Fermentation equipment and fermentation process of medicated leaven

Abstract

The invention discloses medicated leaven fermentation equipment and a fermentation process thereof. The medicated leaven fermentation equipment comprises a constant temperature and humidity chamber, a plurality of trays are arranged in the constant temperature and humidity chamber, and an oxygen control mechanism is mounted on one side of the constant temperature and humidity chamber; the oxygen control mechanism comprises a box body which is installed on the side face of the constant-temperature and constant-humidity box and communicated with the constant-temperature and constant-humidity box. According to the medicated leaven fermentation equipment and the fermentation process thereof provided by the invention, the oxygen control mechanism is mounted on the constant-temperature and constant-humidity chamber, so that the oxygen content in the constant-temperature and constant-humidity chamber can be conveniently controlled to be kept in a proper interval range through the oxygen control mechanism at different stages of medicated leaven fermentation; various fungi can better participate in the fermentation process, the negative influence of unstable change of the oxygen content on the fermentation process of the medicated leaven is reduced, the content and activity of various enzymes in the medicated leaven are improved, then the quality of medicated leaven of different batches is kept stable, and the prepared medicated leaven has the better digestion promoting characteristic.

Description

Technical Field

[0001] The present invention relates to the technical field of fermentation equipment, and in particular to a fermentation equipment for Liushenqu and a fermentation process thereof. Background Art

[0002] Liushenqu, also known as Liuqu and Shenqu, was first recorded in the Treatise on Materia Medica, which says: "It can transform food stagnation and stagnation, and strengthen the spleen and warm the stomach." It is a fermented mixture of bitter almonds, red beans, Artemisia annua, Xanthium sibiricum, Polygonum hydropiper, etc., mixed with flour and wheat bran. It is warm in nature, sweet and spicy in taste, and mainly enters the spleen and stomach meridians. It has the functions of digesting food, removing stagnation, strengthening the spleen and stomach, and is used for symptoms such as exogenous food damage, spleen deficiency and fullness, vomiting and diarrhea. It is clinically used to treat spleen and stomach weakness, food stagnation, chest distension and abdominal distension, and food accumulation in children, and is widely used.

[0003] The existing document with announcement number CN111388605B discloses a fermentation process of Liushenqu, which includes steps such as raw material preparation, pretreatment, pre-fermentation, concentration, mixing and molding, solid-state fermentation, and post-treatment, wherein a fermentation technology combining liquid pre-fermentation and solid-state fermentation is adopted; the invention also provides a Liushenqu prepared by the above method. The Liushenqu fermentation process in the invention is to prepare the Liushenqu preparation through two steps of pre-fermentation and fermentation, which has a simple process but a more thorough fermentation degree, and a short fermentation time and high fermentation efficiency; the Liushenqu prepared in the present invention has abundant digestive enzymes, and the digestive enzymes therein have good enzyme activity, so that Liushenqu has good effects on digestion, relieving stagnation, strengthening the spleen and stomach.

[0004] The document with announcement number CN113750122B discloses Liu Shen Qu and the preparation method of Liu Shen Qu. The purpose is to solve the technical problem of slow fermentation speed when preparing Liu Shen Qu in the prior art. The technical scheme adopted is: the preparation method of Liu Shen Qu includes the following steps: S1: Grind bitter almonds and red beans into powder, mix with flour and wheat bran to obtain pre-prepared powder; boil Polygonum hydropiper, Artemisia annua and Xanthium sibiricum in water for 1 hour, and concentrate the filtrate into a clear paste; S2: Mix the clear paste with the pre-prepared powder to obtain a fermentation substrate; S3: Sterilize the fermentation substrate;

[0005] S4: mixing the composite bacterial agent with the sterilized fermentation substrate, fermenting for 40 to 50 hours, and obtaining a fermentation product; S5: granulating and drying the fermentation product to obtain Liushenqu; wherein the composite bacterial agent includes: powdered Miller yeast, microscopic root Mucor, branched transverse stalk mold, red Aspergillus purpurogenus, spore-forming yeast, and Pichia burtonii. In addition, the invention also provides Liushenqu obtained by the above-mentioned preparation method.

[0006] It is well known that during the fermentation of Liu Shen Qu, the temperature and humidity need to be controlled, and there is no strict requirement for the control of oxygen content. Under different oxygen content, the activity of various bacteria is different, which can easily affect the activity of various enzymes (digestive enzymes) in Liu Shen Qu. Therefore, if the oxygen content during fermentation cannot be strictly controlled, the quality of different batches of Liu Shen Qu will easily become unstable.

[0007] Therefore, it is necessary to provide a fermentation equipment and fermentation process of Liushenqu to solve the above technical problems. Summary of the invention

[0008] In view of the above situation, in order to overcome the defects of the prior art, the present invention provides a fermentation equipment and fermentation process of Liu Shen Qu, which can keep the quality of Liu Shen Qu stable and have better digestion characteristics.

[0009] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0010] The fermentation equipment of Liu Shen Qu includes: a constant temperature and humidity chamber, a plurality of trays are arranged in the constant temperature and humidity chamber, the trays are used to place the fermentation raw materials of Liu Shen Qu, an oxygen control mechanism is installed on one side of the constant temperature and humidity chamber, in this embodiment, the oxygen control mechanism includes a box body installed on the side of the constant temperature and humidity chamber and connected thereto, an oxygen tank and a nitrogen tank are arranged on the box body, a first control valve and a second control valve are respectively installed between the oxygen tank, the nitrogen tank and the box body, a third control valve is installed on the top of the box body, external air can enter the constant temperature and humidity chamber through the control valve, an oxygen sensor is installed in the constant temperature and humidity chamber, the oxygen sensor detects the oxygen content in the constant temperature and humidity chamber, and displays the value through an external display.

[0011] Preferably, a control panel is installed on one side of the box body, and a display instrument is installed on the control panel. The display instrument is used to display the status of each control valve and the oxygen content, temperature and humidity in the constant temperature and humidity chamber. The control panel is connected to the first control valve, the second control valve and the third control valve.

[0012] Preferably, guide rails are installed on both sides of the constant temperature and humidity chamber, and plug-in parts adapted to the guide rails are provided on both sides of the tray.

[0013] Preferably, micro holes are provided at the bottom of the tray.

[0014] Preferably, a plurality of heating rods are installed in the constant temperature and humidity chamber, a temperature sensor and a humidity sensor are installed in the constant temperature and humidity chamber, a humidifier and an exhaust valve are installed on the top of the constant temperature and humidity chamber, and a refrigeration device is installed on the back of the constant temperature and humidity chamber.

[0015] A fermentation process of Liushenqu comprises the following steps: S1: preparing Liushenqu raw materials to be fermented, the preparation process of the Liushenqu raw materials to be fermented can be carried out according to the contents and steps disclosed in CN111388605B or CN113750122B, which will not be repeated here; S2: placing the raw materials to be fermented into a fermentation device of Liushenqu for fermentation for 2-5 days, specifically, spreading the raw materials to be fermented into a tray with a thickness not exceeding 2 cm, controlling the temperature at 26-31°C, the humidity at 75-85%, the oxygen content at 20-22% in the early stage of fermentation, the content at 14-20% in the middle stage of fermentation, and the content at 12-14% in the middle stage of fermentation, to obtain a fermentation product; S3: taking out the fermentation product, drying it, and cutting it into pieces, to obtain Liushenqu.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] (1) The present invention installs an oxygen control mechanism on the constant temperature and humidity chamber, and can conveniently control the oxygen content in the constant temperature and humidity chamber to be kept within an appropriate range at different stages of Liu Shenqu fermentation through the oxygen control mechanism, so that various fungi can better participate in the fermentation process, reduce the negative impact of unstable changes in oxygen content on the fermentation process of Liu Shenqu, and help to increase the content and activity of various enzymes in Liu Shenqu, thereby maintaining the quality of different batches of Liu Shenqu stable, and the prepared Liu Shenqu has better digestion characteristics;

[0018] (2) The present invention can conveniently control the temperature and humidity of the air in the constant temperature and humidity chamber by providing a heating rod, a humidifier, an exhaust valve and a refrigeration device;

[0019] (3) The present invention can conveniently monitor the internal conditions of the constant temperature and humidity chamber and conveniently control the opening and closing of the first control valve, the second control valve and the third control valve by installing a control panel and a display instrument on the chamber body;

[0020] (4) The present invention can conveniently support and place the tray by installing guide rails on both sides of the constant temperature and humidity chamber and providing plug-in parts on both sides of the tray;

[0021] (5) The present invention provides micropores at the bottom of the tray to enhance the air permeability of the tray, which is beneficial to increasing the metabolic rate of aerobic bacteria and improving the fermentation efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 A schematic diagram of the structure of the fermentation equipment of Liushenqu provided by the present invention;

[0023] Figure 2 for Figure 1 A schematic diagram of the top view of the fermentation equipment of Liushenqu is shown;

[0024] Figure 3 for Figure 1 The schematic diagram of the internal structure of the fermentation equipment of Liushenqu is shown;

[0025] Figure 4 for Figure 1 The schematic diagram of the structure of the oxygen control mechanism in the fermentation equipment of Liushenqu is shown;

[0026] Figure 5 for Figure 1 The schematic diagram of the structure of the tray in the fermentation equipment of Liushenqu is shown;

[0027] Figure 6 for Figure 1 The diagram shows the working principle of the oxygen control mechanism in the fermentation equipment of Liu Shenqu.

[0028] Among them, the names corresponding to the figure numbers are: 1-constant temperature and humidity chamber, 2-tray, 3-oxygen control mechanism, 4-box, 5-oxygen tank, 6-nitrogen tank, 7-first control valve, 8-second control valve, 9-third control valve, 10-control panel, 11-display instrument, 12-guide rail, 13-plug-in part, 14-micropore, 15-heating rod, 16-humidifier, 17-exhaust valve, 18-refrigeration equipment. DETAILED DESCRIPTION

[0029] The present invention is further described below in conjunction with the accompanying drawings and embodiments. The embodiments of the present invention include but are not limited to the following embodiments.

[0030] Embodiment 1:

[0031] like Figure 1-6As shown, the fermentation equipment of Liu Shen Qu provided by the present invention includes: a constant temperature and humidity box 1, a plurality of trays 2 are arranged in the constant temperature and humidity box 1, the trays 2 are used to place the fermentation raw materials of Liu Shen Qu, and an oxygen control mechanism 3 is installed on one side of the constant temperature and humidity box 1. In this embodiment, the oxygen control mechanism 3 includes a box body 4 installed on the side of the constant temperature and humidity box 1 and connected thereto, an oxygen tank 5 and a nitrogen tank 6 are arranged on the box body 4, a first control valve 7 and a second control valve 8 are respectively installed between the oxygen tank 5, the nitrogen tank 6 and the box body 4, and a third control valve 9 is installed on the top of the box body 4, and external air can enter the constant temperature and humidity box 1 through the control valve 9. Correspondingly, an oxygen sensor is installed in the constant temperature and humidity box 1, and the oxygen sensor detects the oxygen content in the constant temperature and humidity box 1 and displays the value through an external display. When in use, the Liu Shen Qu raw materials to be fermented are placed on the tray 2, so that the constant temperature and humidity box 1 is maintained at a constant temperature and humidity state, and then the oxygen content in the constant temperature and humidity box 1 is detected in real time by the oxygen sensor, and the oxygen content threshold is set according to the fermentation stage of Liu Shen Qu. In the early, middle and late fermentation stages of Liu Shen Qu, the oxygen content thresholds are different. When the oxygen content in the constant temperature and humidity box 1 is too low, the pure oxygen in the oxygen tank 5 or the external air can be introduced into the constant temperature and humidity box 1 by opening the first control valve 7 or the third control valve 9 to increase the oxygen content in the constant temperature and humidity box 1. When the oxygen content in the wet box 1 is too high, the second control valve 8 can be opened to pass the nitrogen in the nitrogen tank 6 into the constant temperature and humidity box 1 to reduce the oxygen content in the constant temperature and humidity box 1. The above measures can effectively ensure that the oxygen content of Liu Shen Qu in each stage of fermentation is always maintained within a stable range, so that the fungi in Liu Shen Qu can better participate in the fermentation process, and reduce the negative impact of the unstable changes in oxygen content on the fermentation process of Liu Shen Qu, which is beneficial to increase the content and activity of various enzymes in Liu Shen Qu and keep the quality of different batches of Liu Shen Qu stable.

[0032] By installing an oxygen control mechanism 3 on the constant temperature and humidity chamber 1, it is possible to conveniently control the oxygen content in the constant temperature and humidity chamber 1 to be maintained within an appropriate range at different stages of the fermentation of Liu Shen Qu through the oxygen control mechanism 3, so that various fungi can better participate in the fermentation process, and reduce the negative impact of unstable changes in oxygen content on the fermentation process of Liu Shen Qu, which is beneficial to increasing the content and activity of various enzymes in Liu Shen Qu, thereby keeping the quality of different batches of Liu Shen Qu stable.

[0033] Embodiment 2:

[0034] like Figure 4As shown, in this embodiment, a control panel 10 is installed on the front side of the box body 4, and a display instrument 11 is installed on the control panel 10. The display instrument 11 is used to display the status of each control valve and the oxygen content, temperature and humidity in the constant temperature and humidity chamber 1. The control panel 10 is connected to the first control valve 7, the second control valve 8 and the third control valve 9. The opening and closing of the first control valve 7, the second control valve 8 and the third control valve 9 can be manually or automatically controlled by the control panel 10, thereby facilitating the introduction of air, oxygen or nitrogen into the constant temperature and humidity chamber 1, which is more convenient to use.

[0035] By installing the control panel 10 and the display instrument 11 on the box body 4, it is convenient to monitor the internal conditions of the constant temperature and humidity box 1 and to control the opening and closing of the first control valve 7, the second control valve 8 and the third control valve 9.

[0036] Embodiment 3:

[0037] like Figure 3 and Figure 5 As shown, in this embodiment, guide rails 12 are installed on both sides of the constant temperature and humidity chamber 1, and plug-in parts 13 adapted to the guide rails 12 are provided on both sides of the tray 2. When in use, the plug-in parts 13 on both sides of the tray 2 are placed in the guide rails 12 and move forward or backward along the guide rails 12, so that the tray 2 can be conveniently placed in the constant temperature and humidity chamber 1 or taken out of the constant temperature and humidity chamber 1.

[0038] By installing the guide rails 12 on both sides of the constant temperature and humidity box 1 and providing the plug-in parts 13 on both sides of the tray 2, the tray 2 can be conveniently supported and placed.

[0039] Embodiment 4:

[0040] like Figure 5 As shown, in this embodiment, in order to enhance the air permeability of the tray 2, micropores 14 are opened at the bottom of the tray 2. When in use, the Liu Shen Qu raw materials are spread flat in the tray 2. Due to the presence of the micropores 14, the air fluidity at the bottom of the raw materials is enhanced, which is beneficial to increase the metabolic rate of some aerobic bacteria, and then produce more enzymes required for fermentation, which is beneficial to shorten the fermentation time and improve the fermentation efficiency.

[0041] By providing micropores 14 at the bottom of the tray 2, the air permeability of the tray 2 is enhanced, which is beneficial to increasing the metabolic rate of aerobic bacteria and improving the fermentation efficiency.

[0042] Embodiment 5:

[0043] like Figure 3As shown, a plurality of heating rods 15 are installed in the constant temperature and humidity chamber 1, a temperature sensor and a humidity sensor are installed in the constant temperature and humidity chamber 1, a humidifier 16 and an exhaust valve 17 are installed on the top of the constant temperature and humidity chamber 1, and a refrigeration device 18 is installed on the back of the constant temperature and humidity chamber 1. When in use, the temperature sensor and the humidity sensor detect the temperature and humidity in the constant temperature and humidity chamber 1, and the constant temperature and humidity chamber 1 is heated or cooled by the heating rods 15 and the refrigeration device 18 to keep the internal temperature constant and dehumidify the air, and the moisture is discharged from the exhaust valve 17. When the humidity inside the constant temperature and humidity chamber 1 is low, the air inside the constant temperature and humidity chamber 1 is humidified by the humidifier 16 to keep the air humidity constant.

[0044] By providing the heating rod 15 , the humidifier 16 , the exhaust valve 17 and the refrigeration device 18 , the temperature and humidity of the air in the constant temperature and humidity chamber 1 can be conveniently controlled.

[0045] A fermentation process of Liu Shen Qu, using the Liu Shen Qu fermentation equipment provided in the above embodiments for fermentation, comprises the following steps: S1: preparing Liu Shen Qu raw materials to be fermented, the preparation process of Liu Shen Qu raw materials to be fermented can be carried out according to the contents and steps disclosed in CN111388605B or CN113750122B, which will not be repeated here; S2: placing the raw materials to be fermented into the Liu Shen Qu fermentation equipment in embodiments 1-5 for fermentation, and fermenting for 2-5 days. Specifically, the raw materials to be fermented are spread flat into a tray 2 with a thickness not exceeding 2 cm, the temperature is controlled at 26-31°C, the humidity is controlled at 75-85%, the oxygen content in the early stage of fermentation is controlled at 20-22%, the content in the middle stage of fermentation is controlled at 14-20%, and the content in the middle stage of fermentation is controlled at 12-14%, so as to obtain a fermentation product; S3: taking out the fermentation product, drying it, and cutting it into pieces, so as to obtain Liu Shen Qu.

[0046] Select the same raw materials as those in CN111388605B and CN113750122B, and ferment according to the above fermentation process to obtain Liushenqu 1 and Liushenqu 2; according to the contents disclosed in CN111388605B and CN113750122B, obtain Liushenqu 3 and Liushenqu 4;

[0047] The enzyme activities of three digestive enzymes in Liushenqu 1, Liushenqu 2, Liushenqu 3 and Liushenqu 4, namely amylase, saccharifying enzyme and protease, were determined, wherein the determination method adopts the following method:

[0048] 1. Enzyme liquid preparation: weigh 5 g of each of the powders of Liushenqu 1, Liushenqu 2, Liushenqu 3 and Liushenqu 4, add 12.5 mL of pure water, prepare a 40 vol% solution, soak it in a 4°C refrigerator overnight, take it out, centrifuge it at 5000 rpm for 10 minutes, remove the supernatant to obtain the enzyme solution, and store it in a 4°C refrigerator for later use;

[0049] 2. Preparation of DNS reagent: Weigh 18.20g of potassium sodium tartrate and dissolve it in 50mL of pure water, heat it at 45℃, add 0.63g of 3,5-dinitrosalicylic acid while it is hot, prepare 2mol / L sodium hydroxide solution, take 26.20mL and add it to the above solution, weigh 0.51g of phenol and anhydrous sodium sulfate respectively and add them to the prepared solution, dilute to 100mL with distilled water, and store it at low temperature away from light for 7 to 10 days;

[0050] 3. Determination of amylase activity: The DNS colorimetric method was used to determine the amylase activity. The specific determination method was as follows: 250 μL of 1% soluble starch solution was added to a test tube, and then 100 μL of 1% NaCl solution was added. The mixture was kept at 37°C for 10 minutes. At the same time, the enzyme solution was preheated, and 50 μL of the enzyme solution was accurately drawn and added to the test tube. After the reaction was accurately timed for 15 minutes, 50 μL of 0.4 mol / L sodium hydroxide solution was immediately added to terminate the reaction. 1000 μL of ultrapure water was added to dilute the mixture, and finally 50 μL of DNS solution was added to develop color, and the mixture was boiled for 8 minutes to develop color. After cooling, the absorbance was measured at a wavelength of 540 nm. Definition of enzyme activity unit: The amylase activity is expressed as the mass of maltose produced by the enzyme catalysis in each gram of Liushenqu sample per minute, i.e., mg / (min·g). [0 079] The reference solution was treated in the same manner, except that 50 μL of the enzyme solution was replaced by 50 μL of 0.2 mg / mL maltose reference solution, which was divided into 6 parts and the average value was taken.

[0051] 4. Determination of saccharifying enzyme activity: The DNS colorimetric method was used to determine the activity of saccharifying enzyme. The specific determination method was as follows: 50 μL of enzyme solution was added with 100 μL of 2% soluble starch solution, and the solution was placed in a 50°C water bath for 1 h. Then, 150 μL of DNS reagent was added to dilute the solution to 2 mL, and the mixture was mixed and boiled for 5 minutes to develop color. The solution was cooled and the absorbance was determined at a wavelength of 540 nm. Definition of enzyme activity unit: The saccharifying enzyme activity was expressed as the mass of glucose produced by the enzyme catalysis in each gram of Liushenqu sample per minute, i.e., mg / (min·g).

[0052] The reference solution was treated in the same manner, except that 50 μL of the enzyme solution was replaced with 50 μL of 1.25 mg / mL glucose reference solution, which was divided into 6 equal portions and the average value was taken;

[0053] 5. Determination of protease activity: The activity of the protease was determined by the Folin phenol method. The specific determination method is as follows: take 125 μL of 0.65% casein solution and preheat it at 37°C for 5 minutes, then add 50 μL of enzyme solution, add 450 μL of purified water, mix well, then add 125 μL of 0.4 mol / L trichloroacetic acid solution, mix well, incubate in a 37°C water bath for 30 minutes, centrifuge, take 100 μL of supernatant, add 125 μL of 0.4 mol / L Na2CO3 solution, mix well, then add 100 μL of 2 mol / L Folin phenol reagent, mix well, incubate in a 37°C water bath for 30 minutes, and measure the absorbance at a wavelength of 660 nm; Definition of enzyme activity unit: Protease activity is expressed as the mass of nicotinic acid produced by enzyme catalysis in each gram of Liushenqu sample per minute, that is, mg / (min·g).

[0054] The reference solution was treated in the same manner, except that 50 μL of the enzyme solution was replaced with 50 μL of 1.10 mg / mL nortriptyline reference solution. The operation was repeated 6 times, the solution was divided into 6 equal parts, and the average value was taken.

[0055] 6. Enzyme activity determination results: The enzyme activity determination results in the above-mentioned Liushenqu are shown in Table 1 below.

[0056]

[0057] Table 1

[0058] It can be seen from Table 1 that the digestive enzyme activity of Liu Shen Qu 1 is greatly improved compared with Liu Shen Qu 3, and Liu Shen Qu 2 is improved compared with Liu Shen Qu 4. Therefore, the Liu Shen Qu produced by the equipment and process provided by the present invention has better digestive enzyme activity and thus has better digestion characteristics.

Claims

1. A fermentation device for Liushenqu, characterized in that: include: A constant temperature and humidity chamber (1), wherein a plurality of trays (2) are arranged inside the constant temperature and humidity chamber (1), and an oxygen control mechanism (3) is installed on one side of the constant temperature and humidity chamber (1); The oxygen control mechanism (3) comprises a box (4) installed on the side of the constant temperature and humidity box (1) and connected thereto, the box (4) is provided with an oxygen tank (5) and a nitrogen tank (6), a first control valve (7) and a second control valve (8) are respectively installed between the oxygen tank (5), the nitrogen tank (6) and the box (4), and a third control valve (9) is installed on the top of the box (4); An oxygen sensor is installed in the constant temperature and humidity box (1).

2. The fermentation equipment of Liushenqu according to claim 1, characterized in that: A control panel (10) is installed on one side of the box body (4), and a display instrument (11) is installed on the control panel (10).

3. The fermentation equipment of Liushenqu according to claim 1, characterized in that: Guide rails (12) are installed on both sides of the constant temperature and humidity box (1), and plug-in parts (13) adapted to the guide rails (12) are provided on both sides of the tray (2).

4. The fermentation equipment of Liushenqu according to claim 1, characterized in that: The bottom of the tray (2) is provided with microholes (14).

5. The fermentation equipment of Liushenqu according to claim 1, characterized in that: A plurality of heating rods (15) are installed in the constant temperature and humidity box (1), and a refrigeration device (18) is installed on the back of the constant temperature and humidity box (1).

6. The fermentation equipment of Liushenqu according to claim 1, characterized in that: A humidifier (16) and an exhaust valve (17) are installed on the top of the constant temperature and humidity box (1).

7. The fermentation equipment of Liushenqu according to claim 1, characterized in that: A temperature sensor and a humidity sensor are installed in the constant temperature and humidity box (1).

8. A fermentation process of Liushenqu, using the fermentation equipment of Liushenqu according to claims 1-7 for fermentation, characterized in that: The following steps are involved: S1: preparing Liushenqu raw materials to be fermented; S2: placing the raw materials to be fermented flat on the tray (2), controlling the temperature, humidity and oxygen content during fermentation, setting different oxygen content standards according to the fermentation stage, and fermenting for 2-5 days to obtain a fermentation product; S3: Take out the fermentation product, dry it, and cut it into pieces to obtain Liushenqu.

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