Camellia oil anti-wrinkle, anti-aging and anti-oxidation face cream and preparation method thereof

Abstract

The invention provides an anti-wrinkle, anti-aging and anti-oxidation camellia oil face cream and a preparation method thereof, and belongs to the technical field of cosmetics, the preparation method comprises the following steps: adding glycerin, 1, 3-butanediol, sorbitol, sodium hyaluronate, ethylhexylglycerin and methylparaben into water according to a formula, heating and stirring for 10-20 min, and filtering to obtain the anti-wrinkle, anti-aging and anti-oxidation camellia oil face cream. Then sequentially adding the camellia oil, the plant fermentation active matter, squalane, L-arginine, tocopherol, PEG-7 glyceryl cocoate, cetostearyl alcohol and glyceryl behenate, continuing to stir for 20-40min, then homogenizing for 4-6min, sterilizing, and discharging, so as to obtain the product. Wherein the plant fermentation active matter is prepared by carrying out crushing, gas explosion and fermentation treatment on erigeron breviscapus and plantain herb, and then carrying out heating extraction and supercritical extraction; the zymophyte is bacillus subtilis and / or lactobacillus plantarum. The face cream disclosed by the invention is stable in storage performance, mild and moist, capable of supplementing moisture and nutrition for skin in time, and excellent in anti-oxidation, anti-aging and anti-wrinkle effects.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to a camellia oil anti-wrinkle, anti-aging and anti-oxidation facial cream and a preparation method thereof. Background Art

[0002] Camellia oil, derived from the seeds of the camellia tree (Camellia japonica or Camellia olei fera), is a precious natural vegetable oil known for its rich unsaturated fatty acids (such as oleic acid and linoleic acid), camellia glycosides, vitamins (such as vitamin E), tea saponins and other ingredients. Among them, unsaturated fatty acids can penetrate the skin, provide deep moisture, help maintain the water and oil balance of the skin, and maintain the elasticity and luster of the skin. Antioxidant ingredients such as vitamins and tea saponins can resist the damage of free radicals to skin cells, reduce inflammatory reactions, and promote skin health. Active substances such as camellia glycosides help promote the regeneration of skin cells and accelerate wound healing, which is especially important for the delicate skin of babies.

[0003] Although camellia oil has many advantages mentioned above, there are still some problems that need to be improved when it is used in cosmetics such as face creams. Due to the high content of unsaturated fatty acids in camellia oil, its stability in face creams is poor, especially during long-term storage, it is prone to oxidation, causing the color of the face cream to turn yellow, thus affecting its quality and effect. In addition, high and low temperature changes will also affect the texture and stability of the face cream, resulting in a poor user experience.

[0004] In order to overcome this problem, the following methods can be used in the existing technology. For example: 1. By optimizing the product packaging design or storage environment of the cream, dark glass bottles or opaque plastic bottles are used to reduce the influence of ultraviolet rays and prevent oil oxidation; vacuum packaging, pump head or press-type bottle caps are used to reduce the chance of air contact and reduce the risk of oxidation; the product is stored in a cool place or refrigerated to avoid high temperature accelerating oil oxidation. 2. Through the formula optimization and technological innovation of the cream, a more stable emulsifier is selected or a compound emulsifier is used to enhance the emulsification stability of the cream and reduce the oil-water stratification phenomenon; camellia oil is wrapped in microcapsules to isolate external environmental factors, reduce the oxidation rate, and slowly release the effective ingredients when used; nanoemulsions are prepared using nanotechnology to improve the dispersion uniformity and stability of oils, reduce particle aggregation, and improve the stability of the overall product. The above methods can improve the stability of camellia oil cream to a certain extent, but how to further improve the comprehensive performance of the product and obtain a camellia oil cream with significant antioxidant, anti-aging and anti-wrinkle effects and stable storage performance is a problem that the present invention urgently needs to solve. Summary of the invention

[0005] The purpose of the present invention is to provide a camellia oil anti-wrinkle, anti-aging, and antioxidant facial cream, which is composed of camellia oil, plant fermentation active substances, glycerol, 1,3-butylene glycol, sorbitol, sodium hyaluronate, squalane, L-arginine, tocopherol, emulsifiers (PEG-7 glyceryl cocoate, cetearyl alcohol, glyceryl behenate), preservatives (ethylhexylglycerol, methylparaben), and water. The facial cream has excellent antioxidant, anti-aging, and anti-wrinkle effects, is mild and moisturizing, and has stable storage performance. The present invention also provides a preparation method for the camellia oil facial cream, which is simple in process and easy to operate.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] The present invention provides a camellia oil anti-wrinkle, anti-aging and anti-oxidation facial cream, comprising the following components:

[0008] Camellia oil 6-8wt%, plant fermentation active matter 1-3wt%, glycerol 6-10wt%, 1,3-butylene glycol 5-7wt%, sorbitol 5-7wt%, sodium hyaluronate 1-2wt%, squalane 1-2wt%, L-arginine 0.4-0.6wt%, tocopherol 0.1-0.3wt%, PEG-7 glyceryl cocoate 0.4-0.6wt%, cetearyl alcohol 0.8-1.2wt%, glyceryl behenate 0.8-1.2wt%, ethylhexylglycerin 0.4-0.6wt%, methylparaben 0.08-0.12wt%, and deionized water as the balance.

[0009] The facial cream of the present invention uses camellia oil as a main antioxidant and anti-wrinkle component, and tocopherol and squalane as supplements. These components provide excellent antioxidant effects, promote collagen production, and reduce fine lines and wrinkles; camellia oil also provides deep nourishment to keep the skin soft and smooth; tocopherol enhances the antioxidant effect and promotes the repair of damaged skin; squalane is similar to natural skin oil, provides lasting moisturizing and nourishing, and restores the natural oil balance of the skin; moisturizing and emollient components such as glycerol, 1,3-butylene glycol, sorbitol, sodium hyaluronate and L-arginine enhance water retention capacity, form a protective film, prevent water loss, and jointly achieve moisturizing and emollient effects; PEG-7 glyceryl cocoate, cetearyl alcohol, and glyceryl behenate serve as emulsifiers and viscosity regulators to promote stable oil-water mixing, make the facial cream smooth and easy to apply, and promote absorption; ethylhexylglycerin and methylparaben serve as preservatives to inhibit bacterial growth, extend the shelf life, ensure product safety, and are suitable for a variety of skin types, providing efficient anti-aging and moisturizing effects.

[0010] Preferably, the method for preparing the plant fermentation active substance comprises the following steps:

[0011] Step (1), taking the whole herb of Erigeron breviscapus and the whole herb of Plantago asiatica, crushing and sieving to obtain raw material powder; gas-exploding the raw material powder for 4-6 minutes to obtain pre-treated raw material powder;

[0012] Step (2), mixing the pretreated raw material powder with deionized water, sterilizing, inoculating fermentation bacteria, and fermenting at a constant temperature for 30-40 hours. After the fermentation is completed, heating and extracting for 1-3 hours, filtering, and obtaining a filtrate and a filter residue, and the filter residue is reserved; the filtrate is concentrated under reduced pressure to obtain an active component A;

[0013] Step (3), adding the filter residue obtained in step (2) into an extraction tank, supercritical extraction for 1-2 hours, to obtain an extract; filtering the extract, concentrating under reduced pressure, to obtain active component B; and mixing active component A and active component B uniformly to obtain plant fermentation active substances.

[0014] The present invention can effectively improve the stability of camellia oil by adding the plant fermentation active substance prepared by the above specific method, and inhibit the oxidation of high unsaturated acids in camellia oil into peroxides due to the action of air, ultraviolet rays, moisture and metal ions during storage, and then decompose into volatile ketone / aldehyde acid substances with peculiar smell and cause rancidity. At the same time, it can also serve as a synergistic component to further improve the antioxidant and anti-aging effects of camellia oil.

[0015] The present invention preferably uses the whole herb of Erigeron breviscapus and the whole herb of Plantago asiatica as raw materials, wherein the Erigeron breviscapus is rich in anti-oxidant and anti-inflammatory ingredients such as baicalin, tanshinone, flavonol, dihydroflavonoids, coumarins, phytosterols, caffeoyl compounds, polysaccharides, etc., and the Plantago asiatica is rich in anti-oxidant and anti-inflammatory ingredients such as rutin, quercetin, plantago glycoside, baicalin, vitamins, baicalin, triterpenes, polysaccharides, etc. Firstly, the plant raw materials are crushed and subjected to gas explosion pretreatment to make the fiber structure in the raw materials loose, which is more conducive to the subsequent fermentation treatment of fermentation bacteria and the extraction of active components, thereby ultimately improving the efficacy of plant fermentation active substances; then, Bacillus subtilis and Bacillus plantarum are used for fermentation treatment, which can not only utilize the synergistic effect of the two fermentation bacteria, Optimizing the fermentation process to produce more abundant active components can also further destroy the fiber structure of the raw materials, making the active ingredients easier to release, which is helpful for subsequent extraction and improving the extraction efficiency. Then, the heated water extraction method and the supercritical extraction method are used for combined extraction. The heated water extraction method is mainly for extracting water-soluble components, such as flavonoids, polysaccharides, etc., while the supercritical extraction method is mainly for extracting fat-soluble components, such as flavonols, phytosterols, terpenes, etc. The combination of these two extraction methods can maximize the retention of active ingredients in plant raw materials. These active ingredients can effectively inhibit the activity of unsaturated fatty acid degrading enzymes, delay oxidative rancidity, and improve the storage stability, antioxidant and anti-aging effects of camellia oil cream.

[0016] Furthermore, the method for preparing the plant fermentation active substance comprises the following steps:

[0017] Step (1), taking the washed and dried whole herb of Erigeron breviscapus and whole herb of Plantago asiatica and crushing them through a 100-300 mesh sieve to obtain raw material powder; placing the raw material powder under the conditions of 1-2 MPa and 50-65° C. for gas explosion treatment for 4-6 minutes to obtain pretreated raw material powder;

[0018] Step (2), adding the pretreated raw material powder to deionized water and mixing evenly to obtain a feed liquid, sterilizing at 115-121° C. for 15-20 min, cooling to room temperature and inoculating fermentation bacteria, and fermenting at 37-40° C. for 30-40 h. After the fermentation is completed, heating and extracting at 90-100° C. and 100-200 r / min for 1-3 h, filtering to obtain a filtrate and a filter residue, and the filter residue is reserved; the filtrate is concentrated under reduced pressure to 1-3% of the original volume to obtain an active component A;

[0019] Step (3), adding the filter residue obtained in step (2) into an extraction tank, performing supercritical extraction at 30-40Mpa and 50-60°C for 1-2h to obtain an extract; filtering the extract, and concentrating under reduced pressure to 2-4% of the original volume to obtain an active component B; and uniformly mixing the active component A and the active component B at a weight ratio of 1:1-2 to obtain a plant fermentation active substance.

[0020] Preferably, the weight ratio of the whole herb of Erigeron breviscapus to the whole herb of Plantago asiatica in step (1) is 1-3:1.

[0021] Preferably, the solid-to-liquid ratio of the pretreated raw material powder and deionized water in step (2) is 1 g:8-12 mL.

[0022] Preferably, the inoculation amount of the fermentation bacteria in step (2) is 1-2×10 8 CFU / mL feed solution.

[0023] Preferably, the fermentation bacteria in step (2) are Bacillus subtilis and / or Bacillus plantarum.

[0024] Preferably, the fermentation bacteria in step (2) are composed of Bacillus subtilis and Bacillus plantarum, and the usage ratio of Bacillus subtilis to Bacillus plantarum is 3-5:5.

[0025] Furthermore, the number of Bacillus subtilis is CI CC 22063, and the number of Bacillus plantarum is CI CC21796, both of which were purchased from the China Industrial Microbiological Culture Collection Center.

[0026] Preferably, the extraction solvent for supercritical extraction in step (3) is CO2 fluid, and the CO2 flow rate is 13-16 kg / h.

[0027] Preferably, the extraction entrainer in the supercritical extraction in step (3) is 85-95 wt % ethanol aqueous solution, and the entrainer flow rate is 0.4-0.6 g / min.

[0028] The present invention provides a method for preparing a camellia oil anti-wrinkle, anti-aging and anti-oxidation facial cream, comprising the following steps:

[0029] According to the component formula, glycerin, 1,3-butylene glycol, sorbitol, sodium hyaluronate, ethylhexylglycerin and methylparaben are added to deionized water, stirred at 50-65°C and 200-400r / min for 10-20min, and then camellia oil, plant fermentation active substances, squalane, L-arginine, tocopherol, PEG-7 glyceryl cocoate, cetearyl alcohol and glyceryl behenate are added in sequence and stirred for 20-40min. Then, homogenized at 1500-2500r / min for 4-6min, sterilized and discharged to obtain the camellia oil anti-wrinkle, anti-aging and antioxidant facial cream.

[0030] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0031] 1. The present invention provides a method for preparing a camellia oil anti-wrinkle, anti-aging and antioxidant facial cream. The camellia oil facial cream obtained by the method has stable storage performance, is mild and moisturizing, has good absorption effect, has excellent antioxidant, anti-aging and anti-wrinkle effects, can significantly improve skin elasticity and reduce wrinkles.

[0032] 2. The present invention uses camellia oil as the main antioxidant and anti-wrinkle component, tocopherol and squalane as auxiliary components, glycerin, 1,3-butylene glycol, sorbitol, sodium hyaluronate and L-arginine as moisturizing and emollient components, which enhance water retention capacity, form a protective film, prevent water loss, and jointly achieve a moisturizing and emollient effect. These ingredients provide excellent antioxidant effects, promote collagen production, reduce fine lines and wrinkles, and keep the skin soft and smooth.

[0033] 3. The present invention can effectively improve the stability of camellia oil by adding plant fermentation active substances prepared with Erigeron breviscapus and Plantain as raw materials, inhibit the oxidation and rancidity of highly unsaturated acids in camellia oil during storage, and can also serve as a synergistic component to further improve the antioxidant and anti-aging effects of camellia oil. DETAILED DESCRIPTION

[0034] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] Example 1

[0036] This embodiment provides a camellia oil anti-wrinkle, anti-aging, and anti-oxidation facial cream, comprising the following components:

[0037] Camellia oil 7.5wt%, plant fermentation active matter 2.5wt%, glycerol 8wt%, 1,3-butylene glycol 6wt%, sorbitol 6wt%, sodium hyaluronate 1.5wt%, squalane 1.5wt%, L-arginine 0.5wt%, tocopherol 0.2wt%, PEG-7 glyceryl cocoate 0.5wt%, cetearyl alcohol 1wt%, glyceryl behenate 1wt%, ethylhexylglycerin 0.5wt%, methylparaben 0.1wt%, and deionized water as the balance.

[0038] The method for preparing the plant fermentation active substance comprises the following steps:

[0039] Step (1), taking the washed and dried whole herb of Erigeron breviscapus and whole herb of Plantago asiatica and crushing them through a 200-mesh sieve to obtain a raw material powder, wherein the weight ratio of the whole herb of Erigeron breviscapus to the whole herb of Plantago asiatica is 2:1; subjecting the raw material powder to a gas explosion treatment at 1.5 MPa and 60° C. for 5 minutes to obtain a pretreated raw material powder;

[0040] Step (2), the pretreated raw material powder was added to deionized water at a solid-liquid ratio of 1g:10mL and mixed evenly to obtain a feed solution, which was sterilized at 115°C for 20min and inoculated with fermentation bacteria after cooling to room temperature. The inoculation amount of the fermentation bacteria was 1.5×10 8 CFU / mL liquid, ferment at a constant temperature of 38°C for 36 hours, and after the fermentation is completed, heat and extract at 95°C and 150r / min for 2 hours, filter to obtain a filtrate and a filter residue, and the filter residue is reserved; the filtrate is concentrated under reduced pressure to 2% of the original volume to obtain an active component A; the fermentation bacteria are composed of Bacillus subtilis and Bacillus plantarum, and the dosage ratio of Bacillus subtilis to Bacillus plantarum is 4:5;

[0041] Step (3), adding the filter residue obtained in step (2) to an extraction tank, supercritical extraction for 1.5 hours under the conditions of 35Mpa and 55°C, the extraction solvent is CO2 fluid, the CO2 flow rate is 15kg / h, the extraction entrainer is 90wt% ethanol aqueous solution, and the entrainer flow rate is 0.5g / min, to obtain an extract; filtering the extract, concentrating under reduced pressure to 3% of the original volume, to obtain active component B; mixing active component A and active component B in a weight ratio of 1:1 to obtain a plant fermentation active substance. Wherein, Bacillus subtilis is numbered as CI CC 22063, and Bacillus plantarum is numbered as CI CC 21796, both of which are purchased from the China Industrial Microbial Culture Collection Management Center.

[0042] This embodiment provides a method for preparing a camellia oil anti-wrinkle, anti-aging and anti-oxidation cream, comprising the following steps:

[0043] According to the component formula, glycerin, 1,3-butylene glycol, sorbitol, sodium hyaluronate, ethylhexylglycerin and methylparaben are added to deionized water, stirred at 60°C and 300 r / min for 15 minutes, and then camellia oil, plant fermentation active substances, squalane, L-arginine, tocopherol, PEG-7 glyceryl cocoate, cetearyl alcohol and glyceryl behenate are added in sequence and stirred for 30 minutes. Then, homogenized at 2000 r / min for 5 minutes, sterilized and discharged to obtain the camellia oil anti-wrinkle, anti-aging and antioxidant facial cream.

[0044] Example 2

[0045] A camellia oil anti-wrinkle, anti-aging and anti-oxidation facial cream, comprising the following components:

[0046] Camellia oil 6wt%, plant fermentation active matter 1wt%, glycerol 6wt%, 1,3-butylene glycol 5wt%, sorbitol 5wt%, sodium hyaluronate 1wt%, squalane 1wt%, L-arginine 0.4wt%, tocopherol 0.1wt%, PEG-7 glyceryl cocoate 0.4wt%, cetearyl alcohol 0.8wt%, glyceryl behenate 0.8wt%, ethylhexylglycerin 0.4wt%, methylparaben 0.08wt%, and deionized water as the balance.

[0047] The preparation method of the plant fermentation active substance is the same as that in Example 1.

[0048] This embodiment provides a method for preparing a camellia oil anti-wrinkle, anti-aging and anti-oxidation cream, comprising the following steps:

[0049] According to the component formula, glycerin, 1,3-butylene glycol, sorbitol, sodium hyaluronate, ethylhexylglycerin and methylparaben are added to deionized water, and stirred at 50°C and 200 r / min for 10 minutes. Camellia oil, plant fermentation active substances, squalane, L-arginine, tocopherol, PEG-7 glyceryl cocoate, cetearyl alcohol and glyceryl behenate are added in sequence and stirred for 20 minutes. Then, homogenize at 1500 r / min for 4 minutes, sterilize and discharge to obtain the camellia oil anti-wrinkle, anti-aging and antioxidant facial cream.

[0050] Example 3

[0051] A camellia oil anti-wrinkle, anti-aging and anti-oxidation facial cream, comprising the following components:

[0052] Camellia oil 8wt%, plant fermentation active matter 3wt%, glycerol 10wt%, 1,3-butylene glycol 7wt%, sorbitol 7wt%, sodium hyaluronate 2wt%, squalane 2wt%, L-arginine 0.6wt%, tocopherol 0.3wt%, PEG-7 glyceryl cocoate 0.6wt%, cetearyl alcohol 1.2wt%, glyceryl behenate 1.2wt%, ethylhexylglycerin 0.6wt%, methylparaben 0.12wt%, and deionized water as the balance.

[0053] The preparation method of the plant fermentation active substance is the same as that in Example 1.

[0054] This embodiment provides a method for preparing a camellia oil anti-wrinkle, anti-aging and anti-oxidation cream, comprising the following steps:

[0055] According to the component formula, glycerin, 1,3-butylene glycol, sorbitol, sodium hyaluronate, ethylhexylglycerin and methylparaben are added to deionized water, stirred at 65°C and 400 r / min for 20 min, and then camellia oil, plant fermentation active substances, squalane, L-arginine, tocopherol, PEG-7 glyceryl cocoate, cetearyl alcohol and glyceryl behenate are added in sequence and stirred for 40 min. Then, homogenized at 2500 r / min for 6 min, sterilized and discharged to obtain the camellia oil anti-wrinkle, anti-aging and antioxidant facial cream.

[0056] Comparative Example 1

[0057] The difference between this comparative example and Example 1 is that the step (1) in the preparation method of the plant fermentation active substance is different, which is as follows: the cleaned and dried whole herb of Erigeron breviscapus is crushed through a 200-mesh sieve to obtain a raw material powder; the raw material powder is subjected to gas explosion treatment at 1.5 MPa and 60° C. for 5 minutes to obtain a pretreated raw material powder.

[0058] Comparative Example 2

[0059] The difference between this comparative example and Example 1 is that the step (1) in the preparation method of the plant fermentation active substance is different, which is as follows: the whole plant grass that has been washed and dried is crushed through a 200-mesh sieve to obtain a raw material powder; the raw material powder is subjected to gas explosion treatment at 1.5 MPa and 60° C. for 5 minutes to obtain a pretreated raw material powder.

[0060] Comparative Example 3

[0061] The difference between this comparative example and Example 1 is that the step (1) in the preparation method of the plant fermentation active substance is different, which is as follows: the cleaned and dried whole herb of Erigeron breviscapus and whole herb of Plantago asiatica are taken and crushed through a 200-mesh sieve, and the weight ratio of the whole herb of Erigeron breviscapus to the whole herb of Plantago asiatica is 2:1; and the pretreated raw material powder is obtained.

[0062] Comparative Example 4

[0063] The difference between this comparative example and Example 1 is that the step (2) in the preparation method of the plant fermentation active substance is different, which is as follows: the pretreated raw material powder is added to deionized water at a solid-liquid ratio of 1g:10mL and mixed evenly, heated and extracted at 95°C and 150r / min for 2h, filtered to obtain a filtrate and a filter residue, and the filter residue is reserved; the filtrate is concentrated under reduced pressure to 2% of the original volume to obtain active component A.

[0064] Comparative Example 5

[0065] The difference between this comparative example and Example 1 is that the step (2) in the preparation method of the plant fermentation active substance is different, which is as follows: the pretreated raw material powder is added to deionized water at a solid-liquid ratio of 1g:10mL and mixed evenly to obtain a feed liquid, which is sterilized at 115°C for 20min and inoculated with fermentation bacteria after cooling to room temperature. The inoculation amount of the fermentation bacteria is 1.5×10 8 CFU / mL liquid, ferment at a constant temperature of 38°C for 36 hours, and after the fermentation, heat and extract at 95°C and 150r / min for 2 hours, filter to obtain a filtrate and a residue, and use the residue for later use; the filtrate is concentrated under reduced pressure to 2% of the original volume to obtain active component A; the fermentation bacteria is Bacillus subtilis.

[0066] Comparative Example 6

[0067] The difference between this comparative example and Example 1 is that the step (2) in the preparation method of the plant fermentation active substance is different, which is as follows: the pretreated raw material powder is added to deionized water at a solid-liquid ratio of 1g:10mL and mixed evenly to obtain a feed liquid, which is sterilized at 115°C for 20min and inoculated with fermentation bacteria after cooling to room temperature. The inoculation amount of the fermentation bacteria is 1.5×10 8 CFU / mL liquid, ferment at a constant temperature of 38°C for 36 hours, and after the fermentation is completed, heat and extract at 95°C and 150r / min for 2 hours, filter to obtain a filtrate and a residue, and use the residue for later use; the filtrate is decompressed and concentrated to 2% of the original volume to obtain active component A; the fermentation bacteria is plant lactobacillus.

[0068] Comparative Example 7

[0069] The difference between this comparative example and Example 1 is that the preparation method of the plant fermentation active substance is different, as follows:

[0070] Step (1), taking the washed and dried whole herb of Erigeron breviscapus and whole herb of Plantago asiatica and crushing them through a 200-mesh sieve to obtain a raw material powder, wherein the weight ratio of the whole herb of Erigeron breviscapus to the whole herb of Plantago asiatica is 2:1; subjecting the raw material powder to a gas explosion treatment at 1.5 MPa and 60° C. for 5 minutes to obtain a pretreated raw material powder;

[0071] Step (2), the pretreated raw material powder was added to deionized water at a solid-liquid ratio of 1g:10mL and mixed evenly to obtain a feed solution, which was sterilized at 115°C for 20min and inoculated with fermentation bacteria after cooling to room temperature. The inoculation amount of the fermentation bacteria was 1.5×10 8 CFU / mL liquid, ferment at a constant temperature of 38°C for 36 hours, after the fermentation is completed, heat and extract at 95°C and 150r / min for 2 hours, filter to obtain a filtrate and a filter residue, and reduce the pressure to concentrate the filtrate to 2% of the original volume. The fermentation bacteria are composed of Bacillus subtilis and Bacillus plantarum, and the dosage ratio of Bacillus subtilis to Bacillus plantarum is 4:5 to obtain plant fermentation active substances.

[0072] Performance Testing

[0073] 1. Stability measurement: The camellia oil anti-wrinkle, anti-aging and anti-oxidation creams prepared in the above Examples 1-3 and Comparative Examples 1-7 were placed under the following storage conditions for a period of time to observe whether the creams changed color, separated or produced any odor. If there was no color change, no separation or no odor, it was recorded as qualified; if at least one of the following appeared, it was recorded as unqualified. The specific storage conditions were as follows: 4°C for 2 months, 25°C for 2 months, and 55°C for 2 months. The results are shown in Table 1.

[0074] Table 1: Stability test results

[0075]

[0076]

[0077] 2. Anti-wrinkle and anti-aging determination: 150 female volunteers with healthy skin (aged 35-45 years old) were invited and randomly divided into 10 groups, 15 in each group. Each group of subjects corresponded to the camellia oil anti-wrinkle, anti-aging and antioxidant creams prepared in the above Examples 1-3 and Comparative Examples 1-7 as samples. The experimental period was 2 months. During this period, the subjects applied the samples on the left half of their faces, and did not apply any cosmetics on the right half of their faces as a blank control. The samples were used once in the morning and evening. At the beginning and end of the experiment, the skin of the subjects was tested and analyzed using a skin detector to evaluate the improvement effect of the above cream on the skin elasticity of the cheeks and fine lines at the corners of the eyes (+ for increase, - for decrease), and the average value was taken. The results are shown in Table 2.

[0078] Table 2: Anti-wrinkle and anti-aging test results

[0079]

[0080]

[0081] It can be seen from the above performance test results that the camellia oil cream prepared in Examples 1-3 has stable storage performance (high and low temperature), can effectively improve skin elasticity, fade fine lines, and has good anti-wrinkle and anti-aging effects. In particular, the comprehensive performance of the camellia oil cream in Example 3 is the most outstanding. This is because the present invention can effectively improve the stability of camellia oil by adding plant fermentation active substances prepared with Erigeron breviscapus and Plantain as raw materials, and inhibit the oxidation of highly unsaturated acids in camellia oil into peroxides due to the action of air, ultraviolet rays, moisture and metal ions during storage, and then decompose into volatile, odorous ketone / aldehyde acid substances to cause rancidity. At the same time, it can also serve as a synergistic component to further improve the antioxidant and anti-aging effects of camellia oil.

[0082] In comparison, since Comparative Examples 1-7 did not adopt the necessary technical solutions, they were significantly worse than Examples 1-3 in the corresponding performance tests. In Comparative Examples 1-3, the preferred raw material combination and pretreatment steps were not adopted in the preparation of plant fermentation actives, and specific fermentation bacteria were not used for fermentation treatment in Comparative Examples 4-6. It can be seen from the results that this led to a decrease in the stability and anti-aging effect of the camellia oil cream. The above experimental results further prove the importance of the technical solution defined in the present invention for its technical effects.

[0083] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A camellia oil anti-wrinkle, anti-aging and antioxidant facial cream, characterized in that: It consists of the following components: Camellia oil 6-8wt%, plant fermentation active matter 1-3wt%, glycerol 6-10wt%, 1,3-butylene glycol 5-7wt%, sorbitol 5-7wt%, sodium hyaluronate 1-2wt%, squalane 1-2wt%, L-arginine 0.4-0.6wt%, tocopherol 0.1-0.3wt%, PEG-7 glyceryl cocoate 0.4-0.6wt%, cetearyl alcohol 0.8-1.2wt%, glyceryl behenate 0.8-1.2wt%, ethylhexylglycerin 0.4-0.6wt%, methylparaben 0.08-0.12wt%, water as the balance; The plant fermentation active substance is prepared by crushing, gas-exploding and fermenting breviscapus and plantain, and then performing heating extraction and supercritical extraction.

2. The camellia oil anti-wrinkle, anti-aging and antioxidant facial cream according to claim 1, characterized in that: The method for preparing the plant fermentation active substance comprises the following steps: Step (1), taking the whole herb of Erigeron breviscapus and the whole herb of Plantago asiatica, crushing and sieving to obtain raw material powder; gas-exploding the raw material powder for 4-6 minutes to obtain pre-treated raw material powder; Step (2), mixing the pretreated raw material powder with water, sterilizing, inoculating fermentation bacteria, and fermenting at a constant temperature for 30-40 hours. After the fermentation is completed, heating and extracting for 1-3 hours, filtering, and obtaining a filtrate and a filter residue, and the filter residue is reserved; the filtrate is concentrated under reduced pressure to obtain an active component A; Step (3), adding the filter residue obtained in step (2) into an extraction tank, supercritical extraction for 1-2 hours, to obtain an extract; filtering the extract, concentrating under reduced pressure, to obtain active component B; and mixing active component A and active component B uniformly to obtain plant fermentation active substances.

3. The camellia oil anti-wrinkle, anti-aging and antioxidant facial cream according to claim 2, characterized in that: The method for preparing the plant fermentation active substance comprises the following steps: Step (1), taking the washed and dried whole herb of Erigeron breviscapus and whole herb of Plantago asiatica and crushing them through a 100-300 mesh sieve to obtain raw material powder; placing the raw material powder under the conditions of 1-2 MPa and 50-65° C. for gas explosion treatment for 4-6 minutes to obtain pretreated raw material powder; Step (2), adding the pretreated raw material powder to water and mixing evenly to obtain a feed liquid, sterilizing at 115-121° C. for 15-20 min, cooling to room temperature and inoculating fermentation bacteria, and fermenting at 37-40° C. for 30-40 h. After the fermentation is completed, heating and extracting at 90-100° C. and 100-200 r / min for 1-3 h, filtering to obtain a filtrate and a filter residue, and the filter residue is reserved; the filtrate is concentrated under reduced pressure to 1-3% of the original volume to obtain an active component A; Step (3), adding the filter residue obtained in step (2) into an extraction tank, performing supercritical extraction at 30-40Mpa and 50-60°C for 1-2h to obtain an extract; filtering the extract, and concentrating under reduced pressure to 2-4% of the original volume to obtain an active component B; and uniformly mixing the active component A and the active component B at a weight ratio of 1:1-2 to obtain a plant fermentation active substance.

4. The camellia oil anti-wrinkle, anti-aging and antioxidant facial cream according to claim 3, characterized in that: The weight ratio of the whole herb of Erigeron breviscapus to the whole herb of Plantago asiatica in step (1) is 1-3:

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5. The camellia oil anti-wrinkle, anti-aging and anti-oxidation cream according to claim 3, characterized in that: The fermentation bacteria in step (2) are Bacillus subtilis and / or Bacillus plantarum.

6. The camellia oil anti-wrinkle, anti-aging and anti-oxidation facial cream according to claim 3, characterized in that: The inoculation amount of the fermentation bacteria in step (2) is 1-2×10 8 CFU / mL feed solution.

7. The camellia oil anti-wrinkle, anti-aging and anti-oxidation cream according to claim 3, characterized in that: The solid-to-liquid ratio of the pretreated raw material powder and water in step (2) is 1 g:8-12 mL.

8. The camellia oil anti-wrinkle, anti-aging and anti-oxidation cream according to claim 3, characterized in that: The extraction solvent of the supercritical extraction in step (3) is CO2 fluid, and the CO2 flow rate is 13-16kg / h.

9. The camellia oil anti-wrinkle, anti-aging and anti-oxidation cream according to claim 3, characterized in that: In step (3), the extraction entrainer of the supercritical extraction is 85-95wt% ethanol aqueous solution, and the entrainer flow rate is 0.4-0.6g / min.

10. The method for preparing the camellia oil anti-wrinkle, anti-aging and anti-oxidation cream according to any one of claims 1 to 9, characterized in that: The steps include: According to the component formula, glycerin, 1,3-butylene glycol, sorbitol, sodium hyaluronate, ethylhexylglycerin and methylparaben are added to water, stirred at 50-65°C and 200-400r / min for 10-20min, then camellia oil, plant fermentation active substances, squalane, L-arginine, tocopherol, PEG-7 glyceryl cocoate, cetearyl alcohol and glyceryl behenate are added in sequence and stirred for 20-40min, then homogenized at 1500-2500r / min for 4-6min, sterilized and discharged to obtain the camellia oil anti-wrinkle, anti-aging and antioxidant facial cream.

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