Preparation method and application of marine algae composition with anti-wrinkle, anti-inflammatory and repairing effects
The method of physical and enzymatic treatment of marine algae enhances the extraction of active ingredients, addressing inefficiencies in existing methods to achieve superior anti-wrinkle, anti-inflammatory, and anti-aging skin care product performance.
Patent Information
- Application Number
- CN202411571615.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-06
- Publication Date
- 2025-01-03
AI Technical Summary
Existing methods for preparing marine algae compositions for skin care products are inefficient due to long fermentation times and high sugar consumption, leading to low effective ingredient content.
A method involving the physical treatment and enzymatic hydrolysis of brown, red, and Chlorella algae to obtain extracts, combined with specific auxiliary materials, to enhance the release and retention of active ingredients, including the use of diatomaceous earth for brown algae extraction and a mixed enzyme for red algae hydrolysis.
The method improves the extraction of effective ingredients, resulting in enhanced anti-wrinkle, anti-inflammatory, and anti-aging effects through increased type I collagen expression, MMP-1 inhibition, and hyaluronic acid up-regulation.
Smart Images
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Abstract
Description
Technical Field
[0001] The invention relates to the technical field of skin care products, in particular to a preparation method and application of a marine algae composition with anti-wrinkle, anti-inflammatory and repairing effects. Background Art
[0002] Recent studies have found that marine algae are rich in nutrients, such as protein, polysaccharides, minerals, trace elements, etc. The polysaccharide content is relatively high and has certain efficacy. It is widely used in anti-wrinkle, anti-inflammatory, firming, repair and other functional skin care products.
[0003] It is well known that although polysaccharides can be absorbed through the skin, the polysaccharide molecules absorbed by the skin must be smaller than 5000 Daltons, and the polysaccharide molecules contained in marine algae are usually relatively large and difficult to penetrate the skin.
[0004] Improvements have been made in the industry. For example, a Chinese patent discloses an algae fermentation composition, a skin care product and its application in skin care. The application publication number is CN 117959236A, which records that "the algae fermentation composition is obtained by fermenting algae with probiotics; wherein the probiotics include Saccharomyces cerevisiae, Lactobacillus plantarum and Lactobacillus acidophilus; the algae include Gracilaria rubrum (red algae), Spirulina maxima (blue algae), Chlorella vulgaris (green algae) and Ascophyllum nodosum (brown algae). The present invention uses three probiotics to ferment the mixed seaweed three times, thereby effectively improving the activity of the algae active substances and promoting the release of nutritional active ingredients such as algae oligosaccharides and polyphenols. At the same time, it has been verified by experiments that it has the effects of antioxidant, anti-inflammatory, antibacterial and telomere protection and anti-aging."
[0005] The shortcomings of this technical solution are: first, the fermentation takes a long time; second, using algae as a fermentation substrate will inevitably consume a large amount of sugars carried by the algae, which easily leads to a low content of effective ingredients in the extract. Summary of the invention
[0006] In view of the deficiencies in the prior art, one of the objects of the present invention is to provide a method for preparing a marine algae composition with anti-wrinkle, anti-inflammatory and repairing effects. A second object of the present invention is to provide a skin care product containing the marine algae composition with anti-wrinkle, anti-inflammatory and repairing effects.
[0007] One of the purposes of the present invention is achieved through the following technical solutions:
[0008] A method for preparing a marine algae composition with anti-wrinkle, anti-inflammatory and repairing effects comprises the following steps:
[0009] S1 crushes the brown algae and extracts to obtain a brown algae extract;
[0010] S2 hydrolyzes and extracts the red algae to obtain a red algae extract;
[0011] S3 cultivates Chlorella and extracts it to obtain Chlorella extract;
[0012] S4: weigh 10-20% of brown algae extract, 5-10% of red algae extract, 2-5% of chlorella extract by weight, and the remainder as auxiliary materials, and mix them evenly to obtain a composition.
[0013] Specifically, the method of crushing brown algae and extracting to obtain brown algae extract comprises:
[0014] Firstly, brown algae, diatomaceous earth and 50wt% ethanol aqueous solution are mixed in a weight ratio of 100:1-5:900; then, the wall is broken at a speed of 18000-40000r / min for 8 minutes; the brown algae is placed in a dark room at 25-30℃ for extraction for 10-15 hours, and vacuum concentrated in an environment of 40-50℃ until the ethanol content is less than 15wt%, and finally, the brown algae extract is obtained by air-drying at room temperature through a 100-200 mesh sieve.
[0015] Specifically, the brown algae is at least one of Sargassum, Copper Algae, Laminaria, Undaria, Sargassum or Salmonella.
[0016] Specifically, the method of hydrolyzing red algae and extracting to obtain the red algae extract comprises:
[0017] First, the red algae is mixed with a 10wt% acetic acid aqueous solution in a weight ratio of 1:7-12; then the wall is broken at a speed of 18000-40000r / min for 8 minutes to obtain a wall-breaking liquid; the wall-breaking liquid is heated to 80-90°C and stirred at a speed of 60-200r / min for 5-8h, and then the pH of the wall-breaking liquid is adjusted to 10 with a sodium hydroxide solution, and a mixed enzyme is added at 5-8% of the weight of the red algae, and hydrolyzed at 37-55°C for 2-4h to obtain a hydrolyzate; finally, the hydrolyzate is filtered, the enzyme is inactivated, and dried to a water content of less than 15wt%, which is a red algae extract.
[0018] Specifically, the red algae is at least one of agar-agar, Gracilaria or Porphyra.
[0019] Specifically, the mixed enzyme is obtained by mixing cellulase, pectinase, xylanase and clam (Meretrix linnae-us) digestive enzyme in a weight ratio of 5:4:3:1:3-8.
[0020] Specifically, the method of culturing Chlorella and extracting the Chlorella extract comprises:
[0021] First, the chlorella is placed in a BG11 medium and cultured until OD650nm=0.6 to obtain a culture solution, and then a sodium hydroxide solution is used to adjust the pH to 14, and the culture solution is stirred at a speed of 30-120r / min for 30-50min; then citric acid is used to adjust the pH to 10, and the cell wall is broken at a speed of 18000-40000r / min for 8min to obtain a cell wall liquid, and the cell wall liquid is stirred at a speed of 60-200r / min for 5-8h; finally, the chlorella extract is filtered and sterilized, and dried to a water content of less than 15wt%. The filtration and sterilization is performed by filtering with a 200-mesh screen and a 0.22μm bacteria filter in sequence.
[0022] Specifically, the auxiliary material is prepared by mixing pure water, glycerol and monoglyceride in a weight ratio of 10-15:5:2.
[0023] The second object of the present invention is achieved by the following technical solutions:
[0024] A skin care product containing a marine algae composition with anti-wrinkle, anti-inflammatory and repairing effects, comprising the following components in percentage by weight:
[0025] Hydroxypropyl starch 10-15%, beeswax 1-3%, stearic acid 1-4%, combination 1-5%, and the balance is deionized water.
[0026] The beneficial effects of the present invention are as follows: firstly, the algae are treated by physical means and enzymes to improve the release of effective ingredients and reduce the consumption of polysaccharides; in addition, diatomaceous earth is combined with brown algae wall breaking extraction to further improve the extraction of effective active ingredients; finally, the brown algae extract, the hydrolyzed red algae extract and the chlorella extract are combined to produce excellent anti-wrinkle, anti-inflammatory and anti-aging effects. DETAILED DESCRIPTION
[0027] The following is further described in conjunction with specific implementation methods:
[0028] Example 1
[0029] The preparation of a marine algae composition having anti-wrinkle, anti-inflammatory and repairing effects comprises the following steps:
[0030] Crushing brown algae and extracting to obtain brown algae extract:
[0031] First, kelp, diatomaceous earth and 50wt% ethanol aqueous solution are mixed in a weight ratio of 100:1.5:900; then, the wall is broken at a speed of 18000r / min for 8min; the mixture is placed in a dark room at 25°C for extraction for 11h, and vacuum concentrated in an environment of 41°C to an ethanol content of less than 15wt%, and finally, the mixture is passed through a 100-mesh sieve and air-dried at room temperature (in this embodiment, the mixture is air-dried to a water content of 13wt%, the same below) to obtain a brown algae extract.
[0032] Hydrolyze and extract red algae to obtain red algae extract:
[0033] First, the laver is mixed with a 10wt% acetic acid aqueous solution in a weight ratio of 1:8; then the laver is broken at a speed of 18000r / min for 8min to obtain a broken liquid; the broken liquid is heated to 80°C and stirred at a speed of 70r / min for 5h, and then the pH of the broken liquid is adjusted to 10 with a sodium hydroxide solution, and a mixed enzyme is added at 5% of the weight of the red algae, wherein the mixed enzyme is obtained by mixing cellulase, pectinase, xylanase and clam digestive enzyme in a weight ratio of 5:4:3:1:3, and the hydrolyzate is obtained by hydrolysis at 40°C for 2h; finally, the hydrolyzate is filtered, the enzyme is inactivated and dried to a water content of 13wt%, which is the red algae extract.
[0034] Cultivate and extract Chlorella to obtain Chlorella extract:
[0035] First, Chlorella is placed in a BG11 culture medium and cultured until OD650nm=0.6 to obtain a culture solution, and then a sodium hydroxide solution is used to adjust the pH to 14, and the solution is stirred at a speed of 50 r / min for 30 minutes; then citric acid is used to adjust the pH to 10, and the cell wall is broken at a speed of 18000 r / min for 8 minutes to obtain a cell wall liquid, and the cell wall liquid is stirred at a speed of 80 r / min for 5 hours; finally, filtration and sterilization (the filtration and sterilization adopts a 200-mesh screen and a 0.22 μm bacteria filter to filter in sequence, the same below), and drying to a water content of 13wt%, is the Chlorella extract.
[0036] Preparation of auxiliary materials:
[0037] The auxiliary material is prepared by mixing pure water, glycerol and monoglyceride in a weight ratio of 10:5:2.
[0038] 10% of brown algae extract, 5% of red algae extract, 2% of chlorella extract were weighed by weight, and the remainder was used as auxiliary materials, and mixed to obtain a composition.
[0039] Preparation of skin care products:
[0040] Weigh the following components in weight percentage:
[0041] 10% hydroxypropyl starch, 1% beeswax, 1% stearic acid, 2% combination, and the balance is deionized water.
[0042] The speed is 10000r / min to emulsify evenly to obtain skin care products.
[0043] Example 2
[0044] The preparation of a marine algae composition having anti-wrinkle, anti-inflammatory and repairing effects comprises the following steps:
[0045] Crushing brown algae and extracting to obtain brown algae extract:
[0046] First, mix kelp with diatomaceous earth and 50wt% ethanol aqueous solution in a weight ratio of 100:5:900; then break the wall at a speed of 40000r / min for 8min; place it in a dark room at 30℃ for extraction for 15h, vacuum concentrate it in a 49℃ environment until the ethanol content is less than 15wt%, and finally pass it through a 200-mesh sieve and air-dry it at room temperature to obtain the brown algae extract.
[0047] Hydrolyze and extract red algae to obtain red algae extract:
[0048] First, agar is mixed with a 10wt% acetic acid aqueous solution in a weight ratio of 1:12; then, the wall is broken at a speed of 40000r / min for 8min to obtain a wall-breaking liquid; the wall-breaking liquid is heated to 90°C and stirred at a speed of 200r / min for 8h, and then the pH of the wall-breaking liquid is adjusted to 10 with a sodium hydroxide solution, and a mixed enzyme is added at 8% of the weight of the red algae, wherein the mixed enzyme is obtained by mixing cellulase, pectinase, xylanase and clam digestive enzyme in a weight ratio of 5:4:3:1:8, and the hydrolyzate is obtained by hydrolysis at 55°C for 4h; finally, the hydrolyzate is filtered, the enzyme is inactivated and dried to a water content of 13wt%, which is the red algae extract.
[0049] Cultivate and extract Chlorella to obtain Chlorella extract:
[0050] First, Chlorella is placed in a BG11 culture medium and cultured until OD650nm=0.6 to obtain a culture solution, and then a sodium hydroxide solution is used to adjust the pH to 14, and the solution is stirred at a speed of 120 r / min for 50 minutes; then, citric acid is used to adjust the pH to 10, and the cell wall is broken at a speed of 40000 r / min for 8 minutes to obtain a cell wall liquid, and the cell wall liquid is stirred at a speed of 200 r / min for 8 hours; finally, filtration and sterilization are carried out, and the solution is dried to a water content of 13wt%, which is a Chlorella extract.
[0051] Preparation of auxiliary materials:
[0052] The auxiliary material is prepared by mixing pure water, glycerol and monoglyceride in a weight ratio of 15:5:2.
[0053] Weigh 20% of brown algae extract, 10% of red algae extract, and 5% of chlorella extract by weight, and mix the remainder as auxiliary materials to obtain a composition.
[0054] Preparation of skin care products:
[0055] Weigh the following components in weight percentage:
[0056] 15% hydroxypropyl starch, 3% beeswax, 4% stearic acid, 5% combination, and the balance is deionized water.
[0057] The speed is 10000r / min to emulsify evenly to obtain skin care products.
[0058] Example 3
[0059] The preparation of a marine algae composition having anti-wrinkle, anti-inflammatory and repairing effects comprises the following steps:
[0060] Crushing brown algae and extracting to obtain brown algae extract:
[0061] First, kelp was mixed with diatomaceous earth and 50wt% ethanol aqueous solution in a weight ratio of 100:3:900; then, the wall was broken at a speed of 30000r / min for 8min; the mixture was placed in a dark room at 27°C for extraction for 13h, vacuum concentrated in a 46°C environment until the ethanol content was less than 15wt%, and finally, the mixture was passed through a 150-mesh sieve and air-dried at room temperature to obtain the brown algae extract.
[0062] Hydrolyze and extract red algae to obtain red algae extract:
[0063] First, the laver is mixed with a 10wt% acetic acid aqueous solution in a weight ratio of 1:10; then the laver is broken at a speed of 30000r / min for 8min to obtain a broken liquid; the broken liquid is heated to 85°C and stirred at a speed of 100r / min for 6h, and then the pH of the broken liquid is adjusted to 10 with a sodium hydroxide solution, and a mixed enzyme is added at 7% of the weight of the red algae, wherein the mixed enzyme is obtained by mixing cellulase, pectinase, xylanase and clam digestive enzyme in a weight ratio of 5:4:3:1:5, and the hydrolyzate is obtained by hydrolysis at 42°C for 3h; finally, the hydrolyzate is filtered, the enzyme is inactivated and dried to a water content of 13wt%, which is the red algae extract.
[0064] Cultivate and extract Chlorella to obtain Chlorella extract:
[0065] First, Chlorella is placed in a BG11 culture medium and cultured until OD650nm=0.6 to obtain a culture solution, and then a sodium hydroxide solution is used to adjust the pH to 14, and the solution is stirred at 70 r / min for 40 minutes; then, citric acid is used to adjust the pH to 10, and the cell wall is broken at 30000 r / min for 8 minutes to obtain a cell wall liquid, and the cell wall liquid is stirred at 100 r / min for 7 hours; finally, the solution is filtered and sterilized, and dried to a water content of 13wt%, to obtain a Chlorella extract.
[0066] Preparation of auxiliary materials:
[0067] The auxiliary material is prepared by mixing pure water, glycerol and monoglyceride in a weight ratio of 13:5:2.
[0068] 15% of brown algae extract, 7% of red algae extract, 3% of chlorella extract were weighed by weight, and the remainder was used as auxiliary materials, and mixed to obtain a composition.
[0069] Preparation of skin care products:
[0070] Weigh the following components in weight percentage:
[0071] Hydroxypropyl starch 12%, beeswax 2%, stearic acid 3%, combination 3%, and the balance is deionized water.
[0072] The speed is 10000r / min to emulsify evenly to obtain skin care products.
[0073] Comparative Example 1
[0074] The diatomaceous earth was replaced by an equal amount of activated carbon powder, and the remaining steps were the same as in Example 3.
[0075] Comparative Example 2
[0076] The timing of adding diatomaceous earth was changed to after the kelp (brown algae) wall was broken, and the remaining steps were the same as in Example 3.
[0077] Comparative Example 3
[0078] The clam digestive enzyme was replaced by an equal amount of papain, and the remaining steps were the same as in Example 3.
[0079] Efficacy test
[0080] 1. Experimental purpose: To verify that the composition obtained by the present invention has better anti-wrinkle, anti-aging and repair effects.
[0081] 2. Experimental method: The enhancement rate of type I collagen expression, the inhibition rate of MMP-1 (matrix metalloproteinase) and the up-regulation rate of hyaluronic acid expression of the compositions obtained in Examples 1-3 and Comparative Examples 1-3 were respectively determined.
[0082] 2-1. Method for determining the increase rate of type I collagen expression
[0083] 3T3 mouse fibroblasts (purchased from the cell bank of the Chinese Academy of Sciences) were diluted with cell culture medium and cultured to a density of 50%, inoculated into 96-well plates, with a liquid volume of 200 μL per well, and placed in a CO2 incubator for 24 hours. The culture medium in the 96-well plate was discarded and the drug administration operation was carried out. A culture medium (composition content of 1.25 mg / mL) containing the test substance (a composition obtained in one of Examples 1-3 or Comparative Examples 1-3) was added to the test well, and normal cell culture medium was added to the blank / solvent control well, 200 μL per well. After the administration is completed, the 96-well plate was placed in a CO2 incubator for 24 hours. After the incubation and culture, 200 μL of cell culture supernatant was collected in a 1.5 mL sterile centrifuge tube and placed in a -80°C ultra-low temperature refrigerator for freezing. The test was performed according to the instruction manual of the mouse type I collagen enzyme-linked immunosorbent assay kit. The increase rate of type I collagen expression was calculated.
[0084] 2-2. Method for determining the inhibition rate of MMP-1 (matrix metalloproteinase)
[0085] HaCaT cells (purchased from Shanghai Jinyuan Biotechnology Co., Ltd.) were inoculated in a 96-well plate and cultured to a density of 80%. A blank group, a model group, and an experimental group were set up, and 6 replicate wells were set up in each group to take the average. The blank group was not irradiated with UVB, and 10 μL of culture medium was added for treatment for 2 hours, and then cultured at 37°C for 24 hours before use in the experiment. The HaCaT cells in the model group were treated with 10 μL of culture medium for 2 hours, and then irradiated with 60 mJ / cm2 of UVB, and cultured at 37°C for 24 hours before use in the experiment. The HaCaT cells in the experimental group were treated with 10 μL of culture medium (the culture medium contained 0.5 mg / mL of the composition) for 2 hours, and then irradiated with 60 mJ / cm2 of UVB, and cultured at 37°C for 24 hours before use in the experiment. Centrifuge for 10 minutes at 4°C and 12000 r / min, and collect the supernatant. The protein in the supernatant was quantified using a BCA protein quantification kit to determine the final concentration of 10 μg / mL. The expression level of secretory protein MMP-1 was determined according to the instructions of the enzyme-linked immunosorbent assay (ELISA) kit, and the inhibition rate of MMP-1 (matrix metalloproteinase) was calculated.
[0086] 2-3. Method for determining the up-regulation rate of hyaluronic acid expression
[0087] A9 mouse subcutaneous connective tissue cells (purchased from Henan Industrial Microbial Strain Engineering Technology Research Center) were diluted with cell culture medium and cultured to a density of 50%, inoculated into 96-well plates, and the liquid volume per well was 200 μL, and placed in a CO2 incubator for 24 hours. The culture medium in the 96-well plate was discarded and the administration operation was carried out. A culture medium (composition content of 1.25 mg / mL) containing the test substance (composition obtained in one of Examples 1-3 or Comparative Examples 1-3) was added to the test well, and normal cell culture medium was added to the blank / solvent control well, 200 μL per well. After the administration is completed, the 96-well plate is placed in a CO2 incubator for 24 hours. After the incubation and culture are completed, 200 μL of cell culture supernatant was collected in a 1.5 mL sterile centrifuge tube and placed in a -80°C ultra-low temperature refrigerator for freezing and storage. Detection was performed according to the instruction manual of the mouse hyaluronic acid (HA) ELISA kit. Calculate the upregulation rate of hyaluronic acid expression.
[0088] 3. Experimental results:
[0089] 3-1. The improvement rate of type I collagen expression is shown in Table 1;
[0090] Table 1 The improvement rate of type I collagen expression
[0091]
[0092] 3-2, the inhibition rate of MMP-1 (matrix metalloproteinase), see Table 2;
[0093] Table 2 Inhibition rate of MMP-1 (matrix metalloproteinase)
[0094]
[0095] 3-3. The up-regulation rate of hyaluronic acid expression is shown in Table 3;
[0096] Table 3 Upregulation rate of hyaluronic acid expression
[0097]
[0098] 4. Data analysis: From Table 1, it can be seen that the present invention has an expression increase rate of type I collagen of more than 50%, and has excellent anti-wrinkle effect. In addition, when diatomaceous earth is lacking in the extraction process of brown algae or the timing of input is wrong, the expression increase rate of type I collagen will be significantly reduced. From Table 2, it can be seen that the inhibition rate of MMP-1 (matrix metalloproteinase) of the present invention is greater than 8%, which has excellent anti-aging effect. In addition, when clam digestive enzymes are lacking in the hydrolysis process of red algae, the inhibition rate of MMP-1 (matrix metalloproteinase) will be greatly reduced. From Table 3, it can be seen that the expression increase rate of hyaluronic acid of the present invention is greater than 7%, which has excellent repair effect. In addition, when diatomaceous earth is lacking in the extraction process of brown algae or the timing of input is wrong, the expression increase rate of hyaluronic acid will be significantly reduced. In summary, the compatibility of the three marine algae has anti-wrinkle, anti-aging and repair effects, and it can be seen from experiments that the effect is not linearly related to the dosage. The three must have a synergistic effect, and the effect can only be exerted under a specific preparation process and a specific dosage.
[0099] The above embodiments and descriptions are only for illustrating the principles and best embodiments of the present invention. Without departing from the spirit and scope of the present invention, the present invention may be subject to various changes and improvements, all of which fall within the scope of the present invention to be protected.
Claims
1. A method for preparing a marine algae composition with anti-wrinkle, anti-inflammatory and repairing effects, characterized in that: The following steps are involved: S1 crushes the brown algae and extracts to obtain a brown algae extract; S2 hydrolyzes and extracts the red algae to obtain a red algae extract; S3 cultivates Chlorella and extracts it to obtain Chlorella extract; S4: weigh 10-20% of brown algae extract, 5-10% of red algae extract, 2-5% of chlorella extract by weight, and the remainder as auxiliary materials, and mix them evenly to obtain a composition.
2. The method for preparing the marine algae composition with anti-wrinkle, anti-inflammatory and repairing effects according to claim 1, characterized in that: The method for crushing brown algae and extracting to obtain the brown algae extract comprises: Firstly, brown algae, diatomaceous earth and 50wt% ethanol aqueous solution are mixed in a weight ratio of 100:1-5:900; then, the wall is broken at a speed of 18000-40000r / min for 8 minutes; the brown algae is placed in a dark room at 25-30℃ for extraction for 10-15 hours, and vacuum concentrated in an environment of 40-50℃ until the ethanol content is less than 15wt%, and finally, the brown algae extract is obtained by air-drying at room temperature through a 100-200 mesh sieve.
3. The method for preparing the marine algae composition with anti-wrinkle, anti-inflammatory and repairing effects according to claim 2, characterized in that: The brown algae is at least one of Sargassum fusiformis, Copper algae, Laminaria japonica, Undaria pinnatifida, Sargassum sutchuenensis or Salvia thunbergii.
4. The method for preparing the marine algae composition with anti-wrinkle, anti-inflammatory and repairing effects according to claim 1, characterized in that: The method for hydrolyzing red algae and extracting to obtain the red algae extract comprises: First, the red algae is mixed with a 10wt% acetic acid aqueous solution in a weight ratio of 1:7-12; then the wall is broken at a speed of 18000-40000r / min for 8 minutes to obtain a wall-breaking liquid; the wall-breaking liquid is heated to 80-90°C and stirred at a speed of 60-200r / min for 5-8h, and then the pH of the wall-breaking liquid is adjusted to 10 with a sodium hydroxide solution, and a mixed enzyme is added at 5-8% of the weight of the red algae, and hydrolyzed at 37-55°C for 2-4h to obtain a hydrolyzate; finally, the hydrolyzate is filtered, the enzyme is inactivated, and dried to a water content of less than 15wt%, which is a red algae extract.
5. The method for preparing the marine algae composition with anti-wrinkle, anti-inflammatory and repairing effects according to claim 4, characterized in that: The red algae is at least one of agar-agar, gracilaria or laver.
6. The method for preparing the marine algae composition with anti-wrinkle, anti-inflammatory and repairing effects according to claim 4, characterized in that: The mixed enzyme is obtained by mixing cellulase, pectinase, xylanase and clam digestive enzyme in a weight ratio of 5:4:3:1:3-8.
7. The method for preparing the marine algae composition with anti-wrinkle, anti-inflammatory and repairing effects according to claim 1, characterized in that: The method for culturing Chlorella and extracting to obtain Chlorella extract comprises: First, the chlorella is placed in a BG11 culture medium and cultured until OD650nm=0.6 to obtain a culture solution, and then a sodium hydroxide solution is used to adjust the pH to 14, and the culture solution is stirred at a speed of 30-120r / min for 30-50min; then, citric acid is used to adjust the pH to 10, and the cell wall is broken at a speed of 18000-40000r / min for 8min to obtain a cell wall liquid, and the cell wall liquid is stirred at a speed of 60-200r / min for 5-8h; finally, filtration and sterilization are carried out, and drying is carried out until the water content is less than 15wt%, thereby obtaining a chlorella extract.
8. The method for preparing the marine algae composition with anti-wrinkle, anti-inflammatory and repairing effects according to claim 7, characterized in that: The filtration sterilization adopts 200 mesh screen and 0.22 μm bacteria filter to filter in sequence.
9. The method for preparing the marine algae composition with anti-wrinkle, anti-inflammatory and repairing effects according to claim 1, characterized in that: The auxiliary material is prepared by mixing pure water, glycerol and monoglyceride in a weight ratio of 10-15:5:
2.
10. A skin care product containing the composition according to claim 1, characterized in that: The composition includes the following weight percentages: Hydroxypropyl starch 10-15%, beeswax 1-3%, stearic acid 1-4%, combination 1-5%, and the balance is deionized water.