A strain of Fusarium acuminate and its application

Abstract

The present invention discloses a strain of Fusarium oxysporum and its application, and belongs to the technical field of plant endophytic fungi. The present invention screens and obtains a strain of plant endophytic fungi: Fusarium oxysporum G3‑23, with a deposit number of CGMCC No. 41495. Experiments have found that Fusarium oxysporum G3‑23 has excellent inhibitory effects on multiple pathogens such as Helicoverpa spp., Helicoverpa zeae, Fusarium graminearum, and Fusarium asiatica, indicating that Fusarium oxysporum G3‑23 has broad-spectrum antibacterial activity. The Fusarium oxysporum G3‑23 has significant biological control effects on leaf spot of gramineous grass, leaf spot of corn, and fusarium head blight of wheat. The present invention provides new strain resources for biological control of plant diseases and lays a theoretical foundation for the effective prevention and control of plant diseases.

Description

Technical Field

[0001] The invention relates to the technical field of plant endophytic fungi, in particular to a strain of Fusarium acuminate and application thereof. Background Art

[0002] Biological control has become an important means of plant disease control, with advantages such as environmental protection, low resistance, good sustained control effect, promotion of biodiversity and significant economic benefits. Plant endophytic fungi are a potential biological control resource that can control various plant pathogens and promote plant growth and health. These fungi are interdependent with their hosts without causing any obvious disease symptoms. Plant endophytic fungi are able to adapt to life inside plants and produce antagonistic secondary metabolites that are essential for the growth and disease resistance of host plants. Among plant endophytic fungi, fungi such as Penicillium, Epicoccum, Trichoderma, Fusarium, Chaetomium and Piriformospora can enhance the host's tolerance to biotic and abiotic stresses. However, there are few reports on Fusarium acuminatum in the control of plant fungal diseases.

[0003] Bipolaris sorokiniana is one of the most common and serious pathogens that cause plant fungal diseases. This pathogen can cause serious diseases of many grasses and plants such as barley, wheat, and rice, leading to leaf spots, root rot, mildew, etc., and the disease distribution area is very wide, which will cause huge economic losses. Bipolaris maydis can cause corn leaf diseases worldwide, often leading to poor growth of corn and affecting yield. Especially in years with severe disease, corn is infected on a large scale, plant growth is affected, yield is reduced, and high and stable yields of corn are affected, causing serious economic losses. Fusarium Head Blight (FHB) caused by Fusarium graminearum and Fusarium asiaticum is a global disease and one of the main diseases in my country's wheat production. Wheat fusarium head blight not only leads to reduced yields, but also the toxins produced by the pathogen reduce the quality of wheat and endanger the health of humans and livestock. The prevention and control of wheat fusarium head blight is mainly carried out by chemical control during the flowering period of wheat, but the pesticide control has caused serious drug resistance in the pathogen, so it is particularly important to explore biological control strategies. At present, biological control has become a research hotspot for the prevention and control of plant diseases, and has a very broad application prospect. Summary of the invention

[0004] The purpose of the present invention is to provide a strain of Fusarium oxysporum and its application to solve the problems existing in the above-mentioned prior art. The Fusarium oxysporum has excellent inhibitory effect on wheat root decay helminth fungus, maize helminth fungus, graminearum and asiatic Fusarium, and has broad-spectrum antibacterial activity. The strain has significant biological control effect on grass leaf spot disease, corn leaf spot disease and wheat fusarium ergot disease.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] The present invention provides a strain of Fusarium acuminatum G3-23, the preservation number of the Fusarium acuminatum G3-23 is CGMCC No.41495, the preservation time is September 4, 2024, the preservation unit is the General Microbiology Center of China Microbiological Culture Collection Administration, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0007] The present invention also provides a bacterial agent, comprising the Fusarium acuminate.

[0008] The present invention also provides the use of the Fusarium acuminate in any of the following items:

[0009] (1) Application in the prevention and control of grass plant diseases;

[0010] (2) Application in the preparation of microbial agents for preventing and controlling diseases of gramineous plants;

[0011] Wherein, the gramineous plant diseases include Elymus salsa leaf spot, corn leaf spot and wheat fusarium head blight.

[0012] In the embodiments of the present invention, the gramineous plant diseases are exemplified by Elymus leaf spot, corn leaf spot and wheat scab, but the gramineous plant diseases of the present invention are not limited thereto.

[0013] The present invention also provides the use of the Fusarium acuminate in any of the following items:

[0014] (1) Application in inhibiting pathogens of grass plant diseases;

[0015] (2) Application in the preparation of microbial agents for inhibiting pathogenic bacteria of grass plant diseases;

[0016] The pathogens of grass plant diseases include Bipolaris sorokiniana, Bipolaris maydis, Fusarium graminearum and Fusarium asiaticum.

[0017] The present invention also provides the use of the bacterial agent in preventing and treating diseases of gramineous plants, wherein the diseases of gramineous plants include leaf spot of lycopersicon esculentum, leaf spot of corn and fusarium head blight of wheat.

[0018] The present invention also provides the use of the bacterial agent in inhibiting pathogenic bacteria of gramineous plant diseases, wherein the pathogenic bacteria of gramineous plant diseases include Helminthosporium graminearum, Helminthosporium maydis, Fusarium graminearum and Fusarium asiatica.

[0019] The present invention also provides a method for preventing and controlling diseases of gramineous plants, comprising applying the acutus fusarium or the bacterial agent to gramineous plants, wherein the diseases of gramineous plants include leaf spot of lycopersicon esculentum, leaf spot of corn and fusarium head blight of wheat.

[0020] The present invention also provides a method for inhibiting pathogenic bacteria of Gramineae plants, comprising applying the acutus Fusarium or the bacterial agent to the pathogenic bacteria of Gramineae plants, wherein the pathogenic bacteria of Gramineae plants include Helminthosporium graminis, Helminthosporium maydis, Fusarium graminearum and Fusarium asiatica.

[0021] The present invention discloses the following technical effects:

[0022] The present invention screened and isolated a plant endophytic fungus, Fusarium acuminate G3-23, and found that strain G3-23 had excellent inhibitory effects on multiple pathogens such as Helminthosporium spp., Helminthosporium spp., Fusarium graminearum, and Fusarium asiatica, indicating that strain G3-23 had broad-spectrum antibacterial activity. Under greenhouse and field conditions, the spore suspension of strain G3-23 was applied to the leaves of Elymus salsa, which had a significant biological control effect on the leaf spot of Elymus salsa; the spore suspension of strain G3-23 was applied to corn leaves, which had a significant biological control effect on corn leaf spot; the spore suspension of strain G3-23 was applied to wheat ears, which had a significant biological control effect on wheat scab. It shows that strain G3-23 of the present invention has good application potential and prospects for the prevention and control of diseases of Gramineae plants. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0024] Figure 1 The colony and conidia morphology of strain G3-23; A is the top view of the colony morphology of G3-23 after five days of culture in PDA medium, B is the bottom view of the colony morphology of G3-23 after five days of culture in PDA medium, and C is the conidia morphology of G3-23. The scale bar is 50 μm.

[0025] Figure 2 The figure is the electrophoresis diagram of PCR amplification of the internal transcribed spacer (ITS) and elongation factor-1α gene (EF-1α) of strain G3-23; wherein, DNA Marker is a standard DNA molecule, ITS is the product amplified by primer pair ITS1 and ITS4, and EF is the product amplified by primer pair EF1 and EF2;

[0026] Figure 3 is the phylogenetic tree of strain G3-23;

[0027] Figure 4 The results of the broad-spectrum antibacterial activity test of strain G3-23 against pathogenic fungi; A is the antibacterial activity of strain G3-23 against plant pathogenic fungi on PDA medium (Bs is Helminthosporium graminis, Bm is Helminthosporium maydis, Fg is Fusarium graminearum, and Fa is Fusarium asiatica), B is the radial growth radius of the colony determined by the confrontation culture method, and C is the fungal colony growth inhibition rate determined by the confrontation culture method; the error bars represent the standard error of the mean; according to the student t test, significant differences with p≤0.05 are indicated by *, or according to the one-way analysis of variance and Tukey post hoc test, significant differences are indicated by *;

[0028] Figure 5 The control effect of treating Elymus dahliae plants with spores of strain G3-23 on leaf spot disease of Elymus dahliae in Example 4; A is a phenotypic diagram, and B is a statistical diagram;

[0029] Figure 6 The control effect of treating Elymus dahliae plants with spore solution of strain G3-23 on leaf spot disease of Elymus dahliae in Example 5; A is a phenotypic diagram, and B is a statistical diagram of the data;

[0030] Figure 7The control effect of corn leaf spot disease on corn plants treated with spore liquid of strain G3-23 in Example 6; A is a phenotypic diagram, and B is a statistical diagram;

[0031] Figure 8 The control effect of wheat fusarium rust on wheat plants treated with spores of strain G3-23 in Example 7; A is a phenotypic diagram, and B is a statistical diagram;

[0032] Fig. 9 The control effect of wheat fusarium rust on wheat plants treated with spores of strain G3-23 in Example 8; A is a phenotypic diagram, and B is a statistical diagram;

[0033] Fig.10 This is an analysis of the pathogenicity of strain G3-23 to Elymus salsa, corn or wheat under greenhouse conditions, wherein A is a representative picture of Elymus salsa plants treated with spore solution of strain G3-23 after growing in the greenhouse for 45 days, B is a representative picture of corn plants treated with spore solution of strain G3-23 after growing in the greenhouse for 45 days, and C is a representative picture of wheat plants treated with spore solution of strain G3-23 after growing in the greenhouse for 45 days. DETAILED DESCRIPTION

[0034] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0035] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0036] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0037] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0038] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0039] In the present invention, unless otherwise specified, the raw materials and equipment used can be purchased from the market or are commonly used in the art. The methods in the following embodiments, unless otherwise specified, are all conventional methods in the art.

[0040] The culture medium involved in the following examples is:

[0041] (1) Potato dextrose agar medium (PDA medium): Cut 200g of potatoes into small pieces and put them into a pot. Add 1000mL of water and heat to boiling on a heater for 30 minutes. Filter through a measuring cup with two layers of gauze while hot and discard the residue. Add 20g of glucose and 20g of agar to the filtrate and add water to 1000mL. Then put it into a high-temperature and high-pressure sterilizer for sterilization at 121℃ for 15 minutes.

[0042] (2) CMC medium: 15 g sodium carboxymethyl cellulose, 2 g sodium nitrate, 1 g potassium dihydrogen phosphate, 0.5 g magnesium sulfate heptahydrate and 1 g yeast extract, add water to 1000 mL. Then sterilize in a high-temperature and high-pressure autoclave at 121°C for 15 min.

[0043] Example 1 Isolation and identification of strains

[0044] 1. Strain Isolation

[0045] Healthy symptom-free wheat was collected in Heilongjiang Province, China. The surface was first disinfected by soaking the samples in 70% alcohol for 1 min, then transferred to 2% sodium hypochlorite for 2 min, and finally soaked in 70% alcohol for 30 seconds. After this treatment, it was washed three times with sterile distilled water and placed on sterile filter paper for further drying. The samples were cut into small fragments (about 5 mm) and placed on the surface of PDA culture medium. Ampicillin (50 μg / mL) was added to the culture medium to inhibit the growth of bacteria in PDA culture medium. 100 μL of the water washed in the last step of surface disinfection was also added to the surface of PDA culture medium to evaluate the efficiency of surface sterilization. All culture media were cultured in a dark incubator at 25°C. After the mycelium grew out, the mycelium at the tip of the colony was taken and transferred to a new PDA plate without antibiotics for preliminary purification culture. The colony was subcultured 3 times to obtain a pure strain, named strain G3-23. The cultured strains were stored on PDA slants at 4°C and simultaneously placed in 25% glycerol at -80°C for further use.

[0046] 2. Morphological and molecular biological identification of strain G3-23

[0047] 2.1 Morphological identification of G3-23

[0048] The preserved G3-23 strain was activated and cultured in PDA medium at 25°C in the dark for 5 days. Then, a 5 mm diameter bacterial cake was taken from the edge of the activated culture colony with a hole puncher and placed on a freshly prepared PDA medium and cultured for 5 days under the same culture conditions. The front and back of the colony were observed and photographed. Figure 1 As shown in A and B.

[0049] 2.2 Molecular biological identification

[0050] 2.2.1 DNA extraction and PCR amplification

[0051] After strain G3-23 was cultured in PDA medium for 7 days, genomic DNA was extracted using DNeasy Plant Mini kit (purchased from Qingdao Shengke Biotechnology Co., Ltd.). The concentration of the extracted DNA was determined using a NanoDrop spectrometer. The total volume of the specific PCR reaction system was 20 μL, containing 1 μL DNA template, 1 μL of each primer (Table 1), 10 μL of 2× Taq Mix (Quanshijin, Beijing, China) and 7 μL ddH2O. The reaction system and reaction procedure of specific PCR primers are shown in Tables 2 and 3. The PCR products were separated and purified using 1% agarose gel (w / v) containing 0.01% (v / v) GoldView nucleic acid stain, and electrophoresis was performed at 120V, 400A for 30min. The amplified bands were observed under UV light using a gel imaging system (BioRad, Chemi Doc, MP), and the electrophoresis results are shown in Tables 2 and 3. Figure 2 The single band was sent to Beijing Qingke Biotechnology Co., Ltd. for purification and sequencing.

[0052] Table 1 PCR amplification primer sequences

[0053]

[0054] Table 2 PCR reaction system

[0055]

[0056] Table 3 PCR reaction program

[0057]

[0058] 2.2.2 Phylogenetic evaluation of endophytic fungus strain G3-23

[0059] According to the sequencing results and NCBI alignment results, strains related to Fusarium were selected to construct a phylogenetic tree. The sequence of EF-1α was individually aligned using the maximum likelihood method based on the Tamura 3-parameter model for multi-locus linkage, and 1000 repeated bootstrap analyses were performed to estimate evolutionary distances. The phylogenetic relationships were as follows Figure 3 shown. Figure 3 This is the phylogenetic tree of strain G3-23 and its closely related species calculated by the maximum likelihood method based on EF-1α sequences. The results show that strain G3-23 is classified into one branch with several strains of Fusarium acuminatum, indicating that strain G3-23 belongs to Fusarium acuminatum.

[0060] The above-mentioned Fusarium acuminatum G3-23 was deposited in the General Microbiology Center of China Culture Collection Administration on September 4, 2024. The deposit address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC No.41495.

[0061] Example 2 Antibacterial Experiment

[0062] 1. Pathogenic fungi materials

[0063] Bipolaris sorokiniana, Bipolarismaydis, Fusarium graminearum and Fusarium asiaticum. All the above pathogens are deposited in the Institute of Plant Protection, Chinese Academy of Agricultural Sciences.

[0064] 2. Experimental methods

[0065] The inhibitory effect of strain G3-23 on Helminthosporium spp., Helminthosporium spp., Fusarium graminearum and Fusarium asiatica was determined by plate confrontation, as follows:

[0066] The radial growth radius of the colony and the inhibition rate of fungal colony growth were determined by the confrontation culture method. A 5mm diameter cake was taken from the edge of the pathogenic fungal colony after 5 days of activation and inoculated in the center of the PDA plate. A 5mm diameter cake was taken from the edge of the G3-23 colony that was also activated and inoculated on both sides close to the edge of the plate. The inoculated G3-23 cake was in a straight line with the plant pathogenic fungal cake. All culture dishes were placed in a 25℃ incubator and cultured in the dark for 7 days. The experiment was carried out 3 times, with 6 replicates for each treatment. The inhibitory effect of endophytes on pathogenic fungi was determined by observing and counting the growth of pathogenic fungi.

[0067] like Figure 4 As shown in Figure 2, on PDA medium, the endophytic fungus strain G3-23 had a strong inhibitory effect on four pathogenic fungi including Helminthosporium spp. (see Figure 4 (A) The radial growth radius and inhibition rate of the colony are shown in Figure 4 B. The inhibition rate of endophytic fungus G3-23 against Helminthosporium spp. was 50.6%, against Helminthosporium spp. was 64.9%, against Fusarium graminearum was 59.7%, and against Fusarium asiatica was 61.1% (see Figure 4 In summary, the inhibition rate of G3-23 against several pathogenic fungi can reach up to 64.9%.

[0068] Example 3 Preparation of conidia solution of strain G3-23

[0069] After strain G3-23 has grown in PDA medium at 25°C for 7 days, use a toothpick to pick up 7 to 10 bacterial blocks with a diameter of 5 mm and place them in CMC liquid medium. Incubate for 3 to 5 days in a shaker at 25°C and 180 rpm. Use a sterilized filter cloth to filter the above liquid into a sterilized 50 mL centrifuge tube to remove impurities such as culture medium and bacterial blocks. Centrifuge at a centrifugal force of 10,000 × g for 10 minutes to obtain a spore precipitate. Resuspend the spore precipitate with sterile water to obtain a concentrated spore solution. Then use a hemacytometer to measure the spore concentration and adjust the spore concentration to a working concentration (10 7 Spores / mL) were stored. Spore morphology was observed as follows Figure 1 As shown in C.

[0070] Example 4 Functional determination of strain G3-23 in controlling leaf spot of Elymus salsa

[0071] The spore liquid prepared in Example 3 was used as an example for determination, as follows:

[0072] Spray inoculation: Use a handheld atomizer to spray approximately 5 mL of G3-23 spore suspension (1×10 7 Spores mL -1 ) was sprayed on the leaves of different Elymus dahliae until a large number of droplets were deposited on the leaves of Elymus dahliae. Two days after application, the treated leaves of Elymus dahliae were sprayed with a suspension of conidia of Helicoverpa spp. (1×10 4 Spores mL -1 ). Sterile distilled water was used instead of G3-23 spore suspension as a control (Control). Cover the leaves of Elymus dahliae with black plastic bags for the first two days to maintain humidity and dark conditions. 15 days after inoculation, count the number and area of ​​diseased leaf spots on each inoculated Elymus dahliae leaf, record and take photos (such as Figure 5 The results showed that spraying the spore suspension of strain G3-23 2 days in advance could significantly reduce the number of diseased leaves and leaf spot area of ​​Elymus dahliae (p≤0.05), with a control efficiency of 68.68%.

[0073] Example 5 Functional determination of strain G3-23 in controlling leaf spot of Elymus salsa

[0074] The spore liquid prepared in Example 3 was used as an example for determination, as follows:

[0075] Spray inoculation: Use a handheld atomizer to spray approximately 5 mL of G3-23 spore suspension (1×10 7 Spores mL -1) was sprayed on different Elymus dahliae leaves until a large number of droplets were deposited on the leaves. Two days after application, the treated leaves of Elymus dahliae were sprayed with a conidia suspension of Bipolaris maydis (1×10 4 Spores mL -1 ). Sterile distilled water was used instead of G3-23 spore suspension as a control (Control). Cover the leaves of Elymus dahliae with black plastic bags for the first two days to maintain humidity and dark conditions. 15 days after inoculation, count the number and area of ​​diseased leaf spots on each inoculated Elymus dahliae leaf, record and take photos (such as Figure 6 The results showed that spraying the spore suspension of strain G3-23 2 days in advance could significantly reduce the number of diseased leaves and leaf spot area of ​​Elymus dahliae (p≤0.05), with a control efficiency of 63.07%.

[0076] Example 6 Functional determination of strain G3-23 in controlling corn leaf spot

[0077] The spore liquid prepared in Example 3 was used as an example for determination, as follows:

[0078] Spray inoculation: Use a handheld atomizer to spray approximately 5 mL of G3-23 spore suspension (1×10 7 Spores mL -1 ) was sprayed on different corn leaves until a large number of droplets were deposited on the corn leaves. Two days after application, the treated corn leaves were sprayed with a conidia suspension of Bipolaris maydis (1×10 4 Spores mL -1 ). Sterile distilled water was used instead of G3-23 spore suspension as a control. The corn leaves were covered with black plastic bags for the first two days to maintain humidity and dark conditions. 15 days after inoculation, the number and area of ​​leaf spots on each inoculated corn leaf were counted, recorded and photographed (e.g. Figure 7 The results showed that spraying the spore suspension of strain G3-23 2 days in advance could significantly reduce the number of diseased leaves and leaf spot area of ​​corn (p≤0.05), with a control efficiency of 77.14%.

[0079] Example 7 Functional determination of strain G3-23 in controlling wheat scab

[0080] The spore liquid prepared in Example 3 was used as an example for determination, as follows:

[0081] Spray inoculation: Use a handheld atomizer to spray approximately 5 mL of G3-23 spore suspension (1×10 7 Spores mL -1) was sprayed on wheat ears at the flowering stage until a large number of droplets were deposited on the wheat ears. Two days after application, the treated wheat ears were inoculated with a conidia suspension of Fusarium graminearum PH-1 (1×10 4 Spores mL -1 ). Sterile distilled water was used instead of G3-23 spore suspension as control. The wheat ears were covered with plastic bags for the first two days to maintain humidity. 15 days after inoculation, the number of diseased spikelets on each inoculated wheat ear was counted, recorded and photographed (e.g. Figure 8 The results showed that spraying the spore suspension of strain G3-23 2 days in advance had a significant control effect on wheat fusarium head blight, which could significantly reduce the number of wheat spikelets with disease (p≤0.05), and the control efficiency was as high as 76.94%.

[0082] Example 8 Functional determination of strain G3-23 in controlling wheat scab

[0083] The spore liquid prepared in Example 3 was used as an example for determination, as follows:

[0084] Spray inoculation: Use a handheld atomizer to spray approximately 5 mL of G3-23 spore suspension (1×10 7 Spores mL -1 ) was sprayed on wheat ears at the flowering stage until a large number of droplets were deposited on the wheat ears. Two days after application, the treated wheat ears were inoculated with a conidia suspension of Fusarium asiatica (1×10 4 Spores mL -1 ). Sterile distilled water was used instead of G3-23 spore suspension as control. The wheat ears were covered with plastic bags for the first two days to maintain humidity. 15 days after inoculation, the number of diseased spikelets on each inoculated wheat ear was counted, recorded and photographed (e.g. Fig. 9 The results showed that spraying the spore suspension of strain G3-23 2 days in advance had a significant control effect on wheat fusarium head blight, which could significantly reduce the number of wheat spikelets with disease (p≤0.05), and the control efficiency was as high as 60.13%.

[0085] In Examples 4 to 8, after the spore liquid of strain G3-23 was directly used to treat plants for 45 days, it was found that G3-23 was not pathogenic to Elymus dahliae, corn leaves, and wheat ears. Fig.10 .

[0086] The embodiments described above are only descriptions of the preferred embodiments of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A strain of Fusarium acuminatum, characterized in that: The deposit number of the Fusarium spp. is CGMCC No.41495.

2. A bacterial agent, characterized in that The invention comprises the Fusarium spp. described in claim 1.

3. Use of the Fusarium acuminate according to claim 1 in any of the following: (1) Application in the prevention and control of grass plant diseases; (2) Application in the preparation of microbial agents for preventing and controlling diseases of gramineous plants; in, The grass plant diseases include Elymus leaf spot, corn leaf spot and wheat fusarium head blight.

4. Use of the Fusarium spp. as claimed in claim 1 in any of the following: (1) Application in inhibiting pathogens of grass plant diseases; (2) Application in the preparation of microbial agents for inhibiting pathogenic bacteria of grass plant diseases; in, The pathogenic bacteria of gramineous plant diseases include Bipolaris sorokiniana, Bipolaris maydis, Fusarium graminearum and Fusarium asiaticum.

5. The use of the microbial agent as claimed in claim 2 in preventing and controlling diseases of Gramineae plants, characterized in that: The grass plant diseases include Elymus leaf spot, corn leaf spot and wheat fusarium head blight.

6. Use of the microbial agent according to claim 2 in inhibiting pathogenic bacteria of gramineous plant diseases, characterized in that: The pathogenic bacteria of grass plant diseases include Helminthosporium graminearum, Helminthosporium maydis, Fusarium graminearum and Fusarium asiatica.

7. A method for preventing and controlling diseases of grass plants, characterized in that: The method comprises applying the Fusarium solani described in claim 1 or the fungal agent described in claim 2 to gramineous plants, wherein the diseases of the gramineous plants include leaf spot of Elymus salsa, leaf spot of corn and fusarium head blight of wheat.

8. A method for inhibiting pathogenic bacteria of Gramineae, characterized in that: The method comprises applying the Fusarium solani of claim 1 or the bacterial agent of claim 2 to pathogenic bacteria of the Gramineae family, wherein the pathogenic bacteria of the Gramineae family include Helminthosporium graminis, Helminthosporium maydis, Fusarium graminearum and Fusarium asiatica.

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