum is then responsible for further transporting food to the duodenum through coordinated contraction when the pylorus opens. The duodenum further transports the intake downward to the intestine. The cholinergic, muscarinic and nicotinic parts of the nervous system in the abomasum and small intestine wall produce and control electrical processes. The receptors in the gastrointestinal wall can detect tension and thus detect the fullness of the organs and control the emptying rate. This process is called the minute rhythm. Due to the limitations of research methods, research on the continuity of the emptying rules of the abomasum and small intestine chyme and the differences in emptying rules under different physiological states is in a vague state and there are many controversies. Therefore, clarifying the emptying rules of the abomasum and small intestine chyme can enable researchers to have a more specific understanding of the digestive physiological characteristics of ruminants, and to have a more comprehensive regulation of the nutrition of the ruminant digestive tract, which can provide a solid research foundation for in vitro artificial abomasums and artificial intestines.

[0004] Initially, the emptying of chyme in the abomasum and duodenum was studied by implanting cannulas in the abomasum and duodenum to measure the flow and volume of chyme discharged; however, researchers believe that this method has great defects. The penetration of the intestinal wall by the cannula will damage the enteric nervous system and affect the emptying pattern. Therefore, some scholars have proposed the method of recording and analyzing gastrointestinal motility by electromyography. This method does not damage the enteric nervous system, but there is no consistent explanation for the correlation between the electrical signal and the contraction of the gastric muscle layer. The peak is considered to be the mixed chyme in the abomasum, not for transporting the chyme to the duodenum. It is also found that the myoelectric activity in the pyloric antrum is responsible for transport to the small intestine.

[0005] With the development of radiological examination technology, researchers prefer non-invasive methods to study the emptying of gastrointestinal chyme. Radiological examinations using barium sulfate as a contrast agent in animals are used to measure the pattern of gastrointestinal chyme emptying. This method has limitations and can only provide qualitative results. Other diagnostic techniques, such as recording and analyzing stomach and intestinal sounds, computed tomography and ultrasound have been used in the study of gastrointestinal emptying in ruminants, but have problems such as short detection time, spatial limitations, and can only provide qualitative results, and cannot specifically explain the pattern of gastrointestinal chyme emptying. Summary of the invention

[0006] With the continuous innovation and development of technologies such as flexible electronic skin, multifunctional device integration, and artificial intelligence, flexible electronic skin sensors are gradually becoming intelligent, diversified, and miniaturized. They can sense changes in the external environment, such as changes in pressure, temperature, humidity, or chemical substances, and convert these changes into readable signals. Compared with traditional monitoring equipment, flexible electronic skin sensors are more sensitive in detecting electromyographic signals, electrocardiographic signals, and electroencephalographic signals. They have better biocompatibility in the study of in vitro cardiopulmonary motion signal detection, and can achieve real-time monitoring of the movement status of organs. There are no reports on the use of flexible electronic skin sensors to monitor gastrointestinal motility in vivo. Because of its high sensitivity, fast response speed, good biocompatibility with in vivo tissues, stability and reliability, it is an ideal choice for studying the law of gastrointestinal chyme emptying in vivo.

[0007] In view of this, the present invention provides a system for intelligently monitoring gastrointestinal chyme emptying signals of ruminants, comprising an information collection unit, an information transmission unit and a data analysis unit;

[0008] The information acquisition unit is a flexible stretchable strain sensor made by S1;

[0009] The fabrication of a flexible stretchable strain sensor involves the following steps:

[0010] S101, in molybdenum disulfide MoS 2 Intermediate Insertion 2 +S] chain to obtain molybdenum trisulfide Mo 2 S 3 Nanowires;

[0011] Preparation of Mo using liquid-liquid interface self-assembly method 2 S 3 Nanowire mesh, high piezoresistive sensitivity (5.65 kPa-1), low pressure detection limit (0.08 Pa);

[0012] S102, using high-performance platinum-cured liquid silicone compounds to make an elastomeric film as a substrate for a flexible and stretchable strain sensor;

[0013] S103, making a flexible stretchable strain sensor electrode;

[0014] The required gold electrode is prepared by vacuum evaporation technology. 2 S 3 The nanowire network was transferred to the prepared elastic substrate and assembled with gold electrodes to obtain a flexible stretchable strain sensor;

[0015] S104, performing stability test on the completed stretchable strain sensor;

[0016] The information transmission unit is a Zigbee sub-node and a Zigbee master node of the host computer;

[0017] The data analysis unit includes an MSP430 single-chip microcomputer, a 485 chip, a host computer and a computer;

[0018] The MSP430 single-chip computer, 485 chip and Zigbee sub-node are integrated on the same circuit board to form a measurement node; after collecting data, it is transmitted to the Zigbee master node of the host computer through wireless communication technology, and the host computer and computer solve and graphically process the signal;

[0019] Preferably, the flexible stretchable strain sensor housing comprises a transparent silicone hollow ring.

[0020] Preferably, the morphology and structure of the flexible stretchable strain sensor material are characterized by field emission scanning electron microscopy and transmission electron microscopy combined with energy dispersion spectroscopy to perform stability testing, the electrochemical workstation records the electrical signal and stability of the flexible stretchable strain sensor, and the electrical signal and stability of the flexible stretchable sensor are determined in vitro by simulating the intestinal environment.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] The flexible stretchable strain sensor prepared by the present invention can sense changes in the environment, is more sensitive in detecting electromyographic signals, electrocardiographic signals and electroencephalographic signals, and converts these changes into readable signals, and has better biocompatibility, and can realize real-time monitoring of gastrointestinal motility signals of ruminants;

[0023] The system for intelligently monitoring ruminant gastrointestinal chyme emptying signals provided by the present invention provides a basis for studying the emptying rules of ruminant gastrointestinal chyme through data storage and data analysis. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] FIG1 is a block diagram of the system design for intelligent monitoring of gastrointestinal chyme emptying signals of ruminants according to the present invention;

[0025] Figure 2For the synthesis of Mo in the present invention 2 S 3 Nanowire process diagram;

[0026] Figure 3 This is a schematic diagram of a flexible stretchable strain sensor model of the present invention;

[0027] Figure 4 This is a flow chart for studying the emptying rules of chyme in the gastrointestinal tract of ruminants in this embodiment. DETAILED DESCRIPTION

[0028] In order to further explain the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the specific implementation mode, structure, characteristics and effects of the present invention are described in detail below in combination with the accompanying drawings and preferred embodiments.

[0029] In order to collect ruminant abomasum and small intestinal chyme emptying signals to obtain the emptying rules of ruminant abomasum and small intestinal chyme, the present invention provides a system for intelligently monitoring ruminant gastrointestinal chyme emptying signals, including an information collection unit, an information transmission unit and a data analysis unit;

[0030] The information acquisition unit is a flexible and stretchable strain sensor;

[0031] The fabrication of a flexible stretchable strain sensor involves the following steps:

[0032] The principle of flexible stretchable strain sensor is to use the active conductive layer of molybdenum trisulfide Mo in the sensor. 2 S 3 The resistance change of the nanowire network is used to detect the pulling force; Mo 2 S 3 Nanowires have high aspect ratios, better mechanical elasticity, and larger specific surface area, which facilitates their application as active conductive layers.

[0033] S101, in molybdenum disulfide MoS 2 Intermediate Insertion 2 +S] chain to obtain molybdenum trisulfide Mo 2 S 3 Nanowires;

[0034] like Figure 2 As shown, Mo was prepared using a liquid-liquid interface self-assembly method. 2 S 3 Nanowire mesh, high piezoresistive sensitivity (5.65 kPa-1), low pressure detection limit (0.08 Pa), high conductivity 4.9×104 S·m -1 and high aspect ratio (~200);

[0035] S102, using high-performance platinum-cured liquid silicone compounds to make an elastomeric film as a substrate for a flexible and stretchable strain sensor;

[0036] S103, making a flexible stretchable strain sensor electrode;

[0037] The required gold electrodes were prepared by vacuum evaporation technology, such as Figure 3 As shown, Mo 2 S 3 The nanowire network was transferred to the prepared elastic substrate and assembled with gold electrodes to obtain a flexible stretchable strain sensor;

[0038] S104, performing stability test on the completed stretchable strain sensor;

[0039] The morphology and structure of the flexible stretchable strain sensor were characterized by field emission scanning electron microscopy and transmission electron microscopy combined with energy dispersion spectroscopy. The electrical signal and stability of the flexible stretchable strain sensor were recorded by an electrochemical workstation. The electrical signal and stability of the flexible stretchable sensor were measured in vitro by simulating the intestinal environment.

[0040] The information transmission unit is the Zigbee sub-node and the Zigbee master node of the host computer;

[0041] The data analysis unit includes MSP430 single chip microcomputer, 485 chip, host computer and computer;

[0042] Integrate the MSP430 microcontroller, 485 chip and Zigbee subnode on the same circuit board to form a measurement node;

[0043] The flexible stretchable strain sensor is fixed between the abomasal antrum and duodenum of the monitored animal. The information acquisition unit obtains the real-time peristaltic signal of the gastrointestinal tract through the flexible stretchable strain sensor and converts it into an electrical signal. The Zigbee sub-node transmits the electrical signal to the Zigbee main node of the host computer through wireless communication technology. The data analysis unit uses the MSP430 microcontroller and 485 chip on the host computer to solve and graphically process the received signal, and processes the data through the wavelet threshold algorithm to extract the relevant parameters of the chyme emptying law, including the number of emptying times, emptying time, and emptying rate. The data can be stored, exported, and visualized as needed.

[0044] Real-time acquisition and testing of gastrointestinal motility signals of living ruminants:

[0045] Experimental animals and feeding management: 6 adult Hu sheep with a difference of less than 1 kg (3 males and 3 females), fed twice a day at 8 am and 6 pm, with free access to water;

[0046] Signal monitoring tool: It consists of a flexible ring-shaped stretchable sensor and a sensor protection shell (transparent silicone hollow ring);

[0047] In-body sensor installation process:

[0048] ①Fix the sensor between the abomasal antrum and duodenum, and use the two fixing lines on the sensor to pass through the mesentery for fixation;

[0049] ② Use the mesenteric fat layer to install and fix the sensor protective shell;

[0050] ③ The experiment started after the sheep returned to normal feed intake and was monitored for 48 hours;

[0051] Data collection: The computer automatically records sensor data;

[0052] Physiological status indicator collection: resting, eating, rumination (physiological status needs to be recorded in detail, every 15 minutes);

[0053] Data analysis: During the feeding phase, the average number of emptying of the abomasum was 9.54 times, and the average number of emptying of the duodenum was 9.47 times;

[0054] During the rumination phase, the average number of emptying of the abomasum was 5.12 times, and the average number of emptying of the duodenum was 5.55 times;

[0055] During the rest period, the average emptying frequency of the abomasum was 5.91 times, and the average emptying frequency of the duodenum was 5.42 times (daily concentrate to roughage ratio of 3:7; 15 minutes);

[0056] The emptying signal of the abomasum is eating > rumination and resting, and the emptying signal of the small intestine is eating > rumination and resting; overall, the abomasum is emptied more times than the small intestine; the emptying of the abomasum and small intestine is continuous, without long-term (more than 1 hour) pauses in emptying.

[0057] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A system for intelligently monitoring gastrointestinal chyme emptying signals of ruminants, characterized in that: It includes an information collection unit, an information transmission unit and a data analysis unit; The information acquisition unit is a flexible stretchable strain sensor; The fabrication of a flexible stretchable strain sensor involves the following steps: S101, inserting [Mo2+S] chain in the middle of molybdenum disulfide MoS2 to obtain molybdenum trisulfide Mo2S3 nanowires; Mo2S3 nanowire grids were prepared using a liquid-liquid interface self-assembly method; S102, using high-performance platinum-cured liquid silicone compounds to make an elastomeric film as a substrate for a flexible and stretchable strain sensor; S103, making a flexible stretchable strain sensor electrode; The required gold electrode is prepared by vacuum evaporation technology, and the Mo2S3 nanowire network is transferred to the prepared elastic substrate and assembled with the gold electrode to obtain a flexible stretchable strain sensor; S104, performing stability test on the completed stretchable strain sensor; The information transmission unit is a Zigbee sub-node and a Zigbee master node of the host computer; The data analysis unit includes an MSP430 single-chip microcomputer, a 485 chip, a host computer and a computer; The MSP430 microcontroller, 485 chip and Zigbee sub-node are integrated on the same circuit board to form a measurement node; after collecting data, it is transmitted to the Zigbee main node of the host computer through wireless communication technology, and the host computer and computer solve and graphically process the signal.

2. The system for intelligently monitoring gastrointestinal chyme emptying signals of ruminants according to claim 1, characterized in that: The flexible stretchable strain sensor housing includes a transparent silicone hollow ring.

3. The system for intelligently monitoring gastrointestinal chyme emptying signals of ruminants according to claim 1, characterized in that: The morphology and structure of the flexible stretchable strain sensor material were characterized by field emission scanning electron microscopy and transmission electron microscopy combined with energy dispersion spectroscopy, and the stability was tested. The electrical signal and stability of the flexible stretchable strain sensor were recorded by an electrochemical workstation. The electrical signal and stability of the flexible stretchable sensor were measured in vitro by simulating the intestinal environment.

4. The system for intelligently monitoring gastrointestinal chyme emptying signals of ruminants according to claim 1, characterized in that: The flexible stretchable strain sensors are respectively fixed at the end of the abomasum and the end of the duodenum, and the collected physiological state indicators include the rest stage, the feeding stage and the rumination stage.

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