Microcapsule and preparation method thereof

Abstract

The invention provides a microcapsule and a preparation method thereof, and belongs to the field of microcapsules, the method comprises the following steps: step 1, soaking a microcapsule wall in a bonding component solution, and stirring under a heating condition to obtain a treated wall material; 2, adding a wall material into the microcapsule core material solution, stirring, filtering, and drying to obtain a core material-loaded wall material; and step 3, adding the wall material loaded with the core material into a first blocking component, uniformly mixing, adding a second blocking component, stirring under a heating condition, and then filtering and drying to obtain the microcapsule. According to the microcapsule, the treated cucurbit [8] uril is adopted as the microcapsule wall, the cavity of the microcapsule wall is filled with the bonding component, the bonding efficiency of the bonding component and the microcapsule core material is improved by adding the bonding component, and finally, the microcapsule core material is plugged in the cavity in a combined plugging agent mode, so that the microcapsule is prepared. Therefore, the overall stability and slow release speed of the microcapsule are improved.

Description

Technical Field

[0001] The invention belongs to the field of microcapsules, and in particular relates to a microcapsule and a preparation method thereof. Background Art

[0002] Microencapsulation technology is one of the high-tech technologies that has developed rapidly and is widely used. It has been developed since the 1930s. In the early days, microencapsulation technology was mainly used in the pharmaceutical industry (packaging drugs or active ingredients in gelatin capsules), and began to be used in the food industry in the mid-1980s. Microencapsulation technology is a technology that uses natural or synthetic polymer materials as capsule wall materials to embed and seal solid, liquid or gas substances (core materials) in a semipermeable or sealed capsule membrane to become a solid particle product. The application of microencapsulation technology in the food field can reduce the impact of environmental factors such as light, oxygen, system pH, metal ions, etc. on some environmentally sensitive food raw materials or nutrients, mask the bad flavor of food, change the physical state of food nutrients, and control release and enhance solubility.

[0003] Currently, common microcapsules use protein wall materials, such as soy protein, peanut protein isolate, etc. The above microcapsules have low antioxidant activity and embedding rate and are prone to adhesion. At the same time, the flavor of protein wall materials deteriorates after the microcapsules are re-dissolved and the stability is low. Summary of the invention

[0004] In view of this, the present invention aims to overcome the defects in the prior art and provides a microcapsule and a preparation method thereof.

[0005] To achieve the above object, the technical solution of the present invention is achieved as follows: The present invention provides a method for preparing microcapsules, comprising the following steps: Step 1 is to immerse the microcapsule wall in a bonding component solution and stir it under heating conditions to obtain a treated wall material; Step 2 is to add the treated wall material to the microcapsule core material solution, stir, filter and dry to obtain the wall material loaded with the core material; Step 3 is to add the wall material of the core material to the first plugging component, mix them evenly, then add the second plugging component thereto, stir under heating conditions, and then filter and dry to obtain the microcapsules.

[0006] Furthermore, the microcapsule wall in step 1 is prepared by the following steps: adding the eight-membered cucurbitacin ring to an acid solution, standing, filtering, and drying to obtain the microcapsule wall; the acid solution is a hydrochloric acid solution with a concentration of 1-10 mol / L; and the standing time is 1-10 days. The eight-membered cucurbitacin ring is used as a raw material, so that the microcapsule wall has a larger inner cavity, which can accommodate large core material molecules, and the structure is stable, thereby improving the sustained release performance of the microcapsule.

[0007] Furthermore, the bonding component solution in step 1 is an aqueous solution of chitosan and branched maltodextrin in a mass ratio of 1:10-100. Chitosan can improve the cross-linking effect of the entire bonding component by virtue of the branched structure of maltodextrin. Furthermore, the mass concentration of the aqueous solution containing branched maltodextrin is 30-60%; the molecular weight of the branched maltodextrin is less than or equal to 4500 g / mol; the deacetylation degree of the chitosan is 85-95%, and the molecular weight distribution is 50,000-200,000.

[0008] Furthermore, the temperature of the heating step in step 1 is 30-50° C., and the time of the stirring step is 1-10 hours.

[0009] Furthermore, the stirring step in step 2 is performed for 1 to 10 hours.

[0010] Furthermore, the first plugging component in step 3 is a euglena polysaccharide solution with a mass concentration of 1-20%, and the second plugging component is tannic acid; the solid-liquid ratio of the second plugging component to the first plugging component is 1g:1-10L. The second plugging component tannic acid improves the cross-linking property of the first plugging component euglena polysaccharide, and the combination of the two stably plugs the core material molecules in the cavity of the microcapsule wall, further improving the stability of the microcapsule.

[0011] Furthermore, the temperature of the heating step in step 3 is 50-80° C., and the time of the stirring step is 1-10 hours.

[0012] The invention provides a microcapsule prepared by the preparation method.

[0013] The present invention provides an application of a microcapsule, the application of the microcapsule in preparing a drug capsule preparation or food; and the application of the microcapsule in preparing milk powder.

[0014] Compared with the prior art, the present invention has the following advantages: The microcapsules described in the present invention use the processed eight-membered cucurbit ring as the microcapsule wall, and fill the cavity of the microcapsule wall with a bonding component. The addition of the bonding component improves the bonding efficiency with the microcapsule core material, and finally the microcapsule core material is sealed in the cavity by a combined plugging agent mode, thereby improving the overall stability and sustained release speed of the microcapsule and increasing the embedding rate of the core material. DETAILED DESCRIPTION

[0015] Unless otherwise defined, the technical terms used in the following examples have the same meanings as those generally understood by those skilled in the art to which the present invention belongs. The test reagents used in the following examples, unless otherwise specified, are all conventional biochemical reagents; the experimental methods, unless otherwise specified, are all conventional methods.

[0016] The present invention will be described in detail below with reference to the embodiments.

[0017] Example 1

[0018] A method for preparing microcapsules comprises the following steps: (1) adding 10 g of the eight-membered cucurbitacin into 1 L of a 6 mol / L hydrochloric acid solution, standing for 8 days, filtering, and drying to obtain the microcapsule wall; (2) immersing the obtained microcapsule wall in a binding component solution (a solution of chitosan and 50% branched maltodextrin in a mass ratio of 1:20) and stirring at 45° C. for 2 hours to obtain a treated wall material; (3) adding the treated wall material to a lactase solution (5 g of lactase in 100 mL of deionized water), stirring for 5 hours, filtering, and drying to obtain a wall material loaded with a core material; (4) Add the wall material of the core material to 1 L of 10% algae polysaccharide solution, mix well, add 1 g of tannic acid solution, stir at 70° C. for 2 hours, and then filter and dry to obtain the microcapsules.

[0019] Comparative Example 1 A method for preparing microcapsules comprises the following steps: (1) adding 10 g of the eight-membered cucurbitacin into 1 L of a 6 mol / L hydrochloric acid solution, standing for 8 days, filtering, and drying to obtain the microcapsule wall; (2) immersing the obtained microcapsule wall in a 50% aqueous solution containing branched maltodextrin, stirring at 45° C. for 2 hours, to obtain a treated wall material; (3) adding the treated wall material to a lactase solution (5 g of lactase in 100 mL of deionized water), stirring for 5 hours, filtering, and drying to obtain a wall material loaded with a core material; (4) Add the wall material of the core material to 1 L of 10% algae polysaccharide solution, mix well, add 1 g of tannic acid solution, stir at 70° C. for 2 hours, and then filter and dry to obtain the microcapsules.

[0020] Comparative Example 2 A method for preparing microcapsules comprises the following steps: (1) adding 10 g of the eight-membered cucurbitacin into 1 L of a 6 mol / L hydrochloric acid solution, standing for 8 days, filtering, and drying to obtain the microcapsule wall; (2) adding the treated wall material to a lactase solution (5 g of lactase in 100 mL of deionized water), stirring for 5 hours, filtering, and drying to obtain a wall material loaded with a core material; (3) Add the wall material of the core material to 1 L of 10% algae polysaccharide solution, mix well, add 1 g of tannic acid solution, stir at 70 ° C for 2 hours, and then filter and dry to obtain the microcapsule.

[0021] Comparative Example 3 A method for preparing microcapsules comprises the following steps: (1) adding 10 g of the eight-membered cucurbitacin into 1 L of a 6 mol / L hydrochloric acid solution, standing for 8 days, filtering, and drying to obtain the microcapsule wall; (2) immersing the obtained microcapsule wall in a binding component solution (a solution of chitosan and 50% branched maltodextrin in a mass ratio of 1:20) and stirring at 45° C. for 2 hours to obtain a treated wall material; (3) adding the treated wall material to a lactase solution (5 g of lactase in 100 mL of deionized water), stirring for 5 hours, filtering, and drying to obtain a wall material loaded with a core material; (4) Add the wall material of the core material to 1 L of 10% euglena polysaccharide solution, stir at 70° C. for 2 hours, and then filter and dry to obtain the microcapsules.

[0022] Comparative Example 4 (no blocking) A method for preparing microcapsules comprises the following steps: (1) adding 10 g of the eight-membered cucurbitacin into 1 L of a 6 mol / L hydrochloric acid solution, standing for 8 days, filtering, and drying to obtain the microcapsule wall; (2) immersing the obtained microcapsule wall in a binding component solution (a solution of chitosan and 50% branched maltodextrin in a mass ratio of 1:20) and stirring at 45° C. for 2 hours to obtain a treated wall material; (3) Add the treated wall material to a lactase solution (5 g of lactase in 100 mL of deionized water), stir for 5 hours, filter, and dry.

[0023] Comparative Example 5 A method for preparing microcapsules comprises the following steps: (1) adding 10 g of the eight-membered cucurbitacin into 1 L of a 6 mol / L hydrochloric acid solution, standing for 8 days, filtering, and drying to obtain the microcapsule wall; (2) Add the treated wall material to a lactase solution (5 g of lactase in 100 mL of deionized water), stir for 5 hours, filter, and dry.

[0024] The lactase loading rate of the products obtained in Example 1 and Comparative Examples 1-5 was tested by dissolving the products in deionized water, centrifuging at 10000 rpm for 1 min, and measuring the lactase activity after dilution of the supernatant. Loading rate = 100% - supernatant enzyme activity / total enzyme activity. The results are shown in Table 1.

[0025] Table 1 Load rate results

[0026] As shown in Table 1, the loading rates of the microcapsules obtained in Example 1 at different times are much higher than those of Comparative Examples 1-5. Compared with Example 1, in the absence of blocking components and bonding components, the loading rates of the microcapsules at different times in Comparative Example 5 are significantly reduced; in the absence of blocking components, Comparative Example 4 still has a certain loading effect by relying on the bonding component; Comparative Example 3 uses algae polysaccharide alone for blocking, and the loading effect is significantly reduced compared with Example 1; Comparative Example 2 seriously affects the loading effect of the microcapsules without adding the bonding component; In Comparative Example 1, chitosan is not added for bonding, which has a certain impact on the loading effect.

[0027] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for preparing microcapsules, characterized in that: The steps include: Step 1 is to immerse the microcapsule wall in a bonding component solution and stir it under heating conditions to obtain a treated wall material; Step 2 is to add the treated wall material to the microcapsule core material solution, stir, filter and dry to obtain the wall material loaded with the core material; Step 3 is to add the wall material of the core material to the first plugging component, mix them evenly, then add the second plugging component thereto, stir under heating conditions, and then filter and dry to obtain the microcapsules.

2. The method for preparing microcapsules according to claim 1, characterized in that: The microcapsule wall in step 1 is prepared by the following steps: adding the eight-membered cucurbitacin into an acid solution, standing, filtering and drying to obtain the microcapsule wall; the acid solution is a hydrochloric acid solution with a concentration of 1-10 mol / L; and the standing step lasts for 1-10 days.

3. The method for preparing microcapsules according to claim 1, characterized in that: The bonding component solution in step 1 is an aqueous solution of chitosan and branched maltodextrin in a mass ratio of 1:10-100.

4. The method for preparing microcapsules according to claim 3, characterized in that: The mass concentration of the aqueous solution containing branched maltodextrin is 30-60%; the molecular weight of the branched maltodextrin is less than or equal to 4500 g / mol; the deacetylation degree of the chitosan is 85-95%, and the molecular weight distribution is 50,000-200,000.

5. The method for preparing microcapsules according to claim 1, characterized in that: The temperature of the heating step in step 1 is 30-50° C., and the time of the stirring step is 1-10 hours.

6. The method for preparing microcapsules according to claim 1, characterized in that: The stirring step in step 2 is performed for 1 to 10 hours.

7. The method for preparing microcapsules according to claim 1, characterized in that: The first plugging component in step 3 is a euglena polysaccharide solution with a mass concentration of 1-20%, and the second plugging component is tannic acid; the solid-liquid ratio of the second plugging component to the first plugging component is 1g:1-10L.

8. The method for preparing microcapsules according to claim 1, characterized in that: The temperature of the heating step in step 3 is 50-80° C., and the time of the stirring step is 1-10 hours.

9. A microcapsule prepared by the preparation method according to any one of claims 1 to 8.

10. The use of the microcapsule according to claim 9, characterized in that: The application of the microcapsules in preparing drug capsule preparations or foods; the application of the microcapsules in preparing milk powder.

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