Electrochemiluminescence immunodetector

Abstract

The invention relates to an electrochemical luminescence immunoassay detector. The electrochemical luminescence immunodetector comprises a shell, and an incubation module, a magnetic separation module, a detection module, a lofting module, a reagent module, a consumable module, a first conveying mechanism, a second conveying mechanism and a third conveying mechanism which are assembled in the shell. The incubation module has a first direction and a second direction which are perpendicular to each other, in the first direction, the magnetic separation module and the detection module are both arranged on one side of the incubation module, the lofting module is arranged on the other side of the incubation module, and in the second direction, the reagent module and the consumable module are located on the two sides of the incubation module respectively. In the second direction, the first conveying mechanism is arranged on the side, away from the consumable module, of the incubation module, and a first swing arm of the first conveying mechanism is configured to convey substances in the sample placing module to an incubation rotary disc of the incubation module. And the second conveying mechanism is arranged between the incubation module and the reagent module. And the third conveying mechanism is arranged between the magnetic separation module and the detection module.

Description

Technical Field

[0001] The present application relates to the field of medical detection technology, and in particular to an electrochemiluminescence immunoassay instrument. Background Art

[0002] The electrochemiluminescence immunoassay is an important medical instrument that combines the principles of electrochemistry and chemiluminescence and is used for quantitative analysis of various hormones, tumor markers, etc. The electrochemiluminescence immunoassay is based on the electrochemiluminescence immunoassay (ECLIA). The electrochemiluminescence immunoassay is based on the principle of light emission in a chemical environment to detect and analyze different proteins and biomolecules. The antigen or antibody is labeled with the chemiluminescent agent terpyridine ruthenium [Ru(byp)2+3], and tripropylamine (TPA) is used as an electron donor. Through the electron transfer in the electric field, it produces chemiluminescence.

[0003] In the related art, the electrochemiluminescence immunoassay detector has a large overall size and low detection efficiency. Summary of the invention

[0004] Based on this, it is necessary to provide an electrochemiluminescence immunoassay instrument to address the problems of large overall size and low detection speed of the electrochemiluminescence immunoassay instrument.

[0005] An electrochemiluminescence immunoassay instrument comprises:

[0006] case;

[0007] and an incubation module, a magnetic separation module, a detection module, a sample placement module, a reagent module, a consumables module, a first conveying mechanism, a second conveying mechanism and a third conveying mechanism assembled in the housing;

[0008] The incubation module has a first direction and a second direction that are perpendicular to each other. In the first direction, the magnetic separation module and the detection module are both arranged on one side of the incubation module, and the placement module is arranged on the other side of the incubation module. In the second direction, the reagent module and the consumables module are respectively located on both sides of the incubation module.

[0009] In the second direction, the first conveying mechanism is arranged on a side of the incubation module away from the consumables module, and the first swing arm of the first conveying mechanism is configured to convey the material in the placement module to the incubation turntable of the incubation module;

[0010] The second conveying mechanism is arranged between the incubation module and the reagent module, and the second swing arm of the second conveying mechanism is configured to convey at least one substance in the reagent module to the incubation turntable;

[0011] The magnetic separation module is configured to perform magnetic separation processing on the substances placed on the incubation turntable;

[0012] The third transport mechanism is arranged between the magnetic separation module and the detection module, and is configured to transport the substances that have undergone magnetic separation treatment in the incubation turntable to the detection module.

[0013] In one embodiment, the incubation module further includes a first needle cleaning member and / or a second needle cleaning member and / or a third needle cleaning member and / or a first mixing member;

[0014] Among them, the first needle cleaning part and the third needle cleaning part are both arranged in the circular arc trajectory of the first needle assembled on the first swing arm, the second needle cleaning part is arranged in the circular arc trajectory of the second needle assembled on the second swing arm, and the first mixing part is arranged between the incubation turntable and the consumable module in the second direction.

[0015] In one embodiment, the magnetic separation module includes a first clamping mechanism, a liquid extraction / injection mechanism, a magnetic separation component, a cleaning / liquid storage component, and a second mixing component;

[0016] The magnetic separation module has a horizontal track line parallel to the second direction, the first clamping mechanism and the liquid extraction / injection mechanism are translated along the horizontal track line, and the incubation turntable, the magnetic separation element, the cleaning / liquid storage element and the second mixing element are all located within the horizontal track line;

[0017] The first clamping mechanism is configured to place the consumables on the incubation turntable, the magnetic separation element, and the second mixing element;

[0018] The magnetic separator is provided with a cylindrical magnetic part, and a placement space is formed in the magnetic part, and the placement space is used to place consumables;

[0019] The liquid extraction needle of the liquid extraction / injection mechanism is configured to extract liquid from the consumable, and the liquid injection needle of the liquid extraction / injection mechanism is configured to inject liquid into the consumable;

[0020] The cleaning / liquid storage part includes a fourth needle cleaning part and a liquid storage part. The fourth needle cleaning part is configured to clean the liquid extraction needle, and the liquid storage part is used to store the liquid to be extracted by the liquid extraction needle.

[0021] In one embodiment, the sample placement module is provided with a sample injection channel, a collection channel and a sample output channel, the collection channel is connected to the sample injection channel and the sample output channel, and the extension direction of the collection channel is perpendicular to the extension direction of the sample injection channel and the extension direction of the sample output channel;

[0022] The sample placement module also includes a first driving component, which is configured to drive the sample loading component to move along the sample injection channel, the collection channel and the sample output channel in sequence.

[0023] In one embodiment, the sample placement module further comprises a sample placement module housing, the sample placement module housing is provided with a separation boss, and the separation boss defines a sample inlet channel, a collection channel and a sample outlet channel in the sample placement module housing;

[0024] The separation boss is provided with a spare sample loading space, and the spare sample loading space is arranged in the arc track line of the first needle head assembled on the first swing arm.

[0025] In one embodiment, the first driving assembly includes a first pushing member and a second pushing member, wherein the pushing claw of the first pushing member is configured to push the sample loading member in the sample injection channel to the collection channel, and the pushing claw of the second pushing member is configured to push the sample loading member in the collection channel to the sample output channel;

[0026] The first driving assembly includes a third pushing member, and the third pushing member is configured to drive the sample loading member to move from the sample inlet channel to the sample outlet channel along an extension direction in the collection channel.

[0027] In one embodiment, the reagent module includes a third drive component and a reagent turntable, the reagent turntable is assembled in the assembly space of the third drive component, the reagent turntable is assembled in the assembly space, and the third drive component drives the reagent turntable to rotate, and the reagent turntable is used to place at least one reagent group.

[0028] In one embodiment, the third driving assembly includes an assembly housing and a first driver, the first driver is assembled in the assembly housing, and the first driver drives the reagent turntable to rotate;

[0029] An assembly space is formed in the assembly housing, and a driving rack is also provided in the assembly space. The driving rack is extended along the circumference of the assembly space, and the driving rack is meshed with a meshing gear provided on the reagent turntable for transmission. The meshing gear rotates to drive a reagent tube in the reagent group to rotate;

[0030] The reagent module also includes a temperature regulating member, which is fitted with the assembly shell and is used to adjust the temperature value in the assembly space.

[0031] In one embodiment, the consumable module includes a consumable module base plate, a stage, and a third clamping mechanism;

[0032] Among them, the consumable module bottom plate has a vertical third direction and a fourth direction, the third direction is parallel to the first direction, and the fourth direction is parallel to the second direction; the worktable is slidably assembled on the consumable module bottom plate along the fourth direction, and the worktable is used to place consumables; the third clamping mechanism includes a fourth drive assembly and a clamp, the fourth drive assembly drives the clamp to move along the third direction and the fourth direction, and the clamp is configured to take and place the consumables on the worktable and the incubation module.

[0033] In one of the embodiments, the fourth drive assembly includes a sliding gantry and a second driver, the second driver is mounted on the sliding gantry, and the second driver is configured to drive the third clamping mechanism to move along the third direction;

[0034] The sliding gantry is slidably assembled with the consumable module bottom plate along the fourth direction, and the sliding gantry and the consumable module bottom plate together define a through space that passes through along the fourth direction, and the loading platform is suitable for passing through the through space.

[0035] The various components in the electrochemiluminescence immunoassay are reasonably and compactly arranged, achieving the effect of high integration and miniaturization of the electrochemiluminescence immunoassay, and the total detection time of the electrochemiluminescence immunoassay is short, with the advantage of high detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 Schematic diagram of the structure of an electrochemiluminescence immunoassay instrument according to one embodiment of the present application.

[0037] Figure 2 for Figure 1 Enlarged view of point A in .

[0038] Figure 3 It is a schematic diagram of the structure of an electrochemiluminescence immunoassay instrument according to another embodiment of the present application.

[0039] Figure 4 for Figure 3 Enlarged view of point B in .

[0040] Figure 5 for Figure 3 Enlarged view of point C in the figure.

[0041] Figure 6 Schematic diagram of the structure of an electrochemiluminescence immunoassay instrument according to another embodiment of the present application.

[0042] Figure 7 Schematic diagram of the structure of an incubation module according to one embodiment of the present application.

[0043] Figure 8 Schematic diagram of the structure of a magnetic separation module according to an embodiment of the present application.

[0044] Fig. 9 Schematic diagram of the structure of a third driving assembly according to an embodiment of the present application.

[0045] Fig.10 Schematic diagram of the structure of a reagent turntable according to an embodiment of the present application.

[0046] Fig.11 FIG. 4 is a bottom view of a reagent module according to an embodiment of the present application.

[0047] Fig.12 Schematic diagram of the structure of a consumable module according to an embodiment of the present application.

[0048] Fig.13 Schematic diagram of the structure of a third conveying mechanism according to an embodiment of the present application.

[0049] Figure Number:

[0050] 100. Electrochemiluminescence immunoassay instrument; 1. Housing; 2. Incubation module; 21. Incubation turntable; 22. First needle cleaning member; 23. Second needle cleaning member; 24. Third needle cleaning member; 25. First mixing member; 3. Magnetic separation module; 31. First clamping mechanism; 32. Extraction / injection mechanism; 321. Extraction needle; 322. Injection needle; 33. Magnetic separation member; 34. Cleaning / liquid storage member; 341. Fourth needle cleaning member; 342. Liquid storage member Component; 35, second mixing component; 4, detection module; 5, sample placement module; 50a, sample injection channel; 50b, collection channel; 50c, sample outlet channel; 51, sample placement module housing; 52, partition boss; 520, spare sample loading space; 53a, first pusher; 53b, second pusher; 53c, third pusher; 6, reagent module; 61, third drive assembly; 611, assembly housing; 6110, assembly space; 611a, shell wall component; 611 b, bottom plate; 611c, drive rack; 612, first driver; 612a, drive motor; 612b, drive belt; 612c, drive disk; 62, reagent turntable; 621, meshing gear; 63, temperature adjustment member; 7, consumable module; 71, consumable module bottom plate; 72, stage; 73, third clamping mechanism; 74, through space; 731, fourth drive assembly; 731a, sliding gantry; 731b, second driver; 732 , clamp; 81, first conveying mechanism; 810, first swing arm; 811, first needle; 812, first swing arm driver; 82, second conveying mechanism; 820, second swing arm; 821, second needle; 822, second swing arm driver; 83, third conveying mechanism; 831, third needle; 832, fifth needle cleaning part; 833, detection reagent storage unit; S1, first circular arc trajectory; S2, second circular arc trajectory; S3, horizontal trajectory. DETAILED DESCRIPTION

[0051] In order to make the above-mentioned purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are described in detail below in conjunction with the accompanying drawings. In the following description, many specific details are set forth to facilitate a full understanding of the present application. However, the present application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present application, so the present application is not limited by the specific embodiments disclosed below.

[0052] In the description of the present application, it should be understood that if the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. appear, the orientation or position relationship indicated by these terms is based on the orientation or position relationship shown in the accompanying drawings, which is only for the convenience of describing the present application and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present application.

[0053] In addition, if the terms "first" or "second" appear, these terms are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the features. In the description of this application, if the term "plurality" appears, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0054] In this application, unless otherwise clearly specified and limited, if the terms "installed", "connected", "connected", "fixed" and the like appear, these terms should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integrated connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to the specific circumstances.

[0055] In the present application, unless otherwise clearly specified and limited, if there is a description that a first feature is "above" or "below" a second feature, etc., or similar descriptions appear, it may mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature being "above", "above" and "above" the second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. The first feature being "below", "below" and "below" the second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0056] It should be noted that if an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be a central element. If an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. If any, the terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used in this application are for illustrative purposes only and do not represent the only implementation method.

[0057] See also Figures 1 to 6 As shown, the electrochemiluminescence immunoassay instrument 100 according to some embodiments of the present application includes a housing 1, an incubation module 2, a magnetic separation module 3, a detection module 4, a sample placement module 5, a reagent module 6, a consumable module 7, a first conveying mechanism 81, a second conveying mechanism 82 and a third conveying mechanism 83, and the incubation module 2, the magnetic separation module 3, the detection module 4, the sample placement module 5, the reagent module 6, the consumable module 7, the first conveying mechanism 81, the second conveying mechanism 82 and the third conveying mechanism 83 are all assembled in the housing 1. It can also be understood that the electrochemiluminescence immunoassay instrument 100 is integrated with the incubation module 2, the magnetic separation module 3, the detection module 4, the sample placement module 5, the reagent module 6, the consumable module 7, the first conveying mechanism 81, the second conveying mechanism 82 and the third conveying mechanism 83, so that the electrochemiluminescence immunoassay instrument 100 can meet the needs of qualitative or quantitative detection of analytes in human serum and plasma samples, such as any one of the tumor marker detection items, thyroid detection items, myocardial cardiopulmonary infection items, sex hormone detection items, etc.

[0058] Combination Figures 1 to 3 As shown, the incubation module 2 has a first direction and a second direction that are perpendicular to each other. Figure 1 As shown, the first direction is Figure 1 The Y direction shown in Figure 1 The X direction shown in .

[0059] Combination Figure 1 and Figure 2 As shown, in the first direction (i.e., the Y direction), the magnetic separation module 3 and the detection module 4 are both arranged on one side of the incubation module 2, and the sample placement module 5 is arranged on the other side of the incubation module 2. In the second direction (i.e., the X direction), the reagent module 6 and the consumables module 7 are respectively located on both sides of the incubation module 2, so that the magnetic separation module 3, the detection module 4, the sample placement module 5, the reagent module 6, and the consumables module 7 are arranged in the circumferential direction of the incubation module 2. For example, see Figure 2 As shown, in one embodiment of the present application, along the circumference of the incubation module 2, the magnetic separation module 3, the detection module 4, the consumable module 7, the placement module 5 and the reagent module 6 are sequentially arranged in a clockwise manner.

[0060] For example, see Figure 2 As shown, in one embodiment, the first conveying mechanism 81 may include a first swing arm 810 and a first swing arm driver 812. Along the length direction of the first swing arm 810, the first swing arm 810 has a fixed end and a free end relative to each other. The fixed end of the first swing arm 810 is assembled with the first swing arm driver 812. The first swing arm driver 812 drives the first swing arm 810 to swing, so that the free end of the first swing arm 810 moves in a circular arc. In addition, the first swing arm driver 812 can also drive the first swing arm 810 to move up and down. The first swing arm driver 812 drives the first swing arm 810 to move, so that the first swing arm 810 can be used to transport the material in the sample module 5 to the incubation turntable 21 of the incubation module 2. It should be noted that, in one embodiment, the substance placed in the sample placement module 5 may be a human sample collected from the human body, and the electrochemiluminescence immunoassay instrument 100 is used to analyze and process the human sample to obtain corresponding information. In order to facilitate the understanding of the present application, in the following content, the substance placed in the sample placement module 5 is taken as a human sample as an example for explanation, but the present application is not limited to this. For example, the substance placed in the sample placement module 5 may also be a sample collected from animals such as dogs and cats.

[0061] For example, see Figure 2 As shown, in one embodiment, the second conveying mechanism 82 may include a second swing arm 820 and a second swing arm driver 822. Along the length direction of the second swing arm 820, the second swing arm 820 has a fixed end and a free end relative to each other. The fixed end of the second swing arm 820 is assembled with the second swing arm driver 822. The second swing arm driver 822 drives the second swing arm 820 to swing, so that the free end of the second swing arm 820 moves in a circular arc. In addition, the second swing arm driver 822 can also drive the second swing arm 820 to move up and down. The second swing arm driver 822 drives the second swing arm 820 to move, so that the second swing arm 820 can be used to transport the substances in the reagent module 6 to the incubation turntable 21 of the incubation module 2.

[0062] The second swing arm driver 822 is arranged between the incubation module 2 and the reagent module 6, so that the second swing arm 820 can be used to transport at least one substance placed in the reagent module 6 to the incubation turntable 21. It should be noted that the reagent module 6 is used to place reagents for reacting with human samples, so that the electrochemiluminescence immunoassay instrument 100 can analyze and process human samples to obtain corresponding information.

[0063] Exemplarily, in some embodiments of the present application, at least two reagent groups are placed in the reagent module 6, wherein each reagent group may include three reagents, the three reagents include a first reagent, a second reagent and a magnetic bead reagent, and it should be noted that the magnetic bead reagent contains magnetic beads. The three reagents can be transported from the reagent module 6 to a test tube containing a human sample placed in the incubation turntable 21 by the second swing arm 820, so that the three reagents react with the human sample to form a mixed substance, the mixed substance includes a substance to be tested and a mixed liquid, the substance to be tested is used for detection in subsequent steps, and the substance to be tested contains magnetic beads.

[0064] See also Figure 2 As shown, the partial structure of the magnetic separation module 3 can move the test tube containing the mixed substance from the incubation turntable 21 to the magnetic separation module 3, so that the mixed substance is further processed in the magnetic separation module 3 to form the mixed substance to be tested. Then, the partial structure of the magnetic separation module 3 moves the test tube containing the mixed substance to be tested from the magnetic separation module 3 to the incubation turntable 21, so that the third conveying mechanism 83 arranged between the magnetic separation module 3 and the detection module 4 extracts the mixed substance to be tested and conveys it to the detection module 4.

[0065] Exemplarily, in one embodiment of the present application, the operator places the first test tube containing the human sample in the sample placement module 5, and the first swing arm driver 812 drives the first swing arm 810 to move, so that the first swing arm 810 can extract the human sample from the first test tube containing the human sample. After the first swing arm 810 completes the extraction, the first swing arm driver 812 drives the first swing arm 810 to move toward the incubation turntable 21, and the first swing arm 810 transports the human sample to the second test tube placed on the incubation turntable 21. It should be supplemented that the second test tube is stored in the consumable module 7, and a part of the structure of the consumable module 7 transports the second test tube to the incubation turntable 21, so that the second test tube can be used to receive the human sample transported by the first swing arm 810.

[0066] In addition, the first swing arm 820 of the second conveying mechanism 82 extracts the first reagent, the second reagent and the magnetic bead reagent from the reagent module 6 in sequence, and conveys the first reagent, the second reagent and the magnetic bead reagent to the second test tube containing the human body sample in sequence, so that the human body sample and the first reagent, the second reagent, and the magnetic bead reagent are fused in the second test tube to form a mixed substance. After the human body sample and the first reagent, the second reagent, and the magnetic bead reagent are fused in the second test tube to form a mixed substance, the partial structure of the magnetic separation module 3 moves the second test tube containing the mixed substance to the magnetic separation module 3, so that the mixed substance is further processed in the magnetic separation module 3 to form a mixed substance to be tested. After the mixed substance to be tested is formed, the partial structure of the magnetic separation module 3 moves the second test tube containing the mixed substance to be tested to the incubation turntable 21. Then the third conveying mechanism 83 extracts the mixed substance to be tested and conveys it to the detection module 4, so that the detection module 4 detects the mixed substance to be tested.

[0067] It is worth noting that, since the incubation module 2 is provided with an incubation turntable 21 that can rotate, during the detection process of the electrochemiluminescence immunoassay instrument 100, after the first swing arm 810 transports the human sample in the sample module 5 to the second test tube placed in the incubation turntable 21, the incubation turntable 21 rotates clockwise to move the second test tube to a position that is convenient for the second swing arm 820 to transport the three reagents in the reagent module 6 to the second test tube, so that the human sample and the first reagent, the second reagent, and the magnetic bead reagent are fused and reacted in the second test tube to form a mixed substance. Then the incubation turntable 21 continues to rotate clockwise to move the second test tube to a position that is convenient for the magnetic separation module 3 to contact the second test tube containing the mixed substance, so that the magnetic separation module 3 can move the second test tube containing the mixed substance from the incubation turntable 21 to the magnetic separation module 3, thereby realizing that the magnetic separation module 3 processes the mixed substance to form the mixed substance to be tested. Then the magnetic separation module 3 moves the second test tube with the mixed substance to be tested from the magnetic separation module 3 to the incubation turntable 21, and then the incubation turntable 21 continues to rotate clockwise, so that the third conveying mechanism 83 can extract the mixed substance to be tested from the second test tube, and then the mixed substance to be tested is moved to the detection module 4 for detection through the third conveying mechanism 83. It should be noted that in the above embodiment, the incubation turntable 21 is described as having a motion state of rotating in the clockwise direction, but the present application is not limited thereto. In some embodiments, the incubation turntable 21 can also rotate in the counterclockwise direction.

[0068] Therefore, combined with the arrangement relationship of the magnetic separation module 3, the detection module 4, the placement module 5, the reagent module 6, the consumables module 7, the first conveying mechanism 81, the second conveying mechanism 82, the third conveying mechanism 83 and the incubation module 2, during the detection process of the electrochemiluminescence immunoassay detector 100, the movement trajectory of the second test tube in the entire detection process is single, which is beneficial to shorten the total detection time of the electrochemiluminescence immunoassay detector 100 and improve the detection efficiency of the electrochemiluminescence immunoassay detector 100.

[0069] See also Figure 2 As shown, since the human sample is transported from the sample placement module 5 to the second test tube placed on the incubation turntable 21 through the first swing arm 810, the reagent is transported from the reagent module 6 to the second test tube placed on the incubation turntable 21 through the second swing arm 820. In one embodiment, the matching position of the first swing arm 810 and the incubation turntable 21 (i.e., the relative position of the first swing arm 810 and the incubation turntable 21 when the first swing arm 810 injects the human sample into the second test tube) is different from the matching position of the second swing arm 820 and the incubation turntable 21 (i.e., the relative position of the second swing arm 820 and the incubation turntable 21 when the second swing arm 820 injects the reagent into the second test tube), so that the second swing arm 820 can swing at the same time during the swinging of the first swing arm 810, which is beneficial to shorten the total detection time of the electrochemiluminescence immunoassay instrument 100 and improve the detection efficiency of the electrochemiluminescence immunoassay instrument 100. Figure 1 As shown, when both the first swing arm 810 and the second swing arm 820 are swinging, the movement of the partial structure of the magnetic separation module 3 (i.e., the structure for taking the second test tube containing the mixed substance and placing it on the incubation turntable 21 in the magnetic separation module 3) and the partial structure of the third conveying mechanism 83 (i.e., the structure for extracting the mixed substance to be tested from the second test tube) will not be affected. This is also beneficial to shortening the total detection time of the electrochemiluminescence immunoassay instrument 100 and improving the detection efficiency of the electrochemiluminescence immunoassay instrument 100.

[0070] See also Figure 1 As shown, according to the electrochemiluminescence immunoassay instrument 100 of the present application, since the magnetic separation module 3 and the detection module 4 are arranged on the same side of the incubation module 2 in the first direction, and the layout module 5 is arranged on the other side of the incubation module 2, and the reagent module 6 and the consumable module 7 are respectively located on both sides of the incubation module 2 in the second direction, and the first conveying mechanism 81 is arranged on one side of the incubation module 2 away from the consumable module 7 in the second direction, the second conveying mechanism 82 is arranged between the incubation module 2 and the reagent module 6, and the third conveying mechanism 83 is arranged between the magnetic separation module 3 and the detection module 4, so that the magnetic separation module 3, the third conveying mechanism 83, the detection module 4, the consumable module 7, the layout module 5, the first conveying mechanism 81, the reagent module 6, and the second conveying mechanism 82 are arranged along the circumference of the incubation module 2.

[0071] In this way, the positions of the various components are arranged reasonably while meeting the requirements of the detection steps of the electrochemiluminescence immunoassay instrument 100, so that the internal space layout of the electrochemiluminescence immunoassay instrument 100 is compact, and the electrochemiluminescence immunoassay instrument 100 is highly integrated and miniaturized. That is, while meeting the detection requirements, the electrochemiluminescence immunoassay instrument 100 has the advantage of a small size, so that the electrochemiluminescence immunoassay instrument 100 can be placed on a countertop for use, and it is also convenient for the operator to carry the electrochemiluminescence immunoassay instrument 100 by hand.

[0072] Based on the arrangement relationship of the above-mentioned components relative to the incubation module 2, and the coordination relationship between the first swing arm 810, the second swing arm 820, the magnetic separation module 3 and the third conveying mechanism 83 and the incubation turntable 21 respectively, the total detection time of the electrochemiluminescence immunoassay instrument 100 can also be shortened and the detection efficiency of the electrochemiluminescence immunoassay instrument 100 can be improved.

[0073] In summary, the various components of the electrochemiluminescence immunoassay instrument 100 of the present application are reasonably and compactly laid out, achieving the effect of high integration and miniaturization of the electrochemiluminescence immunoassay instrument 100, and the total detection time of the electrochemiluminescence immunoassay instrument 100 is short, with the advantage of high detection efficiency.

[0074] See also Figure 1 , Figure 2 , Figure 4 and Figure 7 As shown, in some embodiments of the present application, the incubation module 2 may further include at least one of a first needle cleaning member 22, a second needle cleaning member 23, a third needle cleaning member 24, and a first mixing member 25. The first needle cleaning member 22 and the third needle cleaning member 24 are both arranged in the arc trajectory of the first needle 811 assembled on the first swing arm 810, the second needle cleaning member 23 is arranged in the arc trajectory of the second needle 821 assembled on the second swing arm 820, and the first mixing member 25 is arranged between the incubation turntable 21 and the consumable module 7 in the second direction.

[0075] For example, see Figure 1 , Figure 2 , Figure 4 and Figure 7As shown, in one embodiment of the present application, the incubation module 2 includes a first needle cleaning member 22, a second needle cleaning member 23, a third needle cleaning member 24, and a first mixing member 25. The first needle cleaning member 22 and the third needle cleaning member 24 are both configured to clean the first needle 811 of the first swing arm 810, and the second needle cleaning member 23 is configured to clean the second needle 821 of the second swing arm 820. The first mixing member 25 is configured to shake the second test tube containing the mixed substance to fully mix the human sample, the first reagent, the second reagent, and the magnetic bead reagent in the mixed substance.

[0076] The first needle 811 and the second needle 821 are made of stainless steel, and a first needle cleaning component 22 and a third needle cleaning component 24 for cleaning the first needle 811, and a second needle cleaning component 23 for cleaning the second needle 821 are provided in the incubation module 2, so that the first needle 811 and the second needle 821 can be reused, thereby eliminating the need to set up a module for replacing the needle. Since the module for replacing the needle needs to meet the needs of disassembly and installation of the needle, and also needs to set up a mechanism for transporting old needles and replacing needles, the module for replacing the needle is larger in size than the first needle cleaning component 22 or the second needle cleaning component 23 or the third needle cleaning component 24. Therefore, according to the electrochemiluminescence immunoassay instrument 100 of the present application, a first needle cleaning component 22 and / or a second needle cleaning component 23 and / or a third needle cleaning component 24 is provided, which is beneficial to the reasonable and compact layout of the various components in the electrochemiluminescence immunoassay instrument 100, so as to further realize the highly integrated and miniaturized effect of the electrochemiluminescence immunoassay instrument 100.

[0077] See also Figure 2 The first arc trajectory S1 shown in the figure is the movement trajectory of the first needle 811 in the process of the first swing arm driver 812 driving the first swing arm 810 to move. The first needle cleaning member 22 and the third needle cleaning member 24 both coincide with the first arc trajectory S1. Along the extension direction of the first arc trajectory S1, the first needle cleaning member 22 and the third needle cleaning member 24 are both located between the sample placement module 5 and the incubation turntable 21, so that when the first needle 811 moves from the incubation turntable 21 to the sample placement module 5 after completing each conveying procedure, the first needle cleaning member 22 and / or the third needle cleaning member 24 cleans the first needle 811, which is beneficial to shorten the total detection time of the electrochemiluminescence immunoassay instrument 100 and achieve the effect of high detection efficiency.

[0078] For example, combined with Figure 1 , Figure 2 , Figure 4 and Figure 7As shown, after the first needle 811 completes one conveying, during the process of the first needle 811 moving from the incubation turntable 21 toward the sample placement module 5 along the first arc trajectory line S1, the first swing arm driver 812 drives the first swing arm 810 to swing, so that the first needle 811 corresponds to the first needle cleaning part 22, and then the first swing arm driver 812 drives the first swing arm 810 to descend, so that the first needle 811 moves into the first needle cleaning part 22 for one cleaning. After the first needle 811 completes one cleaning, the first swing arm driver 812 drives the first swing arm 810 to rise, and then the first swing arm driver 812 drives the first swing arm 810 to swing, so that the first needle 811 corresponds to the third needle cleaning part 24, and then the first swing arm driver 812 drives the first swing arm 810 to descend, so that the first needle 811 moves into the third needle cleaning part 24 for a second cleaning. After the first needle 811 completes the second cleaning, the first swing arm driver 812 drives the first swing arm 810 to rise, and then the first swing arm driver 812 drives the first swing arm 810 to swing, so that the first needle 811 corresponds to the first needle cleaning part 22 again, and then the first swing arm driver 812 drives the first swing arm 810 to descend, so that the first needle 811 moves into the first needle cleaning part 22 for three cleanings. After the first needle 811 completes the three cleanings, the first swing arm driver 812 drives the first swing arm 810 to rise, and then the first swing arm driver 812 drives the first swing arm 810 to swing toward the sample placement module 5, so that the first needle 811 can perform secondary sampling.

[0079] Therefore, according to the electrochemiluminescence immunoassay detector 100 of the present application, a first needle cleaning component 22 and / or a third needle cleaning component 24 are provided in the incubation module 2 to achieve the effect of cleaning the first needle 811, so that the first needle 811 does not affect each other in two adjacent sampling processes, thereby ensuring the accuracy of the detection. At the same time, there is no need to set up a module for replacing the first needle 811, which is beneficial to the reasonable and compact layout of various components in the electrochemiluminescence immunoassay detector 100, so as to further achieve the effect of high integration and miniaturization of the electrochemiluminescence immunoassay detector 100.

[0080] See also Figure 2 The second arc trajectory S2 shown in the figure is the movement trajectory of the second needle 821 in the process of the second swing arm driver 822 driving the second swing arm 820 to move. The second needle cleaning member 23 coincides with the second arc trajectory S2. Along the extension direction of the second arc trajectory S2, the second needle cleaning member 23 is located between the reagent module 6 and the incubation turntable 21, so that the second needle 821 is cleaned by the second needle cleaning member 23 in the process of moving from the incubation turntable 21 to the sample placement module 5 after completing each conveying procedure, thereby shortening the total detection time of the electrochemiluminescence immunoassay instrument 100 and achieving the effect of high detection efficiency.

[0081] For example, see Figure 1 , Figure 2 , Figure 4 and Figure 7 As shown, after the second needle 821 completes the delivery of the first reagent, during the process of the second needle 821 moving from the incubation turntable 21 toward the reagent module 6 along the second arc trajectory line S2, the second swing arm driver 822 drives the second swing arm 820 to swing, so that the second needle 821 corresponds to the second needle cleaning part 23, and then the second swing arm driver 822 drives the second swing arm 820 to descend, so that the second needle 821 moves into the second needle cleaning part 23 for cleaning. After the second needle 821 completes the cleaning, the second swing arm driver 822 drives the second swing arm 820 to rise, and then the second swing arm driver 822 drives the second swing arm 820 to swing, so that the second needle 821 extracts the second reagent, and then the second swing arm driver 822 drives the second swing arm 820 to swing from the reagent module 6 to the incubation turntable 21, so as to inject the second reagent into the second test tube. After completing the delivery of the second reagent, the second swing arm driver 822 drives the second swing arm 820 to swing so that the second needle 821 corresponds to the second needle cleaning part 23, so that the second needle cleaning part 23 cleans the second needle 821. After the second needle 821 is cleaned, the second needle 821 is used to extract the magnetic bead reagent.

[0082] Therefore, according to the electrochemiluminescence immunoassay detector 100 of the present application, a second needle cleaning part 23 is provided in the incubation module 2 to achieve the effect of cleaning the second needle 821, so that the second needle 821 does not affect each other during two adjacent reagent extraction processes, thereby ensuring the accuracy of the detection. At the same time, there is no need to set up a module for replacing the second needle 821, which is conducive to the reasonable and compact layout of various components in the electrochemiluminescence immunoassay detector 100, so as to further achieve the effect of high integration and miniaturization of the electrochemiluminescence immunoassay detector 100.

[0083] It should be noted that, in some embodiments, the first needle cleaning member 22 has a spring portion and a blowing portion. Specifically, the spring portion is used to drive the cleaning liquid to make the cleaning liquid flow in a spring state, so that the cleaning liquid can flow into the needle tube channel of the first needle 811, and achieve the function of fully cleaning the inner wall and the outside of the first needle 811, ensuring the cleanliness of the first needle 811, and after the cleaning is completed with the cleaning liquid, the blowing portion blows air to the first needle 811 to dry it, ensuring the dryness of the first needle 811, and avoiding the phenomenon of liquid hanging on the first needle 811.

[0084] The second needle cleaning member 23 has a negative pressure portion, which forms a negative pressure in the second needle cleaning member 23. After the second needle cleaning member 23 uses the cleaning liquid to clean the second needle 821, under the action of the negative pressure portion, the second needle 821 is prevented from being covered with liquid, thereby ensuring the dryness of the second needle 821.

[0085] See also Figure 2 and Figure 8 As shown, in some embodiments of the present application, the magnetic separation module 3 includes a first clamping mechanism 31, a liquid extraction / injection mechanism 32, a magnetic separation component 33, a cleaning / liquid storage component 34, and a second mixing component 35. The magnetic separation module 3 has a horizontal track line S3 parallel to the second direction, which can also be understood as that after the magnetic separation module 3 and the incubation module 2 are respectively assembled in the housing 1, the horizontal track line S3 of the magnetic separation module 3 is parallel to the second direction of the incubation module 2.

[0086] In the magnetic separation module 3, the first clamping mechanism 31 and the extraction / injection mechanism 32 can both translate along the horizontal trajectory line S3, and the incubation turntable 21, the magnetic separation component 33, the cleaning / liquid storage component 34 and the second mixing component 35 are all located within the horizontal trajectory line S3. The first clamping mechanism 31 is configured to take and place consumables on the incubation turntable 21, the magnetic separation component 33 and the second mixing component 35. The magnetic separation component 33 is provided with a cylindrical magnetic component, and a placement space is formed in the magnetic component, and the placement space is used to place consumables. The extraction needle 321 of the extraction / injection mechanism 32 is configured to extract liquid from the consumable, and the injection needle 322 of the extraction / injection mechanism 32 is configured to add liquid into the consumable. The cleaning / liquid storage part 34 includes a fourth needle cleaning part 341 and a liquid storage part 342 . The fourth needle cleaning part 341 is configured to clean the liquid extraction needle 321 , and the liquid storage part 342 is used to store the liquid to be extracted by the liquid extraction needle 321 .

[0087] Exemplarily, in one embodiment, in combination Figure 2 and Figure 8As shown, along the extension direction of the horizontal trajectory line S3, the first clamping mechanism 31 moves toward the incubation turntable 21, so that the first clamping mechanism 31 takes out the second test tube containing the mixed substance from the incubation turntable 21, and places the second test tube in the placement space of the magnetic separation part 33. Since the substance to be tested of the mixed substance has magnetic beads, the magnetic beads and the magnetic part are magnetically matched, so that the substance to be tested and the magnetic part are magnetically adsorbed. It should be noted that the magnetic adsorption referred to here refers to the substance to be tested and the magnetic part under the action of the magnetic force, and the substance to be tested in the second test tube moves toward the magnetic part. Since the second test tube is placed in the placement space formed by the annular magnetic part, it can be understood that the substance to be tested is attached to the inner peripheral wall of the second test tube under the action of the magnetic force. It is also worth noting that since the magnetic part is annular, the inner peripheral wall of the magnetic part (i.e., the peripheral wall of the placement space) can be used to generate magnetic force with the substance to be tested containing magnetic beads. Therefore, more of the substance to be tested adheres to the inner wall of the second test tube under the action of magnetic force, avoiding being drawn away by the liquid drawing needle 321 of the liquid drawing / injection mechanism 32, thereby ensuring the content of the substance to be tested in the second test tube and the subsequent detection accuracy.

[0088] After the second test tube is placed on the magnetic separator 33, the extraction / injection mechanism 32 moves along the horizontal trajectory line S3 toward the magnetic separator 33, so that the extraction needle 321 of the extraction / injection mechanism 32 extends into the second test tube to extract at least part of the mixed liquid in the mixed substance, and then the injection needle 322 of the extraction / injection mechanism 32 adds new reagent into the second test tube, so that the new reagent is mixed with the substance to be tested to form a mixed substance to be tested. It should be supplemented that, after the extraction needle 321 extracts the mixed liquid in the mixed substance, the extraction needle 321 moves into the fourth needle cleaning part 341 in the cleaning / liquid storage part 34, so that the fourth needle cleaning part 341 cleans the extraction needle 321 to ensure the cleanliness of the extraction needle 321 and avoid affecting the next extraction. In addition, before the injection needle 322 adds new reagent into the second test tube, the injection needle 322 extracts new reagent from the liquid storage part 342 so that the injection needle 322 can add new reagent into the second test tube.

[0089] The first clamping mechanism 31 moves the second test tube containing the mixed substance to be tested to the second mixing member 35, and the mixed substance to be tested is fully shaken under the action of the second mixing member 35. After the second mixing member 35 has fully shaken the mixed substance to be tested, the first clamping mechanism 31 moves the second test tube containing the mixed substance to be tested to the incubation turntable 21, so that the mixed substance to be tested can be incubated in the incubation turntable 21, and the third conveying mechanism 83 can extract the mixed substance to be tested to the detection module 4 for detection.

[0090] See also Figure 1 and Figure 2As shown, in some embodiments of the present application, the sample placement module 5 is provided with a sample injection channel 50a, a collection channel 50b and a sample outlet channel 50c, the collection channel 50b is connected to the sample injection channel 50a and the sample outlet channel 50c, and the extension direction of the collection channel 50b is perpendicular to the extension direction of the sample injection channel 50a and the extension direction of the sample outlet channel 50c. The sample placement module 5 also includes a first drive component, which is configured to drive the sample loading part to move along the sample injection channel 50a, the collection channel 50b and the sample outlet channel 50c in sequence.

[0091] For example, see Figure 2 As shown, in one embodiment of the present application, the extension direction of the injection channel 50a is parallel to the extension direction of the sample outlet channel 50c, and the extension direction of the collection channel 50b is perpendicular to the extension direction of the injection channel 50a and the extension direction of the sample outlet channel 50c. Specifically, the ports at both ends of the injection channel 50a are respectively the first port and the second port, and the ports at both ends of the sample outlet channel 50c are respectively the third port and the fourth port, wherein the second port and the third port are both connected to the collection channel 50b.

[0092] The test tube holder equipped with at least one first test tube is placed in the sample inlet channel 50a. Under the action of the first driving component, the test tube holder moves along the sample inlet channel 50a toward the collection channel 50b and enters the collection channel 50b. Then the first driving component continues to drive the test tube holder to move along the collection channel 50b, so that the test tube holder moves toward the sample outlet channel 50c. In the process of the test tube holder moving along the collection channel 50b from the side of the sample inlet channel 50a toward the sample outlet channel 50c, when a first test tube placed in the test tube holder corresponds to the first arc trajectory line S1, the first needle 811 of the first swing arm 810 can extract the human body sample in the first test tube. Figure 2 As shown, in this embodiment, the test tube holder can be loaded with five first test tubes. When the first driving component drives the test tube holder to move from the sample inlet channel 50a to the sample outlet channel 50c, the first test tube close to the sample outlet channel 50c first corresponds to the first arc trajectory line S1. After the first needle 811 finishes extracting the human body sample in the first test tube, the first driving component further drives the test tube holder to move, so that the subsequent first test tubes correspond to the first arc trajectory line S1 in sequence.

[0093] It should be noted that, according to the sample placement module 5 of the present application, since there are a sample injection channel 50a, a collection channel 50b and a sample outlet channel 50c, and the collection channel 50b is connected to the sample injection channel 50a and the sample outlet channel 50c, and the extension direction of the collection channel 50b is perpendicular to the extension direction of the sample injection channel 50a and the extension direction of the sample outlet channel 50c, each test tube holder can move in one direction along the sample injection channel 50a, the collection channel 50b and the sample outlet channel 50c in sequence, so as to avoid the test tube holder from exiting along the previously moved channel, so that when there are at least two groups of test tubes that need to be detected (the test tube group is the test tube in each test tube holder), the two groups of test tubes can be placed in the sample placement module 5 at the same time, that is, when the previous test tube group is located in the collection channel 50b or the sample outlet channel 50c, the latter test tube group can be placed in the sample injection channel 50a. This is also conducive to shortening the total detection time of the electrochemiluminescence immunoassay instrument 100 and achieving the effect of high detection efficiency.

[0094] See also Figure 2 As shown, in some embodiments of the present application, the first driving assembly includes a first pusher 53a and a second pusher 53b, wherein the push claw of the first pusher 53a is configured to push the sample loading member in the injection channel 50a to the collection channel 50b, and the push claw of the second pusher 53b is configured to push the sample loading member in the collection channel 50b to the sample outlet channel 50c. The first driving assembly includes a third pusher 53c, and the third pusher 53c is configured to drive the sample loading member to move from the injection channel 50a to the sample outlet channel 50c along the extension direction in the collection channel 50b. It should be noted that, in one embodiment, the sample loading member is a test tube holder for placing the first test tube.

[0095] For example, see Figure 2 As shown, in one embodiment of the present application, the first pusher 53a and the second pusher 53b are both push claws, so that the first pusher 53a can push the test tube holder from the sample inlet channel 50a to the collection channel 50b, and the second pusher 53b can push the test tube holder from the collection channel 50b to the sample outlet channel 50c. In another embodiment of the present application, the first pusher 53a and / or the second pusher 53b are push plates.

[0096] After the first pusher 53a pushes the test tube holder into the collection channel 50b, the third pusher 53c contacts the test tube holder, and the test tube holder is moved along the extension direction of the collection channel 50b by the third pusher 53c. It should be supplemented that in the height direction of the sample placement module 5, the third pusher 53c is staggered with the first pusher 53a and the second pusher 53b, and the height of the liquid flowing into the third pusher 53c is higher than that of the first pusher 53a and the second pusher 53b, thereby avoiding interference between the third pusher 53c and the first pusher 53a and the second pusher 53b, and ensuring that the third pusher 53c can drive the test tube holder to move along the collection channel 50b.

[0097] See also Figure 2 As shown, in some embodiments of the present application, the sample placement module 5 may further include a sample placement module housing 51, and the sample placement module housing 51 is provided with a partition boss 52, and the partition boss 52 defines a sample inlet channel 50a, a collection channel 50b and a sample outlet channel 50c in the sample placement module housing 51. The partition boss 52 is provided with a spare sample loading space 520, and the spare sample loading space 520 is arranged in the arc trajectory of the first needle 811 mounted on the first swing arm 810. For example, see Figure 2 As shown, in one embodiment of the present application, the spare sample loading space 520 is used to place the first test tube, so that the first needle 811 can extract the human sample inside the first test tube placed in the spare sample loading space 520. Since the spare sample loading space 520 is provided for placing the first test tube, when the number of first test tubes to be used is greater than the number of first test tubes that can be placed in the test tube holder, the excess first test tubes can be directly placed in the spare sample loading space 520. Since there is no need to take the test tube holder for placing the first test tube again, this is also conducive to shortening the total detection time of the electrochemiluminescence immunoassay instrument 100, and achieving the effect of high detection efficiency.

[0098] See also Figure 2 , Figures 9 to 11 As shown, in some embodiments of the present application, the reagent module 6 includes a third drive component 61 and a reagent turntable 62, the reagent turntable 62 is assembled in the assembly space 6110 of the third drive component 61, the reagent turntable 62 is assembled in the assembly space 6110, and the third drive component 61 drives the reagent turntable 62 to rotate in the form of self-rotation, and the reagent turntable 62 is used to place at least one reagent group.

[0099] For example, see Figure 2As shown, due to the use of a reagent turntable 62 for placing a reagent group, each reagent group is provided with a test tube containing a first reagent, a test tube containing a second reagent, and a test tube containing a magnetic bead reagent. When the reagent group is assembled on the reagent turntable 62, the three test tubes in the reagent group are arranged along the radial arrangement of the reagent turntable 62, and are swung in conjunction with the second swing arm 820 so that the second needle 821 of the second swing arm 820 can extract the reagents in the three test tubes respectively. Thus, according to the reagent module 6 of the present application, the reagent turntable 62 is used to place the reagent group, so that the reagent module 6 can still be placed with a large amount of reagent groups under the requirement of small size, which can not only realize the miniaturization design of the electrochemiluminescence immunoassay instrument 100, but also reduce the frequency of the operator replenishing the reagent group, and improve the user experience.

[0100] See also Figure 2 , Figures 9 to 11 As shown, in some embodiments of the present application, the third drive assembly 61 includes an assembly housing 611 and a first driver 612, and the first driver 612 is assembled in the assembly housing 611, and the first driver 612 drives the reagent turntable 62 to rotate. An assembly space 6110 is formed in the assembly housing 611, and the assembly space 6110 is also provided with a drive rack 611c, and the drive rack 611c is arranged along the circumferential extension of the assembly space 6110, and the drive rack 611c is meshed with the meshing gear 621 arranged on the reagent turntable 62, and the meshing gear 621 rotates to drive a reagent tube in the reagent group to rotate. The reagent module 6 also includes a temperature regulating member 63, and the temperature regulating member 63 is fitted with the assembly housing 611, and the temperature value in the assembly space 6110 is adjusted by the temperature regulating member 63. For example, in one embodiment, by under the effect of the temperature regulating member 63, the temperature value in the assembly space 6110 is 17 degrees Celsius. It should be supplemented that the temperature regulating member 63 can release heat and / or absorb the heat in the external environment to the external environment. It can be understood that the temperature regulating component 63 of one embodiment only has the ability to release heat to the external environment, or, the temperature regulating component 63 of another embodiment only has the ability to absorb heat from the external environment, or, the temperature regulating component 63 of yet another embodiment has both the ability to release heat to the external environment and the ability to absorb heat from the external environment.

[0101] For example, see Fig. 9 As shown, in one embodiment of the present application, the assembly housing 611 includes a housing wall 611a and a bottom plate 611b. The housing wall 611a is a cylindrical body, and the bottom plate 611b is a circular plate. The housing wall 611a and the bottom plate 611b cooperate to define an assembly space 6110, and a drive rack 611c disposed in the assembly space 6110 is extended along the axial direction of the housing wall 611a, that is, the drive rack 611c is extended along the circumferential direction of the assembly space 6110. Fig.10 As shown, when the reagent turntable 62 is assembled in the assembly space 6110, each meshing gear 621 arranged in the reagent turntable 62 is meshedly connected with the driving rack 611c, so that when the first driver 612 drives the reagent turntable 62 to rotate, the meshing gear 621 can rotate. It should be noted that a test tube containing a magnetic bead reagent is provided in the reagent set of an embodiment of the present application. When the reagent set is assembled on the reagent turntable 62, the test tube containing the magnetic bead reagent is fixedly matched with the meshing gear 621, so that when the reagent turntable 62 rotates, the test tube containing the magnetic bead reagent rotates, thereby preventing the magnetic beads in the magnetic bead reagent from settling under the action of gravity.

[0102] See also Figure 1 As shown, in one embodiment of the present application, the shape of the driving rack 611c is a circular ring. However, the present application is not limited to this. For example, the driving rack 611c can be arc-shaped, and multiple driving racks 611c can be arranged in sequence to form a ring, so that any driving rack 611c is suitable for engaging with any meshing gear 621. It is worth noting that among the multiple driving racks 611c, two adjacent driving racks 611c can be spaced apart.

[0103] Combination Fig. 9 and Fig.11 As shown, the temperature regulating member 63 is fitted with the shell wall member 611a and / or the bottom plate member 611b, so that the temperature regulating member 63 indirectly adjusts the temperature value in the assembly space 6110 through the assembly shell 611, so that the reagent can be maintained within a suitable temperature range to ensure the activity of the reagent. In addition, it is also worth noting that the temperature regulating member 63 is located outside the assembly space 6110 to avoid interference between the temperature regulating member 63 and the reagent turntable 62. For example, see Fig.11 As shown, in one embodiment of the present application, the temperature adjustment member 63 is disposed on the bottom plate member 611 b.

[0104] See also Fig. 9 and Fig.11 As shown, in one embodiment of the present application, the first driver 612 includes a driving motor 612a, a transmission belt 612b and a driving disk 612c. The driving motor 612a and the driving disk 612c are both equipped with a bottom plate 611b, and the driving disk 612c is also suitable for connecting with the reagent turntable 62, and the transmission belt 612b is transmission-connected between the driving motor 612a and the driving disk 612c, and the driving motor 612a drives the transmission belt 612b to rotate, so that the transmission belt 612b drives the driving disk 612c to rotate, thereby achieving the effect of the reagent turntable 62 rotating.

[0105] See also Figure 2 , Figure 5 and Fig.12As shown, in some embodiments of the present application, the consumable module 7 includes a consumable module bottom plate 71, a stage 72 and a third clamping mechanism 73. The consumable module bottom plate 71 has a vertical third direction and a fourth direction. When the consumable module 7 and the incubation module 2 are assembled in the housing 1, the third direction of the consumable module bottom plate 71 is parallel to the first direction of the incubation module 2, and the fourth direction of the consumable module bottom plate 71 is parallel to the second direction of the incubation module 2. And the stage 72 is slidably assembled on the consumable module bottom plate 71 along the fourth direction, and the stage 72 is used to place consumables. It should be noted that in some embodiments of the present application, the above-mentioned consumables are second test tubes. The third clamping mechanism 73 includes a fourth drive assembly 731 and a clamp 732. The fourth drive assembly 731 drives the clamp 732 to move along the third direction and the fourth direction. The clamp 732 is configured to take and place the consumables on the stage 72 and the incubation module 2.

[0106] For example, see Figure 2 , Figure 5 and Fig.12 As shown, in one embodiment of the present application, since the loading platform 72 is slidably mounted on the consumable module bottom plate 71 along the fourth direction, the loading platform 72 can be loaded into or removed from the consumable module bottom plate 71 along the third direction. Figure 6 As shown, such an arrangement enables the stage 72 to be loaded into and removed from the consumable module base plate 71 from the side of the electrochemiluminescence immunoassay instrument 100, thereby eliminating the need to assemble and detach the stage 72 from the consumable module base plate 71 in the thickness direction of the consumable module base plate 71. In this way, part of the structure of the third clamping mechanism 73 can be located above the stage 72 to make the structure of the consumable module 7 compact, thereby realizing a miniaturized design of the electrochemiluminescence immunoassay instrument 100.

[0107] The third clamping mechanism 73 is used to place the second test tube placed on the stage 72 on the incubation turntable 21. After the second test tube is filled with the mixed substance, the third clamping mechanism 73 moves the second test tube from the incubation turntable 21 to the first mixing member 25, so that the substances in the mixed substance are fully mixed. After the mixed substance is fully mixed, the third clamping mechanism 73 transports the second test tube to the incubation turntable 21 again, so that the incubation turntable 21 incubates the mixed substance.

[0108] It should be noted that, in some embodiments of the present application, a thermostat is provided in the incubation module 2, and the thermostat is used to control the temperature of the incubation module 2 so that the temperature placed in the incubation turntable 21 is always within a suitable temperature range for incubation. For example, in one embodiment, the thermostat can control the temperature of the incubation module 2 to be maintained at thirty-seven degrees Celsius. It should be supplemented that the thermostat can release heat to the external environment and\or absorb heat from the external environment. It can be understood that the thermostat of one embodiment only has the ability to release heat to the external environment, or the thermostat of another embodiment only has the ability to absorb heat from the external environment, or the thermostat of another embodiment has both the ability to release heat to the external environment and the ability to absorb heat from the external environment.

[0109] See also Figure 2 , Figure 5 and Fig.12 As shown, in some embodiments of the present application, the fourth drive assembly 731 includes a sliding gantry 731a and a second driver 731b, the second driver 731b is mounted on the sliding gantry 731a, and the second driver 731b is configured to drive the third clamping mechanism 73 to move along the third direction. The sliding gantry 731a is slidably mounted with the consumable module bottom plate 71 along the fourth direction, so that the third clamping mechanism 73 can move along the third direction and the fourth direction, and it should also be noted that the third clamping mechanism 73 can also move in a direction perpendicular to the third direction and the fourth direction, that is, the height direction of the sliding gantry 731a, so that the third clamping mechanism 73 can take and place consumables (for example: the second test tube).

[0110] In addition, the sliding gantry 731a and the consumable module base plate 71 jointly define a through space 74 that runs through the fourth direction, and the loading platform 72 is suitable for passing through the through space 74 to avoid interference with the loading platform 72 during the movement of the sliding gantry 731a along the fourth direction, and to avoid interference between the loading platform 72 and the sliding gantry 731a during the movement of the loading platform 72 along the fourth direction to load and remove the consumable module base plate 71.

[0111] See also Figure 1 and Fig.13 As shown, in some embodiments of the present application, the third transport mechanism 83 has a third needle 831, and the third needle 831 is configured to extract substances from the incubation turntable 21 into the detection module 4, so that the detection module 4 detects the extracted substances.

[0112] For example, see Figure 1 and Fig.13As shown, in one embodiment of the present application, the third needle 831 extracts the mixed substance to be tested from the second test tube containing the mixed substance to be tested, and transports the mixed substance to be tested to the detection module 4. After the third needle 831 transports the mixed substance to be tested to the detection module 4, the third needle 831 moves into the fifth needle cleaning part 832 of the third conveying mechanism 83, and the third needle 831 is cleaned by the fifth needle cleaning part 832.

[0113] After the third needle 831 is cleaned, the third needle 831 is configured to extract the detection reagent from the detection reagent storage part 833 in the third delivery mechanism 83 and deliver the detection reagent to the detection module 4 .

[0114] In some embodiments of the present application, the electrochemiluminescent immunoassay instrument 100 according to the present application is suitable for the competition method, which is a commonly used method for detecting small molecule antigens. This method determines the concentration of the test object by measuring the competitive relationship between the labeled antigen and the antigen to be tested. The principle of the competition method is based on the specific binding of antigen and antibody. In the reaction system, the labeled antigen and the antigen to be tested exist at the same time, and they will bind to the fixed antibody. If the sample to be tested contains the antigen to be tested, it will compete with the labeled antigen for binding to the antibody, thereby reducing the chance of the labeled antigen binding to the antibody. By measuring the binding amount of the labeled antigen, the concentration of the antigen to be tested can be calculated.

[0115] The specific steps of competition law are as follows:

[0116] 1. Coated antibody: fix the specific antibody on a solid phase carrier.

[0117] 2. Add sample: Mix the sample to be tested, labeled antigen and antibody and incubate for a period of time.

[0118] 3. Washing: Remove unbound substances.

[0119] 4. Detection: Measure the binding amount of the labeled antigen and calculate the concentration of the antigen to be tested through the standard curve.

[0120] It should be noted that the advantages of the competitive method include simple operation, high sensitivity, and good repeatability, and it is particularly suitable for the detection of small molecules.

[0121] In some embodiments of the present application, the detection module 4 can use the current method to detect the abnormal state of the liquid being tested in the measuring pool. Under the action of electrochemistry, the electrodes consume different amounts of electrical energy in different states of the liquid being tested. By monitoring the voltage and current of the electrodes and comparing the electrical energy consumption, it is possible to identify whether the liquid being tested contains bubbles or other abnormal states. The abnormal state test results can be identified in a timely manner and retested.

[0122] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0123] The above-described embodiments only express several implementation methods of the present application, and the descriptions thereof are relatively specific and detailed, but they cannot be construed as limiting the scope of the patent application. It should be pointed out that, for a person of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present application, and these all belong to the protection scope of the present application. Therefore, the protection scope of the patent application shall be subject to the attached claims.

Claims

1. An electrochemiluminescence immunoassay instrument, characterized in that: include: case; and an incubation module, a magnetic separation module, a detection module, a sample placement module, a reagent module, a consumables module, a first conveying mechanism, a second conveying mechanism and a third conveying mechanism assembled in the housing; The incubation module has a first direction and a second direction that are perpendicular to each other. In the first direction, the magnetic separation module and the detection module are both arranged on one side of the incubation module, and the placement module is arranged on the other side of the incubation module. In the second direction, the reagent module and the consumables module are respectively located on both sides of the incubation module. In the second direction, the first conveying mechanism is arranged on a side of the incubation module away from the consumables module, and the first swing arm of the first conveying mechanism is configured to convey the material in the placement module to the incubation turntable of the incubation module; The second conveying mechanism is arranged between the incubation module and the reagent module, and the second swing arm of the second conveying mechanism is configured to convey at least one substance in the reagent module to the incubation turntable; The magnetic separation module is configured to perform magnetic separation processing on the substances placed on the incubation turntable; The third conveying mechanism is arranged between the magnetic separation module and the detection module, and is configured to convey the material in the incubation turntable that has undergone magnetic separation treatment to the detection module.

2. The electrochemiluminescence immunoassay instrument according to claim 1, characterized in that: The incubation module further includes a first needle cleaning member and / or a second needle cleaning member and / or a third needle cleaning member and / or a first mixing member; Among them, the first needle cleaning part and the third needle cleaning part are both arranged in the circular arc trajectory of the first needle assembled on the first swing arm, the second needle cleaning part is arranged in the circular arc trajectory of the second needle assembled on the second swing arm, and the first mixing part is arranged between the incubation turntable and the consumable module in the second direction.

3. The electrochemiluminescence immunoassay instrument according to claim 1, characterized in that: The magnetic separation module includes a first clamping mechanism, a liquid extraction / injection mechanism, a magnetic separation component, a cleaning / liquid storage component, and a second mixing component; The magnetic separation module has a horizontal track line parallel to the second direction, the first clamping mechanism and the liquid extraction / injection mechanism are translated along the horizontal track line, and the incubation turntable, the magnetic separation element, the cleaning / liquid storage element and the second mixing element are all located within the horizontal track line; The first clamping mechanism is configured to place the consumables on the incubation turntable, the magnetic separation element, and the second mixing element; The magnetic separator is provided with a cylindrical magnetic member, a placement space is formed in the magnetic member, and the placement space is used to place the consumables; The liquid extraction needle of the liquid extraction / injection mechanism is configured to extract liquid from the consumable material, and the liquid injection needle of the liquid extraction / injection mechanism is configured to inject liquid into the consumable material; The cleaning / liquid storage part includes a fourth needle cleaning part and a liquid storage part. The fourth needle cleaning part is configured to clean the liquid extraction needle, and the liquid storage part is used to store the liquid to be extracted by the liquid extraction needle.

4. The electrochemiluminescence immunoassay instrument according to claim 1, characterized in that: The sample placement module is provided with a sample injection channel, a collection channel and a sample output channel, the collection channel is connected to the sample injection channel and the sample output channel, and the extension direction of the collection channel is perpendicular to the extension direction of the sample injection channel and the extension direction of the sample output channel; The sample placement module further includes a first driving component, which is configured to drive the sample loading component to move along the sample injection channel, the collection channel and the sample output channel in sequence.

5. The electrochemiluminescence immunoassay instrument according to claim 4, characterized in that: The sample placement module further comprises a sample placement module housing, wherein the sample placement module housing is provided with a separation boss, and the separation boss defines the sample inlet channel, the collection channel and the sample outlet channel in the sample placement module housing; The separation boss is provided with a spare sample loading space, and the spare sample loading space is arranged in the arc track line of the first needle assembled on the first swing arm.

6. The electrochemiluminescence immunoassay instrument according to claim 4, characterized in that: The first driving assembly includes a first pushing member and a second pushing member, wherein the pushing claw of the first pushing member is configured to push the sample loading member in the sample injection channel to the collection channel, and the pushing claw of the second pushing member is configured to push the sample loading member in the collection channel to the sample output channel; The first driving assembly includes a third pushing member, and the third pushing member is configured to drive the sample loading member to move from the sample injection channel to the sample output channel along an extension direction in the collection channel.

7. The electrochemiluminescence immunoassay instrument according to claim 1, characterized in that: The reagent module includes a third drive assembly and a reagent turntable. The reagent turntable is assembled in the assembly space of the third drive assembly. The reagent turntable is assembled in the assembly space, and the third drive assembly drives the reagent turntable to rotate. The reagent turntable is used to place at least one reagent group.

8. The electrochemiluminescence immunoassay instrument according to claim 7, characterized in that: The third driving assembly includes an assembly housing and a first driver, wherein the first driver is assembled in the assembly housing, and the first driver drives the reagent turntable to rotate; The assembly space is formed in the assembly housing, and the assembly space is also provided with a driving rack, the driving rack is extended along the circumference of the assembly space, the driving rack is meshed with a meshing gear arranged on the reagent turntable, and the meshing gear rotates to drive a reagent tube in the reagent group to rotate; The reagent module further comprises a temperature regulating member, which is fitted and assembled with the assembly shell, and the temperature value in the assembly space is adjusted by the temperature regulating member.

9. The electrochemiluminescence immunoassay instrument according to claim 1, characterized in that: The consumables module comprises a consumables module bottom plate, a stage and a third clamping mechanism; In which, the consumable module base plate has a vertical third direction and a fourth direction, the third direction is parallel to the first direction, and the fourth direction is parallel to the second direction; the worktable is slidably assembled on the consumable module base plate along the fourth direction, and the worktable is used to place consumables; the third clamping mechanism includes a fourth drive component and a clamp, the fourth drive component drives the clamp to move along the third direction and the fourth direction, and the clamp is configured to take and place the consumables on the worktable and the incubation module.

10. The electrochemiluminescence immunoassay instrument according to claim 9, characterized in that: The fourth driving assembly includes a sliding gantry and a second driver, the second driver is mounted on the sliding gantry, and the second driver is configured to drive the third clamping mechanism to move along the third direction; The sliding gantry is slidably assembled with the consumable module bottom plate along the fourth direction, and the sliding gantry and the consumable module bottom plate together define a through space that passes through along the fourth direction, and the loading platform is suitable for passing through the through space.

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