Biological fermentation soil conditioner and preparation method thereof

Abstract

The invention relates to the technical field of biological cells, and discloses a biologically fermented soil conditioner and a preparation method thereof.The soil conditioner is prepared from, by weight, 3 + / -2 parts of water dispersible nano sulfur-containing colloidal suspension, 70 + / -10 parts of compound amino acid solution, 20 + / -5 parts of compound ion mineral substance and 7 + / -2 parts of organic acid; according to the method, the soil conditioner is added into the soil, ionic mineral substances in the conditioner can rapidly promote ATP generation of indigenous microorganisms to promote microbial breeding gene change and shorten the breeding cycle, indigenous flora proliferation in the soil can be activated, the indigenous microorganisms are greatly proliferated to enable a soil layer to generate a fermentation effect, and the soil layer is protected from being damaged. The generated aerobic bacteria can multiply to kill anaerobic bacteria, and the multiplication of the aerobic bacteria not only can improve the soil fertility, potassium dissolving capacity, phosphorus dissolving capacity, salt dissolving capacity and nitrogen fixing capacity, but also can degrade heavy metal pollutants, so that the occurrence of soil-borne diseases is prevented and controlled, and the purposes of integrally repairing and improving the soil and increasing the crop yield are achieved.

Description

Technical Field

[0001] The invention relates to the technical field of biological cells, in particular to a biological fermentation soil improver and a preparation method thereof. Background Art

[0002] With the continuous development of agriculture, farmland soil problems are becoming increasingly serious, especially salinization, salinization, acidification and repeated cropping, which have had a serious impact on agricultural production. The traditional method usually adopts the method of farmland rest, that is, the farmland is idle for more than 3 years in order to improve the soil condition through natural recovery.

[0003] However, this method has obvious limitations: high time cost, it takes more than 3 years to prepare cultivated land, which is a big challenge for agricultural production; limited effect, even after a long period of preparation, it is often difficult to completely eliminate the salt, alkaline substances and harmful substances in the soil; waste of resources, during the preparation period, land resources are idle and cannot play their due production value; ecological risks, long-term fallow may lead to imbalance of soil ecosystem, reduction of beneficial microorganisms and deterioration of soil structure. Therefore, it is particularly important to develop an innovative technology that can effectively solve the problem of cultivated soil, shorten the repair time and improve the repair effect.

[0004] The present invention provides an innovative microbial technology route and soil fermentation improvement method, aiming to overcome the shortcomings of traditional methods and achieve rapid and efficient improvement of cultivated soil. Summary of the invention

[0005] 1. Technical issues to be resolved In view of the deficiencies in the prior art, the present invention provides a bio-fermented soil conditioner and a preparation method thereof, which has the advantage of being able to biodegrade salt in the soil, and solves the problems of salinization, salinization, acidification and repeated cropping of cultivated soil.

[0006] (II) Technical solution To achieve the above-mentioned purpose, the present invention provides the following technical scheme: a biological fermentation soil conditioner, wherein the raw materials of the conditioner and their weight ratios are: 3±2 weight ratio of water-dispersible nano-sulfur-containing gum suspension; 70±10 weight ratio of composite amino acid solution; 20±5 weight ratio of composite ion minerals; 7±2 weight ratio of organic acid.

[0007] Preferably, a method for preparing a biological fermentation soil conditioner is prepared according to the weight parts of the raw materials of the above-mentioned biological fermentation soil conditioner, and comprises the following preparation steps: Step 1, raw material preparation 1: preparation of water-dispersible nano sulfur-containing glue suspension; Step 2, raw material preparation 2: preparation of a mixed reaction of composite ion minerals, amino acid fermentation broth and organic acid; Step 3, mixing raw materials: taking the water-dispersible nano sulfur-containing glue suspension in step 1 at a weight ratio of 3±2 and mixing it with the stable solution in step 2, stirring and blending for 10-15 minutes, and the soil conditioner is prepared.

[0008] Preferably, the preparation process of the water-dispersible nano sulfur-containing glue suspension in step 1 is: S1.1. Prepare raw materials: prepare pure water, dispersing surfactant, water-soluble polymer and medical sulfur powder in the weight ratio of raw materials; S1.2, homogenization operation: add dispersing surfactant and water-soluble polymer into pure water, and use a homogenizer to homogenize to obtain a homogenous solution; S1.3, Nano preparation: Add the medical sulfur powder into the mixed solution obtained after dispersion, use the nano preparation process to prepare a water-dispersible nano sulfur-containing glue suspension with nano-level medical sulfur particles, and control the pH of the glue suspension between 6 and 8.

[0009] Preferably, the weight ratio of raw materials of the water-dispersible nano-sulfur-containing colloidal suspension in S1.1 is: 60-70 weight ratio of pure water; 0.3-3 weight ratio of dispersing surfactant; 1-5 weight ratio of water-soluble polymer; 30±5 weight ratio of medical sulfur powder.

[0010] Preferably, the particle size of the medical sulfur powder is between 250-350 meshes, and the medical sulfur powder is purified sulfur powder. The purification method is a permeation steam method, in which the sulfur powder is placed in a permeation steam device, steam is generated by heating, and the sulfur powder is sublimated into gas. The steam turns back into a solid after cooling, and pure sulfur powder, i.e., medical sulfur powder, is obtained by collecting and filtering.

[0011] Preferably, the homogenization conditions in S1.2 are: the temperature is controlled between 35-45°C, the homogenization speed is 8000-8500rpm, and the homogenization time is 25-30min.

[0012] Preferably, the nanoparticle size of the water-dispersible nano sulfur-containing glue suspension is in the range of 200±20.

[0013] Preferably, the mixed reaction preparation method of the composite ion minerals, amino acid fermentation liquid and organic acid is: S2.1, raw material preparation: prepare 70±10 weight ratio of complex amino acid solution; 20±5 weight ratio of complex ionic minerals; 7±2 weight ratio of organic acid; S2.2, mixing: after uniformly mixing the composite amino acid solution in the range of N*6.25, 30±5 weight ratio based on total nitrogen, adding the composite ion minerals; S2.3, Chelation reaction: Set the temperature, control the reaction time, and induce the chelation reaction of the complex ion minerals and the complex amino acids; S2.4. Add organic acid: Add organic acid to the solution where the chelation reaction occurs to stabilize the amino acid chelation reaction. After the chelation reaction is stable, it will be used for later use.

[0014] Preferably, the mixing conditions in S2.1 are: the temperature is controlled between 20-30° C., and the mixing time is controlled within the range of 10-15 min.

[0015] Preferably, the chelating reaction conditions in S2.2 are: controlling the temperature between 40-60° C. and the reaction time within the range of 40-80 min.

[0016] Compared with the prior art, the present invention provides a bio-fermented soil conditioner and a preparation method thereof, which has the following beneficial effects: 1. The present invention is to add a soil conditioner to the soil. The ionic minerals in the conditioner can not only quickly promote the ATP generation of indigenous microorganisms to promote the change of microbial reproduction genes and shorten the reproduction cycle, but also activate the proliferation of indigenous flora in the soil. The large-scale proliferation of indigenous microorganisms causes the soil layer to produce a fermentation effect. The generated aerobic bacteria can multiply to eliminate anaerobic bacteria (harmful bacteria and pathogenic bacteria), and use beneficial microorganisms to restrain harmful microorganisms. When the reproduction of beneficial microorganisms is enhanced, it can not only improve the soil fertility, potassium, phosphorus, salt and nitrogen fixation capabilities, but also degrade heavy metal pollutants, thereby preventing and controlling the occurrence of soil-borne diseases, and achieving the purpose of overall repair and improvement of soil and increasing crop yields. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a step diagram of the method of the present invention. DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0019] See also Figure 1 A bio-fermented soil conditioner, the raw materials of the conditioner and their weight ratios are: 3±2 weight ratio of water-dispersible nano-sulfur-containing gum suspension; 70±10 weight ratio of composite amino acid solution; 20±5 weight ratio of composite ion minerals; 7±2 weight ratio of organic acid.

[0020] Specifically, a method for preparing a biological fermentation soil conditioner is prepared according to the weight parts of the raw materials of the above-mentioned biological fermentation soil conditioner, and includes the following preparation steps: Step 1, raw material preparation 1: preparation of water-dispersible nano sulfur-containing glue suspension; Step 2, raw material preparation 2: preparation of a mixed reaction of composite ion minerals, amino acid fermentation broth and organic acid; Step 3, mixing raw materials: taking the water-dispersible nano sulfur-containing glue suspension in step 1 at a weight ratio of 3±2 and mixing it with the stable solution in step 2, stirring and blending for 10-15 minutes, and the soil conditioner is prepared.

[0021] Specifically, the preparation process of the water-dispersible nano sulfur-containing glue suspension in step 1 is: S1.1. Prepare raw materials: prepare pure water, dispersing surfactant, water-soluble polymer and medical sulfur powder in the weight ratio of raw materials; S1.2, homogenization operation: add dispersing surfactant and water-soluble polymer into pure water, and use a homogenizer to homogenize to obtain a homogenous solution; S1.3, Nano preparation: Add the medical sulfur powder into the mixed solution obtained after dispersion, use the nano preparation process to prepare a water-dispersible nano sulfur-containing glue suspension with nano-level medical sulfur particles, and control the pH of the glue suspension between 6 and 8.

[0022] Specifically, the weight ratio of the raw materials of the water-dispersible nano-sulfur-containing colloidal suspension in S1.1 is: 60-70 weight ratio of pure water; 0.3-3 weight ratio of dispersing surfactant; 1-5 weight ratio of water-soluble polymer; 30±5 weight ratio of medical sulfur powder.

[0023] Specifically, the particle size of the medical sulfur powder is between 250-350 meshes. The medical sulfur powder is purified sulfur powder (the purpose is to remove harmful sulfur components). The purification method is the permeation steam method. The sulfur powder is placed in a permeation steam device, and steam is generated by heating to sublime the sulfur powder into gas. The steam turns back into solid after cooling, and pure sulfur powder, namely medical sulfur powder, is obtained by collection and filtration.

[0024] Specifically, the homogenization conditions in S1.2 are: the temperature is controlled between 35-45°C, the homogenization speed is 8000-8500rpm, and the homogenization time is 25-30min.

[0025] Specifically, the nanoparticle size of the water-dispersible nano sulfur-containing glue suspension is in the range of 200±20.

[0026] Specifically, the mixed reaction preparation method of composite ion minerals, amino acid fermentation liquid and organic acid is as follows: S2.1, raw material preparation: prepare 70±10 weight ratio of complex amino acid solution; 20±5 weight ratio of complex ionic minerals; 7±2 weight ratio of organic acid; S2.2, mixing: after uniformly mixing the composite amino acid solution in the range of N*6.25, 30±5 weight ratio based on total nitrogen, adding the composite ion minerals; S2.3, Chelation reaction: Set the temperature, control the reaction time, and induce the chelation reaction of the complex ion minerals and the complex amino acids; S2.4. Add organic acid: Add organic acid to the solution where the chelation reaction occurs to stabilize the amino acid chelation reaction. After the chelation reaction is stable, it will be used for later use.

[0027] Specifically, the mixing conditions in S2.1 are: controlling the temperature between 20-30° C. and the mixing time within the range of 10-15 min.

[0028] Specifically, the chelating reaction conditions in S2.2 are: controlling the temperature between 40-60° C. and the reaction time within the range of 40-80 min.

[0029] When the soil conditioner prepared by the present invention is put into use, it has the principles of reducing soil salt, acidifying and solving the problem of repeated cropping, as follows: (1) Principle of soil salt reduction: After adding soil conditioners, a sufficient number of microorganisms are produced in the soil within a short period of time (10 to 20 days), and then a corresponding number of metabolic enzymes (dehydrogenase, dealdehydease) are obtained to achieve enzyme production and salt reduction. The principle of using soil conditioners is: because the formation process of metabolic enzymes will produce cations containing Fe²⁺, Fe³⁺, Fe(OH)²⁺, Fe(OH)2, Fe(OH)³⁺, Fe(OH)3, which participate in oxidation and reduction, replace various salts deposited in the soil, and convert insoluble salts into soluble nutrients that are easy for crops to absorb. Among them, the number of enzymes comes from the number of microorganisms.

[0030] The advantages are: by adding soil conditioners to cultivated land and introducing microbial communities of nitrogen-fixing bacteria, phosphate-solubilizing bacteria and potassium-solubilizing bacteria, the number of beneficial microorganisms in the soil can be increased, thereby improving soil structure and fertility. These microorganisms can decompose organic matter in the soil and release nutrients that can be absorbed by plants, while reducing the number of harmful microorganisms. The activity of microorganisms in the soil will promote the production of soil enzymes, which can participate in the biochemical reactions of organic matter decomposition and nutrient mineralization in the soil, thereby increasing the biochemical activity of the soil. The activity of microorganisms helps to form stable soil aggregates, improve the porosity and water retention capacity of the soil, make the soil more loose and breathable, and is conducive to the growth of the root system.

[0031] (2) Principle of acidification: After adding soil conditioners, a sufficient number of microorganisms will be produced in the soil within a short period of time (10 to 20 days). The metabolic process of these microorganisms can produce a large amount of oxygen, providing sufficient oxygen supply for the soil. Chemical formula: 4Fe(OH)3=4Fe(OH)2+2H2O+O2↑. This oxidation-reduction process will remove excess hydrogen ions in acidic soils or excess hydroxide ions in alkaline soils, thus achieving self-adjustment of soil acidity and alkalinity.

[0032] The advantages are: by adding soil conditioners, agricultural waste in the soil can be converted into organic fertilizer rich in beneficial microorganisms. This fertilizer can not only provide the nutrients needed for plant growth, but also improve the soil environment. Specific microorganisms can decompose the salt in the soil, reduce the impact of salinization on crops, and improve the cultivability of the soil. By utilizing the antagonism between microorganisms, the occurrence of soil-borne diseases and pests can be effectively inhibited, the use of chemical pesticides can be reduced, and the ecological environment can be protected.

[0033] (3) Principle for solving the problem of repeated cropping: After adding soil conditioners, soil fermentation is aerobic fermentation. Aerobic bacteria (beneficial bacteria) increase rapidly in this process, and kill harmful bacteria through natural warfare in the soil to restore the balance of the flora, thereby repairing the flora. Microorganisms help promote the release and chelation of trace elements in the soil through the exchange of oxygen and carbon dioxide and the secretion of acidic organic acids during metabolism. In the process of microbial reproduction and metabolism, they can degrade residual fertilizers, organic pesticides, heavy metals and other pollutants in the soil, indirectly promoting the availability of trace elements.

[0034] The advantages are: According to the effects of the above soil conditioners, specific implementation cases and comparative examples are compared: Example 1 (1) Turn the right side of the cultivated field A to be repaired and improved by 30 cm and use an irrigation hose to fill the field with water to 20 cm above the ground; (2) diluting the soil conditioner prepared by the raw material formula and preparation method of the present invention with 300 (liters / mu) of water at a ratio of 1:300, and injecting it into the field through a hose; (3) The ground dries up and green moss appears in about 5 days; (4) The ground turns red in 8 days; (5) After about 15 days, the ground turns back to earth color and fermentation is complete; (6) Prediction of improved acceptance index: EC value below 2, pH 6-6.5.

[0035] Comparative Example 1 The left side of the cultivated field A to be repaired and improved will be left fallow for 1 to 3 years.

[0036] Example 2 On the right side of the cultivated land B in different provinces, the scheme of Example 1 is re-implemented.

[0037] Comparative Example 2 On the left side of cultivated land B in different provinces, the plan of comparative example 1 is re-implemented.

[0038] Table 1 of the soil remediation effects of Example 1-2 and Comparative Example 1-2 is as follows: Table 1 date Example 1 Comparative Example 1 Remark July 3 Farmland A No change Farmland A No change At the beginning of the experiment, the left side is the field improved by soil fermentation method, and the right side is the control field. July 7 Experimental field light green Control field dark green The colors on both sides have changed within 4 days, indicating that the number of bacterial flora and salinity in the soil are different. July 13 Experimental field light red Control field red July 23 The experimental field has recovered its soil color (EC value below 1), and the improvement is complete The control field is still red (EC value above 4) This means that the field originally had problematic soil with a salinity of over 4. - Crop growth conditions in the soil fermentation improvement experimental field. - Improved crop growth - - Crop growth status in control field - date Example 2 Comparative Example 2 Remark - Left side of cultivated field B Right side of farmland B - - The cultivated grass is dense and dark green The cultivated land has sparse grass and is light green. Comparison of soil conditions during cultivation about one month after improvement was completed.

[0039] Table 1 shows that at the beginning of the experiment, the field layout was divided into a field improved by soil fermentation on the left and a control field on the right. As the experiment progressed to the 4th day, the improved field showed a light green color, while the control field remained dark green, indicating that the number of bacteria and salinity in the soil began to differ. On the 10th day, the improved field turned light red and the control field turned red, further reflecting the change in soil salinity. On the 20th day, the improved field had returned to its normal soil color (EC value below 1), indicating that the improvement had been completed; while the control field still showed red (EC value above 4), indicating that its soil salinity was high. At the same time, the crop growth in the improved field was good, while the crop growth in the control field was greatly affected by salinity. Example 2 and Comparison 2 also use the middle line separation method, with the improved field on the left and the control field on the right. About a month after the improvement was completed, the difference in soil color and texture between the improved field and the control field can be clearly seen.

[0040] The data comparing the salinity EC value, pH and microorganisms in the cultivated fields of the examples and the comparative examples measured in the laboratory are presented in Table 2 as follows: Table 2 project Example 1 Example 2 Comparative Example 1 Comparative Example 2 Salinity EC value 0.83 0.76 4.35 4.03 ph 6.1 6.3 4.6 3.6 Microbial growth on the first day Reproduce every 30 minutes Reproduce every 30 minutes Not bred Not bred Microbial growth on the tenth day \( N \times (2^{480} - 2^{240}) \) \( N \times (2^{480} - 2^{240}) \) The propagation rate is 0.05% of that in Example 1 The propagation rate is 0.08% of that in Example 2 The advantages are: the present invention adds a soil conditioner to the soil, and the ionic minerals in the conditioner can not only quickly promote the ATP generation of indigenous microorganisms to promote the change of microbial reproduction genes and shorten the reproduction cycle, but also activate the proliferation of indigenous flora in the soil. The large-scale proliferation of indigenous microorganisms causes the soil layer to produce a fermentation effect, and the generated aerobic bacteria can multiply to eliminate harmful bacteria and pathogens, and use beneficial microorganisms to restrain harmful microorganisms. When the reproduction of beneficial microorganisms is enhanced, not only can the soil fertility, potassium, phosphorus, salt and nitrogen fixation capabilities be improved, but also heavy metal pollutants can be degraded, thereby preventing and controlling the occurrence of soil-borne diseases, and achieving the purpose of overall repair and improvement of the soil and increasing crop yields.

[0041] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A biological fermentation soil conditioner, characterized in that: The improver raw materials and their weight ratios are: 3±2 weight ratio of water-dispersible nano sulfur-containing glue suspension; 70±10 weight ratio of composite amino acid solution; 20±5 weight ratio of composite ion minerals; 7±2 weight ratio of organic acid.

2. A method for preparing a biologically fermented soil conditioner, characterized in that: The biological fermentation soil conditioner according to claim 1 is prepared by weight of raw materials, comprising the following preparation steps: Step 1, raw material preparation 1: preparation of water-dispersible nano sulfur-containing glue suspension; Step 2, raw material preparation 2: preparation of a mixed reaction of composite ion minerals, amino acid fermentation broth and organic acid; Step 3, mixing raw materials: taking the water-dispersible nano sulfur-containing glue suspension in step 1 at a weight ratio of 3±2 and mixing it with the stable solution in step 2, stirring and blending for 10-15 minutes, and the soil conditioner is prepared.

3. The method for preparing a biological fermentation soil conditioner according to claim 2, characterized in that: The preparation process of the water-dispersible nano sulfur-containing glue suspension in step 1 is as follows: S1.

1. Prepare raw materials: prepare pure water, dispersing surfactant, water-soluble polymer and medical sulfur powder in the weight ratio of raw materials; S1.2, homogenization operation: add dispersing surfactant and water-soluble polymer into pure water, and use a homogenizer to homogenize to obtain a homogenous solution; S1.3, Nano preparation: Add the medical sulfur powder into the mixed solution obtained after dispersion, use the nano preparation process to prepare a water-dispersible nano sulfur-containing glue suspension with nano-level medical sulfur particles, and control the pH of the glue suspension between 6 and 8.

4. The method for preparing a biological fermentation soil conditioner according to claim 3, characterized in that: The weight ratio of the raw materials of the water-dispersed nano-sulfur-containing rubber suspension in S1.1 is: 60-70 weight ratio of pure water; 0.3-3 weight ratio of dispersing surfactant; 1-5 weight ratio of water-soluble polymer; 30±5 weight ratio of medical sulfur powder.

5. The method for preparing a biological fermentation soil conditioner according to claim 4, characterized in that: The particle size of the medical sulfur powder is between 250-350 meshes. The medical sulfur powder is purified sulfur powder. The purification method is a permeation steam method. The sulfur powder is placed in a permeation steam device, steam is generated by heating, and the sulfur powder is sublimated into gas. The steam turns back into solid after cooling, and pure sulfur powder, namely medical sulfur powder, is obtained by collection and filtration.

6. The method for preparing a biological fermentation soil conditioner according to claim 3, characterized in that: The homogenization conditions in S1.2 are: the temperature is controlled between 35-45°C, the homogenization speed is 8000-8500 rpm, and the homogenization time is 25-30 min.

7. The method for preparing a biological fermentation soil conditioner according to claim 3, characterized in that: The nanometer particle range of the water-dispersible nanometer sulfur-containing glue suspension is between 200±20.

8. The method for preparing a biological fermentation soil conditioner according to claim 2, characterized in that: The mixed reaction preparation method of the composite ion minerals, amino acid fermentation liquid and organic acid: S2.1, raw material preparation: prepare 70±10 weight ratio of complex amino acid solution; 20±5 weight ratio of complex ionic minerals; 7±2 weight ratio of organic acid; S2.2, mixing: after uniformly mixing the composite amino acid solution in the range of N*6.25, 30±5 weight ratio based on total nitrogen, adding the composite ion minerals; S2.3, Chelation reaction: Set the temperature, control the reaction time, and induce the chelation reaction of the complex ion minerals and the complex amino acids; S2.

4. Add organic acid: Add organic acid to the solution where the chelation reaction occurs to stabilize the amino acid chelation reaction. After the chelation reaction is stable, it will be used for later use.

9. The method for preparing a biological fermentation soil conditioner according to claim 8, characterized in that: The mixing conditions in S2.1 are: the temperature is controlled between 20-30° C., and the mixing time is controlled within the range of 10-15 min.

10. The method for preparing a biological fermentation soil conditioner according to claim 8, characterized in that: The chelating reaction conditions in S2.2 are: controlling the temperature between 40-60° C. and the reaction time between 40-80 min.

Images