Method for extracting water-soluble dietary fibers from sea buckthorn seed peel residues and application of water-soluble dietary fibers
Through the composite enzyme method and anhydrous ethanol precipitation technology, the water-soluble dietary fiber in the sea buckthorn seed skin residue is efficiently extracted, which solves the problem of resource utilization of sea buckthorn seed skin residue, and realizes efficient dietary fiber extraction, which has excellent functionality and is applicable In a variety of health and food applications.
Patent Information
- Application Number
- CN202411406377.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-10
- Publication Date
- 2025-01-03
AI Technical Summary
The rich water-soluble dietary fiber in the sea buckthorn seed skin residue has not been effectively utilized, and there is a lack of efficient extraction methods.
Complex enzyme method was adopted, which included the reaction of alkaline protease, α-amylase and saccharase under different pH conditions, and the extraction of water-soluble dietary fiber through anhydrous ethanol precipitation and freeze-drying processes.
It achieves efficient, green and safe water-soluble dietary fiber extraction, with excellent oil-holding and combined with water power, and is suitable for fat metabolism regulation, serum cholesterol reduction, food quality improvement and intestinal disease prevention.
Smart Images
Figure CN119235023A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of dietary fiber extraction, in particular to a method for extracting water-soluble dietary fiber from seabuckthorn seed skin residue and application thereof. Background Art
[0002] Seabuckthorn is a plant widely distributed in Eurasia. Its fruit is rich in vitamins, minerals, amino acids and bioactive substances, and has the functions of anti-oxidation, tumor inhibition, hypoglycemia, hypolipidemic and immune regulation. However, with the development of seabuckthorn fruit processing industry, a large number of by-products such as seabuckthorn seed skin residue have not been effectively utilized and are regarded as waste.
[0003] In recent years, with the promotion of the concept of sustainable development, how to transform these agricultural wastes into reusable resources has become a research hotspot. Dietary fiber, composed of plant cell walls, polysaccharides and lignin, has good biocompatibility and biodegradability and is widely used in food, medicine, materials and other fields.
[0004] Seabuckthorn seed peel residue is rich in dietary fiber, especially water-soluble dietary fiber. In order to utilize it as a resource, an excellent method for extracting soluble dietary fiber from seabuckthorn seed peel residue is urgently needed. Summary of the invention
[0005] In view of the deficiencies in the prior art, the present invention provides a method for extracting water-soluble dietary fiber from seabuckthorn seed peel residue and its application, which solves the problems in the extraction and application of soluble dietary fiber from seabuckthorn seed peel residue.
[0006] To achieve the above object, the present invention is implemented by the following technical scheme: A method for extracting water-soluble dietary fiber from seabuckthorn seed husk residue, comprising the following steps:
[0007] I. Put 175-200g seabuckthorn seed skin residue into a beaker, add 1700-1945ml distilled water and adjust the pH to 9.0, add 5.0g alkaline protease, and react at 50℃ for 30min;
[0008] II. Following step I, adjust the pH to 6.5, add 14.2 g of α-amylase, and react at 60°C for 30 min;
[0009] II I. Following step II, adjust the pH to 4.0, add 6.0 g of saccharifying enzyme, and react at 60°C for 30 min;
[0010] IV. Following step III, inactivate the enzyme in a boiling water bath for 5 min, cool to room temperature, centrifuge, take the supernatant, wash the precipitate with distilled water and retain the washing solution;
[0011] V. Following step IV, the washing solution was stored at 4°C, the supernatant and the washing solution were concentrated, the volume was measured, four times the volume of anhydrous ethanol was added for alcohol precipitation for 4 hours, and then centrifuged at 4000 rpm for 15 minutes, the precipitate was washed twice with anhydrous ethanol, the precipitate was freeze-dried, crushed through a 100-mesh sieve, and water-soluble dietary fiber was obtained, and then stored at 4°C;
[0012] According to the above scheme, a method for extracting water-soluble dietary fiber from seabuckthorn seed skin residue comprises the following steps:
[0013] (1) Put 175 g of seabuckthorn seed raw material into a beaker, add 1700 ml of distilled water and adjust the pH to 9.0, add 5.0 g of alkaline protease, and react at 50°C for 30 min;
[0014] (2) Adjust the pH to 6.5, add 14.2 g of α-amylase, and react at 60 °C for 30 min;
[0015] (3) Adjust the pH to 4.0, add 6.0 g of saccharifying enzyme, and react at 60°C for 30 min;
[0016] (4) inactivate the enzyme in a boiling water bath for 5 min, cool to room temperature, centrifuge at 4000 rpm for 20 min, take the supernatant after centrifugation, wash the precipitate with distilled water and retain the washing solution;
[0017] (5) Store at 4°C, concentrate the supernatant and washing solution, measure the volume, add anhydrous ethanol for alcohol precipitation, centrifuge for 15 min, wash the precipitate twice with anhydrous ethanol, freeze-dry the precipitate, pulverize it through a 100-mesh sieve to obtain water-soluble dietary fiber, and store it at 4°C.
[0018] Preferably, in step I, the seabuckthorn seed skin residue raw material is defatted seabuckthorn seed residue, and the mass ratio of seabuckthorn seed residue to distilled water is 1:9.71.
[0019] Preferably, in step I, the mass ratio of alkaline protease to seabuckthorn seed skin residue raw material is 1:35, the reaction pH is 9.0, the reaction time is 30 minutes, and the reaction temperature is 50°C.
[0020] Preferably, in step II, the mass ratio of α-amylase to seabuckthorn seed skin residue raw material is 1:12.32, the reaction pH is 4.0, the reaction time is 30 minutes, and the reaction temperature is 60°C.
[0021] Preferably, in step III, the mass ratio of saccharifying enzyme to seabuckthorn seed skin residue is 1:29.17, the pH value is 6.5, the static reaction time is 30 minutes, and the reaction temperature is 60°C.
[0022] Preferably, in step IV, the centrifugal speed of the centrifugal operation is 4000 rpm and the centrifugal time is 20 min.
[0023] Preferably, the structural properties of the water-soluble dietary fiber are measured by Fourier infrared spectroscopy, X-ray diffraction, and scanning electron microscopy;
[0024] The functional test of water-soluble dietary fiber is carried out, and the test items are: water holding capacity, oil holding capacity, swelling capacity and water binding capacity.
[0025] Preferably, a use of extracting water-soluble dietary fiber from seabuckthorn seed husk residue,
[0026] It is used in the regulation of fat metabolism and the reduction of serum cholesterol;
[0027] Used in improving food quality and preventing intestinal diseases.
[0028] The present invention provides a method and application of extracting water-soluble dietary fiber from seabuckthorn seed husk residue, which has the following beneficial effects:
[0029] 1. The present invention uses a composite enzyme and anhydrous ethanol precipitation method, which has a simple process, high extraction efficiency, is green and safe, and has no harmful substance residues.
[0030] 2. The present invention extracts water-soluble dietary fiber, and oil holding capacity plays a key role in regulating fat metabolism and lowering serum cholesterol. Cellulose with high oil holding capacity can effectively slow down the absorption rate of fat. Dietary fiber with excellent water binding capacity is particularly important in improving food quality and preventing intestinal diseases. BRIEF DESCRIPTION OF THE DRAWINGS
[0031] Figure 1 This is the infrared spectrum of seabuckthorn seed peel residue SDF;
[0032] Figure 2 X-diffraction determination of SDF of seabuckthorn seed peel residue;
[0033] Figure 3 Electron microscopic image of seabuckthorn seed peel residue SDF;
[0034] Figure 4 The results of water holding capacity, oil holding capacity, swelling capacity and combined hydraulic capacity of seabuckthorn seed skin residue SDF;
[0035] Figure 5 Cholesterol and glucose adsorption capacity of seabuckthorn seed peel residue SDF. DETAILED DESCRIPTION
[0036] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0037] Embodiment 1:
[0038] like Figure 1-Figure 5 As shown, the embodiment of the present invention provides a method for extracting water-soluble dietary fiber from seabuckthorn seed husk residue, comprising the following steps:
[0039] I. Put 175g of seabuckthorn seed skin residue into a beaker, add 1700ml of distilled water and adjust the pH to 9.0, add 5.0g of alkaline protease, and react at 50°C for 30min;
[0040] II. Following step I, adjust the pH to 6.5, add 14.2 g of α-amylase, and react at 60°C for 30 min;
[0041] II I. Following step II, adjust the pH to 4.0, add 6.0 g of saccharifying enzyme, and react at 60°C for 30 min;
[0042] IV. Following step III, inactivate the enzyme in a boiling water bath for 5 min, cool to room temperature, centrifuge, take the supernatant, wash the precipitate with distilled water and retain the washing solution;
[0043] V. Following step IV, the washing solution was stored at 4°C, the supernatant and washing solution were concentrated, the volume was measured, four times the volume of anhydrous ethanol was added for alcohol precipitation for 4 hours, and then centrifuged at 4000 rpm for 15 minutes, the precipitate was washed twice with anhydrous ethanol, the precipitate was freeze-dried, crushed through a 100-mesh sieve, and water-soluble dietary fiber was obtained, which was then stored at 4°C.
[0044] The method for extracting water-soluble dietary fiber from seabuckthorn seed skin residue is as follows:
[0045] (1) Put 175 g of seabuckthorn seed raw material into a beaker, add 1700 ml of distilled water and adjust the pH to 9.0, add 5.0 g of alkaline protease, and react at 50°C for 30 min;
[0046] (2) Adjust the pH to 6.5, add 14.2 g of α-amylase, and react at 60 °C for 30 min;
[0047] (3) Adjust the pH to 4.0, add 6.0 g of saccharifying enzyme, and react at 60°C for 30 min;
[0048] (4) Inactivate the enzyme in a boiling water bath for 5 min, cool to room temperature, centrifuge for 20 min (4000 rpm), take the supernatant after centrifugation, wash the precipitate with distilled water and retain the washing solution;
[0049] (5) Store at 4°C, concentrate the supernatant and washing solution, measure the volume, add anhydrous ethanol for alcohol precipitation (four times the volume, 4h), centrifuge for 15min (4000rpm), wash the precipitate twice with anhydrous ethanol, freeze-dry the precipitate, crush and sieve (100 mesh), obtain water-soluble dietary fiber, and store at 4°C.
[0050] In the step I, the seabuckthorn seed skin residue raw material is defatted seabuckthorn seed residue, and the mass ratio of the seabuckthorn seed residue to distilled water is 1:9.71.
[0051] In the step I, the mass ratio of alkaline protease to seabuckthorn seed skin residue is 1:35, the reaction pH is 9.0, the reaction time is 30 minutes, and the reaction temperature is 50°C.
[0052] In the step II, the mass ratio of α-amylase to seabuckthorn seed skin residue is 1:12.32, the reaction pH is 4.0, the reaction time is 30 minutes, and the reaction temperature is 60°C.
[0053] In the step III, the mass ratio of saccharifying enzyme to seabuckthorn seed skin residue is 1:29.17, the pH value is 6.5, the static reaction time is 30 minutes, and the reaction temperature is 60°C.
[0054] In step IV, the centrifugal operation has a centrifugal speed of 4000 rpm and a centrifugal time of 20 min.
[0055] Embodiment 2:
[0056] like Figure 1-Figure 5 As shown, a method for extracting water-soluble dietary fiber from seabuckthorn seed peel residue:
[0057] The structural properties of water-soluble dietary fiber were measured by Fourier infrared spectroscopy, X-ray diffraction, and scanning electron microscopy;
[0058] (1) Fourier transform infrared spectroscopy
[0059] Weigh 2 mg of SDF and IDF respectively, mix with an appropriate amount of KBr powder, grind evenly and press into tablets. Put the prepared tablets into an infrared spectrometer for scanning in the range of 400-4000 cm-1, and analyze the collected infrared absorption spectrum using Nexus system software.
[0060] Figure 1 As shown, at 3274cm -1A broad peak caused by OH stretching vibration is shown at 1631cm -1 The absorption peak at 1039cm -1 The nearby ones correspond to the stretching vibration of C=O(-COC) in cellulose and hemicellulose, which are infrared absorption marks unique to polysaccharides, proving that SDF contains the basic framework of sugar structure;
[0061] (2) X-ray diffraction measurement
[0062] X-ray diffractometer was used to measure the diffraction patterns of SDF before and after steam explosion modification. A copper target was used, the tube voltage and tube current were set to 40 kV and 40 mA, the diffraction angle was set to 2θ, and the scanning range was 5°-60°.
[0063] X-ray diffraction can be used to determine the crystal structure of dietary fiber, and different crystal structures correspond to different diffraction angles. Figure 2 The results in show the typical cellulose forms in SDF, with relative peaks of different intensities. It is reported that due to the presence of cellulose, DF is mainly composed of crystalline and non-crystalline regions, and the non-crystalline region is composed of hemicellulose and lignin. The strong peaks of SDF near 22, 32, and 45° in the figure are the main diffraction peaks, indicating that SDF has typical cellulose crystals. In addition, the diffraction peaks of SDF are weak. The reason for this phenomenon is that the disordered amorphous hemicellulose attached to the surface of cellulose microfibrils is preferentially hydrolyzed during alkali treatment during enzymatic extraction;
[0064] (3) Scanning electron microscopy measurement
[0065] A small amount of sample was placed on the conductive glue, gold-plated using a vacuum sputtering device, and then scanned and photographed under an electron microscope at 2000 times and 5000 times magnification.
[0066] Figure 3 The surface of SDF in A presents a smooth and flat sheet structure as a whole, with some branched structures and relatively uniform, rounded spherical particles in Figure B. This shape helps to increase its contact area with water and promote dissolution. It has strong hydrophilicity. It may also be that the polymerization and cross-linking between dietary fiber groups may also lead to the formation of spherical morphology.
[0067] The functional test of water-soluble dietary fiber is carried out, and the test items are: water holding capacity, oil holding capacity, swelling capacity and water binding capacity;
[0068] Water holding capacity determination
[0069] Accurately weigh 0.5g (m1) sample into a 20mL clean centrifuge tube, add 10mL deionized water, shake and mix at room temperature for 30min, let stand for 24h, centrifuge at 5000rpm / min for 10min, carefully aspirate the supernatant, absorb excess water with filter paper, weigh the sample after water absorption (m2), and calculate the water holding capacity according to the following formula. Repeat three times for each sample and take the average value.
[0070] Water holding capacity (g / g) = (m2-m1) / m1
[0071] Oil holding capacity determination
[0072] Accurately weigh 0.5g (m1) sample into a 20mL clean centrifuge tube, add 10mL corn oil, shake and mix at room temperature for 30min, let stand for 24h, centrifuge at 5000rpm / min for 20min, carefully pour off the upper layer of oil, absorb excess oil with filter paper, weigh the mass of the sample after oil retention (m2), and calculate the oil retention capacity according to the following formula. Repeat three times for each sample and take the average value.
[0073] Oil holding capacity (g / g) = (m2-m1) / m1
[0074] Determination of expansion force
[0075] Accurately weigh 0.5g (m1) of sample into a dry measuring cylinder, read its initial volume (V1), add 10mL of deionized water, stir and mix at room temperature for 30min, let stand for 24h, read its volume after free expansion (V2), and calculate the expansion force according to the following formula. Repeat three times for each sample and take the average value.
[0076] Expansion force (mL / g) = (V2-V1) / m1
[0077] Hydraulic binding test
[0078] For the determination of combined water, take 0.1 g (m3) of sample into a centrifuge tube to which 10 mL of distilled water has been added, shake at room temperature for 1 hour, take the precipitate, let it stand at room temperature for 1 hour, then weigh it and record it as m1. Then place the precipitate in a constant temperature drying oven for drying, weigh it and record it as m2.
[0079] Bound water (g / g) = (m1-m2) / m3
[0080] Figure 4 The results are the effects of the hydration properties of seabuckthorn seed residue SDF. Water holding capacity reflects the ability of dietary fiber to retain water after compression or centrifugation, and can reflect the effect of dietary fiber on intestinal peristalsis. Figure 4 It can be seen that the water holding capacity of SDF is poor, which is related to its loose structure.
[0081] The swelling force of SDF is (0.74±0.04) mL / g, which is attributed to its rounded particles, which increase the contact interface with water and significantly enhance the swelling performance.
[0082] Embodiment three:
[0083] like Figure 1-Figure 5 As shown in, an application of extracting water-soluble dietary fiber from seabuckthorn seed skin residue:
[0084] It is used in the regulation of fat metabolism and the reduction of serum cholesterol;
[0085] Used in improving food quality and preventing intestinal diseases.
[0086] In terms of fat metabolism regulation and serum cholesterol reduction, oil holding capacity plays a key role. Cellulose with high oil holding capacity can effectively slow down the absorption rate of fat. Specifically, the SDF is (1.33±0.30) g / g.
[0087] In terms of improving food quality and preventing intestinal diseases, dietary fiber with excellent water binding capacity is particularly important. In this regard, SDF showed the best performance, with a water binding capacity of up to (1.86±0.20) g / g. As a direct reflection of the interaction between dietary fiber and water, its strength is mainly limited by the structural characteristics of the components. This further confirms that enzyme treatment makes the SDF surface have a large number of group polymerization and cross-linking, making it easier to bind with water.
[0088] Comprehensive evaluation showed that SDF performed well in binding hydraulics, which was due to the rounded spherical particles formed during the enzyme treatment, which greatly increased the contact area with water and promoted the dissolution process.
[0089] Cholesterol adsorption capacity
[0090] Take 10.0 mg of cholesterol standard and dilute to 100 mL volumetric flask with glacial acetic acid to prepare cholesterol standard solution. Take 0, 0.16, 0.32, 0.48, 0.64, and 0.80 mL of 0.1 mg / mL cholesterol standard solution respectively, add glacial acetic acid to 0.80 mL and mix. Add 0.4 mL of 1.0 mg / mL o-phthalaldehyde solution prepared with glacial acetic acid and 8 mL of mixed acid, mix glacial acetic acid and concentrated sulfuric acid in a volume ratio of 1:1, and let stand at room temperature for 10 minutes after mixing. Measure the absorbance at a wavelength of 550 nm and draw a standard curve. The cholesterol standard curve was measured: y=0.1216x-0.0917, R 2 =0.9909.
[0091] Sample determination: The yolk of fresh eggs was mixed with distilled water in a ratio of 1:9 (mixed solution), 0.1 g of sample was mixed evenly with 5 mL of the mixed solution, the pH was adjusted to 2.0 and 7.0 respectively, and then reacted in a shaker at 37°C for 2 h. Then centrifuged at 10000 r / min for 10 min, 0.1 mL of supernatant was taken, 0.7 mL of glacial acetic acid, 0.4 mL of o-phthalaldehyde solution and 8.0 mL of mixed acid were added, mixed and placed for 10 min, and 200 μL of sample solution was taken to measure the absorbance at 550 nm. The control was the egg yolk mixed solution without sample, and the CAC was calculated as shown in formula (5):
[0092] Cholesterol adsorption capacity (mg / g) = (m2-m3) / m1
[0093] Wherein, m2 is the cholesterol content in the unsampled solution (mg), m3 is the cholesterol content in the sampled solution (mg), and m1 is the sample mass (g).
[0094] The cholesterol adsorption capacity of SDF was measured under pH 2.0 (simulated stomach) and pH 7.0 (simulated intestine) conditions. Figure 5 As shown. Figure 5 It can be seen that when simulating the gastric environment, the adsorption capacity of cholesterol is SDF (4.5±0.34) mg / g. When simulating the intestinal environment, the cholesterol adsorption capacity of SDF is (6.25±0.31) mg / g. The above research results found that the cholesterol adsorption capacity of the same SDF in simulated intestine (pH7.0) is significantly greater than that in simulated stomach (pH2.0). Excessive intake of cholesterol is considered to be one of the potential risk factors for chronic diseases such as hyperlipidemia, atherosclerosis and even cardiovascular diseases. The uniqueness of dietary fiber is that its surface is rich in active groups that can produce a chelating effect with cholesterol. This mechanism effectively curbs the conversion process of cholesterol to bile acid, and thus shows a positive effect in reducing cholesterol accumulation in the liver.
[0095] Glucose adsorption capacity
[0096] Take 100.0 mg of anhydrous glucose standard in a 100 mL volumetric flask and dilute to volume with distilled water to prepare a glucose standard solution. Add 0.0, 0.1, 0.2, 0.4, 0.8, and 1.0 mL of glucose standard solution to a 15 mL centrifuge tube and add distilled water to make up to 1.0 mL. Add 2.0 mL of DNS reagent to the centrifuge tube, heat in a boiling water bath for 2 minutes, cool, and dilute to 15 mL. Measure the absorbance at a wavelength of 540 nm to draw a standard curve. The glucose standard curve was measured as follows: y = 1.1068x + 0.0094, R 2 =0.9988.
[0097] Sample determination: Take 0.1g sample and mix with 10mL 100mg / mL glucose solution. After 6h in 37℃ water bath, centrifuge at 4000r / min for 20min, take the supernatant, and colorimetric at 540nm using DNS method. The control is glucose solution without dietary fiber. The calculation formula is as follows:
[0098] GAC=(m1-m2) / m3
[0099] Where: m1, original mass of glucose solution (mg); m2, mass of supernatant glucose solution after centrifugation (mg); m3, mass of sample (g).
[0100] Depend on Figure 5 It can be seen that SDF is (45.48±1.06) mg / g. The surface of the SDF dietary fiber treated with enzymes forms a porous layer structure, which promotes the dietary fiber to adsorb more glucose molecules. The smooth spherical structure causes more chemical groups to be exposed, and the affinity between SDF and glucose molecules is enhanced, all of which promote the improvement of the glucose adsorption capacity of dietary fiber.
[0101] The present invention adopts a composite enzyme method to extract seabuckthorn seed skin residue SDF, and explores the structural properties and functional characteristics. The results show that the surface of SDF presents a smooth and flat flaky structure as a whole, and some branched structures and relatively uniform, rounded spherical particles appear, and the structure of SDF is looser. Infrared spectroscopy shows that SDF has cellulose spectral characteristics, all of which are cellulose I-type diffraction peaks. SDF has the best binding water power (1.86±0.20) g / g, cholesterol adsorption capacity (6.25±0.31) mg / g and glucose adsorption capacity (45.48±1.06) mg / g. The results of the present invention can promote the comprehensive utilization of seabuckthorn by-products.
[0102] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A method for extracting water-soluble dietary fiber from seabuckthorn seed husk residue, characterized in that: The following steps are involved: I. Put 175-200g seabuckthorn seed skin residue into a beaker, add 1700-1945ml distilled water and adjust the pH to 9.0, add 5.0g alkaline protease, and react at 50°C for 30min; II. Following step I, adjust the pH to 6.5, add 14.2 g of α-amylase, and react at 60°C for 30 min; III. Following step II, adjust the pH to 4.0, add 6.0 g of saccharifying enzyme, and react at 60°C for 30 min; IV. Following step III, inactivate the enzyme in a boiling water bath for 5 min, cool to room temperature, centrifuge, take the supernatant, wash the precipitate with distilled water and retain the washing solution; V. Following step IV, the washing solution was stored at 4°C, the supernatant and washing solution were concentrated, the volume was measured, four times the volume of anhydrous ethanol was added for alcohol precipitation for 4 hours, and then centrifuged at 4000 rpm for 15 minutes, the precipitate was washed twice with anhydrous ethanol, the precipitate was freeze-dried, crushed through a 100-mesh sieve, and water-soluble dietary fiber was obtained, which was then stored at 4°C.
2. The method for extracting water-soluble dietary fiber from seabuckthorn seed husk residue according to claim 1, characterized in that: In the step I, the seabuckthorn seed skin residue raw material is defatted seabuckthorn seed residue, and the mass ratio of the seabuckthorn seed residue to distilled water is 1:9.
71.
3. The method for extracting water-soluble dietary fiber from seabuckthorn seed husk residue according to claim 1, characterized in that: In the step I, the mass ratio of alkaline protease to seabuckthorn seed skin residue is 1:35, the reaction pH is 9.0, the reaction time is 30 minutes, and the reaction temperature is 50°C.
4. The method for extracting water-soluble dietary fiber from seabuckthorn seed husk residue according to claim 1, characterized in that: In the step II, the mass ratio of α-amylase to seabuckthorn seed skin residue is 1:12.32, the reaction pH is 4.0, the reaction time is 30 minutes, and the reaction temperature is 60°C.
5. The method for extracting water-soluble dietary fiber from seabuckthorn seed husk residue according to claim 1, characterized in that: In the step III, the mass ratio of saccharifying enzyme to seabuckthorn seed skin residue is 1:29.17, the pH value is 6.5, the static reaction time is 30 minutes, and the reaction temperature is 60°C.
6. The method for extracting water-soluble dietary fiber from seabuckthorn seed husk residue according to claim 1, characterized in that: In step IV, the centrifugal operation has a centrifugal speed of 4000 rpm and a centrifugal time of 20 min.
7. The method for extracting water-soluble dietary fiber from seabuckthorn seed husk residue according to claim 1, characterized in that: The structural properties of water-soluble dietary fiber were measured by Fourier infrared spectroscopy, X-ray diffraction, and scanning electron microscopy; The functional test of water-soluble dietary fiber is carried out, and the test items are: water holding capacity, oil holding capacity, swelling capacity and water binding capacity.
8. The use of extracting water-soluble dietary fiber from seabuckthorn seed husk residue according to claim 1, characterized in that: It is used in the regulation of fat metabolism and the reduction of serum cholesterol; Used in improving food quality and preventing intestinal diseases.